A high-resistant starch composition for improving diabetes and preparation method thereof

Abstract

The invention discloses a high-resistant starch composition for improving diabetes and a preparation method thereof, and relates to the field of biotechnology. The high-resistant starch composition includes the following components in parts by weight: 45-55 parts of high-resistant starch, 8-15 parts of lecithin, 0.5-1.5 parts of angelic acid ketone and 0.3-1 parts of methyl 4-hydroxycinnamate; the high-resistant starch is prepared by cross-linking reaction with straight-chain starch as raw material under the action of a cross-linking agent adipic acid. The present invention prepares a high-resistant starch by esterification reaction of adipic acid with hydroxyl groups in starch molecules, and combines it with lecithin, angelic acid ketone and methyl 4-hydroxycinnamate to prepare a high-resistant starch composition. It has been confirmed by effect verification experiments that the high-resistant starch composition can effectively improve the blood sugar metabolism regulation ability of patients with type 2 diabetes, thereby providing new technical support for the preparation of drugs to improve diabetes.

Description

Technical Field

[0001] The invention relates to the field of biotechnology, and in particular to a high-resistant starch composition for improving diabetes and a preparation method thereof. Background Art

[0002] Diabetes is a chronic disease characterized by hyperglycemia, caused by absolute or relative insulin deficiency and utilization disorders. The disease is mainly divided into three types: type 1, type 2 and gestational diabetes. The cause is mainly attributed to the combined effects of genetic and environmental factors, including decreased insulin secretion caused by islet cell dysfunction, or the body's insensitivity to insulin, or both, which prevents glucose in the blood from being effectively utilized and stored. Some diabetic patients and families have a clustering phenomenon of the disease. In addition, the incidence and prevalence of diabetes are on the rise worldwide.

[0003] The symptoms of diabetes are mainly manifested as "three mores and one less", namely, polydipsia, polyuria, polyphagia and weight loss. In addition, patients with a long course of the disease may develop chronic progressive lesions, functional impairment or even failure of tissues and organs such as eyes, kidneys, nerves, heart, and blood vessels, and may also cause acute and severe metabolic disorders. It is very necessary to develop new products that can improve diabetes. Summary of the invention

[0004] The purpose of the present invention is to provide a high-resistant starch composition for improving diabetes and a preparation method thereof, so as to solve the problems existing in the above-mentioned prior art. The high-resistant starch composition can effectively improve the blood sugar metabolism regulation ability of patients with type 2 diabetes.

[0005] To achieve the above object, the present invention provides the following solutions:

[0006] The present invention provides a high-resistant starch composition for improving diabetes, comprising the following components in parts by weight:

[0007] 45-55 parts of high-resistant starch, 8-15 parts of lecithin, 0.5-1.5 parts of angelica ketone and 0.3-1 parts of methyl 4-hydroxycinnamate;

[0008] The high-resistant starch is prepared by using straight-chain starch as a raw material through a cross-linking reaction under the action of adipic acid as a cross-linking agent.

[0009] Furthermore, the mass ratio of the amylose to the adipic acid is 100:(2-3).

[0010] Furthermore, the pH of the cross-linking reaction is 4.5.

[0011] Furthermore, the reaction temperature of the cross-linking reaction is 45° C. and the reaction time is 3 hours.

[0012] Preferably, the high-resistant starch composition comprises the following components in parts by weight:

[0013] 50 parts of high-resistant starch, 10 parts of lecithin, 1 part of angelica ketone and 0.5 parts of methyl 4-hydroxycinnamate.

[0014] The present invention also provides a method for preparing the above-mentioned high-resistant starch composition, comprising the step of uniformly mixing the high-resistant starch, the lecithin, the angelic acid ketone and the methyl 4-hydroxycinnamate to prepare the high-resistant starch composition.

[0015] The present invention also provides use of the high-resistant starch composition in preparing a drug for improving diabetes.

[0016] The present invention also provides a medicine for improving diabetes, wherein the active ingredient comprises the above-mentioned high-resistant starch composition.

[0017] The present invention also provides the use of the high-resistant starch composition in preparing health food that helps maintain healthy blood sugar levels.

[0018] The present invention also provides a health food that helps maintain a healthy blood sugar level, wherein the active ingredient comprises the high-resistant starch composition.

[0019] The present invention discloses the following technical effects:

[0020] Adipic acid is an organic dicarboxylic acid. The present invention prepares a high-resistant starch by esterifying adipic acid with hydroxyl groups in starch molecules, and combines the high-resistant starch with lecithin, angelic acid ketone and methyl 4-hydroxycinnamate to prepare a high-resistant starch composition. The effect verification experiment confirms that the high-resistant starch composition can effectively improve the blood sugar metabolism regulation ability of patients with type 2 diabetes, thereby providing new technical support for the preparation of drugs to improve diabetes. DETAILED DESCRIPTION

[0021] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0022] It should be understood that the terms described in the present invention are only for describing a particular embodiment and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. The intermediate value in any stated value or stated range, and each smaller range between any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0023] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0024] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to those skilled in the art. The present invention description and examples are exemplary only.

[0025] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0026] Terminology Note:

[0027] The angelica ketone referred to in this application has a CAS number of 37719-98-5 and a molecular formula of C 16 H 16 O 5 , the structural formula is as follows:

[0028]

[0029] Methyl 4-hydroxycinnamate, CAS No. 19367-38-5, molecular formula C 10 H 10 O 3 , the structural formula is as follows:

[0030]

[0031] Amylose is a polysaccharide chain with D-glucose groups connected by α-(1,4) glycosidic bonds. There are about 200 glucose groups in the molecule and the molecular weight is 1 to 2×10 5 , degree of polymerization 990, spatial conformation curled into a spiral, each turn has 6 glucose groups. In addition to the sugar chain of α-(1,4) glycosidic bond, the branched starch molecule also has α-(1,6) glycosidic bond connected branches, containing 300 to 400 glucose groups in the molecule, with a molecular weight>2×10 7 , degree of polymerization 7200, each branch is also curled into a spiral. Starch reacts with iodine to form a color reaction, with amylose being blue and amylopectin being reddish brown.

[0032] Adipic acid is a dicarboxylic acid with the molecular formula C6 H 10 O 4 , the carboxyl group of adipic acid reacts with the hydroxyl group in the starch molecule under certain conditions (such as high temperature and the presence of a catalyst) to form an ester bond. This causes the starch molecules to be connected through adipic acid to form a network structure. The degree of cross-linking depends on factors such as the amount of adipic acid used, the reaction time and the temperature. Moderate cross-linking can increase the network structure of starch, making it more difficult to be decomposed by enzymes. After cross-linking, the starch is not easily decomposed by digestive enzymes in the human body (such as α-amylase) because it forms a tighter and more stable network structure, thereby improving its anti-digestion performance.

[0033] Example 1

[0034] 100 g of amylose (CAS No. 9005-82-7, purchased from Shanghai Kanglang Biotechnology Co., Ltd.), 150 g of deionized water and 10 g of Na 2 SO 4 Mix well, add 2.5g of crosslinking agent adipic acid (2-3g can achieve the same effect), and use H 2 SO 4 The solution was adjusted to pH 4.5, stirred at 45°C for 3 hours, and after the reaction was completed, the pH was adjusted to 6.5 with NaOH solution to terminate the reaction. The solution was then washed three times with distilled water, washed three times with anhydrous ethanol, and centrifuged to obtain a precipitate, dried at 40°C, crushed, and passed through a 100-mesh sieve to obtain high-resistant starch K-1.

[0035] Comparative Example 1

[0036] 100 g of amylose (CAS No. 9005-82-7, purchased from Shanghai Kanglang Biotechnology Co., Ltd.), 150 g of deionized water and 10 g of Na 2 SO 4 Mix well, add 2.5g of cross-linking agent sodium trimetaphosphate, adjust the pH to 10 with NaOH solution, stir at 45℃ for 3h, and add H 2 SO 4 The solution was adjusted to pH 6.5 to terminate the reaction, and then washed three times with distilled water, washed three times with anhydrous ethanol, centrifuged to obtain a precipitate, dried at a constant temperature of 40°C, crushed, and passed through a 100-mesh sieve to obtain high-resistant starch K-2.

[0037] Comparative Example 2

[0038] 100 g of amylose (CAS No. 9005-82-7, purchased from Shanghai Kanglang Biotechnology Co., Ltd.), 150 g of deionized water and 10 g of Na 2 SO 4Mix well, add 2.5g of cross-linking agent phosphorus oxychloride, adjust the pH to 10 with NaOH solution, stir at 45℃ for 3h, and add H 2 SO 4 The solution was adjusted to pH 6.5 to terminate the reaction, and then washed three times with distilled water, washed three times with anhydrous ethanol, centrifuged to obtain a precipitate, dried at a constant temperature of 40°C, crushed, and passed through a 100-mesh sieve to obtain high-resistant starch K-3.

[0039] Example 2

[0040] A high-resistant starch composition for improving diabetes, which is obtained by uniformly mixing the following components:

[0041] The high-resistant starch K-1 prepared in Example 1 was 50 g, lecithin was 10 g, angelic acid ketone was 1 g, and 4-hydroxycinnamic acid methyl ester was 0.5 g.

[0042] Example 3

[0043] A high-resistant starch composition for improving diabetes, which is obtained by uniformly mixing the following components:

[0044] The high-resistant starch K-15 prepared in Example 1 includes 55 g, 15 g of lecithin, 0.5 g of angelic acid ketone and 1 g of methyl 4-hydroxycinnamate.

[0045] Example 4

[0046] A high-resistant starch composition for improving diabetes, which is obtained by uniformly mixing the following components:

[0047] The high-resistant starch K-145 g, lecithin 8 g, angelica ketone 1.5 g and methyl 4-hydroxycinnamate 0.3 g prepared in Example 1.

[0048] Comparative Example 3

[0049] The same as Example 2, except that the high-resistant starch K-1 prepared in Example 1 is replaced by the high-resistant starch K-2 prepared in Comparative Example 1.

[0050] Comparative Example 4

[0051] The same as Example 2, except that the high-resistant starch K-1 prepared in Example 1 is replaced by the high-resistant starch K-3 prepared in Comparative Example 2.

[0052] Comparative Example 5

[0053] A high-resistant starch composition for improving diabetes, which is obtained by uniformly mixing the following components:

[0054] The high-resistant starch K-1 prepared in Example 1 was 50 g, lecithin was 10 g, and methyl 4-hydroxycinnamate was 0.5 g.

[0055] Comparative Example 6

[0056] A high-resistant starch composition for improving diabetes, which is obtained by uniformly mixing the following components:

[0057] The high-resistant starch K-1 prepared in Example 1 was 50 g, lecithin was 10 g, and angelica ketone was 1 g.

[0058] Example 5

[0059] 1. Experimental Materials

[0060] C57BL / 6 mice: 4 weeks old, weighing 18-20 g. During the feeding period, the animal room was set with a 12-h light-dark cycle, mice had free access to food and water, and the temperature was maintained between 21-25°C and the humidity was 50-70%.

[0061] 2.2 Type 2 diabetes mouse modeling method

[0062] The type 2 diabetes mouse model was established by feeding with a high-fat diet combined with intraperitoneal injection of streptozotocin (STZ). The successful criteria for the establishment of the type 2 diabetes mouse model were: mice with fasting blood glucose greater than 11.1mmol / L or random blood glucose greater than 16.7mmol / L and polyuria symptoms were determined to be type 2 diabetes mice.

[0063] 3. Experimental Grouping and Methods

[0064] C57BL / 6 mice were randomly divided into 7 groups, 20 mice in each group, and gavage and feeding were performed as shown in Table 1.

[0065] Table 1 Experimental groups

[0066] Grouping feed Oral gavage Example 2 High-fat diet + STZ The high-resistant starch composition prepared in Example 2 was administered intragastricly at a dose of 10 g / kg Comparative Example 3 High-fat diet + STZ The high-resistant starch composition prepared in Comparative Example 3 was administered intragastricly at a dosage of 10 g / kg Comparative Example 4 High-fat diet + STZ The high-resistant starch composition prepared by gavage in comparative example 4 was administered at a dosage of 10 g / kg Comparative Example 5 High-fat diet + STZ The high-resistant starch composition prepared by gavage in comparative example 5 was 10 g / kg Comparative Example 6 High-fat diet + STZ The high-resistant starch composition prepared by gavage in comparative example 6 was 10 g / kg Blank control Basic feed / Model Group High-fat diet + STZ /

[0067] Note: High-fat feed formula: basic feed 52.5% + lard 10% + cholesterol 2% + sucrose 30% + egg yolk powder 5% + bile salt 0.5%.

[0068] After continuous gavage and feeding for 4 weeks, each group of mice was given an oral glucose tolerance test, and the blood glucose level at 0 h was measured. 15 min later, 2.5 g / kg glucose solution was gavaged again, and the blood glucose levels of the mice were measured after waiting for 0.5 h, 1.0 h and 2.0 h.

[0069] 3. Experimental results

[0070] The blood glucose test results of each group of mice are shown in Table 2. The results showed that compared with the blank control group, the blood glucose levels of the model group mice were significantly increased after intragastric administration of the glucose solution (P<0.05). The blood glucose levels of the mice in the Example 2 group after intragastric administration of the glucose solution were significantly different from those of the mice in the model group (P<0.01), indicating that the high-resistant starch composition prepared in Example 2 can enhance the blood glucose metabolism regulation ability of type 2 diabetic mice.

[0071] The present invention attempts to prepare high-resistant starches with different cross-linking groups and finds that, compared with other cross-linking methods, high-resistant starch prepared by adipic acid cross-linking can significantly enhance the blood sugar metabolism regulation ability of type 2 diabetic mice.

[0072] Table 2 Blood glucose test results of mice in each group

[0073]

[0074] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A high-resistant starch composition for improving diabetes, characterized in that: The composition comprises the following components in parts by weight: 45-55 parts of high-resistant starch, 8-15 parts of lecithin, 0.5-1.5 parts of angelica ketone and 0.3-1 parts of methyl 4-hydroxycinnamate; The high-resistant starch is prepared by cross-linking reaction with amylose as raw material under the action of adipic acid as a cross-linking agent; The pH of the cross-linking reaction was 4.

5.

2. The high-resistant starch composition according to claim 1, characterized in that The mass ratio of the amylose to the adipic acid is 100:(2-3).

3. The high-resistant starch composition according to claim 1, characterized in that The reaction temperature of the cross-linking reaction is 45° C. and the reaction time is 3 hours.

4. The high-resistant starch composition according to claim 1, characterized in that The high-resistant starch composition comprises the following components in parts by weight: 50 parts of high-resistant starch, 10 parts of lecithin, 1 part of angelica ketone and 0.5 parts of methyl 4-hydroxycinnamate.

5. A method for preparing the high-resistant starch composition according to any one of claims 1 to 4, characterized in that: The method comprises the steps of uniformly mixing the high-resistant starch, the lecithin, the angelicone and the methyl 4-hydroxycinnamate to prepare the high-resistant starch composition.

6. Use of the high-resistant starch composition according to any one of claims 1 to 4 in the preparation of a drug for improving diabetes.

7. A drug for improving diabetes, characterized in that: The active ingredient comprises the high-resistant starch composition according to any one of claims 1 to 4.

8. Use of the high-resistant starch composition according to any one of claims 1 to 4 in the preparation of health food that helps maintain healthy blood sugar levels.

9. A health food that helps maintain healthy blood sugar levels, characterized in that: The active ingredient comprises the high-resistant starch composition according to any one of claims 1 to 4.

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