Myocardial injury sensor based on electromagnetically induced transparency and preparation method thereof

Abstract

The embodiment of the invention relates to the technical field of optical fiber sensors, in particular to a cardiac muscle injury sensor based on electromagnetically induced transparency and a preparation method of the cardiac muscle injury sensor based on electromagnetically induced transparency, the cardiac muscle injury sensor comprises a single-mode optical fiber, a hollow-core optical fiber, a reference microsphere and a sensing microsphere, and one end of the single-mode optical fiber is connected with one end of the hollow-core optical fiber; the reference microsphere and the sensing microsphere are embedded in the hollow optical fiber, and the free end of the hollow optical fiber is processed to form a side opening window; incident light entering from the single-mode optical fiber is coupled into the hollow-core optical fiber at the connecting position, an evanescent field is generated on the surface of the hollow-core optical fiber, then the incident light is sequentially coupled to the reference microsphere and the sensing microsphere which are embedded in the hollow-core optical fiber, and the incident light is propagated on the circumferential annular surfaces of the reference microsphere and the sensing microsphere and excites an optical echo wall mode. And part of light on the reference microsphere and the sensing microsphere is recoupled back to the hollow-core optical fiber and is collected by the single-mode optical fiber. According to the myocardial injury sensor, higher quality factors are realized, and the detection precision is improved.

Description

Technical Field

[0001] The embodiments of the present application relate to the technical field of optical fiber sensors, and in particular to a myocardial injury sensor based on electromagnetic induced transparency and a preparation method thereof. Background Art

[0002] Acute myocardial infarction (AMI) is a serious heart disease, usually caused by obstruction of coronary blood flow, leading to myocardial ischemia and hypoxia, and eventually myocardial necrosis. Cardiac troponin I (cTnI) is a protein in myocardial cells. When myocardial cells are damaged, myocardial troponin I will be released into the blood, so cTnI is an important marker of myocardial damage. In the diagnosis of AMI, the detection of cTnI is very important. By testing the concentration level of cTnI in the blood, doctors can assess the degree of myocardial damage and diagnose AMI. The increase of cTnI usually occurs within a few hours after myocardial infarction. Rapid and accurate cTnI detection can help improve patient survival rate. For the in vitro detection of cTnI, enzyme-linked immunosorbent assay, chemiluminescence method and colloidal gold method are widely used in clinical practice, but these methods have problems such as long detection time, high cost and low sensitivity.

[0003] As an optical sensor, wavelength demodulation fiber optic sensor is widely used in the detection of biochemical markers. By tracking and measuring the characteristic peak (valley) wavelength in the optical fiber sensor spectrum, the optical fiber sensor can realize the quantitative detection of the concentration of the target marker. Therefore, the quality of the characteristic spectrum is directly related to the detection accuracy of the target marker. However, the commonly used optical fiber sensors are limited by the traditional interference waveguide structure, and the spectral quality cannot meet the needs of high-precision biochemical detection. By utilizing the electromagnetically induced transparency effect, the optical fiber sensor can obtain a narrower high quality factor (Q value) characteristic peak (valley), which is beneficial to improve the detection accuracy. However, there is no research on electromagnetically induced transparent optical fiber sensors. It is very important and urgent to develop a myocardial injury sensor based on electromagnetically induced transparency and its preparation method. Summary of the invention

[0004] In order to solve the above technical problems, the embodiments of the present application propose a myocardial injury sensor based on electromagnetic induced transparency and a preparation method, which fully combines the electromagnetic induced transparency effect with the optical fiber sensor, effectively compresses the characteristic spectrum of the whispering gallery mode sensing microcavity, achieves a higher quality factor, and improves the detection accuracy of cTnI, providing a solution with application prospects for the early diagnosis and precision medicine of AMI.

[0005] In a first aspect, an embodiment of the present application proposes a myocardial injury sensor based on electromagnetically induced transparency, comprising: a single-mode optical fiber (11), a hollow-core optical fiber (12), a reference microsphere (13) and a sensing microsphere (14), wherein one end of the single-mode optical fiber (11) is connected to one end of the hollow-core optical fiber (12), the reference microsphere (13) and the sensing microsphere (14) are respectively embedded in the interior of the hollow-core optical fiber (12), the reference microsphere (13) is closer to the connection (16) between the single-mode optical fiber (11) and the hollow-core optical fiber (12) than the sensing microsphere (14), and the free end of the hollow-core optical fiber (12) is processed to have A side open window (15); incident light entering from the single-mode optical fiber (11) is coupled into the hollow-core optical fiber (12) at the connection point (16), and an evanescent field is generated on the surface of the hollow-core optical fiber (12), and then coupled to the reference microsphere (13) and the sensing microsphere (14) embedded in the hollow-core optical fiber (12) in turn, propagates on the circumferential annular surface of the reference microsphere (13) and the sensing microsphere (14) and excites an optical whispering gallery mode, and part of the light on the reference microsphere (13) and the sensing microsphere (14) is recoupled back to the hollow-core optical fiber (12) and collected by the single-mode optical fiber (11).

[0006] Optionally, the sensing microsphere (14) embedded in the hollow-core optical fiber (12) is used as a key sensitive element to capture and bind the cTnI antigen. When the myocardial injury sensor is immersed in a liquid sample to be tested, the liquid sample to be tested contacts the sensing microsphere (14) embedded in the hollow-core optical fiber (12) through a side open window (15) on the hollow-core optical fiber (12). An increase in the cTnI concentration in the liquid sample to be tested will change the effective refractive index of the sensing microsphere (14), thereby causing the reflection spectrum of the myocardial injury sensor to change, causing the narrow characteristic valley wavelength generated by the electromagnetically induced transparency effect to drift. By measuring the drift amount of the characteristic valley wavelength, the cTnI concentration of the liquid sample to be tested can be detected.

[0007] Optionally, the reference microsphere (13) and the sensing microsphere (14) are made of silicon dioxide.

[0008] Optionally, one end of the single-mode optical fiber (11) is connected to one end of the hollow-core optical fiber (12) by fusion splicing, and the connection point (16) between the single-mode optical fiber (11) and the hollow-core optical fiber (12) is a fusion collapse point formed by fusion splicing.

[0009] In the above way, the myocardial injury sensor based on electromagnetically induced transparency adopts a compact size design and economical manufacturing cost, which is particularly suitable for narrow sensing areas with limited space. Its reflective probe structure is not only easy to operate, but also has potential implantable in vivo detection capabilities. By selecting single-mode optical fiber, hollow-core optical fiber and silica microspheres as basic materials, the myocardial injury sensor achieves a significant reduction in cost while ensuring detection performance. In addition, the standardized design and mass production capabilities of the myocardial injury sensor, combined with the replaceability of antibodies on the surface of the sensing microspheres, provide flexibility and convenience to meet the diverse needs of biochemical marker detection. The myocardial injury sensor utilizes the electromagnetically induced transparency effect to further compress the characteristic spectrum of the whispering gallery mode sensing microcavity, thereby achieving a higher quality factor and improving the detection accuracy of cTnI, providing a promising solution for the early diagnosis and precision medicine of AMI.

[0010] In a second aspect, an embodiment of the present application proposes a method for preparing a myocardial injury sensor based on electromagnetically induced transparency, the method comprising: S21, using a fiber fusion splicer to fuse a section of single-mode optical fiber with a flat end face with a section of hollow-core optical fiber; S22, fixing the fused single-mode optical fiber and the hollow-core optical fiber on a fiber cleaver with a microscopic observation function, cutting off the excess hollow-core optical fiber, and obtaining a single-mode-hollow-core optical fiber structure; S23, using a femtosecond laser to process the unfused end of the hollow-core optical fiber in the single-mode-hollow-core optical fiber structure to form a side open window, and obtaining a single-mode-hollow-core optical fiber structure with a side open window; S24, selecting a number of silica microspheres and immersing them in an acetone solution, performing ultrasonic cleaning and then cleaning with ethanol, and drying the cleaned silica microspheres for standby use; S25, selecting a portion of the standby silica microspheres The microspheres are immersed in a poly-lysine solution so that a poly-lysine layer is formed on the surface of a portion of the selected spare silica microspheres; S26, the silica microspheres with the poly-lysine layer on the surface are washed with deionized water or phosphate buffer, and then immersed in an N-hydroxysuccinimide solution to activate the amino groups on the poly-lysine layer; S27, the activated silica microspheres are immersed in a phosphate buffer solution of cTnI antibody to complete the anchoring of the cTnI antibody on the poly-lysine layer; S28, the anchored silica microspheres are immersed in a phosphate buffer solution of bovine serum albumin to reduce nonspecific adsorption, and finally a sensing microsphere is obtained; S29, the unselected silica microspheres are embedded as reference microspheres in a single-mode-hollow-core optical fiber structure with a side open window, and then the sensing microspheres are embedded to obtain a myocardial injury sensor.

[0011] Optionally, in S25, a portion of the reserved silica microspheres is selected and immersed in the poly-lysine solution for 20 to 40 minutes.

[0012] Optionally, in S26, the silica microspheres having a poly-lysine layer on the surface are washed with deionized water or phosphate buffer and then immersed in an N-hydroxysuccinimide solution for 20 to 40 minutes.

[0013] Optionally, in S27, the activated silica microspheres are immersed in a phosphate buffered saline solution containing cTnI antibodies for 20 to 40 minutes.

[0014] Optionally, the anchored silica microspheres are immersed in a phosphate buffer solution of bovine serum albumin for 20 to 40 minutes.

[0015] In a third aspect, an embodiment of the present application proposes a computer-readable storage medium storing a computer program, which, when executed by a processor, can implement a method for preparing a myocardial injury sensor based on electromagnetically induced transparency as described in the first aspect above.

[0016] It can be understood that the beneficial effects of the second to third aspects mentioned above can be found in the relevant description of the first aspect mentioned above, and will not be repeated here. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the related technologies, the drawings required for use in the embodiments of the present application or the related technical descriptions will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0018] Figure 1 is a schematic diagram of a specific structure of a myocardial injury sensor based on electromagnetic induced transparency provided in one embodiment of the present application;

[0019] Figure 2 It is a front view of a specific structure of a myocardial injury sensor based on electromagnetic induction transparency provided in one embodiment of the present application;

[0020] Figure 3 is a top view of a specific structure of a myocardial injury sensor based on electromagnetic induced transparency provided in one embodiment of the present application;

[0021] Figure 4 It is a flow chart of a method for preparing an electromagnetically induced transparent myocardial injury sensor provided in another embodiment of the present application. DETAILED DESCRIPTION

[0022] In order to make the purpose, technical scheme and advantages of the embodiments of the present application clearer, the embodiments of the present application will be described in detail below in conjunction with the accompanying drawings. In the various embodiments of the present application, many technical details are proposed in order to make the reader better understand the present application. However, even without these technical details and various changes and modifications based on the following embodiments, the technical scheme claimed in the present application can also be implemented. The division of the following embodiments is only for the convenience of description, and the specific implementation mode of the present application should not constitute any limitation. The various embodiments can be combined with each other and referenced to each other under the premise of no contradiction.

[0023] An embodiment of the present application proposes a myocardial injury sensor based on electromagnetic induced transparency. The implementation details of the myocardial injury sensor based on electromagnetic induced transparency proposed in this embodiment are specifically described below. The following content is only the implementation details provided for the convenience of understanding and is not necessary for the implementation of this solution.

[0024] The specific structure of the electromagnetically induced transparent myocardial injury sensor proposed in this embodiment is as follows: Figure 1 As shown, its main view is as follows Figure 2 As shown, its top view is as Figure 3 As shown, the myocardial injury sensor includes: a single-mode optical fiber 11, a hollow-core optical fiber 12, a reference microsphere 13 and a sensing microsphere 14.

[0025] One end of the single-mode optical fiber 11 is connected to one end of the hollow-core optical fiber 12, and the reference microsphere 13 and the sensing microsphere 14 are respectively embedded in the interior of the hollow-core optical fiber 12. The reference microsphere 13 is closer to the connection 16 between the single-mode optical fiber 11 and the hollow-core optical fiber 12 than the sensing microsphere 14. The free end of the hollow-core optical fiber 12 is processed to form a side open window 15.

[0026] The incident light entering from the single-mode optical fiber 11 will be coupled into the hollow-core optical fiber 12 at the connection 16, and an evanescent field will be generated on the surface of the hollow-core optical fiber 12, and then coupled to the reference microsphere 13 and the sensing microsphere 14 embedded in the hollow-core optical fiber 12 in turn, propagating on the circumferential annular surface of the reference microsphere 13 and the sensing microsphere 14 and exciting the optical whispering gallery mode. Part of the light on the reference microsphere 13 and the sensing microsphere 14 will be recoupled back to the hollow-core optical fiber 12 and collected by the single-mode optical fiber 11. During the propagation of the whispering gallery mode in the reference microsphere 13 and the sensing microsphere 14, the reflection and loss of light lead to coupling between the clockwise and counterclockwise modes, breaking the degeneracy and causing mode splitting, which is manifested as a narrower transparent valley on the characteristic peak in the reflection spectrum, that is, the electromagnetically induced transparency phenomenon in the coupled resonant cavity. Therefore, the sensor waveguide structure can be equivalent to a typical parallel microring resonator and produce an electromagnetically induced transparency effect.

[0027] The sensing microsphere 14 embedded in the hollow-core optical fiber 12 is used as a key sensitive element to capture and bind the cTnI antigen. When the myocardial injury sensor is immersed in the liquid sample to be tested, the liquid sample to be tested contacts the sensing microsphere 14 embedded in the hollow-core optical fiber 12 through the side open window 15 on the hollow-core optical fiber 12. The increase in the cTnI concentration in the liquid sample to be tested will change the effective refractive index of the sensing microsphere 14, thereby changing the reflection spectrum of the myocardial injury sensor, and the narrow characteristic valley wavelength generated by the electromagnetic induced transparency effect will drift. By measuring the drift of the characteristic valley wavelength, the cTnI concentration of the liquid sample to be tested is detected. And the detection accuracy is very high.

[0028] In one example, the reference microsphere 13 and the sensing microsphere 14 are made of silicon dioxide.

[0029] In one example, one end of the single-mode optical fiber 11 is connected to one end of the hollow-core optical fiber 12 by fusion splicing, and the connection 16 between the single-mode optical fiber 11 and the hollow-core optical fiber 12 is a fusion collapse formed by fusion splicing.

[0030] In this embodiment, the myocardial injury sensor based on electromagnetically induced transparency adopts a compact size design and economical manufacturing cost, which is particularly suitable for narrow sensing areas with limited space. Its reflective probe structure is not only easy to operate, but also has potential implantable in vivo detection capabilities. By selecting single-mode optical fiber, hollow-core optical fiber and silica microspheres as basic materials, the myocardial injury sensor achieves a significant reduction in cost while ensuring detection performance. In addition, the standardized design and mass production capabilities of the myocardial injury sensor, combined with the replaceability of antibodies on the surface of the sensing microspheres, provide flexibility and convenience to meet the diverse needs of biochemical marker detection. The myocardial injury sensor utilizes the electromagnetically induced transparency effect to further compress the characteristic spectrum of the whispering gallery mode sensing microcavity, thereby achieving a higher quality factor and improving the detection accuracy of cTnI, providing a solution with application prospects for the early diagnosis and precision medicine of AMI.

[0031] It is worth mentioning that all modules involved in this embodiment are logic modules. In practical applications, a logic unit can be a physical unit, a part of a physical unit, or a combination of multiple physical units. In addition, in order to highlight the innovative part of this application, this embodiment does not introduce units that are not closely related to solving the technical problems proposed by this application, but this does not mean that there are no other units in this embodiment.

[0032] Another embodiment of the present application proposes a method for preparing a myocardial injury sensor based on electromagnetic induced transparency. The implementation details of the method for preparing a myocardial injury sensor based on electromagnetic induced transparency proposed in this embodiment are described in detail below. The following content is only the implementation details provided for the convenience of understanding and is not necessary for the implementation of this solution.

[0033] The specific process of the preparation method of the electromagnetically induced transparent myocardial injury sensor proposed in this embodiment can be as follows: Figure 4 As shown, including:

[0034] S21, use a fiber fusion splicer to fuse a section of single-mode optical fiber with a flat end face with a section of hollow-core optical fiber.

[0035] In the specific implementation, when preparing the myocardial injury sensor, first prepare the main body of the myocardial injury sensor, select a section of single-mode optical fiber with a smooth end face and a section of hollow-core optical fiber with a smooth end face, and use a fiber fusion splicer to fuse one end of the single-mode optical fiber with one end of the hollow-core optical fiber.

[0036] S22, fixing the fused single-mode optical fiber and hollow-core optical fiber on an optical fiber cutter with a microscopic observation function, cutting off excess hollow-core optical fiber, and obtaining a single-mode-hollow-core optical fiber structure.

[0037] In a specific implementation, after one end of a single-mode optical fiber is fused with one end of a hollow-core optical fiber, the fused single-mode optical fiber and the hollow-core optical fiber are fixed on a fiber cleaver with a microscopic observation function, and the excess hollow-core optical fiber is cut off to obtain a single-mode-hollow-core optical fiber structure.

[0038] S23, processing the unfused end of the hollow-core optical fiber in the single-mode-hollow-core optical fiber structure to form a side open window by using a femtosecond laser processing method, so as to obtain a single-mode-hollow-core optical fiber structure with a side open window.

[0039] In the specific implementation, the obtained single-mode-hollow-core fiber structure is the main body of the myocardial injury sensor. Next, the details of the myocardial injury sensor are prepared. The unfused end (free end) of the hollow-core fiber in the single-mode-hollow-core fiber structure is processed into a side open window using a femtosecond laser processing method to obtain a single-mode-hollow-core fiber structure with a side open window. At this point, most of the myocardial injury sensor has been prepared, and only the reference microspheres and sensing microspheres have not yet been prepared and embedded.

[0040] S24, select a number of silica microspheres and immerse them in an acetone solution, perform ultrasonic cleaning and then use ethanol to clean them, and dry the cleaned silica microspheres for later use.

[0041] In a specific implementation, after preparing reference microspheres and sensor microspheres, several silica microspheres are selected and immersed in an acetone solution. After ultrasonic cleaning for 30 minutes, they are cleaned with ethanol. The cleaned silica microspheres are dried and set aside. These spare silica microspheres are reference microspheres.

[0042] S25, selecting a portion of the spare silica microspheres and immersing them in a poly-lysine solution, so that a poly-lysine layer is formed on the surface of the selected portion of the spare silica microspheres.

[0043] In a specific implementation, a portion of the spare silica microspheres is selected and immersed in a poly-lysine solution for 20 to 40 minutes, so that a poly-lysine layer is formed on the surface of the selected portion of the spare silica microspheres.

[0044] S26, washing the silica microspheres having a poly-lysine layer on the surface with deionized water or phosphate buffer, and then immersing them in an N-hydroxysuccinimide solution to activate the amino groups on the poly-lysine layer.

[0045] In a specific implementation, the polylysine layer has been formed, and the silica microspheres with the polylysine layer on the surface are washed with deionized water or phosphate buffer, and then immersed in an N-hydroxysuccinimide solution for 20 to 40 minutes to activate the amino groups on the polylysine layer.

[0046] S27, immersing the activated silica microspheres in a phosphate buffer solution of cTnI antibody to anchor the cTnI antibody on the poly-lysine layer.

[0047] In a specific implementation, after activation is completed, the activated silica microspheres are immersed in a phosphate buffer solution of cTnI antibody for 20 to 40 minutes to complete the anchoring of the cTnI antibody on the poly-lysine layer.

[0048] S28, immersing the anchored silica microspheres in a phosphate buffer solution of bovine serum albumin to reduce nonspecific adsorption, and finally obtaining sensing microspheres.

[0049] In a specific implementation, after the anchoring is completed, the anchored silica microspheres are immersed in a phosphate buffer solution of bovine serum albumin for 20 to 40 minutes to reduce nonspecific adsorption, and finally the sensing microspheres are obtained.

[0050] S29, the unselected silica microspheres are embedded as reference microspheres into a single-mode hollow-core optical fiber structure with a side open window, and then the sensing microspheres are embedded to obtain a myocardial injury sensor.

[0051] In the specific implementation, the reference microspheres and the sensing microspheres have been prepared, and the unselected silica microspheres are embedded as reference microspheres into a single-mode hollow-core optical fiber structure with a side open window, and then the sensing microspheres are embedded to obtain a myocardial injury sensor.

[0052] The step division of the above methods is only for clear description. When implemented, they can be combined into one step or some steps can be split and decomposed into multiple steps. As long as they include the same logical relationship, they are all within the scope of protection of this application; adding insignificant modifications to the algorithm or process or introducing insignificant designs without changing the core design of the algorithm and process are all within the scope of protection of this application.

[0053] It is not difficult to find that this embodiment is a preparation method embodiment corresponding to the above-mentioned myocardial injury sensor embodiment, and this embodiment can be implemented in conjunction with the above-mentioned myocardial injury sensor embodiment. The relevant technical details and technical effects mentioned in the above-mentioned embodiments are still valid in this embodiment, and in order to reduce repetition, they are not repeated here. Accordingly, the relevant technical details mentioned in this embodiment can also be applied in the above-mentioned embodiments.

[0054] Another embodiment of the present application provides a computer-readable storage medium storing a computer program. When the computer program is executed by a processor, it can implement a method for preparing a myocardial injury sensor based on electromagnetically induced transparency as described in the above-mentioned preparation method embodiments.

[0055] That is, those skilled in the art can understand that all or part of the steps in the above-mentioned embodiment method can be completed by instructing the relevant hardware through a program, and the program is stored in a storage medium, including a number of instructions to enable a device (which can be a single-chip microcomputer, chip, etc.) or a processor to execute all or part of the steps of the method described in each embodiment of the present application. The aforementioned storage medium includes: U disk, mobile hard disk, ROM (Read-Only Memory), RAM (Random Access Memory), disk or optical disk and other media that can store program codes.

[0056] Those skilled in the art will appreciate that the above embodiments are specific embodiments for implementing the present application, and in actual applications, various changes may be made thereto in form and detail without departing from the spirit and scope of the present application.

Claims

1. A myocardial injury sensor based on electromagnetically induced transparency, characterized in that: include: A single-mode optical fiber (11), a hollow-core optical fiber (12), a reference microsphere (13) and a sensing microsphere (14), wherein one end of the single-mode optical fiber (11) is connected to one end of the hollow-core optical fiber (12), the reference microsphere (13) and the sensing microsphere (14) are respectively embedded in the hollow-core optical fiber (12), the reference microsphere (13) is closer to the connection (16) between the single-mode optical fiber (11) and the hollow-core optical fiber (12) than the sensing microsphere (14), and the free end of the hollow-core optical fiber (12) is processed to form a side open window (15); Incident light entering from a single-mode optical fiber (11) is coupled into a hollow-core optical fiber (12) at a connection point (16), and generates an evanescent field on the surface of the hollow-core optical fiber (12), and then sequentially couples to a reference microsphere (13) and a sensing microsphere (14) embedded in the hollow-core optical fiber (12), propagates on the circumferential annular surfaces of the reference microsphere (13) and the sensing microsphere (14), and excites an optical whispering gallery mode, and part of the light on the reference microsphere (13) and the sensing microsphere (14) is recoupled back into the hollow-core optical fiber (12) and collected by the single-mode optical fiber (11).

2. The electromagnetically induced transparent myocardial injury sensor according to claim 1, characterized in that: The sensing microsphere (14) embedded in the hollow-core optical fiber (12) is used as a key sensitive element to capture and bind the cTnI antigen. When the myocardial injury sensor is immersed in a liquid sample to be tested, the liquid sample to be tested contacts the sensing microsphere (14) embedded in the hollow-core optical fiber (12) through a side open window (15) on the hollow-core optical fiber (12). An increase in the cTnI concentration in the liquid sample to be tested will change the effective refractive index of the sensing microsphere (14), thereby causing the reflection spectrum of the myocardial injury sensor to change, and the narrow characteristic valley wavelength generated by the electromagnetically induced transparency effect to drift. By measuring the drift amount of the characteristic valley wavelength, the cTnI concentration of the liquid sample to be tested can be detected.

3. The electromagnetically induced transparent myocardial injury sensor according to claim 2, characterized in that: The reference microsphere (13) and the sensing microsphere (14) are made of silicon dioxide.

4. The electromagnetically induced transparent myocardial injury sensor according to claim 2, characterized in that: One end of the single-mode optical fiber (11) and one end of the hollow-core optical fiber (12) are connected by fusion splicing, and the connection point (16) between the single-mode optical fiber (11) and the hollow-core optical fiber (12) is a fusion collapse point formed by fusion splicing.

5. A method for preparing a myocardial injury sensor based on electromagnetic induction transparency, characterized in that: include: S21, using a fiber fusion splicer to fusion-splice a section of single-mode optical fiber with a flat end surface with a section of hollow-core optical fiber; S22, fixing the fused single-mode optical fiber and hollow-core optical fiber on an optical fiber cleaver with a microscopic observation function, cutting off excess hollow-core optical fiber, and obtaining a single-mode-hollow-core optical fiber structure; S23, processing the unfused end of the hollow-core optical fiber in the single-mode-hollow-core optical fiber structure to form a side open window by using a femtosecond laser processing method, so as to obtain a single-mode-hollow-core optical fiber structure with a side open window; S24, selecting a plurality of silica microspheres and immersing them in an acetone solution, performing ultrasonic cleaning and then cleaning them with ethanol, and drying the cleaned silica microspheres for later use; S25, selecting a portion of the silica microspheres for standby use and immersing them in a poly-lysine solution, so that a poly-lysine layer is formed on the surface of the selected portion of the silica microspheres for standby use; S26, washing the silica microspheres with a poly-lysine layer on the surface with deionized water or phosphate buffer, and then immersing them in an N-hydroxysuccinimide solution to activate the amino groups on the poly-lysine layer; S27, immersing the activated silica microspheres in a phosphate buffer solution of cTnI antibody to anchor the cTnI antibody on the poly-lysine layer; S28, immersing the anchored silica microspheres in a phosphate buffer solution of bovine serum albumin to reduce nonspecific adsorption, and finally obtaining sensing microspheres; S29, the unselected silica microspheres are embedded as reference microspheres into a single-mode hollow-core optical fiber structure with a side open window, and then the sensing microspheres are embedded to obtain a myocardial injury sensor.

6. The method for preparing a myocardial injury sensor based on electromagnetic induction transparency according to claim 5, characterized in that: In S25, a portion of the reserved silica microspheres is selected and immersed in the poly-lysine solution for 20 to 40 minutes.

7. The method for preparing a myocardial injury sensor based on electromagnetically induced transparency according to claim 6, characterized in that: In S26, the silica microspheres having a poly-lysine layer on the surface are washed with deionized water or phosphate buffer and then immersed in an N-hydroxysuccinimide solution for 20 to 40 minutes.

8. The method for preparing a myocardial injury sensor based on electromagnetically induced transparency according to claim 7, characterized in that: In S27, the activated silica microspheres are immersed in a phosphate buffered saline solution containing cTnI antibodies for 20 to 40 minutes.

9. The method for preparing the electromagnetically induced transparent myocardial injury sensor according to claim 8, characterized in that: The anchored silica microspheres are immersed in a phosphate buffer solution of bovine serum albumin for 20 to 40 minutes.

10. A computer-readable storage medium storing a computer program, characterized in that: When the computer program is executed by a processor, it can implement a method for preparing a myocardial injury sensor based on electromagnetic induced transparency as described in any one of claims 5 to 9.

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