Agricultural composition for controlling pectinophora gossypii

Abstract

The present application belongs to the technical field of agricultural compositions, and relates to an agricultural composition for preventing and treating populus euphratica wood lice. The present application provides an agricultural composition for preventing and treating populus euphratica wood lice, wherein the effective components are composed of fluopyram and echinacoside, and the mass ratio of fluopyram to echinacoside is 1:1-37.5. It is found through insecticidal effect verification that echinacoside has a synergistic effect on the chemical insecticidal component fluopyram, and the combination of the two can effectively reduce the effective component dosage of chemical pesticides, fully exert the efficacy of fluopyram, as much as possible reduce the probability of populus euphratica wood lice developing drug resistance, reduce environmental pollution, and improve the effect of fluopyram.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of agricultural compositions, and relates to an agricultural composition for preventing and treating Pityus pyri. BACKGROUND

[0002] The eggs of Pityus pyri are mainly produced on leaves in a tender state at that time. After the eggs hatch, the first instar nymphs stop moving after crawling to the tender leaves at the top of the branches, start feeding, and stimulate the host tissue to be concave and hyperplastic. The hyperplastic part gradually wraps the body in the leaf to form a gall, one gall one worm. When the number of insect population is large, the front and back surfaces of the leaves are covered with galls, causing the leaves to wither and fall off, which seriously affects the growth of Pityus pyri and restricts the development of the Pityus pyri industry.

[0003] Fluazaindolizine is a butenolide compound, a nicotinic acetylcholine receptor agonist, and is classified into the Group 4D subgroup of nicotinic acetylcholine receptor modulators by the International Pesticide Resistance Action Committee (IRAC). Fluazaindolizine can selectively act on the central nervous system of insects, bind to receptor proteins, and then activate the receptors to produce biological reactions, so that the nerve cells are in an excited state. However, fluazaindolizine cannot be inactivated by binding to acetylcholine esterase, so the receptors remain open, leading to the collapse of the insect nervous system. Fluazaindolizine contains a special pharmacophore butenolide, has good systemic translocation, and can be rapidly translocated to various parts of the plant after foliar spraying or seed treatment. It can effectively control pests that feed on the back or hidden parts of leaves, and can reduce the transmission of pathogens that depend on insect vectors by inhibiting the feeding of insects. Fluazaindolizine, as a broad-spectrum insecticide, can also be used for the prevention and control of Pityus pyri.

[0004] However, during the growth of Pityus pyri, long-term application of a single chemical pesticide can easily cause pests to develop resistance. With the accumulation of pest resistance, the control effect of chemical pesticides gradually decreases, and even fails. However, reasonable compounding of effective ingredients is one of the effective and convenient means for developing pesticides and preventing and controlling resistant pests.

[0005] CN118077707A discloses a pesticide composition for preventing and controlling Dialeuroides oleivorus, which comprises fluoximide, bifenthrin and fluazaindolizine as effective ingredients. The mass ratio of fluoximide to bifenthrin is 1-40:25-1. The pesticide composition has a synergistic effect on Dialeuroides oleivorus.

[0006] CN111436441A discloses a pesticide composition for preventing and controlling Dialeuroides oleivorus, which comprises fluazaindolizine as a first active ingredient and amitraz as a second active ingredient. The mass ratio of fluazaindolizine to amitraz is 1:2-1:3.5. The pesticide composition has a significant effect on preventing and controlling Dialeuroides oleivorus by utilizing the complementary effects of fluazaindolizine and amitraz in terms of efficacy.

[0007] The above-mentioned patents all use known chemical pesticide components to compound with flupyradifurone to improve efficacy for woodlouse control. Long-term use still has environmental pollution problems and potential resistance problems. Finding other environmentally friendly and less likely to develop resistance components to compound with flupyradifurone for poplar woodlouse control has become a problem to be solved at present.

[0008] Echinacoside (ECH) is a phenylethanoid glycoside compound, which has pharmacological effects of neuroprotective effect, myocardial protective effect, liver protective effect, anti-tumor, antioxidant and anti-aging, immune regulation, hypoglycemic and hypolipidemic, promoting reproduction and bone formation, protecting pulmonary vessels, and preventing atherosclerosis. Echinacoside is environmentally friendly and has no pest resistance reports. There is also no literature report on the application of echinacoside and pesticide components to poplar woodlouse control. The ordinary skilled in the art cannot know whether echinacoside can be compounded with existing pesticide components to produce synergistic or antagonistic effects without experiments. The applicant found through experimental research that the insecticidal efficiency of poplar woodlouse was significantly improved after echinacoside was mixed with flupyradifurone at a certain proportion. In addition, the use dose of insecticide can be reduced, and environmental pollution can be reduced. At present, the mechanism of the insecticidal synergistic effect of echinacoside is not clear, and it is also not used in the control of lice of plants. SUMMARY

[0009] To achieve the above-mentioned purpose, the present application aims to provide a poplar woodlouse control agricultural composition by synergizing flupyradifurone with echinacoside. The active ingredient of the agricultural composition is composed of echinacoside and flupyradifurone, and the mixing mass ratio of flupyradifurone to echinacoside is 1:1-37.5. The present application uses echinacoside which has no same or similar action mechanism with flupyradifurone to mix with flupyradifurone to obtain a composition, which can achieve synergistic effect, effectively improve insecticidal effect, reduce and delay resistance development, reduce the use amount of chemical insecticide flupyradifurone, and reduce environmental pollution.

[0010] In one aspect, the present application relates to an agricultural composition for controlling poplar woodlouse. The active ingredient of the agricultural composition is composed of echinacoside and flupyradifurone, and the mass ratio of flupyradifurone to echinacoside is 1:1-37.5.

[0011] Preferably, in the agricultural composition for controlling poplar woodlouse provided by the present application, the mass ratio of flupyradifurone to echinacoside is 1:10-32.5.

[0012] More preferably, in the agricultural composition for controlling poplar woodlouse provided by the present application, the mass ratio of flupyradifurone to echinacoside is 1:22.5-30.

[0013] More preferably, in the agricultural composition for controlling poplar woodlouse provided by the present application, the mass ratio of flupyradifurone to echinacoside is 1:27.5.

[0014] In another aspect, the present application relates to the use of a composition for controlling Pityococcus drupes, comprising: allowing the composition for controlling Pityococcus drupes to act on Pityococcus drupes and / or their hosts.

[0015] Further, the pesticide containing the composition for controlling Pityococcus drupes is prepared into a suspension agent, an emulsion-in-water agent or an emulsifiable concentrate agent for the convenience of field use.

[0016] The above-mentioned dosage forms also contain an auxiliary agent required for the preparation of the pesticide, which can be one or a mixture of several of the following: a solvent, an emulsifier, a wetting agent, a stabilizer, a dispersant, a thickening agent, a pH adjuster, an antifoaming agent, an antifreeze agent, a filler, etc. The auxiliary agent of the present application can all be known substances, such as various auxiliary agents commonly used in pesticide preparations, which can vary according to different circumstances and are not particularly limited.

[0017] Compared with the prior art, the technical solution provided by the present application at least has the following beneficial effects or advantages:

[0018] The present application first applies the environment-friendly raw material echinacoside to the control of Pityococcus drupes, and first uses echinacoside in combination with the chemical insecticide flupyradifurone, finds that echinacoside has a synergistic effect on flupyradifurone, finds that the activity coefficient of the mixture of flupyradifurone and echinacoside is 2.63 at a ratio of 1:27.5, and the field insecticidal efficiency is as high as 93.36%, which indicates that the use of echinacoside as a synergist in combination with flupyradifurone can greatly reduce the amount of effective ingredients of chemical pesticides, fully exert the efficacy of flupyradifurone, minimize the probability of pest resistance, and reduce environmental pollution caused by long-term use. DETAILED DESCRIPTION

[0019] In the following, the technical solution of the present application will be described in conjunction with examples, but the present application is not limited to the following examples. The experimental methods and detection methods described in each example are all conventional methods unless otherwise specified; and the reagents and materials described are all commercially available unless otherwise specified.

[0020] Echinacoside: purchased from Nanjing Tianlun Biotechnology Co., Ltd., purity specification 90%, 80 mesh sieved powder.

[0021] Flupyradifurone: purchased from Shaanxi Liedu Pharmaceutical Chemical Co., Ltd., purity specification 98%.

[0022] Example 1

[0023] This example is a toxicity test experiment of the mixture of flupyradifurone and echinacoside on Pityococcus drupes.

[0024] Indoor toxicity determination: toxicity determination of echinacoside synergizing flupyradifurone for controlling Pityococcus drupes.

[0025] Test insects: Populus euphratica woodlouse 1st instar nymphs.

[0026] Test agent treatment: The test agent was first dissolved in dimethyl sulfoxide, then diluted with 0.1% Tween-80 aqueous solution to prepare single agent stock solution and various ratio mixed agent stock solutions. Different concentration gradients of single agent and mixed agent were prepared for standby.

[0027] Experimental method: 1st instar nymphs were placed on fresh Populus euphratica wood leaves, 10 nymphs per leaf (50 nymphs per group, repeated 3 times), and the test agent was sprayed in order of low to high concentration, with clean water as blank control. The leaf surface was evenly sprayed with the agent, and then naturally air-dried, wrapped with wet degreased cotton around the petiole, and placed in a culture dish. The number of dead nymphs was counted, and the mortality rate was checked after 24 hours. The death standard was that the insect body had no reaction when touched with a pen nib. The blank control mortality rate was less than 10% for effective test.

[0028] Data analysis: Regression analysis was performed on the logarithmic values of each agent concentration and the corresponding mortality rate, and the LC 50 of each agent was calculated. The activity coefficient was calculated, and the test results are shown in Table 1.

[0029] Activity coefficient = (LC 50 of flupyradifurone) / (LC 50 of flupyradifurone and echinacoside complex)

[0030] The standard for determining whether there is synergistic effect is as follows: activity coefficient = 1 indicates no synergistic effect, activity coefficient > 1 indicates synergistic effect, and activity coefficient < 1 indicates antagonistic effect.

[0031] Through the above test, the toxicity regression curve of flupyradifurone on Populus euphratica woodlouse was y = 1.5399x - 3.1147, with a correlation coefficient of 0.996 and an LC50 of 105.36 mg / L. When the test concentration of echinacoside was increased to 300 mg / L, it still had a survival rate of 85.82% (survival rate = 1 - mortality rate), indicating that echinacoside had no obvious toxic effect on 1st instar nymphs of Populus euphratica woodlouse.

[0032] Table 1: Toxicity test results of flupyradifurone and echinacoside mixed with Populus euphratica woodlouse

[0033] Fluoropyridone 105.35 / Fluoropyridone: echinacoside = 1:0.1 157.24 0.67 Fluoropyridone: echinacoside = 1:0.5 133.35 0.79 Fluoropyridone: echinacoside = 1:1 89.28 1.18 Fluoropyridone: echinacoside = 1:5 74.19 1.42 Fluoropyridone: echinacoside = 1:10 63.85 1.65 Fluoropyridone: echinacoside = 1:15 55.74 1.89 Fluoropyridone: echinacoside = 1:20 50.89 2.07 Fluoropyridone: echinacoside = 1:22.5 47.89 2.20 Fluoropyridone: echinacoside = 1:25 44.83 2.35 Fluoropyridone: echinacoside = 1:27.5 40.06 2.63 Fluoropyridone: echinacoside = 1:30 45.61 2.31 Fluoropyridone: echinacoside = 1:31.75 54.03 1.95 Fluoropyridone: echinacoside = 1:32.5 67.97 1.55 Fluoropyridone: echinacoside = 1:35 79.21 1.33 Fluoropyridone: echinacoside = 1:37.5 90.04 1.17 Fluoropyridone: echinacoside = 1:40 150.50 0.70 Fluoropyridone: echinacoside = 1:50 191.55 0.55 Fluoropyridone: echinacoside = 1:60 Fluoropyridone: echinacoside = 1:70 210.70 0.50

[0034] The results of the toxicity test of the mixture of flupyradifurone and echinacoside on P. populi are shown in Table 1. When the mass ratio of flupyradifurone and echinacoside is in the range provided in the present application, the activity coefficients are all greater than 1 when the mass ratio is 1:1-37.5, and the synergistic effect of echinacoside on the insecticidal effect of flupyradifurone is very significant. In particular, when the mass ratio of flupyradifurone and echinacoside is 1:22.5-30 after mixing, the activity coefficient reaches more than 2.2; when the mass ratio of flupyradifurone and echinacoside is 1:27.5 after mixing, the activity coefficient reaches 2.63, and the effect is the best. The above results show that the use of echinacoside as a synergist in combination with flupyradifurone can greatly reduce the amount of effective ingredients of pesticides, fully exert the efficacy of flupyradifurone, slow down the probability of pest resistance as much as possible, improve the control effect of flupyradifurone on P. populi, and show a synergistic effect.

[0035] Preparation Example 1

[0036] The present example provides a water emulsion of an agricultural composition for controlling P. populi.

[0037] Flupyradifurone 1 part, echinacoside 1 part, trimethylbenzene 5 parts, cyclohexanone 5 parts, castor oil polyoxyethylene ether 8 parts, glycerol fatty acid ester polyoxyethylene ether 5 parts, sodium citrate 1 part, and propylene glycol 2 parts are weighed out in parts by mass. The above active ingredients, solvents, emulsifiers, dispersants, and stabilizers are mixed to form an oil phase, and the antifreezing agent is dissolved in water to form an aqueous phase. The oil phase is slowly added to the aqueous phase under the action of a shearing machine, and shearing is continued for about 30 minutes to prepare a water emulsion of an agricultural composition for controlling P. populi.

[0038] Preparation Example 2

[0039] The present example provides an emulsifiable concentrate of an agricultural composition for controlling P. populi.

[0040] Flupyradifurone 1 part, echinacoside 10 parts, trimethylbenzene 5 parts, cyclohexanone 5 parts, polyoxyethylene fatty alcohol ether 10 parts, and double kill 2 parts are weighed out in parts by mass. The above active ingredients are added to the solvent and dissolved, and then the emulsifier and synergist are added and stirred uniformly to form a uniform transparent oily liquid, which is an emulsifiable concentrate of an agricultural composition for controlling P. populi.

[0041] Preparation Example 3

[0042] The present example provides a water emulsion of an agricultural composition for controlling P. populi.

[0043] Fluorofurane 1 part, echinacoside 20 parts, mesitylene 5 parts, cyclohexanone 5 parts, castor oil polyoxyethylene ether 10 parts, glycerol fatty acid ester polyoxyethylene ether 5 parts, sodium citrate 1 part, glycerol 2 parts, and deionized water 30 parts are weighed out according to the quality parts. The above active ingredients, solvent, emulsifier, dispersant, and stabilizer are mixed to form an oil phase, and the antifreeze is dissolved in water to form an aqueous phase. The oil phase is slowly added to the aqueous phase under the action of a shearing machine, and shearing is continued for about 30 minutes to prepare an agricultural composition of water emulsion for preventing and treating poplar lerp psyllid.

[0044] Preparation Example 4

[0045] This example provides an agricultural composition of suspension for preventing and treating poplar lerp psyllid.

[0046] Fluorofurane 10 parts, echinacoside 225 parts, sodium dodecyl sulfonate 60 parts, methylene bis naphthalene sulfonate sodium 50 parts, polycarboxylate 30 parts, propylene glycol 50 parts, carboxymethyl cellulose 10 parts, and silicon oil 10 parts are weighed out according to the quality parts. The above active ingredients, humectant, dispersant, antifreeze, thickening agent, and defoaming agent are dispersed by high-speed shearing, sanding in a sand mill, and a highly dispersed and stable suspension system is formed in the aqueous medium to prepare an agricultural composition of suspension for preventing and treating poplar lerp psyllid.

[0047] Preparation Example 5

[0048] This example provides an agricultural composition of suspension for preventing and treating poplar lerp psyllid.

[0049] Fluorofurane 10 parts, echinacoside 275 parts, sodium dodecyl sulfonate 60 parts, methylene bis naphthalene sulfonate sodium 40 parts, polycarboxylate 30 parts, propylene glycol 50 parts, carboxymethyl cellulose 10 parts, and silicon oil 10 parts are weighed out according to the quality parts. The above active ingredients, humectant, dispersant, antifreeze, thickening agent, and defoaming agent are dispersed by high-speed shearing, sanding in a sand mill, and a highly dispersed and stable suspension system is formed in the aqueous medium to prepare an agricultural composition of suspension for preventing and treating poplar lerp psyllid.

[0050] Preparation Example 6

[0051] This example provides an agricultural composition of suspension for preventing and treating poplar lerp psyllid.

[0052] According to the mass fraction, take flupyradifurone 10 parts, echinacoside 375 parts, sodium dodecyl sulfonate 60 parts, methylene bis naphthalene sulfonate sodium 40 parts, polycarboxylate 50 parts, propylene glycol 80 parts, carboxymethyl cellulose 10 parts, silicon oil 10 parts, and deionized water 300 parts. The active ingredient, humectant, dispersant, antifreeze, thickening agent and defoaming agent are dispersed by high-speed shearing and sanding in a sand mill. A highly dispersed and stable suspension system is formed in the aqueous medium. A suspension concentrate of an agricultural composition for preventing and controlling poplar lerp moth is prepared.

[0053] Example 7

[0054] The present example provides a suspension concentrate of an agricultural composition for preventing and controlling poplar lerp moth.

[0055] According to the mass fraction, take flupyradifurone 10 parts, echinacoside 375 parts, sodium dodecyl sulfonate 60 parts, methylene bis naphthalene sulfonate sodium 40 parts, polycarboxylate 50 parts, propylene glycol 80 parts, carboxymethyl cellulose 10 parts, silicon oil 10 parts, and deionized water 300 parts. The active ingredient, humectant, dispersant, antifreeze, thickening agent and defoaming agent are dispersed by high-speed shearing and sanding in a sand mill. A highly dispersed and stable suspension system is formed in the aqueous medium. A suspension concentrate of an agricultural composition for preventing and controlling poplar lerp moth is prepared.

[0056] Preparation Example 8

[0057] The present example provides a suspension concentrate of an agricultural composition for preventing and controlling poplar lerp moth.

[0058] According to the mass fraction, take flupyradifurone 10 parts, echinacoside 375 parts, sodium dodecyl sulfonate 60 parts, methylene bis naphthalene sulfonate sodium 40 parts, polycarboxylate 50 parts, propylene glycol 80 parts, carboxymethyl cellulose 10 parts, silicon oil 10 parts, and deionized water 300 parts. The active ingredient, humectant, dispersant, antifreeze, thickening agent and defoaming agent are dispersed by high-speed shearing and sanding in a sand mill. A highly dispersed and stable suspension system is formed in the aqueous medium. A suspension concentrate of an agricultural composition for preventing and controlling poplar lerp moth is prepared.

[0059] Example 2

[0060] The present example provides a field test of flupyradifurone and echinacoside for preventing and controlling poplar lerp moth

[0061] Prepare the contrast agent: flupyradifurone with a purity of 98% purchased from Shaanxi Liedu Pharmaceutical Chemical Co., Ltd. is first dissolved in dimethyl sulfoxide, and then diluted with 0.1% Tween-80 aqueous solution to prepare 17% flupyradifurone as a contrast agent for use.

[0062] The preparation example 1, the preparation example 2, the preparation example 4, the preparation example 5, the preparation example 7, the preparation example 8 and the comparative agent are diluted with water at a ratio of 1:1000 to prepare different groups of liquid medicine, which are used for pest control subsequently. In late May when the first generation of nymphs appears on the poplar, the diluted liquid medicine is sprayed on the branches and leaves of the poplar according to the above grouping. The number of surviving nymphs is investigated 24h, 48h and 72h after the spraying of the liquid medicine. The mortality rate of the nymphs is calculated according to the following formula, and the statistical data are used to analyze the effect of the flupyradifurone and the echinacoside on the control of the poplar psylla in the field. No phytotoxicity of the liquid medicine on the poplar is observed after the spraying of the liquid medicine. The statistical results of the efficacy are shown in Table 2.

[0063] Mortality rate (%) = (number of dead insects ÷ number of live insects) x 100

[0064] Table 2: Field test results of flupyradifurone and echinacoside for the control of poplar psylla

[0065]

[0066] The field test results show that the field test results are consistent with the indoor efficacy results, and the flupyradifurone and the echinacoside at a mass ratio of 1:27.5 have the best insecticidal effect. The insecticidal effect of the combination of the flupyradifurone and the echinacoside is better than that of the comparative agent. The efficacy of the combination of the flupyradifurone and the echinacoside on the poplar psylla increases with the extension of time, and the insecticidal efficiency can reach 93.36% after 72h of spraying.

[0067] The above-described examples are part of the embodiments of the present application, but not all the embodiments. The detailed description of the embodiments of the present application is not intended to limit the scope of the claimed application, but to represent selected embodiments of the present application. All other embodiments obtained by the related deduction and replacement of those skilled in the art under the condition of the concept of the present application, without making creative efforts, belong to the scope of protection of the present application.

Claims

1. An agricultural composition for controlling poplar psyllids, characterized in that, The active ingredient of the agricultural composition is composed of flutriafol and echinacoside, and the mass ratio of flutriafol to echinacoside is 1:1-37.

5.

2. The agricultural composition for controlling P. clusiae according to claim 1, wherein The mass ratio of flutriafol to echinacoside is 1:10-32.

5.

3. The agricultural composition for controlling P. clusiae according to claim 2, wherein The mass ratio of flutriafol to echinacoside is 1:22.5-30.

4. The agricultural composition for controlling P. clusiae according to claim 3, wherein The mass ratio of flutriafol to echinacoside is 1:27.

5.

5. A method for controlling poplar psyllids, characterized in that, The agricultural composition for preventing and treating T. salicina in any one of claims 1-4 is applied to T. salicina and / or their hosts.

6. Use of the agricultural composition for controlling P. clusiae according to any one of claims 1 to 4 for the preparation of a pesticide, characterized in that, The pesticide comprises the agricultural composition for preventing and treating T. salicina.

7. Use according to claim 6, characterized in that, The pesticide is a suspension concentrate.

8. Use according to claim 6, characterized in that, The pesticide is an emulsion in water.

9. Use according to claim 6, characterized in that, The pesticide is an emulsifiable concentrate.

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