Method for improving drought resistance of wheat seeds

Abstract

The invention discloses a method for improving drought resistance of wheat seeds, and belongs to the technical field of wheat drought resistance, and the method comprises the following steps: randomly selecting full, mildew-free and vigorous wheat seeds to be sown, disinfecting for 2 minutes by using 0.1% of raw mercury, and then washing for 5 times by using clear water; soaking the seeds in clear water for 2 hours, filtering moisture of the seeds through a gauze element, and absorbing moisture on the surfaces of the seeds by using filter paper; soaking the seeds in 10% polyethylene glycol 6000 for 3 hours, soaking the seeds in clear water for 2 hours, soaking the seeds in 20% polyethylene glycol 6000 for 4 hours, soaking the seeds in clear water for 2 hours, and soaking the seeds in 30% polyethylene glycol 6000 for 5 hours; after gradual drought stress treatment, the sensitivity of the obtained wheat seeds to water is eliminated, and the adaptive capacity to drought stress is stimulated. According to the method, different water stress environments are set, so that the sensitivity of the seeds to water is eliminated, and the drought resistance of the wheat seeds is improved.

Description

Technical Field

[0001] The invention relates to the technical field of wheat seed drought resistance regulation and control, and in particular to a method for improving wheat seed drought resistance. Background Art

[0002] magnificent

[0003] Wheat is an important food crop in my country and plays a major role in ensuring national food security. In addition, wheat is also a crop that consumes a lot of water. There is insufficient rainfall during the wheat growing period, so rainfall alone cannot meet the growth needs of wheat. Water replenishment irrigation is an important measure to ensure a stable increase in wheat yields.

[0004] Improving the drought resistance of wheat is of great significance to ensuring the safe production of wheat in my country. Wheat-corn rotation cultivation is the main farming method and plays an important role in achieving a double harvest of wheat and corn. However, after harvesting corn, traditional production usually does not carry out supplementary irrigation to create moisture. In order to make timely use of soil moisture, wheat is directly sown. Seed germination is the starting point of the life history of crops and plays a key role in the formation of wheat biomass. The quality of seed germination will directly affect the growth and development of wheat and the establishment of yield. Therefore, improving the drought resistance of wheat seeds during the germination period determines the quality of seedlings after sowing. At present, the existing technical solutions for improving the drought resistance of wheat seeds are mainly "calcium chloride solution treatment method", that is, using 1% concentration of calcium chloride solution to suffocate seeds and 0.1% to 0.2% calcium chloride solution to soak seeds combined with drought-resistant agents to improve the drought resistance of wheat seeds. The prior art "calcium chloride solution treatment method" is based on the action mechanism of calcium chloride in the soil, which can promote the activity of soil microorganisms, improve the structure of the soil, increase the ability of the soil to store water, and reduce the evaporation of water, thereby increasing the ability of crops to absorb water. However, the use of calcium chloride solution treatment method has certain disadvantages: first, it will cause environmental pollution; second, the use concentration and treatment time are relatively strict, and improper use will cause phytotoxicity to wheat; third, calcium chloride is based on soil changes and has a poor drought resistance effect. It has certain limitations in improving the drought resistance of wheat. In order to solve the above problems, the present invention provides a method for regulating the drought resistance of wheat seeds, which eliminates the sensitivity of seeds to water by setting different water stress environments, thereby improving the drought resistance of wheat seeds.

[0005] Aiming at the potential ability of wheat to adapt to survival, in order to overcome the defects of the existing technical solutions, the present invention provides a method for regulating the drought resistance of wheat seeds. Through ingenious drought resistance training, different water stress environments are set up indoors, and different concentrations of polyethylene glycol 6000 are used to gradually eliminate the sensitivity of wheat seeds to water, thereby improving the drought resistance of wheat seeds. This method is safe for the environment, and the drought resistance mechanism is based on wheat itself, so the drought resistance effect is better. Summary of the invention

[0006] In order to solve the above problems, the present invention provides a method for regulating drought-resistant wheat seeds, which cleverly uses drought-resistant training, sets different water stress environments indoors, and uses different concentrations of polyethylene glycol 6000 to gradually eliminate the sensitivity of wheat seeds to water, thereby improving the drought resistance of wheat seeds.

[0007] In order to achieve the above object, the technical solution adopted by the present invention is:

[0008] 1. A method for improving drought resistance of wheat seeds, characterized in that it comprises the following steps:

[0009] S1 randomly selects plump, mildew-free, vigorous wheat seeds to be sown, and disinfects them with 0.1% raw mercury for 2 minutes, then rinses them with clean water 5 times to remove the raw mercury attached to the seed surface;

[0010] S2 Soak the seeds in S1 in clean water for 2 hours, so that the seeds are in contact with the water, but ensure that the water does not submerge the seeds;

[0011] S3 filters the seeds in S2 through a mesh to remove moisture, and uses filter paper to absorb moisture on the surface of the seeds;

[0012] S4: Soak the seeds in S3 in 10% polyethylene glycol 6000 for 3 hours;

[0013] S5: filter the seeds in S4 through a mesh to remove 10% polyethylene glycol 6000;

[0014] S6: Wash the seeds in S5 repeatedly with clean water for 5 times, and then soak them in clean water for 2 hours, so that the seeds are in contact with the water, but ensure that the water does not submerge the seeds;

[0015] S7 filters the seeds in S6 through a mesh to remove moisture, and uses filter paper to absorb moisture on the surface of the seeds;

[0016] S8 Soak the seeds in S7 in 20% polyethylene glycol 6000 for 4 hours;

[0017] S9: filter the seeds in S8 through a mesh to remove 20% polyethylene glycol 6000;

[0018] S10: washing the seeds in S9 with clean water for 5 times, and then soaking them in clean water for 2 hours, so that the seeds are in contact with the water, but the water cannot submerge the seeds;

[0019] S11: filter the seeds in S10 through a mesh to remove moisture, and use filter paper to absorb moisture on the surface of the seeds;

[0020] S12: Soak the seeds in S11 in 30% polyethylene glycol 6000 for 5 hours;

[0021] S13: Filter the seeds in S12 through a mesh to remove 30% polyethylene glycol 6000;

[0022] S14: washing the seeds in S13 repeatedly with clean water for 5 times;

[0023] S15: Use filter paper to absorb the surface moisture of the seeds in S14 and set aside;

[0024] S16 was treated with progressive drought stress, and the wheat seeds in S15 eliminated their sensitivity to water and stimulated their ability to adapt to drought stress.

[0025] 2. A method for improving drought resistance of wheat seeds according to claim 1, characterized in that the mesh used in S3, S5, S7, S9, S11, and S13 should have a mesh size smaller than the thickness of the wheat grains to ensure that the wheat grains are not missed.

[0026] 3. A method for improving drought resistance of wheat seeds according to claim 1, characterized in that the gauze used in S3, S5, S7, S9, S11, and S13 should ensure that the gauze for filtering water and polyethylene glycol 6000 are used separately.

[0027] 4. A method for improving drought resistance of wheat seeds according to claim 3, characterized in that the concentration of polyethylene glycol 6000 in S4, S8 and S12 is mass fraction.

[0028] The present invention has the following beneficial effects:

[0029] This technical solution provides a method for improving the drought resistance of wheat seeds, which solves the problem that the existing technical solutions have certain limitations in improving the drought resistance of wheat. Its disadvantages are: first, it will cause environmental pollution; second, the use concentration and treatment time are relatively strict, and improper use will cause phytotoxicity to wheat; third, calcium chloride is based on soil changes and has a poor drought resistance effect. This technical solution cleverly uses drought resistance training, sets up different water stress environments indoors, and uses different concentrations of polyethylene glycol 6000 to gradually eliminate the sensitivity of wheat seeds to water, thereby improving the drought resistance of wheat seeds, which can effectively solve the disadvantages of the existing technical solutions. DETAILED DESCRIPTION

[0030] In order to make the purpose and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0031] Example:

[0032] 1. A method for improving drought resistance of wheat seeds, characterized in that it comprises the following steps:

[0033] S1 randomly selects plump, mildew-free, vigorous wheat seeds Hansheng 730 to be sown, and disinfects the Hansheng 730 seeds with 0.1% raw mercury for 2 minutes, then rinses the Hansheng 730 seeds with clean water for 5 times, and removes the raw mercury attached to the surface of the seeds;

[0034] S2: Soak the sterilized Hansheng 730 seeds in clean water for 2 hours, ensuring that the seeds are in contact with the water but the water cannot submerge the seeds;

[0035] S3: filtering the water from the Hansheng 730 seeds in S2 through a mesh with mesh holes smaller than the coarseness of the Hansheng 730 grains and only used for filtering water, and absorbing the water on the surface of the Hansheng 730 seeds with filter paper;

[0036] S4: Soak the seeds of Hansheng 730 in S3 in 10% polyethylene glycol 6000 for 3 hours;

[0037] S5: filtering the Hansheng 730 seeds in S4 through a gauze having meshes smaller than the coarseness of Hansheng 730 grains and only used for filtering polyethylene glycol 6000 to remove 10% of polyethylene glycol 6000;

[0038] S6: repeatedly washing the Hansheng 730 seeds in S5 with clean water for 5 times, and then soaking them in clean water for 2 hours, ensuring that the Hansheng 730 seeds are in contact with water, but the water cannot submerge the Hansheng 730 seeds;

[0039] S7: filtering the Hansheng 730 seeds in S6 through a mesh having mesh holes smaller than the coarseness of Hansheng 730 grains and used only for filtering water, and absorbing the moisture on the surface of the Hansheng 730 seeds with filter paper;

[0040] S8: soaking the seeds of Hansheng 730 in S7 in 20% polyethylene glycol 6000 for 4 hours;

[0041] S9: filtering the Hansheng 730 seeds in S8 through a gauze having mesh holes smaller than the coarseness of Hansheng 730 grains and only used for filtering polyethylene glycol 6000 to remove 20% of polyethylene glycol 6000;

[0042] S10: repeatedly washing the Hansheng 730 seeds in S9 with clean water for 5 times, and then soaking them in clean water for 2 hours, ensuring that the Hansheng 730 seeds are in contact with water, but the water cannot submerge the Hansheng 730 seeds;

[0043] S11: filtering the water from the Hansheng 730 seeds in S10 through a mesh with mesh holes smaller than the coarseness of the Hansheng 730 grains and only used for filtering water, and absorbing the water on the surface of the Hansheng 730 seeds with filter paper;

[0044] S12: soaking the seeds of Hansheng 730 in S11 in 30% polyethylene glycol 6000 for 5 hours;

[0045] S13: filtering the Hansheng 730 seeds in S12 through a gauze having mesh holes smaller than the coarseness of Hansheng 730 grains and only used for filtering polyethylene glycol 6000 to remove 30% of the polyethylene glycol 6000;

[0046] S14: Wash the seeds of Hansheng 730 in S13 repeatedly with clean water for 5 times;

[0047] S15: Use filter paper to absorb the surface moisture of the seeds of Hansheng 730 in S14 and set aside;

[0048] S16 was treated with progressive drought stress, and the Hansheng 730 wheat seeds in S15 eliminated their sensitivity to water and stimulated their ability to adapt to drought stress (the drought resistance data of Hansheng 730 treated under drought stress are shown in Table 1).

[0049] As can be seen from Table 1, under the conditions of 25% polyethylene glycol 6000 indoor simulated drought stress, the root length, seedling height and lateral root number of Hansheng 730 seeds treated with progressive drought stress were higher than those of Hansheng 730 seeds not treated with progressive drought stress, indicating that the Hansheng 730 seeds treated with progressive drought stress eliminated their sensitivity to water, thereby improving the drought resistance of Hansheng 730 wheat seeds.

[0050] Table.1 Analysis of drought resistance of Hansheng 730 treated under drought stress

[0051]

[0052] Note: A is the seeds of Hansheng 730 treated with progressive drought stress; B is the seeds of Hansheng 730 not treated with progressive drought stress; A and B were cultured indoors for 5 days under 25% polyethylene glycol 6000 stress, and the root length and seedling height were measured with a ruler, and the number of lateral roots was investigated.

[0053] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements or modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A method for improving drought resistance of wheat seeds, characterized in that: The following steps are involved: S1 randomly selects plump, mildew-free, vigorous wheat seeds to be sown, and disinfects them with 0.1% raw mercury for 2 minutes, then rinses them with clean water for 5 times to remove the raw mercury attached to the surface of the seeds; S2 Soak the seeds in S1 in clean water for 2 hours, so that the seeds are in contact with the water, but ensure that the water does not submerge the seeds; S3 filters the seeds in S2 through a mesh to remove moisture, and uses filter paper to absorb moisture on the surface of the seeds; S4: Soak the seeds in S3 in 10% polyethylene glycol 6000 for 3 hours; S5: filter the seeds in S4 through a mesh to remove 10% polyethylene glycol 6000; S6: Wash the seeds in S5 repeatedly with clean water for 5 times, and then soak them in clean water for 2 hours, so that the seeds are in contact with the water, but ensure that the water does not submerge the seeds; S7 filters the seeds in S6 through a mesh to remove moisture, and uses filter paper to absorb moisture on the surface of the seeds; S8 Soak the seeds in S7 in 20% polyethylene glycol 6000 for 4 hours; S9: filter the seeds in S8 through a mesh to remove 20% polyethylene glycol 6000; S10: washing the seeds in S9 with clean water for 5 times, and then soaking them in clean water for 2 hours, so that the seeds are in contact with the water, but the water cannot submerge the seeds; S11: filter the seeds in S10 through a mesh to remove moisture, and use filter paper to absorb moisture on the surface of the seeds; S12 Soak the seeds in S11 in 30% polyethylene glycol 6000 for 5 hours; S13: Filter the seeds in S12 through a mesh to remove 30% polyethylene glycol 6000; S14: washing the seeds in S13 repeatedly with clean water for 5 times; S15: Use filter paper to absorb the surface moisture of the seeds in S14 and set aside; S16 was treated with progressive drought stress, and the wheat seeds in S15 eliminated their sensitivity to water and stimulated their ability to adapt to drought stress.

2. A method for improving drought resistance of wheat seeds according to claim 1, characterized in that: The meshes used in S3, S5, S7, S9, S11, and S13 should have mesh openings smaller than the size of the wheat grains to ensure that the wheat grains are not missed.

3. A method for improving drought resistance of wheat seeds according to claim 1, characterized in that: The gauze used in S3, S5, S7, S9, S11, and S13 should ensure that the gauze for filtering water and polyethylene glycol 6000 are used separately.

4. A method for improving drought resistance of wheat seeds according to claim 3, characterized in that: The concentration of polyethylene glycol 6000 described in S4, S8 and S12 is a mass fraction.

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