A hydroponic nutrient solution for Leymus chinensis, its preparation method and application

Abstract

This invention discloses a hydroponic nutrient solution for Leymus chinensis, its preparation method, and its application, belonging to the field of plant cultivation technology. The nutrient solution comprises the following components per liter: ammonium nitrate 80-120 mg, sodium dihydrogen phosphate dihydrate 45-55 mg, potassium sulfate 80-90 mg, anhydrous calcium chloride 110-115 mg, magnesium sulfate heptahydrate 400-410 mg, manganese chloride tetrahydrate 1.5-2.0 mg, ammonium molybdate tetrahydrate 0.08-0.10 mg, boric acid 1.1-1.2 mg, zinc sulfate heptahydrate 0.02-0.05 mg, copper sulfate pentahydrate 0.02-0.05 mg, ferrous sulfate heptahydrate 15-35 mg, and disodium EDTA 20-50 mg. The hydroponic nutrient solution provided by this invention meets the nutritional requirements of Leymus chinensis. Seedlings grown using this nutrient solution grow rapidly and vigorously, with a significant increase in aboveground biomass and nutrient content, ensuring the high-quality forage characteristics of Leymus chinensis.

Description

Technical Field

[0001] This invention relates to the field of plant cultivation technology, specifically to a hydroponic nutrient solution for Leymus chinensis, its preparation method, and its application. Background Technology

[0002] Leymus chinensis (Trin. ex Bunge) Tzvelev, also known as alkali grass, is a perennial rhizomatous grass belonging to the genus Leymus in the family Poaceae. It exhibits strong adaptability to different growing environments and is one of the most salt-tolerant non-halophytes. It also possesses drought and cold resistance. Leymus chinensis is a superior forage and ecological grass in my country, boasting good palatability and high nutritional value. Its perennial nature and salt, drought, and cold resistance make it an important forage resource.

[0003] Hydroponics, a soilless cultivation method for plants, is short for nutrient solution culture. In hydroponics, plants are immobilized in a nutrient solution, which provides water and nutrients instead of soil. With appropriate temperature, humidity, and photoperiod controlled, the plants grow normally and complete their life cycle. Compared to soil cultivation, hydroponics exhibits significant advantages: the plant roots are completely immersed in the nutrient solution, inducing vigorous root growth and providing favorable conditions for biomass accumulation in the above-ground parts; hydroponics maximizes the absorption and utilization of nutrients in the nutrient solution, increasing yield and thus improving economic benefits; hydroponics allows for automated control of cultivation conditions and real-time monitoring of plant growth, saving labor costs; from an ecological perspective, hydroponics not only reduces soil fertility degradation but also minimizes the adverse environmental impacts of pesticides and fertilizers. Therefore, developing hydroponic technology suitable for Leymus chinensis growth holds promise for solving the current problems of insufficient yield and quality in Leymus chinensis populations.

[0004] The preparation of hydroponic nutrient solutions is crucial for obtaining high-yield, high-quality forage. For example, Hoagland's solution is one of the most commonly used formulations for plant nutrient solutions, beneficial for plant reproduction and growth. Patent document CN106810384A discloses a nutrient solution suitable for hydroponics of forage and other crops requiring less fertilizer during their growth period. Per 1000mL, it consists of the following components: 130mg urea, 20mg potassium dihydrogen phosphate, 60mg potassium chloride, 40mg magnesium sulfate, 2mg zinc sulfate, 3.5mg copper chloride, 4mg ferrous sulfate, 1.5mg manganese sulfate, 2mg calcium chloride, 2.5mg borax, 0.02mg sodium molybdate, and 2mL rare earth nutrient solution, with the remainder being water. Patent document CN117964426A discloses a hydroponic nutrient solution for cultivating forage germplasm resources, which is made of the following components: calcium nitrate tetrahydrate, potassium nitrate, ammonium dihydrogen phosphate, magnesium sulfate heptahydrate, ferric disodium EDTA, boric acid, manganese sulfate, copper sulfate, zinc sulfate, sodium molybdate, and compound fermentation liquid. This nutrient solution can promote plant growth and prevent root rot when used for the cultivation of seedlings of ice grass, oats, and ryegrass.

[0005] Different types of plants have different nutritional requirements. To adapt to the hydroponic technology of sheepgrass, it is necessary to optimize the formula of the hydroponic nutrient solution. Summary of the Invention

[0006] The purpose of this invention is to provide a hydroponic nutrient solution and hydroponic method suitable for the growth of Leymus chinensis, especially during the seedling to tillering stage, so as to promote the yield and quality of Leymus chinensis.

[0007] To achieve the above objectives, the present invention adopts the following technical solution:

[0008] This invention provides a hydroponic nutrient solution for Leymus chinensis, wherein each liter of the nutrient solution comprises: 80-120 mg ammonium nitrate, 45-55 mg sodium dihydrogen phosphate dihydrate, 80-90 mg potassium sulfate, 110-115 mg anhydrous calcium chloride, 400-410 mg magnesium sulfate heptahydrate, 1.5-2.0 mg manganese chloride tetrahydrate, 0.08-0.10 mg ammonium molybdate tetrahydrate, 1.1-1.2 mg boric acid, 0.02-0.05 mg zinc sulfate heptahydrate, 0.02-0.05 mg copper sulfate pentahydrate, 15-35 mg ferrous sulfate heptahydrate, and 20-50 mg disodium EDTA, with a pH value of 5.0-5.5.

[0009] The formula provided by this invention primarily provides nitrogen from ammonium nitrate, phosphorus from sodium dihydrogen phosphate, and potassium from potassium sulfate, offering essential macronutrients for the growth and development of Leymus chinensis and ensuring the accumulation of nutrients in the aboveground parts of the grass. The addition of calcium chloride, magnesium sulfate, and potassium sulfate ensures a normal supply of calcium, magnesium, and sulfur during Leymus chinensis development. Research results show that Leymus chinensis cultivated using the hydroponic nutrient solution provided by this invention exhibits a preference for aboveground biomass and nutrient accumulation, significantly improving yield and forage quality.

[0010] Preferably, each liter of nutrient solution contains the following components: ammonium nitrate 110-120 mg, sodium dihydrogen phosphate dihydrate 48-52 mg, potassium sulfate 85-90 mg, anhydrous calcium chloride 110-112 mg, magnesium sulfate heptahydrate 400-405 mg, manganese chloride tetrahydrate 1.8-1.9 mg, ammonium molybdate tetrahydrate 0.08-0.10 mg, boric acid 1.1-1.2 mg, zinc sulfate heptahydrate 0.04-0.05 mg, copper sulfate pentahydrate 0.03-0.04 mg, ferrous sulfate heptahydrate 30-35 mg, and disodium ethylenediaminetetraacetate 45-50 mg.

[0011] More preferably, each liter of nutrient solution contains the following components: ammonium nitrate 114.25 mg, sodium dihydrogen phosphate dihydrate 50.375 mg, potassium sulfate 89.25 mg, anhydrous calcium chloride 110.75 mg, magnesium sulfate heptahydrate 405 mg, manganese chloride tetrahydrate 1.875 mg, ammonium molybdate tetrahydrate 0.093 mg, boric acid 1.168 mg, zinc sulfate heptahydrate 0.044 mg, copper sulfate pentahydrate 0.039 mg, ferrous sulfate heptahydrate 34.75 mg, and disodium ethylenediaminetetraacetate 46.5 mg.

[0012] Compared to commonly used hydroponic nutrient solutions (Hogland solution), sheepgrass cultured using the above-mentioned nutrient solution showed a significant increase in plant height, which remained dominant, a significant decrease in root length increase, and a significant increase in aboveground biomass and nutrient content.

[0013] This invention also provides a method for preparing the above-mentioned hydroponic nutrient solution for Leymus chinensis, comprising the following steps:

[0014] (1) Dissolve ammonium nitrate, sodium dihydrogen phosphate dihydrate, potassium sulfate, anhydrous calcium chloride, and magnesium sulfate heptahydrate in water to prepare mother liquor I, mother liquor II, mother liquor III, mother liquor IV, and mother liquor V, respectively, for later use; mix manganese chloride tetrahydrate, ammonium molybdate tetrahydrate, boric acid, zinc sulfate heptahydrate, and copper sulfate pentahydrate and dissolve in water to prepare mother liquor VI, for later use; add disodium ethylenediaminetetraacetate to boiling water until completely dissolved, then add ferrous sulfate heptahydrate, and heat and stir until the solution turns brownish-yellow to prepare mother liquor VII, for later use;

[0015] (2) Take the proportions of Mother Solution I, Mother Solution II, Mother Solution III, Mother Solution IV, Mother Solution V, Mother Solution VI and Mother Solution VII, mix them, add water to make up the volume, and adjust the pH value to 5.0-5.5 with concentrated hydrochloric acid to obtain the above-mentioned Shepherd's Shoewort hydroponic nutrient solution.

[0016] Preferably, in step (1), the concentrations of mother liquor I, mother liquor II, mother liquor III, mother liquor IV, and mother liquor V are 91.4 g / L, 40.3 g / L, 71.4 g / L, 88.6 g / L, and 324 g / L, respectively; the concentrations of manganese dichloride tetrahydrate, ammonium molybdate tetrahydrate, boric acid, zinc sulfate heptahydrate, and copper sulfate pentahydrate in mother liquor VI are 1.5 g / L, 0.074 g / L, 0.934 g / L, 0.035 g / L, and 0.031 g / L; the concentrations of ferrous sulfate heptahydrate and disodium ethylenediaminetetraacetate in mother liquor VII are 6.95 g / L and 9.306 g / L; in step (2), mother liquors I-VI are mixed with mother liquor VII at a volume ratio of 1:4 and dissolved in water.

[0017] In this invention, the hydroponic nutrient solution for Leymus chinensis should be prepared and used immediately to prevent sedimentation from forming after prolonged storage, which would affect the cultivation effect.

[0018] This invention demonstrates that lowering the pH value can promote the dissolution of elements such as magnesium and calcium ions, and a pH value below 6 helps plants absorb nutrients.

[0019] This invention utilizes the aforementioned nutrient solution to cultivate Leymus chinensis, significantly increasing its biomass and ensuring its high-quality forage characteristics. This is of great significance for expanding forage cultivation and protecting the grassland ecosystem.

[0020] This invention provides the application of the aforementioned Leymus chinensis hydroponic nutrient solution as a hydroponic nutrient solution for the seedling and / or tillering stages of Leymus chinensis. The Leymus chinensis hydroponic nutrient solution provided by this invention meets the nutrient requirements of Leymus chinensis during hydroponic cultivation, especially for seedlings. Leymus chinensis seedlings using the nutrient solution of this invention grow rapidly and vigorously.

[0021] The present invention also provides a hydroponic method for Leymus chinensis, the method comprising: transplanting the germinated Leymus chinensis seedlings into a hydroponic facility containing the Leymus chinensis hydroponic nutrient solution, wherein the hydroponic conditions are: 22-26℃, 16h light / 8h darkness; and changing the nutrient solution once a week.

[0022] As a preferred method, the cultivation method for Leymus chinensis seedlings includes: selecting plump Leymus chinensis seeds, removing the husks, inoculating them on 1 / 2 MS medium, and maintaining them at 22-26℃ with 16 hours of light / 8 hours of darkness until the seedlings grow to about 7-10 cm.

[0023] Transplant seedlings once they have grown to a certain size to ensure they have sufficient growing space. Preferably, transplant them into hydroponic systems at a density of 0.05 seedlings / cm². 2 Specifically, add one liter of nutrient solution to an 8cm×12cm×11cm hydroponic device.

[0024] Preferably, the hydroponic facility is made of opaque material, and the nutrient solution is partially shielded from light to prevent algae growth.

[0025] The beneficial effects of this invention are as follows:

[0026] (1) The hydroponic nutrient solution provided by this invention has a reasonable composition that meets the nutritional requirements of Leymus chinensis during its growth, especially from the seedling stage to the tillering stage. Leymus chinensis seedlings using the nutrient solution of this invention grow rapidly and vigorously, with a significant increase in aboveground biomass and nutrient content, ensuring the high-quality forage characteristics of Leymus chinensis. Using the nutrient solution of this invention can reduce the use of chemical fertilizers and pesticides, playing a positive role in protecting the ecological balance.

[0027] (2) The hydroponic technology provided by the present invention can expand the planting range of sheep grass, accelerate the growth of sheep grass, improve the production quality of sheep grass, and reduce manual operation and its costs; the hydroponic method can avoid the occurrence of soil-borne diseases and pests, and ensure the safety of the environment for the growth of sheep grass. Attached Figure Description

[0028] Figure 1 Seedlings of Leymus chinensis 7 days after seed germination.

[0029] Figure 2 The growth of Leymus chinensis in hydroponics for 0-1 weeks in different embodiments.

[0030] Figure 3 The growth of Leymus chinensis in hydroponics for 2-5 weeks in different implementation examples.

[0031] Figure 4 The growth of Leymus chinensis in hydroponics for 6-8 weeks in different embodiments.

[0032] Figure 5 Statistical analysis of the growth trend and plant height increase of Leymus chinensis in hydroponics for 1-8 weeks in different embodiments.

[0033] Figure 6 Statistical analysis of root length growth trends of Leymus chinensis grown hydroponically for 1-8 weeks in different embodiments.

[0034] Figure 7 The accumulation of nutrients in Leymus chinensis after 8 weeks of hydroponics in different embodiments is shown. A represents neutral detergent fiber (NDF, %), and B represents acid detergent fiber (ADN, %). Detailed Implementation

[0035] The present invention will be further described below with reference to specific embodiments. These embodiments are for illustrative purposes only and are not intended to limit the scope of the invention. Any modifications or substitutions made to the methods, steps, or conditions of the present invention without departing from the spirit and essence of the invention are within the scope of the invention.

[0036] Unless otherwise specified, the experimental methods used in the following examples are conventional methods; the materials and reagents used are commercially available unless otherwise specified.

[0037] The seeds of Leymus chinensis in the following examples were obtained from the Sharqin Breeding Base in Hohhot. 1 / 2 MS medium was purchased from Coolaber, catalog number PM1011.

[0038] Example 1: Formula of Sheepgrass hydroponic nutrient solution A stock solution

[0039] The formula for the hydroponic nutrient solution A stock solution of Leymus chinensis is as follows:

[0040] Mother liquor I: Dissolve 64g NH4NO3 in water and bring the volume to 1L;

[0041] Mother liquor II: Dissolve 40.3g NaH2PO4·2H2O in water and bring the volume up to 1L;

[0042] Mother liquor III: 71.4g K2SO4 dissolved in water, diluted to 1L;

[0043] Mother liquor IV: Dissolve 88.6g of anhydrous CaCl2 in water and bring the volume to 1L;

[0044] Mother liquor V: 324g MgSO4·7H2O dissolved in water, diluted to 1L;

[0045] Mother liquor VI: 1.5g MnCl2·4H2O, 0.074g (NH4)6Mo7O 24 Dissolve 4H2O, 0.934g H3BO3, 0.035g ZnSO4·7H2O, and 0.031g CuSO4·5H2O in water and bring the volume to 1L.

[0046] Mother liquor VII: 3.5g FeSO4·7H2O and 4.7g EDTA-Na2 dissolved in water, and diluted to 1L.

[0047] Note: When preparing stock solution VII, first add EDTA-Na2 to boiling ddH2O until completely dissolved, then add FeSO4·7H2O, and heat while stirring continuously (2-3 hours) until the solution turns brownish-yellow.

[0048] Example 2: Formula of Sheepgrass hydroponic nutrient solution B stock solution

[0049] The difference between the formula of the hydroponic nutrient solution B for *Leymus chinensis* and that of Example 1 is that in the latter, 6.95g of FeSO4·7H2O and 9.306g of EDTA-Na2 are dissolved in water and brought to a final volume of 1L. All other components are the same as in Example 1.

[0050] Example 3: Formula of Sheepgrass hydroponic nutrient solution C stock solution

[0051] The difference between the stock solution formula of Leymus chinensis hydroponic nutrient solution C and Example 1 is that stock solution I: 91.4g NH4NO3 dissolved in water and diluted to 1L; stock solution VII: 6.95g FeSO4·7H2O and 9.306g EDTA-Na2 dissolved in water and diluted to 1L. All other aspects are the same as in Example 1.

[0052] Example 4: Preparation of hydroponic nutrient solution for Leymus chinensis

[0053] Experimental group: Hydroponic nutrient solutions for Leymus chinensis were prepared using the mother liquors from Examples 1-3. The method was as follows: 5 mL each of mother liquor I-VI and 20 mL of mother liquor VII were added to every 4 L of hydroponic nutrient solution. Water was added to bring the volume to 4 L and the solution was mixed thoroughly. The pH value was adjusted to 5.0-5.5 with concentrated hydrochloric acid.

[0054] Control group: Hogland nutrient solution, purchased from Phygene, catalog number PH1782. Each liter of nutrient solution contains 607 mg potassium sulfate, 115 mg ammonium dihydrogen phosphate, 493 mg magnesium sulfate, 20 mg sodium EDTA iron, 2.86 mg ferrous sulfate, 4.5 mg borax, 2.13 mg manganese sulfate, 0.05 mg copper sulfate, 0.22 mg zinc sulfate, 0.02 mg ammonium sulfate, and calcium salts.

[0055] Example 5: Hydroponic cultivation of Leymus chinensis

[0056] 1. The detailed steps for hydroponic cultivation of Leymus chinensis are as follows:

[0057] (1) Selection of equipment for sheepgrass cultivation: black hydroponic boxes and opaque hydroponic troughs are used for hydroponics. Black floats are made to prevent the subsequent growth of algae and affect the growth of plants.

[0058] (2) Seed treatment of Leymus chinensis: Select plump seeds with good grain condition, remove the husks, and germinate them on 1 / 2 MS medium under the following conditions: 26℃, 16h light / 8h dark. Seedlings were observed 7 days after germination. Figure 1 As shown.

[0059] (3) The germinated seedlings were transplanted into the hydroponic nutrient solutions prepared in Example 4, with a transplanting density of 0.05 seedlings / cm². 2 The cultivation conditions are: 26℃, 16h light / 8h darkness. The nutrient solution needs to be changed once a week.

[0060] (4) Twelve *Leymus chinensis* plants were cultivated in each example, and the growth of *Leymus chinensis* during 0-8 weeks was as follows: Figure 2-4 As shown in the figure. The height and root length of *Leymus chinensis* were measured weekly. The differences between the weekly measured height and root length and the initial values ​​during hydroponics were statistically analyzed to assess the growth in height and root length after hydroponics. The results are shown in the figure. Figure 5 , Figure 6 As shown, n = 12, **** represents P < 0.0001, *** represents P < 0.001, ** represents P < 0.01, and * represents P < 0.05.

[0061] (5) Samples were taken from the top 3 cm of the sheepgrass after 8 weeks of cultivation. After drying at 75℃ for 48 hours, the nutritional components, including neutral detergent fiber (NDF, %) and acid detergent fiber (ADF, %), were measured. The measurement method was referenced in (Zhang Liying. Feed Analysis and Feed Quality Testing Technology [M]. Beijing: China Agricultural University Press, 2016). The statistical results are as follows: Figure 7 As shown, n = 6, and *** represents P < 0.001.

[0062] 2. Results Analysis

[0063] Analysis of the growth trends of the four groups of Leymus chinensis revealed no significant difference in aboveground plant height among the different examples during the initial 1-5 weeks of hydroponics. After 6 weeks of hydroponics, the Leymus chinensis cultured in Example 3 showed a significantly increased plant height compared to the control group, and this advantage persisted. Furthermore, the increase in root length of the Leymus chinensis cultured in Example 3 was significantly less than in the other examples, indicating that the growth of the Leymus chinensis cultured in Example 3 was more focused on the aboveground parts, resulting in a significant increase in aboveground biomass.

[0064] Analysis of the aboveground nutrients of sheep grass cultivated in different embodiments also confirmed that the sheep grass cultivated in Example 3 had higher NDF and ADF accumulation, and better forage quality.

[0065] Therefore, the present invention provides a hydroponic nutrient solution formula for Leymus chinensis and its preparation method, with the optimal addition concentration of each component as shown in Example 3.

Claims

1. A method for hydroponic culture of Chinese wildrye (Leymus chinensis) at seedling stage to tillering stage or tillering stage, characterized in that, The method comprises transplanting the germinated Leymus chinensis seedlings into a hydroponic facility containing the Leymus chinensis hydroponic nutrient solution, and the hydroponic conditions are 22-26 DEG C, 16h light / 8h darkness, and the nutrient solution is replaced once a week. The composition of the Leymus chinensis hydroponic nutrient solution per liter includes 110-120 mg of ammonium nitrate, 48-52 mg of sodium dihydrogen phosphate dihydrate, 85-90 mg of potassium sulfate, 110-112 mg of calcium chloride anhydrous, 400-405 mg of magnesium sulfate heptahydrate, 1.8-1.9 mg of manganese chloride tetrahydrate, 0.08-0.10 mg of ammonium molybdate tetrahydrate, 1.1-1.2 mg of boric acid, 0.04-0.05 mg of zinc sulfate heptahydrate, 0.03-0.04 mg of copper sulfate pentahydrate, 30-35 mg of ferrous sulfate heptahydrate, and 45-50 mg of disodium ethylenediaminetetraacetate, and the pH value is 5.0-5.

5. The preparation method of the Leymus chinensis hydroponic nutrient solution comprises the following steps: (1) respectively dissolve ammonium nitrate, sodium dihydrogen phosphate dihydrate, potassium sulfate, calcium chloride anhydrous and magnesium sulfate heptahydrate in water to prepare mother liquor I, mother liquor II, mother liquor III, mother liquor IV and mother liquor V for standby; mix manganese chloride tetrahydrate, ammonium molybdate tetrahydrate, boric acid, zinc sulfate heptahydrate and copper sulfate pentahydrate in water to prepare mother liquor VI for standby; add disodium ethylenediaminetetraacetate into boiling water until completely dissolved, then add ferrous sulfate heptahydrate, and stir while heating until the solution is brownish yellow to prepare mother liquor VII for standby; (2) measure the mother liquor I, the mother liquor II, the mother liquor III, the mother liquor IV, the mother liquor V, the mother liquor VI and the mother liquor VII according to the proportion, mix and add water to constant volume, and adjust the pH value to 5.0-5.5 by using concentrated hydrochloric acid to obtain the Leymus chinensis hydroponic nutrient solution; The Leymus chinensis hydroponic nutrient solution is prepared on demand.

2. The method according to claim 1, wherein the water culture of the seedling stage to tillering stage or tillering stage of Chinese wildrye is characterized by, The composition of the Leymus chinensis hydroponic nutrient solution per liter includes 114.25 mg of ammonium nitrate, 50.375 mg of sodium dihydrogen phosphate dihydrate, 89.25 mg of potassium sulfate, 110.75 mg of calcium chloride anhydrous, 405 mg of magnesium sulfate heptahydrate, 1.875 mg of manganese chloride tetrahydrate, 0.093 mg of ammonium molybdate tetrahydrate, 1.168 mg of boric acid, 0.044 mg of zinc sulfate heptahydrate, 0.039 mg of copper sulfate pentahydrate, 34.75 mg of ferrous sulfate heptahydrate and 46.5 mg of disodium ethylenediaminetetraacetate.

3. The method according to claim 2, wherein the water culture of the seedling stage to tillering stage or tillering stage of Chinese wildrye is characterized by, In step (1), the concentrations of the mother liquor I, the mother liquor II, the mother liquor III, the mother liquor IV and the mother liquor V are 91.4 g / L, 40.3 g / L, 71.4 g / L, 88.6 g / L and 324 g / L respectively; the concentration of manganese chloride tetrahydrate in the mother liquor VI is 1.5 g / L, the concentration of ammonium molybdate tetrahydrate is 0.074 g / L, the concentration of boric acid is 0.934 g / L, the concentration of zinc sulfate heptahydrate is 0.035 g / L and the concentration of copper sulfate pentahydrate is 0.031 g / L; the concentration of ferrous sulfate heptahydrate in the mother liquor VII is 6.95 g / L and the concentration of disodium ethylenediaminetetraacetate is 9.306 g / L; in step (2), the mother liquor I-VI and the mother liquor VII are mixed in water according to the volume ratio of 1:

4.

4. The hydroponic method for Leymus chinensis from seedling stage to tillering stage or during tillering stage as described in claim 1, characterized in that, The method for cultivating the seedling of Leymus chinensis comprises the following steps: selecting the seed of Leymus chinensis with full grain to remove the hull, inoculating on 1 / 2MS culture medium, and culturing at 22-26 DEG C, 16h light / 8h darkness until the seedling grows to about 7-10cm.

5. The hydroponic method for Leymus chinensis from seedling stage to tillering stage or during tillering stage as described in claim 1, characterized in that, Seedling transplanting density was 0.05 plants / cm 2 .

6. The method for hydroponic culture of Chinese wildrye at seedling stage to tillering stage or tillering stage according to claim 1, characterized in that, The water culture facility adopts light-proof material.

Images