A prestressed vascular chip

Abstract

The present invention discloses a prestressed vascular chip, which relates to the field of medical vascular technology. The device includes a main body, a first chamber and a third chamber are opened on the surface of the main body, the first chamber is connected to the third chamber through a first channel, and the main body is provided with at least two fourth chambers, the fourth chamber is arranged on one side of the first chamber, and there is a connecting channel between the fourth chambers, and at least two first channels connected to the first chamber are provided on the connecting channel. The vascular chip that can be constructed by the present invention can achieve a mechanical performance morphology similar to the mechanical properties of blood vessels in the body, so as to solve the problem that the mechanical performance state of the vascular channel inside the chip of the prior art is quite different from the actual vascular state of the human body.

Description

Technical Field

[0001] The present invention relates to the technical field of medical blood vessels, and in particular to a prestressed blood vessel chip. Background Art

[0002] Vascular chips are a typical type of microfluidic chip. In addition to its miniaturization, integration, and low power consumption, vascular chip technology can also precisely control multiple system parameters, such as chemical concentration gradients, fluid shear force, as well as the construction of cell pattern culture and tissue-tissue interface interactions, to simulate the complex structure, microenvironment, and physiological functions of human blood vessels. Currently, interventional therapy is the most important treatment for cardiovascular disease, and the implantation of vascular stents is the most commonly used method in interventional therapy. The development of new vascular stents that are more suitable for the complex internal environment of the human body is of great significance to alleviating the increasingly severe incidence of cardiovascular diseases.

[0003] Currently, relevant evaluation and research experiments are typically limited to testing in animal models. Animal experiments are subject to numerous limitations, including the need to control the animal's physiological state and surgical conditions, the cost of animal husbandry, and the cost of establishing animal models. Furthermore, there are drawbacks such as long preparation times and complex procedures. PDMS (polydimethylsiloxane) is currently one of the most common materials for microfluidic chips. PDMS offers excellent flexibility, transparency, and biocompatibility, as well as ease of processing and fabrication of microfluidic structures. Therefore, it is widely used in microfluidic chip research and applications in biomedicine and chemical analysis. PDMS microfluidic chips enable precise control and manipulation of microfluidics, and offer advantages such as high throughput, high sensitivity, and high integration. Consequently, they have found widespread application in bioanalysis, cell culture, and drug screening. Vascular chip technology is rapidly developing, with a variety of approaches emerging, including fluid-stimulated vascularized organoid chips and 3D-printed tubing structures using coiled rods.

[0004] In order to solve the above problems, the prior art provides many solutions. For example, the prior art US18678112 discloses a microfluidic device, which includes a microfluidic chip for producing micro organic spheres (MOS). The first channel is defined in the surface of the microfluidic chip and includes: a droplet generating part, the droplet generating part includes an inlet part, a junction between the inlet part and the emulsification fluid channel, and a chamber downstream of the junction. The cross-sectional area of ​​the chamber is larger than the cross-sectional area of ​​the inlet part. The first channel includes a polymerization part downstream of the droplet generating part, and the polymerization part has a serpentine structure. The device includes a barrel for MOS demulsification, and the barrel includes: a collection container; a substrate arranged on the collection container, and a membrane arranged between the collection container and the surface of the substrate. The second channel is defined in the surface of the substrate facing the collection container and is connected to the output fluid of the polymerization part of the first channel, but the device still has room for improvement in controlling the inflow of the medium. Summary of the Invention

[0005] The purpose of the present invention is to provide a prestressed vascular chip. The constructed vascular chip can achieve a mechanical performance morphology similar to the mechanical properties of blood vessels in the body, so as to solve the problem that the mechanical performance state of the vascular channels inside the chip of the existing technology is quite different from the actual vascular state of the human body.

[0006] To solve the above technical problems, the present invention specifically provides the following technical solutions: a prestressed vascular chip, comprising a body, a first chamber and a third chamber defined on a surface of the body, the first chamber communicating with the third chamber via a first channel, and at least two fourth chambers disposed on the body, the fourth chambers being located on one side of the first chamber and having a connecting channel between the fourth chambers, wherein the connecting channel is provided with at least two first channels communicating with the first chamber. The present invention provides a first chamber for cell culture or testing, a first channel for simulating a blood vessel, and at least two fourth chambers to create a structure in which, when a culture fluid or medium is input, the medium passes through the fourth chamber and generates different output pressures to the first and third chambers after passing through the fourth chambers. The medium flows through the connecting channel at different rates into each fourth chamber, resulting in different pressures and flow rates of the medium flowing out of these fourth chambers. This results in different pressures in the first channel and subsequent flow paths, simulating the different responses of a blood vessel to different media and flow rates, and ensuring that the medium input closely resembles the state of a real blood vessel.

[0007] At the same time, the above design can provide a variety of liquid inlet schemes, and it is also possible to choose to retain only a single fourth chamber and a single connecting channel. The specific data can be freely selected according to the experimental purpose, and the experimental application range is relatively wide.

[0008] According to one embodiment of the present invention, the main body includes an extension plate, to which the first tube is connected, creating a communication channel between the first tube and the fourth chamber. The first tube is a capillary tube used to supply medium to the fourth chamber. The extension plate stabilizes the input at the interface between the first tube and the fourth chamber, resolving the issue of inflow flow rate, flow rate, and pressure being inconsistent with the pump input flow rate, flow rate, and pressure due to a bend in the first tube and the fourth chamber.

[0009] According to one embodiment of the present invention, the fourth chamber is a circular chamber, and protrusions with a spacing are arranged in an array around the fourth chamber, and the side of the protrusion has an arc-shaped surface structure. The fourth chamber is set as a circular structure and equipped with protrusions arranged in an array around the fourth chamber. In this way, when the medium enters the circular fourth chamber, it can be guided by the circular structure and the arc-shaped structure of the protrusions to form a fluid with a regular flow rate and direction. The medium can form a vortex inside the fourth chamber after passing through multiple protrusions and be discharged from the outlet direction of the fourth chamber. In this process, the mixing effect of the medium is better to avoid the appearance of lumps in the medium causing blockage of the simulated blood vessel flow path. Based on the different flow rates inside the fourth chamber, different fourth chambers can form different output pressures to the first chamber and the third chamber. More importantly, the protrusion structure solves the problem that there may be turbulence in the medium input or short-term fluid irregularity that cannot simulate the real blood vessel state of the human body.

[0010] According to one embodiment of the present invention, a second chamber is provided between the first and third chambers, and the second chamber is connected to the first and third chambers via a first channel. The second chamber is configured to simulate multiple organs within the human body. The first and second chambers form multiple culture spaces, while the third chamber serves as a temporary storage space for medium discharge. Compared to existing technologies that use a single culture simulation space, the present invention provides more experimental options and greater accuracy.

[0011] According to one embodiment of the present invention, a collecting member connected to the third chamber is provided below the main body. The collecting member is a tubular structure and is used to collect waste liquid inside the third chamber. By arranging the collecting member below the third chamber, gravity is utilized to improve the waste liquid discharge efficiency inside the third chamber. The optional use of a pump body can control the waste liquid discharge rate inside the third chamber.

[0012] According to one embodiment of the present invention, a support body is disposed below the main body, and a support plate is disposed on one side of the main body, connected to the support body. The contact surface between the support body and the main body is flat, while the end surface facing away from the main body is inclined. The support body and the support plate ensure the flatness of the main body and prevent it from bending. Furthermore, the inclined surface of the support body can be mounted with a hydraulic rod to adjust the support body's tilt angle, thereby controlling the main body's tilt angle relative to the horizontal plane and providing different test parameter conditions.

[0013] According to one embodiment of the present invention, there are at least two bodies, adjacent bodies are spaced apart and arranged in an array, providing multiple test options while increasing the processing capacity.

[0014] A method for preparing a prestressed vascular chip is provided, wherein the steps of the preparation method are as follows:

[0015] Mask fabrication: Build a model of the target chip's positive film portion. Due to the differences in the upper and lower double-layer structures, two positive molds with different structural designs are required to produce a chip with different upper and lower layer structures. The positive film portion is prepared on the silicon wafer using a photolithography machine. Prepare the PDMS mixture: Mix the PDMS base liquid and crosslinking agent in appropriate proportions to obtain a uniform mixture.

[0016] Debubbling: Place the mixed PDMS mixture in a vacuum oven to remove air bubbles. Allow the mixture to rest under vacuum for a period of time to allow all air bubbles to escape. PDMS Coating: Apply the debubbled PDMS mixture evenly onto a silicon or glass wafer. Use a doctor blade or roller coating method to ensure a uniform and consistent coating thickness.

[0017] PDMS coating: evenly coat the de-bubbled PDMS mixture on a silicon wafer or glass sheet;

[0018] Prestressed fiber material laying: The fiber material is stretched and laid on the upper layer of PDMS, and the stretched state is fixed; various fiber materials are used to manufacture vascular prostheses. The fiber material is stretched to a certain extent, and the pre-stretched polymer fiber material is laid on the upper layer of PDMS, and the stretched state is fixed.

[0019] Baking and cross-linking: Place the PDMS-coated silicon wafer or glass wafer in an oven for baking and cross-linking;

[0020] Cutting the chip: After the PDMS is completely cross-linked, use a knife or laser cutting method to cut the PDMS chip into the desired shape and size.

[0021] Cleaning treatment: Place the two prepared PDMS chips into a plasma cleaning machine and bond the two PDMS chips to other materials to form a complete microfluidic vascular chip with a prestressed state.

[0022] The PDMS base liquid and the cross-linking agent are mixed in a ratio of 5-10:1. The preferred method is: Prepare the PDMS mixture: Mix the PDMS base liquid and the cross-linking agent in a ratio of 10:1 and mix thoroughly until a uniform mixture is obtained.

[0023] The cross-linking is carried out by baking at a temperature of about 80 degrees Celsius for 0.5-4 hours, preferably 2 hours.

[0024] By adding a material that resembles human soft tissue to the periphery of the vascular channel, this invention can simulate the external soft tissue constraints of the vascular channel when it deforms. This solves the problem that existing microfluidic chip technology cannot display vascular deformation. By adding pre-stretched linear polymer material to the area surrounding the blood vessel, the present invention can change the configuration of the vascular channel by stretching the wire.

[0025] Compared with the prior art, the present invention has the following beneficial effects: the present invention designs a first chamber for cell culture or testing, and provides a first channel for simulating blood vessels. By setting at least two fourth chambers, when the culture fluid or medium is input, the output pressure of the medium to the first chamber and the third chamber after passing through the fourth chamber is different. The flow rate of the medium entering each fourth chamber through the connecting channel is different, and the pressure and flow rate of the medium flowing out of these fourth chambers are different. In this way, the first channel and the subsequent flow path are subjected to different pressures, simulating the different reactions of blood vessels under different media and different flow rates, so that the medium input is close to the real difference in blood vessel status.

[0026] The vascular chip constructed by the present invention can achieve a state with mechanical performance morphology similar to the mechanical properties of blood vessels in the body, thereby solving the problem that the mechanical performance state of the vascular channels inside the chip of the prior art is greatly different from the actual blood vessel state of the human body. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] To more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for the embodiments or the description of the prior art. Obviously, the drawings described below are merely exemplary, and those skilled in the art can derive other implementation drawings based on the provided drawings without inventive effort.

[0028] Figure 1 This is a schematic diagram of a prestressed vascular chip assembly in use according to the present invention;

[0029] Figure 2 This is a schematic diagram of a prestressed vascular chip according to the present invention;

[0030] Figure 3 This is a schematic diagram of the fourth chamber on the body of the present invention;

[0031] Figure 4 A top view of the fourth chamber on the body of the present invention;

[0032] Figure 5 A schematic diagram of a connector solution of the present invention;

[0033] Figure 6 is a cross-sectional view of a second connection base of the present invention;

[0034] Figure 7 It is a schematic diagram of the position limiting member solution of the present invention;

[0035] Figure 8 It is a cross-sectional view of the limiting member of the present invention.

[0036] Description of reference numerals:

[0037] 10. Main body; 11. Collecting piece; 12. Third chamber; 13. Second chamber; 14. First chamber; 15. First channel; 16. Support body; 17. Extension plate; 18. Fourth chamber; 19. Protrusion; 20. Connecting piece; 21. First connecting base; 221. First hole body; 22. Second connecting base; 23. Second hole body; 24. Ring groove; 25. Third hole body; 26. Fourth hole body; 30. First tube body; 40. Limiting piece; 41. Fifth hole body; 42. Groove body; 43. Limiting inner tube; 44. Filling ring. DETAILED DESCRIPTION

[0038] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0039] The following first describes the concepts involved in this application with reference to the accompanying drawings. It should be noted that the following description of each concept is intended only to make the content of this application easier to understand and does not limit the scope of protection of this application. At the same time, the embodiments and features in the embodiments of this application can be combined with each other unless there is a conflict. The following detailed description of this application will be made with reference to the accompanying drawings and in conjunction with the embodiments.

[0040] Example 1

[0041] like Figure 1-8As shown, a prestressed vascular chip includes a main body 10, a first chamber 14 and a third chamber 12 are provided on the surface of the main body 10, the first chamber 14 is connected to the third chamber 12 through a first channel 15, the main body 10 is provided with at least two fourth chambers 18, the fourth chamber 18 is provided on one side of the first chamber 14, and there is a connecting channel between the fourth chambers 18, and at least two first channels 15 connected to the first chamber 14 are provided on the connecting channel. The present invention designs a first chamber 14 for cell culture or testing, and a first channel 15 for simulating blood vessels. By setting at least two fourth chambers 18, when the culture fluid or medium is input, the output pressure of the medium to the first chamber 14 and the third chamber 12 after passing through the fourth chamber 18 is different. The flow rate of the medium entering each fourth chamber 18 through the connecting channel is different, and the pressure and flow rate of the medium flowing out of these fourth chambers 18 are different. In this way, the first channel 15 and the subsequent flow path are subjected to different pressures, simulating the different reactions of blood vessels under different media and different flow rates, so that the medium input is close to the real difference in blood vessel status.

[0042] At the same time, the above design can provide multiple liquid inlet solutions, and it is also possible to choose to retain only a single fourth chamber 18 and a single connecting channel. The specific data can be freely selected according to the experimental purpose, and the experimental application range is relatively wide.

[0043] The main body 10 has an extension plate 17 on its side, to which the first tube 30 is connected, creating a communication channel between the first tube 30 and the fourth chamber 18. The first tube 30 is a capillary tube that supplies medium to the fourth chamber 18. The provision of the extension plate 17 stabilizes the input at the interface between the first tube 30 and the fourth chamber 18, resolving the problem of inflow flow rate, flow rate, and pressure being inconsistent with the pump input flow rate, flow rate, and pressure due to a bend in the first tube 30 and the fourth chamber 18.

[0044] The fourth chamber 18 is a circular chamber, with protrusions 19 arranged in an array at intervals around it. The sides of the protrusions 19 have an arcuate surface structure. By configuring the fourth chamber 18 as a circular structure and equipping it with protrusions 19 arranged in an array around it, the medium entering the circular fourth chamber 18 is guided by the circular structure and the arcuate surface structure of the protrusions 19 to form a fluid with a regular flow rate and direction. Passing through the multiple protrusions 19, the medium forms a vortex within the fourth chamber 18 and is discharged from the outlet of the fourth chamber 18. This process improves the mixing of the medium and prevents the presence of lumps in the medium that could block the simulated blood vessel flow path. Based on the different flow rates within the fourth chamber 18, different fourth chambers 18 can produce different output pressures to the first chamber 14 and the third chamber 12. More importantly, the protrusions 19 structure address the potential for turbulent flow or short-term fluid irregularities in the medium input that cannot simulate the actual state of human blood vessels.

[0045] A second chamber 13 is located between the first chamber 14 and the third chamber 12. The second chamber 13 communicates with both the first chamber 14 and the third chamber 12 via a first channel 15. The second chamber 13 is designed to simulate multiple organs within the human body. The first and second chambers 14, 13 form multiple culture spaces, while the third chamber 12 serves as a temporary storage space for media discharge. Compared to existing techniques that simulate a single culture space, the present invention offers more experimental options and greater accuracy.

[0046] A collecting piece 11 connected to the third chamber 12 is provided below the main body 10. The collecting piece 11 is a tubular structure and is used to collect waste liquid inside the third chamber 12. By arranging the collecting piece 11 below the third chamber 12, gravity is utilized to improve the waste liquid discharge efficiency inside the third chamber 12. The optional use of a pump body can control the waste liquid discharge rate inside the third chamber 12.

[0047] A support body 16 is located below the body 10, and a support plate connected to the support body 16 is located on one side of the body 10. The contact surface of the support body 16 with the body 10 is flat, while the end surface facing away from the body 10 is inclined. The support body 16 and the support plate ensure the flatness of the body 10 and prevent it from bending. Furthermore, the inclined surface of the support body 16 can be mounted with a hydraulic rod to adjust the tilt angle of the support body 16 and thus control the tilt angle of the body 10 relative to the horizontal plane, providing different test parameter conditions.

[0048] There are at least two main bodies 10 , and adjacent main bodies 10 are spaced apart and arranged in an array, providing multiple test options while increasing the processing capacity.

[0049] A method for preparing a prestressed vascular chip is provided, wherein the steps of the preparation method are as follows:

[0050] Mask fabrication: Build a model of the target chip's positive film portion. Due to the differences in the upper and lower double-layer structures, two positive molds with different structural designs are required to produce a chip with different upper and lower layer structures. The positive film portion is prepared on the silicon wafer using a photolithography machine. Prepare the PDMS mixture: Mix the PDMS base liquid and crosslinking agent in appropriate proportions to obtain a uniform mixture.

[0051] Debubbling: Place the mixed PDMS mixture in a vacuum oven to remove air bubbles. Allow the mixture to rest under vacuum for a period of time to allow all air bubbles to escape. PDMS Coating: Apply the debubbled PDMS mixture evenly onto a silicon or glass wafer. Use a doctor blade or roller coating method to ensure a uniform and consistent coating thickness.

[0052] PDMS coating: evenly coat the de-bubbled PDMS mixture on a silicon wafer or glass sheet;

[0053] Laying of prestressed fiber materials: The fiber material is stretched and laid on the upper layer of PDMS, and the stretched state is fixed. Various textile materials and fiber materials are used to manufacture vascular prostheses, including nylon polyurethane or spandex, polyvinyl acetal (Ivalon®) and acrylic (Orlon®), polyethylene terephthalate (PET) or polyester (Dacron®) and polytetrafluoroethylene or PTFE (Tefl on®) braided or knitted textile tubes or porous expanded membrane structures. The fiber material is stretched to a certain extent, and the pre-stretched polymer fiber material is laid on the upper layer of PDMS, and the stretched state is fixed.

[0054] Baking and cross-linking: Place the PDMS-coated silicon wafer or glass wafer in an oven for baking and cross-linking;

[0055] Cutting the chip: After the PDMS is completely cross-linked, use a knife or laser cutting method to cut the PDMS chip into the desired shape and size.

[0056] Cleaning treatment: Place the two prepared PDMS chips into a plasma cleaning machine and bond the two PDMS chips to other materials to form a complete microfluidic vascular chip with a prestressed state.

[0057] The PDMS base liquid and the cross-linking agent are mixed in a ratio of 5-10:1. The preferred method is: Prepare the PDMS mixture: Mix the PDMS base liquid and the cross-linking agent in a ratio of 10:1 and mix thoroughly until a uniform mixture is obtained.

[0058] The crosslinking was performed by baking at a temperature of about 80 degrees Celsius for 0.5-4 hours.

[0059] Example 2:

[0060] See attached Figure 2 , Attachment Figure 5 , Attachment Figure 6 As shown, this embodiment provides a further solution based on the actual example 1. A connector 20 is connected to the top of the fourth chamber 18 through a sealing ring. The connector 20 includes a second connecting base 22 connected to the inner wall of the upper end of the fourth chamber 18. The first connecting base 21 is coaxially assembled on the upper part of the second connecting base 22. Both the first connecting base 21 and the second connecting base 22 are cylindrical structures.

[0061] A sealing ring structure is provided at the connection between the second connecting base 22 and the fourth chamber 18. A first hole 221 is provided in the middle of the second connecting base 22 and does not pass through the bottom of the second connecting base 22. A fourth hole 26 connected to the fourth chamber 18 is provided at the bottom of the second connecting base 22. The first connecting base 21 has a column that is plugged into the first hole 221, and another first hole 221 connected to the first hole 221 on the second connecting base 22 is provided on the plugged column.

[0062] The second connection base 22 is surrounded by a second hole 23 , which is arranged around the center of the first hole 221 on the second connection base 22 . The second connection base 22 is provided with a through hole for connecting the second hole 23 with the first hole 221 .

[0063] The bottom surface of the second connection base 22 at the bottom of the second hole 23 is uniformly distributed with micropores.

[0064] An annular groove 24 is arranged around the outside of the second connecting base 22. When medium replenishment or other fluid input is needed, the medium can be directly input into the fourth chamber 18 by inserting the pipeline into the first hole body 221. The pipe body can also be matched with the sealing ring to cover the outside of the connecting piece 20 so that the sealing ring is filled in the annular groove 24 to prevent leakage of the pipe body. The medium can also be input into the first hole body 221 and then discharged into the fourth chamber 18 below from the fourth hole 26 and the micropores at the bottom of the second hole 23. This design can make the input medium enter the fourth chamber 18 in a diverted manner, solving the problem of turbulence caused by the convergence of the medium input into the chamber and the original rectified fluid in the fourth chamber 18. In addition, this solution can weaken the downward impact force of the supplementary input medium and the problem of excessive pressure difference between the interface of the connecting piece 20 and the fourth chamber 18. Specifically, the pressure entering the fourth chamber 18 is reduced by diverting the medium from the fourth hole 26 and the third hole body 25, thereby solving the pressure difference problem at the contact port.

[0065] Example 3:

[0066] See attached Figure 1 , Attachment Figure 7 , Attachment Figure 8 As shown, this embodiment provides a further solution based on the substantial example 1. The main body 10 of the present invention can be multiple. In this case, the number of the first tubes 30 increases accordingly. There may be problems such as the first tubes 30 being staggered or the pipelines being entangled with other equipment pipelines. For this reason, the attached Figure 7A limiting member 40 is provided to constrain multiple first tube bodies 30. The limiting member 40 includes a main body of its cylindrical sleeve structure, which is hollow inside and has limiting inner tubes 43 spaced apart. The outer wall of the limiting inner tube 43 is connected to the inner wall of the outer cylindrical sleeve structure body through a filling ring 44. On this basis, a coaxial fifth hole 41 is provided through the middle of the cylindrical sleeve structure body and the limiting inner tube 43, and a groove 42 is provided on one side of the cylindrical sleeve structure body and the limiting inner tube 43. Through the above-mentioned design scheme, multiple first tube bodies 30 can be placed in the fifth hole 41, thereby constraining the layout of multiple first tube bodies 30. The increase in the number of tube bodies may cause the diameter of the tube body to be compressed. The groove 42 provided in the present invention can provide the cylindrical sleeve structure body and the limiting inner tube 43 with a certain deformation space after the groove is opened. In this way, after the number of first tube bodies 30 increases, the cylindrical sleeve structure body and the limiting inner tube 43 are deformed to provide appropriate space to avoid the diameter of the first tube body 30 being compressed.

[0067] The embodiments and / or implementation methods described above are only used to illustrate the preferred embodiments and / or implementation methods for realizing the technology of the present invention, and do not impose any form of limitation on the implementation methods of the technology of the present invention. Any person skilled in the art may make slight changes or modifications to other equivalent embodiments without departing from the scope of the technical means disclosed in the content of the present invention, but they should still be regarded as technologies or embodiments that are essentially the same as the present invention.

[0068] This article uses specific examples to illustrate the principles and implementation methods of this application. The description of the above embodiments is only used to help understand the method and core ideas of this application. The above is only the preferred implementation method of this application. It should be pointed out that due to the limitations of textual expression, there are objectively infinite specific structures. For ordinary technicians in this technical field, without departing from the principles of this application, they can also make several improvements, modifications or changes, and can also combine the above technical features in an appropriate manner; these improvements, modifications, changes or combinations, or the direct application of the inventive concept and technical solution to other occasions without improvement, should be regarded as the scope of protection of this application.

Claims

1. A prestressed vascular chip, comprising a body (10), wherein a first chamber (14) and a third chamber (12) are formed on a surface of the body (10), wherein the first chamber (14) is connected to the third chamber (12) through a first channel (15), and wherein: The body (10) is provided with at least two fourth chambers (18), the fourth chambers (18) are provided on one side of the first chamber (14), and a communication channel is provided between the fourth chambers (18), and the communication channel is provided with at least two first channels (15) communicating with the first chamber (14); The fourth chamber (18) is a circular chamber, and projections (19) are arranged in an array with a spacing therebetween in the fourth chamber (18), and the side surfaces of the projections (19) have an arc-shaped surface structure.

2. The prestressed vascular chip according to claim 1, characterized in that: An extension plate (17) is provided on the side of the main body (10), a first tube body (30) is connected to the extension plate (17), and a communication channel between the first tube body (30) and the fourth chamber (18) is connected.

3. The prestressed vascular chip according to claim 1, characterized in that: A second chamber (13) is provided between the first chamber (14) and the third chamber (12), and the second chamber (13) is communicated with the first chamber (14) and the third chamber (12) respectively through a first channel (15).

4. The prestressed vascular chip according to claim 1, characterized in that: A collecting member (11) communicating with the third chamber (12) is provided below the main body (10); the collecting member (11) is a tubular structure.

5. The prestressed vascular chip according to claim 1, characterized in that: A support body (16) is provided below the main body (10), and a support plate connected to the support body (16) is provided on one side of the main body (10). The contact surface between the support body (16) and the main body (10) is a flat surface, and the end surface facing away from the main body (10) is an inclined surface.

6. The prestressed vascular chip according to claim 1, characterized in that: There are at least two bodies (10), and adjacent bodies (10) are spaced apart and arranged in an array.

7. A prestressed vascular chip according to any one of claims 1 to 6, wherein the preparation steps are as follows: Mask fabrication: Build a model of the target chip's anode film portion, and use a photolithography machine to prepare the anode film portion on the silicon wafer. Prepare the PDMS mixture: Mix the PDMS base liquid and cross-linking agent in proportion to obtain a uniform mixture. Degassing treatment: Place the mixed PDMS mixture in a vacuum oven for degassing treatment; PDMS coating: evenly coat the de-bubbled PDMS mixture on a silicon wafer or glass sheet; Laying of prestressed fiber materials: Stretch the fiber materials, lay the pre-stretched fiber materials on the upper layer of PDMS, and fix the stretched state; Baking and cross-linking: Place the PDMS-coated silicon wafer or glass wafer in an oven for baking and cross-linking; Cutting the chip: After the PDMS is completely cross-linked, use a knife or laser cutting method to cut the PDMS chip.

8. The prestressed vascular chip according to claim 7, characterized in that: PDMS base liquid and cross-linking agent are mixed in a ratio of 5-10:

1.

9. The prestressed vascular chip according to claim 7, characterized in that: The crosslinking step is performed by baking at a temperature of about 80 degrees Celsius for 0.5-4 hours.

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