A multi-dimensionally adjustable head fixator for awake mice

Abstract

The present invention provides a multi-dimensionally adjustable head fixator for awake mice, comprising a first adjustment component, a second adjustment component, and a window patch. The first adjustment component is mounted on a chassis and has a displacement degree of freedom in a first direction and a rotational degree of freedom in a second direction relative to the chassis; the second adjustment component is mounted on the first adjustment component and has a rotational degree of freedom in a first direction and a rotational degree of freedom in a third direction relative to the chassis; the window patch is mounted on the second adjustment component and has a displacement degree of freedom within a plane formed by the second and third directions relative to the chassis. The present invention, through the provision of the first adjustment component, the second adjustment component, and the window patch, achieves fixation of the animal's imaging site and a multi-dimensionally adjustable fixator for imaging, without completely restraining the animal's own activities, thereby solving the problem of fixing the head of awake mice and matching the needs of behavioral experiments.

Description

Technical Field

[0001] The invention relates to the technical field of animal experimental instruments, in particular to a multi-dimensionally adjustable head fixer for awake mice. Background Art

[0002] The brain plays a vital role in life and is the most complex organ in living organisms. Therefore, studying brain function plays a crucial role in neurobiology. Currently, neurobiological research often uses animal models, such as mice and macaques. To study brain function in experimental animals, microscopic imaging of blood vessels or neurons within the animals' brains is often performed.

[0003] In microscopic imaging, two-photon microscopy, with its advantages of deep penetration and low scattering, allowing for deep-layer imaging, has become widely used in functional imaging of living animals. In functional imaging of living animals, awake mice can respond behaviorally, demonstrating realistic physiological responses, making it easier to conduct physiological studies of relevant neurons. Therefore, a device that can be used to fix the head of awake mice and match behavioral imaging is crucial.

[0004] There are existing instruments for head fixation in awake mice that can precisely adjust the angles in both the AP (anteroposterior bregma) and ML (anteroposterior raphe) axes, with a range of greater than 15 degrees in each axis. However, in practice, the mouse's body is completely immobilized within the restraint, making it unsuitable for behavioral experiments. Furthermore, the instrument design is complex and expensive.

[0005] There is also a head fixator for awake mice used in experiments. It adds a head adjustment device to the restraint tube, but the mouse's body is still completely in the restraint tube. In actual use, the head adjustment device will also conflict with the objective lens space during imaging.

[0006] It should be noted that the information disclosed in the above background technology section is only used to enhance the understanding of the background of the present invention and does not constitute any limitation to the present invention. Summary of the Invention

[0007] In view of the shortcomings of the prior art described above, the present invention provides a multi-dimensionally adjustable head fixator for awake mice. By providing a first adjustment component, a second adjustment component, and a window patch, the fixator can fix the part of the animal to be imaged and can be multi-dimensionally adjusted to cooperate with the imaging without completely restraining the animal's own activities, thereby solving the problem of fixing the head of awake mice and matching the needs of behavioral experiments.

[0008] The present invention provides a multi-dimensionally adjustable head fixation device for awake mice, comprising:

[0009] a first adjustment assembly, the first adjustment assembly being mounted on the chassis and having a displacement degree of freedom in a first direction and a rotation degree of freedom in a second direction relative to the chassis;

[0010] a second adjustment assembly, the second adjustment assembly being mounted on the first adjustment assembly and having a rotational freedom in a first direction and a rotational freedom in a third direction relative to the chassis; and

[0011] The window patch is mounted on the second adjustment component and has the freedom of displacement within the plane formed by the second direction and the third direction relative to the chassis.

[0012] In one embodiment of the present invention, a step is provided on the surface of the chassis on which the first adjustment component is installed. The step is provided along the contour of the chassis, and a gap is left between the step and the edge of the chassis surface to form an outer contour plane at the edge of the chassis.

[0013] In one embodiment of the present invention, the first adjustment component includes a height adjustment frame and a crossbeam. The bottom end of the height adjustment frame is installed on the chassis, and a through slot is opened on its upper part along the first direction; the end of the crossbeam is installed on the through slot through a connecting piece, and the crossbeam can rotate along the second direction.

[0014] In one embodiment of the present invention, the height adjustment frame is further provided with angle scale lines, and the angle scale lines are used to measure the rotation angle of the beam in the through slot in the second direction.

[0015] In one embodiment of the present invention, a through wedge-shaped groove is formed on the crossbeam along the first direction, and a through screw hole is formed in the wedge-shaped groove along the third direction.

[0016] In one embodiment of the present invention, the second component includes an adapter plate and a fixed plate. The adapter plate is installed on the beam through a connecting member, and the adapter plate can rotate along the third direction; the fixed plate is installed at the end of the adapter plate through a connecting member, and the fixed plate can rotate along the first direction.

[0017] In one embodiment of the present invention, the second adjustment component includes an adjustment neck, an adapter plate and a fixed plate, the adjustment neck is installed in the wedge-shaped groove; the adapter plate is installed on the adjustment neck through a connecting member, and the adapter plate can rotate along the third direction; the fixed plate is installed on the end of the adapter plate through a connecting member, and the fixed plate can rotate along the first direction.

[0018] In one embodiment of the present invention, a through hole is provided on the adjusting neck, and a connecting hole corresponding to the through hole is provided on the adapter plate. The adjusting neck and the adapter plate are connected by a bolt passing through the through hole and the connecting hole, so that the adapter plate can rotate along the third direction; the adjusting neck is locked and positioned on the beam by a screw passing through the screw hole and abutting against the adjusting neck in the wedge-shaped groove, so that the adjusting neck can move along the first direction.

[0019] In one embodiment of the present invention, a U-shaped groove extending through the first direction is formed on the fixing plate, and a plurality of pairs of positioning holes extending through the first direction are formed on the fixing plate on both sides of the U-shaped groove.

[0020] In one embodiment of the present invention, the window patch includes a central hole and positioning slots located on both sides of the central hole. The central hole is used to expose the imaging observation area, and the window patch is installed on a plurality of pairs of positioning holes through the positioning slots.

[0021] The beneficial effects of the present invention are as follows: by setting up the first adjustment component, the second adjustment component and the window patch, the spatial displacement degree of freedom and the rotational degree of freedom that exist in each are combined to achieve the fixation of the animal's imaging part and the multi-dimensional adjustment and imaging coordination fixation instrument, and the fixation instrument will not completely restrain the animal's own activities, which is conducive to fully observing the activities of the animal subject during the experiment. It is suitable for behavioral experiments and two-photon imaging coordination experiments.

[0022] It is to be understood that the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] The accompanying drawings are incorporated into and constitute a part of the specification, illustrate embodiments consistent with the present invention, and together with the specification, are used to explain the principles of the present invention. Obviously, the drawings described below are only some embodiments of the present invention, and it is clear that a person skilled in the art can derive other drawings based on these drawings without inventive effort. In the drawings:

[0024] Figure 1 A schematic structural diagram of the multi-dimensionally adjustable fixing device of the present invention at a first viewing angle;

[0025] Figure 2 Schematic diagram of the structure of the multi-dimensionally adjustable fixing device of the present invention at a second viewing angle;

[0026] Figure 3 Schematic diagram of the structure of the chassis in the present invention;

[0027] Figure 4 Schematic diagram of the structure of the height adjustment frame of the present invention;

[0028] Figure 5 Schematic diagram of the structure of the crossbeam in the present invention;

[0029] Figure 6 This is an exploded schematic diagram of the second adjustment component structure in the present invention;

[0030] Figure 7 Schematic diagram of the explosion of the fixing plate and the window patch structure of the present invention;

[0031] Figure 8 Schematic diagram of the structure of the window patch in the present invention.

[0032] In the figure: 10, chassis; 101, screw hole; 102, step; 103, outer contour plane; 1, first adjustment component; 11, height adjustment frame; 110, round hole; 111, through slot; 112, angle scale line; 12, crossbeam; 120, threaded hole; 121, wedge-shaped slot; 122, screw hole; 2, second adjustment component; 21, adjustment neck; 210, through hole; 22, adapter plate; 220, connecting hole; 23, fixing plate; 230, through hole; 231, U-shaped slot; 232, positioning hole; 3, window patch; 31, center hole; 32, positioning slot. DETAILED DESCRIPTION

[0033] The following describes the embodiments of the present invention by means of specific examples. Those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification. The present invention can also be implemented or applied through other different specific embodiments. The details in this specification can also be modified or changed based on different viewpoints and applications without departing from the spirit of the present invention. It should be noted that the features in the following embodiments and examples can be combined with each other unless they conflict. It should also be understood that the terms used in the embodiments of the present invention are intended to describe specific embodiments, not to limit the scope of protection of the present invention.

[0034] See also Figures 1 to 8 . It should be noted that the structures, proportions, sizes, etc. illustrated in the drawings of this specification are only used to match the contents disclosed in the specification for people familiar with this technology to understand and read, and are not used to limit the limiting conditions for the implementation of the present invention. Therefore, they have no substantive technical significance. Any modification of the structure, change in the proportional relationship, or adjustment of the size should still fall within the scope of the technical content disclosed by the present invention without affecting the efficacy and purpose that can be achieved by the present invention. At the same time, the terms such as position and quantitative relationship quoted in this specification are only for the convenience of description, and are not used to limit the scope of the implementation of the present invention. Changes or adjustments in their relative relationships should also be regarded as the scope of the implementation of the present invention without substantially changing the technical content.

[0035] See also Figure 1 and Figure 2 The present invention provides a multi-dimensionally adjustable head fixation device for awake mice, comprising:

[0036] A first adjustment assembly 1 is mounted on the chassis 10 and has a displacement degree of freedom in a first direction and a rotation degree of freedom in a second direction relative to the chassis 10;

[0037] a second adjustment assembly 2 , which is mounted on the first adjustment assembly 1 and has a rotational freedom in a first direction and a rotational freedom in a third direction relative to the chassis 10 ; and

[0038] The window patch 3 is mounted on the second adjustment component 2 and has the freedom of displacement relative to the chassis 10 within the plane formed by the second direction and the third direction.

[0039] Specifically, in an embodiment of the present invention, chassis 10 serves as the base of the fixture, providing stable support and being fixed to a laboratory table. A first adjustment component 1 is mounted on chassis 10 and provides corresponding spatial degrees of freedom. Furthermore, a second adjustment component 2 is mounted on first adjustment component 1, also providing new spatial degrees of freedom. Finally, a window patch 3 is mounted on second adjustment component 2, also providing corresponding spatial degrees of freedom. In this way, the spatial degrees of freedom formed by the three structures are coordinated to secure the animal body part to be imaged in the experiment and provide multi-dimensional adjustment capabilities.

[0040] More specifically, the first adjustment component 1 provides a displacement degree of freedom in a first direction and a rotational degree of freedom in a second direction relative to the chassis 10. For example, a slide rail device can be used to perform translational adjustment along the vertical axis (i.e., the first direction) of the chassis 10, or the chassis 10 can be connected by a rotation axis to allow the first adjustment component 1 to rotate around a horizontal axis (i.e., the second direction). The second adjustment component 2 provides a rotational degree of freedom in a first direction and a rotational degree of freedom in a third direction relative to the chassis 10. For example, it can be made to rotate around a vertical axis (first direction) relative to the chassis 10, and the component can simultaneously rotate around an axis perpendicular to the first direction (third direction). The window patch 3 can be made of titanium alloy and bonded to an animal experimental subject such as a mouse skull using dental cement. It is light in weight, has good hardness, and is suitable for long-term experiments. In the structure of the window patch 3, a slide rail or a plurality of positioning points can be used to achieve displacement movement so as to perform displacement within the plane formed by the second direction and the third direction (the horizontal plane relative to the chassis 10).

[0041] It should be noted that the different components in the first adjustment component 1, the second adjustment component 2 and the window patch 3, such as sliding connection methods such as slide rails, are equipped with rotational connection methods such as screw locking mechanisms to maintain the desired angle, so that relative displacement or rotational freedom is formed between the components, which will not be elaborated here.

[0042] In this way, by setting up the first adjustment component 1, the second adjustment component 2 and the window patch 3, through the combination of their respective spatial displacement degrees of freedom and rotational degrees of freedom, the head of the experimental object such as a mouse can be stably fixed, and the fixation of the animal's imaging part and the multi-dimensional adjustment and imaging fixation device can be achieved without completely restraining the animal's own activities, which is conducive to fully observing the activities of the animal object during the experiment and is suitable for the coordination of behavioral experiments and two-photon imaging.

[0043] See also Figure 1 and Figure 3 In one embodiment, a step 102 is provided on the surface of the chassis 10 on which the first adjustment component 1 is installed. The step 102 is provided along the contour of the chassis 10 , and a gap is left between the step 102 and the edge of the surface of the chassis 10 to form an outer contour plane 103 at the edge of the chassis 10 .

[0044] Specifically, in this embodiment of the present invention, chassis 10 can be a disc-shaped, circular surface with corresponding screw holes 101 formed therein for attaching and securing first adjustment assembly 1. Steps 102 provided on its surface create a accommodating space on chassis 10, ensuring that excrement from experimental subjects, such as mice, during immobilization does not contaminate the space outside chassis 10, such as a laboratory bench. The resulting outer surface 103 serves as a positioning surface for the fixture, securing the fixture to the bench.

[0045] See also Figure 1 and Figure 2 、 Figure 4 and Figure 5 In one embodiment, the first adjustment component 1 includes a height adjustment frame 11 and a crossbeam 12. The bottom end of the height adjustment frame 11 is mounted on the chassis 10, and a through slot 111 is opened on its upper portion along the first direction; the end of the crossbeam 12 is mounted on the through slot 111 through a connecting piece, so that the crossbeam 12 can rotate along the second direction.

[0046] Specifically, in an embodiment of the present invention, a first adjustment assembly 1 is formed by the structure of a height adjustment frame 11 and a crossbeam 12. The height adjustment frame 11 can be configured as an L-shape, with a circular hole 110 at its bottom corresponding to the screw hole 101 of the chassis 10 for screw connection and fixation. A through slot 111 provided at the top of the height adjustment frame 11 is used to mount the end of the crossbeam 12, allowing the crossbeam 12 to select a specific fixed position within the through slot 111. That is, the crossbeam 12 can be moved up and down along the through slot 111 to adjust its height, thereby forming a degree of freedom of displacement in the first direction. Similarly, a threaded hole 120 is provided at the end of the crossbeam 12 connected to the through slot 111, with the diameter of the threaded hole 120 matching the width of the through slot 111. This allows the crossbeam 120 to be installed and connected to the threaded hole 120 using a thumb screw and locked to the through slot 111. In other words, the connecting member can be represented by the threaded hole 120 provided in the crossbeam 12 and the thumb screw used. In this way, the position of the beam 12 in the through slot 111 can be adjusted by tightening the thumb screw, and the angle of the beam 12 can be adjusted during the process of tightening the thumb screw, that is, a rotational degree of freedom in the second direction is formed, so that the axial rotation along the thumb screw can be used to adjust the angle of the experimental object such as the AP (front to back end) of the mouse.

[0047] See also Figure 4 The height adjustment frame 11 is also provided with angle scale lines 112, which are used to measure the rotation angle of the crossbeam 12 in the second direction in the through slot 111. By providing scale lines at corresponding positions on the height adjustment frame 11, for example, by posting or carving angle scale lines 112 on one side of both ends of the through slot 111, the corresponding angles of the crossbeam 12 can be indicated to facilitate intuitive operation.

[0048] See also Figure 5 The crossbeam 12 is provided with a wedge-shaped slot 121 extending in the first direction, and a screw hole 122 extending in the third direction is provided in the wedge-shaped slot 121. The wedge-shaped slot 121 and the screw hole 122 therein can be abutted against a corresponding component of the second adjustment assembly 2, such as the adjustment neck 21, via a hand screw installed in the screw hole 122, to provide the second adjustment assembly 2 with freedom of movement in the first direction relative to the crossbeam 12.

[0049] In one embodiment, the second component includes an adapter plate 22 and a fixed plate 23. The adapter plate 22 is installed on the beam 12 through a connecting member, and the adapter plate 22 can rotate along the third direction; the fixed plate 23 is installed at the end of the adapter plate 22 through a connecting member, and the fixed plate 23 can rotate along the first direction.

[0050] Specifically, in the embodiment of the present invention, the second adjustment assembly 2 (not shown in the drawings) can be directly formed by the adapter plate 22 and the fixed plate 23. That is, the wedge-shaped groove 121 and the corresponding adjustment neck 21 structure can be omitted from the crossbeam 12, and the second adjustment assembly 2 can still provide two degrees of rotational freedom. That is, by providing corresponding through holes 210 and connecting holes 220 in the third direction and the first direction respectively corresponding to the adapter plate 22 and the fixed plate 23, and connecting them with corresponding thumb screws (that is, the connecting members can be implemented using locking bolts or thumb screws according to the fixed or movable requirements of the relevant components, which will not be repeated in the embodiment of the present invention), rotational adjustment in the corresponding directions can be achieved.

[0051] See also Figure 6 In one embodiment, the second adjustment component 2 includes an adjusting neck 21, an adapter plate 22 and a fixed plate 23. The adjusting neck 21 is installed in the wedge-shaped groove 121; the adapter plate 22 is installed on the adjusting neck 21 through a connecting piece, and the adapter plate 22 can rotate along the third direction; the fixed plate 23 is installed on the end of the adapter plate 22 through a connecting piece, and the fixed plate 23 can rotate along the first direction.

[0052] Furthermore, a through hole 210 is provided on the adjusting neck 21, and a connecting hole 220 corresponding to the through hole 210 is provided on the adapter plate 22. The adjusting neck 21 and the adapter plate 22 are connected by bolts passing through the through hole 210 and the connecting hole 220, so that the adapter plate 22 can rotate along the third direction; the adjusting neck 21 is locked and positioned on the beam 12 by a screw passing through the screw hole 122 and abutting against the adjusting neck 21 in the wedge-shaped groove 121, so that the adjusting neck 21 can move along the first direction.

[0053] Specifically, in an embodiment of the present invention, the second adjustment assembly 2 is composed of an adjustment neck 21, an adapter plate 22, and a fixed plate 23. The adjustment neck 21 is matched and fixed with the wedge-shaped groove 121 in the crossbeam 12. The through hole 210 provided in the adjustment neck 21 is used to match and fix with the connecting hole 220 and the nut groove in the adapter plate 22. For example, a screw can be passed through the corresponding through hole 210, the connecting hole 220, and the nut groove, and a nut can be installed in the nut groove to complete the connection. The adapter plate 22 can be rotated along the fixed axis of the screw (i.e., the third direction) to adjust the angle of the mouse ML (inside to outside). After adjustment, it can be locked by the corresponding screw and nut structure.

[0054] Similarly, the wedge-shaped slot 121 and the screw hole 122 therein can be used to engage a hand screw installed in the screw hole 122 against the adjustment neck 21 in the wedge-shaped slot 121, thereby meeting the displacement requirement of the second adjustment component 2 in the first direction. At the same time, to meet the rotation requirement of the fixing plate 23 in the first direction, corresponding through holes 230 along the first direction can be formed in the adapter plate 22 and the fixing plate 23, and corresponding connecting parts such as screws and nuts can be used to adjust the orientation of the entire body of the mouse. After adjustment, the screw and nut structure can be used to lock it.

[0055] See also Figure 7 In one embodiment, a U-shaped groove 231 extending through the first direction is formed on the fixing plate 23 , and a plurality of pairs of positioning holes 232 extending through the first direction are formed on the fixing plate 23 on both sides of the U-shaped groove 231 .

[0056] Specifically, in this embodiment of the present invention, the fixing plate 23 is used to mount the window patch 3. The window patch 3 is directly mounted on an animal body part, such as a mouse's head, with a U-shaped groove 231 provided to provide space for movement. Several pairs of positioning holes 232 are provided on the fixing plate 23 on either side of the U-shaped groove 231 to selectively secure the experimental animal subject, such as a mouse, with the window patch 3 attached, at a specific pair of positioning holes 232 as needed.

[0057] See also Figure 8 In one embodiment, the window patch 3 includes a center hole 31 and positioning grooves 32 located on both sides of the center hole 31. The center hole 31 is used to expose the imaging observation part, and the window patch 3 is installed on several pairs of positioning holes 232 through the positioning grooves 32.

[0058] Specifically, the central hole 31 of the window patch 3 allows the cranial window of an animal subject, such as a mouse, to be exposed without affecting subsequent imaging observation experiments. Positioning slots 32 on either side of the window patch 3 allow bolts to secure the window patch 3 to the positioning holes 232 of the fixing plate 23. Furthermore, the lengths of the two positioning slots 32 can be selected to correspond to the left and right locations of the brain region of the animal body, such as the mouse head, where the window is to be opened.

[0059] It should be noted that the fixing plate 23 has the rotational freedom in the first direction, and the multiple pairs of positioning holes 232 thereon realize the displacement freedom of the window patch 3 to be installed and fixed in one direction, and the size difference between the body shape of the animal experimental subject and the width of the U-shaped groove 231, which enables the displacement freedom of the window patch 3 in another direction, that is, the displacement freedom of the window patch 3 in the plane formed by the second direction and the third direction (relative to the horizontal plane of the chassis 10) is realized.

[0060] In one embodiment, the steps for using the fixing device may include the following:

[0061] Before use, the experimental mouse undergoes a craniotomy under anesthesia to expose the tissue to be imaged. For deep brain regions, a prism or grin lens is embedded. Once embedded, a glass patch is applied to the tissue and sealed to the skull with tissue glue. Dental cement is then applied around the cranial window glass, bonding the window patch 3 to the mouse skull, with the glass patch positioned at the center hole 31.

[0062] The mouse that recovers normally after the operation is fixed on the fixator in the embodiment of the present invention, the window patch 3 of mouse head is fixed on the fixed plate 23 with two screws, select suitable fixed distance according to experimental needs, mainly according to the space needed for object lens during animal size and imaging.Move up and down along through groove 111 and regulate neck 21 and move up and down along wedge-shaped groove 121 and regulate the height of mouse by crossbeam 12, make the limbs of mouse contact chassis 10 and freely move.When carrying out living body two-photon imaging, guarantee that mouse cranial window is vertical with object lens light, then need to adjust the front and back left and right angles of mouse in some limbic brain areas, crossbeam 12 can be used for regulating the angle of mouse AP (front end to rear end) along fixed axis rotation, and adapter plate 22 can be used for regulating the angle of mouse ML (inboard to outside) along fixed axis rotation.In addition, fixed plate 23 can be used for regulating the whole body of mouse towards along fixed axis rotation, can more easily match the device direction of behavior like this, as screen device etc., to complete experimentation.

[0063] In summary, the present invention provides a multi-dimensionally adjustable head fixator for awake mice. By setting a first adjustment component, a second adjustment component and a window patch, the head of an experimental object such as a mouse can be stably fixed by utilizing the combination of the existing spatial displacement degrees of freedom and rotational degrees of freedom. This achieves fixation of the animal's imaging part and multi-dimensional adjustment to coordinate imaging. The fixator will not completely restrain the animal's own activities, which is conducive to fully observing the activities of the animal subject during the experiment. The fixator is suitable for coordinated experiments of behavioral experiments and two-photon imaging.

[0064] The above embodiments are merely illustrative of the principles and effects of the present invention and are not intended to limit the present invention. Anyone skilled in the art may modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by one of ordinary skill in the art without departing from the spirit and technical principles disclosed herein are intended to be covered by the claims of the present invention.

Claims

1. A multi-dimensionally adjustable head fixation device for awake mice, characterized in that: include: A first adjustment component (1), the first adjustment component (1) being mounted on the chassis (10) and having a displacement degree of freedom in a first direction and a rotation degree of freedom in a second direction relative to the chassis (10); a second adjustment component (2), the second adjustment component (2) being mounted on the first adjustment component (1) and having a rotational freedom in a first direction and a rotational freedom in a third direction relative to the chassis (10); as well as A window patch (3), the window patch (3) being mounted on the second adjustment component (2) and having a degree of freedom of displacement within a plane formed by a second direction and a third direction relative to the chassis (10); wherein the first direction is a vertical axis of the chassis (10), and the second direction and the third direction form a horizontal plane relative to the chassis (10); The first adjustment component (1) comprises: A height adjustment frame (11), the bottom end of the height adjustment frame (11) is mounted on the chassis (10), and a through slot (111) is formed on the upper portion thereof along a first direction; and A crossbeam (12), the end of which is mounted on the through slot (111) via a connecting piece, and enables the crossbeam (12) to rotate in a second direction; The second adjustment component (2) comprises: an adapter plate (22), the adapter plate (22) being mounted on the crossbeam (12) via a connecting piece, and enabling the adapter plate (22) to rotate along a third direction; and A fixed plate (23) is mounted on the end of the adapter plate (22) through a connecting piece, and enables the fixed plate (23) to rotate along a first direction.

2. A multi-dimensionally adjustable head fixation device for awake mice, characterized in that: include: A first adjustment component (1), the first adjustment component (1) being mounted on the chassis (10) and having a displacement degree of freedom in a first direction and a rotation degree of freedom in a second direction relative to the chassis (10); a second adjustment component (2), the second adjustment component (2) being mounted on the first adjustment component (1) and having a rotational freedom in a first direction and a rotational freedom in a third direction relative to the chassis (10); as well as A window patch (3), the window patch (3) being mounted on the second adjustment component (2) and having a degree of freedom of displacement within a plane formed by a second direction and a third direction relative to the chassis (10); wherein the first direction is a vertical axis of the chassis (10), and the second direction and the third direction form a horizontal plane relative to the chassis (10); The first adjustment component (1) comprises: A height adjustment frame (11), the bottom end of the height adjustment frame (11) is mounted on the chassis (10), and a through slot (111) is formed on the upper portion thereof along a first direction; and A crossbeam (12), the end of which is mounted on the through slot (111) via a connecting piece, and enables the crossbeam (12) to rotate in a second direction; A through wedge-shaped groove (121) is provided on the crossbeam (12) along a first direction, and a screw hole (122) is provided in the wedge-shaped groove (121) along a third direction; The second adjustment component (2) comprises: an adjusting neck (21), the adjusting neck (21) being installed in the wedge-shaped groove (121); an adapter plate (22), the adapter plate (22) being mounted on the adjusting neck (21) via a connecting piece, and enabling the adapter plate (22) to rotate along a third direction; and A fixed plate (23) is mounted on the end of the adapter plate (22) through a connecting piece, and enables the fixed plate (23) to rotate along a first direction.

3. The fixing device according to claim 1 or 2, characterized in that: A step (102) is provided on the surface of the chassis (10) on which the first adjustment component (1) is mounted. The step (102) is arranged along the contour of the chassis (10), and a gap is left between the step (102) and the edge of the surface of the chassis (10) to form an outer contour plane (103) at the edge of the chassis (10).

4. The fixing device according to claim 1 or 2, characterized in that: An angle scale line (112) is also provided on the height adjustment frame (11), and the angle scale line (112) is used to measure the rotation angle of the crossbeam (12) in the through slot (111) in the second direction.

5. The fixing device according to claim 2, characterized in that: The adjusting neck (21) is provided with a through hole (210), and the adapter plate (22) is provided with a connecting hole (220) corresponding to the through hole (210); a bolt is passed through the through hole (210) and the connecting hole (220) to connect the adjusting neck (21) and the adapter plate (22), so that the adapter plate (22) can rotate along a third direction; a screw is passed through the screw hole (122) to abut against the adjusting neck (21) in the wedge-shaped groove (121), and the adjusting neck (21) is locked and positioned on the crossbeam (12), so that the adjusting neck (21) can move along the first direction.

6. The fixing device according to claim 1 or 2, characterized in that: A U-shaped groove (231) extending through the first direction is provided on the fixing plate (23), and a plurality of pairs of positioning holes (232) extending through the first direction are provided on the fixing plate (23) on both sides of the U-shaped groove (231).

7. The fixing device according to claim 6, characterized in that: The window patch (3) comprises a central hole (31) and positioning grooves (32) located on both sides of the central hole (31); the central hole (31) is used to expose an imaging observation area; and the window patch (3) is mounted on a plurality of pairs of positioning holes (232) via the positioning grooves (32).

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