Dried blood spot quantification and dispensing device and method

Abstract

A device and method for quantitative dispensing of dried blood spots. The device comprises a substrate, a dried blood spot and an operating switch. The substrate comprises a first surface and a second surface oppositely arranged in a first direction. The substrate is provided with a recess and a slot, the recess is provided on the first surface or the second surface, and the slot is provided on the first surface or the second surface. The substrate is provided with an introduction channel and a plurality of capillary channels, each capillary channel has a predetermined volume, and the outlet end of each capillary channel is provided on the bottom surface of the recess. The operating switch is movably connected in the slot. The operating switch is used to switch the quantitative dispensing device of dried blood spots to a communication state and a blocking state respectively when operating. The communication state is that the introduction channel is connected to the plurality of capillary channels. The blocking state is that the introduction channel and the plurality of capillary channels are disconnected. The dried blood spot is provided in the recess and covers the outlet end of each capillary channel. The application can realize quantitative detection.

Description

Technical Field

[0001] This application relates to the field of biochemical detection, and in particular to a device and method for quantitative dispensing of dried blood spots. Background Technology

[0002] Sampling (such as blood sampling) is the first step in testing human health indicators. Safe, convenient, and effective sampling methods can not only protect the test subject from infection and harm during the sampling process, but also improve the accuracy of the subsequent tests. Blood transportation is a crucial step after blood sampling. Whole blood samples require cold chain transportation, which demands extremely high transport conditions, increases transportation costs, and makes it difficult to maintain long-term stability.

[0003] Dried blood spot (DBS) technology involves collecting whole blood samples onto a cardstock to form dried blood spots, which are then sent for testing. This method requires a small amount of blood; just a few to tens of microliters of blood forming appropriately sized circular dried blood spots on the cardstock is sufficient for testing. Infectious pathogens (such as HIV and hepatitis B virus) cannot survive in the dried blood spot environment, and the analyte remains stable within this environment, eliminating the need for cold chain transportation.

[0004] However, dried blood spots are usually collected non-quantitatively, which limits the application of dried blood spot technology in some situations that require quantitative detection (such as screening for metabolic diseases in newborns). Summary of the Invention

[0005] To address the above shortcomings, it is necessary to provide a device for quantitative dispensing of dried blood spots.

[0006] In addition, it is necessary to provide a method for quantitative dispensing of dried blood spots applied to the above-mentioned quantitative dispensing device for dried blood spots.

[0007] This application provides a dried blood spot quantitative dispensing device, including a substrate, an operating switch, and dried blood paper sheets. The substrate includes a first surface and a second surface disposed opposite to each other in a first direction. The substrate has a groove and a slot, each of which is independently formed on the first surface or the second surface. The substrate has an inlet channel and a plurality of capillary channels. The inlet channel is used to introduce a blood sample. Each capillary channel has a predetermined volume, and the outlet end of each capillary channel is located on the bottom surface of the groove. The operating switch is movably connected to the slot and is used to switch the dried blood spot quantitative dispensing device to a connected state and a blocked state during operation. In the connected state, the inlet channel connects to the plurality of capillary channels, allowing the blood sample introduced through the inlet channel to enter each capillary channel and, under capillary action, fill each capillary channel and extend to its outlet end. In the blocked state, the inlet channel and the plurality of capillary channels are disconnected. The dried blood paper is disposed in the groove and covers the outlet end of each capillary channel. The dried blood paper is used to adsorb the blood sample in the capillary channel through the outlet end of each capillary channel and form a dried blood spot.

[0008] In some possible implementations, the operating switch is rotatably disposed within the slot, and the operating switch has an intermediate channel. The operating switch is used to switch the dried blood spot quantitative dispensing device to the connected state and the blocked state when rotated. The connected state is that the intermediate channel is connected to the inlet channel and the multiple capillary channels respectively, and the blocked state is that the intermediate channel is disconnected from the inlet channel and the multiple capillary channels respectively.

[0009] In some possible implementations, the operating switch is slidably disposed within the slot along the first direction, and the operating switch has an intermediate channel. The operating switch is used to switch the dried blood spot quantitative dispensing device to the connected state and the blocked state when sliding along the first direction. In the connected state, the intermediate channel is connected to both the inlet channel and the multiple capillary channels; in the blocked state, the intermediate channel is disconnected from both the inlet channel and the multiple capillary channels.

[0010] In some possible implementations, the dried blood spot quantitative dispensing device further includes a seal surrounding the operating switch. When the dried blood spot quantitative dispensing device is in the connected state, the seal is offset from the outlet end of the inlet channel and the inlet end of each capillary channel, respectively. When the dried blood spot quantitative dispensing device is in the blocked state, the seal is sealingly connected to the outlet end of the inlet channel and the inlet end of each capillary channel.

[0011] In some possible implementations, the dried blood spot quantitative dispensing device further includes a buffer element disposed in the first direction between the operating switch and the bottom surface of the slot.

[0012] In some possible implementations, the operating switch is slidably disposed within the slot along a second direction perpendicular to the first direction. The outlet end of the inlet channel communicates with one end of the slot. The operating switch is used to switch the dried blood spot quantitative dispensing device to the connected state and the blocked state when sliding along the second direction. The connected state is characterized by a negative pressure generated in the space between the end of the slot and the operating switch, and the inlet channel communicating with a plurality of capillary channels through the space. The blocked state is characterized by the operating switch isolating the end and the plurality of capillary channels, thus disconnecting the inlet channel from the plurality of capillary channels.

[0013] In some possible implementations, the operating switch includes a main body and an operating part. The main body is disposed within the slot. The main body includes a first sealing part and a second sealing part disposed opposite to each other in the second direction, and a connecting part connecting the first sealing part and the second sealing part. The operating part is connected to the connecting part and protrudes from the slot. Both the first sealing part and the second sealing part are sealingly connected within the slot.

[0014] In some possible implementations, the substrate further includes a side surface connected between the first surface and the second surface, with the inlet end of the inlet channel located on the side surface. The dried blood spot quantitative dispensing device further includes a blood collector detachably mounted on the side surface and a hollow needle portion disposed within the inlet channel, the needle portion protruding from the inlet end of the inlet channel and extending into the blood collector.

[0015] In some possible implementations, the substrate further includes a side surface connected between the first surface and the second surface, with the inlet end of the introduction channel located on the side surface. The dried blood spot quantitative dispensing device also includes a blood collector integrally mounted on the side surface, with a negative pressure formed within the blood collector, and the inlet end of the introduction channel connected to the blood collector.

[0016] In some possible implementations, the dried blood paper has several blood spot sampling points, the outlet ends of multiple capillary channels are arranged along the same straight line and are directly opposite the blood spot sampling points, the volume of each capillary channel corresponds to the size of the blood spot sampling point, and at least one capillary channel is provided with a bent section.

[0017] In some possible implementations, the substrate is made of transparent plastic.

[0018] A second aspect of this application also provides a method for quantitative dispensing of dried blood spots using the aforementioned quantitative dispensing device, comprising: operating the operating switch to switch the quantitative dispensing device to a connected state, thereby connecting the inlet channel to a plurality of capillary channels; introducing a blood sample through the inlet channel, such that the blood sample fills each capillary channel under capillary action and extends to the outlet end of the capillary channel; operating the operating switch to switch the quantitative dispensing device to a blocked state, thereby disconnecting the inlet channel from the plurality of capillary channels; and adsorbing the blood sample in the capillary channels by the suction of the dried blood paper, such that the blood sample flows through the outlet end of the capillary channel to the dried blood paper and forms a dried blood spot.

[0019] In the dried blood spot quantitative dispensing device of this application, the capillary channel is set to a predetermined volume according to actual needs. The operation switch is operated to put the dried blood spot quantitative dispensing device in the connected state. At this time, the blood sample flowing in through the inlet end of the inlet channel fills each capillary channel under the action of capillary and extends to the outlet end of the capillary channel. At this time, the blood is quantitatively collected in the capillary channel. Then, the operation switch is operated to put the dried blood spot quantitative dispensing device in the blocked state, disconnecting the blood source. The blood in the capillary channel is printed onto the dried blood paper sheet through the outlet end of the capillary channel under the suction action of the dried blood paper sheet, forming dried blood spots, thereby completing the quantitative collection of blood samples with high quantitative accuracy. Attached Figure Description

[0020] Figure 1 This is a three-dimensional structural schematic diagram of a dried blood spot quantitative dispensing device provided in one embodiment of this application.

[0021] Figure 2 for Figure 1 The diagram shows a three-dimensional structure of the dried blood spot quantitative dispensing device from another angle.

[0022] Figure 3 for Figure 1 An exploded view of the dried blood spot quantitative dispensing device shown.

[0023] Figure 4 for Figure 1 The diagram shows a cross-sectional view of the dried blood spot quantitative dispensing device along IV-IV.

[0024] Figure 5 for Figure 4 The diagram shows a cross-sectional view of the dried blood spot quantitative dispensing device after the operating switch has been turned.

[0025] Figure 6 for Figure 4 The diagram shows a cross-sectional view of the dried blood spot quantitative dispensing device after the operation switch has been pressed.

[0026] Figure 7 for Figure 1 The diagram shows a top view of the dried blood spot quantitative dispensing device after the first cover of the substrate has been removed.

[0027] Figure 8 for Figure 7 The diagram shows a top view of the dried blood spot quantitative dispensing device after the operating switch has been turned.

[0028] Figure 9 A three-dimensional structural schematic diagram of a dried blood spot quantitative dispensing device provided for another embodiment of this application.

[0029] Figure 10 for Figure 9 An exploded view of the dried blood spot quantitative dispensing device shown.

[0030] Figure 11 for Figure 9 The diagram shows a cross-sectional view of the dried blood spot quantitative dispensing device along XI-XI.

[0031] Figure 12 for Figure 11 The diagram shows a cross-sectional view of the dried blood spot quantitative dispensing device after the operating switch has been slid.

[0032] Figure 13 A three-dimensional structural schematic diagram of a dried blood spot quantitative dispensing device provided in another embodiment of this application.

[0033] Figure 14 for Figure 13 The diagram shows a cross-sectional view of the dried blood spot quantitative dispensing device along XIV-XIV.

[0034] Figure 15 for Figure 14 The diagram shows a cross-sectional view of the dried blood spot quantitative dispensing device after the operating switch has been slid.

[0035] Figure 16 A flowchart of a method for quantitative dispensing of dried blood spots provided in one embodiment of this application.

[0036] Explanation of main component symbols

[0037] Substrate 10

[0038] First cover 10a

[0039] Second cover 10b

[0040] First surface 11

[0041] Second surface 12

[0042] Side view 13

[0043] Channel 14

[0044] Capillary channel 15

[0045] 20 pieces of dried blood paper

[0046] Blood spot sampling point 21

[0047] Operation switch 30

[0048] Middle passage 31

[0049] Main body 32

[0050] Third cover 32a

[0051] Fourth cover 32b

[0052] First sealing part 32c

[0053] Second sealing part 32d

[0054] Connecting part 32e

[0055] Operation Section 33

[0056] Seal 40

[0057] Buffer 50

[0058] Dried blood spot quantitative dispensing device 100, 200, 300

[0059] Grooving 110

[0060] Groove 120

[0061] Bottom 111, 121

[0062] Area 131

[0063] Second area 132

[0064] Third Region 133

[0065] Fourth area 134

[0066] Fifth District 135

[0067] Area 6, 136

[0068] First entrance 141

[0069] First exit end 142

[0070] Acupuncture point 143

[0071] Blood collector 144

[0072] Third entrance 151

[0073] Third exit end 152

[0074] Second entrance end 311

[0075] Second exit end 312

[0076] Opening 1100

[0077] First opening section 1100a

[0078] Second opening section 1100b

[0079] Third opening section 1100c

[0080] First direction X

[0081] Second direction Y

[0082] Third direction Z

[0083] The following detailed description, in conjunction with the accompanying drawings, will further illustrate this application. Detailed Implementation

[0084] The technical solutions in the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments.

[0085] It should be noted that when a component is considered "set on" another component, it can be directly set on the other component or may have an intervening component present; when a component is considered "installed on" another component, it can be directly installed on the other component or may have an intervening component present. The term "and / or" as used herein includes all and any combination of one or more of the related listed items.

[0086] Please see Figures 1 to 3 This application provides a dried blood spot quantitative dispensing device 100, which can be applied to occasions requiring quantitative detection, such as screening for newborn metabolic diseases, collection of infectious disease samples, doping detection in athletes, and detection of biomarkers. The dried blood spot quantitative dispensing device 100 includes a substrate 10, dried blood paper sheets 20, and an operating switch 30.

[0087] The substrate 10 includes a first surface 11, a second surface 12, and a side surface 13. The first surface 11 and the second surface 12 are disposed opposite each other in a first direction X. The side surface 13 connects the first surface 11 and the second surface 12. A portion of the second surface 12 is recessed toward the first surface 11 to form a groove 120. The groove 120 does not penetrate the first surface 11, that is, the bottom surface 121 of the groove 120 (in) Figure 4(As shown in the diagram) It is disposed between the first surface 11 and the second surface 12 in the first direction X. The dried blood paper strip 20 is disposed within the groove 120. (See diagram) Figure 3 As shown, the dried blood paper 20 has multiple blood spot sampling points 21. In some other embodiments, the groove 120 may also be provided on the first surface 11.

[0088] Please refer to the above as well. Figure 4 and Figure 7 The substrate 10 has an inlet channel 14 and a plurality of capillary channels 15 that can communicate with the inlet channel 14. The inlet channel 14 includes a first inlet end 141 and a first outlet end 142 disposed opposite to each other, with the first inlet end 141 formed on the side 13. In some embodiments, the substrate 10 includes a first cover 10a and a second cover 10b stacked in a first direction X. The first cover 10a includes a first surface 11, and the second cover 10b includes a second surface 12. The inlet channel 14 and the capillary channels 15 may both be formed on the side of the second cover 10b facing the first cover 10a. The first cover 10a covers the second cover 10b, thereby closing the inlet channel 14 and the capillary channels 15 in the first direction X. Further, in some embodiments, the groove 120 does not penetrate the second cover 10b. Figure 1 and Figure 3 As shown, in some embodiments, side 13 includes a first region 131, a second region 132, a third region 133, a fourth region 134, a fifth region 135, and a sixth region 136 connected end-to-end. The third region 133 and the fifth region 135 are arranged opposite each other in a second direction Y perpendicular to the first direction X, and the third region 133 may be parallel to the fifth region 135. The fourth region 134 is perpendicularly connected between the third region 133 and the fifth region 135, and the first region 131 may be parallel to the fourth region 134. The second region 132 and the sixth region 136 may be obliquely connected to the first region 131. In the second direction Y, the size of the first region 131 is smaller than the size of the fourth region 134. The first entrance end 141 of the introduction channel 14 is formed in the first region 131.

[0089] like Figure 3 and Figure 4 As shown, a portion of the first surface 11 is recessed toward the second surface 12 to form a slot 110. The slot 110 does not penetrate the second surface 12. An operating switch 30 is movably connected within the slot 110 of the substrate 10. In a third direction Z, perpendicular to both the first direction X and the second direction Y, the operating switch 30 is disposed between the inlet channel 14 and each capillary channel 15. More specifically, in the third direction Z, the operating switch 30 is disposed between the first outlet end 142 of the inlet channel 14 and each capillary channel 15. An intermediate channel 31 is provided within the operating switch 30. The intermediate channel 31 includes a second inlet end 311 and a plurality of second outlet ends 312. Figure 4 , Figure 5 , Figure 7 and Figure 8 As shown, the operating switch 30 is used to switch the dried blood spot quantitative dispensing device 100 to an on / off state and an off state respectively during operation. Wherein, as... Figure 4 and Figure 7 As shown, in the connected state, the second inlet end 311 is connected to the first outlet end 142 of the inlet channel 14, and the multiple second outlet ends 312 are respectively connected to multiple capillary channels 15. Therefore, when the dried blood spot quantitative dispensing device 100 is in the connected state, the blood sample introduced through the inlet channel 14 can enter the multiple capillary channels 15 through the intermediate channel 31. Figure 5 and Figure 8 As shown, the blocking state is characterized by the second inlet end 311 being disconnected from the inlet channel 14, and the multiple second outlet ends 312 being disconnected from the multiple capillary channels 15 respectively. Therefore, when the dried blood spot quantitative dispensing device 100 is in the blocking state, the blood sample introduced through the inlet channel 14 is blocked by the operation switch 30, preventing the blood sample from entering the multiple capillary channels 15 through the intermediate channel 31. In other embodiments, the slot 110 may also be provided on the second surface 12.

[0090] In some embodiments, the operating switch 30 is rotatably disposed within the slot 110, and when rotated, the operating switch 30 switches the dried blood spot quantitative dispensing device 100 to an on / off state and an off / open state. For example... Figure 7 and Figure 8 As shown, when the dried blood spot quantitative dispensing device 100 is switched from the connected state to the blocked state, the rotation angle of the operating switch 30 can be approximately 90 degrees. Please refer to the following: Figure 4 and Figure 5 In other embodiments, the operating switch 30 can also be slidably disposed within the slot 110 along the first direction X. In this case, the operating switch 30 can also be slid along the first direction X (e.g., pressed), which similarly disconnects the second inlet end 311 from the inlet channel 14. For example, Figure 3 and Figure 4 As shown, the operating switch 30 includes a main body portion 32 and an operating portion 33 connected in the first direction X. The main body portion 32 is disposed within the substrate 10, and an intermediate channel 31 is disposed within the main body portion 32. The operating portion 33 protrudes from the first surface 11 of the substrate 10, and the operator can rotate or press the operating switch 30 by operating the operating portion 33. Further, in some embodiments, the main body portion 32 may include a third cover 32a and a fourth cover 32b stacked in the first direction X, and the operating portion 33 is connected to the third cover 32a. A plurality of capillary channels 15 may be evenly disposed on the side of the fourth cover 32b facing the third cover 32a. The third cover 32a covers the fourth cover 32b, thereby closing the plurality of capillary channels 15 in the first direction X.

[0091] Each capillary channel 15 is set to a predetermined volume according to actual needs to facilitate quantitative blood collection. The volumes of each capillary channel 15 can be the same or different. Figure 4 , Figure 5 , Figure 7 and Figure 8 As shown, each capillary channel 15 includes a third inlet end 151 and a third outlet end 152 disposed opposite to each other. Each third inlet end 151 is used to communicate with one of the second outlet ends 312 of the intermediate channel 31. The third outlet end 152 is formed on the bottom surface 121 of the groove 120, and the dried blood paper 20 covers the third outlet end 152. In use, the operator can operate the operating switch 30 to put the dried blood spot quantitative dispensing device 100 into the connected state. At this time, the blood sample flowing in through the first inlet end 141 of the inlet channel 14 flows through the intermediate channel 31 and fills each capillary channel 15 under capillary action and extends to the third outlet end 152. At this time, the blood sample is quantified within the capillary channel 15. It can be understood that due to the tension of the blood sample itself, the blood sample will not flow out through the third outlet end 152 of the capillary channel 15. Then, the operator can operate the operating switch 30 to put the dried blood spot quantitative dispensing device 100 into the blocked state, disconnecting the blood source. The blood sample in the capillary channel 15 is printed onto the dried blood paper 20 through the third outlet end 152 under the suction of the dried blood paper 20, forming a circular dried blood spot. At this time, the blood sampling is completed. In other embodiments, a hydrolyzed gel (not shown) may be provided at the third outlet end 152 of the capillary channel 15. After the dried blood spot quantitative dispensing device 100 is in the connected state and the blood sample fills the capillary channel 15, the hydrolyzed gel can prevent the blood sample from flowing directly out from the third outlet end 152.

[0092] In some embodiments, the substrate 10 can be a transparent plastic plate, allowing the operator to observe whether the blood sample fills each capillary channel 15. When the blood sample fills each capillary channel 15, the operating switch 30 is activated to block the dried blood spot quantitative dispensing device 100. In other embodiments, the operating switch 30 can be activated to block the dried blood spot quantitative dispensing device 100 when the dried blood paper 20 just begins to contain the blood sample. Personal information such as the blood collection subject and collection time can be recorded on the dried blood paper 20. In some embodiments, the third outlet end 152 of each capillary channel 15 can be arranged along the same straight line and directly opposite the blood spot sampling point 21, with the volume of each capillary channel 15 corresponding to the size of the blood spot sampling point 21. Figure 7 As shown, at least one capillary channel 15 may be provided with a bent section, so as to make full use of the internal space of the substrate 10 while achieving the required volume.

[0093] In some embodiments, the inlet channel 14 may also be provided with a hollow needle portion (not shown), which may protrude from the first inlet end 141 of the inlet channel 14. The needle portion is used to pierce the blood collector (such as a negative pressure tube containing a blood sample), allowing the blood sample in the blood collector to flow into the inlet channel 14. In use, the dried blood spot quantitative dispensing device 100 can be placed vertically, i.e., the first inlet end 141 of the inlet channel 14 faces upward; then the blood collector is placed above the first inlet end 141, and the needle portion is used to pierce the blood collector, allowing the blood sample in the blood collector to flow into the inlet channel 14 under the action of gravity. Since the size of the fourth region 134 in the second direction Y is relatively large, the stability of the dried blood spot quantitative dispensing device 100 is improved when it is placed vertically. In some embodiments, the needle portion may be a hollow needle body made of metal, with a diameter of 0.1 mm to 2 mm.

[0094] like Figure 3 and Figure 4 As shown, in some embodiments, the dried blood spot quantitative dispensing device 100 may further include a sealing member 40, which may be disposed around the switch portion of the operating switch 30. When the dried blood spot quantitative dispensing device 100 is in the connected state, the sealing member 40 is offset from the first outlet end 142 of the inlet channel 14 and the third inlet end 151 of each capillary channel 15. When the dried blood spot quantitative dispensing device 100 is in the blocked state, the sealing member 40 is sealed to the first outlet end 142 of the inlet channel 14 and the third inlet end 151 of each capillary channel 15, thereby preventing blood samples in the inlet channel 14 from overflowing through the first outlet end 142 and preventing blood samples in each capillary channel 15 from overflowing through the third inlet end 151. In some embodiments, the sealing member 40 may be made of silicone.

[0095] The dried blood spot quantitative dispensing device 100 may further include a buffer member 50, which is disposed in the first direction X between the operating switch 30 and the bottom surface 111 of the slot 110. The buffer member 50 can buffer the operation switch 30 when it is rotated, preventing wear caused by frequent rotation. In addition, when the operating switch 30 is configured to be pressed along the first direction X to disconnect the second inlet end 311 from the inlet channel 14, the buffer member 50 can also be elastically compressed when the operating switch 30 is pressed, thereby providing space for the displacement of the operating switch 30 in the first direction X. In some embodiments, the second sealing member 40 may be made of silicone.

[0096] Please see Figures 9 to 12Another embodiment of this application provides a dried blood spot quantitative dispensing device 200. Unlike the dried blood spot quantitative dispensing device 100 described above, the operating switch 30 does not have an intermediate channel 31. In some embodiments, the operating switch 31 is slidably disposed within a slot 110 along a second direction Y, and the first outlet end 142 of the inlet channel 14 is connected to one end of the slot 110. In the second direction Y, the length of the operating switch 31 is less than the length of the slot 110. The operating switch 31 is used to switch the dried blood spot quantitative dispensing device 200 to an open state and an closed state when sliding.

[0097] The operating switch 30 includes a main body 32 and an operating part 33 connected to each other. The main body 32 is disposed within the substrate 10 and extends along the second direction Y. The operating part 33 protrudes from the first surface 11 of the substrate 10, and the operator can slide the operating switch 30 by operating the operating part 33. Further, in some embodiments, the main body 32 may include a first sealing part 32c, a second sealing part 32d disposed opposite to the first sealing part 32c in the second direction Y, and a connecting part 32e connecting the first sealing part 32c and the second sealing part 32d. In the third direction Y, the first sealing part 32c is closer to the first outlet end 142 than the second sealing part 32d. The operating part 33 is disposed on the connecting part 32e. The first sealing part 32c and the second sealing part 32d are both sealed and connected within the slot 110.

[0098] For example, the first sealing part 32c and the second sealing part 32d may be made of an elastic material (such as rubber). In the third direction Z, the original width of the first sealing part 32c (i.e., the width of the first sealing part 32c when not assembled in the slot 110) may be greater than the width of the slot 110, and the original width of the second sealing part 32d (i.e., the width of the second sealing part 32d when not assembled in the slot 110) may be greater than the width of the slot 110, so that the first sealing part 32c and the second sealing part 32d form an interference fit with the slot 110.

[0099] Therefore, as Figure 11 As shown, when the operating switch 30 is located at the end communicating with the first outlet end 142 of the slot 110, the first sealing part 32c can isolate the first outlet end 142 and the capillary channel 15 in the third direction X. At this time, the dried blood spot quantitative dispensing device 200 is in a blocked state, preventing blood samples from entering the multiple capillary channels 15 through the intermediate channel 31. Figure 12As shown, when the operating switch 30 slides to the other end of the slot 110 away from the first outlet end 14, a negative pressure is generated in the space between the end of the slot 110 connected to the first outlet end 142 and the first sealing part 32c. The first sealing part 32c does not isolate the first outlet end 142 and the capillary channel 15 in the third direction X. At this time, the dried blood spot quantitative dispensing device 200 is in a connected state, allowing the blood sample introduced through the inlet channel 14 to enter the slot 110 and multiple capillary channels 15 sequentially under the negative pressure within the slot 110. Due to the arrangement of the first sealing part 32c and the second sealing part 32d in the operating switch 30, the dried blood spot quantitative dispensing device 200 has better sealing and leak-proof effects when in a connected state, improving the situation where the blood sample introduced through the inlet channel 14 leaks out between the first outlet end 142 and the second inlet end 311 or between the second outlet end 312 and the third inlet end 151.

[0100] like Figure 9 and Figure 10 As shown, the slot 110 forms an opening 1100 on the first surface 11, and the operating part 33 of the operating switch 31 protrudes from the first surface 11 via the opening 1100. The opening 1100 includes a first opening segment 1100a, a second opening segment 1100b, and a third opening segment 1100c connected in sequence. The first opening segment 1100a and the third opening segment 1100c can both be arranged along the second direction Y, and the second opening segment 1100b can be arranged along the third direction Z. When the operating part 33 slides from the third opening segment 1100c to the first opening segment 1100a, the dried blood spot quantitative dispensing device 200 switches from a connected state to a blocked state.

[0101] In some embodiments, a blood collector 144 (such as a negative pressure tube for collecting blood samples) is detachably mounted on the side 13 of the substrate 10. When the blood collector 144 is mounted on the side 13, the blood sample in the blood collector 144 flows into the inlet channel 14 via the needle puncture part 143.

[0102] Please see Figures 13 to 15 Another embodiment of this application provides a dried blood spot quantitative dispensing device 300. The difference from the dried blood spot quantitative dispensing device 200 described above is that the blood collector 144 is integrally formed on the side 13. In this embodiment, the blood collector 144 can be mounted on another blood collection device (not shown). Under the action of negative pressure inside the blood collector 144, the blood sample in the blood collection device can first flow into the blood collector 144, and then by operating the operating switch 30, the blood sample in the blood collector 144 can be controlled to flow into the capillary channel 15 through the inlet channel 14.

[0103] Please see Figure 16This application also provides a method for quantitative dispensing of dried blood spots applied to the aforementioned quantitative dispensing device 100 (or quantitative dispensing devices 200, 300), comprising the following steps:

[0104] Step S1: Operate the operation switch 30 to switch the dried blood spot quantitative dispensing device 100 to the connected state, thereby connecting the inlet channel 14 to multiple capillary channels 15.

[0105] Step S2: A blood sample is introduced through the introduction channel 14, so that the blood sample fills each capillary channel 15 under capillary action and extends to the outlet end of the capillary channel 15.

[0106] Step S3: Operate the operation switch 30 to switch the dried blood spot quantitative dispensing device 100 to the blocking state, thereby disconnecting the inlet channel 14 and the multiple capillary channels 15.

[0107] In step S4, the blood sample in the capillary channel 15 is adsorbed by the suction of the dried blood paper 20, so that the blood sample flows through the outlet end of the capillary channel 15 to the dried blood paper 20 and forms a dried blood spot.

[0108] In the dried blood spot quantitative dispensing device 100 of this application, the capillary channel 15 is set to a predetermined volume according to actual needs. The operation switch 30 is operated to put the dried blood spot quantitative dispensing device 100 into a connected state. At this time, the blood sample flowing in through the first inlet end 141 of the inlet channel 14 fills each capillary channel 15 under the action of capillary and extends to the third outlet end 152. At this time, the blood is quantitatively collected in the capillary channel 15. Then, the operation switch 30 is operated to put the dried blood spot quantitative dispensing device 100 into a blocked state, disconnecting the blood source. The blood in the capillary channel 15 is printed onto the dried blood paper 20 through the third outlet end 152 under the suction of the dried blood paper 20, forming a circular dried blood spot, thereby completing the quantitative collection of the blood sample with high quantitative accuracy.

[0109] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this application and are not intended to limit it. Although this application has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of this application without departing from the spirit and scope of the technical solutions of this application.

Claims

1. A device for quantitative dispensing of dried blood spots, characterized in that, include: A substrate includes a first surface and a second surface disposed opposite to each other in a first direction. The substrate has a groove and a slot, each of which is independently formed on the first surface or the second surface. The substrate has an inlet channel and a plurality of capillary channels. The inlet channel is used to introduce a blood sample. Each capillary channel has a predetermined volume, and the outlet end of each capillary channel is located on the bottom surface of the groove. An operating switch is movably connected within the slot. The operating switch is used to switch the dried blood spot quantitative dispensing device to a connected state and a blocked state during operation. In the connected state, the inlet channel is connected to multiple capillary channels, allowing the blood sample introduced by the inlet channel to enter each capillary channel and fill each capillary channel under capillary action, extending to the outlet end of the capillary channel. In the blocked state, the inlet channel is disconnected from the multiple capillary channels. as well as A dried blood paper strip is disposed within the groove and covers the outlet end of each of the capillary channels. The dried blood paper strip is used to adsorb the blood sample in the capillary channel through the outlet end of each of the capillary channels and form a dried blood spot.

2. The dried blood spot quantitative dispensing device as described in claim 1, characterized in that, The operating switch is rotatably disposed within the slot. The operating switch has an intermediate channel. The operating switch is used to switch the dried blood spot quantitative dispensing device to the connected state and the blocked state when rotated. In the connected state, the intermediate channel is connected to the inlet channel and the multiple capillary channels respectively. In the blocked state, the intermediate channel is disconnected from the inlet channel and the multiple capillary channels respectively.

3. The dried blood spot quantitative dispensing device as described in claim 1, characterized in that, The operating switch is slidably disposed in the slot along the first direction. The operating switch has an intermediate channel. The operating switch is used to switch the dried blood spot quantitative dispensing device to the connected state and the blocked state when sliding along the first direction. In the connected state, the intermediate channel is connected to the inlet channel and the multiple capillary channels respectively. In the blocked state, the intermediate channel is disconnected from the inlet channel and the multiple capillary channels respectively.

4. The dried blood spot quantitative dispensing device as described in claim 2 or 3, characterized in that, The dried blood spot quantitative dispensing device also includes a sealing element, which is arranged around the operating switch. When the dried blood spot quantitative dispensing device is in the connected state, the sealing element is offset from the outlet end of the inlet channel and the inlet end of each capillary channel. When the dried blood spot quantitative dispensing device is in the blocked state, the sealing element is sealed to the outlet end of the inlet channel and the inlet end of each capillary channel.

5. The dried blood spot quantitative dispensing device as described in claim 2 or 3, characterized in that, The dried blood spot quantitative dispensing device further includes a buffer element, which is disposed in the first direction between the operating switch and the bottom surface of the slot.

6. The dried blood spot quantitative dispensing device as described in claim 1, characterized in that, The operating switch is slidably disposed in the slot along a second direction perpendicular to the first direction. The outlet end of the inlet channel is connected to one end of the slot. The operating switch is used to switch the dried blood spot quantitative dispensing device to the connected state and the blocked state when sliding along the second direction. The connected state is that a negative pressure is generated in the space between the end of the slot and the operating switch, and the inlet channel is connected to a plurality of capillary channels through the space. The blocked state is that the operating switch isolates the end and the plurality of capillary channels, thereby disconnecting the inlet channel from the plurality of capillary channels. The operating switch includes a main body and an operating part. The main body is disposed in the slot. The main body includes a first sealing part and a second sealing part disposed opposite to each other in the second direction, and a connecting part connected between the first sealing part and the second sealing part. The operating part is connected to the connecting part and protrudes from the slot. The first sealing part and the second sealing part are both sealed and connected in the slot.

7. The dried blood spot quantitative dispensing device as described in claim 6, characterized in that, The substrate also includes a side surface connected between the first surface and the second surface, the inlet end of the introduction channel is located on the side surface, and the dried blood spot quantitative dispensing device also includes a blood collector detachably mounted on the side surface and a hollow needle part located in the introduction channel, the needle part protruding from the inlet end of the introduction channel and extending into the blood collector.

8. The dried blood spot quantitative dispensing device as described in claim 6, characterized in that, The substrate also includes a side surface connected between the first surface and the second surface, the inlet end of the introduction channel is located on the side surface, the dried blood spot quantitative dispensing device also includes a blood collector integrally installed on the side surface, a negative pressure is formed inside the blood collector, and the inlet end of the introduction channel is connected to the blood collector.

9. The dried blood spot quantitative dispensing device as described in claim 1, characterized in that, The dried blood paper has several blood spot sampling points. The outlet ends of the multiple capillary channels are arranged along the same straight line and are directly opposite the blood spot sampling points. The volume of each capillary channel corresponds to the size of the blood spot sampling point. At least one capillary channel is provided with a bent section.

10. The dried blood spot quantitative dispensing device as described in claim 1, characterized in that, The substrate is made of transparent plastic.

11. A method for quantitatively dispensing dried blood spots using a quantitative dispensing apparatus as described in any one of claims 1 to 10, characterized in that, include: Operate the operating switch to switch the dried blood spot quantitative dispensing device to the connected state, thereby connecting the inlet channel to the multiple capillary channels; A blood sample is introduced through the inlet channel, so that the blood sample fills each capillary channel under capillary action and extends to the outlet end of the capillary channel; Operate the operating switch to switch the dried blood spot quantitative dispensing device to the blocking state, thereby disconnecting the inlet channel and the multiple capillary channels; as well as The blood sample in the capillary channel is adsorbed by the suction of the dried blood paper, so that the blood sample flows through the outlet end of the capillary channel to the dried blood paper and forms a dried blood spot.

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