A nutritional composition and application thereof that helps to enhance and / or improve individual social behavior

Abstract

The present invention provides a nutritional composition and its application that helps to enhance and / or improve the social behavior of an individual. The composition of the present invention includes DHA, ARA and nervonic acid, wherein the mass ratio of DHA to ARA is 0.2-5:1, and the mass ratio of the total amount of DHA and ARA to nervonic acid is 0.8-20:1. The present invention also provides a food containing the composition, and the application of the composition or food in the preparation of a product that helps to enhance the social behavior of an individual.

Description

Technical Field

[0001] The present invention relates to the technical field of functional nutrient research, and in particular to a nutrient composition and application thereof that is helpful in enhancing and / or improving individual social behavior. Background Art

[0002] Social behavior refers to an individual's ability to interact with people around him and to take care of himself, and is included in the evaluation dimensions of WS / T 580-2017 "Developmental Behavior Assessment Scale for Children Aged 0 to 6". Early childhood is a sensitive and critical period for the development of individual social behavior. The development of good social behavior has a long-term impact on the healthy growth and socialization process of children, and is of great significance to the development of other individual adaptability.

[0003] After searching, there are few reports in the prior art on whether diet affects individual social behavior and what kind of impact it has on individual social behavior. Summary of the invention

[0004] An object of the present invention is to provide a composition that helps to enhance and / or improve the social behavior of an individual.

[0005] Another object of the present invention is to provide related applications of the composition.

[0006] The inventors of this case have discovered that a composition comprising DHA, ARA and neuraminic acid can affect the social behavior of an individual. Combining DHA, ARA and neuraminic acid in a certain proportion has a synergistic effect on enhancing and / or improving the social behavior of an individual.

[0007] On the one hand, the present invention provides a nutritional composition comprising DHA, ARA and nervonic acid, wherein the mass ratio of DHA to ARA is 0.2-5:1, and the mass ratio of the total amount of DHA and ARA to nervonic acid is 0.8-20:1.

[0008] According to a specific embodiment of the present invention, in the composition of the present invention, the mass ratio of DHA to ARA is 0.3-3:1, and the mass ratio of the total amount of DHA and ARA to neuraminic acid is 0.8-20:1.

[0009] According to some specific embodiments of the present invention, in the composition of the present invention, the mass ratio of DHA to ARA is preferably 0.5-2.5:1.

[0010] According to some specific embodiments of the present invention, in the composition of the present invention, the mass ratio of DHA to ARA is preferably 0.5-2:1.

[0011] According to some specific embodiments of the present invention, in the composition of the present invention, the mass ratio of DHA to ARA is preferably 0.5-1.5:1, for example 0.5:1, 0.6:1, 0.67:1, 0.8:1, 1:1, 1.1:1, 1.2:1, 1.3:1 or 1.4:1.

[0012] According to some specific embodiments of the present invention, in the composition of the present invention, the mass ratio of the total amount of DHA and ARA to neuraminic acid is 0.8-10:1.

[0013] According to some specific embodiments of the present invention, in the composition of the present invention, the mass ratio of the total amount of DHA and ARA to neuraminic acid is 0.8-8:1.

[0014] According to some preferred specific embodiments of the present invention, in the composition of the present invention, the mass ratio of the total amount of DHA and ARA to neuraminic acid is 0.8-5:1, further preferably 0.8-3:1, for example 0.9:1, 1:1, 1.1:1, 1.2:1, 1.3:1, 1.5:1, 2:1, 2.5:1 or 3:1.

[0015] According to some specific embodiments of the present invention, in the composition of the present invention, the mass ratio of the total amount of DHA and ARA to neuraminic acid is 10-20:1, for example, 10:1, 11:1, 12:1, 13:1, 14:1, 15:1, 16:1, 17:1, 18:1, 19:1 or 20:1.

[0016] In the above nutritional composition, the raw material for providing the DHA may be DHA oil and / or powder derived from one or a combination of two or more of algae, tuna oil, fish oil, milk-derived DHA from feed nutritional regulation and biotransformation, egg yolk extract and krill oil; wherein the algae may be one or a combination of two or more of Schizochytrium, Okenichthys and Crypthecodinium. Unless otherwise specified, the ratio of DHA to other functional components in the composition of the present invention is based on the content of DHA in the raw material.

[0017] In the above nutritional composition, the raw material of ARA can be one or more raw materials from algae and other oils containing ARA. Unless otherwise specified, the ratio of ARA to other functional components in the composition of the present invention is based on the content of ARA in the raw material.

[0018] In the above nutritional composition, the raw material for providing the nervonic acid can be one or a combination of two or more of nervonic acid derived from cis-15-tetracosenoic acid, Acer truncatum seed oil, nervonic acid synthesized by microbial fermentation, and other oils containing nervonic acid. Unless otherwise specified, the ratio of the nervonic acid to other functional components in the composition of the present invention is based on the content of nervonic acid in the raw material.

[0019] In another aspect, the present invention further provides a food, which contains the above composition of the present invention.

[0020] According to a specific embodiment of the present invention, the food of the present invention can be a common food or a functional food. More specifically, the food is a snack, a nutritional supplement, a health food or a special medical formula food; the present invention does not make special requirements on the specific form of the food, for example, the snack food can be a milk tablet, candy, etc., and the nutritional supplement, dietary supplement health food or special medical formula food can be in the form of oral liquid, powder, capsule, etc.

[0021] According to a specific embodiment of the present invention, the food of the present invention is a dairy product, such as infant formula, milk powder, milk tablets, cheese, liquid dairy products, complementary foods or nutritional supplements. The food of the present invention is not limited to food for infants or children, but can also be food for adults or middle-aged and elderly people.

[0022] According to a specific embodiment of the present invention, in the food of the present invention, the amount of ARA contained in the food is 10-420 mg / 100 g of dry matter of the food, calculated based on the amount of ARA in the composition.

[0023] According to some specific embodiments of the present invention, in the food of the present invention, the amount of ARA contained in the food is 10-200 mg / 100 g of dry food matter, based on the amount of ARA in the composition. According to some more specific embodiments of the present invention, in the food of the present invention, the amount of ARA contained in the food is about 30, about 60 mg / 100 g of dry food matter, about 65 mg / 100 g of dry food matter, about 85 mg / 100 g of dry food matter, about 120 mg / 100 g of dry food matter, about 150 mg / 100 g of dry food matter, based on the amount of ARA in the composition.

[0024] According to some specific embodiments of the present invention, in the food of the present invention, the amount of ARA contained in the food is 15-400 mg / 100 g of dry matter of the food, calculated based on the amount of ARA in the composition.

[0025] In some specific embodiments of the present invention, in the food of the present invention, the amount of ARA contained in the food is 20-150 mg / 100g food dry matter, 100-200 mg / 100g food dry matter, 200-300 mg / 100g food dry matter or 300-400 mg / 100g food dry matter, based on the amount of ARA in the composition.

[0026] In some specific embodiments of the present invention, the content of DHA and neuraminic acid in the food of the present invention can be determined according to the ratio with ARA.

[0027] It is understandable that for different types of food, the content of each functional substance in the final product should be appropriately adjusted within the range permitted by relevant standards and specifications.

[0028] On the other hand, the present invention also provides a use of the above-mentioned composition of the present invention or the above-mentioned food in the preparation of a product that helps to enhance and / or improve the social behavior of an individual.

[0029] According to a specific embodiment of the present invention, in the present invention, the enhancing and / or improving the social behavior of an individual includes: enhancing the social willingness and / or social ability of an individual.

[0030] According to a specific embodiment of the present invention, in the present invention, the social ability includes social interaction ability and / or social adaptability (the ability of an individual to respond and adapt to the social environment and / or social needs around him / her).

[0031] According to a specific embodiment of the present invention, the composition of the present invention or the above-mentioned food is used in the preparation of a product that helps to enhance and / or improve the social behavior of an individual, and the individual is an infant, a toddler or a child.

[0032] In the present invention, unless otherwise specified, the infant refers to an infant of 0-12 months old; the toddler refers to an infant of 1-3 years old; the child refers to a child who is 36 months old but under 15 years old, especially a child under 7 years old.

[0033] According to some specific embodiments of the present invention, in the present invention, the product can be any of the aforementioned foods, or a food obtained by further processing using the aforementioned composition and / or food as raw materials, without adding or with adding other substances.

[0034] According to some specific embodiments of the present invention, in the present invention, the product can be a medicine, and the medicine is used to treat and / or improve the social behavior of an individual, especially to treat and / or improve the low social behavior ability of an individual. In addition to the DHA, ARA and neuraminic acid, the medicine of the present invention can also include pharmaceutically acceptable excipients, and the excipients include excipients, diluents, fillers and / or absorption enhancers. The medicine can be in different forms according to the needs of the recipient. For example, powders, tablets, granulations, microcapsules and / or liquid preparations, etc.

[0035] The nutritional composition and food containing the composition provided by the present invention are helpful to enhance and / or improve the social behavior of an individual, and there is a certain synergistic effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 Display the detection results of the convergence frequency of each experimental group.

[0037] Figure 2 Displays the detection results of the approach cumulative time of the experimental examples in each experimental group.

[0038] Figure 3 Displays the test results of the body contact frequency of the experimental examples in each experimental group.

[0039] Figure 4 Displays the test results of the cumulative body contact time of the experimental examples in each experimental group.

[0040] Figure 5 The detection results of the relative movement frequency of the experimental examples in each experimental group are displayed.

[0041] Figure 6 The detection results of the relative movement cumulative time of the experimental examples in each experimental group are displayed. DETAILED DESCRIPTION

[0042] Before further describing the specific embodiments of the present invention, it should be understood that the scope of protection of the present invention is not limited to the specific embodiments described below; it should also be understood that the terms used in the examples of the present invention are for describing the specific embodiments rather than for limiting the scope of protection of the present invention.

[0043] When numerical ranges are given in the embodiments, it should be understood that the two endpoints of each numerical range and any numerical value between the two endpoints can be selected unless otherwise specified in the present invention.

[0044] Unless defined otherwise, all technical and scientific terms used herein have the same meanings as commonly understood by one of ordinary skill in the art.

[0045] In addition to the specific methods, equipment, and materials used in the embodiments, based on the understanding of the prior art by those skilled in the art and the description of the present invention, any methods, equipment, and materials of the prior art that are similar or equivalent to the methods, equipment, and materials described in the embodiments of the present invention may also be used to implement the present invention.

[0046] Unless otherwise stated, the experimental methods, detection methods, and preparation methods disclosed in the present invention all adopt conventional techniques in the technical field.

[0047] In each embodiment and experiment, the DHA, ARA and neuraminic acid used are all commercially available food-grade raw materials. Unless otherwise specified, the DHA, ARA and neuraminic acid mentioned in the present invention are measured based on the effective content of the functional components DHA, ARA and neuraminic acid in the raw materials.

[0048] Experimental study on the efficacy of nutritional combination in improving social ability

[0049] 1. Bee species and groups

[0050] One-day-old Italian honey bees (Apis mellifera) were used. On the basis of the feed honey bee diet, the intervention methods of different experimental groups are shown in Table 1.

[0051] Table 1

[0052]

[0053] 2. Basic honey bee cultivation

[0054] 2.1 Diet preparation

[0055] Preparation of diet for the control group: weigh 500 g of sucrose and 10 g of casein hydrolysate, dilute to 1 L with ultrapure water, stir to dissolve, filter and sterilize with a 0.22 μm filter membrane to obtain the 50% (w / v) sucrose solution containing 1% (w / v) casein hydrolysate required for the experiment.

[0056] Diet of treatment groups: The free-feeding group added the treatment reagent to the diet of the control group; the single-fed group was fed with a single concentrated reagent in 5 μL sterile sugar water, with each bee receiving a daily diet of 40 μL sugar water.

[0057] 2.2 Bee picking

[0058] (1) Selection of honeycombs

[0059] Honeycomb spleens of appropriate age were selected from the apiary, quickly transported back to the laboratory, and cultured in an incubator (DHP-9162 constant temperature incubator of Zhongyi Guoke (Beijing) Technology Co., Ltd.) at a temperature of 35°C and a humidity of 60-80%.

[0060] (2) Bee picking

[0061] Starting from the second day, observe the status of the emerging bees every day (if they have not emerged, continue to culture them). Gently remove the emerging bees with tweezers and divide them into disposable transparent culture cups, 30 bees per cup. Divide them into enough cups according to the experimental needs.

[0062] 2.3 Bee pretreatment

[0063] (1) Preparation of intestinal fluid

[0064] The fledged bees were taken directly from the apiary, and the intestines were extracted. After adding 100ul PBS buffer to each intestine and grinding, 50% glycerol was added in a 1:1 ratio and stored at -80 degrees Celsius.

[0065] (2) Intestinal fluid colonization

[0066] Take a sterile 2mL centrifuge tube, add 990μL sterile PBS buffer, add 10μL worker bee intestinal fluid, mix well, and finally use 50% (w / v) sterile sucrose solution to make up to 2ml mark, shake and mix well. Feed each cup of newly emerged bees with 1 tube of the above mixed solution and continue to culture in a constant temperature incubator for 24h.

[0067] 2.4 Handling and feeding

[0068] After the colonization of intestinal fluid was completed, treatment feeding began. The CV group was fed with the CV diet, and the treatment group was fed with the treatment diet. Treatment feeding continued for 7 days. During this period, the growth status of the bees was observed daily and the diet was changed in time to ensure adequate diet. The culture conditions were: dark, 35 degrees Celsius, and 60%-80% humidity.

[0069] If the animals are gavaged individually, the diet placed in the feeding cup is CV diet, and only different treatment agents are added to the gavage-fed diet.

[0070] 3. Experimental testing

[0071] Social skills test:

[0072] (1) Preparation of the behavioral observation room: Turn on the air conditioner and the exhaust fan of the experimental table 1-2 hours before the experiment to keep the temperature of the observation room (26°C) constant and to ensure that there is no odor in the experimental table.

[0073] (2) Place the experimental bees in groups of three in a transparent, breathable open field that has been prepared in advance.

[0074] (3) The open field was allowed to adapt to the experimental table for 15 minutes, and then a video of the open field was filmed from above for 45 minutes.

[0075] (4) The above videos were analyzed using the Etho Vision XT 17 animal social behavior analysis system to obtain animal social behavior ability data, and social behavior was evaluated from two dimensions: social interaction tendency evaluation and social interaction evaluation.

[0076] 4. Experimental Results

[0077] (1) Results of social interaction tendency evaluation

[0078] The evaluation of social interaction tendency is the frequency and time of experimental animals approaching each other but not engaging in social behavior. The present invention uses approach frequency and cumulative approach time to evaluate the ability to tend to socialize (i.e., reflecting the "social willingness" described in the present invention).

[0079] The approaching frequencies of each experimental case are as follows Figure 1As shown in the figure, compared with Experimental Example 1, there is no significant difference in the approach frequency of Experimental Example 7; the approach frequencies of Experimental Examples 2 and 3 have an increasing trend, but there is no significant difference; the approach frequency indicators of Experimental Examples 4, 5 and 6 have increased significantly. Compared with Experimental Example 3, Experimental Example 6 has an increasing trend, but it is not significant. Compared with Experimental Example 7, the approach frequency of Experimental Example 6 has increased significantly.

[0080] The cumulative approach time of each experimental case is as follows: Figure 2 As shown, compared with Experimental Example 1, there is no significant difference in the approach frequency of Experimental Examples 2-5 and Experimental Example 7, and only the approach cumulative time of Experimental Example 6 is significantly increased; compared with Experimental Examples 3 and Experimental Examples 7, the approach frequency of Experimental Example 6 is significantly increased.

[0081] In summary, in terms of social interaction tendency, at the experimental dose, neuraminic acid intervention alone had no significant effect on approach frequency and cumulative approach time; different proportions of DHA and ARA intervention groups had an effect on approach frequency; combined intervention of DHA, ARA and neuraminic acid significantly increased both approach frequency and cumulative approach time, indicating that the nutritional combination of DHA, ARA and neuraminic acid has an effect on nerve and brain development, promotes social interaction tendency, and has a certain synergistic technical effect on approach frequency and cumulative approach time.

[0082] (2) Social interaction evaluation

[0083] Social interaction evaluation indicates the frequency and duration of actual social interaction of experimental animals. It is the most intuitive expression of an individual's social ability (i.e., reflecting the "social ability" mentioned in the present invention, more specifically mainly including social interaction ability and / or social adaptability). It is evaluated by the frequency of physical contact, the cumulative time of physical contact, the frequency of relative movement, and the cumulative time of relative movement.

[0084] The frequency of physical contact in each experimental case is as follows Figure 3 As shown in Figure 1, there is no significant difference between the groups compared with Experimental Example 1, but there is an increasing trend in Experimental Examples 3, 5, and 6. The cumulative body contact time of each experimental example is shown in Figure 1. Figure 4 As shown, compared with Experimental Example 1, Experimental Example 2, Experimental Example 4, and Experimental Example 5 had no significant differences, and Experimental Example 7 had a significant decrease; Experimental Example 3 had an increasing trend, but no significant difference; Experimental Example 6 had a significant increase in the cumulative time of physical contact. Compared with Experimental Examples 3 and 7, the cumulative time of physical contact in Experimental Example 6 increased significantly.

[0085] The relative movement frequency of each experimental example is as follows Figure 5As shown in Figure 1, there is no significant difference in Experimental Example 2, Experimental Example 5 and Experimental Example 7 compared with Experimental Example 1; the relative movement frequency of Experimental Example 3, Experimental Example 4 and Experimental Example 6 increased significantly. Compared with Experimental Example 3, Experimental Example 6 has an increasing trend, but it is not significant; compared with Experimental Example 7, the relative movement frequency of Experimental Example 6 increased significantly. The relative movement cumulative time of each experimental example is shown in Figure 1. Figure 6 As shown in the figure, compared with Experimental Example 1, the cumulative relative movement time of Experimental Example 2 and Experimental Example 7 decreased; there was no significant difference between Experimental Example 3, Experimental Example 4 and Experimental Example 5; the cumulative relative movement time of Experimental Example 6 increased significantly. Compared with Experimental Example 3 and Experimental Example 7, the cumulative relative movement time of Experimental Example 6 increased significantly.

[0086] In terms of social interaction indicators, at the experimental dose, intervention with neuraminic acid alone had no significant effect on the frequency of physical contact and the frequency of relative movement, while the cumulative time of physical contact and the cumulative time of relative movement both decreased; intervention groups with different proportions of DHA and ARA had different effects on the frequency of physical contact, the cumulative time of physical contact, the frequency of relative movement and the cumulative time of relative movement, suggesting that the effects of DHA and ARA on social interaction are related to the proportions; after the combined intervention of DHA, ARA and neuraminic acid, the frequency of physical contact, the cumulative time of physical contact, the frequency of relative movement and the cumulative time of relative movement all increased significantly, suggesting that the nutritional combination of DHA, ARA and neuraminic acid has an impact on the development of nerves and brain, and promotes social interaction, and has a certain synergistic technical effect on the social interaction indicators of body contact frequency, cumulative time of body contact, relative movement frequency and cumulative time of relative movement.

[0087] Compared with Experimental Example 1, Experimental Examples 2-5 fortified DHA and ARA in different proportions on the basis of normal diet. The results showed that Experimental Examples 2-5 increased in approach frequency, but only Experimental Examples 4 and 5 had significant differences in increase; there was no significant difference in approach cumulative time in Experimental Examples 2-5, but Experimental Examples 3-5 had an increasing trend; there was no significant difference in body contact frequency and cumulative body contact time in Experimental Examples 2-5, but Experimental Examples 3-5 had an increasing trend; there was no significant difference in relative movement frequency and cumulative relative movement frequency time in Experimental Examples 2, 4 and 5, but the relative movement frequency of Experimental Examples 3-5 had an increasing trend; the cumulative relative movement frequency of Experimental Examples 3 and 5 had an increasing trend. It suggests that different proportions of DHA and ARA have different effects on social behavior. In the experimental examples, the DHA:ARA mass ratio of 1:1 in Experimental Example 3 is the optimal ratio of the two.

[0088] Compared with Experimental Example 1, Experimental Example 7 strengthened neuraminic acid on the basis of a normal diet. The results showed that at the experimental dose and ratio of the present invention, strengthening neuraminic acid alone had no significant effect on the approach frequency, body contact frequency, and relative movement frequency, but could reduce the cumulative approach time, the cumulative body contact time, and the cumulative relative movement time.

[0089] Compared with Experimental Example 1, Experimental Example 6 simultaneously strengthened DHA, ARA and neuraminic acid on the basis of a normal diet. The results showed that the intervention in Experimental Example 6 significantly improved social interaction tendency (approach frequency, cumulative approach time) and social interaction (body contact frequency, cumulative body contact time, relative movement frequency and cumulative relative movement time); and compared with Experimental Example 7 in which neuraminic acid was strengthened alone, the approach frequency, cumulative approach time, cumulative body contact time, relative movement frequency and cumulative relative movement time were also significantly increased; compared with the strengthening of DHA and ARA alone, the cumulative approach time, cumulative body contact time and cumulative relative movement time were significantly increased, and the approach frequency, body contact frequency and relative movement frequency all showed an increasing trend.

[0090] According to the above results, the combined intervention of DHA, ARA and neuraminic acid in a specific proportion achieved the optimal nutritional effect on brain nerves and brain functions, and the combined intervention had an increasing effect on approach frequency, cumulative approach time, body contact frequency, cumulative body contact time, relative movement frequency and cumulative relative movement time, indicating that the nutritional combination of DHA, ARA and neuraminic acid has an effect on nerve and brain development, and promotes both social interaction tendency and social interaction, and there is a certain synergistic technical effect.

[0091] The above description of the embodiments is to facilitate the understanding and use of the invention by those skilled in the art. It is obvious that those skilled in the art can easily make various modifications to these embodiments and apply the general principles described herein to other embodiments without creative work. Therefore, the present invention is not limited to the above embodiments, and improvements and modifications made by those skilled in the art based on the disclosure of the present invention without departing from the scope of the present invention should be within the scope of protection of the present invention.

Claims

1. Use of a nutritional composition or a food containing the composition in preparing a product that helps to enhance and / or improve the social behavior of an individual, wherein: The composition includes DHA, ARA and neuraminic acid, the mass ratio of DHA to ARA is 0.2-5:1, the mass ratio of the total amount of DHA and ARA to neuraminic acid is 0.8-20:1, and the social behavior includes social interaction willingness and / or social interaction ability.

2. The use according to claim 1, characterized in that: The mass ratio of DHA to ARA is 0.3-3:1, and the mass ratio of the total amount of DHA and ARA to neuraminic acid is 0.8-10:1 or 10-20:

1.

3. The use according to claim 2, characterized in that: In the composition, the mass ratio of DHA to ARA is 0.5-2.5:

1.

4. The use according to claim 1, characterized in that: In the composition, the mass ratio of the total amount of DHA and ARA to neuraminic acid is 0.8-5:

1.

5. The use according to claim 1, characterized in that: The food is a snack, a nutritional supplement, a health food or a special medical purpose formula food.

6. The use according to claim 1, characterized in that: The food is a dairy product.

7. The use according to claim 6, characterized in that: The food is infant formula, milk powder, milk tablets, cheese, liquid dairy products, complementary foods or nutritional supplements.

8. The use according to any one of claims 5 to 7, characterized in that: Calculated based on the amount of ARA in the composition, the amount of ARA contained in the food is 10-420 mg / 100 g of food dry matter.

9. The use according to claim 8, characterized in that: The amount of ARA contained in food is 15-400 mg / 100 g of food dry matter.

10. The use according to any one of claims 1 to 7, characterized in that: The individual is an infant, toddler or child.

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