Traditional Chinese medicine composition for preventing and treating chicken coccidiosis and preparation method thereof

Abstract

The invention belongs to the field of veterinary traditional Chinese medicines, and provides a traditional Chinese medicine composition for preventing and treating chicken coccidiosis and a preparation method thereof, the traditional Chinese medicine composition comprises cortex meliae, radix stemonae, betel nut, felwort, herba andrographitis, houttuynia cordata, cyrtomium fortunei, elecampane, szechwan chinaberry fruit, immature bitter orange, fructus ulmi, semen raphani and rice sprout. Experiments prove that the traditional Chinese medicine composition can significantly improve the relative weight gain rate and survival rate of chickens, improve the lesion degree of cecum and reduce the number of oocysts in excrement, and has good treatment and prevention effects on chicken coccidiosis. The traditional Chinese medicine composition disclosed by the invention has the advantages of long acting time, high safety, difficulty in generating drug resistance and very high development value.

Description

Technical Field

[0001] The invention relates to the field of veterinary Chinese medicines, and in particular to a Chinese medicine composition for preventing and treating chicken coccidiosis, and a preparation method and application thereof. Background Art

[0002] Chicken coccidiosis is commonly known as "bloody diarrhea". Chicken coccidia is a parasite distributed worldwide. Among the seven species of Eimeria recognized worldwide, Eimeria tenella is the most toxic and harmful. The damage caused by coccidia mainly includes the following aspects: first, to meet their own needs, coccidia directly absorb nutrients from the host's digestive tract, causing the host's feed utilization rate to decrease and become emaciated; second, coccidia directly parasitize in the host's intestinal epithelium, causing cell damage, leading to intestinal bleeding and inflammation; third, the metabolites produced by coccidia can cause oxidative stress and immune response in the body, destroy intestinal homeostasis, lead to secondary infection, and thus induce systemic pathological changes.

[0003] Eimeria coccidiosis can cause intestinal mucosal barrier damage, digestive dysfunction, and nutrient absorption disorders. It is a common disease in animal farming (especially poultry, ruminants, and rabbits), causing huge economic losses. In recent years, a variety of broad-spectrum anticoccidial drugs have been used to prevent and treat Eimeria coccidiosis, which has brought about serious coccidial resistance and food safety issues. At present, resistant strains of commonly used anticoccidial drugs have appeared, and even multi-drug resistant strains have appeared. Vaccination is one of the effective measures to prevent chicken coccidiosis, with advantages such as no residue and significant protective effect. However, coccidiosis vaccines have limitations in terms of safety and operability, which makes it urgent to find new methods and new drugs that can effectively prevent and treat coccidiosis.

[0004] Chicken coccidiosis is a type of intestinal damp-heat parasite-producing bloody diarrhea, and treatment should be to clear away heat and dampness, kill parasites and stop diarrhea. It is currently believed that the anti-coccidiosis effect of traditional Chinese medicine is, on the one hand, to inhibit the development of coccidia, and on the other hand, to relieve the pathological conditions caused by coccidia infection in the intestine by regulating the immune function of the animal body. Traditional Chinese medicine has a variety of chemical drugs with a single active ingredient, which have more diverse functions and make up for the shortcomings of chemical drugs that require the use of several drugs due to their single effect. The use of traditional Chinese medicine compositions can not only directly reach the lesions and expel coccidia, but also regulate the spleen and stomach, promote feed absorption, and further enhance the body's immunity and promote body recovery. Summary of the invention

[0005] In order to solve the above technical problems, the present invention provides a Chinese medicine composition for preventing and treating chicken coccidiosis, the Chinese medicine composition for preventing and treating chicken coccidiosis comprises Melia azedarach bark, Stemona tuber, Areca nut, Gentiana scabra, Andrographis paniculata, Houttuynia cordata, Rhizoma Cynomorii, Aucklandia lappa, Toosendan fruit, Fructus Aurantii Immaturus, Radish seed, and Malus bud. The Chinese medicine composition of the present invention can directly reach the lesion, expel coccidiosis, has a good therapeutic and preventive effect on chicken coccidiosis, regulates the spleen and stomach, enhances the body's immunity, further promotes the body's recovery, and has high safety and does not produce drug resistance.

[0006] In certain embodiments, the Chinese medicine composition comprises, by weight:

[0007] 40-60 parts of Chinaberry bark, 20-40 parts of Stemona, 35-55 parts of Areca, 20-40 parts of Gentiana, 20-40 parts of Andrographis, 10-30 parts of Houttuynia, 20-40 parts of Cyperus, 8-25 parts of Aucklandia, 10-28 parts of Toosendan, 10-28 parts of Citrus aurantium, 5-25 parts of Radish, 20-40 parts of Radish Seed, and 5-25 parts of Malus.

[0008] In certain embodiments, the Chinese medicine composition comprises, by weight:

[0009] 45-55 parts of Chinaberry bark, 25-35 parts of Stemona, 40-50 parts of Areca, 25-35 parts of Gentiana, 25-35 parts of Andrographis, 15-25 parts of Houttuynia, 25-35 parts of Cyperus, 10-20 parts of Aucklandia, 12-24 parts of Toosendan fruit, 12-24 parts of Citrus aurantium, 10-20 parts of Radish, 25-35 parts of Radish seed, and 10-20 parts of malt sprout.

[0010] In one embodiment, the Chinese medicine composition comprises, by weight:

[0011] 50 parts of Chinese neem bark, 30 parts of stemona, 45 parts of areca nut, 30 parts of gentian, 30 parts of andrographis paniculata, 20 parts of houttuynia, 20 parts of osmanthus, 15 parts of costusroot, 18 parts of toosendan fruit, 18 parts of immature bitter orange, 15 parts of radish seeds, 20 parts of radish seeds, and 15 parts of malt sprout.

[0012] The present invention also provides a method for preparing the Chinese medicinal composition for preventing and treating chicken coccidiosis, comprising the following steps:

[0013] (1) Crush the material through a 40-60 mesh sieve;

[0014] (2) Add 10-15 times the amount of water, reflux and extract at 100°C for 2-4 times, each time for 1-3 hours, filter, and combine the filtrates;

[0015] (3) lowering the temperature of the filtrate to 37-42° C., adding cellulase and ligninase, adjusting the pH to 5.0, and performing ultrasound-assisted enzymatic extraction at an ultrasonic frequency of 30-40 kHz for 55-65 min to obtain an enzyme extract; and boiling for 8 min to inactivate the enzyme;

[0016] (4) The enzyme extract is concentrated under vacuum to an extract density of 1.05-1.15, spray-dried, and pulverized to prepare a conventional dosage form.

[0017] In certain embodiments, the mass ratio of the cellulase to the ligninase is 2-3:1.

[0018] In certain embodiments, the dosage form is a powder, a premix, a soluble powder, a granule, an oral solution or a tablet.

[0019] The present invention also provides application of the Chinese medicinal composition for preventing and treating chicken coccidiosis in preparing medicine for preventing and treating chicken coccidiosis.

[0020] The present invention also provides application of the Chinese medicinal composition for preventing and treating chicken coccidiosis in preparing feed for preventing and treating chicken coccidiosis.

[0021] The invention also provides feed containing the Chinese medicinal composition for preventing and treating chicken coccidiosis.

[0022] The Chinese medicine prescription of the present invention is guided by the Chinese medicine veterinary theory and follows the compatibility principle. In the prescription, bark of chinaberry, stemonae and areca are the monarch drugs, gentian, herba andrographis, herba houttuyniae and rhizoma cyrtomii are the minister drugs, and the monarch and minister drugs are combined to enhance the insecticidal effect. Radix aucklandiae, fructus toosendan, immature bitter orange, radish seeds and malt sprout are the assistant drugs, which play the role of harmonizing the drug properties, increasing the drug stability and promoting absorption in the prescription. The prescription of the present invention is reasonable in compatibility, and the monarch, minister, assistant and assistant drugs are used together, which play the effect of killing insects and eliminating accumulation, clearing away heat and drying dampness, and strengthening the spleen and stomach, can expel coccidia, enhance immunity, promote body recovery and improve the condition of sick chickens.

[0023] Compared with the prior art, the present invention has the following advantages:

[0024] (1) The Chinese medicine composition of the present invention has been verified by experiments to significantly improve the relative weight gain rate and survival rate of chickens, improve the degree of cecal lesions, reduce the number of oocysts in feces, and has a good therapeutic and preventive effect on chicken coccidiosis.

[0025] (2) The Chinese medicine composition of the present invention can not only directly reach the lesion and expel coccidia, but also regulate the spleen and stomach, promote feed absorption, further enhance the body's immunity, and promote the body's recovery.

[0026] (3) The Chinese medicine composition of the present invention is prepared from natural Chinese herbal medicines, has the advantages of long-lasting efficacy, no residue, little irritation to animals, high safety, and will not cause drug resistance, and has high potential development value. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 The present invention is the test result of the Chinese medicine composition for treating chicken coccidiosis. DETAILED DESCRIPTION

[0028] In order to verify the efficacy of the Chinese medicine composition of the present invention, the inventors conducted a pharmacodynamics test study. It should be noted that the drugs selected for the pharmacodynamics test study of the present invention are drugs obtained by the representative formula of the present invention and its preparation method. Due to space limitations, the tests and results involved in the drugs obtained by other formulas and preparation methods included in the present invention are not listed here one by one.

[0029] Example 1: A Chinese medicine composition for preventing and treating chicken coccidiosis, the dosage and preparation method are as follows:

[0030] 50 parts of Chinese neem bark, 30 parts of stemona, 45 parts of areca nut, 30 parts of gentian, 30 parts of andrographis paniculata, 20 parts of houttuynia, 20 parts of osmanthus, 15 parts of costusroot, 18 parts of toosendan fruit, 18 parts of immature bitter orange, 15 parts of radish seeds, 20 parts of radish seeds, and 15 parts of malt sprout.

[0031] The preparation method is as follows:

[0032] (1) Grind the material through a 50-mesh sieve;

[0033] (2) Add 15 times the amount of water, reflux and extract at 100°C for 3 times, 2 h each time, filter, and combine the filtrates;

[0034] (3) lowering the temperature of the filtrate to 39°C, adding 0.16% cellulase and 0.08% ligninase at 40°C, adjusting the pH to 5.0, and performing ultrasound-assisted enzymatic extraction at an ultrasonic frequency of 35 kHz for 60 min to obtain an enzyme extract; boiling for 8 min to inactivate the enzyme;

[0035] (4) The enzyme extract is concentrated under vacuum to an extract density of 1.10, spray-dried, and pulverized to prepare a powder.

[0036] Example 2: A Chinese medicine composition for preventing and treating chicken coccidiosis, the dosage and preparation method are as follows:

[0037] 45 parts of Chinese neem bark, 35 parts of stemona, 40 parts of areca nut, 35 parts of gentian, 25 parts of andrographis, 25 parts of houttuynia, 25 parts of osmanthus, 10 parts of costusroot, 24 parts of toosendan fruit, 24 parts of immature bitter orange, 10 parts of radish seeds, 35 parts of radish seeds, and 10 parts of malt sprout.

[0038] The preparation method is as follows:

[0039] (1) Grind the material through a 40-mesh sieve;

[0040] (2) Add 15 times the amount of water, reflux and extract at 100°C twice, each time for 3 h, filter, and combine the filtrates;

[0041] (3) The filtrate temperature was lowered to 37° C., 0.15% cellulase and 0.05% ligninase were added, the pH was adjusted to 5.0, and the ultrasonic frequency was 40 kHz for 65 min of ultrasound-assisted enzyme extraction to obtain an enzyme extract; the enzyme was inactivated by boiling for 8 min;

[0042] (4) The enzyme extract is concentrated under vacuum to an extract density of 1.05, spray-dried, and crushed to prepare granules.

[0043] Example 3: A Chinese medicine composition for preventing and treating chicken coccidiosis, the dosage and preparation method are as follows:

[0044] 55 parts of Chinese neem bark, 25 parts of stemonae, 50 parts of areca nut, 25 parts of gentian, 35 parts of andrographis paniculata, 15 parts of houttuynia cordata, 35 parts of osmanthus fragrans, 20 parts of costusroot, 18 parts of toosendan fruit, 12 parts of immature bitter orange, 10 parts of radish seed, 25 parts of radish seed, 20 parts of malt sprout.

[0045] The preparation method is as follows:

[0046] (1) Grind the material through a 60-mesh sieve;

[0047] (2) Add 10 times the amount of water, reflux and extract at 100°C for 4 times, each time for 1 h, filter, and combine the filtrates;

[0048] (3) The filtrate temperature was lowered to 42° C., 0.21% cellulase and 0.07% ligninase were added, the pH was adjusted to 5.0, and the ultrasonic frequency was 30 kHz for 55 min of ultrasound-assisted enzyme extraction to obtain an enzyme extract; the enzyme was inactivated by boiling for 8 min;

[0049] (4) The enzyme extract is concentrated under vacuum to an extract density of 1.15, spray-dried, and crushed to prepare tablets.

[0050] Example 4: A Chinese medicine composition for preventing and treating chicken coccidiosis, the dosage and preparation method are as follows:

[0051] 52 parts of Chinese neem bark, 33 parts of stemona, 49 parts of areca catechu, 32 parts of gentian, 27 parts of andrographis paniculata, 23 parts of houttuynia cordata, 15 parts of cyrtomium, 20 parts of costusroot, 16 parts of toosendan fruit, 19 parts of immature bitter orange, 3 parts of radish seed, 23 parts of radish seed, and 18 parts of malt sprout.

[0052] The preparation method is as follows:

[0053] (1) Crush the material and pass it through a 55-mesh sieve;

[0054] (2) Add 10 times the amount of water, reflux and extract at 100°C for 4 times, each time for 2 h, filter, and combine the filtrates;

[0055] (3) The filtrate temperature was lowered to 40° C., 0.16% cellulase and 0.08% ligninase were added, the pH was adjusted to 5.0, and the ultrasonic frequency was 350 kHz for 60 min of ultrasound-assisted enzyme extraction to obtain an enzyme extract; the enzyme was inactivated by boiling for 8 min;

[0056] (4) The enzyme extract is concentrated under vacuum to an extract density of 1.12, spray-dried, and pulverized to prepare a soluble powder.

[0057] Example 5: A Chinese medicine composition for preventing and treating chicken coccidiosis, the dosage and preparation method are as follows:

[0058] 60 parts of Chinese neem bark, 20 parts of stemona, 35 parts of areca nut, 20 parts of gentian, 40 parts of andrographis, 30 parts of houttuynia, 40 parts of osmanthus, 8 parts of costusroot, 10 parts of toosendan fruit, 28 parts of immature bitter orange, 5 parts of radish, 40 parts of radish seeds, and 25 parts of malt sprout.

[0059] The preparation method is the same as Example 1.

[0060] Example 6: A Chinese medicine composition for preventing and treating chicken coccidiosis, the dosage and preparation method are as follows:

[0061] 40 parts of Chinese neem bark, 40 parts of stemonae, 55 parts of areca nut, 40 parts of gentian, 20 parts of andrographis paniculata, 10 parts of houttuynia cordata, 20 parts of cyrtomium, 25 parts of costusroot, 28 parts of toosendan fruit, 10 parts of immature bitter orange, 25 parts of radish seed, 20 parts of radish seed, 5 parts of malt sprout.

[0062] The preparation method is the same as Example 1.

[0063] Comparative Example 1: A Chinese medicine composition for preventing and treating chicken coccidiosis, the dosage and preparation method are as follows:

[0064] 50 parts of Artemisia annua, 30 parts of Sophora flavescens, 45 parts of Radix Dichroae, 30 parts of Gentiana scabra, 30 parts of Andrographis paniculata, 20 parts of Houttuynia cordata, 20 parts of Cynomorium macrostemon, 15 parts of Aucklandia lappa, 18 parts of Toosendan fruit, 18 parts of Citrus aurantium, 15 parts of Radish seed, 20 parts of Radish seed, and 15 parts of Gastrodia elata.

[0065] The preparation method is the same as Example 1.

[0066] Comparative Example 2: A Chinese medicine composition for preventing and treating chicken coccidiosis, the dosage and preparation method are as follows:

[0067] 50 parts of Chinese neem bark, 30 parts of stemona, 45 parts of areca catechu, 30 parts of phellodendron, 30 parts of qinpi, 20 parts of forsythia, 20 parts of dandelion, 15 parts of costusroot, 18 parts of toosendan fruit, 18 parts of immature bitter orange, 15 parts of radish seed, 20 parts of radish seed, and 15 parts of malt sprout.

[0068] The preparation method is the same as Example 1.

[0069] Comparative Example 3: A Chinese medicine composition for preventing and treating chicken coccidiosis, the dosage and preparation method are as follows:

[0070] 50 parts of Chinese neem bark, 30 parts of stemona, 45 parts of areca nut, 30 parts of gentian, 30 parts of andrographis paniculata, 20 parts of houttuynia, 20 parts of osmanthus, 15 parts of citron, 18 parts of green peel, 18 parts of persimmon calyx, 15 parts of sedge, 20 parts of radish seeds, and 15 parts of malt sprout.

[0071] The preparation method is the same as Example 1.

[0072] Comparative Example 4: A Chinese medicine composition for preventing and treating chicken coccidiosis, the dosage and preparation method are as follows:

[0073] 50 parts of Chinese neem bark, 30 parts of stemona, 45 parts of areca nut, 30 parts of gentian, 30 parts of andrographis paniculata, 20 parts of houttuynia, 20 parts of osmanthus, 15 parts of costusroot, 18 parts of toosendan fruit, 18 parts of immature bitter orange, 15 parts of schizonepeta tenuifolia, 20 parts of malt, and 15 parts of Shenqu.

[0074] The preparation method is the same as Example 1.

[0075] Comparative Example 5: A Chinese medicine composition for preventing and treating chicken coccidiosis, the dosage and preparation method are as follows:

[0076] 65 parts of Chinese neem bark, 45 parts of stemonae, 25 parts of areca nut, 15 parts of gentian, 35 parts of andrographis paniculata, 8 parts of houttuynia cordata, 15 parts of osmanthus fragrans, 30 parts of costusroot, 8 parts of toosendan fruit, 30 parts of immature bitter orange, 15 parts of radish seeds, 15 parts of malt sprout.

[0077] The preparation method is the same as Example 1.

[0078] Comparative Example 6: A Chinese medicine composition for preventing and treating chicken coccidiosis, the dosage and preparation method are as follows:

[0079] During the preparation process, 0.15% cellulase and 0.10% ligninase were added, and other conditions were the same as in Example 1.

[0080] Effect Example 1 Experimental study on the therapeutic effect of the Chinese medicine composition of the present invention on chicken coccidiosis

[0081] 1 Experimental methods

[0082] 1.1 Experimental animals

[0083] 300 one-day-old three-yellow chickens were selected from *** breeding farm and fed until 15 days old.

[0084] 1.2 Grouping and treatment of experimental animals

[0085] Three hundred healthy three-yellow chickens aged 15 days were randomly divided into Example 1 group, Example 2 group, Example 3 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, Comparative Example 4 group, Comparative Example 5 group, model group and blank group, with 30 chickens in each group. Except for the blank group, the remaining groups of experimental chickens were orally administered 5×10 3 The sporulated oocysts were orally administered once to artificially replicate the chicken coccidiosis model. The blank group was orally administered with the same volume of saline. The number of fecal oocysts was detected on the 4th day after the administration of the eggs. When the number of oocysts per gram of feces was 1×10 4 ~1×10 5 The drug was administered when the chickens showed clinical symptoms such as poor spirit, loss of appetite, messy and dull feathers, and clumping.

[0086] Example 1 group: Chinese medicinal composition of Example 1 + basic diet (0.5 g of the Chinese medicinal composition of Example 1 was added to each kilogram of basic diet);

[0087] Example 2 group: Chinese medicinal composition of Example 2 + basic diet (0.5 g of the Chinese medicinal composition of Example 2 was added to each kilogram of basic diet);

[0088] Example 3 group: Chinese medicinal composition of Example 3 + basic diet (0.5 g of the Chinese medicinal composition of Example 3 was added to each kilogram of basic diet);

[0089] Comparative Example 1 group: Chinese medicinal composition of Comparative Example 1 + basic diet (0.5 g of the Chinese medicinal composition of Comparative Example 1 was added to each kilogram of basic diet);

[0090] Comparative Example 2 group: Chinese medicinal composition of Comparative Example 2 + basic diet (0.5 g of Chinese medicinal composition of Comparative Example 2 was added to each kilogram of basic diet);

[0091] Comparative Example 3 group: Chinese medicinal composition of Comparative Example 3 + basic diet (0.5 g of Chinese medicinal composition of Comparative Example 3 was added to each kilogram of basic diet);

[0092] Comparative Example 4 group: Chinese medicinal composition of Comparative Example 4 + basic diet (0.5 g of the Chinese medicinal composition of Comparative Example 4 was added to each kilogram of basic diet);

[0093] Comparative Example 5 group: Chinese medicinal composition of Comparative Example 5 + basic diet (0.5 g of the Chinese medicinal composition of Comparative Example 5 was added to each kilogram of basic diet);

[0094] Model group: basal diet;

[0095] Blank group: basic diet.

[0096] All experimental chickens were fed in the same environment, kept warm by heat lamps, immunized normally, and fed the same feed without any drug additives. The experimental chickens were free to drink water and eat.

[0097] 1.3 Indicator Observation

[0098] During the experiment, the mental state, feeding, drinking, exercise and feces of each group of experimental chickens were observed and recorded.

[0099] 1.4 Determination of average weight gain and relative weight gain rate

[0100] All experimental chickens were weighed before and at the end of the experiment, and the average weight gain and relative weight gain rate of each group of experimental chickens were calculated. Average weight gain = average final weight - average initial weight; relative weight gain rate = average weight gain of chickens in each group / average weight gain of chickens in the blank group × 100%.

[0101] 1.5 Survival rate

[0102] During the test, observe and record the death of each group of experimental chickens due to coccidia infection, and calculate the survival rate. Survival rate = number of surviving chickens in the group / total number of chickens in the group before the test × 100%.

[0103] 1.6 Scoring of cecal lesions

[0104] At the end of the experiment, all the experimental chickens were dissected and scored according to the lesion scoring criteria.

[0105] No macroscopic lesions were recorded as 0 points; there were a few scattered petechiae on the cecal wall, no thickening, and normal cecal contents, which was recorded as 1 point; there were a large number of lesions, the cecal contents were obviously bloody, and the cecal wall was slightly thickened, which was recorded as 2 points; there was a lot of blood and intestinal core in the cecum, a large number of blood clots or gray-white cheesy lumps were visible, the cecal wall was obviously thickened, and the amount of feces in the cecum was small, which was recorded as 3 points; the cecum was filled with a large amount of blood and intestinal core, the cecum was swollen, and there was fecal residue in the intestinal core; chickens died of coccidia infection, which was recorded as 4 points.

[0106] When scoring, if the cecal lesions on both sides are inconsistent, the more serious side will be used for judgment. Death due to coccidiosis will be scored as 4 points. The lesion value of each group will be calculated based on the scoring results. The lesion value = average lesion score of each group × 10.

[0107] 1.7 Fecal coccidia counts

[0108] Before administration and at the end of the experiment, the feces of the experimental chickens in each group were collected to count the coccidia oocysts. The oocyst ratio = the oocyst discharge amount of each group / the oocyst discharge amount of the model group × 100%, and the oocyst value was calculated according to Table 1.

[0109] Table 1 Oocyst ratio and oocyst value

[0110]

[0111] 1.8 Determination of the anticoccidial index (ACI)

[0112] The therapeutic effect of the drug was comprehensively evaluated by measuring the oocyst value, relative weight gain rate, lesion value and survival rate of each group of test chickens. ACI = (relative weight gain rate + survival rate) - (lesion value + oocyst value). The efficacy judgment standard is: ACI < 120, poor efficacy; 120 ≤ ACI < 160, moderate efficacy; 160 ≤ ACI < 180, good efficacy; ACI ≥ 180, excellent efficacy.

[0113] 1.9 Statistical analysis

[0114] SPSS 22.0 software was used for data processing. The data were expressed as mean ± standard deviation. The data of each group were tested for normal distribution and homogeneity of variance, which met the requirements of Pearson's test, and one-way analysis of variance was used for comparison between groups. P < 0.05 was considered statistically significant.

[0115] 2. Experimental results

[0116] 2.1 Effect of the Chinese medicine composition of the present invention on the relative weight gain rate of experimental chickens

[0117] Table 2 Effect of the Chinese medicine composition of the present invention on the relative weight gain rate of experimental chickens

[0118]

[0119] Note: Compared with the blank group, * P<0.05, ** P < 0.01;

[0120] Compared with the model group, & P<0.05, && P < 0.01;

[0121] Comparative Example 1 group comparison, @ P<0.05, @@ P<0.01.

[0122] As shown in Table 2, after infection with coccidia, the average weight gain of the chicks in the model group was significantly lower than that in the normal group, indicating that the growth of the chicks was inhibited after infection with coccidia. The average weight gain of the chicks in Example 1, Example 2, and Example 3 groups was significantly higher than that in the model group, indicating that the Chinese medicine composition in Example 1, Example 2, and Example 3 groups in the diet can alleviate the growth inhibition of the chicks after coccidia infection. The average weight gain of the chicks in Example 1, Example 2, and Example 3 groups was significantly higher than that in Comparative Example 1, indicating that the effect of relieving the growth inhibition of the chicks after coccidia infection in Example 1, Example 2, and Example 3 groups was better than that in Comparative Example 1.

[0123] Table 3 Effect of the Chinese medicine composition of the present invention on the survival rate of experimental chickens

[0124]

[0125] As shown in Table 3, 15 chickens died in the model group, and no chicks died in Example 1, Example 2, and Example 3. 7, 10, 9, 6, and 7 chickens died in Comparative Example 1, Comparative Example 2, Comparative Example 3, Comparative Example 4, and Comparative Example 5, respectively.

[0126] Table 4 Effect of the Chinese medicine composition of the present invention on the relative weight gain rate of experimental chickens

[0127]

[0128] As shown in Table 4, typical cecal tissue lesions occurred in chicks infected with coccidia: the cecum became thicker, the intestinal wall thickened, there were scattered bleeding spots, blood clots or white lumps in the cecal contents, and in severe cases, there were bloody intestinal cores; while the cecal lesions in Example 1-3 groups were alleviated.

[0129] After administration, compared with the model group, the cecal lesion scores of the chickens in Example 1, Example 2 and Example 3 groups were significantly reduced, and the cecal damage was significantly alleviated.

[0130] Compared with the model group, the cecal lesion scores of the experimental chickens in Comparative Example Group 1, Comparative Example Group 2, Comparative Example Group 3, Comparative Example Group 4, and Comparative Example Group 5 were reduced, but the effect was weaker than that of Example 1-3 groups.

[0131] Table 5 Effect of the Chinese medicine composition of the present invention on the relative weight gain rate of experimental chickens

[0132]

[0133] The number of eggs per gram of feces can directly reflect the reproductive capacity of oocysts and is an important indicator for evaluating the severity of coccidia infection in chickens. As shown in Table 5, after administration, the oocyst values ​​of the experimental chickens in Example 1, Example 2, and Example 3 groups were significantly reduced compared with the model group.

[0134] Compared with the model group, the oocyst values ​​of the experimental chickens in Comparative Example Group 1, Comparative Example Group 2, Comparative Example Group 3, Comparative Example Group 4, and Comparative Example Group 5 were reduced, but the effect was weaker than that of Example 1-3 groups.

[0135] Table 6 Effect of the Chinese medicine composition of the present invention on ACI of experimental chickens

[0136]

[0137]

[0138] ACI is the most commonly used and most reliable indicator for evaluating the therapeutic effect of drugs. The results of this study show that, as shown in Table 6, after the Chinese medicine composition of Example 1, Example 2, and Example 3 was mixed with feed and fed, the ACI of each group was greater than 180, and the efficacy was evaluated as excellent. The ACI of Comparative Example 1, Comparative Example 2, Comparative Example 3, Comparative Example 4, and Comparative Example 5 were all less than 120.

[0139] The Chinese medicinal composition of the invention has good therapeutic and preventive effects on artificial infection of chickens with Eimeria tenella.

[0140] Effect Example 2 Clinical Effect Verification of the Chinese Medicine Composition of the Present Invention on Chicken Coccidiosis

[0141] 600 sick chickens suffering from coccidiosis were selected from a broiler farm in Shandong Province, and were treated with the drug of the present invention and the comparative drug respectively. The selected sick chickens were listless, huddled together and lethargic, their feed intake was significantly reduced, and bloody stools were discharged. Due to problems such as drug resistance, the use effect of diclazuril solution was not good.

[0142] 600 sick chickens were randomly divided into 3 groups, each with 200 chickens, which were respectively the drug groups prepared by Example 1 of the present invention, Comparative Example 1, and Comparative Example 6. The Example 1 group and the Comparative Example 1 and Comparative Example 6 groups were all fed with 0.5 g of the Chinese medicine composition per kilogram of the basic diet for 7 consecutive days, and other feeding environments remained the same.

[0143] Criteria for judging the therapeutic effect: (1) Effective: The spirit and appetite of the sick chickens return to normal, bloody stools disappear, the number of oocysts in the feces decreases significantly and there is no recurrence; (2) Ineffective: After medication, the symptoms of the sick chickens are not relieved, the number of oocysts in the feces does not decrease, there is still bloody stool and there is a possibility of worsening or death. The test results are shown in Tables 7, 8 and Figure 1 .

[0144] Table 7 Test results

[0145]

[0146] Table 8 Test results

[0147]

[0148] It can be seen from the test results in Tables 7 and 8 that the Chinese medicine composition of Example 1 of the present invention has a significant therapeutic effect on chicken coccidia, with an effective rate of 97%, while the effective rates of the Chinese medicine compositions of Comparative Examples 1 and 6 are lower than 90%.

[0149] The above embodiments are not any form of limitation to the present invention. For those skilled in the art, any modification, replacement, etc. made within the scope of the technical solution of the present invention based on the technical essence of the present invention still falls within the protection scope of the technical solution of the present invention.

Claims

1. A Chinese medicine composition for preventing and treating chicken coccidiosis, characterized in that: The Chinese medicine composition comprises bark of Melia azedarach, stemona, areca nut, gentian, andrographis paniculata, houttuynia cordata, cyperus rotundus, costusroot, toosendan fruit, immature bitter orange, radish seed, radish seed, and malt sprout; the Chinese medicine composition is composed of the following Chinese medicines in parts by weight: 40-60 parts of Chinaberry bark, 20-40 parts of Stemona, 35-55 parts of Areca, 20-40 parts of Gentiana, 20-40 parts of Andrographis, 10-30 parts of Houttuynia, 20-40 parts of Cyperus, 8-25 parts of Aucklandia, 10-28 parts of Toosendan, 10-28 parts of Citrus aurantium, 5-25 parts of Radish, 20-40 parts of Radish Seed, and 5-25 parts of Malus.

2. The Chinese medicine composition according to claim 1, characterized in that: The Chinese medicine composition is composed of the following Chinese medicines in parts by weight: 45-55 parts of Chinaberry bark, 25-35 parts of Stemona, 40-50 parts of Areca, 25-35 parts of Gentiana, 25-35 parts of Andrographis, 15-25 parts of Houttuynia, 25-35 parts of Cyperus, 10-20 parts of Aucklandia, 12-24 parts of Toosendan fruit, 12-24 parts of Citrus aurantium, 10-20 parts of Radish, 25-35 parts of Radish seed, and 10-20 parts of malt sprout.

3. The Chinese medicine composition according to claim 1, characterized in that The Chinese medicine composition is composed of the following Chinese medicines in parts by weight: 50 parts of Chinese neem bark, 30 parts of stemona, 45 parts of areca nut, 30 parts of gentian, 30 parts of andrographis paniculata, 20 parts of houttuynia, 20 parts of osmanthus, 15 parts of costusroot, 18 parts of toosendan fruit, 18 parts of immature bitter orange, 15 parts of radish seeds, 20 parts of radish seeds, and 15 parts of malt sprout.

4. The Chinese medicine composition according to any one of claims 1 to 3, characterized in that: The preparation method of the Chinese medicine composition is as follows: (1) Crush the material through a 40-60 mesh sieve; (2) Add 10-15 times the amount of water, reflux and extract at 100°C for 2-4 times, each time for 1-3 hours, filter, and combine the filtrates; (3) lowering the temperature of the filtrate to 37-42° C., adding cellulase and ligninase, adjusting the pH to 5.0, and performing ultrasound-assisted enzymatic extraction at an ultrasonic frequency of 30-40 kHz for 55-65 min to obtain an enzyme extract; and boiling for 8 min to inactivate the enzyme; (4) The enzyme extract is concentrated under vacuum to an extract density of 1.05-1.15, spray-dried, and pulverized to prepare a conventional dosage form.

5. The Chinese medicine composition according to claim 4, characterized in that: The mass ratio of the cellulase to the ligninase is 2-3:

1.

6. Use of the Chinese medicine composition according to any one of claims 1 to 3 in the preparation of drugs for preventing and treating chicken coccidiosis.

Images