Method for improving transplant survival rate of embelia laevigata wall

Abstract

The present application relates to a method for improving transplant survival rate of kadsura coccinea, belonging to the technical field of horticultural plant cultivation. After the peanut is germinated, the peanut is planted together with the kadsura coccinea during the operation of lying planting, and the AM mycorrhizal fertilizer is added to the substrate during the operation of soil substrate preparation, so that more symbiotic bacteria are produced under the joint action of the peanut and the mycorrhizal fertilizer in the substrate, and the symbiotic relationship is formed with the root system of the kadsura coccinea, the nutrient absorption of the kadsura coccinea plant is promoted, and the survival rate of the kadsura coccinea is improved. During the operation of root incision, more wound surfaces are formed on the root of the kadsura coccinea plant, so that more callus tissues are formed on the root, thereby more root systems are differentiated. During the operation of air-drying of roots, the air-drying of roots of the kadsura coccinea plant can prevent infection of wound flow, and create moderate drought stress, so that the root system is differentiated after rapid water absorption after transplanting. During the operation of root grafting, more rooting sites are regenerated at the root grafting position of the kadsura coccinea, and more root systems are differentiated. During the operation of lying planting, the transport water potential of the kadsura coccinea plant after root breaking is reduced, and the survival rate is improved.

Description

Technical Field

[0001] This invention belongs to the field of horticultural and garden plant cultivation technology, specifically, it relates to a method for improving the survival rate of transplanted *Sedum morganianum*. Background Technology

[0002] *Embelia laeta* (L.) Mez is a climbing shrub or vine belonging to the genus *Embelia* in the family Myrsinaceae. It is mainly distributed in sparse forests or thickets on mountain slopes in southern my country and the Indochina Peninsula. It has a prominent taproot that penetrates deeply into the ground, with few lateral and fibrous roots; there are virtually no lateral roots in the top 30-50 cm of soil. The root periderm and epidermis are thick and fleshy, exhibiting significant lignification. Due to its brightly colored fruits, *Embelia laeta* is a high-quality ornamental horticultural plant, highly favored by bonsai enthusiasts. However, *Embelia laeta* is known for its "death upon transplanting" phenomenon; after transplanting, its roots struggle to form callus tissue or the callus tissue rarely differentiates into roots, leading to the stem dying after consuming its own nutrients to produce water buds (false survival), resulting in an extremely low survival rate. Summary of the Invention

[0003] The purpose of this invention is to provide a method for improving the survival rate of transplanted *Sedum morganianum*, which can effectively increase the survival rate of transplanted *Sedum morganianum*.

[0004] The objective of this invention can be achieved through the following technical solutions:

[0005] A method for improving the survival rate of transplanted *Sedum morganianum* includes the following steps:

[0006] Peanut germination: Germinate peanut seeds with a 1000-1500 times diluted rooting powder solution for 12-16 hours, then remove and air dry;

[0007] Soil substrate preparation: Add AM mycorrhizal fertilizer to the soil substrate, then add water until the substrate moisture content is 70-90%, and spread it out in a 15-20cm layer.

[0008] Harvesting: Harvest the sour vine in March and April, and cut off the healthy parts of the branches to keep them moist; trim the branches and roots of the harvested sour vine, smooth the cut ends of the stems and roots, and apply a healing agent to the cut ends of the stems;

[0009] Root carving: Make 3 to 5 cuts at different positions along the vertical direction of the root to form root incisions, with each two adjacent cuts spaced 5 to 8 cm apart;

[0010] Root drying: After cutting the roots, wrap the stems of the *Symplocos edulis* with film and let the roots dry in a shaded and ventilated place for 24 to 36 hours;

[0011] Root grafting: For the roots of the sorrel vine, use healthy branches cut off during harvesting to perform T-shaped root grafting at 3 to 5 different locations on the root.

[0012] Horizontal planting: After root grafting, the sorrel is dipped in rooting powder and then planted at an angle in the soil substrate until the roots of the sorrel are completely buried. After germination, peanuts are sown directly above the roots of the sorrel in the soil substrate and planted together with the sorrel in the substrate for cultivation.

[0013] Maintenance: After planting, cover the soil substrate with a transparent film, spray with rooting powder, and water with mycorrhizal fertilizer. Remove the transparent film from the soil substrate after 46-60 days. Prune regularly, remove the peanuts after they mature, and backfill the substrate.

[0014] Straighten and add soil: After 140 to 160 days, prune the stems and leaves, straighten the main root and put on a root control device, fill in the soil, water thoroughly and continue cultivation;

[0015] Transplanting: Remove the root control device in February or March of the following year and transplant the Sour Vine.

[0016] Preferably, in the preparation of the soil matrix, the soil matrix is ​​weathered rock and soil of river sand and granite with a mass ratio of 1:0.8 to 1.2.

[0017] Preferably, in the root carving, the root carving specifically involves: making a first cut perpendicular to the root of the vine to the xylem, and then making another cut 1.5 to 3.5 mm above the first cut at an angle of 45 to 60 degrees to the xylem and intersecting with the first cut to form a 45 to 60 degree oblique cut.

[0018] Preferably, in root grafting, the root grafting specifically involves taking healthy branches cut during harvesting, soaking them in a 1000-1500 times diluted rooting powder solution for 1-2 hours, and performing 3-5 root grafts at different locations on the other side of the cut root, with each adjacent pair of grafts spaced 5-8 cm apart.

[0019] Preferably, in the horizontal planting method, the planting specifically involves dipping the root of the grafted *Sedum morganianum* in a 300-500 times rooting powder solution for 5-10 seconds, then removing it and planting it at a 0-30° angle into the prepared soil substrate, so that the root cutting and root grafting parts of *Sedum morganianum* are completely buried in the substrate, with the stem exposed.

[0020] Preferably, in the horizontal planting method, the sprouted peanuts are sown directly above the roots of the sorrel in the soil substrate, specifically by sowing the sprouted peanuts evenly at intervals of 5-8 cm directly above the roots of the sorrel.

[0021] Preferably, during maintenance, the spraying of rooting powder specifically involves spraying a rooting powder solution diluted 1500 to 2000 times, with a frequency of every 5 to 7 days; the irrigation of mycorrhizal fertilizer specifically involves irrigating with an AM mycorrhizal fertilizer solution diluted 300 to 800 times, with a frequency of every 20 to 30 days.

[0022] Preferably, during maintenance, the regular pruning specifically involves cutting the branches of the sorrel growing out of the soil short every 30 to 45 days until 2 to 3 cm of the substrate is exposed, digging down along the branches to the root joint, cutting off the binding tape, scraping the bottom of the branch at the root joint with a knife until the xylem is exposed, and then backfilling.

[0023] Preferably, during maintenance, after the peanuts mature and are removed, all branches growing from the root joint of the sorrel are pruned, all hidden buds are dug out, and then the substrate is backfilled.

[0024] Preferably, in the process of straightening and adding soil, the filling soil is yellow soil.

[0025] The method provided by this invention has the following beneficial effects:

[0026] First, after the peanuts are sprouted, they are planted together with the sorrel in the substrate during the horizontal planting process. During the soil substrate preparation, AM mycorrhizal fertilizer is added to the substrate so that the peanuts and mycorrhizal fertilizer work together in the substrate to produce more symbiotic bacteria and form a symbiotic relationship with the roots of the sorrel, which promotes the absorption of nutrients by the sorrel plants and improves the survival rate of the sorrel.

[0027] Secondly, during the root cutting operation, the root cutting of the *Solanum tuberosum* plant creates more wound surfaces, which promotes the formation of more callus tissue in the roots, thereby differentiating more roots.

[0028] Third, during the root drying process, the stem of the *Sedum aizoon* plant is kept moist while the roots are dried to prevent sap flow infection and create a moderately high drought stress, which allows it to quickly absorb water after transplanting, produce callus tissue, and differentiate into roots.

[0029] Fourth, during root grafting, the grafted site of *Syzygium sambac* can generate more potential rooting sites and differentiate more roots;

[0030] Fifth, during the horizontal planting operation, the water transport potential of the *Sedum lineare* plant after root pruning is reduced, thereby increasing the survival rate. Detailed Implementation

[0031] The present invention will be further described in detail below with reference to specific embodiments.

[0032] In April 2023, healthy wild *Solanum tuberosum* plants of similar size and growth were collected in Maoming City, Guangdong Province (to test the survival rate of the taproot, only one taproot was left during collection, and the lateral roots on the taproot were removed), and brought back to Dongguan City, Guangdong Province for planting trials. The AM mycorrhizal fertilizer was purchased from Hebi Ruipu Huizhong Biotechnology Co., Ltd., and the ABT rooting powder was purchased from Beijing Aibiti Biotechnology Co., Ltd., details of which will not be repeated below.

[0033] Example 1: Transplanting of *Solanum tuberosum*, the following operations were performed in sequence:

[0034] (1) Peanut sprouting

[0035] Select plump, healthy, and disease-free peanut seeds from the legume family. Soak them in a 1000-fold dilution of ABT rooting powder solution (m water:m rooting powder = 1000:1) to promote germination. After 12 hours, remove them and let them dry.

[0036] (2) Soil substrate preparation

[0037] Select well-ventilated, clean river sand and weathered granite soil, mix them in a 1:1 mass ratio, and sun-dry them thoroughly. Then add 0.1% AM mycorrhizal fertilizer by mass, add clean water, stir well to allow it to reabsorb water to a moisture content of 70%, and spread it in the planting bed, maintaining a thickness of 15-20cm.

[0038] (3) Carving roots

[0039] Dig up 9 vines of the sour vine, prune the branches and roots, retaining 30-40cm of stems and 20-30cm of roots underground. Smooth the cut ends of the stems and roots, and apply a healing agent to the cut ends of the stems.

[0040] Make 3 to 5 cuts at different positions along the vertical direction of the root to form root incisions. Each pair of adjacent cuts should be 5 to 8 cm apart. The specific method of root carving is as follows: the first cut is perpendicular to the root and cuts to the wood. The second cut is made 1.5 to 3.5 mm above the first cut and cuts at a 45° angle along the direction of the first cut to the wood and intersects with the first cut to form a 45° oblique cut.

[0041] In addition, when harvesting, cut off the healthy parts of the branches and bring them back to keep them moist.

[0042] (4) Drying the roots

[0043] Wrap the cut roots of the *Sedum aizoon* stems in film and place them in a shaded and ventilated place for 24 hours to allow them to dry, preventing sap flow infection and creating a moderate drought stress.

[0044] (5) Root grafting

[0045] After the roots have dried, use healthy branches cut during harvesting to perform "T-shaped grafting" on the roots of the *Solanum tuberosum*. Take the branches brought back during harvesting, soak them in a 1000-fold dilution of ABT rooting powder solution (m water:m rooting powder = 1000:1) for 1 hour, and perform 3-5 grafts at different locations every 5-8 cm on the root (adjacent to the notched root locations), and tighten the grafting site with a tie (do not wrap with film).

[0046] (6) Lying down

[0047] Dip the grafted roots of *Sedum aizoon* in a 300-fold dilution of ABT rooting powder solution (m water:m rooting powder = 300:1) for 5 seconds, then remove them and plant them at a 30° angle in a planting bed of mixed river sand and weathered rock substrate containing AM mycorrhizal fertilizer, ensuring the substrate completely covers the roots. Sow the germinated peanuts evenly at intervals of 5-8 cm directly above the roots of *Sedum aizoon*, and level the planting bed.

[0048] (7) Maintenance

[0049] After planting, cover the planting bed with a transparent film to retain moisture.

[0050] Every 5 days, spray with a 1500-fold dilution of ABT rooting powder solution (m water:m rooting powder = 1500:1) depending on soil moisture (maintain 80% humidity); every 20 days, irrigate with a 300-fold dilution of AM mycorrhizal fertilizer solution. Open the transparent film covering the planting bed after 45 days.

[0051] Every 30 days, cut the branches growing from the substrate to 2-3 cm, dig down the branches to the root joint, cut off the binding tape, scrape the bottom of the branch at the root joint with a knife until the xylem is exposed, and then backfill.

[0052] In 120-135 days, the peanuts will mature. Remove the peanuts, prune all branches growing from the root graft, and dig out all hidden buds (be careful to avoid breaking the graft). Backfill with substrate and continue cultivation.

[0053] Survival rate and growth status record: After 6 months, the survival rate of transplanted *Sedum morganianum* in Example 1 was 77.78%. During the growth process, all plants sprouted new buds, with the stems sprouting first and the root grafting points sprouting later. The above-ground branches grew faster than the underground branches. In the later stages, a small number of plants gradually withered and died from top to bottom. The cuts, notches, and root grafting points of the dead plants had callus tissue and new roots. The surviving plants had the most abundant and well-developed root systems, especially at the root grafting points, and the plants grew well.

[0054] Example 2: Transplanting of *Solanum tuberosum*, the following operations were performed in sequence:

[0055] (1) Peanut sprouting

[0056] Select plump, healthy, and disease-free peanut seeds from the legume family. Soak them in a 1250-fold dilution of ABT rooting powder solution (m water:m rooting powder = 1250:1) to promote germination. After 14 hours, remove them and let them air dry.

[0057] (2) Soil substrate preparation

[0058] Select well-ventilated, clean river sand and weathered granite soil, mix them at a mass ratio of 1:0.8, and sun-dry them thoroughly. Then add 0.3% AM mycorrhizal fertilizer, add clean water, stir well to allow it to reabsorb water to 80% moisture content, and spread it in the planting bed, keeping the thickness at 15-20cm.

[0059] (3) Carving roots

[0060] Harvest the sour vine in March and April, prune the branches and roots, keeping 30-40cm of stem and 20-30cm of roots underground. Smooth the cut ends of the stem and roots, and apply a healing agent to the cut ends of the stem.

[0061] Make 3 to 5 cuts at different positions along the vertical direction of the root to form root incisions. Each pair of adjacent cuts should be 5 to 8 cm apart. The specific method of making the cuts is as follows: the first cut is made vertically to the root and down to the wood. The second cut is made 1.5 to 3.5 mm above the first cut and at a 50° angle along the direction of the first cut down to the wood and intersects with the first cut to form a 50° oblique cut.

[0062] In addition, when harvesting, cut off the healthy parts of the branches and bring them back to keep them moist.

[0063] (4) Drying the roots

[0064] Wrap the cut roots of the *Sedum aizoon* stems in film and place them in a shaded and ventilated place for 30 hours to allow them to dry, preventing sap flow infection and creating a moderate drought stress.

[0065] (5) Root grafting

[0066] After the roots have dried, use healthy branches cut during harvesting to perform "T-shaped grafting" on the roots of the *Solanum tuberosum*. Take the branches brought back during harvesting, soak them in a 1250-fold dilution of ABT rooting powder solution (m water:m rooting powder = 1250:1) for 1.5 hours, and perform 3-5 graftings at different locations every 5-8 cm on the root (adjacent to the notched root locations), and tighten the grafting site with a tie (without wrapping with film).

[0067] (6) Lying down

[0068] Dip the grafted roots of *Sedum aizoon* in a 400-fold rooting powder solution (m water:m rooting powder = 400:1) for 8 seconds, then remove them and plant them at a 15° angle in a planting bed of mixed river sand and weathered rock substrate containing AM mycorrhizal fertilizer, ensuring the substrate completely covers the roots. Sow the germinated peanuts evenly at intervals of 5-8 cm directly above the *Sedum aizoon* roots and level the planting bed.

[0069] (7) Maintenance

[0070] After planting, cover the planting bed with a transparent film to retain moisture.

[0071] Spray with a 1800-fold dilution of ABT rooting powder solution (m water:m rooting powder = 1800:1) every 6 days, depending on soil moisture (maintain 80% humidity); water with a 500-fold dilution of AM mycorrhizal fertilizer solution every 25 days. Open the transparent film covering the planting bed after 50 days.

[0072] Every 40 days, prune the branches growing from the substrate to 2-3 cm, dig down the branches to the root joint, cut off the binding tape, scrape the bottom of the branch at the root joint with a knife until the xylem is exposed, and then backfill.

[0073] In 120-135 days, the peanuts will mature. Remove the peanuts, prune all branches growing from the root graft, and dig out all hidden buds (be careful to avoid breaking the graft). Backfill with substrate and continue cultivation.

[0074] (8) straighten and add soil

[0075] After 140-160 days, prune the stems and leaves appropriately, straighten the main root (no need to lift the whole plant, protect the lateral roots growing from the root notch and root joint), put on the root control device, and fill it with clean, low-porosity yellow soil until all roots are completely buried. Water thoroughly and continue cultivation.

[0076] (9) Transplanting

[0077] In February or March of the following year, prune the branches and leaves, remove the root control device, and do not prune the root system (to protect the core soil). Pot or transplant to other places.

[0078] Example 3: Transplanting of *Solanum tuberosum*, the following operations were performed in sequence:

[0079] (1) Peanut sprouting

[0080] Select plump, healthy, and disease-free peanut seeds from the legume family. Soak them in a 1500-fold dilution of ABT rooting powder solution (m water:m rooting powder = 1500:1) to promote germination. After 16 hours, remove them and let them air dry.

[0081] (2) Soil substrate preparation

[0082] Select well-ventilated, clean river sand and weathered granite soil, mix them in a mass ratio of 1:1.2, and sun-dry them thoroughly. Then add 0.5% AM mycorrhizal fertilizer, clean water, and stir well to allow it to reabsorb water to a moisture content of 90%. Spread it in the planting bed, keeping the thickness at 15-20cm.

[0083] (3) Carving roots

[0084] Harvest the sour vine in March and April, prune the branches and roots, keeping 30-40cm of stem and 20-30cm of roots underground. Smooth the cut ends of the stem and roots, and apply a healing agent to the cut ends of the stem.

[0085] Make 3 to 5 cuts along the vertical arrangement of the roots to form root incisions. Each pair of adjacent cuts should be 5 to 8 cm apart. The specific method for making the cuts is as follows: the first cut is made vertically to the woody part of the root. The second cut is made 1.5 to 3.5 mm above the first cut, at a 60° angle along the direction of the first cut, to the woody part and intersecting with the first cut to form a 60° oblique cut.

[0086] In addition, when harvesting, cut off the healthy parts of the branches and bring them back to keep them moist.

[0087] (4) Drying the roots

[0088] Wrap the cut roots of the *Sedum aizoon* stems in film and place them in a shaded and ventilated place for 36 hours to allow them to dry, preventing sap flow infection and creating a moderate drought stress.

[0089] (5) Root grafting

[0090] After the roots have dried, use healthy branches cut during harvesting to perform "T-shaped grafting" on the roots of the *Solanum tuberosum*. Take the branches brought back during harvesting, soak them in a 1500-fold dilution of ABT rooting powder solution (m water:m rooting powder = 1500:1) for 2 hours, and perform 3-5 grafts at different locations every 5-8 cm on the root (adjacent to the notched root locations), and tighten the grafting site with a tie (without wrapping with film).

[0091] (6) Lying down

[0092] Dip the grafted roots of *Sedum aizoon* in a 500-fold rooting powder solution (m water:m rooting powder = 300-500:1) for 10 seconds, then remove them and plant them at a 0° angle in a planting bed of mixed river sand and weathered rock substrate containing AM mycorrhizal fertilizer, ensuring the substrate completely covers the roots. Sow the germinated peanuts evenly at intervals of 5-8 cm directly above the *Sedum aizoon* roots, and level the planting bed.

[0093] (7) Maintenance

[0094] After planting, cover the planting bed with a transparent film to retain moisture.

[0095] Every 7 days, spray with a 2000-fold dilution of ABT rooting powder solution (m water:m rooting powder = 2000:1) depending on soil moisture (maintain 80% humidity); every 30 days, irrigate with an 800-fold dilution of AM mycorrhizal fertilizer solution. Open the transparent film covering the planting bed after 60 days.

[0096] Every 45 days, prune the branches growing from the substrate to 2-3 cm, dig down the branches to the root joint, cut off the binding tape, scrape the bottom of the branch at the root joint with a knife until the xylem is exposed, and then backfill.

[0097] In 120-135 days, the peanuts will mature. Remove the peanuts, prune all branches growing from the root graft, and dig out all hidden buds (be careful to avoid breaking the graft). Backfill with substrate and continue cultivation.

[0098] (8) straighten and add soil

[0099] After 140-160 days, prune the stems and leaves appropriately, straighten the main root (no need to lift the whole plant, protect the lateral roots growing from the root notch and root joint), put on the root control device, and fill it with clean, low-porosity yellow soil until all roots are completely buried. Water thoroughly and continue cultivation.

[0100] (9) Transplanting

[0101] In February or March of the following year, prune the branches and leaves, remove the root control device, and do not prune the root system (to protect the core soil). Pot or transplant to other places.

[0102] Based on Example 1, a blank control group and a comparative control group were set up (see Table 1), with 9 plants in each group. The survival rate and growth of each group were recorded, and the results are shown in Table 1.

[0103] Table 1

[0104]

[0105] in:

[0106] Blank group: This is a blank treatment. In the early stage, most plants sprout new buds, and the branches are 10-30cm in length. After 40-60 days, most branches and leaves gradually wither and die. There is no callus tissue at the cut of the main root of the dead plants.

[0107] Comparative Example 1: In the early stage, most plants sprouted new buds, with branches ranging from 10 to 30 cm in length; after 40 to 60 days, most branches and leaves gradually withered and died. The dead plants had a small amount of callus tissue at the cut of the main root, and the surviving plants grew slightly better than the control group.

[0108] Comparative Example 2: In the early stage, most plants sprouted new buds, with the stems sprouting first and the root grafting sites sprouting later. The above-ground branches grew faster than the underground branches. In the later stage (50-60 days), some plants gradually withered and died from top to bottom. The main root cuts, root notches, and root grafting sites of the dead plants had no callus tissue. The surviving plants had few roots.

[0109] Comparative Example 3: All plants sprouted new buds, with the stems sprouting first and the root grafting sites sprouting later. The above-ground branches grew faster than the underground branches. Later, some plants gradually withered and died from top to bottom. The dead plants had a small amount of callus tissue at the cuts, notches, and root grafting sites of the main root. The surviving plants had slightly weaker root systems than Comparative Example 2 and their overall growth was slightly better than that of Comparative Example 2.

[0110] Comparative Example 4: All plants sprouted new buds, with the stems sprouting first and the root grafting sites sprouting later. The above-ground branches grew faster than the underground branches. Later, some plants gradually withered and died from top to bottom. The dead plants had callus tissue at the cuts, notches, and root grafting sites of the main root. The overall growth of the surviving plants was similar to that of Comparative Example 3.

[0111] Comparative Example 5: All plants sprouted new buds, with branch lengths ranging from 10 to 40 cm. The stems sprouted first, followed by the root grafts. The above-ground branches grew faster than the underground branches. In the later stages (50-60 days), most of the plants' branches and leaves gradually withered and died from top to bottom. The dead plants had callus tissue at the cut ends, notched roots, and root grafts of the main root.

[0112] Comparative Example 6: All plants sprouted new buds, with the stems sprouting first and the root grafting sites sprouting later. The above-ground branches grew faster than the underground branches. Later, some plants gradually withered and died from top to bottom. The cuts on the main roots and the root grafting sites of the dead plants had callus tissue and a small number of new roots.

[0113] Comparative Example 7: All plants sprouted new buds, with the stems sprouting first and the root grafts sprouting later. The above-ground branches grew faster than the underground branches. Later, some plants gradually withered and died from top to bottom. The cuts and notches on the main roots of the dead plants had a small amount of callus tissue and a small number of new roots. The overall growth of the surviving plants was similar to that of Comparative Examples 3, 4, and 6.

[0114] The above description is merely an embodiment of the present invention and does not limit the scope of patent protection. Any non-substantial changes or substitutions made by those skilled in the art based on the present invention will still fall within the scope of patent protection.

Claims

1. A method for improving the survival rate of transplanted *Sedum morganianum*, characterized in that, Includes the following steps: Peanut germination: Germinate peanut seeds with a 1000-1500 times diluted rooting powder solution for 12-16 hours, then remove and air dry; Soil substrate preparation: Add AM mycorrhizal fertilizer to the soil substrate, then add water until the substrate moisture content is 70-90%, and spread it out in a 15-20cm layer. Harvesting: Harvest the sour vine in March and April, and cut off the healthy parts of the branches to keep them moist; trim the branches and roots of the harvested sour vine, smooth the cut ends of the stems and roots, and apply a healing agent to the cut ends of the stems; Root carving: Make 3 to 5 cuts at different positions along the vertical direction of the root to form root incisions, with each two adjacent cuts spaced 5 to 8 cm apart; Root drying: After cutting the roots, wrap the stems of the sour vine with film and let the roots dry in a shaded and ventilated place for 24-36 hours; Root grafting: For the roots of the sorrel vine, use healthy branches cut off during harvesting to perform T-shaped root grafting at 3-5 different locations on the root. Horizontal planting: After root grafting, the sorrel is dipped in rooting powder solution and then planted at an angle in the soil substrate until the roots of the sorrel are completely buried. After germination, peanuts are sown directly above the roots of the sorrel in the soil substrate and planted together with the sorrel in the substrate for cultivation. Maintenance: After planting, cover the soil substrate with a transparent film, spray with rooting powder, and water with mycorrhizal fertilizer. Remove the transparent film from the soil substrate after 46-60 days. Prune regularly, remove the peanuts after they mature, and backfill the substrate. Straighten and add soil: After 140-160 days, prune the stems and leaves, straighten the main root and put on a root control device, fill in the soil, water thoroughly and continue cultivation; Transplanting: Remove the root control device in February or March of the following year and transplant the Sour Vine.

2. The method according to claim 1, characterized in that, In the soil substrate preparation, the soil substrate is a mixture of river sand and weathered granite in a mass ratio of 1:0.8~1.

2.

3. The method according to claim 1, characterized in that, In the root carving process, the root carving specifically involves: making a first cut perpendicular to the root of the vine to the woody part, and then making another cut 1.5 to 3.5 mm above the first cut at an angle of 45 to 60 degrees to the woody part and intersecting with the first cut to form a 45 to 60 degree oblique cut.

4. The method according to claim 1, characterized in that, In root grafting, the root grafting specifically involves taking healthy branches that have been cut during harvesting, soaking them in a 1000-1500 times diluted rooting powder solution for 1-2 hours, and then grafting them at 3-5 different locations on the other side of the cut root, with each adjacent graft spaced 5-8 cm apart.

5. The method according to claim 1, characterized in that, In the horizontal planting process, the planting is specifically carried out by dipping the root grafted *Sedum morganianum* root in a 300-500 times rooting powder solution for 5-10 seconds, then removing it and planting it at a 0-30° angle into the prepared soil substrate, so that the root cut and root grafting part of *Sedum morganianum* are completely buried in the substrate, with the stem part exposed.

6. The method according to claim 1, characterized in that, In the horizontal planting process, the sprouted peanuts are sown directly above the roots of the sorrel in the soil substrate. Specifically, the sprouted peanuts are sown evenly at intervals of 5-8 cm directly above the roots of the sorrel.

7. The method according to claim 1, characterized in that, During maintenance, the spraying of rooting powder specifically refers to spraying a rooting powder solution diluted 1500 to 2000 times, with a frequency of every 5 to 7 days; the watering of mycorrhizal fertilizer specifically refers to watering with an AM mycorrhizal fertilizer solution diluted 300 to 800 times, with a frequency of every 20 to 30 days.

8. The method according to claim 1, characterized in that, During maintenance, the regular pruning specifically involves cutting the branches of the sorrel that grow out of the soil short every 30 to 45 days, exposing 2 to 3 cm of the substrate, digging down along the branches to the root joint, cutting off the binding tape, scraping the bottom of the branch at the root joint with a knife until the xylem is exposed, and then backfilling.

9. The method according to claim 1, characterized in that, During maintenance, after the peanuts mature and are removed, all branches growing from the root joint of the sorrel should be pruned, all hidden buds should be dug out, and then the substrate should be backfilled.

10. The method according to claim 1, characterized in that, In the process of straightening and adding soil, the filling soil is yellow soil.

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