Bionic liposome for simulating intercellular lipid component and preparation method thereof

Abstract

The invention relates to the technical field of skin care products, and particularly discloses a bionic liposome for simulating intercellular lipid components and a preparation method of the bionic liposome. The bionic liposome for simulating the intercellular lipid component comprises the following components in parts by mass: 30-38 parts of a solvent; 35 to 43 parts of a humectant; 1.34 to 1.66 parts of grease; 2.4 to 2.6 parts of a lipid substance; 2.58 to 3.22 parts of an emulsifier; 18 to 26 parts of a skin conditioner; 4.9 to 5.1 parts of composite ceramide; 1 to 3 parts of phytosphingosine; 0.09 to 0.11 part of a sphingomonas fermentation product extract; and the emulsifier is a compound of olive alcohol, polyglycerol-10 stearate, sodium stearoyl glutamate and sodium lauroyl lactylate. The method has the advantage that ceramide is not easy to crystallize and separate out.

Description

Technical Field

[0001] The invention relates to the field of skin care products, in particular to a bionic liposome simulating an intercellular lipid component and a preparation method thereof. Background Art

[0002] The intercellular lipids in the stratum corneum play a vital role. They protect the health of the skin like a guard. If the intercellular lipids are damaged, the rate of water loss through the epidermis will accelerate, and the skin will be prone to dryness and peeling. Once the integrity of the intercellular lipids is destroyed, water can no longer be effectively locked inside the skin, which leads to faster water loss. At the same time, it will also make it easier for external irritants to enter the deep layers of the skin, causing inflammation and irritation.

[0003] Ceramide, cholesterol and fatty acids are important components of the extracellular matrix. They play a key role in maintaining the skin barrier function. Ceramide enhances the skin's ability to retain moisture and helps the skin stay moisturized. Cholesterol enhances the skin's barrier function and prevents water loss. Fatty acids provide nutrition and promote the renewal and repair of skin cells.

[0004] Therefore, supplementing the skin with ceramide is an important means of repairing the skin. However, ceramide is insoluble in neither water nor oil and has a high melting point. When added in high amounts in skin care products, ceramide tends to crystallize and precipitate, which not only destroys the stability of the applied formula, but also prevents the activity of ceramide from being exerted. Therefore, there is still room for improvement. Summary of the invention

[0005] In order to prevent ceramide from crystallizing and precipitating, the present application provides a bionic liposome simulating an intercellular lipid component and a preparation method thereof.

[0006] In a first aspect, the present application provides a biomimetic liposome that simulates intercellular lipid components, using the following technical solution: A biomimetic liposome simulating an intercellular lipid component, comprising the following components in parts by weight: 30-38 parts of solvent; 35-43 parts of moisturizer; Oil 1.34-1.66 parts; Lipids 2.4-2.6 parts; Emulsifier 2.58-3.22 parts; 18-26 parts of skin conditioning agent; Composite ceramide 4.9-5.1 parts; 1-3 parts of phytosphingosine; 0.09-0.11 parts of Sphingomonas fermentation product extract; The emulsifier is a compound of olive oil alcohol, polyglyceryl-10 stearate, sodium stearoyl glutamate and sodium lauroyl lactylate.

[0007] By adopting the above technical scheme, by specifically selecting the combination of olive oil alcohol, polyglyceryl-10 stearate, sodium stearoyl glutamate, and sodium lauroyl lactylate as emulsifiers, the biomimetic liposome system simulating the intercellular lipid component can be stabilized, and ceramide crystallization will not occur after long-term storage, so that the biomimetic liposome simulating the intercellular lipid component can be well absorbed by the skin and can give full play to the effect of ceramide, thereby achieving a better effect of repairing the skin.

[0008] By adding phytosphingosine and Sphingomonas fermentation product extract, phytosphingosine is a ceramide precursor naturally present in the surface layer of the skin, and Sphingomonas fermentation product extract is a microorganism that synthesizes ceramide. These two active ingredients can promote the synthesis of ceramide, thereby allowing liposomes to repair the intercellular lipid components while also supplementing ceramide, thereby improving the skin's repair effect.

[0009] Through the coordination of various components, the bionic liposomes that simulate the intercellular lipid components have excellent moisturizing and repairing effects, solving the problem of crystallization of ceramide in the application of skin care formulas. It has good water solubility and can be added during the stirring and cooling stage of the skin care formula, greatly reducing related production costs. It can be used in products such as sebum repair, moisturizing, anti-allergic and soothing, and is applicable to lotions, creams, gels and essence water formulations.

[0010] Preferably, the mass ratio of the olive oil alcohol, polyglyceryl-10 stearate, sodium stearoyl glutamate, and sodium lauroyl lactylate is 1.9-2.1:0.2-0.4:0.4-0.6:0.08-0.12.

[0011] By adopting the above technical scheme and specifically selecting the mass ratio of olive oil alcohol, polyglyceryl-10 stearate, sodium stearoyl glutamate, and sodium lauroyl lactylate, the stability of ceramide in the bionic liposomes simulating the intercellular lipid components is improved, the precipitation phenomenon is less likely to occur, and the efficacy of ceramide is better exerted.

[0012] Preferably, the composite ceramide is a compound of ceramide EOP, ceramide NS / ceramide NG, ceramide NP, ceramide AS and ceramide AP, and the mass ratio of ceramide EOP, ceramide NS / ceramide NG, ceramide NP, ceramide AS and ceramide AP is 0.11-0.15: 0.85-0.89: 2.63-2.67: 0.33-0.37: 0.98-1.02.

[0013] By adopting the above technical scheme, the effect of repairing skin is more significant by specifically selecting the combination of ceramide EOP, ceramide NS / ceramide NG, ceramide NP, ceramide AS and ceramide AP.

[0014] Preferably, the solvent is a compound of deionized water, decanol, hexyldecanol and decanediol, and the mass ratio of the deionized water, decanol, hexyldecanol and decanediol is 8-10:4-6:14-16:4-6.

[0015] By adopting the above technical solution, the solubility of each component is improved by specifically selecting the compound of ionized water, decanol, hexyldecanol and decanediol, so that the product stability is higher.

[0016] Preferably, the moisturizing agent is a compound of panthenol, lactitol, phytol and octanediol, and the mass ratio of the panthenol, lactitol, phytol and octanediol is 13-15:4-6:9-11:9-11.

[0017] By adopting the above technical solution and specifically selecting the combination of panthenol, lactitol, phytol and caprylyl glycol, the skin's ability to retain water and retain moisture can be improved, the evaporation of moisture in the skin can be prevented, and while moisturizing the skin, microcirculation can be improved and the skin's moisturizing degree can be enhanced.

[0018] Preferably, the oil is a compound of meadowfoam seed oil, peony seed oil, grape seed oil and sunflower seed oil, and the mass ratio of meadowfoam seed oil, peony seed oil, grape seed oil and sunflower seed oil is 1-1.2:0.18-0.22:0.08-0.12:0.08-0.12.

[0019] By adopting the above technical scheme and specifically selecting the combination of meadowfoam seed oil, peony seed oil, grape seed oil and sunflower seed oil, the skin can be better repaired, nutrients can be supplemented for the skin, and the skin can be healthier.

[0020] Preferably, the lipid substance is cholesterol.

[0021] By adopting the above technical solution and specifically selecting cholesterol, the skin can be repaired better and recover faster.

[0022] Preferably, the skin conditioner is a compound of hydrogenated soybean oil, oxidized corn oil, hydrogenated sunflower oil and egg yolk oil, and the mass ratio of the hydrogenated soybean oil, oxidized corn oil, hydrogenated sunflower oil and egg yolk oil is 9-11:4-6:4-6:1-3.

[0023] By adopting the above technical solution and specifically selecting a combination of hydrogenated soybean oil, oxidized corn oil, hydrogenated sunflower oil, and egg yolk oil, it is possible to assist in improving the metabolic activity of cells and achieve a rapid repair effect.

[0024] In a second aspect, the present application provides a method for preparing a biomimetic liposome that simulates an intercellular lipid component, using the following technical solution: A method for preparing the above-mentioned biomimetic liposome simulating intercellular lipid components comprises the following steps: Step 1), mixing the composite ceramide, oil and lipid substances at 90-95° C. to obtain a first dispersed phase; Step 2), the solvent and the moisturizer are mixed at 80-85° C. to obtain a first mixture, the emulsifier, the skin conditioner, and the phytosphingosine are mixed at 85-90° C. to obtain a second mixture, and the first mixture and the second mixture are mixed to obtain a second dispersed phase; Step 3), mixing the first dispersed phase and the second dispersed phase, and homogenizing to obtain a biomimetic lipid mixture; Step 4), cooling the biomimetic lipid mixture to ≤45°C, adding the extract of the fermentation product of Sphingomonas, and mixing evenly to obtain biomimetic liposomes simulating the intercellular lipid components.

[0025] By adopting the above technical scheme, the bionic liposomes simulating the intercellular lipid components are of better quality and have excellent moisturizing and repairing effects. The problem of crystallization of ceramide in the application of skin care formulas is solved. The ceramides have good water solubility and can be added during the stirring and cooling stage of the skin care formula, which greatly reduces the relevant production costs. The liposomes can be applied to sebum repair, moisturizing, anti-allergic and soothing products, and are applicable to lotions, creams, gels and essence water formulations.

[0026] In summary, this application has the following beneficial effects: 1. Since the present application specifically selects olive oil alcohol, polyglycerol-10 stearate, sodium stearoyl glutamate, and sodium lauroyl lactylate as emulsifiers, the bionic liposome system simulating the intercellular lipid component can be stabilized, and ceramide crystallization will not occur after long-term storage, so that the bionic liposome simulating the intercellular lipid component can be well absorbed by the skin, and the effect of ceramide can be fully exerted, so that the effect of repairing the skin is better.

[0027] 2. In the present application, it is preferred to add phytosphingosine and Sphingomonas fermentation product extract. Phytosphingosine is a ceramide precursor naturally present in the surface layer of the skin, and Sphingomonas fermentation product extract is a microorganism that synthesizes ceramide. These two active substances can promote the synthesis of ceramide, thereby allowing liposomes to repair intercellular lipid components while also supplementing ceramide, thereby improving the skin repair effect.

[0028] 3. In the present application, it is preferred to specifically select the mass ratio of olive oil alcohol, polyglyceryl-10 stearate, sodium stearoyl glutamate, and sodium lauroyl lactylate to improve the stability of ceramide in the bionic liposomes simulating the intercellular lipid components, make it less likely to precipitate, and better exert the efficacy of ceramide. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 This is a picture of the appearance of the biomimetic liposome simulating the intercellular lipid component of Example 1 after being placed for 90 days; Figure 2 This is a picture of the appearance of the biomimetic liposome simulating the intercellular lipid component of Example 2 after being placed for 90 days; Figure 3 This is a picture of the appearance of the biomimetic liposome simulating the intercellular lipid component of Example 3 after being placed for 90 days; Figure 4 This is a picture of the appearance of the biomimetic liposome simulating the intercellular lipid component of Comparative Example 1 after being placed for 90 days; Figure 5 This is a picture of the appearance of the biomimetic liposome simulating the intercellular lipid component of Comparative Example 2 after being placed for 90 days; Figure 6 This is a picture of the appearance of the biomimetic liposome simulating the intercellular lipid component of Comparative Example 3 after being placed for 90 days; Figure 7 This is a picture of the appearance of the biomimetic liposome simulating the intercellular lipid component of Comparative Example 4 after being placed for 90 days; Figure 8 This is a picture of the appearance of the bionic liposome simulating the intercellular lipid component of Comparative Example 5 after being placed for 90 days. DETAILED DESCRIPTION

[0030] The present application is further described in detail below with reference to the embodiments.

[0031] Example 1 A bionic liposome simulating intercellular lipid components comprises the following components: solvent, moisturizer, oil, lipid substance, emulsifier, skin conditioner, composite ceramide, phytosphingosine, and extract of sphingomonas fermentation product.

[0032] The solvent is a compound of deionized water, decanol, hexyldecanol and decanediol.

[0033] Decyl alcohol is commercially available, CAS number: 112-30-1.

[0034] Hexyldecanol is commercially available, CAS number: 2425-77-6.

[0035] Decanediol is commercially available, CAS number: 1119-86-4.

[0036] The moisturizer is a combination of panthenol, lactitol, phytol and caprylyl glycol.

[0037] Panthenol is commercially available, CAS number: 81-13-0.

[0038] Lactitol is commercially available, CAS number: 585-86-4.

[0039] Phytol is commercially available, CAS number: 150-86-7.

[0040] Octanediol is commercially available, CAS number: 629-41-4.

[0041] The oil is a compound of meadowfoam seed oil, peony seed oil, grape seed oil and sunflower seed oil.

[0042] Meadowfoam seed oil was purchased from Shaanxi Bocuijian Biotechnology Co., Ltd.

[0043] Peony seed oil was purchased from Shaanxi Bolin Biotechnology Co., Ltd.

[0044] Grape seed oil was purchased from Ji'an Zhongda Natural Plant Flavor Co., Ltd.

[0045] Sunflower seed oil was purchased from Guangzhou Puyifeng Biotechnology Co., Ltd.

[0046] The lipid substance was cholesterol, which was purchased from Jiangsu Caiwei Biotechnology Co., Ltd.

[0047] The emulsifier is a combination of olive oil alcohol, polyglyceryl-10 stearate, sodium stearoyl glutamate, and sodium lauroyl lactylate.

[0048] Oleic acid alcohol was purchased from Shandong Guohua Chemical Co., Ltd., CAS number: 143-19-1.

[0049] Polyglycerol-10 stearate was purchased from Zhongshan Dixin Chemical Co., Ltd., CAS No.: 79777-30-3.

[0050] Sodium stearoyl glutamate was purchased from Jiangsu Pulex Biotechnology Co., Ltd., CAS number: 38517-23-6.

[0051] Sodium lauroyl lactylate was purchased from Hubei Xinyuhong Biopharmaceutical Technology Co., Ltd., CAS number: 13557-75-0.

[0052] The skin conditioning agent is a compound of hydrogenated soybean oil, oxidized corn oil, hydrogenated sunflower oil and egg yolk oil.

[0053] Hydrogenated soybean oil is commercially available. CAS number: 8016-70-4.

[0054] Oxidized corn oil was obtained from a commercial source, CAS number: 223705-62-2 Hydrogenated sunflower oil was purchased from Guangzhou Aoyuan Trading Co., Ltd.

[0055] The egg yolk oil was commercially available, CAS number: 8001-17-0.

[0056] Composite ceramide is a combination of ceramide EOP, ceramide NS / ceramide NG, ceramide NP, ceramide AS, and ceramide AP.

[0057] Ceramide EOP was purchased from Wuhan Kamik Technology Co., Ltd.

[0058] Ceramide NS / Ceramide NG was purchased from Shanghai Jiayu Biotechnology Co., Ltd. Ceramide NP was purchased from Shaanxi Aibonyi Biotechnology Co., Ltd.

[0059] Ceramide AS was purchased from Chongqing Zhihe Biotechnology Co., Ltd.

[0060] Ceramide AP was purchased from Wuhan Kamik Technology Co., Ltd.

[0061] Phytosphingosine was purchased from Hubei Jiufenglong Chemical Co., Ltd.

[0062] The Sphingomonas fermentation product extract was purchased from Shanghai Yuyu New Material Technology Co., Ltd.

[0063] The preparation method of the biomimetic liposome simulating the intercellular lipid component comprises the following steps: Step 1), 0.11kg ceramide EOP, 0.85kg ceramide NS / ceramide NG, 2.63kg ceramide NP, 0.33kg ceramide AS, 0.98kg ceramide AP, 1kg white meadowfoam seed oil, 0.18kg peony seed oil, 0.08kg grape seed oil, 0.08kg sunflower seed oil, and 2.4kg cholesterol are put into an emulsifying pot, heated and kept at 90°C, with a speed of 120r / min, stirred for 3min to obtain a premix, and then the premix is ​​placed in an ultrasonic chamber, and ultrasonically treated for 5min at an ultrasonic frequency of 23KHz and an ultrasonic power of 600W to obtain a first dispersed phase.

[0064] Step 2), 8kg of deionized water, 4kg of decanol, 14kg of hexyldecanol, 4kg of decanediol, 13kg of panthenol, 4kg of lactitol, 9kg of phytol, and 9kg of octanediol are put into an emulsifying pot, heated and kept at a constant temperature of 80-85°C, a speed of 120r / min, stirred for 3min, and mixed evenly to obtain a first mixture, 1.9kg of olive oil alcohol, 0.2kg of polyglycerol-10 stearate, 0.4kg of sodium stearoyl glutamate, 0.08kg of sodium lauroyl lactylate, 9kg of hydrogenated soybean oil, 4kg of oxidized corn oil, 4kg of hydrogenated sunflower oil, 1kg of egg yolk oil, and 1kg of phytosphingosine are put into an emulsifying pot, heated and kept at a constant temperature of 85°C, a speed of 120r / min, stirred for 3min, and mixed evenly to obtain a second mixture, the first mixture and the second mixture are put into the same emulsifying pot, 7500r / min, homogenized for 55s, mixed evenly, and a second dispersed phase is obtained.

[0065] Step 3), the first dispersed phase and the second dispersed phase are put into an emulsifying pot, the rotation speed is 120r / min, stirred for 3min, and mixed evenly to obtain a third mixture, and the third mixture is put into a microfluidizer and homogenized twice at 20000psi to obtain a bionic lipid mixture.

[0066] Step 4), put the biomimetic lipid mixture into an emulsifier, cool it to 45°C, add 0.09kg of Sphingomonas fermentation product extract, rotate at 120r / min, stir for 3min, mix evenly, and obtain biomimetic liposomes simulating intercellular lipid components.

[0067] Example 2 A bionic liposome simulating intercellular lipid components comprises the following components: solvent, moisturizer, oil, lipid substance, emulsifier, skin conditioner, composite ceramide, phytosphingosine, and extract of sphingomonas fermentation product.

[0068] The solvent is a compound of deionized water, decanol, hexyldecanol and decanediol.

[0069] Decyl alcohol is commercially available, CAS number: 112-30-1.

[0070] Hexyldecanol is commercially available, CAS number: 2425-77-6.

[0071] Decanediol is commercially available, CAS number: 1119-86-4.

[0072] The moisturizer is a combination of panthenol, lactitol, phytol and caprylyl glycol.

[0073] Panthenol is commercially available, CAS number: 81-13-0.

[0074] Lactitol is commercially available, CAS number: 585-86-4.

[0075] Phytol is commercially available, CAS number: 150-86-7.

[0076] Octanediol is commercially available, CAS number: 629-41-4.

[0077] The oil is a compound of meadowfoam seed oil, peony seed oil, grape seed oil and sunflower seed oil.

[0078] Meadowfoam seed oil was purchased from Shaanxi Bocuijian Biotechnology Co., Ltd.

[0079] Peony seed oil was purchased from Shaanxi Bolin Biotechnology Co., Ltd.

[0080] Grape seed oil was purchased from Ji'an Zhongda Natural Plant Flavor Co., Ltd.

[0081] Sunflower seed oil was purchased from Guangzhou Puyifeng Biotechnology Co., Ltd.

[0082] The lipid substance was cholesterol, which was purchased from Jiangsu Caiwei Biotechnology Co., Ltd.

[0083] The emulsifier is a combination of olive oil alcohol, polyglyceryl-10 stearate, sodium stearoyl glutamate, and sodium lauroyl lactylate.

[0084] Oleic acid alcohol was purchased from Shandong Guohua Chemical Co., Ltd., CAS number: 143-19-1.

[0085] Polyglycerol-10 stearate was purchased from Zhongshan Dixin Chemical Co., Ltd., CAS No.: 79777-30-3.

[0086] Sodium stearoyl glutamate was purchased from Jiangsu Pulex Biotechnology Co., Ltd., CAS number: 38517-23-6.

[0087] Sodium lauroyl lactylate was purchased from Hubei Xinyuhong Biopharmaceutical Technology Co., Ltd., CAS number: 13557-75-0.

[0088] The skin conditioning agent is a compound of hydrogenated soybean oil, oxidized corn oil, hydrogenated sunflower oil and egg yolk oil.

[0089] Hydrogenated soybean oil is commercially available. CAS number: 8016-70-4.

[0090] Oxidized corn oil was obtained from a commercial source, CAS number: 223705-62-2 Hydrogenated sunflower oil was purchased from Guangzhou Aoyuan Trading Co., Ltd.

[0091] The egg yolk oil was commercially available, CAS number: 8001-17-0.

[0092] Composite ceramide is a combination of ceramide EOP, ceramide NS / ceramide NG, ceramide NP, ceramide AS, and ceramide AP.

[0093] Ceramide EOP was purchased from Wuhan Kamik Technology Co., Ltd.

[0094] Ceramide NS / Ceramide NG was purchased from Shanghai Jiayu Biotechnology Co., Ltd. Ceramide NP was purchased from Shaanxi Aibonyi Biotechnology Co., Ltd.

[0095] Ceramide AS was purchased from Chongqing Zhihe Biotechnology Co., Ltd.

[0096] Ceramide AP was purchased from Wuhan Kamik Technology Co., Ltd.

[0097] Phytosphingosine was purchased from Hubei Jiufenglong Chemical Co., Ltd.

[0098] The Sphingomonas fermentation product extract was purchased from Shanghai Yuyu New Material Technology Co., Ltd.

[0099] The preparation method of the biomimetic liposome simulating the intercellular lipid component comprises the following steps: Step 1), 0.13kg ceramide EOP, 0.87kg ceramide NS / ceramide NG, 2.65kg ceramide NP, 0.35kg ceramide AS, 1kg ceramide AP, 1.1kg white meadowfoam seed oil, 0.2kg peony seed oil, 0.1kg grape seed oil, 0.1kg sunflower seed oil, and 2.5kg cholesterol are put into an emulsifying pot, heated and kept at 92.5° C., a speed of 120 r / min, and stirred for 3 minutes to obtain a premix, and then the premix is ​​placed in an ultrasonic chamber, and ultrasonic frequency is 23.5 KHz, ultrasonic power 650 W uninterrupted ultrasonic 6.5 minutes to obtain a first dispersed phase.

[0100] Step 2), 9kg of deionized water, 5kg of decanol, 15kg of hexyldecanol, 5kg of decanediol, 14kg of panthenol, 5kg of lactitol, 10kg of phytol, and 10kg of octanediol are put into an emulsifying pot, heated and kept at a constant temperature of 80-85°C, a speed of 120r / min, stirred for 3min, and mixed evenly to obtain a first mixture, 2kg of olive oil alcohol, 0.3kg of polyglycerol-10 stearate, 0.5kg of sodium stearoyl glutamate, 0.1kg of sodium lauroyl lactylate, 10kg of hydrogenated soybean oil, 5kg of oxidized corn oil, 5kg of hydrogenated sunflower oil, 2kg of egg yolk oil, and 2kg of phytosphingosine are put into an emulsifying pot, heated and kept at a constant temperature of 87.5°C, a speed of 120r / min, stirred for 3min, and mixed evenly to obtain a second mixture, the first mixture and the second mixture are put into the same emulsifying pot, 8250r / min, homogenized for 75s, mixed evenly, and a second dispersed phase is obtained.

[0101] Step 3), the first dispersed phase and the second dispersed phase are put into an emulsifying pot, the rotation speed is 120r / min, stirred for 3min, and mixed evenly to obtain a third mixture, and the third mixture is put into a microfluidizer and homogenized twice at 25000psi to obtain a bionic lipid mixture.

[0102] Step 4), putting the biomimetic lipid mixture into an emulsifier, cooling to 45°C, adding 0.1 kg of Sphingomonas fermentation product extract, rotating at 120 r / min, stirring for 3 min, and mixing evenly to obtain biomimetic liposomes simulating intercellular lipid components.

[0103] Example 3 A bionic liposome simulating intercellular lipid components comprises the following components: solvent, moisturizer, oil, lipid substance, emulsifier, skin conditioner, composite ceramide, phytosphingosine, and extract of sphingomonas fermentation product.

[0104] The solvent is a compound of deionized water, decanol, hexyldecanol and decanediol.

[0105] Decyl alcohol is commercially available, CAS number: 112-30-1.

[0106] Hexyldecanol is commercially available, CAS number: 2425-77-6.

[0107] Decanediol is commercially available, CAS number: 1119-86-4.

[0108] The moisturizer is a combination of panthenol, lactitol, phytol and caprylyl glycol.

[0109] Panthenol is commercially available, CAS number: 81-13-0.

[0110] Lactitol is commercially available, CAS number: 585-86-4.

[0111] Phytol is commercially available, CAS number: 150-86-7.

[0112] Octanediol is commercially available, CAS number: 629-41-4.

[0113] The oil is a compound of meadowfoam seed oil, peony seed oil, grape seed oil and sunflower seed oil.

[0114] Meadowfoam seed oil was purchased from Shaanxi Bocuijian Biotechnology Co., Ltd.

[0115] Peony seed oil was purchased from Shaanxi Bolin Biotechnology Co., Ltd.

[0116] Grape seed oil was purchased from Ji'an Zhongda Natural Plant Flavor Co., Ltd.

[0117] Sunflower seed oil was purchased from Guangzhou Puyifeng Biotechnology Co., Ltd.

[0118] The lipid substance was cholesterol, which was purchased from Jiangsu Caiwei Biotechnology Co., Ltd.

[0119] The emulsifier is a combination of olive oil alcohol, polyglyceryl-10 stearate, sodium stearoyl glutamate, and sodium lauroyl lactylate.

[0120] Oleic acid alcohol was purchased from Shandong Guohua Chemical Co., Ltd., CAS number: 143-19-1.

[0121] Polyglycerol-10 stearate was purchased from Zhongshan Dixin Chemical Co., Ltd., CAS No.: 79777-30-3.

[0122] Sodium stearoyl glutamate was purchased from Jiangsu Pulex Biotechnology Co., Ltd., CAS number: 38517-23-6.

[0123] Sodium lauroyl lactylate was purchased from Hubei Xinyuhong Biopharmaceutical Technology Co., Ltd., CAS number: 13557-75-0.

[0124] The skin conditioning agent is a compound of hydrogenated soybean oil, oxidized corn oil, hydrogenated sunflower oil and egg yolk oil.

[0125] Hydrogenated soybean oil is commercially available. CAS number: 8016-70-4.

[0126] Oxidized corn oil was obtained from a commercial source, CAS number: 223705-62-2 Hydrogenated sunflower oil was purchased from Guangzhou Aoyuan Trading Co., Ltd.

[0127] The egg yolk oil was commercially available, CAS number: 8001-17-0.

[0128] Composite ceramide is a combination of ceramide EOP, ceramide NS / ceramide NG, ceramide NP, ceramide AS, and ceramide AP.

[0129] Ceramide EOP was purchased from Wuhan Kamik Technology Co., Ltd.

[0130] Ceramide NS / Ceramide NG was purchased from Shanghai Jiayu Biotechnology Co., Ltd. Ceramide NP was purchased from Shaanxi Aibonyi Biotechnology Co., Ltd.

[0131] Ceramide AS was purchased from Chongqing Zhihe Biotechnology Co., Ltd.

[0132] Ceramide AP was purchased from Wuhan Kamik Technology Co., Ltd.

[0133] Phytosphingosine was purchased from Hubei Jiufenglong Chemical Co., Ltd.

[0134] The Sphingomonas fermentation product extract was purchased from Shanghai Yuyu New Material Technology Co., Ltd.

[0135] The preparation method of the biomimetic liposome simulating the intercellular lipid component comprises the following steps: Step 1), 0.15kg ceramide EOP, 0.89kg ceramide NS / ceramide NG, 2.67kg ceramide NP, 0.37kg ceramide AS, 1.02kg ceramide AP, 1.2kg white meadowfoam seed oil, 0.22kg peony seed oil, 0.12kg grape seed oil, 0.12kg sunflower seed oil, 2.6kg cholesterol are put into an emulsifying pot, heated and kept at 95°C, the speed is 120r / min, stirred for 3min, and a premix is ​​obtained, and then the premix is ​​placed in an ultrasonic chamber, and ultrasonic frequency is 24KHz, ultrasonic power is 700W, and ultrasonic is continuously performed for 8min to obtain a first dispersed phase.

[0136] Step 2), 10kg of deionized water, 6kg of decanol, 16kg of hexyldecanol, 6kg of decanediol, 15kg of panthenol, 6kg of lactitol, 11kg of phytol, and 11kg of octanediol are put into an emulsifying pot, heated and kept at a constant temperature of 80-85°C, a speed of 120r / min, stirred for 3min, and mixed evenly to obtain a first mixture, 2.1kg of olive oil alcohol, 0.4kg of polyglycerol-10 stearate, 0.6kg of sodium stearoyl glutamate, 0.12kg of sodium lauroyl lactylate, 11kg of hydrogenated soybean oil, 6kg of oxidized corn oil, 6kg of hydrogenated sunflower oil, 3kg of egg yolk oil, and 3kg of phytosphingosine are put into an emulsifying pot, heated and kept at a constant temperature of 90°C, a speed of 120r / min, stirred for 3min, and mixed evenly to obtain a second mixture, the first mixture and the second mixture are put into the same emulsifying pot, 9000r / min, homogenized for 95s, mixed evenly, and a second dispersed phase is obtained.

[0137] Step 3), the first dispersed phase and the second dispersed phase are put into an emulsifying pot, the rotation speed is 120r / min, stirred for 3min, and mixed evenly to obtain a third mixture, and the third mixture is put into a microfluidizer and homogenized twice at 30000psi to obtain a bionic lipid mixture.

[0138] Step 4), put the biomimetic lipid mixture into an emulsifier, cool it to 45°C, add 0.11 kg of Sphingomonas fermentation product extract, rotate at 120 r / min, stir for 3 min, mix evenly, and obtain biomimetic liposomes simulating intercellular lipid components.

[0139] Comparative Example 1 A biomimetic liposome simulating an intercellular lipid component, compared with Example 2, differs only in that: In the emulsifier, cetearyl glucoside is used to replace olive oil alcohol in an equal amount.

[0140] Cetearyl glucoside was purchased from Hubei Shineng Chemical Technology Co., Ltd., CAS No.: 246159-33-1.

[0141] Comparative Example 2 A biomimetic liposome simulating an intercellular lipid component, compared with Example 2, differs only in that: In the emulsifier, polyglyceryl-10 oleate was used to replace polyglyceryl-10 stearate in an equal amount.

[0142] Polyglycerol-10 oleate was purchased from Jiangxi Huayuyuan Biotechnology Co., Ltd., CAS No.: 9007-48-1.

[0143] Comparative Example 3 A biomimetic liposome simulating an intercellular lipid component, compared with Example 2, differs only in that: In the emulsifier, an equal amount of hydrogenated lecithin is used to replace sodium stearoyl glutamate.

[0144] Hydrogenated lecithin was purchased from Xi'an Kangnuo Chemical Co., Ltd., CAS No.: 92128-87-5.

[0145] Comparative Example 4 A biomimetic liposome simulating an intercellular lipid component, compared with Example 2, differs only in that: In the emulsifier, sorbitan olivate was used to replace sodium lauroyl lactylate in an equal amount.

[0146] Sorbitan olivate was purchased from Wuhan Kemik Biopharmaceutical Technology Co., Ltd., CAS No.: 92128-87-5.

[0147] Comparative Example 5 A biomimetic liposome simulating an intercellular lipid component, compared with Example 2, differs only in that: Among the emulsifiers, cetearyl glucoside is used to replace olive oil alcohol in an equal amount, polyglyceryl-10 oleate is used to replace polyglyceryl-10 stearate in an equal amount, hydrogenated lecithin is used to replace sodium stearoyl glutamate in an equal amount, and sorbitan olivate is used to replace sodium lauroyl lactylate in an equal amount.

[0148] Cetearyl glucoside was purchased from Hubei Shineng Chemical Technology Co., Ltd., CAS No.: 246159-33-1.

[0149] Polyglycerol-10 oleate was purchased from Jiangxi Huayuyuan Biotechnology Co., Ltd., CAS No.: 9007-48-1.

[0150] Hydrogenated lecithin was purchased from Xi'an Kangnuo Chemical Co., Ltd., CAS No.: 92128-87-5.

[0151] Sorbitan olivate was purchased from Wuhan Kemik Biopharmaceutical Technology Co., Ltd., CAS No.: 92128-87-5.

[0152] Experiment 1 The biomimetic liposomes simulating the intercellular lipid components of each embodiment and comparative example were placed in a transparent plastic bottle and sealed for storage. After 90 days, their appearance was observed and recorded.

[0153] The experimental data are shown in Table 1, and the appearance photos after 90 days are shown in Figure 1-8 . Appearance after 90 days of storage Example 1 Uniform and fine without particle precipitation Example 2 Uniform and fine without particle precipitation Example 3 Uniform and fine without particle precipitation Comparative Example 1 White particles precipitate Comparative Example 2 A small amount of white particles precipitated on the wall Comparative Example 3 Large white particles precipitate Comparative Example 4 Fine white particles precipitate Comparative Example 5 The paste becomes thinner and white particles precipitate

[0154] According to Table 1 and Figure 1-8From the data comparison, it can be seen that after 90 days of placement, the paste of each embodiment remains uniform and fine without particle precipitation, proving that the emulsifier in each embodiment is compounded with olive oil alcohol, polyglycerol-10 stearate, sodium stearoyl glutamate, and sodium lauroyl lactylate, so that the ceramide in the system is relatively stable in the system without crystallization, thereby maintaining the activity of ceramide well, so that the bionic liposomes simulating the intercellular lipid components of each embodiment can well play the effect of repairing the skin, and can be well applied to sebum repair, moisturizing, anti-allergic and soothing products.

[0155] In Comparative Examples 1-5, other emulsifiers are used to replace the olive oil alcohol, polyglyceryl-10 stearate, sodium stearoyl glutamate, and sodium lauroyl lactylate in the examples alone or in whole, resulting in varying degrees of crystallization and precipitation of ceramide after 90 days of storage. The precipitation of ceramide makes it difficult for ceramide to be absorbed by the skin, and the effect of using it in products such as sebum repair, moisturizing, anti-allergic and soothing is poor.

[0156] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed, but such modifications are protected by the patent law as long as they are within the scope of the claims of the present application.

Claims

1. A biomimetic liposome simulating an intercellular lipid component, characterized in that: The composition includes the following components in parts by weight: 30-38 parts of solvent; 35-43 parts of moisturizer; Oil 1.34-1.66 parts; 2.4-2.6 parts of lipids; Emulsifier 2.58-3.22 parts; 18-26 parts of skin conditioning agent; Composite ceramide 4.9-5.1 parts; 1-3 parts of phytosphingosine; 0.09-0.11 parts of Sphingomonas fermentation product extract; The emulsifier is a compound of olive oil alcohol, polyglyceryl-10 stearate, sodium stearoyl glutamate and sodium lauroyl lactylate.

2. The biomimetic liposome simulating intercellular lipid components according to claim 1, characterized in that: The mass ratio of the olive oil alcohol, polyglycerol-10 stearate, sodium stearoyl glutamate and sodium lauroyl lactylate is 1.9-2.1: 0.2-0.4: 0.4-0.6: 0.08-0.

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3. The biomimetic liposome simulating intercellular lipid components according to claim 1, characterized in that: The composite ceramide is a compound of ceramide EOP, ceramide NS / ceramide NG, ceramide NP, ceramide AS, and ceramide AP, and the mass ratio of ceramide EOP, ceramide NS / ceramide NG, ceramide NP, ceramide AS, and ceramide AP is 0.11-0.15: 0.85-0.89: 2.63-2.67: 0.33-0.37: 0.98-1.

02.

4. The biomimetic liposome simulating intercellular lipid components according to claim 1, characterized in that: The solvent is a compound of deionized water, decanol, hexyldecanol and decanediol, and the mass ratio of the deionized water, decanol, hexyldecanol and decanediol is 8-10:4-6:14-16:4-6.

5. The biomimetic liposome simulating intercellular lipid components according to claim 1, characterized in that: The moisturizing agent is a compound of panthenol, lactitol, phytol and octanediol, and the mass ratio of the panthenol, lactitol, phytol and octanediol is 13-15:4-6:9-11:9-11.

6. The biomimetic liposome simulating intercellular lipid components according to claim 1, characterized in that: The oil is a compound of meadowfoam seed oil, peony seed oil, grape seed oil and sunflower seed oil, and the mass ratio of meadowfoam seed oil, peony seed oil, grape seed oil and sunflower seed oil is 1-1.2: 0.18-0.22: 0.08-0.12: 0.08-0.

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7. The biomimetic liposome simulating intercellular lipid components according to claim 1, characterized in that: The lipid substance is cholesterol.

8. The biomimetic liposome simulating intercellular lipid components according to claim 1, characterized in that: The skin conditioning agent is a compound of hydrogenated soybean oil, oxidized corn oil, hydrogenated sunflower oil and egg yolk oil, and the mass ratio of the hydrogenated soybean oil, oxidized corn oil, hydrogenated sunflower oil and egg yolk oil is 9-11:4-6:4-6:1-3.

9. A method for preparing a biomimetic liposome simulating an intercellular lipid component according to any one of claims 1 to 8, characterized in that: The following steps are involved: Step 1), the composite ceramide, oil and lipid substances are mixed uniformly at 90-95° C. to obtain a first dispersed phase; Step 2), the solvent and the moisturizer are mixed at 80-85° C. to obtain a first mixture, the emulsifier, the skin conditioner, and the phytosphingosine are mixed at 85-90° C. to obtain a second mixture, and the first mixture and the second mixture are mixed to obtain a second dispersed phase; Step 3), mixing the first dispersed phase and the second dispersed phase, and homogenizing them to obtain a biomimetic lipid mixture; Step 4), cooling the biomimetic lipid mixture to ≤45°C, adding the extract of the fermentation product of Sphingomonas, and mixing evenly to obtain biomimetic liposomes simulating the intercellular lipid components.

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