A method for effectively inhibiting the propagation of fruit fly larvae in the nutrient soil when planting arabidopsis

Abstract

This invention discloses a method for effectively inhibiting the reproduction of fruit fly larvae in the nutrient soil during Arabidopsis thaliana cultivation, relating to the field of soil science. The method includes: ① planting Arabidopsis thaliana seedlings; ② preparation of a non-absorbent material; ③ application of the non-absorbent material; ④ watering in a tray. This invention has the following advantages and positive effects: By preparing a non-absorbent material, this method maintains the continuous dryness of the surface nutrient substrate, effectively inhibiting the reproduction of fruit flies on the surface of the nutrient soil during Arabidopsis thaliana cultivation. This prevents Arabidopsis thaliana seedlings from being eaten by fruit fly larvae, or even the entire plant from being eaten and dying. It also effectively inhibits the outbreak of surface terrestrial fungi, helping researchers using Arabidopsis thaliana as experimental material to efficiently and labor-savingly cultivate plant materials suitable for measuring experimental data in greenhouses.

Description

Technical Field

[0001] This invention relates to the field of soil science, specifically to a method for effectively inhibiting the reproduction of fruit fly larvae in the nutrient soil during Arabidopsis thaliana cultivation. Background Technology

[0002] Arabidopsis thaliana is a model plant for genetic research. When grown in greenhouses, Arabidopsis thaliana is susceptible to various pests and diseases, with fruit flies being the most prevalent. Effectively controlling the feeding of Arabidopsis thaliana by fruit fly larvae is a crucial problem that researchers using Arabidopsis thaliana as experimental material urgently need to solve. Adult fruit flies are small flying insects that lay eggs on the surface of the Arabidopsis thaliana substrate. After hatching, the eggs produce transparent white larvae that primarily feed on the leaves of Arabidopsis thaliana close to the surface substrate. The moist environment of the substrate surface facilitates the hatching of fruit fly eggs. If left uncontrolled, fruit fly larvae multiply rapidly on the substrate surface, potentially devouring entire young Arabidopsis thaliana seedlings. Currently, methods for controlling fruit fly reproduction in the surface substrate of Arabidopsis thaliana seedlings mainly include using fly traps to capture adult flies, adding pesticides to the substrate, sprinkling vermiculite on the substrate surface to control surface soil moisture, removing surface soil containing fruit fly larvae, reducing watering, and spraying pesticides. However, while these methods are effective, they are insufficient to suppress the reproduction of fruit flies in Arabidopsis thaliana nutrient soil, thus affecting the accurate collection of experimental data.

[0003] To date, there are no reported methods in the existing technology for effectively suppressing the reproduction of Arabidopsis thaliana fruit flies without spraying pesticides. Summary of the Invention

[0004] The purpose of this invention is to overcome the problems and shortcomings of the existing technology and provide a simple and effective method to inhibit the reproduction of fruit fly larvae in the nutrient soil during Arabidopsis planting. Specifically, it inhibits the reproduction of fruit fly larvae on the surface of the nutrient soil, avoids Arabidopsis seedlings from being eaten by fruit fly larvae or even completely devoured by them, and helps scientific research on Arabidopsis as the research object.

[0005] The objective of this invention is achieved as follows:

[0006] This method includes the following steps:

[0007] ① Planting of Arabidopsis thaliana seedlings

[0008] Transplant Arabidopsis seedlings with 2 leaves into fresh substrate free of fruit fly eggs and grow them in the Arabidopsis chamber for 7-10 days.

[0009] ② Preparation of non-absorbent materials

[0010] Pindstrup, a Danish-made moss peat moss, is one of the commonly used nutrient substrates for cultivating Arabidopsis thaliana in the laboratory. Take an appropriate amount of this nutrient substrate, crush it into small pieces by hand, and place it in an oven at 85°C to remove the moisture from the substrate. After the substrate becomes a non-absorbent material, fill the nutrient pot with this substrate, place it in a tray, and pour water into the tray. The substrate in the nutrient pot cannot naturally absorb water from the bottom up.

[0011] ③ Use of non-absorbent materials

[0012] Arabidopsis thaliana plants grown in the Arabidopsis thaliana chamber for 7-10 days are established and have 4-6 leaves. The surface of the nutrient soil in which the Arabidopsis thaliana is growing is covered with a prepared non-absorbent material, with a thickness of about 0.5-1 cm. The tray is gently shaken or the leaves of the Arabidopsis thaliana covered with the non-absorbent material are released by hand.

[0013] ④ Watering in the tray

[0014] Place the small pots of Arabidopsis thaliana in a tray; later, use the method of watering the tray and letting the nutrient soil absorb the water naturally to ensure that the non-absorbent material on the surface remains dry, thereby maintaining a dry environment on the surface of the nutrient soil for Arabidopsis thaliana growth.

[0015] Working principle:

[0016] After 7-10 days of transplanting into the nutrient soil, the seedlings have established themselves, with 4-6 leaves and a certain height. A 0.5-1cm layer of non-absorbent material can be placed under the leaves without affecting the normal growth of the Arabidopsis seedlings. By laying a layer of non-absorbent material on the surface of the Arabidopsis nutrient soil, the small number of fruit fly eggs that may have been laid before the material was laid will die due to the dry environment during larval hatching. Furthermore, eggs laid on the non-absorbent material during subsequent growth will not hatch due to the lack of moisture. This effectively inhibits the hatching of fruit flies on the surface of the nutrient soil during Arabidopsis planting and controls fruit fly reproduction in the Arabidopsis chamber.

[0017] The purpose of this method:

[0018] This method can be used to prevent and suppress the reproduction of fruit flies in Arabidopsis thaliana chambers.

[0019] This invention has the following advantages and positive effects:

[0020] This method, by preparing a non-absorbent material to maintain the continuous dryness of the surface nutrient substrate, can effectively inhibit the reproduction of fruit flies on the surface of the nutrient soil during Arabidopsis planting. This prevents Arabidopsis seedlings from being eaten by fruit fly larvae or even dying from being completely consumed by them. It can also effectively inhibit the outbreak of surface terreus.

[0021] This will help researchers using Arabidopsis thaliana as experimental material to efficiently and labor-savingly cultivate plant materials in greenhouses that can be used to measure experimental data. Attached Figure Description

[0022] Figure 1 This is a flowchart illustrating the process of inhibiting fruit fly reproduction in the potting soil during Arabidopsis thaliana cultivation. (See the flowchart.)

[0023] 1—Transplant seedlings into fresh substrate;

[0024] 2—Preparation of non-absorbent materials;

[0025] 3. Cover the seedlings with non-absorbent material 7-10 days after transplanting;

[0026] 4—Water is injected into the tray and absorbed naturally.

[0027] Figure 2 The figure shows the growth status of Arabidopsis thaliana in the topsoil with and without a non-absorbent material covering it.

[0028] a— Arabidopsis thaliana was grown in a room infested with fruit flies. The top two pots were covered with a non-absorbent material, while the bottom two pots were not covered with a non-absorbent material.

[0029] b—This is a magnified view of the uncovered, non-absorbent material surface, where many white, transparent fruit fly larvae can be seen. The light green residue consists of stem segments left behind after the fruit fly larvae have eaten the Arabidopsis seedlings. Detailed Implementation

[0030] The following is a detailed description with reference to the accompanying drawings and embodiments:

[0031] ① Planting of Arabidopsis thaliana seedlings

[0032] Arabidopsis seedlings grown on MS plates for about 4 days were transplanted into fresh substrate free of fruit fly eggs, watered thoroughly, and grown in the Arabidopsis chamber for 7-10 days. Figure 1 ).

[0033] ② Preparation of non-absorbent materials

[0034] Take an appropriate amount of Danish Pinss imported moss peat moss, crush it into small granules by hand, and dry it in an oven at 85℃ for at least 3 hours to remove moisture from the substrate. Once the substrate is completely dry and has cooled to room temperature, store it in a clean, sealed plastic bag for later use. Figure 1 ).

[0035] ③ Use of non-absorbent materials

[0036] Arabidopsis thaliana plants grown in the Arabidopsis thaliana chamber for 7-10 days, at which point they have established themselves and have 4-6 leaves; a prepared non-absorbent material is then spread over the entire surface of the nutrient soil in which the Arabidopsis thaliana is growing, to a thickness of about 0.5-1 cm; the tray is then gently shaken or manually removed from the surface by releasing the Arabidopsis thaliana leaves covered with the non-absorbent material. Figure 1 If fruit fly outbreaks occur in Arabidopsis thaliana chambers and are not controlled, such as... Figure 2 The transplanted Arabidopsis seedlings were severely eaten until the entire plant was completely devoured.

[0037] ④ Watering in the tray

[0038] Pour an appropriate amount of water into the tray containing the small pots of Arabidopsis thaliana. The dry substrate on the surface does not actively absorb water, thus maintaining a dry environment on the surface. Figure 1 Later, the watering method of continuously injecting water into the tray and allowing the nutrient soil below the non-absorbent material to absorb the water naturally can keep the non-absorbent material on the surface of Arabidopsis thaliana dry throughout the entire growth period, thereby maintaining a dry environment on the surface of the Arabidopsis thaliana growth substrate and effectively inhibiting the reproduction of fruit flies in the Arabidopsis thaliana chamber.

Claims

1. A method for inhibiting the reproduction of fruit fly larvae in the nutrient soil during Arabidopsis thaliana cultivation, characterized in that... Includes the following steps: ① Planting of Arabidopsis thaliana seedlings Transplant Arabidopsis seedlings with 2 leaves into fresh substrate free of fruit fly eggs and grow them in the Arabidopsis chamber for 7-10 days. ② Preparation of non-absorbent materials Pins' substrate, imported directly from Denmark, is one of the commonly used nutrient substrates for cultivating Arabidopsis thaliana in the laboratory. Take an appropriate amount of this nutrient substrate, crush it into small pieces by hand, and place it in an oven at 85°C to remove the moisture from the substrate. After the substrate becomes a non-absorbent material, fill the nutrient pot with this substrate, place it in a tray, and pour water into the tray. The substrate in the nutrient pot cannot naturally absorb water from the bottom up. ③ Use of non-absorbent materials Arabidopsis thaliana plants grown in the Arabidopsis thaliana chamber for 7-10 days are established and have 4-6 leaves. The surface of the nutrient soil in which the Arabidopsis thaliana is growing is covered with a prepared non-absorbent material, with a thickness of 0.5-1 cm. The tray is gently shaken or the leaves of the Arabidopsis thaliana covered with the non-absorbent material are released manually. ④ Watering in the tray Place the small pots of Arabidopsis thaliana growing in a tray; later, use a watering method of pouring water into the tray and letting the substrate absorb it naturally to ensure that the non-absorbent material on the surface remains dry, thereby maintaining a dry environment on the surface of the Arabidopsis thaliana growing substrate.

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