Application of urine organic acid in health management

Abstract

The invention discloses an application of urine organic acid in health management. And detecting the urine sample through a liquid chromatography tandem mass spectrometer (LC-MS / MS) to obtain the organic acid value. The urine organic acid value is classified into a first index, a second index, a third index and a fourth index, the detection data comprises an ideal value and a reference value, and if the urine organic acid value is within the ideal value range, the individual health state is evaluated; if the urine organic acid value is within the reference value range, evaluating the sub-health state of the individual; if the value of the organic acid in the urine is beyond the ideal value and the reference value, the individual is evaluated to be in a disease risk state. Therefore, the application of the urine organic acid in health management can achieve the purposes of making up various health management and anti-aging plans for different physiques, and preventing and treating chronic diseases.

Description

Technical Field

[0001] The present invention relates to an application of urine organic acid in health management, and in particular to a method for detecting organic acid in urine to evaluate the health status of the body. The present invention belongs to the field of medical technology. Background Art

[0002] As society progresses, living standards continue to improve, and people enjoy the convenience brought by civilization; however, due to the fast pace of modern life, the body is kept in a high-pressure environment for a long time, which has already caused damage to health. Secondly, as the living environment continues to change, there are many pollutants that harm us around us, which also affect our bodies. In order to avoid more harm to the body, people can only reduce the damage to the living environment as much as possible, and achieve the goal of health preservation by changing their daily eating habits and exercise habits.

[0003] In addition, the global population aging rate is increasing and the birth rate is gradually decreasing, which makes people pay more and more attention to the quality of life and health preservation. Modern people are afraid of the occurrence of diseases, but they ignore that the development of diseases is gradual. The process of the body from health to disease is continuous. When the body function gradually declines, it is also the golden time for us to recover health or stay away from diseases. People need to know whether the body function is changing or where it is changing in a timely manner, so a complete health assessment is needed. Only through the information obtained from the complete function assessment can further improvement and conditioning be carried out.

[0004] In view of the above problems, the inventors have invested a lot of energy and spirit in research and development, and continuously made breakthroughs and innovations in this field, hoping to solve the deficiencies of the existing technology with novel technical means, in addition to bringing better products to the society, and also promoting industrial development. Summary of the invention

[0005] The main purpose of the present invention is to provide an application of a reagent or device for detecting urine organic acids in the preparation of a reagent or device for health management.

[0006] To achieve the above purpose, the present invention adopts the following technical means:

[0007] The present invention proposes the use of a reagent or device for detecting urine organic acids in the preparation of a reagent or device for health management. The reagent or device for detecting urine organic acids is used to detect a urine sample of an individual outside the individual, and the urine sample is detected by a liquid chromatography tandem mass spectrometer to obtain a plurality of urine organic acid values ​​of the urine sample, and the urine organic acid values ​​are classified into a first index, a second index, a third index and a fourth index. Each urine organic acid has an ideal value and a reference value. When the urine organic acid values ​​are within the ideal value range, the individual is evaluated to be in a healthy state; when the urine organic acid values ​​are within the reference value range, the individual is evaluated to be in a sub-healthy state; and when the urine organic acid values ​​are outside the ideal value and the reference value, the individual is evaluated to be in a risk state of suffering from a disease.

[0008] The first index is selected from the group consisting of a fatty acid metabolism index, a carbohydrate metabolism index and a mitochondrial energy production index;

[0009] The second index is selected from a group consisting of vitamin B group index, urea cycle index, oxidative damage DNA and lipid index, antioxidant index and liver detoxification index;

[0010] The third index is selected from the group consisting of a tryptophan metabolism index, a kynurenine metabolism index, a catecholamine metabolism index, and a catecholamine metabolism enzyme activity index;

[0011] The fourth index is selected from the group consisting of an intestinal digestive function index, a Clostridium metabolism index, an intestinal flora overgrowth index, a fungal overgrowth index and an oxalic acid metabolism index.

[0012] In the application, preferably, the fatty acid metabolism index includes an adipic acid value, a suberic acid value and an ethylmalonic acid value; the carbohydrate metabolism index includes a pyruvic acid value, a lactic acid value and a β-hydroxybutyric acid value; and the mitochondrial energy production index includes a citric acid value, a cis-guanosine acid value, an isocitric acid value, an α-ketoglutaric acid value, a succinic acid value, a fumaric acid value, a malic acid value and a hydroxymethylglutaric acid.

[0013] In the application, preferably, the adipic acid value has an ideal value of ≤5.15 μg / mg cr. and a reference value of ≤7.25 μg / mg cr., the suberic acid value has an ideal value of ≤4.58 μg / mg cr. and a reference value of ≤6.47 μg / mg cr., and the ethylmalonic acid value has an ideal value of ≤4.68 μg / mg cr. and a reference value of ≤6.12 μg / mg cr.; the pyruvic acid value has an ideal value of 2.06-5.45 μg / mg cr. and a reference value of 0.23-7.38 μg / mg cr., the lactic acid value has an ideal value of 7.51-24.1 μg / mg cr. and a reference value of 2.8-32.8 μg / mg cr., and the β-hydroxybutyric acid value has an ideal value of ≤2.27 μg / mg cr. and a reference value of ≤3.98 μg / mg cr. reference value; the citric acid value has an ideal value of 241-575 μg / mg cr. and a reference value of 63-748 μg / mg cr., the cis-guanosine value has an ideal value of 49-83.6 μg / mg cr. and a reference value of 29.5-103 μg / mg cr., the isocitric acid value has an ideal value of 58.3-95.9 μg / mg cr. and a reference value of 38.8-116 μg / mg cr., the α-ketoglutaric acid value has an ideal value of 5.5-17.9 μg / mg cr. and a reference value of 0.9-23.7 μg / mg cr., the succinic acid value has an ideal value of ≤10.8 μg / mg cr. and a reference value of ≤18.7 μg / mg cr., the fumaric acid value has an ideal value of ≤0.52 μg / mg cr. and a reference value of ≤0.79 μg / mg cr., the malic acid value has an ideal value of ≤1.34 μg / mg cr. and a reference value of ≤2.00 μg / mg cr., and the hydroxymethylglutaric acid has an ideal value of ≤5.96 μg / mg cr. and a reference value of ≤7.27 μg / mg cr.

[0014] In the application, preferably, the vitamin B group index includes an α-ketoisovaleric acid value, an α-ketoisocaproic acid value, an α-ketoβ-methylvaleric acid value, a xanthopuric acid value, a methylmalonic acid value, an iminomethylglutamic acid value and a β-hydroxyisovaleric acid value; the urea cycle index includes a orotic acid value; the oxidative damage DNA and lipid index includes a deoxyguanosine value and a lipid peroxide value; the antioxidant index includes an α-hydroxybutyric acid value and a pyroglutamic acid value; and the liver detoxification index includes a gluconic acid value.

[0015] In the application, preferably, the α-ketoisovaleric acid value has an ideal value of ≤0.13μg / mg cr. and a reference value of ≤0.2μg / mg cr., the α-ketoisocaproic acid value has an ideal value of ≤0.63μg / mg cr. and a reference value of ≤0.88μg / mgcr., the α-ketoβ-methylvaleric acid value has an ideal value of ≤1.37μg / mg cr. and a reference value of ≤2.13μg / mg cr., the xanthuric acid value has an ideal value of ≤0.49μg / mg cr. and a reference value of ≤0.63μg / mgcr., the methylmalonic acid value has an ideal value of ≤2.27μg / mg cr. and a reference value of ≤2.83μg / mg cr., the iminomethylglutamic acid value has an ideal value of ≤0.28μg / mg cr. and a reference value of ≤0.38μg / mg cr., and the β-hydroxyisovaleric acid value has an ideal value of ≤13.3μg / mg cr. and a reference value of ≤17.0μg / mg cr.; the orotic acid value has an ideal value of ≤0.66μg / mg cr. and a reference value of ≤0.90μg / mg cr.; the deoxyguano ribonucleoside value has an ideal value of ≤4.78μg / mg cr. and a reference value of ≤5.6μg / mg cr., and the lipid peroxide value has an ideal value of ≤7.53μg / mg cr. and a reference value of ≤9.8μg / mg cr.; the α-hydroxybutyric acid value has an ideal value of ≤6.12μg / mg cr. and a reference value of ≤7.37μg / mg cr., and the pyroglutamic acid value has an ideal value of 12-22.1μg / mg cr. and a reference value of 6.76-27.3μg / mg cr. reference value; and the gluconic acid value has an ideal value of ≤8.39μg / mg cr. and a reference value of ≤11.0μg / mg cr.

[0016] In the application, preferably, the tryptophan metabolism index includes a tryptophan value, a 5-hydroxyindoleacetic acid value, a kynurenine value, a kynurenic acid value, a arsenic acid value and a quinolinic acid value; the kynurenine metabolism index includes an IDO enzyme activity value and a KMO enzyme activity value; the catecholamine metabolism index includes a homovanillic acid value, a vanillylmandelic acid value and a dihydroxyphenylacetic acid value; the catecholamine metabolism enzyme activity index includes a DBH enzyme activity value and a COMT enzyme activity value

[0017] In the application, preferably, the tryptophan value has an ideal value of 6.74-17.1 μg / mg cr. and a reference value of 5.39-23.4 μg / mg cr., the 5-hydroxyindole acetic acid value has an ideal value of 4.14-8.10 μg / mg cr. and a reference value of 3.25-10.1 μg / mg cr., the kynurenine value has an ideal value of 0.34-1.11 μg / mg cr. and a reference value of 0.27-1.44 μg / mg cr., the kynurenic acid value has an ideal value of ≤2.84 μg / mg cr. and a reference value of ≤4.86 μg / mg cr., the arsenic acid value has an ideal value of 0.12-0.30 μg / mg cr. and a reference value of 0.072-0.38 μg / mg cr., and the quinolinic acid value has an ideal value of ≤5.93 μg / mg cr. and a reference value of ≤6.96μg / mgcr.; the IDO enzyme activity value has an ideal value of 41.4-90.4μg / mg cr. and a reference value of 34.9-104μg / mgcr. and the KMO enzyme activity value has an ideal value of 1.38-3.22μg / mg cr. and a reference value of 0.85-4.46μg / mg cr.; the homovanillic acid value has an ideal value of 2.81-9.32μg / mg cr. and a reference value of 1.99-10.4μg / mg cr., the vanillylmandelic acid value has an ideal value of 2.89-5.33μg / mg cr. and a reference value of 1.97-5.93μg / mg cr., and the dihydroxyphenylacetic acid value has an ideal value of 0.72-2.14μg / mg cr. and a reference value of 0.54-2.58 μg / mg cr.; the DBH enzyme activity value has an ideal value of 0.87-2.11 μg / mg cr. and a reference value of 0.61-2.84 μg / mg cr., and the COMT enzyme activity value has an ideal value of 2.75-6.28 μg / mg cr. and a reference value of 1.48-7.86 μg / mg cr.

[0018] In the application, preferably, the intestinal digestive function index includes an indoleacetic acid value and a phenylacetic acid value; the Clostridium metabolism index includes a 4-hydroxyphenylacetic acid value, a p-cresol value, and a hydroxypropionic acid value; the intestinal flora overgrowth index includes a dihydroxyphenylpropionic acid value, a hippuric acid value, a p-hydroxybenzoic acid value, and a benzoic acid value; the fungal overgrowth index includes a citramalic acid value, a tartaric acid value, and a triglyceride value; and the oxalate metabolism index includes a glycolate value, a glycerate value, and an oxalate value.

[0019] In the application, preferably, the indoleacetic acid value has an ideal value of ≤37.6μg / mg cr. and a reference value of ≤50.0μg / mg cr., and the phenylacetic acid value has an ideal value of ≤1.70μg / mg cr. and a reference value of ≤2.40μg / mg cr.; the 4-hydroxyphenylacetic acid value has an ideal value of ≤83.0μg / mg cr. and a reference value of ≤99.8μg / mg cr., the p-cresol value has an ideal value of ≤104μg / mg cr. and a reference value of ≤146μg / mg cr., the hydroxypropionic acid value has an ideal value of ≤22.0μg / mg cr. and a reference value of ≤40.2μg / mg cr.; the dihydroxyphenylpropionic acid value has an ideal value of ≤5.45μg / mg cr. and a reference value of ≤6.20μg / mg cr., the hippuric acid value has an ideal value of ≤405μg / mg cr. and a reference value of ≤560μg / mg cr., the p-hydroxybenzoic acid value has an ideal value of ≤5.90μg / mg cr. and a reference value of ≤6.90μg / mg cr., and the benzoic acid value has an ideal value of ≤1.21μg / mg cr. and a reference value of ≤1.95μg / mg cr.; the citramalic acid value has an ideal value of ≤6.65μg / mgcr. and a reference value of ≤7.70μg / mgcr., the tartaric acid value has an ideal value of ≤7.80μg / mg cr. and a reference value of ≤9.61μg / mg cr., the triacylglycerol value has an ideal value of ≤2.31μg / mg cr. and a reference value of ≤2.72μg / mgcr.; the glycolate value has an ideal value of ≤67.0μg / mg cr. and a reference value of ≤83.0μg / mg cr., the glycerate value has an ideal value of ≤5.22μg / mg cr. and a reference value of ≤5.83 μg / mg cr., and the oxalate value has an ideal value of ≤19.1 μg / mg cr. and a reference value of ≤26.4 μg / mg cr.

[0020] In the application, preferably, the device further includes a health management assessment unit, which obtains health promotion knowledge related to the individual's health based on the indicators of the individual to generate health promotion recommendations for the individual. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 The present invention is a flow chart of the detection steps of the application of urine organic acids in health management.

[0022] Explanation of symbols:

[0023] Steps S11 to S15 DETAILED DESCRIPTION

[0024] The following is an explanation of the implementation of the present invention by means of specific embodiments. Those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification. In addition, the present invention can also be implemented or applied by means of other different specific embodiments, and various modifications and changes can be made without departing from the spirit of the present invention.

[0025] See also Figure 1 , Figure 1 The present invention is a flow chart of the detection steps of the application of urine organic acids in health management.

[0026] The present invention proposes an application of a reagent or device for detecting urine organic acids in the preparation of a reagent or device for health management, wherein the reagent or device for detecting urine organic acids detects the urine sample by a liquid chromatography tandem mass spectrometer to obtain the multiple urine organic acid values ​​of the urine sample. Figure 1 As shown, the detection steps of the urine specimen are as follows. As shown in step S11, the urine specimen of the individual is retained overnight, 5 ml of the urine specimen is added to a light-proof tube containing a preservative, and placed in a freezer for storage. As shown in step S12, before the test, the frozen urine specimen is completely thawed, and after centrifugation, 200 μl of the urine specimen is added with 50 μl of the internal standard and 150 μl of secondary pure water. As shown in step S13, after uniform mixing and centrifugation, 40 μl of the urine specimen diluted as above is uniformly mixed for 5 minutes, and then it can be tested on the liquid chromatography tandem mass spectrometer. As shown in step S14, the setting of the liquid chromatography tandem mass spectrometer has been adjusted using a pure standard to determine the collision energy and fragments. The C18 column is used with secondary water and acetonitrile gradient changes for separation. After entering the liquid chromatography tandem mass spectrometer and undergoing two molecular weight screenings, a two-dimensional graph with time as the X-axis and signal intensity as the Y-axis is obtained. The peak area is integrated and corresponds to the peak area of ​​the internal standard. After comparison with the calibration curve, the original concentration is calculated. In addition, the urine sample will have a concentration problem, and the original concentration will be corrected using the creatinine concentration in the urine sample. Creatinine uses automatic biochemical colorimetric analysis.

[0027] The organic acids detected in the urine sample are classified into a first index, a second index, a third index and a fourth index, each urine organic acid value has an ideal value and a reference value, and the urine organic acid values ​​within the ideal value range are evaluated to be in a healthy state; the urine organic acid values ​​within the reference value range are evaluated to be in a sub-healthy state; and the urine organic acid values ​​outside the ideal value and the reference value are evaluated to be in a disease risk state; wherein the first index is selected from fatty acid metabolism index, carbohydrate metabolism index and wherein the second indicator is selected from the group consisting of a vitamin B group indicator, a urea cycle indicator, an oxidative damage DNA and lipid indicator, an antioxidant indicator and a liver detoxification indicator; wherein the third indicator is selected from the group consisting of a tryptophan metabolism indicator, a kynurenine metabolism indicator, a catecholamine metabolism indicator and a catecholamine metabolism enzyme activity indicator; wherein the fourth indicator is selected from the group consisting of an intestinal digestive function indicator, a clostridium metabolism indicator, an intestinal flora overgrowth indicator, a fungal overgrowth indicator and an oxalate metabolism indicator.

[0028] Fatty acid metabolism index

[0029] During the metabolism of fatty acids in the body, carnitine and vitamin B2 work together to bring fatty acids into the mitochondria for β-oxidation and convert them into energy. The fatty acid metabolism index includes an adipic acid value with an ideal value of ≤5.15μg / mg cr. and a reference value of ≤7.5μg / mg cr., a

[0030] A suberic acid value of ≤4.58μg / mg cr. ideal value and ≤6.47μg / mg cr. reference value and an ethylmalonic acid value of ≤4.68μg / mg cr. ideal value and ≤6.12μg / mg cr. reference value. Secondly, the application of urine organic acids in health management of the present invention further includes a health management assessment unit. When carnitine or vitamin B2 in the body is insufficient, acetyl CoA is insufficient, or insulin is too high, the β-oxidation of fatty acids will be hindered, and the adipic acid value, the suberic acid value, and the ethylmalonic acid value are not within the normal range. Therefore, the health management assessment unit can assess the individual's lack of carnitine and vitamin B2 through the fatty acid metabolism index, and recommend the individual to supplement carnitine and vitamin B2.

[0031] Carbohydrate metabolism index

[0032] Under normal physiological conditions, the body can use carbohydrate metabolism as the main energy source. Glycolysis is the reaction of glucose energy metabolism and is also the core of the dynamic balance of pyruvate and lactose in the body. Among them, the carbohydrate metabolism index includes a pyruvate value, a lactate value and a β-hydroxybutyrate value. First, the pyruvate value has an ideal value of 2.06-5.45 μg / mg cr. and a reference value of 0.23-7.38 μg / mg cr. Pyruvate is a normal metabolic product of carbohydrates. When the pyruvate value is low, it indicates a lack of cellular energy-generating substances. Secondly, the lactate value has an ideal value of 7.51-24.1 μg / mg cr. and a reference value of 2.8-32.8 μg / mg cr. When the lactate value is low, it indicates that the materials for making energy from fatty acids, carbohydrates, and proteins are insufficient; high values ​​indicate that the cells are hypoxic or under metabolic stress, which may cause muscle cramps and fatigue. Furthermore, the β-hydroxybutyrate value has an ideal value of ≤2.27μg / mg cr. and a reference value of ≤3.98μg / mg cr. When cells cannot obtain a stable supply of carbohydrates from the diet, they will burn fat as an alternative source of energy, and ketone bodies will be produced at this time.

[0033] Mitochondrial energy production index

[0034] By detecting the intermediate metabolites of the mitochondrial citric acid cycle, the mitochondrial energy production and whether the nutrients or proteins required by the enzymes in the citric acid cycle are deficient can be evaluated; wherein, the mitochondrial energy production index includes a citric acid value, a cis-guanosine value, an isocitric acid value, a α-ketoglutaric acid value, a succinic acid value, a fumaric acid value, a malic acid value and a hydroxymethylglutaric acid value. First, the citric acid value has an ideal value of 241-575 μg / mg cr. and a reference value of 63-748 μg / mg cr., the cis-guanosine value has an ideal value of 49-83.6 μg / mg cr. and a reference value of 29.5-103 μg / mg cr., and the isocitric acid value has an ideal value of 58.3-95.9 μg / mg cr. and a reference value of 38.8-116 μg / mg cr.; wherein, citric acid, cis-guanosine and isocitric acid are involved in the process of energy production and toxic ammonia clearance, when the citric acid value, cis-guanosine and isocitric acid value are too high, it may indicate poor liver detoxification ability and imbalance of intestinal flora; when the citric acid value, cis-guanosine and isocitric acid value are too low, it indicates that it is indirectly caused by abnormal upstream metabolites; amino acid deficiency or indigestion; the health management assessment unit will recommend that the individual supplement glutathione and amino acids. Secondly, the α-ketoglutaric acid value has an ideal value of 5.5 to 17.9 μg / mg cr. and a reference value of 0.9 to 23.7 μg / mg cr.; α-ketoglutaric acid requires vitamins B1, B2, B3, B5 and lipoic acid to be metabolized; when the α-ketoglutaric acid value is too high, it indicates a deficiency of B vitamins and lipoic acid, or an accumulation of toxic metals, and when the α-ketoglutaric acid value is too low, it indicates an amino acid deficiency or indigestion, and the health management assessment unit will recommend that the individual supplement B1, B2, B3, B5, lipoic acid or amino acids. Furthermore, the succinic acid value has an ideal value of ≤10.8μg / mg cr. and a reference value of ≤18.7μg / mg cr., the fumaric acid value has an ideal value of ≤0.52μg / mg cr. and a reference value of ≤0.79μg / mg cr., and the malic acid value has an ideal value of ≤1.34μg / mg cr. and a reference value of ≤2.00μg / mg cr. When the succinic acid value, the fumaric acid value and the malic acid value are high, it indicates that coenzyme Q10 may be deficient, and the health management assessment unit will recommend that the individual supplement CoQ10 or amino acids. In addition, the hydroxymethylglutaric acid value has

[0035] The ideal value is ≤5.96μg / mg cr. and the reference value is ≤7.27μg / mg cr. Hydroxymethylglutaric acid is the raw material used by cells to make coenzyme Q10. When the hydroxymethylglutaric acid value is too high, it indicates that coenzyme Q10 may be deficient. The health management assessment recommends that the individual supplement CoQ10 or amino acids.

[0036] Vitamin B group index

[0037] Vitamin B group is an indispensable nutrient for the cellular nutritional metabolism process and many physiological reactions. When the dietary intake of vitamin B group is insufficient, it will affect mitochondrial function and increase the risk of chronic diseases; among them, the vitamin B group indicators include α-ketoisovaleric acid value, α-ketoisocaproic acid value, α-ketoβ-methylvaleric acid value, xanthuric acid value, methylmalonic acid value, iminomethylglutamate value and β-hydroxyisovaleric acid value. First, the α-ketoisovaleric acid value has an ideal value of ≤0.13μg / mg cr. and a reference value of ≤0.2μg / mg cr., the α-ketoisocaproic acid value has an ideal value of ≤0.63μg / mg cr. and a reference value of ≤0.88μg / mg cr., and the α-ketoβ-methylvaleric acid value has an ideal value of ≤1.37μg / mg cr. and a reference value of ≤2.13μg / mg cr.; B1, B2, B3, B5 and lipoic acid are coenzymes of α-ketoisovaleric acid, α-ketoisocaproic acid and α-ketoβ-methylvaleric acid metabolic enzymes. When B1, B2, B3, B5 and lipoic acid are lacking, they cannot be metabolized normally, making the α-ketoisovaleric acid value, the α-ketoisocaproic acid value and the α-ketoβ-methylvaleric acid value high, which may also be related to toxic metals and hyperdiabetes. Secondly, the xanthuric acid value has an ideal value of ≤0.49μg / mg cr. and a reference value of ≤0.63μg / mg cr. When the xanthuric acid value is high, it indicates that vitamin B6 is insufficient, and the health management assessment unit will recommend that the individual supplement B6. Furthermore, the methylmalonic acid value has an ideal value of ≤2.27μg / mg cr. and a reference value of ≤2.83μg / mg cr. When the methylmalonic acid value is high, it indicates that vitamin B12 is insufficient, so the high methylmalonic acid value may also be related to the low GSH affecting the conversion of vitamin B12, and the health management assessment unit will recommend that the individual supplement B12. In addition, the iminomethylglutamate value has an ideal value of ≤0.28μg / mg cr. and a reference value of ≤0.38μg / mg cr. When the iminomethylglutamate value is high, it indicates that folic acid is insufficient or metabolic abnormalities, which may be impaired methylation and / or high homocysteine. In addition, the β-hydroxyisovaleric acid value has an ideal value of ≤13.3μg / mg cr. and a reference value of ≤17.0μg / mg cr. When the value is high, it indicates biotin deficiency. The health management assessment will recommend that the individual supplement biotin.

[0038] Urea cycle indicators

[0039] When the urea cycle metabolism is abnormal, excess ammonia in the body will be converted into orotic acid, which may be related to drug-induced liver toxicity, viral hepatitis, and inborn errors of metabolism. Excessive ammonia can affect the brain and cause symptoms such as headaches, poor concentration, and anxiety. The urea cycle index includes an ideal value of ≤0.66μg / mg cr. and

[0040] ≤0.90μg / mg cr. reference value of orotic acid value. If the orotic acid value is too high, and the citric acid value, the cis-aconitic acid value and the isocitric acid value are too high, it indicates poor ammonia elimination ability. The health management assessment unit will recommend that the individual supplement with arginine or ornithine aspartate.

[0041] Oxidative damage to DNA and lipid markers

[0042] The byproduct of mitochondrial energy metabolism is free radicals. When the body's antioxidant system is insufficient to cope with and remove free radicals, it will undergo oxidation reactions with structures such as cell DNA, lipids or proteins, producing more oxidative substances and increasing oxidative stress. Studies have found that excessive oxidative stress is associated with many disease risks, such as cardiovascular metabolic diseases, autoimmune diseases, cancer, etc. The oxidative damage to DNA and lipid indicators include a deoxyguano ribonucleoside value and a lipid peroxide value. First, the deoxyguano ribonucleoside value has an ideal value of ≤4.78μg / mg cr. and a reference value of ≤5.6μg / mg cr. Deoxyguano ribonucleoside is a free radical in the body that attacks DNA. The deoxyguano ribonucleoside value can detect the situation of oxidative damage to cellular DNA in the body. The health management assessment will recommend that the individual supplement antioxidant nutrients. Secondly, the lipid peroxide value has an ideal value of ≤7.53μg / mg cr. and a reference value of ≤9.8μg / mg cr. Lipid peroxide is a peroxide produced by free radicals attacking lipids in the body. Excessive LPO will cause cell damage and induce immune inflammatory response. The individual will be advised to supplement antioxidant nutrients.

[0043] Antioxidant index and liver detoxification index

[0044] Glutathione (GSH) is one of the strongest antioxidants in the body. When the body is under high oxidative stress, the body will compensate by increasing glutathione synthesis. Among them, the antioxidant index includes α-hydroxybutyric acid value and pyroglutamic acid value. First, the α-hydroxybutyric acid value has an ideal value of ≤6.12μg / mg cr. and

[0045] ≤7.37μg / mg cr. reference value, α-hydroxybutyric acid is an indicator of liver glutathione synthesis rate, and is also an early indicator of insulin resistance and glucose intolerance. Secondly, the pyroglutamate value has an ideal value of 12-22.1μg / mg cr. and a reference value of 6.76-27.3μg / mg cr. Pyroglutamate is an indicator of kidney and small intestine amino acid recovery. When the pyroglutamate value is high, it means that the body has exhausted glutathione (GSH) to prevent the loss of other amino acids; when the pyroglutamate value is low, it means that glutamate intake is insufficient or precursors are lacking, toxic load, and facing oxidative stress. The health management assessment unit will recommend that the individual supplement glutathione.

[0046] Liver detoxification index

[0047] When there is too much accumulation of toxins in cells, it may damage the function of cell mitochondria, such as intestinal flora metabolites, prescription drugs, pro-inflammatory foods, etc. Therefore, the liver detoxification index can be used to understand the degree of body detoxification load; among them, the liver detoxification index includes a gluconic acid value with an ideal value of ≤8.39μg / mg cr. and a reference value of ≤11.0μg / mg cr. Gluconic acid is required for the liver detoxification process, so it can be used as an indicator of liver detoxification function. When the liver's detoxification load is large, or the liver's second stage detoxification function is disordered, the liver detoxification index will increase, and the health management assessment unit will recommend that the individual supplement with calcium glucarate or antioxidant nutrients.

[0048] Tryptophan metabolism index

[0049] Tryptophan is a precursor of serotonin. It has the function of assisting intestinal mucosal regeneration and inhibiting potential pathogens through IL-22. It also provides raw materials for enterochromaffin cells to synthesize serotonin. When tryptophan is metabolized in the liver, it will be converted into quinolinic acid in the presence of sufficient vitamin B6 and metabolized to the pathway of synthesizing NAD. Among them, the tryptophan metabolism index includes tryptophan value, 5-hydroxyindoleacetic acid value, kynurenine value, kynurenic acid value, arsenic acid value and quinolinic acid value. First, the tryptophan value has an ideal value of 6.74~17.1μg / mg cr. and a reference value of 5.39~23.4μg / mg cr. When intestinal tryptophan is insufficient, it will affect the function of the digestive tract and is related to inflammatory bowel disease (IBD) and irritable bowel syndrome (IBS). When the tryptophan value and other urine amino acids such as phenylalanine and tyrosine increase at the same time, it means that tetrahydrobiopterin (BH4) and vitamin B6 are insufficient; low values ​​may be related to insufficient protein intake, or may be due to intestinal mucosal inflammation and changes in intestinal flora. Secondly, the 5-hydroxyindole acetate value has an ideal value of 4.14-8.10μg / mg cr. and a reference value of 3.25-10.1μg / mg cr. 5-hydroxyindole acetate (5-HIAA) is a metabolite of serotonin and can reflect the concentration of serotonin in the body. If the 5-hydroxyindole acetate value is abnormally elevated, it is recommended to exclude intestinal neuroendocrine tumors; when the 5-hydroxyindole acetate value is low, it indicates insufficient serotonin, which may be related to insufficient precursor tryptophan, or insufficient cofactor BH4 or vitamin B6. Common symptoms include constipation, depression, fatigue and inattention. The health management assessment unit will recommend supplementing vitamin B6 or tryptophan. Furthermore, the kynurenine value has an ideal value of 0.34-1.11 μg / mg cr. and a reference value of 0.27-1.44 μg / mg cr. Tryptophan is metabolized into kynurenine by the TDO enzyme in the liver, and the inflammatory response stimulates the IDO enzyme to increase the synthesis of kynurenine. In addition, the kynurenic acid value has an ideal value of ≤2.84 μg / mg cr. and a reference value of ≤4.86 μg / mg cr. Kynurenic acid has a neuroprotective effect and can be used as an NMDA receptor antagonist to resist inflammation, protect nerves from oxidative stress attacks, and reduce pain. When the kynurenic acid value is high, it may be due to insufficient expression of the KMO gene or damage to the enzyme. Studies have shown that the overactivation of the pathway for synthesizing kynurenic acid (NMDA antagonist) in astrocytes is related to schizophrenia. In addition, the kynurenine value has an ideal value of 0.12-0.30 μg / mg cr. and a reference value of 0.072-0.38 μg / mg cr. Kynurenine is one of the metabolites of kynurenine in the process of forming NAD in the liver, and it activates Th1-related inflammatory cytokines in the immune system.Next, the quinolinic acid value has an ideal value of ≤5.93μg / mg cr. and a reference value of ≤6.96μg / mg cr. During an inflammatory response, macrophages stimulate microglia to synthesize quinolinic acid, which has neurotoxicity to the brain and reduces mitochondrial activity.

[0050] Kynurenine metabolism index

[0051] The kynurenine metabolic pathway is closely related to inflammatory responses, immune systems, and neurological diseases. Under inflammatory responses, macrophages increase the synthesis of kynurenine through the IDO enzyme. Astrocytes in the brain convert kynurenine into kynurenic acid, which has a neuroprotective effect and can act as an NMDA receptor antagonist. It has anti-inflammatory, nerve protection from oxidative stress attacks, and pain reduction functions. Among them, the kynurenine metabolic index includes the IDO enzyme activity value and the KMO enzyme activity value. First, the IDO enzyme activity value has an ideal value of 41.4 to 90.4 μg / mg cr. and a reference value of 34.9 to 104 μg / mg cr. The enzyme activity of IDO is increased by the catalysis of proinflammatory cytokines such as IFN-α, -β, -γ, TNF-α, IL-6, and PAF, prompting macrophages to synthesize kynurenine from tryptophan. Secondly, the KMO enzyme activity value has an ideal value of 1.38-3.22μg / mg cr. and a reference value of 0.85-4.46μg / mg cr. IFN-γ stimulates microglia and macrophages to synthesize quinolinic acid through Th1 reaction. Quinolinic acid stimulates NMDA receptors, inhibits antioxidant enzyme activity, and reduces mitochondrial activity. Studies have found that the concentration of cortical kynurenic acid in the brain and cerebrospinal fluid of patients with schizophrenia is increased, which may be due to insufficient expression of the KMO gene or damage to the enzyme. The health management assessment unit will recommend supplementation of SPMs, Or Omega-3.

[0052] Catecholamine metabolism markers

[0053] The metabolites of dopamine, norepinephrine and adrenaline can show the body's response to stress, which is mainly related to internal emotional stress or exogenous environmental toxins. Whether the amino acids phenylalanine and tyrosine, the precursors of catecholamine synthesis, are sufficient, or the intestinal flora affects the activity of enzymes that metabolize neurotransmitters; among them, the catecholamine metabolism index includes homovanillic acid value, vanillylmandelic acid value and dihydroxyphenylacetic acid value. First, the homovanillic acid value has an ideal value of 2.81-9.32μg / mg cr. and a reference value of 1.99-10.4μg / mg cr. Homovanillic acid is a metabolite of the neurotransmitter dopamine, which shows the reaction of the nervous system through dopamine. When the homovanillic acid value is high, it means that you are in a state of stress or excitement, which accelerates the metabolism of dopamine. Secondly, the vanillylmandelic acid value has an ideal value of 2.89-5.33 μg / mg cr. and a reference value of 1.97-5.93 μg / mg cr. Vanillylmandelic acid is a metabolite of norepinephrine and adrenaline, indicating the reaction of the nervous system through norepinephrine and adrenaline. Furthermore, the dihydroxyphenylacetic acid value has an ideal value of 0.72-2.14 μg / mg cr. and a reference value of 0.54-2.58 μg / mg cr. Dihydroxyphenylacetic acid is another major metabolite of dopamine, indicating whether dopamine synthesis is normal or whether the synthesis of neurotransmitters is affected by exogenous environmental toxins. The low dihydroxyphenylacetic acid value may be related to the deficiency of amino acids, the precursors of dopamine synthesis. The health management assessment unit will recommend the use of MAO inhibitory drugs, spore probiotics or tyrosine supplementation.

[0054] Catecholamine metabolic enzyme activity index

[0055] Intestinal Clostridium metabolites, mold fusaric acid metabolites, disulfiram drugs, food additives aspartame, etc. will inhibit the activity of dopamine β-hydroxylase (DBH) enzyme, and COMT enzyme represents the ability of methylation, which is related to the balance of neurotransmitters; among them, the catecholamine metabolism enzyme activity index includes DBH enzyme activity value and COMT enzyme activity value. First, the DBH enzyme activity value has an ideal value of 0.87-2.11μg / mg cr. and a reference value of 0.61-2.84μg / mg cr. DBH enzyme activity represents the activity of dopamine β-hydroxylase (DBH), an enzyme that converts dopamine into norepinephrine. A high DBH enzyme activity value indicates insufficient DBH activity, usually due to intestinal Clostridium metabolites inhibiting the activity of enzymes such as HPHA, 4-cresol and 4-HPAA. The health management assessment unit will recommend supplementing spore probiotics. Secondly, the COMT enzyme activity value has an ideal value of 2.75-6.28 μg / mg cr. and a reference value of 1.48-7.86 μg / mg cr. The COMT enzyme activity value is the HVA / DOPAC ratio. HVA and DOPAC are both major metabolites of dopamine. The HVA / DOPAC ratio can show the COMT enzyme activity and methylation ability.

[0056] Intestinal digestive function index

[0057] Polyphenols, tyrosine, phenylalanine or tryptophan that are not absorbed in the intestine will be decomposed by bacteria, which can reflect problems with poor protein digestion, insufficient gastric acid or imbalance of intestinal flora; among them, the intestinal digestive function index includes an indoleacetic acid value and a phenylacetic acid value. First, the indoleacetic acid value has an ideal value of ≤37.6μg / mg cr. and a reference value of ≤50.0μg / mg cr. Indoleacetic acid is a metabolite of tryptophan that is not absorbed by the intestine and is decomposed by bacteria, and is related to the overgrowth of Clostridia in the intestine. The high indoleacetic acid value indicates poor tryptophan absorption, which may be related to insufficient gastric acid, imbalance of intestinal flora, and overgrowth of Clostridia. The health management assessment unit will recommend supplementing with digestive enzymes or spore probiotics. Secondly, the phenylacetic acid value has an ideal value of ≤1.70μg / mg cr. and a reference value of ≤2.40μg / mgcr. A high phenylacetic acid value indicates poor absorption of tyrosine or phenylalanine, low gastric acid, and imbalance of intestinal flora. The health management assessment unit will recommend supplementation with digestive enzymes or spore probiotics.

[0058] Clostridium metabolic index

[0059] The intestinal flora metabolites of tryptophan, tyrosine, and phenylalanine are particularly related to the overgrowth of Clostridia; wherein the Clostridia metabolic index includes 4-hydroxyphenylacetic acid value, p-cresol value, and hydroxypropionic acid value. First, the 4-hydroxyphenylacetic acid value has an ideal value of ≤83.0μg / mg cr. and a reference value of ≤99.8μg / mg cr. 4-hydroxyphenylacetic acid is a metabolite of tyrosine that is not absorbed by the intestine and is decomposed by bacteria, and is related to the overgrowth of Clostridia in the intestine. The high 4-hydroxyphenylacetic acid value is related to Giardia lamblia infection, abuse of antibiotics to promote the proliferation of protozoa and anaerobic bacteria, overgrowth of Clostridia, abnormal proliferation of small intestinal bacteria, or small intestinal diseases. Secondly, the p-cresol value has an ideal value of ≤104μg / mg cr. and a reference value of ≤146μg / mg cr. p-cresol is a metabolite of phenylalanine that is not absorbed by the intestine and is decomposed by bacteria, and is related to the overgrowth of Clostridium in the intestine. The high p-cresol value indicates the overgrowth of Clostridium difficile (C.diffcile) in the intestine, and the toxins secreted may cause mild to moderate diarrhea and pseudomembranous colitis. Furthermore, the hydroxypropionic acid value has an ideal value of ≤22.0μg / mg cr. and a reference value of ≤40.2μg / mg cr. hydroxypropionic acid is a metabolite of phenylalanine that is not absorbed by the intestine and is decomposed by bacteria, and is related to the overgrowth of Clostridium in the intestine. The high hydroxypropionic acid value indicates the overgrowth of Clostridium and botulinum, secreting neurotoxins such as botulinum toxin, and the related symptoms are weakness of the limbs and chest muscles, impaired vision, fatigue and difficulty in speaking, and botulism may come from contaminated food. The health management assessment unit will recommend supplementation with spore probiotics.

[0060] Indicators of intestinal flora overgrowth

[0061] Overgrowth of intestinal flora will decompose amino acids, chlorogenic acid, and polyphenols that are not fully absorbed by the intestine, resulting in an increase in metabolites. The use of antibiotics will affect the balance of intestinal flora. Frequent contact with preservatives in cosmetics, jams, and pie fillings will increase the amount of parabens in the body, or pickled vegetables, ham, and sausages contain benzoic acid as a preservative, which will cause such indicators to rise; among them, indicators of intestinal flora overgrowth include dihydroxyphenylpropionic acid values, hippuric acid values, parahydroxybenzoic acid values, and benzoic acid values. First, the dihydroxyphenylpropionic acid value has an ideal value of ≤5.45μg / mg cr. and a reference value of ≤6.20μg / mg cr. Dihydroxyphenylpropionic acid is a metabolite of phenylalanine that is not fully absorbed, or chlorogenic acid in beverages, fruits, and vegetables that is decomposed by bacteria. A high dihydroxyphenylpropionic acid value indicates the proliferation of Clostridium, Lactobacillus, Bifidobacterium, and Escherichia coli. Secondly, the hippuric acid value has an ideal value of ≤405μg / mg cr. and a reference value of ≤6.20μg / mg cr.

[0062] ≤560μg / mg cr. reference value. Hippuric acid is a metabolite of phenylalanine that is not absorbed by the intestine and is decomposed by bacteria. It is also a combination of benzoic acid and glycine. When the intestinal flora overgrows, the bacteria will decompose the polyphenols in beverages, vegetables, and fruits, especially chlorogenic acid, and the hippuric acid value will increase. The health management assessment unit will recommend supplementing spore probiotics. Furthermore, the parahydroxybenzoic acid value has an ideal value of ≤5.90μg / mg cr. and a reference value of ≤6.90μg / mg cr. Parahydroxybenzoic acid is a bacterial metabolite of tyrosine. Antibiotics will promote the growth of such bacteria. Due to cosmetics, the body contains too much parahydroxybenzoic acid esters, which causes the parahydroxybenzoic acid value to increase. The high parahydroxybenzoic acid value indicates that the body contains too many preservative parahydroxybenzoic acid esters, intestinal flora imbalance, celiac disease or gluten sensitivity, enteritis or intestinal resection. The health management assessment unit will recommend supplementing spore probiotics, glycine or vitamin B5. In addition, the benzoic acid value has an ideal value of ≤1.21μg / mgcr. and a reference value of ≤1.95μg / mgcr. Benzoic acid is naturally present in fruits such as berries, and is commonly found in pickled foods and canned meats. It may also come from the preservative benzoic acid and is related to small intestinal flora overgrowth (SIBO), cystic fibrosis, and celiac disease. When the hippuric acid index is normal and the benzoic acid value is elevated, it may be related to poor glycine binding in the second stage of liver detoxification. The health management assessment unit will recommend supplementing with spore probiotics, glycine, or vitamin B5.

[0063] Fungal overgrowth indicators

[0064] Fungi generally refer to yeast, Candida or Fusarium. When they grow excessively in the body, they will increase the inflammatory response and may be related to autoimmune diseases. Among them, the fungal overgrowth index includes citramalic acid value, tartaric acid value and propionic acid value. First, the citramalic acid value has an ideal value of ≤6.65μg / mg cr. and a reference value of ≤7.70μg / mgcr. Citramalic acid is a metabolite of yeast, anaerobic bacteria Propionibacterium acnes or Clostridium. The high citramalic acid value indicates excessive growth of yeast or anaerobic bacteria, and the health management assessment unit will recommend supplementing Saccharomyces boulardii. Secondly, the tartaric acid value has an ideal value of ≤7.80μg / mg cr. and a reference value of ≤9.61μg / mg cr. Tartaric acid is a product of Candida using hyaluronidase to decompose hyaluronic acid (hyaluronic acid), and is also present in foods containing tartaric acid. The high tartaric acid value indicates a large intake of foods containing tartaric acid (fruits), or tartar powder used in some carbonated beverages and baked goods. In addition, the triacylglycerol value has an ideal value of ≤2.31μg / mg cr. and a reference value of ≤2.72μg / mg cr. Triacylglycerol is a metabolite of fumonisin produced by Fusarium that is released through the intestine and will bind tightly to minerals, affecting mineral absorption.

[0065] Oxalate metabolism index

[0066] In addition to being associated with kidney stones, excessive oxalate crystals can also form in bones, joints, blood vessels, thyroid glands, or even in the brain. The sharp structure of oxalate crystals can cause pain and damage to various tissues, increase inflammatory responses, and affect the normal function of organs. Oxalate accumulation in the brain and vascular tissues can cause oxidative damage, and the accumulation of oxalate mercury crystals in tissues may also be one of the causes of autism. The high correlation between arabinose and oxalate, a metabolite of fungal hyperplasia, indicates that fungal overgrowth is likely to be one of the main reasons for the increase in oxalate in autism groups; among them, the oxalate metabolism index includes glycolate value, glycerate value, and oxalate value. First, the glycolate value has an ideal value of ≤67.0μg / mgcr. and a reference value of ≤83.0μg / mg cr. In the case of genetic disease type 1 hyperoxaluria (Hyperoxaluria Type 1) and vitamin B6 deficiency, the enzyme activity of alanine glyoxylate aminotransferase is insufficient, resulting in glyoxylate being converted to glycolate via other enzymes. When glycolic acid increases, it may indicate primary hyperoxaluria or vitamin B6 deficiency. Secondly, the glycerate value has an ideal value of ≤5.22μg / mg cr. and a reference value of ≤5.83μg / mg cr. The genetic disease Hyperoxaluria Type 2 lacks the GRHPR enzyme, so glyoxylic acid will increase the conversion to oxalate. If oxalate and glycerate increase at the same time, it may indicate hyperoxaluria Type 2. Furthermore, the oxalate value has an ideal value of ≤19.1μg / mg cr. and a reference value of ≤5.83μg / mg cr.

[0067] ≤26.4μg / mg cr. Reference value. In addition to being related to kidney stones, excessive oxalate is also associated with primary hyperoxaluria, autism, fibromyalgia, and female vulvar pain. The elevated oxalate value may be due to exogenous high doses of vitamin C, environmental antifreeze agent ethylene glycol, and arabinose, a metabolite of excessive growth of Aspergillus, Penicillium, or Candida in the body. The health management assessment unit will recommend supplementation with vitamin B6 or Saccharomyces boulardii.

[0068] However, the above description is only a preferred embodiment of the present invention and is not intended to limit the patent protection scope of the present invention. Therefore, all equivalent changes made using the contents of the present invention's specification and drawings are also included in the scope of protection of the present invention and are hereby stated.

Claims

1. Use of a reagent or device for detecting organic acids in urine in the preparation of a reagent or device for health management, characterized in that: The reagent or device for detecting urine organic acids is used to detect a urine sample of an individual outside a body, and the urine sample is detected by a liquid chromatography tandem mass spectrometer to obtain a plurality of urine organic acid values ​​of the urine sample, and the urine organic acid values ​​are classified into a first index, a second index, a third index and a fourth index. Each urine organic acid has an ideal value and a reference value. When the urine organic acid values ​​are within the ideal value range, the individual is evaluated to be in a healthy state; when the urine organic acid values ​​are within the reference value range, the individual is evaluated to be in a sub-healthy state; and when the urine organic acid values ​​are outside the ideal value and the reference value, the individual is evaluated to be in a risk state of suffering from a disease. The first index is selected from the group consisting of a fatty acid metabolism index, a carbohydrate metabolism index and a mitochondrial energy production index; The second index is selected from a group consisting of vitamin B group index, urea cycle index, oxidative damage DNA and lipid index, antioxidant index and liver detoxification index; The third index is selected from the group consisting of a tryptophan metabolism index, a kynurenine metabolism index, a catecholamine metabolism index, and a catecholamine metabolism enzyme activity index; The fourth index is selected from the group consisting of an intestinal digestive function index, a Clostridium metabolism index, an intestinal flora overgrowth index, a fungal overgrowth index and an oxalic acid metabolism index.

2. The use according to claim 1, characterized in that The fatty acid metabolism index includes an adipic acid value, a suberic acid value and an ethylmalonic acid value; the carbohydrate metabolism index includes a pyruvic acid value, a lactic acid value and a β-hydroxybutyric acid value; and the mitochondrial energy production index includes a citric acid value, a cis-guanosine acid value, an isocitric acid value, an α-ketoglutaric acid value, a succinic acid value, a fumaric acid value, a malic acid value and a hydroxymethylglutaric acid.

3. The use according to claim 2, characterized in that The adipic acid value has an ideal value of ≤5.15 μg / mg cr. and a reference value of ≤7.25 μg / mg cr., the suberic acid value has an ideal value of ≤4.58 μg / mg cr. and a reference value of ≤6.47 μg / mg cr., and the ethylmalonic acid value has an ideal value of ≤4.68 μg / mg cr. and a reference value of ≤6.12 μg / mg cr.; the pyruvic acid value has an ideal value of 2.06-5.45 μg / mg cr. and a reference value of 0.23-7.38 μg / mg cr., the lactic acid value has an ideal value of 7.51-24.1 μg / mg cr. and a reference value of 2.8-32.8 μg / mg cr., and the β-hydroxybutyric acid value has an ideal value of ≤2.27 μg / mg cr. and a reference value of ≤3.98 μg / mg cr. reference value; the citric acid value has an ideal value of 241-575 μg / mg cr. and a reference value of 63-748 μg / mg cr., the cis-guanosine value has an ideal value of 49-83.6 μg / mg cr. and a reference value of 29.5-103 μg / mg cr., the isocitric acid value has an ideal value of 58.3-95.9 μg / mg cr. and a reference value of 38.8-116 μg / mg cr., the α-ketoglutaric acid value has an ideal value of 5.5-17.9 μg / mg cr. and a reference value of 0.9-23.7 μg / mg cr., the succinic acid value has an ideal value of ≤10.8 μg / mg cr. and a reference value of ≤18.7 μg / mg cr., the fumaric acid value has an ideal value of ≤0.52μg / mgcr. and a reference value of ≤0.79μg / mgcr., the malic acid value has an ideal value of ≤1.34μg / mg cr. and a reference value of ≤2.00μg / mg cr., and the hydroxymethylglutaric acid has an ideal value of ≤5.96μg / mg cr. and a reference value of ≤7.27μg / mg cr.

4. The use according to claim 1, characterized in that The vitamin B group index includes an α-ketoisovaleric acid value, an α-ketoisocaproic acid value, an α-ketoβ-methylvaleric acid value, a xanthopuric acid value, a methylmalonic acid value, an iminomethylglutamic acid value and a β-hydroxyisovaleric acid value; the urea cycle index includes an orotic acid value; the oxidative damage DNA and lipid index includes a deoxyguanosine value and a lipid peroxide value; the antioxidant index includes an α-hydroxybutyric acid value and a pyroglutamic acid value; and the liver detoxification index includes a gluconic acid value.

5. The use according to claim 4, characterized in that The α-ketoisovaleric acid value has an ideal value of ≤0.13 μg / mg cr. and a reference value of ≤0.2 μg / mg cr., the α-ketoisocaproic acid value has an ideal value of ≤0.63 μg / mg cr. and a reference value of ≤0.88 μg / mg cr., the α-ketoβ-methylvaleric acid value has an ideal value of ≤1.37 μg / mg cr. and a reference value of ≤2.13 μg / mg cr., the xanthuric acid value has an ideal value of ≤0.49 μg / mg cr. and a reference value of ≤0.63 μg / mg cr., the methylmalonic acid value has an ideal value of ≤2.27 μg / mg cr. and a reference value of ≤2.83 μg / mg cr., the iminomethylglutamic acid value has an ideal value of ≤0.28 μg / mg cr. and a reference value of ≤0.38 μg / mg cr., and the β-hydroxyisovaleric acid value has an ideal value of ≤13.3 μg / mg cr. and a reference value of ≤17.0μg / mg cr.; the orotic acid value has an ideal value of ≤0.66μg / mg cr. and a reference value of ≤0.90μg / mgcr.; the deoxyguano ribonucleoside value has an ideal value of ≤4.78μg / mg cr. and a reference value of ≤5.6μg / mgcr., and the lipid peroxide value has an ideal value of ≤7.53μg / mg cr. and a reference value of ≤9.8μg / mg cr.; the α-hydroxybutyric acid value has an ideal value of ≤6.12μg / mgcr. and a reference value of ≤7.37μg / mg cr., and the pyroglutamic acid value has an ideal value of 12-22.1μg / mg cr. and a reference value of 6.76-27.3μg / mg cr.; and the gluconic acid value has an ideal value of ≤8.39μg / mg cr. and a reference value of ≤11.0μg / mg cr.

6. The use according to claim 1, characterized in that The tryptophan metabolism index includes a tryptophan value, a 5-hydroxyindoleacetic acid value, a kynurenine value, a kynurenine acid value, a arsenic acid value and a quinolinic acid value; the kynurenine metabolism index includes an IDO enzyme activity value and a KMO enzyme activity value; the catecholamine metabolism index includes a homovanillic acid value, a vanillylmandelic acid value and a dihydroxyphenylacetic acid value; the catecholamine metabolism enzyme activity index includes a DBH enzyme activity value and a COMT enzyme activity value.

7. The use according to claim 6, characterized in that The tryptophan value has an ideal value of 6.74-17.1 μg / mg cr. and a reference value of 5.39-23.4 μg / mg cr., the 5-hydroxyindole acetic acid value has an ideal value of 4.14-8.10 μg / mg cr. and a reference value of 3.25-10.1 μg / mg cr., the kynurenine value has an ideal value of 0.34-1.11 μg / mg cr. and a reference value of 0.27-1.44 μg / mg cr., the kynurenic acid value has an ideal value of ≤2.84 μg / mg cr. and a reference value of ≤4.86 μg / mg cr., the arsenic acid value has an ideal value of 0.12-0.30 μg / mg cr. and a reference value of 0.072-0.38 μg / mg cr., and the quinolinic acid value has an ideal value of ≤5.93 μg / mg cr. and a reference value of ≤6.96μg / mg cr.; the IDO enzyme activity value has an ideal value of 41.4-90.4μg / mg cr. and a reference value of 34.9-104μg / mg cr. and the KMO enzyme activity value has an ideal value of 1.38-3.22μg / mg cr. and a reference value of 0.85-4.46μg / mg cr.; the homovanillic acid value has an ideal value of 2.81-9.32μg / mg cr. and a reference value of 1.99-10.4μg / mg cr., the vanillylmandelic acid value has an ideal value of 2.89-5.33μg / mg cr. and a reference value of 1.97-5.93μg / mg cr., and the dihydroxyphenylacetic acid value has an ideal value of 0.72-2.14μg / mg cr. and a reference value of 0.54-2.58μg / mg cr. reference value; the DBH enzyme activity value has an ideal value of 0.87-2.11 μg / mg cr. and a reference value of 0.61-2.84 μg / mg cr., and the COMT enzyme activity value has an ideal value of 2.75-6.28 μg / mg cr. and a reference value of 1.48-7.86 μg / mg cr.

8. The use according to claim 1, characterized in that The intestinal digestive function index includes an indoleacetic acid value and a phenylacetic acid value; the Clostridium metabolism index includes a 4-hydroxyphenylacetic acid value, a p-cresol value, and a hydroxypropionic acid value; the intestinal flora overgrowth index includes a dihydroxyphenylpropionic acid value, a hippuric acid value, a p-hydroxybenzoic acid value, and a benzoic acid value; the fungal overgrowth index includes a citramalic acid value, a tartaric acid value, and a triglyceride value; and the oxalic acid metabolism index includes a glycolate value, a glycerate value, and an oxalate value.

9. The use according to claim 8, characterized in that The indoleacetic acid value has an ideal value of ≤37.6μg / mgcr. and a reference value of ≤50.0μg / mg cr., and the phenylacetic acid value has an ideal value of ≤1.70μg / mg cr. and a reference value of ≤2.40μg / mg cr.; the 4-hydroxyphenylacetic acid value has an ideal value of ≤83.0μg / mg cr. and a reference value of ≤99.8μg / mg cr., the p-cresol value has an ideal value of ≤104μg / mgcr. and a reference value of ≤146μg / mgcr., the hydroxypropionic acid value has an ideal value of ≤22.0μg / mg cr. and a reference value of ≤40.2μg / mg cr.; the dihydroxyphenylpropionic acid value has an ideal value of ≤5.45μg / mg cr. and a reference value of ≤6.20μg / mg cr., the hippuric acid value has an ideal value of ≤405μg / mg cr. and a reference value of ≤560μg / mg cr., the ideal value of the para-hydroxybenzoic acid value is ≤5.90μg / mg cr. and the reference value is ≤6.90μg / mg cr., and the ideal value of the benzoic acid value is ≤1.21μg / mg cr. and the reference value is ≤1.95μg / mg cr.; the ideal value of the citramalic acid value is ≤6.65μg / mg cr. and the reference value is ≤7.70μg / mgcr., the ideal value of the tartaric acid value is ≤7.80μg / mg cr. and the reference value is ≤9.61μg / mg cr., the ideal value of the triglyceride value is ≤2.31μg / mg cr. and the reference value is ≤2.72μg / mg cr.; the ideal value of the glycolate value is ≤67.0μg / mg cr. and the reference value is ≤83.0μg / mg cr., the ideal value of the glycerate value is ≤5.22μg / mg cr. and the reference value is ≤5.83μg / mg cr., and the oxalate value has an ideal value of ≤19.1 μg / mg cr. and a reference value of ≤26.4 μg / mg cr.

10. The use according to claim 1, characterized in that The device further includes a health management assessment unit, which obtains health promotion knowledge related to the individual's health according to the indicators of the individual to generate health promotion suggestions for the individual.

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