A cell slide preparation and staining integrated machine

Abstract

The present invention relates to the technical field of biological experimental equipment, specifically a cell slide preparation and staining integrated machine, including a machine platform and a centrifugal slide preparation mechanism and a staining mechanism arranged on the machine platform, the centrifugal slide preparation mechanism including a centrifugal disk driven to rotate by a driving device and a centrifugal test tube rotatably arranged on the centrifugal disk, the centrifugal test tube rotates along a first rotation direction around a rotation axis X when the centrifugal disk rotates, and a separation device is arranged between the centrifugal test tube and the centrifugal disk, and the separation device is constructed to be able to apply a force along the first rotation direction to the bottom of the centrifugal test tube when the centrifugal force to which the centrifugal test tube is subjected ends. The present invention arranges a separation device between the centrifugal test tube and the centrifugal disk, and the separation device is able to apply a force along the first rotation direction to the bottom of the centrifugal test tube when the centrifugal force to which the centrifugal test tube is subjected ends, so that the upper layer of liquid in the centrifugal test tube is directly poured out from the tube mouth of the centrifugal test tube, which facilitates the discharge of waste liquid and does not pollute other components.

Description

Technical Field

[0001] The invention relates to the technical field of biological experimental equipment, in particular to an all-in-one machine for cell preparation and staining. Background Art

[0002] With the continuous improvement of pathology technology, second-generation liquid-based cytology preparation methods, represented by sedimentation-based liquid-based cytology preparation, have become increasingly widely used in the field of pathology diagnosis, particularly in the preparation of cytopathology specimens. These technologies improve diagnostic efficiency and accuracy by improving traditional sample processing procedures.

[0003] However, in the prior art, such as the invention patent disclosed in announcement number CN108931414B, a cell preparation and staining all-in-one machine is used to achieve uniform release of waste liquid by setting an opening at the bottom of the centrifuge cylinder and using a positioning column to close the opening, thereby reducing downtime during the operation of the equipment. Specifically, this design allows the positioning column to be fixed on a positioning disk, which is mounted on the centrifuge shaft; the centrifuge cylinder is placed on a centrifuge turntable that can slide along the centrifuge shaft. When the waste liquid needs to be discharged, the positioning column can be separated from the centrifuge cylinder by simply moving the centrifuge turntable away from the positioning disk, and then the bottom opening can be opened to allow the waste liquid to be discharged. This solution effectively simplifies the waste liquid treatment process, but it also brings some new disadvantages: first, the positioning column used to block the waste liquid outlet of the centrifuge cylinder will directly contact the waste liquid, which not only increases the difficulty of cleaning, but may also become one of the factors affecting the working efficiency of the entire system. Secondly, if the positioning column cannot be thoroughly cleaned, there is a risk of contaminating the subsequent cell samples to be processed, which poses a potential threat to ensuring the accuracy and reliability of the experimental results.

[0004] Therefore, in order to solve the above problems, the present invention provides an all-in-one cell preparation and staining machine. Summary of the Invention

[0005] The object of the present invention is to provide an all-in-one cell preparation and staining machine to solve the problems raised in the above background technology.

[0006] The present invention is achieved through the following technical solutions:

[0007] A cell slide preparation and staining machine includes a machine platform and a centrifugal slide preparation mechanism and a staining mechanism arranged on the machine platform. The centrifugal slide preparation mechanism includes a centrifugal disk driven to rotate by a driving device and a centrifugal test tube rotatably arranged on the centrifugal disk. When the centrifugal disk rotates, the centrifugal test tube rotates along a first rotation direction around a rotation axis X. A desoldering device is provided between the centrifugal test tube and the centrifugal disk. The desoldering device is constructed to be able to apply a force along the first rotation direction to the bottom of the centrifugal test tube when the centrifugal force applied to the centrifugal test tube ends.

[0008] Optionally, the centrifugal film-making mechanism further includes a sleeve, which is rotatably arranged on the centrifugal disk around a rotation axis X, a first slot being provided at one end of the sleeve, and a second slot being connected to the first slot being provided at the other end of the sleeve, the centrifugal test tube being a tubular structure with openings at both ends and being pluggable in the first slot, and a glass slide capable of covering the tube mouth of the centrifugal test tube being pluggable in the second slot.

[0009] Optionally, a clearance groove is provided at the communication position between the first slot and the second slot, and the clearance groove shares an opening with the second slot.

[0010] Optionally, the centrifugal slab-making mechanism further includes a U-shaped bracket, the bottom of the bracket is fixed to the centrifugal disc, columnar connecting shafts are provided on opposite sides of the sleeve, and inclined connecting grooves are provided on the two inner side walls of the bracket, the connecting grooves form an opening on the end face of the bracket, and the sleeve is movably connected to the connecting groove via the connecting shaft.

[0011] Optionally, the de-fixing device includes a traction frame, which is rotatably arranged on the bracket around a rotation axis Y and a first torsion spring is provided at the rotation connection position between the two. The free end of the traction frame can be magnetically fixed or clamped to the sleeve after the centrifuge tube is rotated by a predetermined angle along the first rotation direction.

[0012] Optionally, a permanent magnet is fixedly provided on the free end of the traction frame, an iron piece is fixedly provided on the outer wall of the sleeve, and the permanent magnet is magnetically fixed to the iron piece after the centrifugal test tube rotates a predetermined angle along the first rotation direction.

[0013] Optionally, a clip-on frame is fixedly provided on the side wall of the sleeve, and the clip-on frame includes a fixing portion and a folding portion, the first end of the fixing portion is fixedly connected to the outer wall of the sleeve, and the folding portion is rotatably connected to the second end of the fixing portion via a rotating shaft, the second end of the fixing portion is provided with a protrusion for limiting the rotation angle of the folding portion, and a second torsion spring is provided at the rotation connection position between the fixing portion and the folding portion.

[0014] Optionally, the centrifugal slab preparation mechanism further includes a waste liquid tank, which is installed on the centrifugal disk, and the waste liquid in the centrifugal test tube can be discharged into the waste liquid tank after the centrifugal test tube rotates a predetermined angle along the first rotation direction.

[0015] Optionally, the dyeing mechanism includes a rotating cylinder, a lifting cylinder, a connecting frame, a liquid suction device and a dye storage tank, the cylinder body of the rotating cylinder is fixedly mounted on the machine platform, the cylinder body of the lifting cylinder is fixed on the output end of the rotating cylinder, the liquid suction device is fixedly connected to the output end of the lifting cylinder through the connecting frame, and the dye storage tank and the centrifugal test tube are arranged on the rotation path of the liquid suction device.

[0016] Optionally, the liquid suction device includes a shell, a rubber bag, a suction cylinder and a liquid delivery pipe, the shell is fixed on the connecting frame, the rubber bag is arranged in the shell, the suction cylinder is installed on the top of the shell, the output end of the suction cylinder extends into the inner wall of the shell and is fixedly connected to a pressure plate, and the liquid delivery pipe is arranged at the bottom of the shell and is connected to the rubber bag.

[0017] Compared with the prior art, the present invention provides a cell preparation and staining all-in-one machine, which has the following beneficial effects:

[0018] 1. The present invention provides a separation device between the centrifuge tube and the centrifugal disk. The separation device can apply a force in a first rotation direction to the bottom of the centrifuge tube when the centrifugal force applied to the centrifuge tube ends, thereby directly pouring the upper layer of liquid in the centrifuge tube out of the tube mouth of the centrifuge tube, thereby facilitating the discharge of waste liquid and preventing contamination of other components.

[0019] 2. The present invention utilizes a traction frame and a first torsion spring. When the centrifugal disc rotates, the traction frame rotates in the second rotational direction and encounters the sleeve rotating in the first rotational direction. The two are then secured together by magnetic attraction or a snap-fit ​​connection. When the centrifugal disc stops rotating, the traction frame returns to its original position under the rebound force of the first torsion spring, driving the sleeve to continue rotating in the first rotational direction, thereby automatically discharging waste liquid from the centrifugal test tube.

[0020] 3. The present invention provides a clamping frame for use with the traction frame. When the centrifugal disk rotates, the traction frame is clamped together with the clamping frame under the action of centrifugal force. When the centrifugal disk stops rotating, the traction frame drives the sleeve to continue rotating in the first rotation direction through the clamping cooperation between the clamping frame and the traction frame, thereby achieving the purpose of automatically discharging the waste liquid in the centrifugal test tube. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0022] Figure 2 Schematic diagram of the centrifugal slab making mechanism of the present invention;

[0023] Figure 3 Schematic diagram of the cross-sectional structure of the centrifugal slab making mechanism of the present invention;

[0024] Figure 4 Schematic diagram of the connection structure between the centrifugal test tube and the bracket of the present invention;

[0025] Figure 5 For the present invention Figure 4 A schematic diagram of the enlarged structure at point A;

[0026] Figure 6 Schematic diagram of the disassembly structure of the glass slide and the sleeve of the present invention;

[0027] Figure 7 For the present invention Figure 4 Schematic diagram of the cross-section structure;

[0028] Figure 8 Schematic diagram of the structure of the centrifugal test tube and the traction frame of the present invention in three states: before the centrifugal disk rotates, during the rotation, and when the rotation stops;

[0029] Figure 9 It is a partial structural schematic diagram of the dyeing mechanism of the present invention;

[0030] Figure 10 Schematic diagram of the structure of the liquid suction device of the present invention;

[0031] Figure 11 It is a structural schematic diagram of the traction frame and the clamping frame of the present invention;

[0032] Figure 12 It is a structural schematic diagram of the clamping frame of the present invention.

[0033] In the figure: 1. machine; 2. driving device; 3. centrifugal disk; 4. centrifugal test tube; 5. sleeve; 6. first slot; 7. second slot; 8. slide; 9. clearance slot; 10. bracket; 11. connecting shaft; 12. connecting slot; 13. traction frame; 14. first torsion spring; 15. permanent magnet; 16. iron piece; 17. fixing part; 18. folding part; 19. rotating shaft; 20. protrusion; 21. second torsion spring; 22. waste liquid tank; 23. lifting rod; 24. inserting rod; 25. slider; 26. slide; 260. insertion end; 261. limiting end; 27. rotating cylinder; 28. lifting cylinder; 29. ​​connecting frame; 30. dye storage tank; 31. liquid suction device; 310. shell; 311. rubber bag; 312. suction cylinder; 313. liquid delivery pipe; 314. pressure plate. DETAILED DESCRIPTION

[0034] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0035] Example: See Figures 1 to 12 According to an embodiment of the present invention, a cell preparation and staining all-in-one machine is provided, which includes a machine platform 1 and a centrifugal preparation mechanism and a staining mechanism arranged on the machine platform 1. The centrifugal preparation mechanism includes a centrifugal disk 3 driven to rotate by a driving device 2 and a centrifugal test tube 4 rotatably arranged on the centrifugal disk 3. Specifically, the driving device 2 is a servo motor, the outer shell of the servo motor is fixed on the machine platform 1, and the output shaft of the servo motor and the centrifugal disk 3 are coaxially fixed to the centrifugal disk 3 through a flange. When the centrifugal disk 3 rotates, the centrifugal test tube 4 rotates around a rotation axis X along a first rotation direction. A desoldering device is arranged between the centrifugal test tube 4 and the centrifugal disk 3. The desoldering device is constructed to be able to apply a force along the first rotation direction to the bottom of the centrifugal test tube 4 when the centrifugal force applied to the centrifugal test tube 4 ends.

[0036] In the integrated cell preparation and staining machine using the above-described structure, when the centrifugal disc 3 is in a stationary state, the centrifugal tube 4 is in a vertical position with its opening facing upward. At this point, a preservative solution containing a cell sample can be added to the centrifugal tube 4. Next, the centrifugal disc 3 is controlled to rotate, and under the action of centrifugal force, the centrifugal tube 4 rotates about the rotation axis X in a first rotational direction. Because the density of the cells is greater than that of the preservative solution, the cells will gather at the bottom of the centrifugal tube 4, achieving centrifugal sedimentation. After centrifugal sedimentation is completed, the centrifugal disc 3 stops rotating, and the de-immobilization device applies a force in the first rotational direction to the bottom of the centrifugal tube 4, causing the centrifugal tube 4 to maintain its current tilted state or continue to rotate in the first rotational direction. In this way, the upper layer of liquid (i.e., waste liquid) in the centrifugal tube 4 is directly poured out of the tube opening of the centrifugal tube 4, facilitating the discharge of the waste liquid and preventing contamination of other components.

[0037] Combine Figures 4 to 7In this exemplary embodiment, the centrifugal smearing mechanism further includes a sleeve 5, which is rotatably mounted on the centrifugal disk 3 about a rotation axis X. A first slot 6 is provided at one end of the sleeve 5, and a second slot 7 is provided at the other end thereof, which is connected to the first slot 6. The centrifugal test tube 4 is a tubular structure with two open ends. The first end of the centrifugal test tube 4 is removably mounted in the first slot 6, and the second end of the centrifugal test tube 4 is exposed outside the first slot 6. A glass slide 8 is removably mounted in the second slot 7, which is capable of covering the opening of the first end of the centrifugal test tube 4. With this arrangement, when the centrifugal disk 3 begins to rotate, the cells in the centrifugal test tube 4 are attracted toward the first end of the centrifugal test tube 4 under the action of centrifugal force and are ultimately evenly distributed on the glass slide 8, thereby achieving direct centrifugal sedimentation preparation of the cells and eliminating the step of transferring the cells in the centrifugal test tube 4 to the glass slide 8 in the traditional method. In addition, during the entire centrifugation process, due to the action of centrifugal force, the centrifugal test tube 4 will be tightly pressed against the slide 8, ensuring the sealing of the connection between the two, effectively preventing the leakage of the preservation solution, and further improving the safety and efficiency of the preparation process.

[0038] Combine Figure 6 and Figure 7 In this exemplary embodiment, a clearance groove 9 is provided at the connection point between the first slot 6 and the second slot 7. This clearance groove 9 shares an opening with the second slot 7. The provision of this clearance groove effectively prevents scratching between the cell-attaching surface of the slide 8 and the inner wall of the second slot 7, thereby ensuring that the cells on the slide 8 are evenly distributed and intact. This not only protects the quality of the cell sample but also improves the reliability and consistency of the preparation process, resulting in a clearer and more accurate final cell smear, facilitating subsequent microscopic observation and pathological diagnosis.

[0039] Combine Figure 4In this exemplary embodiment, the centrifugal slab making mechanism further includes a U-shaped bracket 10, the bottom of which is fixed to the centrifugal disc 3. A cylindrical connecting shaft 11 is provided on opposite sides of the sleeve 5. An inclined connecting groove 12 is formed on the inner sidewalls of the bracket 10. The connecting groove 12 opens on the end surface of the bracket 10. The sleeve 5 is movably connected to the connecting groove 12 via the connecting shaft 11. In other words, the connecting shaft 11 can rotate and slide within the connecting groove 12. With this arrangement, when the centrifugal tube 4 and the sleeve 5 are rotated in the first rotational direction by centrifugal force, the connecting shaft 11 will always remain at the lower end of the connecting groove 12 due to the inclined arrangement of the connecting groove 12. This ensures the smooth rotation of the centrifugal tube 4 and the sleeve 5 and prevents the connecting shaft 11 from being separated from the connecting groove 12, thereby ensuring the stability and safety of the entire system. In addition, since the connecting groove 12 forms an opening on the end face of the bracket 10, this design not only facilitates the assembly between the sleeve 5 and the bracket 10, but also provides convenience for the cleaning and replacement of the sleeve 5, further improving the maintenance efficiency and operational convenience of the equipment.

[0040] Combine Figure 4 and Figure 5 In this exemplary embodiment, the decoupling device includes a traction frame 13, which is rotatably mounted on the bracket 10 about a rotation axis Y. A first torsion spring 14 is provided at the rotational connection between the two. Specifically, the rotation axis Y can be colinear with the rotation axis X. The free end of the traction frame 13 can be magnetically fixed or snap-fastened to the sleeve 5 after the centrifugal tube 4 rotates in a first rotational direction by a predetermined angle. With this design, when the centrifugal disc 3 begins to rotate, the traction frame 13 overcomes the elastic force of the first torsion spring 14 under the action of centrifugal force and rotates about the rotation axis Y in a second rotational direction opposite to the first rotational direction. It eventually encounters the sleeve 5 rotating in the first rotational direction and is secured thereto by magnetic attraction or snap-fastening. When the centrifugal disc 3 stops rotating, the centrifugal force disappears, and the rebound force of the first torsion spring 14 causes the traction frame 13 to rotate in the first rotational direction, thereby driving the sleeve 5 to continue rotating in the first rotational direction, thereby automatically discharging the waste liquid from the centrifugal tube 4.

[0041] In order to achieve that the free end of the traction frame 13 can be magnetically fixed to the sleeve 5 after the centrifugal tube 4 rotates a predetermined angle along the first rotation direction, in an exemplary embodiment, a permanent magnet 15 is fixedly provided at the free end of the traction frame 13, and an iron piece 16 is fixedly provided on the outer wall of the sleeve 5. The permanent magnet 15 is magnetically fixed to the iron piece 16 after the centrifugal tube 4 rotates a predetermined angle along the first rotation direction. Figure 8The figure shows, from left to right, the three states of the centrifugal tube 4 and the traction frame 13: before the centrifugal disc 3 rotates, during rotation, and when rotation stops. Before the centrifugal disc 3 rotates, the centrifugal tube 4 is in a vertical state, and the free end of the traction frame 13 is at a higher position. During the rotation of the centrifugal disc 3, the centrifugal tube 4 rotates in the first rotation direction until it is almost horizontal. At this time, the permanent magnet 15 fixed to the free end of the traction frame 13 is attracted to the iron piece 16 on the sleeve 5. After the centrifugal disc 3 stops rotating, the centrifugal tube 4 is pulled by the traction frame 13 and continues to rotate in the first rotation direction until all the waste liquid in the centrifugal tube 4 is discharged into the waste liquid tank 22. Of course, as long as the free end of the traction frame 13 can be magnetically fixed to the sleeve 5 after the centrifugal disc 3 rotates in the first rotation direction by a predetermined angle, there is no particular limitation on the specific magnetic fixing method between the traction frame 13 and the sleeve 5. For example, other types of magnetic materials or magnetic structures can be used to achieve this function.

[0042] Combine Figure 11 and Figure 12 To ensure that the free end of the traction frame 13 can be locked and secured to the sleeve 5 after the centrifugal tube 4 has rotated a predetermined angle in the first rotational direction, in another exemplary embodiment, a clamping frame is fixedly mounted on the side wall of the sleeve 5. The clamping frame includes a fixing portion 17 and a folding portion 18. The first end of the fixing portion 17 is fixedly connected to the outer wall of the sleeve 5, and the folding portion 18 is rotatably connected to the second end of the fixing portion 17 via a rotating shaft 19. The second end of the fixing portion 17 is provided with a protrusion 20 that limits the rotation angle of the folding portion 18. A second torsion spring 21 is installed at the rotational connection between the fixing portion 17 and the folding portion 18. With this design, when the centrifugal disc 3 begins to rotate, the traction frame 13 rotates in a second rotational direction opposite to the first rotational direction under the action of centrifugal force. As the traction frame 13 moves, it pushes the folding portion 18 to overcome the elastic force of the second torsion spring 21 and rotate downward about the rotating shaft 19. After the traction frame 13 passes over the folding portion 18, the folding portion 18 rotates upward under the rebound force of the second torsion spring 21. When the centrifugal disc 3 stops rotating, the traction frame 13 and the sleeve 5 are engaged by the engagement between the engaging frame and the traction frame 13, thereby automatically draining the waste liquid from the centrifugal tube 4. Of course, the specific method of engaging and securing the traction frame 13 and the sleeve 5 is not particularly limited, as long as the free end of the traction frame 13 is ensured to be engaged and secured with the sleeve 5 after the centrifugal tube 4 rotates a predetermined angle in the first rotational direction. For example, other types of engaging structures or mechanisms may be used to achieve this function.

[0043] Combine Figure 3In this exemplary embodiment, the centrifugal film making mechanism also includes a waste liquid tank 22, which is mounted on the centrifugal disc 3. The waste liquid in the centrifugal test tube 4 can be discharged into the waste liquid tank 22 after the centrifugal test tube 4 is rotated by a predetermined angle along the first rotation direction, thereby facilitating the centralized treatment and collection of the waste liquid. Specifically, a plurality of brackets 10 are arranged around the center of the centrifugal disc 3, and these brackets 10 support and fix components such as the sleeve 5. The waste liquid tank 22 is set at the center position of the centrifugal disc 3, and a hollow lifting rod 23 is fixed in the waste liquid tank 22, and the lifting rod 23 forms an opening at the bottom of the waste liquid tank 22. A plug rod 24 is vertically fixed at the center position of the centrifugal disc 3. This plug rod 24 can be inserted into the interior of the lifting rod 23 through the opening at the bottom of the lifting rod 23 to play a positioning and fixing role. A T-shaped slider 25 is provided at the bottom of each bracket 10, and a corresponding slide groove 26 is provided on the centrifugal disc 3. The end of the chute 26 near the waste liquid tank 22 is an insertion end 260, and the end opposite the insertion end 260 is a restriction end 261. In specific implementation, the T-shaped slider 25 at the bottom of the bracket 10 is inserted from the insertion end 260 of the chute 26 and then slides toward the restriction end 261. This can limit the vertical position of the bracket 10 on the centrifugal disk 3 and prevent it from moving during the centrifugal process. Next, the waste liquid tank 22 is placed in the space surrounded by multiple brackets 10. The movement of the waste liquid tank 22 in the radial direction of the centrifugal disk 3 is limited by the insertion rod 24. At the same time, the waste liquid tank 22 also limits the movement of the bracket 10 along the radial direction of the centrifugal disk 3, thereby ensuring the stability and reliability of the entire structure. Through this design, the bracket 10 and the waste liquid tank 22 can be easily installed on the centrifugal disk 3. During disassembly, only the waste liquid tank 22 needs to be removed first, and then the bracket 10 can be easily removed by moving it along the center position of the centrifugal disk 3. This design simplifies the assembly and maintenance process of the centrifugal film making mechanism.

[0044] Combine Figure 1 and Figure 9To achieve cell staining, in this exemplary embodiment, the staining mechanism includes a rotating cylinder 27, a lifting cylinder 28, a connecting frame 29, a liquid aspirator 31, and a stain storage tank 30. The cylinder body of the rotating cylinder 27 is fixedly mounted on the machine 1, the cylinder body of the lifting cylinder 28 is fixed to the output end of the rotating cylinder 27, the liquid aspirator 31 is fixedly connected to the output end of the lifting cylinder 28 via the connecting frame 29, and the stain storage tank 30 and the centrifugal test tube 4 are arranged on the rotation path of the liquid aspirator 31. After the cells are centrifuged on the centrifugal preparation mechanism, they can be stained by the staining mechanism. During staining, the rotating cylinder 27 controls the horizontal position of the liquid aspirator 31, allowing the liquid aspirator 31 to move back and forth between the stain storage tank 30 and the centrifugal test tube 4. The lifting cylinder 28 controls the vertical position of the liquid aspirator 31, reducing the vertical distance between the liquid aspirator 31, the dye storage tank 30, and the centrifuge tube 4. This ensures that the liquid aspirator 31 can accurately aspirate the dye in the dye storage tank 30 and accurately drip the aspirated dye into the centrifuge tube 4. This design not only improves the accuracy and efficiency of the dyeing process, but also reduces operator error, ensuring consistent and uniform dyeing.

[0045] Combine Figure 10 To achieve the absorption and release of dye, in this exemplary embodiment, the liquid suction device 31 includes a housing 310, a rubber bladder 311, a suction cylinder 312, and a liquid delivery tube 313. The housing 310 is fixed to the connecting frame 29, the rubber bladder 311 is disposed within the housing 310, and the suction cylinder 312 is mounted on the top of the housing 310. The output end of the suction cylinder 312 extends into the inner wall of the housing 310 and is fixedly connected to a pressure plate 314. The liquid delivery tube 313 is disposed at the bottom of the housing 310 and communicates with the rubber bladder 311. With this arrangement, when the liquid suction device 31 moves above the dye storage tank 30, the suction cylinder 312 first drives the pressure plate 314 downward, compressing the rubber bladder 311. Then, the lifting cylinder 28 controls the liquid suction device 31 to move downward, allowing the liquid delivery tube 313 to be inserted into the dye storage tank 30. Then, the suction cylinder 312 drives the pressure plate 314 to move upward, generating negative pressure in the rubber bladder 311. At this time, the dye is sucked into the rubber bladder 311 by the liquid feeding tube 313, completing the absorption of the dye. Finally, the liquid suction device 31 is moved above the centrifugal test tube 4, and the lifting cylinder 28 drives the pressure plate 314 to move downward, squeezing the dye in the rubber bladder 311 outward. The squeezed dye falls into the centrifugal test tube 4, staining the cells in the centrifugal test tube 4. This design ensures the precise absorption and release of the dye, improving the accuracy and consistency of the dyeing process. In addition, by controlling the actions of the suction cylinder 312 and the lifting cylinder 28, automated operation can be achieved, reducing human intervention and further improving the efficiency and quality of dyeing.

[0046] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. A cell slide preparation and staining machine, comprising a machine platform (1) and a centrifugal slide preparation mechanism and a staining mechanism arranged on the machine platform (1), characterized in that: The centrifugal film making mechanism comprises a centrifugal disc (3) driven to rotate by a driving device (2) and a centrifugal test tube (4) rotatably arranged on the centrifugal disc (3); the centrifugal test tube (4) rotates around a rotation axis X along a first rotation direction when the centrifugal disc (3) rotates; a separation device is arranged between the centrifugal test tube (4) and the centrifugal disc (3); the separation device is constructed to be able to apply a force along the first rotation direction to the bottom of the centrifugal test tube (4) when the centrifugal force applied to the centrifugal test tube (4) ends; The centrifugal film making mechanism further comprises a sleeve (5), the sleeve (5) being rotatably arranged on the centrifugal disk (3) around a rotation axis X, one end of the sleeve (5) being provided with a first slot (6), the other end of the sleeve (5) being provided with a second slot (7) connected to the first slot (6), the centrifugal test tube (4) being a tubular structure with openings at both ends and being pluggable and arranged in the first slot (6), and a glass slide (8) capable of covering the tube opening of the centrifugal test tube (4) being pluggable and arranged in the second slot (7); The centrifugal tablet making mechanism further comprises a U-shaped bracket (10), the bottom of the bracket (10) being fixed to the centrifugal disc (3), the two opposite sides of the sleeve (5) being provided with columnar connecting shafts (11), the two inner side walls of the bracket (10) being provided with obliquely arranged connecting grooves (12), the connecting grooves (12) forming an opening on the end surface of the bracket (10), and the sleeve (5) being movably connected to the connecting grooves (12) via the connecting shafts (11); The de-fixing device comprises a traction frame (13), the traction frame (13) being rotatably arranged on the bracket (10) around a rotation axis Y, and a first torsion spring (14) being arranged at a rotational connection position between the two, and the free end of the traction frame (13) being able to be magnetically fixed or clamped to the sleeve (5) after the centrifugal test tube (4) is rotated by a predetermined angle along a first rotation direction.

2. The integrated cell preparation and staining machine according to claim 1, characterized in that: A clearance groove (9) is provided at the communicating position between the first slot (6) and the second slot (7), and the clearance groove (9) shares an opening with the second slot (7).

3. The integrated cell preparation and staining machine according to claim 1, characterized in that: A permanent magnet (15) is fixedly provided at the free end of the traction frame (13), an iron piece (16) is fixedly provided on the outer wall of the sleeve (5), and the permanent magnet (15) is magnetically fixed to the iron piece (16) after the centrifugal test tube (4) rotates by a predetermined angle in the first rotation direction.

4. The integrated cell preparation and staining machine according to claim 1, characterized in that: A clamping frame is fixedly arranged on the side wall of the sleeve (5), and the clamping frame comprises a fixing portion (17) and a folding portion (18). A first end of the fixing portion (17) is fixedly connected to the outer wall of the sleeve (5), and the folding portion (18) is rotatably connected to a second end of the fixing portion (17) via a rotating shaft (19). A protrusion (20) for limiting the rotation angle of the folding portion (18) is arranged at the second end of the fixing portion (17), and a second torsion spring (21) is arranged at a rotation connection position between the fixing portion (17) and the folding portion (18).

5. The integrated cell preparation and staining machine according to any one of claims 3 or 4, characterized in that: The centrifugal film making mechanism further comprises a waste liquid tank (22), wherein the waste liquid tank (22) is mounted on the centrifugal disk (3), and waste liquid in the centrifugal test tube (4) can be discharged into the waste liquid tank (22) after the centrifugal test tube (4) is rotated by a predetermined angle along a first rotation direction.

6. The integrated cell preparation and staining machine according to any one of claims 3 or 4, characterized in that: The dyeing mechanism comprises a rotating cylinder (27), a lifting cylinder (28), a connecting frame (29), a liquid suction device (31) and a dye storage tank (30); the cylinder body of the rotating cylinder (27) is fixedly mounted on the machine platform (1); the cylinder body of the lifting cylinder (28) is fixed to the output end of the rotating cylinder (27); the liquid suction device (31) is fixedly connected to the output end of the lifting cylinder (28) via the connecting frame (29); and the dye storage tank (30) and the centrifugal test tube (4) are arranged on a rotating path of the liquid suction device (31).

7. The integrated cell preparation and staining machine according to claim 6, characterized in that: The liquid suction device (31) comprises a housing (310), a rubber bag (311), a suction cylinder (312) and a liquid delivery pipe (313); the housing (310) is fixed to the connecting frame (29); the rubber bag (311) is arranged in the housing (310); the suction cylinder (312) is installed on the top of the housing (310); the output end of the suction cylinder (312) extends into the inner wall of the housing (310) and is fixedly connected to a pressure plate (314); and the liquid delivery pipe (313) is arranged at the bottom of the housing (310) and is in communication with the rubber bag (311).

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