Preservative-free soothing, moisturizing and mild hand and mouth wet tissue for infants and children and preparation method of hand and mouth wet tissue

Abstract

The invention relates to a preservative-free soothing, moisturizing and mild infant hand and mouth wet tissue and a preparation method thereof, the preservative-free soothing, moisturizing and mild infant hand and mouth wet tissue is characterized in that the infant hand and mouth wet tissue is composed of a base cloth and a wet tissue liquid, the wet tissue liquid is composed of the following components in percentage by mass: 0.48-0.90% of 1, 2-hexanediol, 0.15-0.29% of p-hydroxyacetophenone, 0.10-0.15% of caprylyl glycol, 0.13-0.24% of methylpropanediol, 0.04-0.08% of ethylhexylglycine, 5.00% of 1, 3-butanediol, 0.10% of EDTA disodium, 0.20-0.50% of D-panthenol, and the balance of water. 0-0.01% of tremella polysaccharide and the balance of pure water; the base cloth is selected from spunlace non-woven fabric and is made of one or more of pure cotton or full viscose or wood pulp composite or plant fiber. The invention has the advantages of no preservative, good broad-spectrum antibacterial effect, mildness, no irritation, and capability of giving consideration to cleaning, soothing, moisturizing and moisturizing.

Description

Technical Field

[0001] The invention belongs to the field of sanitary products, and relates to baby wet wipes, and in particular to a soothing, moisturizing and mild baby hand and mouth wet wipe that does not contain preservatives. Background Art

[0002] Infants and young children have high water content in their skin, but their skin structure and function are not perfect, so their skin is more prone to dryness and dehydration, and their ability to resist dry environments is weak. As a daily cleaning and care product for infants and young children, wet wipes not only have the task of cleaning, but also need moisturizing function to help babies keep their skin hydrated and prevent dryness and peeling.

[0003] Baby hand and mouth wipes are used to clean the hands or mouth of babies. Since babies have delicate skin and weak resistance, the chemical components left on the skin after wiping with wipes are easy to cause allergies, so baby wipes have higher requirements for care, mildness and safety. At present, most baby hand and mouth wipes on the market are added with approved preservatives (hereinafter referred to as "approved preservatives") specified in the "Technical Specifications for Safety of Cosmetics" (2015 Edition) to avoid microbial contamination and extend the shelf life, such as polyhexamethylene biguanide, chlorhexidine gluconate, parabens, benzyl alcohol, etc. However, these chemical preservatives are common causes of skin allergies and skin irritation in infants and young children. At the same time, their characteristic odor makes infants and young children resist existing wipes. In recent years, the research and use of natural preservatives has been a hot topic. However, natural preservatives also have many limitations in practical applications. Some natural preservatives require high dosages to achieve the preservative effect, which has an adverse effect on the color, odor, stability, etc. of the added products, and even produces toxic side effects.

[0004] Existing baby wet wipes, such as Chinese invention patent CN202311690790.6, disclose a baby wet wipe containing a lauric acid monoglyceride composition and a preparation method. The wet wipes are composed of 72 parts to 92.99 parts of pure water, 1 part to 5 parts of glycerol, 4 parts to 12 parts of a lauric acid monoglyceride composite solution, 1 part to 5 parts of a natural extract and 0.01 part to 1 part of a preservative. During the preparation process, lauric acid monoglyceride is prepared into a composite solution in advance, and natural plant extracts are compounded at the same time. Multiple natural ingredients synergistically enhance the effects of moisturizing, soothing, cleaning and antibacterial. Chinese invention patent CN202311258780.5 discloses a formula for baby wet wipes liquid and a preparation method thereof, which is composed of 0.30% to 0.40% phenoxyethanol, 0.05% to 0.06% chlorphenesin, 0.20% to 0.30% hexamethylene hydrochloride, 0.08% to 0.09% p-hydroxyacetophenone, 0.03% to 0.04% ethylhexylglycerol, 0.05% to 0.06% 1,2-ethylene glycol and 99.29% to 99.05% purified water. It adds preservative and bactericidal ingredients that meet legal requirements, has low irritation to baby skin, and effectively avoids baby skin allergies by adding anti-allergic ingredients and moisturizing ingredients, and can effectively improve the moisturizing effect on baby skin. Chinese invention patent CN201911395600.1 discloses a preservative system for baby wipes and its preparation method. The system components (wt%) include 0.05-0.5% ε-polylysine, 0.08-0.4% lauroyl arginine ethyl ester hydrochloride, 0.01-0.1% natamycin, 0.01-0.05% dehydroacetic acid, 0.01-0.1% lactic acid, 0.1-0.5% alkyl glycoside, 0.2-0.8% glycerol, and 97.55-99.54% secondary reverse osmosis water. It is mild and non-irritating, stable in nature, and has good moisturizing effect. The patent optimizes and compounds ε-polylysine, lauroyl arginine ethyl ester hydrochloride and nano-level natamycin to make an odorless food-grade wet wipes preservative system with a wide spectrum of antiseptic bacteria, high safety performance and strong stability.

[0005] However, most existing wet wipes preservative systems need to be compounded on the basis of approved preservatives, and there are few reports on preservative-free wet wipes preservative systems. Summary of the invention

[0006] In view of the above problems existing in the prior art, the present invention provides a mild, non-irritating, preservative-free, soothing, moisturizing and gentle baby hand and mouth wet wipe that can achieve good broad-spectrum antibacterial effect without adding approved preservatives, and can take into account both cleaning and soothing and moisturizing.

[0007] In order to solve the above technical problems, the present invention adopts the following technical scheme: a soothing, moisturizing and mild baby hand and mouth wet wipes without preservatives, characterized in that: it is composed of a base cloth and a wet wipes liquid, and the wet wipes liquid is composed of the following components in mass percentage: 0.48~0.90% 1,2-hexanediol, 0.15~0.29% p-hydroxyacetophenone, 0.10~0.15% caprylyl glycol, 0.13~0.24% methylpropanediol, 0.04~0.08% ethylhexyl glycol, 5.00% 1,3-butylene glycol, 0.10% disodium EDTA, 0.20~0.50% D-panthenol, 0~0.01% tremella polysaccharide, and the balance is pure water; the base cloth is selected from spunlace non-woven fabric, and the material is one or more of pure cotton or all-viscose or wood pulp composite or plant fiber.

[0008] The present invention also provides a method for preparing a soothing, moisturizing and mild baby hand and mouth wet wipes without preservatives, which is characterized by adopting the following steps: A. Weigh 0.48-0.90% 1,2-hexanediol, 0.15-0.29% p-hydroxyacetophenone, 0.10-0.15% caprylyl glycol, 0.13-0.24% methylpropanediol, 0.04-0.08% ethylhexyl glycol, 5.00% 1,3-butylene glycol, 0.10% disodium EDTA, 0.20-0.50% D-panthenol, 0-0.01% Tremella polysaccharide, and the balance is pure water; B. At room temperature, add 1,2-hexanediol, p-hydroxyacetophenone, caprylyl glycol, methyl propanediol, ethylhexyl glycol, 1,3-butylene glycol, disodium EDTA, D-panthenol, and Tremella polysaccharide weighed in step A to the pure water in sequence, heat to 80-85° C., and stir at a constant speed for 10-20 min until completely dissolved to obtain a wet wipes liquid, wherein the pH value of the wet wipes liquid is 5.0-7.0; C. Cut, fold, arrange, and sterilize the spunlace nonwoven fabric with ultraviolet light, put it into a packaging bag, and then add an appropriate amount of the wet wipes liquid obtained in step B, and then package it to obtain a soothing, moisturizing, and gentle baby hand and mouth wet wipes that does not contain preservatives.

[0009] Furthermore, in the step C, the mass ratio of the wet wipes liquid to the spunlace non-woven fabric in the packaging bag is 3.5:1.

[0010] The present invention combines five ingredients, namely, 1,2-hexanediol, p-hydroxyacetophenone, caprylyl glycol, methylpropanediol and ethylhexylglycerin, in a specific ratio. The combination has a synergistic bactericidal effect and a good broad-spectrum antibacterial effect, so that no permitted preservatives need to be added to the aqueous wet wipes liquid.

[0011] The hydrophilic groups of 1,2-hexanediol and methylpropanediol added in the present invention can capture water molecules, which is helpful for moisturizing, emollient and skin penetration and absorption; at the same time, they can destroy the cell membrane of microorganisms, thus having certain antibacterial properties.

[0012] Caprylyl glycol is a good moisturizer that can moisturize and soften the skin. At the same time, it can improve the antibacterial effect of glycol substances in wet wipes.

[0013] Parahydroxyacetophenone has antioxidant and anti-irritant properties. It affects the proliferation of microorganisms by interfering with the activity of coenzymes that synthesize cell membranes, and has a good preservative and synergistic effect.

[0014] Ethylhexylglycerin is a glyceryl ether with a colorless to light yellow transparent liquid appearance. It has a structure similar to that of a surfactant and can affect the interfacial tension of microbial cell membranes, allowing preservatives to more effectively penetrate into microbial cells and significantly improve the antibacterial effect. Ethylhexylglycerin can be used in combination with 1,2-hexanediol, methylpropanediol, caprylyl glycol, and p-hydroxyacetophenone to produce a synergistic effect, reduce the amount of antibacterial agents added, reduce product toxicity, and reduce irritation. In addition, it can also improve the moisturizing effect of wet wipes, effectively deodorize, and improve skin feel.

[0015] The present invention combines and matches the five ingredients of 1,2-hexanediol, p-hydroxyacetophenone, caprylyl glycol, methylpropanediol and ethylhexylglycerin in a specific ratio, which can effectively control most bacteria and molds, and help to increase the shelf life of the product. At the same time, it combines the antioxidant and soothing properties of the ingredients to enhance skin hydration. The wet wipes are mild in nature and highly safe, and are especially suitable for infants and children and people with sensitive skin. D-panthenol can inhibit the release of inflammatory factors, thereby reducing inflammatory reactions and soothing skin sensitivity. Tremella polysaccharides contain a large number of hydroxyl and carboxyl groups, which can firmly bind to water molecules to form a spatial grid structure, showing a super strong ability to lock in moisture and moisturize the skin, giving the skin a smooth and moisturizing feeling.

[0016] The combined use of active ingredients D-panthenol and Tremella polysaccharide enables the present invention to have the function of soothing and moisturizing.

[0017] In summary, the present invention does not contain any approved preservatives, but has a good broad-spectrum antibacterial effect, is mild and non-irritating, and can take into account the beneficial effects of cleaning, soothing, moisturizing, and moisturizing. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 Schematic diagram of the chick chorioallantoic membrane (CAM) stimulation test results of Examples 1 to 3. DETAILED DESCRIPTION

[0019] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments: Examples 1 to 3 Preparation of preservative-free soothing, moisturizing and mild baby hand and mouth wet wipes Table 1 Wet wipes liquid formula

[0020] Example 1-3 Preservative-free soothing, moisturizing and mild baby hand and mouth wet wipes are prepared by the following method: A. Weigh the raw materials according to the components and mass ratios corresponding to the embodiments in the above formula Table 1, wherein 1,2-hexanediol, p-hydroxyacetophenone, methylpropanediol, and ethylhexylglycerol are Microcare Emollient HG of Thor Specialty Chemicals (Zhenjiang) Co., Ltd., caprylyl glycol is Microcare Emollient CLG of Thor Specialty Chemicals (Zhenjiang) Co., Ltd., and D-panthenol is D-Panthenol of DSM Nutritional Products Co., Ltd.; B. At room temperature, add the raw materials in Table 1 to pure water in sequence, heat to 80-85°C, and stir at a constant speed for 10-20 min until completely dissolved to obtain wet wipes liquid; C. After cutting, folding, sorting, and UV-ozone disinfection, the spunlace nonwoven fabric is put into a packaging bag, and wet wipes liquid is added with a liquid loading volume of 3.5:1 (wet wipes liquid mass: membrane cloth mass). Finally, it is sealed to obtain a soothing, moisturizing, and gentle baby hand and mouth wet wipes that does not contain preservatives.

[0021] Sensory evaluation test of each embodiment: the smell, transparency, uniformity and stability of the wet wipes liquid were evaluated by visual inspection or smell; secondly, actual tests were conducted through some skin sensations of the baby wet wipes during use, and the evaluation results are shown in Table 2.

[0022] Table 2 Sensory evaluation results

[0023] Aging stability test of each embodiment: According to the "Hygiene Standard for Disposable Hygiene Products" GB15979-2002, the soothing, moisturizing and mild baby hand and mouth wipes without preservatives described in Examples 1 to 3 were tested for aging stability. The test data are shown in Table 3.

[0024] Table 3 Aging stability test results of various embodiments

[0025] From the results shown in Table 3, it can be seen that the soothing, moisturizing and mild baby hand and mouth wet wipes without preservatives described in Examples 1 to 3 of the present invention have the characteristics of stable color, no odor, good antiseptic performance, etc.

[0026] Microbial challenge test of each embodiment: Preparation of test bacterial solution: Standard bacterial strains were inoculated and amplified on the culture medium. The bacterial strains were cultured at 36 °C for 24 h. Candida albicans (ATCC 10231) and Aspergillus brasiliensis were cultured in a 28 °C incubator for 48 h and 7 d, respectively. Then, they were added to the elution solution of corresponding concentration for elution, and the number of live bacteria per 1 mL was about 10 8 Cell suspension of standard bacteria.

[0027] Microbial challenge test and evaluation criteria: A 28-day microbial challenge test with one-time addition of bacteria was used. A single test bacterial solution was added to each test sample, and after thorough mixing, samples were taken every 7, 14, and 28 days. The bacterial content of the sample was measured and its logarithmic value was taken for evaluation. The conditions for the sample to pass the preservation challenge test are: the logarithmic value of the number of bacteria at 14 days decreased by no less than 2.0, the number of bacteria from 14 to 28 days did not increase, and the number of fungi did not increase within 14 to 28 days; otherwise, it was deemed to have failed. Refer to the microbial challenge test method of EP. The evaluation criteria are shown in Table 4. The results of the microbial challenge test of Example 1 are shown in Table 5, the results of the microbial challenge test of Example 2 are shown in Table 6, and the results of the microbial challenge test of Example 3 are shown in Table 7.

[0028] Table 4 Microbial challenge evaluation criteria

[0029] Table 5 Microbial challenge test results of Example 1

[0030] Table 6 Microbial challenge test results of Example 2

[0031] Table 7 Microbial challenge test results of Example 3

[0032] According to the test results in Tables 5 to 7, Examples 1 to 3 of the present invention have good inhibitory effects on Pseudomonas aeruginosa, Candida albicans, Staphylococcus aureus and Aspergillus brasiliensis, indicating that in the formula, 1,2-hexanediol, p-hydroxyacetophenone, caprylyl glycol, methylpropylene glycol and ethylhexylglycerol are mixed to synergistically enhance the effect and have obvious antibacterial effect on microorganisms.

[0033] Comparative Examples 1-3 adopt the same preparation method as Example 1-3, except that the wet wipes liquid composition is different, and the wet wipes liquid formula of Comparative Examples 1-3 is shown in Table 8. Caprylyl glycol is not added to the wet wipes formula of Comparative Examples 1-3.

[0034] Table 8 Comparative Example Wet Wipes Liquid Formula

[0035] The microbial challenge test of each comparative example was carried out in the same manner as in Examples 1-3. The microbial challenge test results of Comparative Example 1 are shown in Table 9, the microbial challenge test results of Comparative Example 2 are shown in Table 10, and the microbial challenge test results of Comparative Example 3 are shown in Table 11.

[0036] Table 9 Microbial challenge test results of Comparative Example 1

[0037] Table 10 Microbial challenge test results of Comparative Example 2

[0038] Table 11 Microbial challenge test results of Comparative Example 2

[0039] According to the comparative test results in Tables 9 to 11, it can be seen that Comparative Examples 1 to 3 have good inhibitory effects on Pseudomonas aeruginosa, Candida albicans, and Staphylococcus aureus, but failed the microbial challenge test of mold. It further illustrates that the mixture of 1,2-hexanediol, p-hydroxyacetophenone, caprylyl glycol, methylpropylene glycol, and ethylhexylglycerin can enhance the antiseptic effect.

[0040] Skin irritation test of each embodiment (1) Chicken embryo chorioallantoic membrane (CAM) stimulation test With reference to the reference standard SN / T 2329-2009 "Chicken embryo chorioallantoic membrane test for cosmetic eye irritation / corrosion", SPF eggs were placed in a constant temperature incubator at (37.5±5)℃ and relative humidity of 55%~70% to incubate until 9 days old. The negative control was a 0.9wt% sodium chloride (NaCl) solution; the positive control was a 1.0wt% sodium dodecyl sulfate (SDS) solution.

[0041] Take 300 μL of the sample solution to be tested and drop it on the surface of human CAM to ensure that at least 50% of the CAM surface is covered by the test substance. Immediately after the test substance acts, observe the CAM reaction until 5 minutes, observe the changes in toxic effects, and record the time of vascular bleeding, coagulation, and vascular dissolution effects within 300 seconds of action, and calculate the irritation score (Irritationsoore, IS) and classification. Each treatment is repeated in 6 groups. IS<1 means no irritation; 1≤IS<5 means mild irritation; 5≤IS<9 means moderate irritation; IS>9 means severe irritation. The calculation formula is as follows:

[0042] Where: sec H (bleeding time) - the average time to observe the onset of bleeding on the CAM membrane, in seconds (s); sec L (vascular melting) - the average time to the onset of vascular melting observed on the CAM membrane, in seconds (s); sec C (clotting time) - the average time for the onset of coagulation observed on the CAM membrane, measured in seconds (s).

[0043] The test results of the chicken embryo chorioallantoic membrane irritation test of Example 1 are shown in Table 12, the test results of the chicken embryo chorioallantoic membrane irritation test of Example 2 are shown in Table 13, and the test results of the chicken embryo chorioallantoic membrane irritation test of Example 3 are shown in Table 14.

[0044] Table 12 Test results of chicken embryo chorioallantoic membrane stimulation test of Example 1

[0045] Table 13 Test results of chicken embryo chorioallantoic membrane stimulation test of Example 2

[0046] Table 14 Test results of chicken embryo chorioallantoic membrane stimulation test of Example 3

[0047] Human patch test 0.022±0.001 mL of the wet wipes squeezed liquid was placed in the small chamber of the patch tester; the patch tester with the test substance was applied to the inner side of the subject's arm with a low-allergenic tape; the palm of the hand was gently pressed to make it evenly applied to the skin for 24 hours. Observation was performed 30 minutes (after the indentation disappeared), 24 hours and 48 hours after the removal of the test substance patch tester. According to the "Skin Reaction Grading Standard for Skin Closure Patch Test" in the "Technical Specifications for Safety of Cosmetics (2015 Edition)", see Table 15. The test area was observed and the results were recorded, as shown in Table 16 below. During the test, no positive reactions such as erythema, papules, and edema appeared in the patch area of ​​10 subjects, that is, no adverse irritation reaction occurred in the patch test, which shows that the wet wipes are non-irritating and non-sensitizing to the human body and have good safety. The human patch test results of Examples 1 to 3 are shown in Table 16.

[0048] Table 15 Skin reaction grading standards for occlusive patch test

[0049] Table 16 Human patch test results of Examples 1 to 3

[0050] Moisturizing performance test of each embodiment The test site should not be exposed to any product 2 to 3 days before the test and should not be exposed to water 1 to 3 hours before the test. Before the test, the subjects need to clean the flexor side of both forearms, wipe them gently with a dry paper towel, and sit quietly in the human efficacy evaluation room (temperature 20 to 22 ℃, humidity 40% to 60%) for 30 minutes. During this period, they cannot drink water or beverages. A 9 cm area is planned on the flexor side of the forearm. 2 (3 cm × 3 cm) test area. The inspector used a disposable latex finger cot to wipe the test product on the designated test area, and the blank group was left without any substance. The water content of the stratum corneum of the skin was tested before use of the product and 30 min and 2 h after use. Each area was measured 3 times in parallel each time, and the average value was taken. The test results of the water content of the stratum corneum of the skin of Examples 1-3 are shown in Table 17.

[0051] Table 17 Skin stratum corneum moisture content test results

[0052] The moisturizing effect evaluation is generally characterized by the skin moisture content. The skin moisture content can reflect the moisture state of the skin and indicates the immediate moisturizing effect of the skin. The larger the value, the greater the skin moisture content and the better the immediate moisturizing effect. It can be seen from Table 17 that as time increases, the skin moisture content gradually decreases. The skin moisture content of Examples 1, 2, and 3 is higher than that of the blank group, indicating that the wet wipes have a moisturizing effect.

[0053] To sum up, the above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A soothing, moisturizing and mild preservative-free baby hand and mouth wet wipes, characterized by: The wet wipes liquid is composed of a base fabric and a wet wipes liquid, wherein the wet wipes liquid is composed of the following components in percentage by mass: 0.48-0.90% 1,2-hexanediol, 0.15-0.29% p-hydroxyacetophenone, 0.10-0.15% caprylyl glycol, 0.13-0.24% methylpropanediol, 0.04-0.08% ethylhexyl glycol, 5.00% 1,3-butylene glycol, 0.10% disodium EDTA, 0.20-0.50% D-panthenol, 0-0.01% tremella polysaccharide, and the balance is pure water; the base fabric is selected from spunlace non-woven fabric, and the material is one or more of pure cotton, full viscose, wood pulp composite or plant fiber.

2. The preservative-free soothing, moisturizing and mild baby hand and mouth wet wipes according to claim 1, characterized in that: The mass ratio of the wet wipes liquid to the base fabric is 3.5:

1.

3. A method for preparing a soothing, moisturizing and mild baby hand and mouth wet wipes without preservatives, characterized in that: Use the following steps: A. Weigh 0.48-0.90% 1,2-hexanediol, 0.15-0.29% p-hydroxyacetophenone, 0.10-0.15% caprylyl glycol, 0.13-0.24% methylpropanediol, 0.04-0.08% ethylhexyl glycol, 5.00% 1,3-butylene glycol, 0.10% disodium EDTA, 0.20-0.50% D-panthenol, 0-0.01% Tremella polysaccharide, and the balance is pure water; B. At room temperature, add 1,2-hexanediol, p-hydroxyacetophenone, caprylyl glycol, methyl propanediol, ethylhexyl glycol, 1,3-butylene glycol, disodium EDTA, D-panthenol, and Tremella polysaccharide weighed in step A to the pure water in sequence, heat to 80-85° C., and stir at a constant speed for 10-20 min until completely dissolved to obtain a wet wipes liquid, wherein the pH value of the wet wipes liquid is 5.0-7.0; C. Cut, fold, arrange, and sterilize the spunlace nonwoven fabric with ultraviolet light, put it into a packaging bag, and then add an appropriate amount of the wet wipes liquid obtained in step B, and then package it to obtain a soothing, moisturizing, and gentle baby hand and mouth wet wipes that does not contain preservatives.

4. The method for preparing the preservative-free soothing, moisturizing and mild baby hand and mouth wet wipes according to claim 3, characterized in that: In the step C, the mass ratio of the wet wipes liquid to the spunlace nonwoven fabric in the packaging bag is 3.5:1.

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