A composition containing 10-hydroxy-2-decenoic acid and its use

Abstract

This invention belongs to the field of cosmetic technology, specifically relating to a composition containing 10-hydroxy-2-decenoic acid and its application. The composition is obtained by compounding probiotic-fermented brown algae, pterostilbene, and 10-hydroxy-2-decenoic acid in a certain proportion. Experiments have shown that the components of the above composition can produce a synergistic effect, significantly inhibiting the production of inflammatory factors, resisting inflammatory aging, and inhibiting lipid peroxidation. Furthermore, this invention unexpectedly discovered that compounding probiotic-fermented brown algae, 10-hydroxy-2-decenoic acid, and pterostilbene can significantly improve the light and heat stability of pterostilbene, thus possessing good practical application value.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to a composition containing 10-hydroxy-2-decenoic acid and its application. Background Technology

[0002] The information disclosed in the background section of this invention is intended only to enhance the understanding of the overall background of the invention and is not necessarily to be construed as an admission or in any way implying that such information constitutes prior art known to those skilled in the art.

[0003] Franceschi first proposed the concept of "inflammatory aging" in 2000. Inflammatory stimuli primarily originate from endogenous, ectopic, or degenerated molecules produced by damaged and / or dead cells and organelles (cellular debris). These molecules are recognized by receptors of the innate immune system, inducing an inflammatory response. The body produces these molecules under normal physiological conditions, and this production gradually increases with age, while the clearance of these molecules by the proteasome through autophagy and / or mitochondrial autophagy gradually declines. This "autoimmune response" process promotes the occurrence or development of chronic diseases, thereby accelerating and aggravating the aging process both locally and systemically. Immunosenescence refers to the decline in adaptive immune responses and the dominance of innate immune responses with age. Its significant characteristic is the increase in pro-inflammatory mediators, with elevated serum levels of inflammatory factors such as interleukin (IL)6, IL-8, and tumor necrosis factor-α (TNF-α). Numerous studies have shown that immunosenescence is a contributing factor to inflammatory aging and is considered to share a common inflammatory basis with most age-related diseases.

[0004] Therefore, inflammation has been identified as a major target for anti-aging strategies. As the largest organ and outermost protective barrier in the human body, the skin most directly exhibits signs of aging, such as thinning, dryness, age spots, and fine lines. These signs of aging are accompanied by specific epigenetic modifications, reduced cell proliferation / tissue renewal, accumulation of senescent cells, changes in the extracellular matrix, and a pro-inflammatory environment conducive to disease or aging.

[0005] Lipid peroxidation refers to the oxidation and deterioration of polyunsaturated fatty acids, producing lipid peroxidation products. Free radicals attack the unsaturated fatty acids in the sebum membrane, damaging its components, impairing the skin barrier function, and reducing its ability to retain moisture, thus leading to dry skin. Lipid peroxidation disrupts membrane integrity and impairs its filtration and barrier functions, affecting cell structure and function. Furthermore, lipid peroxidation can trigger skin inflammation, severely impacting skin health and appearance. The mechanism involves the conversion of membrane lipids into potent pharmacologically active lipotoxins (LTs) and prostaglandins (PG2) under the action of lipoxygenases and reactive oxygen species, which then generate MDA under internal peroxidation. Both metabolic pathways produce oxygen free radicals, creating a cycle from reactive oxygen species to fatty acid oxidation and the regeneration of reactive oxygen species, amplifying the inflammatory response and further accelerating skin aging.

[0006] Royal jelly acid, also known as 10-hydroxy-2-decenoic acid (10-HDA), is an unsaturated fatty acid found in royal jelly. As a substance with various physiological activities, royal jelly acid has been explored and applied with great potential in cosmetics development and medical applications. Current research indicates that royal jelly acid possesses anti-aging, anti-inflammatory, whitening, and antioxidant properties. However, its relatively high price significantly limits its use. Summary of the Invention

[0007] To address the shortcomings of existing technologies, this invention provides a composition containing 10-hydroxy-2-decenoic acid and its applications. Specifically, this invention involves compounding probiotic-fermented brown algae, pterostilbene, and 10-hydroxy-2-decenoic acid in a certain proportion to obtain the composition. Experiments have shown that the components of the above composition can produce a synergistic effect, significantly inhibiting the production of inflammatory factors, resisting inflammatory aging, and inhibiting lipid peroxidation. Furthermore, this invention unexpectedly discovered that compounding probiotic-fermented brown algae, 10-hydroxy-2-decenoic acid, and pterostilbene also…

[0008] It can significantly improve the light and heat stability of Pterostilbene. Based on the above research results, this invention is thus completed.

[0009] To achieve the above-mentioned technical objectives, the present invention provides the following technical solution:

[0010] A first aspect of the present invention provides a composition containing 10-hydroxy-2-decenoic acid, said composition comprising the following components in parts by weight:

[0011] Probiotic fermented brown algae 0.1-50 parts, pterostilbene 0.001-0.5250 parts, 10-hydroxy-2-decenoic acid 0.001-2 parts.

[0012] A second aspect of the invention provides the use of the above-described composition in the preparation of cosmetics.

[0013] The cosmetic product has at least one or more of the following effects:

[0014] (a) Anti-wrinkle;

[0015] (b) Tightening;

[0016] (c) Anti-aging.

[0017] A third aspect of the invention provides a cosmetic product with anti-wrinkle, firming, and anti-aging effects, said cosmetic product comprising at least the above-described composition. More specifically, said cosmetic product can be a skin care product.

[0018] A fourth aspect of the present invention provides a method for preparing the above-mentioned cosmetic, the method comprising mixing the composition with other raw material components.

[0019] The beneficial technical effects of one or more of the above technical solutions are as follows:

[0020] The above-mentioned composition containing 10-hydroxy-2-decenoic acid can significantly inhibit the production of inflammatory factors and resist inflammatory aging. Furthermore, the components exhibit synergistic effects, reducing the amount of 10-hydroxy-2-decenoic acid required and lowering formulation costs. In addition, the above technical solution unexpectedly discovered that 10-hydroxy-2-decenoic acid also has a certain inhibitory effect on lipid peroxidation. The composition obtained by combining it with probiotic-fermented brown algae and pterostilbene exhibits a stronger inhibitory effect on lipid peroxidation, thus effectively expanding the efficacy range of 10-hydroxy-2-decenoic acid. Simultaneously, the above technical solution research found that combining probiotic-fermented brown algae, 10-hydroxy-2-decenoic acid, and pterostilbene can effectively improve the light and heat stability of pterostilbene, thus possessing good practical application value. Detailed Implementation

[0021] It should be noted that the following detailed description is illustrative and intended to provide further explanation of the invention. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains.

[0022] It should be noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the scope of exemplary embodiments according to the invention. As used herein, the singular form is intended to include the plural form as well, unless the context clearly indicates otherwise. Furthermore, it should be understood that when the terms "comprising" and / or "including" are used in this specification, they indicate the presence of features, steps, operations, devices, components, and / or combinations thereof.

[0023] The present invention will now be further illustrated with specific examples. These examples are for illustrative purposes only and do not limit the scope of the invention. Unless otherwise specified, experimental conditions not explicitly stated in the examples are generally performed under conventional conditions or as recommended by the reagent company. Unless otherwise specified, all reagents and consumables used in the following examples are commercially available.

[0024] In a typical embodiment of the present invention, a composition containing 10-hydroxy-2-decenoic acid is provided, the composition comprising the following components in parts by weight:

[0025] Probiotic fermented brown algae 0.1-50 parts, pterostilbene 0.001-0.5250 parts, 10-hydroxy-2-decenoic acid 0.001-2 parts.

[0026] The probiotic-fermented brown algae is a complex ingredient containing more than four components, including polysaccharides, oligosaccharides, amino acids, small peptides, and glutathione. It possesses multiple skincare benefits, such as regulating skin microecology, repairing, anti-wrinkle, and anti-oxidation. It is obtained through multi-stage fermentation using various food-grade probiotics. In one specific embodiment of this invention, the probiotic-fermented brown algae is specifically a seaweed fermentation liquid obtained based on the fermentation method described in CN 108653059 A. Further, the brown algae is kelp. Meanwhile, the probiotic-fermented brown algae (i.e., seaweed fermentation liquid) does not contain live bacteria.

[0027] Pterocarpus marsupium, an extract of the bark of the Pterocarpus macrocarpus tree, is a methylated derivative of resveratrol and is hailed as a new generation of resveratrol. It is widely distributed in plants such as sandalwood, blueberries, strawberries, grapes, and aloe vera. According to domestic and international research reports, pterocarpus marsupium possesses multiple skincare benefits, including whitening, anti-oxidation, anti-inflammation, anti-glycation, protection against UV damage, and firming and anti-wrinkle effects, making it considered one of the most versatile whitening and anti-aging cosmetic ingredients. To date, no other natural plant ingredient has been found to surpass its efficacy; therefore, pterocarpus marsupium is also known as the "King of Plant Whitening and Antioxidant." However, the inherent instability of pterocarpus marsupium greatly limits its application. This is because pterocarpus marsupium is a polyphenol with poor stability and is easily oxidized. When exposed to environmental conditions such as ultraviolet radiation, oxygen, high temperatures, and pro-oxidants, it is prone to degradation and isomerization reactions.

[0028] The 10-hydroxy-2-decenoic acid mentioned is royal jelly acid, and its source is not specifically limited.

[0029] The 10-hydroxy-2-decenoic acid can be a pure product, or it can be an aqueous solution, alcoholic solution, liposome, olactone, or cyclodextrin inclusion complex containing 10-hydroxy-2-decenoic acid. In one specific embodiment of the present invention, the 10-hydroxy-2-decenoic acid is an aqueous solution containing 10-hydroxy-2-decenoic acid, with a mass fraction (w / w) of 1-10%, more preferably 5%.

[0030] In another specific embodiment of the present invention, the composition comprises the following components in parts by weight:

[0031] Five parts of probiotic-fermented brown algae, 0.1 parts of pterostilbene, and 0.1 parts of 10-hydroxy-2-decenoic acid.

[0032] In another specific embodiment of the present invention, the use of the above composition in the preparation of cosmetics is provided.

[0033] The cosmetic product has at least one or more of the following effects:

[0034] (a) Anti-wrinkle;

[0035] (b) Tightening;

[0036] (c) Anti-aging.

[0037] In application (c), the anti-aging effect is specifically manifested in inhibiting lipid peroxidation of the skin, thereby achieving the anti-aging effect.

[0038] In another specific embodiment of the present invention, a cosmetic product with anti-wrinkle, firming, and anti-aging effects is provided, wherein the cosmetic product comprises at least the above-described composition. More specifically, the cosmetic product may be a skin care product.

[0039] According to the cosmetic of the present invention, the amount of the composition added is 0.5-10% based on the total mass of the cosmetic, preferably 1-5%, such as 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9% or 10%.

[0040] The cosmetics may also contain any other ingredients permitted in the cosmetics field, including but not limited to emulsifiers, emollients, humectants, thickeners, and preservatives.

[0041] Meanwhile, by reasonably adding the above-mentioned raw material components, the present invention can also prepare different cosmetic dosage forms, such as aqueous solutions, emulsions, ointments, etc. Furthermore, based on the above-mentioned basic cosmetic categories, other cosmetic categories can be further derived and prepared, such as cleansing creams, facial cleansers, face creams, toners, face masks, etc., which are not specifically limited here.

[0042] In another specific embodiment of the present invention, a method for preparing the above-mentioned cosmetic is provided, the method comprising mixing the composition with other raw material components.

[0043] The following examples further illustrate the present invention, but do not constitute a limitation thereof. It should be understood that these examples are for illustrative purposes only and not for limiting the scope of the invention. Test methods in the following examples, unless otherwise specified, are generally performed under conventional conditions. The probiotic fermented brown algae in the embodiments and comparative examples of the present invention are the seaweed fermentation broth obtained using the preparation method of Example 1 in CN 108653059 A.

[0044] The specific components of Examples 1 to 6 are shown in the table below:

[0045]

[0046]

[0047] The specific compositions of comparative examples 1 to 7 are shown in the table below:

[0048]

[0049] Application Example 1: Emulsion Preparation

[0050] Phase A (parts by weight): 1 part A165 (emulsifier), 3 parts GTCC, 3 parts white oil, 0.5 parts cetearyl alcohol, 5 parts silicone oil, 0.1 part pterostilbene (pure powder);

[0051] Phase B (parts by weight): 4 parts glycerin, 4 parts butylene glycol, 0.4 parts EMT-10 (thickener), 0.1 parts xanthan gum, 0.4 parts succinate, 0.4 parts hexanediol, 0.2 parts soybean lecithin, 100 parts deionized water;

[0052] Phase C (parts by weight): 2 parts of 10-hydroxy-2-decenoic acid aqueous solution (5% by mass) and 5 parts of probiotic fermented brown algae.

[0053] The emulsion preparation steps are as follows:

[0054] 1) First, disperse EMT-10, xanthan gum and soybean lecithin into glycerol and butylene glycol, then add acetone, hexanediol and deionized water, heat and stir in an 80°C water bath to fully dissolve them, and obtain phase B;

[0055] 2) Melt 0.1 parts of A165, GTCC, white oil, cetearyl alcohol, silicone oil, and pterostilbene (pure powder) in a water bath at 83°C to obtain phase A;

[0056] 3) Heat phase A and phase B in a water bath at 83°C. When the two phases are at the same temperature, slowly add phase A to phase B while stirring phase B at 500 r / min.

[0057] 4) After phase B and phase A are mixed, the emulsion is cooled while stirring at 430 r / min.

[0058] 5) When the emulsion temperature drops to 40-45℃, add phase C to the system and stir at 60 r / min to cool it down;

[0059] 6) When the temperature drops to 38°C, static cooling is performed to obtain an emulsion containing the composition of Example 1.

[0060] Application Example 2: Cream Preparation

[0061] Phase A (parts by weight): A165 (emulsifier) ​​2.5 parts, GTCC 3 parts, white oil 3 parts, cetearyl alcohol 1.5 parts, shea butter 2 parts, silicone oil 5 parts, pterostilbene (pure powder) 0.1 parts;

[0062] Phase B (parts by weight): 4 parts glycerin, 4 parts butylene glycol, 0.4 parts EMT-10 (thickener), 0.4 parts sodium polyacrylate, 0.1 parts xanthan gum, 0.4 parts succinate, 0.4 parts hexanediol, 0.2 parts soybean lecithin, and 100 parts deionized water;

[0063] Phase C (parts by weight): 2 parts of 10-hydroxy-2-decenoic acid aqueous solution (5% by mass) and 5 parts of probiotic fermented brown algae.

[0064] The emulsion preparation steps are as follows:

[0065] 1) First, disperse EMT-10, sodium polyacrylate, xanthan gum and soybean lecithin into glycerol and butylene glycol, then add acetone, hexanediol and deionized water, heat and stir in an 80°C water bath to fully dissolve them, and obtain phase B;

[0066] 2) Melt 0.1 parts of A165, GTCC, white oil, cetearyl alcohol, silicone oil, shea butter, and pterostilbene (pure powder) in a water bath at 83°C to obtain phase A;

[0067] 3) Heat phase A and phase B in a water bath at 83°C. When the two phases are at the same temperature, slowly add phase A to phase B while stirring phase B at 500 r / min.

[0068] 4) After phase B and phase A are mixed, the emulsion is cooled while stirring at 430 r / min.

[0069] 5) When the emulsion temperature drops to 40-45℃, add phase C to the system and stir at 60 r / min to cool it down;

[0070] 6) When the temperature drops to 38°C, the cream containing the composition of Example 1 is obtained by static cooling.

[0071] Experiment Example 1: Inhibition of Inflammatory Factor TNF-α

[0072] 1) The specific experimental methods for TNF-α detection are shown in Table 1 below:

[0073] Table 1

[0074]

[0075] Note: The sample solution was prepared by using dimethyl sulfoxide (DMSO) to prepare a solution with a total mass of 100g for both the example and comparative examples.

[0076] 2) Preparation of working solution for positive control group (dexamethasone): Dissolve 2 μL of 10% stock solution in 2 mL of culture medium to prepare 0.01% dexamethasone.

[0077] 3) Cell seeding: at a rate of 2.8 × 10⁻⁶ 5 Cells were seeded at a density of cells per well into 6-well plates and incubated overnight in an incubator (37°C, 5% CO2).

[0078] 4) Drug administration: According to the experimental design in Table 1, when the cell deposition rate in the 6-well plate reached 40%–60%, the cells were administered to groups, with 2 mL of sample added to each well, and each group had 3 replicates. After drug administration, the 6-well plates were placed in an incubator (37℃, 5% CO2) for 24 h.

[0079] 5) UVB irradiation: After washing cells with PBS, according to the experimental groups, the groups receiving UVB irradiation were subjected to 300 mJ / cm² irradiation. 2 UVB irradiation.

[0080] 6) Post-incubation: After washing the cells with PBS, place the 6-well plate in an incubator (37℃, 5% CO2) for 24 hours for post-incubation.

[0081] 7) Collect cell supernatant: After incubation for 24 hours, collect the cell culture supernatant into EP tubes and store them in a -80℃ freezer.

[0082] Store frozen.

[0083] 8) ELISA detection: Perform detection and analysis according to the instructions of the ELISA detection kit.

[0084] 9) Calculation of TNF-α inhibition rate: The results are shown in Table 2 below:

[0085] Table 2. TNF-α inhibition rate of each group

[0086]

[0087]

[0088] Note: Significance is indicated by *, P-value < 0.05 is indicated by *, and P-value < 0.01 is indicated by **.

[0089] Test results show that the composition of the present invention can significantly inhibit the production of TNF-α, and the components have a certain synergistic effect, reducing the skin damage caused by inflammatory aging.

[0090] Experimental Example 2: Lipid Peroxidation – Malondialdehyde (MDA) Inhibition Detection

[0091] 1) Cell seeding: After cell resuscitation, when the cell plating rate reaches about 60%, seed the cells into 6-well plates and incubate overnight in a CO2 incubator (37℃, 5% CO2).

[0092] 2) Solution preparation: Prepare working solutions of the test substances according to the test groups, as shown in Table 3 below.

[0093] Table 3. Specific experimental design for lipid peroxidation detection.

[0094]

[0095]

[0096] 3) Drug administration: According to the test groups, when the cell deposition rate in the 6-well plate reached 30%–50%, drug administration was performed in groups, with 3 replicates per group. 2 mL of culture medium was added to each well of the blank control group and negative control group, 2 mL of culture medium containing vitamin E was added to each well of the positive control group, and 2 mL of culture medium containing the corresponding concentration of the test sample was added to each well of the sample group. After drug administration, the 6-well plate was incubated in a CO2 incubator (37℃, 5% CO2) for 24 hours.

[0097] 4) Irradiation: According to the test groups, except for the blank control group, all other groups were subjected to UVB irradiation with an irradiation dose of 300 mJ / cm². 2 After irradiation, the samples were placed in a CO2 incubator (37℃, 5% CO2) and cultured for another 24 hours.

[0098] 5) MDA detection: After incubation, cells were collected, and 200 μL of NaOH was added to each tube for lysis. The cells were then placed in a 60°C water bath and cooled. After cooling, 62.5 μL of 35% perchloric acid was added for acidification. The supernatant was collected by centrifugation and thoroughly mixed with 3 μL of 5 mM DNPH solution. The mixture was then incubated at room temperature for 30 min in the dark and detected by HPLC.

[0099] 6) Calculation of MDA downgrade rate The results are shown in Table 4 below.

[0100] Table 4

[0101]

[0102]

[0103] Note: Significance is indicated by *, P-value < 0.05 is indicated by *, P-value < 0.01 is indicated by **.

[0104] Test results show that the composition of the present invention can significantly reduce the content of MDA, and the components have a certain synergistic effect. This indicates that the composition can effectively inhibit lipid peroxidation of the skin, thereby achieving anti-aging effects.

[0105] Experimental Example 3: Stability Test of Pterostilbene

[0106] Solutions from Example 1 and Comparative Examples 1, 2, 4, 7, and 8 were simultaneously subjected to light irradiation (light intensity 4500 Lx ± 500 Lx, 24 hours of continuous light exposure) and stability testing at 45°C for 3 weeks. The residual rate of pterostilbene in the solution was then determined. The residual rate of pterostilbene was calculated using the following formula:

[0107] Pterostilbene residue rate = (actual residue of pterostilbene / initial content of pterostilbene) × 100%

[0108] The method for determining the content of pterostilbene is as follows:

[0109] a. Plotting the Pterostilbene Standard Curve

[0110] Accurately weigh 25.00 mg of pterostilbene standard, place it in a 10 mL volumetric flask, add water and sonicate for 5 min to dissolve, dilute to the mark, and shake well to obtain a linear stock solution (2.00 mg / mL). -1 The linear stock solution was diluted with water to prepare a series of standard solutions with different mass concentration gradients (0.05, 0.1, 0.2, 0.5, 1.0, and 2.0 mg·mL⁻¹). After filtration through a 0.22 μm filter membrane, the solutions were analyzed by HPLC, and the peak areas were recorded. A plot of peak area (Y, mV·s) versus pterostilbene concentration (ρ, mg·mL⁻¹) was plotted. -1 The standard curve of the curve was obtained, the regression coefficient was calculated, and the linear equation was obtained by fitting: Y = 1655217.09ρ + 7438.46, r = 1, which showed good linearity.

[0111] The HPLC detection conditions were as follows: Phenomenex C18 reversed-phase bonded silica gel was used as the stationary phase, with dimensions of 250 mm × 4.6 mm and 5 μm. The mobile phase consisted of acetonitrile and 1% glacial acetic acid in a ratio of 41:59. The flow rate was set to 1.5 mL / min, the detection wavelength was set to 310 nm, and the column temperature was maintained at 40 °C.

[0112] b. Specificity test: The solutions of Comparative Example 3 and Comparative Example 4 were tested in accordance with the above steps. The results showed that the peak positions of the two samples did not overlap, which indicates that the components in Comparative Example 3 did not interfere with the determination of pterostilbene, and the determination method has good specificity.

[0113] c. Determination of Pterostilbene content in samples

[0114] After 3 weeks of investigation, 0.5 mL of the sample solution was accurately transferred, filtered through a 0.22 μm filter membrane, and then analyzed by HPLC. The peak area was recorded, and the actual residue of pterostilbene was calculated according to the standard curve. The residue rate of pterostilbene was also calculated. The results are shown in Table 5 below.

[0115] Table 5. Residual rate of pterostilbene (%) after 3 weeks of sample storage.

[0116] Serial Number name Pterostilbene residue rate under light / % Pterostilbene residue rate at 45℃ / % 1 Example 1 93.74 98.01 2 Comparative Example 1 63.68 70.29 3 Comparative Example 2 69.58 75.87 4 Comparative Example 4 60.28 68.87 5 Comparative Example 7 71.19 80.38 6 Comparative Example 8 94.32 98.32

[0117] As shown in the table above, the residue rate of pterostilbene decreases with increasing time under light and high temperature (45°C), indicating poor stability. However, combining pterostilbene with the probiotic fermented brown algae and 10-hydroxy-2-decenoic acid provided in this invention significantly improves the stability of pterostilbene under light and high temperature (45°C). Furthermore, compared with conventional antioxidants (vitamin E and pentaerythritol tetra(bis-tert-butylhydroxyhydrocinnamate), the composition of this invention demonstrates better stability improvement for pterostilbene than vitamin E and is comparable to bis-tert-butylhydroxyhydrocinnamate.

[0118] Example 4: Evaluation of Anti-wrinkle Effect

[0119] Test sample: Skin lotion from Example 1.

[0120] Subjects: Thirty healthy Chinese women aged 30 to 40 years were selected.

[0121] Test method: Use the sample twice a day, morning and evening, for 4 or 8 weeks.

[0122] The test results were obtained at the following three time periods: before using the sample (D0), 4 weeks after using the sample, and 8 weeks after using the sample.

[0123] Test items: R2, R5 and R7 were measured by the Cutometer (MPA580, Courage and Khazaka, Germany) as parameters for evaluating skin elasticity. The results are shown in Table 6 below.

[0124] Table 6

[0125] Before use After 4 weeks of use After 8 weeks of use R² (mean) 0.512 0.698 0.585 R5 (mean) 0.611 0.698 0.653 R7 (mean) 0.323 0.468 0.403

[0126] Note: The higher the skin elasticity values ​​R2, R5, and R7, the better the skin elasticity.

[0127] The results showed that skin elasticity was significantly improved after using emulsions containing the composition of the present invention, indicating that the composition of the present invention can be used to prepare cosmetics with anti-wrinkle, firming and anti-aging effects.

[0128] The above embodiments are only for illustrating the technical concept and features of the present invention, and are intended to enable those skilled in the art to understand the content of the present invention and implement it accordingly. They should not be construed as limiting the scope of protection of the present invention. All equivalent changes or modifications made in accordance with the spirit and essence of the present invention should be covered within the scope of protection of the present invention.

Claims

1. A composition containing 10-hydroxy-2-decenoic acid, characterized in that, The composition comprises the following components in parts by weight: Probiotic fermented brown algae 0.1-50 parts, pterostilbene 0.001-0.5250 parts, 10-hydroxy-2-decenoic acid 0.001-2 parts; The probiotic-fermented brown algae is a seaweed fermentation broth obtained using the following fermentation method: 1) Soak dried kelp in purified water. Take 1 part kelp and 15 parts water. After soaking the dried kelp thoroughly, rinse it repeatedly with purified water to wash away the mud and salt on the surface of the kelp. 2) Clean kelp is dried at low temperature, and the dried kelp ultrafine powder has a particle size of less than 50μm; 3) Activate the bacterial strains used for fermentation, which are Bacillus amyloliquefaciens, Bacillus licheniformis; Saccharomyces boulardii, Saccharomyces cerevisiae, and Saccharomyces flavoringus; Lactobacillus acidophilus and Lactobacillus exfoliatus; 4) Sterilize the kelp dried at low temperature by irradiation at 1000-2500 krad for 2 hours; 5) The activated Bacillus amyloliquefaciens was cultured in a fermentation medium with the following formula: 1L distilled water + 20g glucose + 15g peptone + 5g sodium chloride + 0.5g beef extract; the Bacillus amyloliquefaciens was cultured until the viable cell count reached 10^6. 9 After reaching cfu / mL, ultra-finely pulverized and sterilized kelp powder was inoculated into the fermentation broth at a ratio of 5%, and Bacillus was cultured further. 6) The kelp powder is degraded by various enzymes produced by the growth of Bacillus, and the kelp and culture medium gradually become a viscous fermented kelp liquid. After 24 hours of fermentation, the fermentation is stopped. 7) After fermentation, centrifuge at 6000 rpm / min for 10 minutes to remove Bacillus cells, and then inoculate 1% Saccharomyces boulardii, 2% Saccharomyces cerevisiae and 2% flavoring yeast respectively according to 5% of the total inoculum for secondary fermentation. The fermentation conditions are 30 degrees Celsius and static culture for 72 hours. 8) After the yeast fermentation is completed, lactic acid bacteria are inoculated at a rate of 5% for a third fermentation. The fermentation conditions are 30 degrees Celsius and static incubation for 48 hours. 9) After the three strains are mixed and fermented, the fermentation broth is placed in a low temperature environment of 10 degrees Celsius for post-ripening transformation, and the post-ripening time is 15 days. 10) After the post-ripening is complete, the liquid is centrifuged and the supernatant is collected. The centrifugation conditions are 10,000 rpm / min for 10 minutes. 11) Filter the supernatant from centrifugation through a 0.22 μm filter membrane. The resulting supernatant is the seaweed fermentation broth. The probiotic-fermented brown algae does not contain live bacteria. The brown algae mentioned is kelp; The 10-hydroxy-2-decenoic acid is an aqueous solution containing 10-hydroxy-2-decenoic acid, and its mass fraction ( w / w The percentage is 1-10%.

2. The composition according to claim 1, characterized in that, The 10-hydroxy-2-decenoic acid is an aqueous solution containing 10-hydroxy-2-decenoic acid, with a mass fraction (w / w) of 5%.

3. The composition according to claim 1, characterized in that, The composition comprises the following components in parts by weight: Five parts of probiotic-fermented brown algae, 0.1 parts of pterostilbene, and 0.1 parts of 10-hydroxy-2-decenoic acid.

4. The use of the composition according to any one of claims 1-3 in the preparation of cosmetics, wherein the cosmetics have at least one or more of the following effects: (a) Anti-wrinkle; (b) Tightening; (c) Anti-aging.

5. A cosmetic product with anti-wrinkle, firming, and anti-aging effects, characterized in that, The cosmetic product comprises at least the composition according to any one of claims 1-3.

6. The cosmetic product as described in claim 5, characterized in that, The cosmetics in question are skincare products.

7. A method for preparing the cosmetic according to any one of claims 5-6, characterized in that, The preparation method includes mixing the composition with other raw material components.

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