Extraction apparatus for phycoerythrin 545 extraction based on ultrasonic crushing method

Abstract

This invention discloses an extraction device for phycoerythrin 545 extract based on ultrasonic disruption, relating to the field of phycoerythrin extraction technology. The device includes a soundproof box with an ultrasonic generator module at its top and an amplitude transformer at its bottom output end. A limiting groove is formed inside the soundproof box, and a limiting plate is slidably connected within the groove. A support plate is located at the end of the limiting plate, and a cooling cylinder is located above the support plate. A sample container is detachably connected to the middle of the cooling cylinder. A lifting assembly for driving the support plate to rise and fall is located on the bottom inside the soundproof box, and a rotating assembly for driving the cooling cylinder to rotate is located on the top of the support plate. This invention achieves uniform sample distribution in the sample container, improves the disruption effect, and effectively ensures the cooling effect of the sample solution during use. Furthermore, it improves the cleaning efficiency of the amplitude transformer after use.

Description

Technical Field

[0001] This invention relates to the field of phycoerythrin extraction technology, specifically to an extraction device for phycoerythrin 545 extract based on ultrasonic disruption. Background Technology

[0002] Phycoerythrin is a fluorescent phycobiliprotein isolated and purified from red algae. As a fluorescent protein extracted from red algae, phycoerythrin has broad application prospects in biomedical research and clinical diagnosis. The extraction process of phycoerythrin usually includes steps such as raw material preparation, cell disruption, preliminary extraction, and purification. The cell disruption process is used to release phycoerythrin from red algal cells, and ultrasonic disruption is widely used in the cell disruption process.

[0003] Chinese patent application number 2017114829447 discloses an intelligent ultrasonic cell disruptor, including a shell, a sealed door, a disruption chamber, and an ultrasonic probe assembly. The sealed door has an observation window and a control module. The control module includes a touch screen, a microcomputer, a wireless communication module, a timing module, and a storage module that are electrically connected to each other. A lifting device is located in the center of the top of the disruption chamber. The ultrasonic probe assembly and a photoelectric sensor are connected below the lifting device. A condensation device and a temperature sensor are located at the bottom of the disruption chamber directly below the ultrasonic probe assembly. Three grooves are radially arranged around the condensation device and the temperature sensor. Each groove has a connected vertical baffle and a push-pull assembly above and below it. The three grooves are at a 120° angle to each other. However, during use, the sample in the sample container may be unevenly distributed, which will reduce the cell disruption effect.

[0004] In addition, during the cell disruption process, ice is usually used to cool the sample solution via an ice bath. However, during the cooling process, the ice near the sample container melts as the sample solution temperature rises, which can cause gaps to form between the ice and the sample container. The ice cannot directly contact the sample solution for heat exchange, reducing the cooling effect on the sample solution. Furthermore, after use, the amplitude transformer needs to be manually cleaned, which can easily lead to blind spots in cleaning and incomplete cleaning. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to overcome the existing defects and provide an extraction device for phycoerythrin 545 extract based on ultrasonic disruption, which can make the sample in the sample container evenly distributed, improve the disruption effect, and effectively ensure the cooling effect of the sample solution during use. In addition, the amplitude rod can be thoroughly cleaned after use, which can effectively solve the problems in the background technology.

[0006] To achieve the above objectives, the present invention provides the following technical solution: an extraction device for phycoerythrin 545 extract based on ultrasonic disruption, comprising a soundproof box, wherein an ultrasonic generating module is provided on the top of the soundproof box, and an amplitude transformer is provided at the bottom output end of the ultrasonic generating module;

[0007] The soundproof box has a limiting groove on its inner side, a limiting plate is slidably connected in the limiting groove, a support plate is provided at the end of the limiting plate, a cooling cylinder is provided above the support plate, and a sample container is detachably connected to the middle of the cooling cylinder.

[0008] The bottom of the soundproof box is provided with a lifting assembly for driving the support plate to rise and fall, the top of the support plate is provided with a rotating assembly for driving the cooling cylinder to rotate, and the inside of the soundproof box is provided with a cleaning assembly for cleaning the amplitude rod.

[0009] The cleaning component includes a slide rail, on which a bidirectional screw is rotatably connected. Both sides of the bidirectional screw are threaded with support arms. The ends of the support arms on both sides are provided with fixing rods on opposite sides. The opposite ends of the fixing rods on both sides are provided with arc-shaped seats. The inner side of the arc-shaped seats is provided with arc-shaped cleaning parts.

[0010] Preferably, the soundproof box has a soundproof layer inside, a sealed door is movably connected to the front of the soundproof box, soundproof glass is embedded in the middle of the sealed door, the annular space between the sample container and the cooling cylinder forms a cooling tank, and a temperature sensing probe is provided on the top inner side of the soundproof box.

[0011] Preferably, the lifting assembly includes a guide rail, a stud is rotatably connected inside the guide rail, a sliding seat is threaded onto the stud, a movable rod is movably connected to the top of the sliding seat, the top of the movable rod is movably connected to the bottom of the support plate, a lifting motor is provided at the end of the guide rail, and the output shaft of the lifting motor is fixedly connected to the end of the stud.

[0012] Preferably, the guide rail is located on the inner bottom surface of the soundproof box, and the sliding seat is slidably connected to the guide rail.

[0013] Preferably, the rotating assembly includes a rotating base and a rotating motor, wherein a gear ring is fixedly sleeved on the circumferential surface of the rotating base, and a rotating gear meshes with the side of the gear ring.

[0014] Preferably, the rotating seat is rotatably connected to the top of the support plate, the top of the rotating seat is fixedly connected to the bottom of the cooling cylinder, the rotating motor is mounted on the limiting plate, and the output shaft of the rotating motor is fixedly connected to the rotating gear.

[0015] Preferably, the slide rail is slidably connected to the inner side of the soundproof box, the two sides of the bidirectional screw are symmetrically provided with threads in opposite directions, the support arm is slidably connected to the slide rail, the inner top side of the soundproof box is provided with an electric telescopic rod, the telescopic end of the electric telescopic rod is fixedly connected to the top of the slide rail, the end of the slide rail is provided with a drive motor, and the output shaft of the drive motor is fixedly connected to the end of the bidirectional screw.

[0016] Preferably, the center of the arc-shaped cleaning part and the arc-shaped seat coincides with the central axis of the amplitude transformer rod. The arc-shaped cleaning part and the arc-shaped seat are connected by Velcro. The bottom of the arc-shaped seat on both sides is provided with a stirring rod, and the bottom end of the stirring rod on one side is provided with a lever at an angle.

[0017] Compared with the prior art, the present invention has the following advantages:

[0018] 1. After the crushing is completed, the support plate is placed at the bottom. The arc-shaped cotton pad is soaked in alcohol and fixed to the inside of the arc-shaped seat with Velcro. Then, the drive motor drives the bidirectional screw to rotate, causing the support arms on both sides to move in opposite directions until the arc-shaped cotton pads on both sides completely wrap the circumference of the amplitude rod. Then, the electric telescopic rod is used to move the arc-shaped cleaning part from top to bottom to wipe and clean the amplitude rod.

[0019] 2. After the sample container and ice are placed, the height of the arc-shaped seat is adjusted to be lower than the amplitude rod. Then, the arc-shaped seats on both sides move towards each other until the stirring rod is aligned with the sample container. The extension of the electric telescopic rod immerses the bottom of the stirring rod into the sample solution. The rotating component drives the cooling cylinder to rotate and the drive motor rotates slightly back and forth, so as to achieve uniform stirring of the sample solution by the stirring rod, while keeping the temperature of the middle part of the sample solution uniform with the outside temperature.

[0020] 3. In the process of breaking down red algae cells, when the sample solution temperature rises rapidly, the position of the stirring rod is adjusted to correspond with the cooling tank, and then the stirring rod is moved downwards to extend into the ice block. In this way, when the rotating component drives the cooling cylinder to rotate, the stirring rod on one side stirs the ice block, and the stirring rod on the other side drives the deflector to push the ice block to one side of the sample container, increasing the fit and stacking height of the ice block with the sample container, increasing the contact area between the ice block and the sample container, and improving the cooling efficiency of the sample solution. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of the structure of the present invention;

[0022] Figure 2 This is a schematic diagram of the internal structure of the present invention;

[0023] Figure 3 This is a schematic cross-sectional view of the present invention;

[0024] Figure 4 For the present invention Figure 3 Enlarged structural diagram at point A in the middle;

[0025] Figure 5 This is a front view structural diagram of the present invention.

[0026] In the diagram: 1. Soundproof box; 101. Sealed door; 102. Ultrasonic generating module; 103. Amplitude rod; 104. Limiting groove; 105. Limiting plate; 106. Support plate; 107. Cooling cylinder; 108. Sample container; 109. Temperature probe; 2. Lifting assembly; 201. Guide rail; 202. Sliding seat; 203. Stud; 204. Lifting motor; 205. Movable rod; 3. Rotating assembly; 301. Rotating seat; 302. Gear ring; 303. Rotating motor; 304. Rotating gear; 4. Cleaning assembly; 401. Slide rail; 402. Bidirectional screw; 403. Support arm; 404. Fixed rod; 405. Arc-shaped seat; 406. Arc-shaped cleaning part; 407. Stirring rod; 408. Electric telescopic rod; 409. Paddle plate; 410. Drive motor. Detailed Implementation

[0027] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0028] Please see Figures 1-5 This embodiment provides an extraction device for phycoerythrin 545 extract based on ultrasonic disruption, including a soundproof box 1. The soundproof box 1 has a sound insulation layer inside. A sealing door 101 is movably connected to the front of the soundproof box 1. Soundproof glass is embedded in the middle of the sealing door 101. An ultrasonic generating module 102 is provided on the top of the soundproof box 1. An amplitude transformer 103 is provided at the bottom output end of the ultrasonic generating module 102. The ultrasonic generating module 102 includes an ultrasonic generator and a transducer. The ultrasonic generator generates high-frequency electrical signals, which are transmitted to the transducer through a cable. The transducer converts the high-frequency electrical signals generated by the ultrasonic generator into mechanical vibrations. The vibrations generated by the transducer are effectively transmitted to the liquid through the amplitude transformer 103. It also includes an external control terminal electrically connected to the ultrasonic generating module 102 for adjusting various parameters during cell disruption.

[0029] A limiting groove 104 is provided on the inner side of the soundproof box 1. A limiting plate 105 is slidably connected in the limiting groove 104. A support plate 106 is provided at the end of the limiting plate 105. The cooperation between the limiting plate 105 and the limiting groove 104 is used to limit the support plate 106. A cooling cylinder 107 is provided above the support plate 106. A sample container 108 is detachably connected to the middle of the cooling cylinder 107. The bottom of the cooling cylinder 107 clamps and fixes the sample container 108 through a clamping structure. The clamping structure is horizontally set and is preferably a clamping mechanism commonly used for cylinders in the prior art. The annular space between the sample container 108 and the cooling cylinder 107 forms a cooling tank. The cooling tank is used to hold ice to cool the sample container 108 in an ice bath. A temperature sensor 109 is provided on the top inner side of the soundproof box 1. The temperature sensor 109 is used to detect the temperature of the sample solution during the crushing process.

[0030] The bottom of the soundproof box 1 is provided with a lifting assembly 2 for driving the support plate 106 to rise and fall. The lifting assembly 2 includes a guide rail 201, which is located on the bottom of the soundproof box 1. A stud 203 is rotatably connected inside the guide rail 201. A sliding seat 202 is threaded onto the stud 203. The sliding seat 202 is slidably connected to the guide rail 201. A movable rod 205 is movably connected to the top of the sliding seat 202. The top of the movable rod 205 is movably connected to the bottom of the support plate 106. A lifting motor 204 is provided at the end of the guide rail 201. The output shaft of the lifting motor 204 is fixedly connected to the end of the stud 203. The lifting motor 204 drives the stud 203 to rotate. The guide rail 201 limits the movement of the stud 203, causing the sliding seat 202 to move linearly. This causes the movable rod 205, which is in an inclined state, to gradually approach a vertical state. At the same time, the movable rod 205 pushes against the support plate 106 and moves upward, thereby realizing the lifting and lowering of the cooling cylinder 107 and the sample container 108.

[0031] The inner side of the soundproof box 1 is provided with a cleaning component 4 for cleaning the amplitude transformer 103. The cleaning component 4 includes a slide rail 401, which is slidably connected to the inner side of the soundproof box 1. A bidirectional screw 402 is rotatably connected inside the slide rail 401. The two sides of the bidirectional screw 402 are symmetrically provided with threads in opposite directions. Support arms 403 are threaded onto both sides of the bidirectional screw 402. The support arms 403 are slidably connected to the slide rail 401. A fixing rod 404 is provided on one side of the opposite end of the support arms 403 on both sides. Each of the two ends of 404 is provided with an arc-shaped seat 405. The inner side of the arc-shaped seat 405 is provided with an arc-shaped cleaning part 406. The end of the slide rail 401 is provided with a drive motor 410. The output shaft of the drive motor 410 is fixedly connected to the end of the bidirectional screw 402. The drive motor 410 drives the bidirectional screw 402 to rotate. The opposite threads on both sides cause the support arms 403 on both sides to move in opposite directions, so as to realize the opposite movement of the arc-shaped seats 405 and the arc-shaped cleaning part 406 on both sides, so that the arc-shaped cleaning part 406 is in close contact with the amplitude rod 103.

[0032] The arc-shaped cleaning part 406 is preferably an arc-shaped cotton pad soaked in alcohol. The center of the arc-shaped cleaning part 406 and the arc-shaped seat 405 coincides with the central axis of the amplitude rod 103, and the radius of the arc-shaped cleaning parts 406 on both sides is the same as that of the amplitude rod 103, so that when the arc-shaped cleaning part 406 is close to the amplitude rod 103, it completely covers the circumferential side of the amplitude rod 103. The arc-shaped cleaning part 406 and the arc-shaped seat 405 are connected by Velcro, which facilitates the disassembly and replacement of the arc-shaped cleaning part 406.

[0033] The top side of the soundproof box 1 is provided with an electric telescopic rod 408. The telescopic end of the electric telescopic rod 408 is fixedly connected to the top of the slide rail 401. The telescopic movement of the electric telescopic rod 408 drives the slide rail 401 to rise and fall, so as to adjust the height of the arc-shaped cleaning part 406.

[0034] In use, open the sealing door 101, place the sample container 108 in the middle of the cooling cylinder 107, and use the clamping structure to clamp and fix the container. Place the ice cube in the cooling tank, with the ice cube directly contacting the outer wall of the sample container 108. Start the lifting motor 204 to drive the stud 203 to rotate, causing the sliding seat 202 to slide away from the sealing door 101. The inclined movable rod 205 gradually approaches the vertical state and drives the support plate 106 to move the cooling cylinder 107 and the sample container 108 upward until the bottom end of the amplitude rod 103 is immersed in the sample solution to a specified length. Then, place the temperature probe 109 in the sample container 108 and close the sealing door 101. Next, adjust the various crushing parameters through the external control terminal, and then start the crushing program through the external control terminal. The amplitude rod 103 begins to vibrate.

[0035] After crushing, open the sealed door 101 and take out the sample container 108 and ice cubes in sequence. Lower the support plate 106 to the bottom and attach the arc-shaped cotton pad soaked in alcohol to the inner side of the arc-shaped seat 405 with Velcro. Start the drive motor 410 and use the rotation of the bidirectional screw 402 to drive the support arms 403 on both sides to move in opposite directions, so that the arc-shaped cleaning parts 406 on both sides wrap around the circumference of the amplitude rod 103. Then start the electric telescopic rod 408 to extend it, so that the arc-shaped cleaning parts 406 move along the amplitude rod 103 from high to low, completing one wipe of the amplitude rod 103. The number of wipes is preferably one to prevent the cleaned contaminants from re-adhering to the amplitude rod 103.

[0036] However, in actual use, the uneven distribution of samples within sample container 108 resulted in uneven contact area between ultrasound and cells during disruption, making it impossible to effectively and thoroughly disrupt the cell walls and cell membranes of red algae cells, thus reducing disruption efficiency. Furthermore, the ice cubes and sample solution required time for heat exchange, causing the temperature in the middle of the sample solution to drop more slowly. Therefore, the following improvements were made:

[0037] The top of the support plate 106 is provided with a rotating assembly 3 for driving the cooling cylinder 107 to rotate. The rotating assembly 3 includes a rotating seat 301 and a rotating motor 303. The rotating seat 301 is rotatably connected to the top of the support plate 106. The top of the rotating seat 301 is fixedly connected to the bottom of the cooling cylinder 107. A gear ring 302 is fixedly sleeved on the circumferential surface of the rotating seat 301. A rotating gear 304 meshes with the side of the gear ring 302. The rotating motor 303 is located on the limiting plate 105. The output shaft of the rotating motor 303 is fixedly connected to the rotating gear 304. The rotation of the rotating motor 303 drives the rotating gear 304 to rotate. The rotating gear 304 drives the rotating seat 301 to rotate through meshing with the gear ring 302, thereby realizing the rotation of the cooling cylinder 107 and the sample container 108.

[0038] A stirring rod 407 is provided at the bottom of the arc-shaped seats 405 on both sides.

[0039] In use, the sample container 108 containing the sample solution is placed in the middle of the cooling cylinder 107, and ice is placed in the cooling tank. At this time, the support plate 106 is at the bottom. First, the electric telescopic rod 408 drives the slide rail 401 to move downward, so that the height of the arc-shaped seat 405 is lower than the bottom of the amplitude rod 103. Then, the drive motor 410 is started, so that the arc-shaped seats 405 on both sides move in opposite directions, so that the stirring rods 407 on both sides correspond to the sample container 108 at the bottom. At this time, the arc-shaped seats 405 on both sides do not contact each other. The extension of the electric telescopic rod 408 is used to immerse the bottom of the stirring rod 407 into the sample solution. Next, the rotary motor 303 is started, and the gear ring 302 and the rotary gear 304 mesh to drive the rotation. The rotating seat 301 and the cooling cylinder 107 rotate, realizing the relative movement between the stirring rod 407 and the sample solution. At the same time, the drive motor 410 rotates back and forth in a small amplitude, causing the stirring rods 407 on both sides to move back and forth in a small amplitude along the radial direction of the cooling cylinder 107, thereby stirring the sample solution and making it more uniform. At the same time, it realizes rapid heat exchange between the middle and the outside of the sample solution, causing the temperature of the sample solution to drop rapidly and the temperature distribution of the sample solution to be more uniform. Therefore, before cell disruption, the present invention uses the cooperation between the rotating component 3 and the cleaning component 4 to stir the sample solution, ensuring the uniformity of the sample solution to improve the disruption effect, and causing the temperature in the middle of the sample solution to drop rapidly.

[0040] Furthermore, during the crushing process, the temperature of the sample solution gradually increases, and the ice near the outer wall of the sample container 108 melts preferentially. As the crushing time increases, the melting of the ice increases, causing the ice to fail to adhere to the outer wall of the sample container 108. Moreover, the height of the melting ice also decreases, which greatly reduces the cooling efficiency and causes the sample solution temperature to rise rapidly. Therefore, the following improvements are made:

[0041] One of the stirring rods 407 has a tilted baffle 409 at its bottom end. The tilt of the baffle 409 causes the ice to be pushed toward the sample container 108 when it rotates relative to the cooling cylinder 107.

[0042] During the disruption of red algae cells, if the temperature sensor 109 detects that the sample solution temperature is higher than the set threshold, it will actively determine that the temperature rise is accelerating and the cooling efficiency of the ice is decreasing, failing to meet the disruption requirements. At this time, the drive motor 410 is activated, causing the support arms 403 on both sides to move in opposite directions until the stirring rod 407 aligns with the cooling tank. At this point, the two arc-shaped seats 405 are not closed, and the distance between the arc-shaped seats 405 and the amplitude transformer 103 does not affect the normal operation of the amplitude transformer 103. Then, the electric telescopic rod 408 drives the slide rail 401 to move the stirring rod 407 downward, so that the stirring rod 407 is inserted into the ice. At the same time, the lever 409 on one side is also inserted into the ice in the cooling tank. The rotary motor 303 is activated, causing the rotary seat 301 to move downward. The rotating cooling cylinder 107 rotates, causing relative rotation between the ice block and the stirring rod 407, thus agitating the ice block. Simultaneously, one stirring rod 407 agitates the ice block, while the other stirring rod 407, under the action of the inclined paddle plate 409, propels the ice block towards one side of the sample container 108, ensuring close contact between the ice block and the sample container 108 and increasing the ice block's stacking height. This increases the contact area between the ice block and the sample container 108, ensuring efficient cooling of the sample solution. Therefore, in the cell disruption process, this invention utilizes the structural cooperation between the rotating component 3 and the cleaning component 4 to agitate and propel the ice block, increasing the contact area between the ice block and the sample container 108 and ensuring efficient cooling of the sample solution.

[0043] Simultaneously, the rotation of the sample container 108 driven by the cooling cylinder 107 enables the sample solution to rotate, making the bubbles generated in the sample solution under the vibration of the amplitude rod 103 more evenly distributed. This increases the contact area between the ultrasound and the sample, allowing the energy of the ultrasound to be transferred to the sample more effectively, improving the disruption efficiency. At the same time, it increases the shear force and impact force on the cell wall, further promoting the rupture of the red algae cell wall, releasing more phycoerythrin 545, and accelerating the heat exchange between the middle of the sample solution and the ice.

[0044] The above description is merely an embodiment of the present invention and does not limit the patent scope of the present invention. Any equivalent structural or procedural transformations made based on the content of the present invention specification and drawings, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of the present invention.

Claims

1. An apparatus for extracting phycoerythrin 545 extract based on ultrasonic disruption method, comprising a soundproof box, characterized in that, The top of the sound insulation box is provided with an ultrasonic generating module, and the bottom output end of the ultrasonic generating module is provided with a variable amplitude rod; The inner side of the sound insulation box is provided with a limiting groove, a limiting plate is slidably connected in the limiting groove, the end of the limiting plate is provided with a supporting plate, the upper side of the supporting plate is provided with a cooling cylinder, the middle part of the cooling cylinder is detachably connected with a sample container, and the annular space between the sample container and the cooling cylinder forms a cooling groove; The inner bottom side of the sound insulation box is provided with a lifting assembly for driving the supporting plate to lift, the top of the supporting plate is provided with a rotating assembly for driving the cooling cylinder to rotate, and the inner side of the sound insulation box is provided with a cleaning assembly for cleaning the variable amplitude rod; The cleaning assembly comprises a sliding rail, a bidirectional screw rod is rotationally connected in the sliding rail, supporting arms are threadedly connected on the two sides of the bidirectional screw rod, fixed rods are arranged on the end of the two supporting arms, arc-shaped seats are arranged on the opposite ends of the two fixed rods, and arc-shaped cleaning parts are arranged on the inner sides of the arc-shaped seats; The device further comprises a temperature sensing probe and a control system, the temperature sensing probe is arranged on the inner top side of the sound insulation box and is used for monitoring the temperature of the sample solution, and the control system automatically adjusts the working modes of the stirring rod and the stirring plate according to the temperature data; The supporting arms are slidably connected with the sliding rail, the bottom of the arc-shaped seat on the two sides is provided with a stirring rod, and the bottom end of the stirring rod on one side is obliquely provided with a stirring plate; the stirring rod and the stirring plate can be selectively immersed in the sample solution or inserted into the ice block in the cooling groove; in use, the height of the arc-shaped seat is lower than the bottom end of the variable amplitude rod, the driving motor is started, the arc-shaped seats on the two sides move towards each other, the stirring rods on the two sides correspond to the sample container at the bottom, at this time, the arc-shaped seats on the two sides do not contact each other, the lengthening of the electric telescopic rod is continued to immerse the bottom of the stirring rod in the sample solution, the rotating motor is then started, the rotating seat and the cooling cylinder are driven to rotate by the meshing of the gear ring and the rotating gear, the relative movement between the stirring rod and the sample solution is realized, at the same time, the driving motor is reciprocated at a small amplitude to make the stirring rods on the two sides reciprocate along the radial direction of the cooling cylinder at a small amplitude, and the sample solution is stirred; when the temperature sensing probe detects that the temperature of the sample solution is higher than a set threshold value, the driving motor is started to make the supporting arms on the two sides move towards each other until the stirring rods correspond to the position of the cooling groove, the electric telescopic rod is used to drive the sliding rail to drive the stirring rod to move downwards, the stirring rod is inserted into the ice block, at the same time, the stirring plate on one side is also inserted into the ice block in the cooling groove, the rotating motor is started to make the rotating seat drive the cooling cylinder to rotate, and the relative rotation between the ice block and the stirring rod is realized. The rotating assembly comprises a rotating seat and a rotating motor, the circumferential surface of the rotating seat is fixedly sleeved with a gear ring, the side surface of the gear ring is meshed with a rotating gear, and the rotating seat is rotationally connected to the top of the supporting plate and fixedly connected with the bottom of the cooling cylinder; The inner top side of the sound insulation box is provided with an electric telescopic rod, the telescopic end of the electric telescopic rod is fixedly connected with the top of the sliding rail, and the end of the sliding rail is provided with a driving motor, the output shaft of the driving motor is fixedly connected with the end of the bidirectional screw rod; The sliding rail is slidably connected to the inner side of the sound insulation box, the two sides of the bidirectional screw rod are symmetrically provided with threads with opposite screw directions, the centers of the arc-shaped cleaning parts and the arc-shaped seats coincide with the central axis of the variable amplitude rod; The inner top side of the sound insulation box is provided with an electric telescopic rod, the telescopic end of the electric telescopic rod is fixedly connected with the top of the sliding rail, and the end of the sliding rail is provided with a driving motor, the output shaft of the driving motor is fixedly connected with the end of the bidirectional screw rod; The lifting assembly comprises a guide rail, a stud rotatably connected in the guide rail, a sliding seat threadedly sleeved on the stud, a movable rod movably connected to the top of the sliding seat, the top of the movable rod movably connected to the bottom of a supporting plate, a lifting motor provided at the end of the guide rail, and an output shaft of the lifting motor fixedly connected to the end of the stud.

2. The apparatus for extracting phycoerythrin 545 based on ultrasonic disruption method according to claim 1, characterized in that: The soundproof box is internally provided with a soundproof layer, a sealing door movably connected to the front side of the soundproof box, soundproof glass embedded in the middle of the sealing door, and a temperature sensing probe provided at the top of the inner side of the soundproof box.

3. The apparatus for extracting phycoerythrin 545 based on ultrasonic disruption method according to claim 1, characterized in that: The rotary motor is provided on the limiting plate, and an output shaft of the rotary motor is fixedly connected to the rotary gear.

4. The apparatus for extracting phycoerythrin 545 based on ultrasonic disruption according to claim 1, wherein: The arc-shaped cleaning part and the arc-shaped seat are bonded by magic tape.

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