Temperature control method for single-molecule immunodetection analyzer

Abstract

The invention relates to the technical field of temperature control, and discloses a temperature control method for a single molecule immunodetection analyzer, which comprises the following steps: placing a reaction cup on a heated body, and forming direct contact with the heated body to form heat conduction; the temperature of a heated body is monitored through a heated body temperature sensor; monitoring the temperature of the space where the heated body is located through a space temperature sensor; reading temperature values of the heated body temperature sensor and the space temperature sensor; and controlling the temperature of the heated body and the space temperature through the heater according to the temperature value. According to the invention, environment temperature control is brought into the design of overall temperature control, so that the problem that the temperature control effect of an instrument is greatly influenced by external environment temperature change is solved, the actual incubation reaction effect is further ensured, and meanwhile, under the condition of external special disturbance, the temperature control effect is greatly improved. The system can perform rapid self-tuning to greatly reduce incubation differences caused by temperature fluctuations.

Description

Technical Field

[0001] The invention relates to the technical field of temperature control, and in particular to a temperature control method for a single molecule immunoassay analyzer. Background Art

[0002] Traditional single-molecule immunoassay methods usually require a lot of time and manpower, and manual operations are prone to introduce errors.

[0003] Single-molecule immunoassays require the detection of tiny signal changes, requiring high sensitivity and precision measurements. Automated systems can improve the accuracy and reproducibility of analyses by optimizing processes, reducing operator intervention, and standardizing processing.

[0004] The automated single-molecule immunoassay system can process large batches of samples and perform multiple experiments simultaneously. This enables researchers to scale up their experiments, process more samples, and conduct a wider range of experiments, accelerating the speed and progress of scientific research.

[0005] In the process of research and diagnosis, the complexity of many samples continues to increase. Automated single-molecule immunoassay systems are able to process complex biological samples such as blood, tissues and body fluids, and provide accurate analysis results. Automated single-molecule immunoassay systems can integrate steps such as sample processing, experimental operation, data acquisition and analysis to achieve a highly automated workflow. This helps reduce experimental errors, improve the standardization of experiments, and promote laboratory work efficiency.

[0006] In a Chinese patent with publication number CN 117192094 A, an automated single molecule immunoassay system and method are disclosed, which relate to the technical field of automated single molecule immunoassay. The automated single molecule immunoassay system and method are composed of a fluid processing module, a sensor module, a sample processing module, an analysis module, a control module, a data processing and analysis module, a sample storage and transmission module, a cleaning module, a temperature control module, and an automated sampling module. The liquid pump is a syringe pump, a peristaltic pump, or a pressure-driven pump. The valve is a solenoid valve, a microfluidic valve, or a rotary valve. The mixer is a mechanical stirrer, a microfluidic mixer, or an ultrasonic mixer. The filter is a microporous filter membrane, a filter element, or a paper filter. The temperature control device is a heater, a cooler, or a temperature control module. The liquid pump is used to provide fluid delivery and control. The sensor module includes a probe (or antibody), a recognition element, a sensor chip or a substrate, a signal converter, and a signal detection system.

[0007] In single-molecule immunoassays, incubation usually needs to be carried out under specific temperature conditions to ensure the accuracy and reliability of the results. At the same time, changes in incubation temperature will also affect the speed and specificity of the immune response. The instrument usually needs to have good temperature stability to ensure that temperature changes during the reaction will not interfere with the results, thereby ensuring the consistency of the immune response. When the prior art is used, temperature control is only achieved through a temperature control device (heater, cooler or temperature control module), and a single heated body heater is used for temperature control. The actual temperature control effect will still be affected by changes in ambient temperature. At the same time, the common temperature control system has a long setting time period when it is subject to external interference, making it difficult to form rapid setting. Summary of the invention

[0008] The purpose of the present invention is to solve the problems existing in the prior art and to propose a temperature control method for a single molecule immunoassay analyzer.

[0009] In order to achieve the above object, the present invention adopts the following technical solutions:

[0010] A temperature control method for a single molecule immunoassay analyzer comprises the following steps:

[0011] Place the reaction cup on the heated body to form direct contact with the heated body to achieve heat conduction;

[0012] Monitor the temperature of the heated body by means of a heated body temperature sensor;

[0013] Monitor the temperature of the space where the heated body is located through a space temperature sensor;

[0014] Read the temperature values ​​of the heated body temperature sensor and the space temperature sensor;

[0015] According to the temperature value, the temperature of the heated object and the space temperature are controlled by the heater.

[0016] The present invention incorporates the control of ambient temperature into the design of overall temperature control, thereby solving the problem that the temperature control effect of the instrument is greatly affected by changes in the external ambient temperature, thereby ensuring the effect of the actual incubation reaction. At the same time, in the case of special external disturbances, the system can perform rapid self-tuning to greatly reduce the incubation differences caused by temperature fluctuations.

[0017] Preferably, the reading time interval for reading the temperature values ​​of the heated body temperature sensor and the space temperature sensor is 0.1s to 0.3s, and the reading interval is 0.1s to 0.3s, which can achieve precise temperature control and maintain temperature stability.

[0018] Preferably, when controlling the temperature of the heated body and the space temperature by a heater according to the temperature value, it also includes controlling the temperature of the heated body and the space temperature by a cooler, which provides a cooling function in addition to the heating function.

[0019] A temperature control device for a single molecule immunoassay analyzer is used to implement a temperature control method for a single molecule immunoassay analyzer, comprising an incubation cavity, wherein a heat receiving body is arranged inside the incubation wall, a placement groove is arranged on the top of the heat receiving body, the placement groove is adapted to a reaction cup, a heat receiving body temperature sensor and a heat receiving body heater are arranged inside the heat receiving body, the heat receiving body temperature sensor and the heat receiving body heater form heat conduction with the heat receiving body, and a space heater and a space temperature sensor are also arranged inside the incubation cavity.

[0020] Preferably, the heated body heater and the space heater are both electric heaters.

[0021] Preferably, a fan is provided at the bottom of the space heater, which can blow cool air to reduce the temperature in the incubation chamber when the space heater is not started, and can blow hot air to promote uniform temperature in the incubation chamber when the space heater is started.

[0022] A temperature control system for a single molecule immunoassay analyzer is used in a temperature control device for a single molecule immunoassay analyzer, comprising a control processor and a heater drive module, wherein the heated body temperature sensor and the space temperature sensor are both electrically connected to the control processor, and the heater drive module is electrically connected to the heated body heater, the space heater and the control processor.

[0023] Preferably, it also includes a fan driving module for driving a fan, and the fan driving control processor is electrically connected.

[0024] Preferably, it also includes a host, which is electrically connected to the control processor via a communication interface.

[0025] Preferably, it also includes a power supply system module, which is electrically connected to the switching power supply and the temperature control system for the single molecule immunoassay analyzer and is used to supply power to the temperature control system for the single molecule immunoassay analyzer.

[0026] Compared with the prior art, the present invention has the following beneficial effects:

[0027] The present invention incorporates the control of ambient temperature into the design of overall temperature control, thereby solving the problem that the temperature control effect of the instrument is greatly affected by changes in the external ambient temperature, thereby ensuring the effect of the actual incubation reaction. At the same time, in the case of special external disturbances, the system can perform rapid self-tuning to greatly reduce the incubation differences caused by temperature fluctuations. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 A schematic flow chart of a temperature control method for a single molecule immunoassay analyzer proposed by the present invention;

[0029] Figure 2 A schematic diagram of a temperature control device for a single molecule immunoassay analyzer proposed by the present invention;

[0030] Figure 3 This is a schematic diagram of a temperature control system for a single molecule immunoassay analyzer proposed in the present invention.

[0031] In the figure: 1. incubation chamber; 2. reaction cup; 3. space temperature sensor; 4. heated body temperature sensor; 5. heated body heater; 6. space heater; 7. fan; 8. control processor; 9. host; 10. power supply system module; 11. heater drive module; 12. fan drive module. DETAILED DESCRIPTION

[0032] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0033] At present, the entire experimental operation process of the single-molecule immunoassay detector is roughly as follows: place a disposable reaction cup into the reaction cup injection module → the transport module moves the reaction cup into the incubation module; add the patient's blood sample into the sample cup → the aspiration module extracts the sample and injects it into the reaction cup in the incubation module → the incubation module rotates to a position close to the reagent compartment module → the reagent adding module extracts the reagent in the reagent compartment module into the reaction cup of the incubation module → mixes and incubates for half an hour through the incubation module → the elution module performs elution → the incubation module rotates to a position close to the reagent compartment module → the reagent adding module extracts the reagent in the reagent compartment module into the reaction cup of the incubation module → mixes and incubates for half an hour through the incubation module → the elution module performs elution → diluent is added, sampling module samples → the detection module controls the distribution of the processed samples through the microfluidic chip, performs automatic focus in the detection device, records the number of fluorescent spots under the photo, and finally performs data analysis.

[0034] In single-molecule immunoassays, incubation usually needs to be carried out under specific temperature conditions to ensure the accuracy and reliability of the results. At the same time, changes in incubation temperature will also affect the speed and specificity of the immune response. The instrument usually needs to have good temperature stability to ensure that temperature changes during the reaction will not interfere with the results, thereby ensuring the consistency of the immune response. When the prior art is used, temperature control is only achieved through a temperature control device (heater, cooler or temperature control module), and a single heated body heater is used for temperature control. The actual temperature control effect will still be affected by changes in ambient temperature. At the same time, the common temperature control system has a long setting time period when it is subject to external interference, making it difficult to form rapid setting.

[0035] Based on the prior art, the present invention proposes a temperature control method, device and system for a single molecule immunoassay analyzer, which are as follows:

[0036] Reference Figure 1 , a temperature control method for a single molecule immunoassay analyzer, comprising the following steps:

[0037] S1, placing the reaction cup 2 on the heated body to form direct contact with the heated body to form heat conduction;

[0038] S2, monitoring the temperature of the heated body through the heated body temperature sensor 4;

[0039] S3, monitoring the temperature of the space where the heated body is located through the space temperature sensor 3;

[0040] S4, reading the temperature values ​​of the heated body temperature sensor 4 and the space temperature sensor 3;

[0041] S5, the temperature value is sent to the control processor 8, and the control processor 8 controls the temperature of the heated body and the space temperature through the heater according to the temperature value.

[0042] In this embodiment, the reading time interval of the temperature values ​​of the heated body temperature sensor 4 and the space temperature sensor 3 is 0.1s.

[0043] In this embodiment, when controlling the temperature of the heated body and the space temperature by a heater according to the temperature value, it also includes controlling the temperature of the heated body and the space temperature by a cooler.

[0044] The present invention incorporates the control of ambient temperature into the design of overall temperature control, thereby solving the problem that the temperature control effect of the instrument is greatly affected by changes in the external ambient temperature, thereby ensuring the effect of the actual incubation reaction. At the same time, in the case of special external disturbances, the system can perform rapid self-tuning to greatly reduce the incubation differences caused by temperature fluctuations.

[0045] In another embodiment, a temperature control method for a single molecule immunoassay analyzer comprises the following steps:

[0046] S1, placing the reaction cup 2 on the heated body to form direct contact with the heated body to form heat conduction;

[0047] S2, monitoring the temperature of the heated body through the heated body temperature sensor 4;

[0048] S3, monitoring the temperature of the space where the heated body is located through the space temperature sensor 3;

[0049] S4, reading the temperature values ​​of the heated body temperature sensor 4 and the space temperature sensor 3;

[0050] S5, the temperature value is sent to the control processor 8, and the control processor 8 controls the temperature of the heated body and the space temperature through the heater according to the temperature value.

[0051] In this embodiment, the reading time interval of the temperature values ​​of the heated body temperature sensor 4 and the space temperature sensor 3 is 0.2s or 0.3s.

[0052] In this embodiment, when controlling the temperature of the heated body and the space temperature by a heater according to the temperature value, it also includes controlling the temperature of the heated body and the space temperature by a cooler.

[0053] The present invention incorporates the control of ambient temperature into the design of overall temperature control, thereby solving the problem that the temperature control effect of the instrument is greatly affected by changes in the external ambient temperature, thereby ensuring the effect of the actual incubation reaction. At the same time, under the condition of special external disturbances, the system can perform rapid self-tuning to greatly reduce the incubation differences caused by temperature fluctuations, and the extension of the time interval can reduce the reading frequency and save energy consumption.

[0054] It should be noted that, for the aforementioned method embodiments, for the sake of simplicity, they are all described as a series of action combinations, but those skilled in the art should be aware that the present disclosure is not limited by the order of the actions described, because according to the present disclosure, certain steps can be performed in other orders or simultaneously. Secondly, those skilled in the art should also be aware that the embodiments described in the specification are all optional embodiments, and the actions and modules involved are not necessarily required by the present disclosure.

[0055] The above is an introduction to the method embodiment. The following is a further explanation of the scheme disclosed in the present invention through an apparatus embodiment.

[0056] See also Figure 2 A temperature control device for a single molecule immunoassay analyzer is used to implement a temperature control method for a single molecule immunoassay analyzer, including an incubation chamber 1, which is box-shaped, a heat receiving body is arranged inside the incubation wall, a placement groove is arranged on the top of the heat receiving body, the placement groove is adapted to a reaction cup 2, a heat receiving body temperature sensor 4 and a heat receiving body heater 5 are arranged inside the heat receiving body, the heat receiving body temperature sensor 4 and the heat receiving body heater 5 form heat conduction with the heat receiving body, and the heat receiving body material can be copper, and a space heater 6 and a space temperature sensor 3 are also arranged inside the incubation chamber 1.

[0057] By setting the heated body temperature sensor 4 and the heated body heater 5, the space heater 6 and the space temperature sensor 3, it is convenient to monitor the heated body temperature of the reaction cup 2 and the space temperature at the same time when performing single-molecule immunoassay, and the heated body temperature sensor 4 and the space temperature sensor 3 are used to monitor the heated body temperature and the internal temperature of the incubation space in real time, and the temperature is adjusted in real time by the heated body heater 5 and the space heater 6 according to the temperature parameters. Compared with the prior art, the present invention incorporates the control of ambient temperature into the design of overall temperature control, solves the problem that the temperature control effect of the instrument is greatly affected by the change of the external ambient temperature, thereby ensuring the effect of the actual incubation reaction. At the same time, in the case of special external disturbances, the system can perform rapid self-tuning to greatly reduce the incubation differences caused by temperature fluctuations.

[0058] In this embodiment, the heated body heater 5 and the space heater 6 are both electric heaters, and the electric heaters may be electric heating wires, electric heating tubes, and the like.

[0059] In this embodiment, a fan 7 is provided at the bottom of the space heater 6. The fan 7 is installed at the bottom of the incubation cavity 1 and is connected to the outside of the incubation cavity 1. When the space heater 6 is not started, cool air can be blown to reduce the temperature in the incubation cavity 1. When the space heater 6 is started, hot air can be blown to promote uniform temperature in the incubation cavity 1, which is convenient for further regulating the temperature inside the incubation space and provides stronger operability for temperature control.

[0060] See also Figure 3 A temperature control system for a single molecule immunoassay analyzer is used in a temperature control device for a single molecule immunoassay analyzer, including a control processor 8 and a heater driving module 11. The heated body temperature sensor 4 and the space temperature sensor 3 are both electrically connected to the control processor 8 through a detection conditioning circuit (the detection conditioning circuit converts the measured signal into a standard signal that can be recognized by the control processor 8 through operations such as amplification and filtering), and the heater driving module 11 is electrically connected to the heated body heater 5, the space heater 6 and the control processor 8.

[0061] When performing single-molecule immunoassay, the temperature of the heated body in which the reaction cup 2 is located and the space temperature are monitored at the same time. The control processor 8 reads the values ​​of the heated body temperature sensor 4 and the space temperature sensor 3 at certain time intervals, and controls the heater drive module 11 to control the heated body heater 5 and the space heater 6 to adjust the temperature in real time through the control processor 8 according to the temperature parameters. Compared with the prior art, the present invention incorporates the control of ambient temperature into the design of the overall temperature control, thereby solving the problem that the temperature control effect of the instrument is greatly affected by changes in the external ambient temperature, thereby ensuring the effect of the actual incubation reaction. At the same time, in the case of special external disturbances, the system can perform rapid self-tuning to greatly reduce the incubation differences caused by temperature fluctuations.

[0062] In this embodiment, a fan driving module 12 is also included, which is used to drive the fan 7. The fan 7 is electrically connected to the driving control processor 8. The fan 7 is installed at the bottom of the incubation chamber 1 and is connected to the outside of the incubation chamber 1. When the space heater 6 is not started, cool air can be blown to reduce the temperature in the incubation chamber 1. When the space heater 6 is started, hot air can be blown to promote uniform temperature in the incubation chamber 1, which is convenient for further regulating the temperature inside the incubation space and provides stronger operability for temperature control.

[0063] In the present embodiment, a host 9 is also included, and the host 9 is electrically connected to the control processor 8 through a communication interface. In specific implementation, the host 9 first issues a start instruction, and reads the temperature values ​​of the heated body temperature sensor 4 and the space temperature sensor 3 through the control processor 8. When the temperature value is lower than a certain value, the heated body heater 5 and the space heater 6 are started through the heater driving module 11 to heat the heated body and the incubation space respectively, and the temperature inside the heated body and the incubation space is adjusted at the same time. When the temperature is higher than a certain value, the heated body heater 5 and the space heater 6 may not be started, and the fan 7 may be used to blow air to reduce the temperature in the incubation space. The reading interval is 0.1s, which can achieve precise temperature control. When the temperature is within a certain value range, the current heating power can be maintained, and heating can be maintained in the current state to maintain temperature stability.

[0064] In this embodiment, a power supply system module 10 is further included. The power supply system module 10 is electrically connected to the switching power supply and the temperature control system for the single molecule immunoassay analyzer, and is used to supply power to the temperature control system for the single molecule immunoassay analyzer.

[0065] Various embodiments of the systems and techniques described above herein can be implemented in digital electronic circuit systems, integrated circuit systems, field programmable gate arrays (FPGAs), application specific integrated circuits (ASICs), application specific standard products (ASSPs), systems on chips (SOCs), load programmable logic devices (CPLDs), computer hardware, firmware, software, and / or combinations thereof. These various embodiments may include: being implemented in one or more computer programs, which may be executed and / or interpreted on a programmable system including at least one programmable processor, which may be a dedicated or general programmable processor, which may receive data and instructions from a storage system, at least one input device, and at least one output device, and transmit data and instructions to the storage system, the at least one input device, and the at least one output device.

[0066] The program code for implementing the method of the present disclosure may be written in any combination of one or more programming languages. These program codes may be provided to a processor or controller of a general-purpose computer, a special-purpose computer, or other programmable data processing device, so that the program code, when executed by the processor or controller, enables the functions / operations specified in the flow chart and / or block diagram to be implemented. The program code may be executed entirely on the machine, partially on the machine, partially on the machine and partially on a remote machine as a stand-alone software package, or entirely on a remote machine or server.

[0067] To provide interaction with a user, the systems and techniques described herein can be implemented on a computer having: a display device for displaying information to the user; and a keyboard and a pointing device (e.g., a mouse or trackball) through which the user can provide input to the computer. Other types of devices can also be used to provide interaction with the user; for example, the feedback provided to the user can be any form of sensory feedback (e.g., visual feedback, auditory feedback, or tactile feedback); and input from the user can be received in any form (including acoustic input, voice input, or tactile input).

[0068] The systems and techniques described herein may be implemented in a computing system that includes back-end components (e.g., as a data server), or a computing system that includes middleware components (e.g., an application server), or a computing system that includes front-end components (e.g., a user computer with a graphical user interface or a web browser through which a user can interact with implementations of the systems and techniques described herein), or a computing system that includes any combination of such back-end components, middleware components, or front-end components. The components of the system may be interconnected by any form or medium of digital data communication (e.g., a communications network). Examples of communications networks include: a local area network (LAN), a wide area network (WAN), and the Internet.

[0069] A computer system may include a client and a server. The client and the server are generally remote from each other and usually interact through a communication network. The relationship of client and server is generated by computer programs running on respective computers and having a client-server relationship with each other. The server may be a cloud server, a server of a distributed system, or a server combined with a blockchain.

[0070] It should be understood that the above-mentioned various forms of processes can be used to reorder, add or delete steps. For example, the steps recorded in this disclosure can be executed in parallel, sequentially, or in different orders, as long as the desired results of the technical solution of this disclosure can be achieved, and this document does not limit this.

[0071] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A temperature control method for a single molecule immunoassay analyzer, characterized in that: The following steps are involved: Place the reaction cup on the heated body to form direct contact with the heated body to achieve heat conduction; Monitor the temperature of the heated body by means of a heated body temperature sensor; Monitor the temperature of the space where the heated body is located through a space temperature sensor; Read the temperature values ​​of the heated body temperature sensor and the space temperature sensor; According to the temperature value, the temperature of the heated object and the space temperature are controlled by the heater.

2. The temperature control method for a single molecule immunoassay analyzer according to claim 1, characterized in that: The reading time interval of the temperature values ​​of the heated body temperature sensor and the space temperature sensor is 0.1s to 0.3s.

3. The temperature control method for a single molecule immunoassay analyzer according to claim 1, characterized in that: When controlling the temperature of the heated body and the space temperature by a heater according to the temperature value, it also includes controlling the temperature of the heated body and the space temperature by a cooler.

4. A temperature control device for a single molecule immunoassay analyzer, used to implement a temperature control method for a single molecule immunoassay analyzer according to any one of claims 1 to 3, characterized in that: It includes an incubation cavity, wherein a heat receiving body is arranged inside the incubation wall, a placement groove is arranged on the top of the heat receiving body, the placement groove is adapted to the reaction cup, a heat receiving body temperature sensor and a heat receiving body heater are arranged inside the heat receiving body, the heat receiving body temperature sensor and the heat receiving body heater form heat conduction with the heat receiving body, and a space heater and a space temperature sensor are also arranged inside the incubation cavity.

5. A temperature control device for a single molecule immunoassay analyzer according to claim 4, characterized in that: The heated body heater and the space heater are both electric heaters.

6. A temperature control device for a single molecule immunoassay analyzer according to claim 5, characterized in that: A fan is arranged at the bottom of the space heater, which can blow cool air to reduce the temperature in the incubation cavity when the space heater is not started, and can blow hot air to promote uniform temperature in the incubation cavity when the space heater is started.

7. A temperature control system for a single molecule immunoassay analyzer, applied to a temperature control device for a single molecule immunoassay analyzer according to any one of claims 4 to 6, characterized in that: It includes a control processor and a heater driving module. The heated body temperature sensor and the space temperature sensor are both electrically connected to the control processor. The heater driving module is electrically connected to the heated body heater, the space heater and the control processor.

8. A temperature control system for a single molecule immunoassay analyzer according to claim 7, characterized in that: It also includes a fan driving module for driving a fan, and the fan driving control processor is electrically connected.

9. A temperature control system for a single molecule immunoassay analyzer according to claim 8, characterized in that: It also includes a host, which is electrically connected to the control processor via a communication interface.

10. The temperature control system for a single molecule immunoassay analyzer according to claim 5, characterized in that: It also includes a power supply system module, which is electrically connected to the switch power supply and the temperature control system for the single molecule immunoassay analyzer and is used to supply power to the temperature control system for the single molecule immunoassay analyzer.

Images