Eye-protecting nutrition powder and preparation method thereof

Abstract

The invention belongs to the technical field of health care, and particularly relates to eye protection nutrition powder and a preparation method thereof, the eye protection nutrition powder comprises glucose, whole milk powder, fruit powder, marigold powder, flaxseed powder, seaweed meal, haematococcus pluvialis powder, lutein ester, zeaxanthin, beta-carotene, ceramide and DHA algae oil powder. The eye protection nutrition powder is good in absorption effect, improves visual fatigue and yellow bottom spot lesion, and effectively protects eyes; and the concentration and memory can be improved. According to the eye-protecting nutrition powder prepared by adopting a biological wall-breaking and multi-flora fermentation technology, effective components can be dissolved out to the maximum extent after the eye-protecting nutrition powder is taken, and the nutrition powder prepared by the method is easy to absorb by a human body and high in bioavailability.

Description

Technical Field

[0001] The invention belongs to the technical field of health care, and in particular relates to an eye protection nutrient powder and a preparation method thereof. Background Art

[0002] With the advancement of science and technology, long-term use of electronic products, various radiation and strong light pollution increase the consumption of lutein in the eyes, and changes in living habits, such as the reduction of vegetables and other vegetarian foods, reduce the supplement of lutein in the eyes, so the number of people with visual fatigue and myopia increases sharply. The eyes need to gather a large amount of lutein to absorb harmful strong light such as ultraviolet rays and blue light to protect the health of the retina; therefore, the eyes consume a large amount of lutein every day, and lutein cannot be synthesized in the body and must be supplemented through external intake.

[0003] For the above reasons, health products using blueberry or lutein as raw materials are increasing day by day, for example: Patent CN103734712B is a blueberry and raspberry functional food for eye protection, which discloses food compositions for protecting vision, relieving eye fatigue and preventing eye damage, blueberry polyphenol extract, raspberry polyphenol extract, wolfberry extract, zeaxanthin extract and marigold lutein extract, etc. Patent CN112384232B discloses a pharmaceutical composition and health functional food for preventing or treating macular degeneration, including Banaba leaf extract and guava leaf extract; it can effectively prevent or treat macular degeneration, inhibit the photo-oxidation of A2E caused by blue light, etc. Patent CN110403200A is a kudzu rice composition and its application in relieving eye fatigue and improving macular lesions. The patent discloses that kudzu rice is used as the main raw material, and different formula combinations and different preparation methods are used to obtain products with the functions of relieving eye fatigue and improving cognitive / memory ability.

[0004] In the prior art, extracts of various substances are extracted by a single mechanical method or solvent immersion method, but the extraction efficiency is limited, and the developed products have limited absorption and utilization in the body, which is insufficient. Summary of the invention

[0005] In order to solve the deficiencies of the prior art, the purpose of the present invention is to provide an eye protection nutritional powder and a preparation method thereof, and to develop a new preparation method - using biological wall breaking and multi-bacteria fermentation technology to improve the extraction efficiency of each component, which is conducive to improving the absorption rate of the effective ingredients of the prepared nutritional components in the body; the nutritional powder of the present invention has a good absorption effect, and can maximize the dissolution of the effective ingredients after taking it, improve visual fatigue, yellow spot lesions, and effectively protect the eyes; it can also improve concentration and memory. The nutritional powder prepared by the method of the present invention is easily absorbed by the human body and has a high bioavailability.

[0006] To achieve the above object, the technical solution of the present invention is as follows:

[0007] On the one hand, the present invention provides an eye protection nutritional powder, comprising glucose, whole milk powder, fruit powder, marigold powder, flaxseed powder, seaweed powder, Haematococcus pluvialis powder, lutein ester, zeaxanthin, β-carotene, ceramide and DHA algae oil powder.

[0008] Further, the fruit powder comprises at least one of acai berry powder, blueberry fruit powder, cranberry fruit powder, black wolfberry fruit powder and red grape skin powder. Further, the fruit powder comprises a mixture of acai berry powder, blueberry fruit powder, cranberry fruit powder, black wolfberry fruit powder and red grape skin powder.

[0009] Furthermore, the nutritional powder includes, by mass, 15 to 40 parts of glucose, 15 to 40 parts of whole milk powder, 2 to 10 parts of acai berry powder, 2 to 10 parts of blueberry powder, 2 to 10 parts of marigold powder, 0.5 to 5 parts of flaxseed powder, 0.5 to 5 parts of cranberry powder, 1 to 5 parts of black wolfberry powder, 0.5 to 5 parts of seaweed powder, 0.1 to 1 parts of red grape skin powder, 0.1 to 1 parts of Haematococcus pluvialis powder, 0.5 to 3 parts of lutein ester, 0.1 to 1 parts of zeaxanthin, 0.1 to 1 parts of β-carotene, 0.05 to 1 parts of ceramide and 0.5 to 5 parts of DHA algae oil powder.

[0010] Preferably, the nutritional powder comprises, by mass, 30 parts of glucose, 30 parts of whole milk powder, 5 parts of acai berry powder, 5 parts of blueberry fruit powder, 5 parts of marigold powder, 1 part of flaxseed powder, 1 part of cranberry fruit powder, 2 parts of black wolfberry fruit powder, 1 part of seaweed powder, 0.5 parts of red grape skin powder, 0.5 parts of Haematococcus pluvialis powder, 1 part of lutein ester, 0.4 parts of zeaxanthin, 0.5 parts of β-carotene, 0.1 parts of ceramide and 1 part of DHA algae oil powder.

[0011] Preferably, the content of the lutein ester is 5%; the content of the zeaxanthin is 5%.

[0012] Ceramide also plays an important structural role in cell membranes, increasing the rigidity of cell membranes, creating microdomains, and changing membrane permeability. Ceramide can also enhance the bioavailability of anthocyanins, lutein, etc. in nutritional powders by acting as a lipid delivery system. Ceramide can relieve eye diseases.

[0013] On the other hand, the present invention also provides a method for preparing an eye-protecting nutritional powder, comprising the following steps:

[0014] S1: sifting and mixing fruit powder, marigold powder, flaxseed powder, seaweed powder, Haematococcus pluvialis powder and DHA algae oil powder, adding water, adding acid solution to adjust pH, and standing to obtain a mixed solution;

[0015] S2: pasteurize the mixed solution, add bacterial suspension after cooling, and ferment to obtain biological wall-breaking eye protection agent;

[0016] S3: adding an alkaline solution to the biological wall-breaking eye protection main agent, adjusting the pH to 6.5-7.3, filtering, concentrating and drying the filtrate to obtain a preparatory nutrient powder;

[0017] S4: Mixing glucose, whole milk powder, lutein ester, zeaxanthin, β-carotene and ceramide with the prepared nutritional powder to prepare nutritional powder.

[0018] Furthermore, in step S1, the solid-liquid mass ratio of the total mass of the fruit powder, marigold powder, flaxseed powder, seaweed powder, Haematococcus pluvialis powder and DHA algae oil powder to water is 1:(10-50), preferably 1:10, 1:20, 1:30, 1:40 or 1:50.

[0019] Furthermore, the pH is adjusted to 3.2-3.4, and the standing time is 10-60 min. Preferably, the standing time is 10, 20, 30, 40, 50 or 60 min.

[0020] Furthermore, in step S1, the acid solution is a citric acid solution with a mass fraction of 0.1-0.5%, preferably 0.1%, 0.2%, 0.3%, 0.4% or 0.5%.

[0021] Furthermore, in step S2, the pasteurization treatment condition is 70-90°C for 10-30 min, preferably 70, 80 or 90°C for 10, 20 or 30 min.

[0022] Furthermore, in step S2, the mass ratio of the mixed solution to the bacterial suspension is 100:(2.5-5), preferably 100:2.5, 100:4, 100:5, etc.

[0023] Furthermore, in step S2, the bacterial suspension includes lactic acid bacteria and yeast, and the concentration of lactic acid bacteria is 5×10 6 ~8×10 6 cfu / ml, the concentration of yeast is 1×10 6 ~2×10 6 cfu / ml. Removing other bacteria after pasteurization is conducive to the subsequent addition of beneficial bacteria - bacterial suspension, providing a beneficial environment for its fermentation.

[0024] Furthermore, in step S2, the fermentation time is 3 to 4 hours.

[0025] Further, in step S2, cooling is performed to 32°C±2°C.

[0026] In the present invention, an acid solution is first used to destroy the cell wall structure of raw materials such as fruit powder, and then lactic acid bacteria and yeast are selected to ferment together with a suitable fermentation process to ferment the fruit powder in step S1, thereby further destroying the cell wall and releasing substances in the cell, such as anthocyanins, lutein and α-linolenic acid.

[0027] Furthermore, in step S3, the alkaline solution is a sodium hydroxide solution with a mass fraction of 0.5-4.0% (preferably 0.5%, 1%, 1.5%, 2%, 3% or 4%), and a pH value of 6.5-7.3, preferably 6.8. The pH value is the best value for human absorption.

[0028] In another aspect, the present invention provides use of the nutritional powder of the present invention in preparing products (such as food or health products) for protecting eyes, improving macular degeneration, and improving concentration and memory.

[0029] More specifically, the food includes but is not limited to: fermented dairy products, cheese, milk-containing beverages, solid beverages, compressed candies, milk tablets, and milk powder.

[0030] More specifically, the health care products include but are not limited to: liquid health care products, granular health care products, powdered health care products, capsule health care products or tablet health care products.

[0031] Compared with the prior art, the beneficial technical effects of the present invention are as follows:

[0032] Compared with the prior art, the eye protection nutritional powder provided by the present invention is rich in anthocyanins, lutein, α-linolenic acid and other eye protection ingredients through scientific proportioning of raw materials, and has the functions of improving visual fatigue, yellow spot lesions, and improving concentration and memory.

[0033] The present invention uses an acid solution to first destroy the cell wall structure of raw materials such as fruit powder, and then selects lactic acid bacteria and yeast for combined fermentation and cooperates with a suitable fermentation process to ferment the fruit powder in step S1, further destroying the cell wall to maximize the dissolution of the effective ingredients. The nutrient powder prepared by the method is easily absorbed by the human body and has a high bioavailability. DETAILED DESCRIPTION

[0034] The technical solution of the present invention is illustrated below by way of example, and the scope of protection requested by the present invention includes but is not limited to the following embodiments.

[0035] Example 1

[0036] An eye protection nutrient powder comprises 30 parts of glucose, 30 parts of whole milk powder, 5 parts of acai berry powder, 5 parts of blueberry fruit powder, 5 parts of marigold powder, 1 part of flaxseed powder, 1 part of cranberry fruit powder, 2 parts of black wolfberry fruit powder, 1 part of seaweed powder, 0.5 parts of red grape skin powder, 0.5 parts of Haematococcus pluvialis powder, 1 part of lutein ester (content is 5%), 0.4 parts of zeaxanthin (content is 5%), 0.5 parts of beta-carotene, 0.1 parts of ceramide and 1 part of DHA algae oil powder.

[0037] A method for preparing eye protection nutritional powder comprises the following steps:

[0038] S1: sieving and mixing fruit powder, marigold powder, flax seed powder, seaweed powder, Haematococcus pluvialis powder and DHA algae oil powder to obtain a mixture, adding 30 times the mass of water of the mixture, adding 0.1% citric acid solution to adjust the pH to 3.4, and standing for 10 minutes to obtain a mixed solution;

[0039] S2: pasteurize the mixed solution at 80℃ for 15min, cool it to 32℃±2℃, add the bacterial suspension, and ferment it for 4h to obtain the biological wall-breaking eye protection agent;

[0040] S3: adding a sodium hydroxide solution with a mass fraction of 1.0% to the biological wall-breaking eye protection main agent, adjusting the pH to 6.5, filtering, and concentrating and drying the filtrate to obtain a preparatory nutrient powder;

[0041] S4: Mixing glucose, whole milk powder, lutein ester (content of 5%), zeaxanthin (content of 5%), β-carotene and ceramide with the prepared nutritional powder to prepare nutritional powder.

[0042] In step S2, the mass ratio of the mixed solution to the bacterial suspension is 100:3; the concentration of lactic acid bacteria in the bacterial suspension is 6×10 6 cfu / ml, the concentration of yeast is 1×10 6 cfu / ml.

[0043] Example 2

[0044] An eye protection nutrient powder comprises 40 parts of glucose, 20 parts of whole milk powder, 8 parts of acai berry powder, 2 parts of blueberry fruit powder, 10 parts of marigold powder, 5 parts of flaxseed powder, 0.5 parts of cranberry fruit powder, 5 parts of black wolfberry fruit powder, 5 parts of seaweed powder, 1 part of red grape skin powder, 1 part of Haematococcus pluvialis powder, 3 parts of lutein ester (content is 5%), 1 part of zeaxanthin (content is 5%), 1 part of beta-carotene, 1 part of ceramide and 3 parts of DHA algae oil powder.

[0045] A method for preparing eye protection nutritional powder comprises the following steps:

[0046] S1: sieving and mixing fruit powder, marigold powder, flax seed powder, seaweed powder, Haematococcus pluvialis powder and DHA algae oil powder to obtain a mixture, adding 25 times the mass of water of the mixture, adding 0.5% by mass citric acid solution to adjust the pH to 3.2, and standing for 20 minutes to obtain a mixed solution;

[0047] S2: pasteurize the mixed solution at 90℃ for 10min, cool it to 32℃±2℃, add the bacterial suspension, and ferment it for 3h to obtain the biological wall-breaking eye protection agent;

[0048] S3: adding a sodium hydroxide solution with a mass fraction of 1.5% to the biological wall-breaking eye protection main agent, adjusting the pH to 6.8, filtering, and concentrating and drying the filtrate to obtain a preparatory nutrient powder;

[0049] S4: Mixing glucose, whole milk powder, lutein ester (content of 5%), zeaxanthin (content of 5%), β-carotene and ceramide with the prepared nutritional powder to prepare nutritional powder.

[0050] In step S2, the mass ratio of the mixed solution to the bacterial suspension is 100:3; the concentration of lactic acid bacteria in the bacterial suspension is 8×10 6 cfu / ml, the concentration of yeast was 2×10 6 cfu / ml.

[0051] Example 3

[0052] An eye protection nutrient powder comprises 15 parts of glucose, 40 parts of whole milk powder, 2 parts of acai berry powder, 8 parts of blueberry fruit powder, 8 parts of marigold powder, 4 parts of flaxseed powder, 3 parts of cranberry fruit powder, 3 parts of black wolfberry fruit powder, 3 parts of seaweed powder, 0.8 parts of red grape skin powder, 0.8 parts of Haematococcus pluvialis powder, 3 parts of lutein ester (content is 5%), 0.7 parts of zeaxanthin (content is 5%), 0.9 parts of beta-carotene, 0.2 parts of ceramide and 3 parts of DHA algae oil powder.

[0053] A method for preparing eye protection nutritional powder comprises the following steps:

[0054] S1: sieving and mixing fruit powder, marigold powder, flax seed powder, seaweed powder, Haematococcus pluvialis powder and DHA algae oil powder to obtain a mixture, adding 15 times the mass of water of the mixture, adding 0.1% citric acid solution to adjust the pH to 3.4, and standing for 20 minutes to obtain a mixed solution;

[0055] S2: pasteurize the mixed solution at 80℃ for 15min, cool it to 32℃±2℃, add the bacterial suspension, and ferment it for 4h to obtain the biological wall-breaking eye protection agent;

[0056] S3: adding a sodium hydroxide solution with a mass fraction of 1.0% to the biological wall-breaking eye protection main agent, adjusting the pH to 6.5, filtering, and concentrating and drying the filtrate to obtain a preparatory nutrient powder;

[0057] S4: Mixing glucose, whole milk powder, lutein ester (content of 5%), zeaxanthin (content of 5%), β-carotene and ceramide with the prepared nutritional powder to prepare nutritional powder.

[0058] In step S2, the mass ratio of the mixed solution to the bacterial suspension is 100:3; the concentration of lactic acid bacteria in the bacterial suspension is 6×10 6 cfu / ml, the concentration of yeast is 1×10 6 cfu / ml.

[0059] Comparative Example 1

[0060] The difference from Example 1 is that in S1, the citric acid solution is replaced by water.

[0061] Comparative Example 2

[0062] The difference from Example 1 is that in S2, no fermentation process is performed.

[0063] Comparative Example 3

[0064] The difference from Example 1 is that the bacterial suspension contains only lactic acid bacteria at a concentration of 6×10 6 cfu / ml.

[0065] Comparative Example 4

[0066] The difference from Example 1 is that the bacterial suspension contains only yeast at a concentration of 1×10 6 cfu / ml.

[0067] Effect example

[0068] (1) Experimental methods

[0069] Balb-c mice (5 weeks old, male) were adapted to the environment and raised for 2 days, and the treatment group was orally administered with the nutrient powder prepared in Example 1 and Comparative Examples 1-4 once a day for 7 days. Then, after 24 hours of dark adaptation, the nutrient powder prepared in Example 1 and Comparative Examples 1-4 was orally administered for 14 days, and 30 minutes after administration every day, blue light was irradiated at 10,000 lux for 1 hour. 24 hours after the end of blue light irradiation and nutrient powder administration, the mice were killed to remove the eyeballs.

[0070] A blank group and a control group were set up. Neither the blank group nor the control group was given nutrient powder. The blank group was not irradiated with blue light, while the control group was irradiated with blue light.

[0071] The removed eyeball tissue is fixed with a neutral buffered formalin solution. The fixed eyeball is washed with running water and dehydrated with alcohol. Thereafter, xylene is used for transparency treatment, and paraffin is used to give the tissue appropriate rigidity. The eyeball is made into a paraffin block of a predetermined shape and then cut into a thickness of 4 μm. The cut eyeball is stained with hematoxylin and eosin (H&E). After preferential treatment with hematoxylin, the eyeball is placed at room temperature for 30 seconds and then washed with running water for 10 minutes. Afterwards, the eyeball is treated with eosin for 1 minute. The stained organ is observed using a microscope at a distance of 600 to 900 μm from the optic nerve. Then, the thickness of the outer nuclear layer (ONL) of the visual cell is measured by Image J software, and the number of nuclei is counted.

[0072] (2) Experimental results

[0073] The ONL thickness of the control group irradiated with blue light decreased by 45.7% compared with the blank group that was not irradiated with blue light.

[0074] The difference in ONL thickness between the blank group not irradiated with blue light and the blue light irradiated control group was set as 100%.

[0075] The ONL thickness protective effect caused by blue light in each administration group was estimated by calculating the ONL protective effect by the difference in ONL thickness between the blue light irradiation control group and the nutrient powder-treated group. The results of measuring the ONL thickness of the photoreceptor cells are shown in Table 1.

[0076] Table 1

[0077]

[0078]

[0079] It can be seen from the test results in Table 1 that in the blue light irradiation-induced macular degeneration model, the nutrient powder prepared by the preparation method of the present invention has a better effect on repairing retinal cell damage than the nutrient powder prepared in Comparative Examples 1 to 4.

[0080] It can be seen from the repair effects of Comparative Example 1 and Example 1 that biological wall-breaking treatment of some substances in the nutrient powder by acid greatly facilitates the subsequent fermentation of multiple bacterial flora and dissolution of effective ingredients.

[0081] It can be seen from the repair effects of Comparative Example 2 and Example 1 that without bacterial fermentation, some substances in the nutrient powder cannot be effectively dissolved, resulting in a relative lack of components that are beneficial to human absorption, and thus a poor repair effect.

[0082] It can be seen from the repair effects of Comparative Examples 3 to 4 and Example 1 that the dissolution effect of nutrient-effective substances is best through fermentation of multiple bacterial communities, which can effectively improve the repair effect.

[0083] Example 1 of the present application adopts biological cell wall breaking combined with fermentation of multiple bacterial flora to maximize the dissolution of effective ingredients. The nutrient powder prepared by this method is easily absorbed by the human body and has high bioavailability. The eye protection nutrient powder prepared in this application has the functions of protecting eyes, improving fundus macular degeneration, improving concentration and memory. Starting from the improvement of fundus macular degeneration in Table 1, it can further improve the improvement of fundus macular degeneration.

[0084] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. An eye protection nutritional powder, characterized in that: Including glucose, whole milk powder, fruit powder, marigold powder, flaxseed powder, seaweed powder, Haematococcus pluvialis powder, lutein ester, zeaxanthin, beta-carotene, ceramide and DHA algae oil powder; Furthermore, the fruit powder includes at least one of acai berry powder, blueberry fruit powder, cranberry fruit powder, black wolfberry fruit powder and red grape skin powder.

2. The nutritional powder according to claim 1, characterized in that Calculated by mass, it includes: 15-40 parts of glucose, 15-40 parts of whole milk powder, 2-10 parts of acai berry powder, 2-10 parts of blueberry fruit powder, 2-10 parts of marigold powder, 0.5-5 parts of flaxseed powder, 0.5-5 parts of cranberry fruit powder, 1-5 parts of black wolfberry fruit powder, 0.5-5 parts of seaweed powder, 0.1-1 parts of red grape skin powder, 0.1-1 parts of Haematococcus pluvialis powder, 0.5-3 parts of lutein ester, 0.1-1 parts of zeaxanthin, 0.1-1 parts of beta-carotene, 0.05-1 parts of ceramide and 0.5-5 parts of DHA algae oil powder.

3. The nutritional powder according to claim 1, characterized in that Calculated by mass, it includes: 30 parts of glucose, 30 parts of whole milk powder, 5 parts of acai berry powder, 5 parts of blueberry fruit powder, 5 parts of marigold powder, 1 part of flaxseed powder, 1 part of cranberry fruit powder, 2 parts of black wolfberry fruit powder, 1 part of seaweed powder, 0.5 parts of red grape skin powder, 0.5 parts of Haematococcus pluvialis powder, 1 part of lutein ester, 0.4 parts of zeaxanthin, 0.5 parts of beta-carotene, 0.1 parts of ceramide and 1 part of DHA algae oil powder.

4. The nutritional powder according to claim 1, characterized in that The content of the lutein ester is 5%; the content of the zeaxanthin is 5%.

5. A method for preparing the nutritional powder according to any one of claims 1 to 4, characterized in that: The steps include: S1: sifting and mixing fruit powder, marigold powder, flaxseed powder, seaweed powder, Haematococcus pluvialis powder and DHA algae oil powder, adding water, adding acid solution to adjust pH, and standing to obtain a mixed solution; S2: pasteurize the mixed solution, add bacterial suspension after cooling, and ferment to obtain biological wall-breaking eye protection agent; S3: adding an alkaline solution to the biological wall-breaking eye protection main agent, adjusting the pH, filtering, and concentrating and drying the filtrate to obtain a preparatory nutrient powder; S4: Mixing glucose, whole milk powder, lutein ester, zeaxanthin, β-carotene and ceramide with the prepared nutritional powder to prepare nutritional powder.

6. The preparation method according to claim 5, characterized in that: In step S1, the solid-liquid mass ratio of the total mass of fruit powder, marigold powder, flaxseed powder, seaweed powder, Haematococcus pluvialis powder and DHA algae oil powder to water is 1:(10-50); the acid solution is a citric acid solution with a mass fraction of 0.1-0.5%; further, the pH is 3.2-3.4, and the standing time is 10-60 minutes.

7. The preparation method according to claim 5, characterized in that: In step S2, the pasteurization treatment conditions are 70-90°C and the time is 10-30 min; the mass ratio of the mixed solution to the bacterial suspension is 100:(2.5-5); the bacterial suspension includes a suspension of lactic acid bacteria and yeast; the fermentation time is 3-4 h; and the mixture is cooled to 32°C±2°C.

8. The preparation method according to claim 7, characterized in that: In the bacterial suspension, the concentration of lactic acid bacteria is 5×10 6 ~8×10 6 cfu / ml, the concentration of yeast is 1×10 6 ~2×10 6 cfu / ml.

9. The preparation method according to claim 5, characterized in that: In step S3, the alkaline solution is a sodium hydroxide solution with a mass fraction of 0.5-4.0%; the pH value is 6.5-7.

3.

10. Use of the nutritional powder according to any one of claims 1 to 4 or the nutritional powder prepared by the preparation method according to any one of claims 5 to 9 in preparing food or health products for protecting eyes, improving macular degeneration, and improving concentration and memory.

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