Preparation method of trace element fertilizer containing fullerene

Abstract

The invention provides a preparation method of a micronutrient fertilizer containing fullerene, and relates to the technical field of micronutrient fertilizers, the preparation method of the micronutrient fertilizer containing fullerene comprises the following steps: spraying a nano molybdenum disulfide / fullerene suspension on the micronutrient fertilizer to obtain the micronutrient fertilizer containing fullerene after spraying, the prepared fertilizer has good stability, dispersibility and absorption efficiency, can improve the soil pH value, the soil salinity content and the soil organic matter content, can increase the yield of corn, increases the plant height, and shortens the maturation time at the same time.

Description

Technical Field

[0001] The invention relates to the technical field of trace element fertilizers, and in particular to a method for preparing a trace element fertilizer containing fullerene. Background Art

[0002] my country's saline-alkali land is widely distributed, mainly concentrated in the northwest inland, the middle and upper reaches of the Yellow River, the Huanghuai Plain, the Northeast and the coast. The saline-alkali soil contains high salt content, which has an inhibitory effect on the growth of corn. High salt content will lead to increased soil osmotic pressure, affecting the absorption of water and nutrients by the corn root system, thereby inhibiting the growth and development of corn. The saline-alkali soil is usually loose in structure and has poor water and fertilizer retention capacity, which is not conducive to the growth of the corn root system and the accumulation of nutrients. The nutrients in the saline-alkali soil are often insufficient, especially trace elements such as zinc and iron. The lack of these elements will further affect the growth and yield of corn. Saline-alkali land is often accompanied by adverse climatic conditions such as drought and high temperature. These conditions will aggravate the stress response of corn and affect its normal growth. The fertilizer used for saline-alkali crops such as corn is affected by factors such as salt and pH in the saline-alkali soil. The dissolution and release of fertilizers affect its utilization rate. Cationic trace elements (such as iron, manganese, zinc, copper, etc.) are easily adsorbed by colloids such as clay and humus in the soil to form stable complexes. This adsorption may cause the trace elements to enter the crystal lattice of soil colloids, thereby losing the ability to be absorbed and utilized by plant roots; in alkaline soils, boron may form insoluble borates and be fixed; in soils with excessive application of phosphate fertilizers, zinc, iron, manganese and other elements may combine with phosphate to form phosphate precipitation and be fixed.

[0003] Therefore, there is an urgent need in the market for fertilizers that can effectively improve saline-alkali land and increase soil fertility. Summary of the invention

[0004] The present invention provides a method for preparing a trace element fertilizer containing fullerene, so as to solve the problems raised in the above background technology.

[0005] To solve the above technical problems, the present invention discloses a method for preparing a trace element fertilizer containing fullerene, comprising spraying a nano molybdenum disulfide / fullerene suspension on the trace element fertilizer to obtain the trace element fertilizer containing fullerene after spraying.

[0006] Furthermore, 1-3 parts of the nano-molybdenum disulfide / fullerene suspension are sprayed on 8-12 parts of the trace element fertilizer by weight to obtain the trace element fertilizer containing fullerene after spraying.

[0007] Furthermore, the raw materials for preparing the nano-molybdenum disulfide / fullerene suspension include: ethanol, hexadecyltrimethylammonium bromide, polyethylene glycol, hydroxylated fullerene and nano-molybdenum disulfide.

[0008] Furthermore, the preparation method of the nano molybdenum disulfide / fullerene suspension comprises: adding 500-600 parts of ethanol, 20-30 parts of hexadecyltrimethylammonium bromide, 20-25 parts of sodium dodecylbenzenesulfonate, 10-15 parts of polyethylene glycol, 7-10 parts of hydroxylated fullerene, and 13-15 parts of nano molybdenum disulfide by weight, stirring at room temperature, ball milling, and ultrasonic reaction to obtain a surface-modified nano molybdenum disulfide fullerene suspension;

[0009] Preferably, the stirring time at room temperature is 3-8h;

[0010] Preferably, the rotation speed of the ball mill is 800-1200 rpm, and the ball-to-material ratio is 3:1;

[0011] Preferably, the ultrasonic reaction time in the ultrasonic reactor is 2-5 hours, and the ultrasonic power is 0.5 KW.

[0012] Furthermore, the preparation method of the hydroxylated fullerene comprises: by weight, putting 400-450 parts of fullerene and 400-450 parts of cerium nitrate into 1200-1500 parts of deionized water, reacting at room temperature for 4-8 hours, filtering, washing, and drying to obtain a dry product, reacting 300-500 parts of the dry product with 600-800 parts of oxidant hydrogen peroxide at 40-50° C. for 4-6 hours, filtering, washing, and drying to obtain the hydroxylated fullerene.

[0013] Furthermore, the preparation method of the trace element fertilizer comprises: taking 5-10 parts of animal manure fermented fertilizer, 1-3 parts of amino acid chelate solution, and 0.3-0.6 parts of composite bacteria, granulating, and drying to obtain the trace element fertilizer;

[0014] Preferably, the composite bacteria are Bacillus amyloliquefaciens, Bacillus licheniformis and Sphingomonas.

[0015] Furthermore, the preparation method of the animal manure fermented fertilizer comprises: mixing and fermenting, by weight, 0.3-0.5 parts of Enterococcus faecalis, 0.3-0.5 parts of Bacillus megaterium (strain concentration is 106 cfu / mg), 0.5-1 parts of maltose syrup, 1-1.5 parts of hydroxylated fullerene and 5-15 parts of water for 40-50 hours to obtain a fermented bacterial solution;

[0016] By weight, 0.5-1 parts of sugarcane molasses, 5-8 parts of corn, 15-30 parts of water and 0.5-1 parts of fermentation bacteria liquid are mixed, and fermented for 40-50 hours to obtain a primary fermentation material; 80-120 parts of pig manure and chicken manure with a water content of 60wt% and a weight ratio of 1.5:1, 0.5-1 parts of superphosphate and 2-5 parts of the primary fermentation material are mixed, and rice husks are added and stirred to obtain a secondary fermentation material; the obtained secondary fermentation material is piled and fermented to obtain animal manure fermented fertilizer.

[0017] Furthermore, the preparation method of the amino acid chelate salt solution comprises: reacting 300-400 parts of monoethanolamine with 400-450 parts of methionine and 200-250 parts of lysine by weight, centrifugally concentrating and taking the supernatant to obtain an organic amine amino acid aqueous solution, adjusting the pH to 8.5 with a calcium hydroxide aqueous solution, adding 85-100 parts of zinc sulfate heptahydrate, 20-40 parts of copper sulfate pentahydrate, 20-40 parts of manganese sulfate tetrahydrate, 20-40 parts of magnesium sulfate heptahydrate, and 30-50 parts of ferrous sulfate heptahydrate to react, and centrifugally concentrating and taking the supernatant to obtain an amino acid chelate salt solution.

[0018] Furthermore, the reaction temperature of reacting 300-400 parts of monoethanolamine with 400-450 parts of methionine and 200-250 parts of lysine is 60°C.

[0019] Furthermore, the reaction time of reacting 300-400 parts of monoethanolamine with 400-450 parts of methionine and 200-250 parts of lysine is 60-80 minutes.

[0020] Compared with the prior art, the present invention provides a method for preparing a trace element fertilizer containing fullerene, which has the following beneficial effects:

[0021] (1) Fullerene has a unique cage structure and excellent physical and chemical properties, such as good chemical stability, good mechanical stability, corrosion resistance, and antioxidant, antiviral and other biological activities. These properties enable the preparation method of trace element fertilizer containing fullerene to remain stable in the soil environment and not easily decomposed or ineffective; the antioxidant, antiviral and other biological activities can improve the stress resistance of crops and enhance the adaptability of crops to adverse conditions such as salinity and drought; hydroxylated fullerene improves the water solubility and biocompatibility of fullerene, which is conducive to better dispersion of fullerene and avoids agglomeration. Adding hydroxylated fullerene to the fermentation liquid promotes microbial growth. The high specific surface area of ​​modified fullerene may provide additional attachment sites, which is conducive to the attachment and growth of microorganisms. During the fermentation process, stable fullerene can be used as a stabilizer to protect the fermentation product from the influence of the external environment, such as oxidation and degradation; fullerene is a carbon nanomaterial with strong antioxidant ability, which can remove free radicals in plants and reduce the damage of oxidative stress to plants. In saline-alkali soil environments, fullerenes may help protect corn from oxidative damage caused by saline-alkali stress; fullerenes can absorb and transmit light energy, which may promote the photosynthesis efficiency of corn to a certain extent, and improve the light energy utilization rate and growth rate of corn;

[0022] (2) As a type of amino acid, methionine can promote the absorption and utilization of nutrients such as nitrogen, phosphorus and potassium by crops. The chelates formed by methionine and other trace elements can improve the stress resistance of crops, such as drought resistance and salt-alkali resistance. The amino acid molecules in the chelated salt solution containing methionine have high biological activity and can directly participate in the life metabolism of crops and promote the growth and development of crops.

[0023] (3) In saline-alkali land, chicken manure and pig manure containing sand, rice husks and straw can be mixed in appropriate proportions to improve the physical properties of the soil and increase its water permeability and air permeability. They are very suitable for improving soils with severe salinization and compaction. In addition, the nitrogen supply in the soil may be insufficient. Lysine is an important component of plant protein. After combining with peptides in plants, it can form a nitrogen carrier, which promotes the absorption and utilization of nitrogen in the soil by plants. Lysine can also promote the activity of glutamine synthetase in crops, thereby promoting the conversion of nitrogen to glutamine, and increasing the nitrogen content and quality of crops. Lysine can also improve the stress resistance of crops to a certain extent. Methionine and lysine work synergistically at a certain formula ratio to help crops grow better under adverse conditions such as saline-alkali land.

[0024] (4) The molybdenum in the molybdenum disulfide in the nano-molybdenum disulfide fullerene suspension is one of the trace elements required for plant growth and plays an important role in plant photosynthesis, nitrogen metabolism and other processes. The appropriate addition of molybdenum disulfide may help replenish the molybdenum element in the soil and promote the growth of corn. In addition, nano-molybdenum disulfide (MoS2) has unique physical and chemical properties. Its tiny nano size may help improve the soil structure of saline-alkali soil, increase soil porosity, and improve soil air permeability and water retention capacity. MoS2 nanoparticles may reduce the direct toxicity of salt to corn roots by absorbing and fixing salt in the soil, thereby improving the physical and chemical properties of saline-alkali soil.

[0025] (5) MoS2 nanoparticles and fullerenes can work together to enhance each other through their respective soil improvement mechanisms, further improving the aeration, water retention and nutrient supply capacity of saline-alkali soils. Through their surface effects and adsorption capacity, they can improve the utilization rate of nutrients such as nitrogen, phosphorus and potassium in the soil. The antioxidant effect of fullerenes may protect the corn root system from damage caused by saline-alkali stress, allowing it to maintain better absorption capacity. At the same time, the molybdenum element and other nutrients provided by MoS2 nanoparticles may exist in a more easily absorbed form. The two work together to promote the absorption and utilization of nutrients by corn, enhance corn's resistance to saline-alkali stress, and improve corn's survival ability and growth rate in saline-alkali soil environments. They can improve soil pH, soil salt content and soil organic matter content, while increasing corn yield, increasing plant height and shortening maturity time.

[0026] (6) In order to ensure that the fermented bacterial liquid meets the use standards and to ensure the quality of animal manure fermented fertilizer, monitoring information of the fermentation process is collected when the fermentation liquid is prepared. Then, after the fermentation is completed, the monitoring liquid is sampled and decomposed to determine whether the fermented liquid is qualified. Compensatory fermentation is performed on the unqualified liquid to ensure that the fermented liquid meets the use standards. In this way, fermentation monitoring can be realized and abnormal factors in the fermentation process can be decomposed to avoid affecting subsequent work. DETAILED DESCRIPTION

[0027] The preferred embodiments of the present invention are described below. It should be understood that the preferred embodiments described herein are only used to illustrate and explain the present invention, and are not used to limit the present invention.

[0028] In addition, in the present invention, the descriptions of "first", "second", etc. are only used for descriptive purposes, and do not specifically refer to the order or sequence, nor are they used to limit the present invention. They are only used to distinguish components or operations described with the same technical terms, and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In addition, the technical solutions and technical features between the various embodiments can be combined with each other, but they must be based on the ability of ordinary technicians in the field to implement them. When the combination of technical solutions is contradictory or cannot be implemented, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection required by the present invention.

[0029] Unless otherwise specified, the examples and comparative examples are parallel tests with the same components, component contents, preparation steps and preparation parameters. The experimental methods in the following examples are conventional methods unless otherwise specified. The test materials used in the following examples are analytical reagents (AR) unless otherwise specified. Parts are parts by weight unless otherwise specified, and the materials are purchased from commercial channels.

[0030] Fullerene was purchased from Sigma Aldrich, CAS No.: 99685-96-8, with a molecular weight of 720.64 and a purity of 99.5%; nano-molybdenum disulfide was purchased from Mack Lin, CAS No.: 1317-33-5, with a purity of ≥99.5%, 100 nm; maltose syrup was purchased from Jinan Tianhua Biotechnology Co., Ltd., CAS No.: 20883-2007, with a content of 75%; sugarcane molasses was purchased from Guangxi Huashun International Economic and Trade Co., Ltd.; methionine was purchased from Sigma Aldrich, CAS No.: 63-68-3; lysine was purchased from Sigma Aldrich, CAS: 657-27-2; and rice husk was purchased from Shandong Hongyuan Biotechnology Co., Ltd.

[0031] Bacillus amyloliquefaciens, Bacillus licheniformis, Sphingomonas, Enterococcus faecalis (CGMCC No. 1.15424), and Bacillus megaterium were purchased from China General Microbiological Culture Collection Center (CGMCC) and China Center for Type Culture Collection (CCTCC).

[0032] Example 1

[0033] 1. Preparation of hydroxylated fullerene:

[0034] 400 parts of fullerene and 400 parts of cerium nitrate were placed in 1200 parts of deionized water, reacted at room temperature for 4 hours, filtered, washed and dried to obtain a dry product, 300 parts of the dry product and 600 parts of oxidant hydrogen peroxide were reacted at 40°C for 4 hours, filtered, washed and dried to obtain hydroxylated fullerene.

[0035] 2. Preparation of animal manure fermentation fertilizer:

[0036] 0.3 parts of Enterococcus faecalis, 0.3 parts of Bacillus megaterium (strain concentration is 106 cfu / mg), 0.5 parts of maltose syrup, 1 part of hydroxylated fullerene and 5 parts of water are mixed and fermented for 40 hours to obtain a fermentation solution;

[0037] 0.5 parts of sugarcane molasses, 5 parts of corn, 15 parts of water and 0.5 parts of fermentation liquid are mixed, and fermented for 40 hours to obtain a primary fermentation material; 80 parts of pig manure and chicken manure with a water content of 60wt% and a weight ratio of 1.5:1, 0.5 parts of superphosphate are mixed with 2 parts of the primary fermentation material, and 80-mesh rice husks are added and stirred to obtain a secondary fermentation material; the prepared secondary fermentation material is piled to a height of 1m, a length of 2m and a width of 1m, covered with a film, fermented at 20°C for 72h, heated to 60°C, and fermented for 36h to obtain animal manure fermented fertilizer.

[0038] 3. Preparation of amino acid chelate solution:

[0039] 300 parts of monoethanolamine are reacted with 400 parts of methionine and 200 parts of lysine, the temperature is controlled at 60°C for chelation reaction for 60 minutes, the supernatant is centrifuged and concentrated to obtain an organic amine amino acid aqueous solution, the pH is adjusted to 8.5 with a 0.5 mol / l calcium hydroxide aqueous solution, 85 parts of zinc sulfate heptahydrate, 20 parts of copper sulfate pentahydrate, 20 parts of manganese sulfate tetrahydrate, 20 parts of magnesium sulfate heptahydrate and 30 parts of ferrous sulfate heptahydrate are added, the mixture is reacted at 20°C for 80 minutes, and the supernatant is centrifuged and concentrated to obtain an amino acid chelate salt solution.

[0040] 4. Preparation of trace element fertilizer:

[0041] Take 5 parts of animal manure fermented fertilizer, 1 part of amino acid chelate solution, and 0.3 parts of compound bacteria, mix them, granulate, and dry them to obtain trace element fertilizer. The compound bacteria are 0.1 parts of Bacillus amyloliquefaciens, 0.1 parts of Bacillus licheniformis, and 0.1 parts of Sphingomonas. The strain concentration is 10 4 Calculated in cfu / mg.

[0042] 5. Preparation of Nano MoS2 / Fullerene Suspension

[0043] 500 parts of ethanol, 20 parts of hexadecyltrimethylammonium bromide, 20 parts of sodium dodecylbenzenesulfonate, 10 parts of polyethylene glycol, 7 parts of hydroxylated fullerene and 13 parts of nano molybdenum disulfide were added to a stirring kettle by weight. After mechanical stirring for 3 hours at room temperature, the mixture was added to a ball mill for ball milling (rotation speed 800 rpm, ball-to-material ratio 3:1), and finally placed in an ultrasonic reactor for treatment for 2 hours (ultrasonic power 0.5 KW) to obtain a surface-modified nano molybdenum disulfide fullerene suspension.

[0044] 6. Preparation of trace element fertilizer containing fullerene:

[0045] One part of nano molybdenum disulfide / fullerene suspension is sprayed on 9 parts of trace element fertilizer to obtain the trace element fertilizer containing fullerene after spraying.

[0046] Example 2

[0047] 1. Preparation of hydroxylated fullerene:

[0048] 420 parts of fullerene and 410 parts of cerium nitrate were placed in 1300 parts of deionized water, reacted at room temperature for 5 hours, filtered, washed and dried to obtain a dry product, 400 parts of the dry product and 700 parts of oxidant hydrogen peroxide were reacted at 50°C for 5 hours, filtered, washed and dried to obtain hydroxylated fullerene.

[0049] 2. Preparation of animal manure fermentation fertilizer:

[0050] 0.4 parts of Enterococcus faecalis, 0.5 parts of Bacillus megaterium (strain concentration is 106 cfu / mg), 0.8 parts of maltose syrup, 1.3 parts of hydroxylated fullerene and 10 parts of water are mixed and fermented for 45 hours to obtain a fermentation solution;

[0051] 0.8 parts of sugarcane molasses, 6 parts of corn, 20 parts of water and 0.8 parts of fermentation liquid are mixed, and fermented for 45 hours to obtain a primary fermentation material; 100 parts of pig manure and chicken manure with a water content of 60wt% and a weight ratio of 1.5:1, 0.8 parts of superphosphate are mixed with 4 parts of the primary fermentation material, and 60-mesh rice husks are added and stirred to obtain a secondary fermentation material; the obtained secondary fermentation material is piled to a height of 1m, a length of 2m and a width of 1m, covered with a film, fermented at 20°C for 72h, heated to 60°C, and fermented for 36h to obtain animal manure fermented fertilizer.

[0052] 3. Preparation of amino acid chelate solution:

[0053] 350 parts of monoethanolamine are reacted with 450 parts of methionine and 230 parts of lysine, the temperature is controlled at 60°C for chelation reaction for 80 minutes, the supernatant is centrifuged and concentrated to obtain an organic amine amino acid aqueous solution, the pH is adjusted to 8.5 with a 0.5 mol / l calcium hydroxide aqueous solution, 90 parts of zinc sulfate heptahydrate, 33 parts of copper sulfate pentahydrate, 38 parts of manganese sulfate tetrahydrate, 32 parts of magnesium sulfate heptahydrate and 40 parts of ferrous sulfate heptahydrate are added, the reaction is carried out at 30°C for 60 minutes, and the supernatant is centrifuged and concentrated to obtain an amino acid chelate salt solution.

[0054] 4. Preparation of trace element fertilizer:

[0055] Take 8 parts of animal manure fermented fertilizer, 2 parts of amino acid chelate solution, and 0.5 parts of compound bacteria, mix them, granulate, and dry them to obtain trace element fertilizer. The compound bacteria are 0.2 parts of Bacillus amyloliquefaciens, 0.2 parts of Bacillus licheniformis, and 0.1 parts of Sphingomonas. The strain concentration is 10 4 Calculated in cfu / mg.

[0056] 5. Preparation of Nano MoS2 / Fullerene Suspension

[0057] 550 parts of ethanol, 25 parts of hexadecyltrimethylammonium bromide, 23 parts of sodium dodecylbenzenesulfonate, 12 parts of polyethylene glycol, 8 parts of hydroxylated fullerene and 14 parts of nano molybdenum disulfide were added to a stirring kettle by weight. After mechanical stirring for 6 hours at room temperature, the mixture was added to a ball mill for ball milling (rotation speed 800 rpm, ball-to-material ratio 3:1), and finally placed in an ultrasonic reactor for treatment for 4 hours (ultrasonic power 0.5 KW) to obtain a surface-modified nano molybdenum disulfide fullerene suspension.

[0058] 6. Preparation of trace element fertilizer containing fullerene:

[0059] Two parts of nano-molybdenum disulfide / fullerene suspension are sprayed on eight parts of trace element fertilizer to obtain the trace element fertilizer containing fullerene after spraying.

[0060] Example 3

[0061] 1. Preparation of hydroxylated fullerene:

[0062] 450 parts of fullerene and 450 parts of cerium nitrate were placed in 1500 parts of deionized water, reacted at room temperature for 8 hours, filtered, washed and dried to obtain a dry product, 500 parts of the dry product and 800 parts of oxidant hydrogen peroxide were reacted at 50°C for 6 hours, filtered, washed and dried to obtain hydroxylated fullerene.

[0063] 2. Preparation of animal manure fermentation fertilizer:

[0064] 0.5 parts of Enterococcus faecalis and 0.5 parts of Bacillus megaterium (strain concentration was 10 6cfu / mg), 1 part of maltose syrup, 1.5 parts of hydroxylated fullerene and 15 parts of water were mixed and fermented for 50 hours to obtain a fermentation solution;

[0065] Mix 1 part of sugarcane molasses, 8 parts of corn, 30 parts of water and 1 part of fermentation bacteria liquid, and ferment for 50 hours to obtain a primary fermentation material; mix 120 parts of pig manure and chicken manure with a water content of 60wt% and a weight ratio of 1.5:1, 1 part of superphosphate and 5 parts of the primary fermentation material, add 60-mesh rice husks, stir to obtain a secondary fermentation material; pile the obtained secondary fermentation material to a height of 1m, a length of 2m and a width of 1m, cover with a film, ferment at 20°C for 72h, heat to 60°C, and continue to ferment for 36h to obtain animal manure fermented fertilizer.

[0066] 3. Preparation of amino acid chelate solution:

[0067] 400 parts of monoethanolamine are reacted with 500 parts of methionine and 250 parts of lysine, the temperature is controlled at 60°C for chelation reaction for 60 minutes, the supernatant is centrifuged and concentrated to obtain an organic amine amino acid aqueous solution, the pH is adjusted to 8.5 with a 0.5 mol / l calcium hydroxide aqueous solution, 100 parts of zinc sulfate heptahydrate, 40 parts of copper sulfate pentahydrate, 40 parts of manganese sulfate tetrahydrate, 40 parts of magnesium sulfate heptahydrate and 50 parts of ferrous sulfate heptahydrate are added, the reaction is carried out at 30°C for 80 minutes, and the supernatant is centrifuged and concentrated to obtain an amino acid chelate salt solution.

[0068] 4. Preparation of trace element fertilizer:

[0069] Take 10 parts of animal manure fermented fertilizer, 3 parts of amino acid chelate solution, and 0.6 parts of compound bacteria, mix them, granulate, and dry them to obtain trace element fertilizer. The compound bacteria are 0.3 parts of Bacillus amyloliquefaciens, 0.2 parts of Bacillus licheniformis, and 0.1 parts of Sphingomonas. The strain concentration is 10 4 Calculated in cfu / mg.

[0070] 5. Preparation of Nano MoS2 / Fullerene Suspension

[0071] 600 parts of ethanol, 30 parts of hexadecyltrimethylammonium bromide, 25 parts of sodium dodecylbenzenesulfonate, 15 parts of polyethylene glycol, 10 parts of hydroxylated fullerene and 15 parts of nano molybdenum disulfide were added to a stirring tank by weight. After mechanical stirring for 8 hours at room temperature, the mixture was added to a ball mill for ball milling (rotation speed 1200 rpm, ball-to-material ratio 3:1), and finally placed in an ultrasonic reactor for treatment for 5 hours (ultrasonic power 0.5 KW) to obtain a surface-modified nano molybdenum disulfide fullerene suspension.

[0072] 6. Preparation of trace element fertilizer containing fullerene:

[0073] Three parts of nano-molybdenum disulfide / fullerene suspension are sprayed on 12 parts of trace element fertilizer to obtain the trace element fertilizer containing fullerene after spraying.

[0074] Example 4

[0075] On the basis of Example 2, the invention further includes:

[0076] In the process of mixing and fermenting 0.3 parts of Enterococcus faecalis, 0.3 parts of Bacillus megaterium (strain concentration is 106 cfu / mg), 0.5 parts of maltose syrup, 1 part of hydroxylated fullerene and 5 parts of water for 40 hours to obtain a fermentation solution:

[0077] Collecting a plurality of monitoring information during the fermentation process, determining the fermentation progress of the fermentation process based on the monitoring information, and sampling the monitoring liquid to obtain the sampled liquid when the fermentation progress is 100%;

[0078] Decomposing the sampled liquid in multiple dimensions to obtain the qualification level of the monitored liquid, and when the qualification level does not meet the qualification standard, establishing a dynamic fermentation process of the monitored liquid according to the monitoring information;

[0079] Simulating the dynamic fermentation process to determine the corresponding product concentration and by-product concentration at different times during the fermentation process, and generating product-by-product comparison information of the fermentation process;

[0080] Determine several product unqualified moments and by-product unqualified moments of the fermentation process according to the product-by-product comparison information;

[0081] Respectively obtaining first monitoring information corresponding to the moment when each product fails to meet the standard and second monitoring information corresponding to the moment when each by-product fails to meet the standard;

[0082] Performing a first abnormal screening on the first monitoring information to determine a plurality of first abnormal factors of the current fermentation process, and performing a second abnormal screening on the second monitoring information to determine a plurality of second abnormal factors of the current fermentation process;

[0083] Using the first abnormal factor and the second abnormal factor in combination with the monitoring time corresponding to each abnormal factor, an abnormal factor queue of the fermentation process is established;

[0084] Using a clustering algorithm to divide the abnormal factor queue into a number of clustered abnormal factors, and obtaining an abnormal compensation measure corresponding to each clustered abnormal factor;

[0085] Determine the abnormality degree of each abnormal factor based on the abnormal factor queue, and use the abnormality degree to adjust the parameters of the corresponding abnormal supplementary measures to generate a perfect compensation method;

[0086] A perfect compensation environment is constructed according to the perfect compensation method, and the monitoring liquid is placed in the perfect compensation environment for compensatory fermentation to obtain a fermentation bacterial liquid for this fermentation.

[0087] In this example, the multi-dimensional decomposition includes: pH value, gas composition, liquid composition;

[0088] In this example, the qualified standard means the fermentation liquid meets the use requirements;

[0089] In this example, the first monitoring information represents information presented when an abnormality occurs in a product, and the second monitoring information represents information presented when an abnormality occurs in a by-product;

[0090] In this example, clustering abnormal factors means the result of clustering abnormal factors with the same abnormal characteristics together;

[0091] In this example, the abnormality compensation measure refers to a measure for eliminating the abnormal factor.

[0092] The working principle and beneficial effects of the above technical solution are as follows: In order to ensure that the fermented bacterial liquid meets the use standards and ensure the quality of animal manure fermented fertilizer, monitoring information of the fermentation process is collected when making the fermentation liquid, and then the monitoring liquid is sampled after the fermentation is completed. The sampled liquid is decomposed to determine whether the fermented liquid is qualified, and compensatory fermentation is performed on the unqualified liquid to ensure that the fermented liquid meets the use standards. In this way, fermentation monitoring can be realized, and abnormal factors in the fermentation process can be decomposed to avoid affecting subsequent work.

[0093] Example 5

[0094] On the basis of Example 4, it also includes:

[0095] When the qualification degree of the monitoring liquid meets the qualification standard, the monitoring liquid is regarded as the fermentation bacterial liquid.

[0096] The working principle and beneficial effects of the above technical solution: In order to avoid the phenomenon of over-compensation, when the monitored liquid meets the qualified standards, it is regarded as fermentation liquid and put into the next step.

[0097] Comparative Example 1

[0098] The difference from Example 1 is that when preparing the suspension, an equal weight portion of nano-molybdenum disulfide is missing, and a fullerene suspension is prepared, and the rest is the same.

[0099] Comparative Example 2

[0100] The difference from Example 1 is that when preparing the suspension, an equal weight portion of hydroxylated fullerene is lacking, and a nano-molybdenum disulfide suspension is prepared, and the rest is the same.

[0101] Comparative Example 3

[0102] The difference from Example 1 is that the step of modifying fullerene is omitted (the preparation of hydroxylated fullerene is omitted), and fullerene in equal parts by weight is directly used, and the rest are the same.

[0103] Comparative Example 4

[0104] The difference from Example 1 is that an equal weight portion of amino acid chelate salt solution is missing when preparing the trace element fertilizer, and the other parts are the same.

[0105] Comparative Example 5

[0106] The difference from Example 1 is that an equal weight portion of hydroxylated fullerene is missing when preparing the animal manure fermented fertilizer, and the rest is the same.

[0107] Comparative Example 6

[0108] The difference from Example 1 is that the spraying of the nano-molybdenum disulfide / fullerene suspension in equal parts by weight is missing, and the other parts are the same.

[0109] Performance Testing

[0110] The test site was conducted in saline-alkali land in Hainan. The soil pH value (average value) of the saline-alkali land test field was 8.32 when no fertilizer was applied, the soil salt content (g / kg) was 4.62, and the soil organic matter content (%) was 1.21. The corn variety Zhengdan 958 was planted, and the soil tillage layer was 35 cm. The products prepared in Example 1 and the comparative example were used for fertilization to ensure that the fertilizer content of the experimental products was the same. Fertilizer was applied at an amount of 60 kg per mu, the distance between the fertilizer row and the seed row was 10 cm, and the depth of the fertilizer row was 15 cm. The performance measurement results are shown in Table 1 below. The distance from the ground to the top of the corn plant measured with a tape measure is its plant height.

[0111] Table 1

[0112]

[0113] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. If these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. A method for preparing a trace element fertilizer containing fullerenes, comprising spraying a nano molybdenum disulfide / fullerene suspension on the trace element fertilizer to obtain the trace element fertilizer containing fullerenes after spraying.

2. The method for preparing the trace element fertilizer containing fullerene according to claim 1, characterized in that: According to the weight percentage, 1-3 parts of nano molybdenum disulfide / fullerene suspension are sprayed on 8-12 parts of trace element fertilizer to obtain the trace element fertilizer containing fullerene after spraying.

3. The method for preparing the trace element fertilizer containing fullerene according to claim 1 or 2, characterized in that: The raw materials for preparing the nano molybdenum disulfide / fullerene suspension include: ethanol, hexadecyltrimethylammonium bromide, polyethylene glycol, hydroxylated fullerene and nano molybdenum disulfide.

4. The method for preparing the trace element fertilizer containing fullerene according to claim 3, characterized in that: The preparation method of the nano molybdenum disulfide / fullerene suspension comprises: adding 500-600 parts of ethanol, 20-30 parts of hexadecyltrimethylammonium bromide, 20-25 parts of sodium dodecylbenzenesulfonate, 10-15 parts of polyethylene glycol, 7-10 parts of hydroxylated fullerene, and 13-15 parts of nano molybdenum disulfide by weight, stirring at room temperature, ball milling, and ultrasonic reaction to obtain a surface-modified nano molybdenum disulfide fullerene suspension; Preferably, the stirring time at room temperature is 3-8h; Preferably, the rotation speed of the ball mill is 800-1200 rpm, and the ball-to-material ratio is 3:1; Preferably, the ultrasonic reaction time in the ultrasonic reactor is 2-5 hours, and the ultrasonic power is 0.5 KW.

5. The method for preparing the trace element fertilizer containing fullerene according to claim 3 or 4, characterized in that: The preparation method of the hydroxylated fullerene comprises: by weight, putting 400-450 parts of fullerene and 400-450 parts of cerium nitrate into 1200-1500 parts of deionized water, reacting at room temperature for 4-8 hours, filtering, washing and drying to obtain a dried product, reacting 300-500 parts of the dried product and 600-800 parts of oxidant hydrogen peroxide at 40-50° C. for 4-6 hours, filtering, washing and drying to obtain the hydroxylated fullerene.

6. The method for preparing the trace element fertilizer containing fullerene according to claim 2, characterized in that: The preparation method of the trace element fertilizer comprises: taking 5-10 parts by weight of animal manure fermented fertilizer, 1-3 parts of amino acid chelate solution, and 0.3-0.6 parts of composite bacteria, mixing, granulating, and drying to obtain the trace element fertilizer; Preferably, the composite bacteria are Bacillus amyloliquefaciens, Bacillus licheniformis and Sphingomonas.

7. The method for preparing the trace element fertilizer containing fullerene according to claim 6, characterized in that: The method for preparing the animal manure fermented fertilizer comprises: mixing 0.3-0.5 parts by weight of Enterococcus faecalis, 0.3-0.5 parts by weight of Bacillus megaterium, 0.5-1 parts by weight of maltose syrup, 1-1.5 parts by weight of hydroxylated fullerene and 5-15 parts by weight of water for 40-50 hours to obtain a fermented bacterial solution; By weight, 0.5-1 parts of sugarcane molasses, 5-8 parts of corn, 15-30 parts of water and 0.5-1 parts of fermentation bacteria liquid are mixed, and fermented for 40-50 hours to obtain a primary fermentation material; 80-120 parts of pig manure and chicken manure with a water content of 60wt% and a weight ratio of 1.5:1, 0.5-1 parts of superphosphate and 2-5 parts of the primary fermentation material are mixed, and rice husks are added and stirred to obtain a secondary fermentation material; the obtained secondary fermentation material is piled and fermented to obtain animal manure fermented fertilizer.

8. The method for preparing the trace element fertilizer containing fullerene according to claim 6, characterized in that: The preparation method of the amino acid chelate salt solution comprises: reacting 300-400 parts of monoethanolamine with 400-450 parts of methionine and 200-250 parts of lysine by weight, centrifugally concentrating and taking the supernatant to obtain an organic amine amino acid aqueous solution, adjusting the pH to 8.5 with a calcium hydroxide aqueous solution, adding 85-100 parts of zinc sulfate heptahydrate, 20-40 parts of copper sulfate pentahydrate, 20-40 parts of manganese sulfate tetrahydrate, 20-40 parts of magnesium sulfate heptahydrate and 30-50 parts of ferrous sulfate heptahydrate for reaction, and centrifugally concentrating and taking the supernatant to obtain the amino acid chelate salt solution.

9. The method for preparing the trace element fertilizer containing fullerene according to claim 8, characterized in that: The reaction temperature for reacting 300-400 parts of monoethanolamine with 400-450 parts of methionine and 200-250 parts of lysine is 60° C., based on weight.

10. The method for preparing the trace element fertilizer containing fullerene according to claim 8, characterized in that: The reaction time for reacting 300-400 parts of monoethanolamine with 400-450 parts of methionine and 200-250 parts of lysine is 60-80 minutes based on weight.

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