A synbiotic feed additive and its preparation method and application

Abstract

The present application relates to the technical field of feed additives, and specifically discloses a synbiotic feed additive and its preparation method and application. The synbiotic feed additive provided in the present application includes the following components in parts by weight: 50-100 parts of prebiotics, 10-20 parts of probiotics and 5-15 parts of plant extracts; the prebiotics include Poria cocos polysaccharide and pullulan polysaccharide in a weight ratio of 1: (0.2-0.5); the probiotics include Lactobacillus helveticus, Acetobacter orlanii and Streptomyces virginiana; wherein the plant extract is selected from yam extract, soybean extract, astragalus polysaccharide and allicin; the present application also provides a method for preparing the above-mentioned synbiotic feed additive. The synbiotic feed additive provided in the present application can regulate the balance of intestinal microorganisms in animals, improve the digestion and absorption function of animals, and thereby improve the growth performance and feed conversion rate of animals.

Description

Technical Field

[0001] The present application relates to the technical field of feed additives, and in particular to a synbiotic feed additive and a preparation method and application thereof. Background Art

[0002] With the rapid development of animal husbandry, large-scale farming has become the mainstream trend, which not only improves farming efficiency but also brings significant economic benefits. However, in this context, problems such as low immune system, slow growth and frequent diseases caused by imbalance of animal intestinal microecology have become increasingly prominent, seriously affecting farming benefits and animal health.

[0003] In order to deal with the above problems, the existing solutions are mainly to adjust the balance of animal intestinal microorganisms through feed additives, improve the digestion and absorption function of animals, and thus improve the overall health level of animals. At present, commonly used feed additives include probiotics, prebiotics and antibiotics; however, the use effect of existing probiotics and prebiotics is very limited, especially in complex breeding environments, it is difficult to comprehensively solve the problem of intestinal microecological imbalance; in addition, probiotics and prebiotics are easily destroyed by gastric acid when passing through the stomach, resulting in low bioavailability and difficulty in fully exerting their effects. Although the use of antibiotics can control diseases in the short term, long-term use will lead to drug residues and drug resistance, posing a hidden danger to food safety.

[0004] Therefore, it is of great significance to the livestock and poultry farming industry to develop a feed additive that can regulate the balance of animal intestinal microorganisms, improve the animal's digestion and absorption function, and thus improve the animal's growth performance and feed conversion rate. Summary of the invention

[0005] In order to overcome the deficiencies in the prior art and improve the growth performance and feed conversion rate of animals, the present application provides a synbiotic feed additive and a preparation method and application thereof.

[0006] In the first aspect, the present application provides a synbiotic feed additive, which adopts the following technical solution:

[0007] A synbiotic feed additive, comprising the following components in parts by weight: 50-100 parts of prebiotics, 10-20 parts of probiotics and 5-15 parts of plant extracts;

[0008] The prebiotics include tuckahoe polysaccharide and pullulan in a weight ratio of 1:(0.2-0.5); the probiotics include Lactobacillus helveticus, Acetobacter orlanii and Streptomyces virginianus.

[0009] The present application provides a synbiotic feed additive, which can significantly improve the balance of animal intestinal microecology, enhance the immune and disease resistance function and digestion and absorption function of animals, and promote the healthy and rapid growth of animals. Specifically, on the one hand, Poria cocos polysaccharide can enhance the immune function of animals and improve the resistance of animals to diseases; on the other hand, it can improve the intestinal barrier function of animals, promote intestinal health, reduce diarrhea rate, thereby improving the production performance and feed conversion rate of animals; Pullulan, as a water-soluble mucous polysaccharide, has a good thickening effect on the one hand, can improve the taste and texture of feed, and make it easier to be digested and absorbed by animals; on the other hand, Pullulan can be used by certain intestinal microorganisms to promote the proliferation of beneficial bacteria, maintain the balance of intestinal flora, thereby improving the intestinal health of animals, improving their digestion and absorption capacity of feed, and improving feed conversion rate. The present application, by compounding Poria cocos polysaccharide and pullulan polysaccharide in a specific ratio, can form a microenvironment in the intestine that is conducive to the proliferation of beneficial flora, thereby inhibiting the growth of harmful bacteria, maintaining intestinal health, and then promoting the digestion and absorption of feed by animals, improving their growth performance and feed conversion rate. The synergistic effect of the three probiotics, Lactobacillus helveticus, Acetobacter orlanii, and Streptomyces virginiana, can further enhance the number and activity of beneficial bacteria in the intestine, promote the absorption and metabolism of nutrients by animals, and improve the overall health of animals. In summary, the synbiotic feed additive provided by the present application can well regulate the balance of animal intestinal microorganisms, improve the growth performance and feed conversion rate of animals, and improve the breeding efficiency of livestock and poultry farming.

[0010] In some embodiments, the weight ratio of the pachymaran to the pullulan may be 1:(0.2-0.3), 1:(0.2-0.4), 1:(0.2-0.5), (0.3-0.4), 1:(0.3-0.5) or 1:(0.4-0.5).

[0011] In a specific embodiment, the weight ratio of the pachymaran to the pullulan can also be 1:0.2, 1:0.3, 1:0.4 or 1:0.5.

[0012] Optionally, the weight ratio of Lactobacillus helveticus, Acetobacter orlanii and Streptomyces virginiana is 1:(0.35-0.55):(0.60-0.75).

[0013] Optionally, the weight ratio of Lactobacillus helveticus, Acetobacter orlanii and Streptomyces virginiana is 1:(0.4-0.5):(0.65-0.7).

[0014] In some embodiments, the weight ratio of Lactobacillus helveticus, Acetobacter orlanii, and Streptomyces virginiae can be 1:(0.35-0.4):0.65, 1:(0.35-0.45):0.65, 1:(0.35-0.5):0.65, 1:(0.35-0.55):0.65, 1:(0.4-0.45):0.65, 1:(0.4-0.5):0.65, 1:(0.4-0.55):0.6 5. 1: (0.45-0.5): 0.65, 1: (0.45-0.55): 0.65 or 1: (0.5-0.55): 0.65, 1: 0.45: (0.6-0.65), 1: 0.45: (0.6-0.7), 1: 0.45: (0.6-0.75), 1: 0.45: (0.65-0.7), 1: 0.45: (0.65-0.75) or 1: 0.45: (0.7-0.75).

[0015] In a specific embodiment, the weight ratio of Lactobacillus helveticus, Acetobacter orlanii and Streptomyces virginia may also be 1:0.35:0.65, 1:0.4:0.65, 1:0.45:0.65, 1:0.5:0.65, 1:0.55:0.65, 1:0.45:0.6, 1:0.45:0.7 or 1:0.45:0.75.

[0016] Optionally, the plant extract is selected from yam extract, soybean extract, astragalus polysaccharide and allicin.

[0017] In some embodiments, the plant extract may be a mixture of soybean extract and astragalus polysaccharide, a mixture of soybean extract and allicin, or a mixture of yam extract and astragalus polysaccharide.

[0018] Optionally, the plant extract is a mixture of soybean extract and astragalus polysaccharide.

[0019] In this application, the addition of plant extracts can not only provide rich vitamins and trace elements, but also have certain antioxidant and antibacterial effects, further enhance the immunity and anti-stress ability of animals, and improve the growth performance of animals. Through experimental research, this application found that the use of a mixture of soybean extract and astragalus polysaccharide as a plant extract can produce a synbiotic feed additive with better use effect and better growth promotion effect on animals.

[0020] Optionally, the weight ratio of the soybean extract to astragalus polysaccharide is 1:(2-4).

[0021] In some embodiments, the weight ratio of the soybean extract to astragalus polysaccharide may be 1:(2-3) or 1:(3-4).

[0022] In a specific embodiment, the weight ratio of the soybean extract to astragalus polysaccharide can be 1:2, 1:3 or 1:4.

[0023] Optionally, the synbiotic feed additive also includes 30-40 parts of soluble starch.

[0024] In a second aspect, the present application provides a method for preparing a synbiotic feed additive, comprising the following steps: first, prebiotics, probiotics and plant extracts are mixed evenly to obtain a premix; then water is added to adjust the water content of the premix to 12±2%, and granulated with a granulator, and dried to obtain a synbiotic feed additive with a particle size of 3-5 mm.

[0025] In a third aspect, the present application provides an animal feed comprising a synbiotic feed additive.

[0026] Optionally, the synbiotic feed additive is added to animal feed in an amount of 0.8-1.5 g / kg.

[0027] In summary, this application has the following beneficial effects:

[0028] 1. This application uses Poria cocos polysaccharide and pullulan as prebiotics, Lactobacillus helveticus, Acetobacter orlanii and Streptomyces virginiana as probiotics to prepare a synbiotic feed additive. The synbiotic feed additive can improve the balance of animal intestinal microecology, enhance the animal's immune and disease resistance and digestion and absorption functions, improve animal growth performance and feed conversion rate, thereby increasing the breeding benefits of farms, and has good economic value and application prospects.

[0029] 2. The present application further controls the weight ratio of Lactobacillus helveticus, Acetobacter orlanii and Streptomyces virginiana in the probiotics within the range of 1: (0.4-0.5): (0.65-0.7), and the obtained synbiotic feed additive has a better use effect and can make the feed conversion rate of broilers reach below 1.5.

[0030] 3. The present application further adopts soybean extract and astragalus polysaccharide in a weight ratio of 1: (2-4) as plant extracts to prepare a synbiotic feed additive, which has a better effect and can make the feed conversion rate of broilers reach below 1.45. DETAILED DESCRIPTION

[0031] The present application provides a synbiotic feed additive, comprising the following components in parts by weight: 50-100 parts of prebiotics, 10-20 parts of probiotics, 5-15 parts of plant extracts and 30-40 parts of starch-soluble starch; the prebiotics include tuckahoe polysaccharide and pullulan polysaccharide in a weight ratio of 1: (0.2-0.5); the probiotics include Lactobacillus helveticus, Acetobacter orlanis and Streptomyces virginianus. Further, the weight ratio of Lactobacillus helveticus, Acetobacter orlanis and Streptomyces virginianus is 1: (0.35-0.55): (0.60-0.75). Further, the weight ratio of Lactobacillus helveticus, Acetobacter orlanis and Streptomyces virginianus is 1: (0.4-0.5): (0.65-0.7);.

[0032] Wherein, the plant extract is selected from yam extract, soybean extract, astragalus polysaccharide and allicin; further, the plant extract is a mixture of soybean extract and astragalus polysaccharide; further, the weight ratio of the soybean extract to astragalus polysaccharide is 1: (2-4).

[0033] The preparation method of the above-mentioned synbiotic feed additive comprises the following steps: firstly, prebiotics, probiotics, plant extracts and corn starch are mixed evenly to obtain a premix; then, water is added to adjust the water content of the premix to 12±2%, and granulation is performed using a granulator, and drying is performed to obtain a synbiotic feed additive with a particle size of 3-5 mm.

[0034] In the embodiments of the present application, Poria polysaccharide was purchased from Shaanxi Snot Biotechnology Co., Ltd.; pullulan polysaccharide was purchased from Zhengzhou Mingxin Food Ingredients Co., Ltd.; Lactobacillus helveticus was purchased from Jiangsu Caiwei Biotechnology Co., Ltd. as freeze-dried powder; Acetobacter orlanis, also known as Acetobacter orlanis subspecies, freeze-dried powder, was purchased from Shanghai Guyan Industrial Co., Ltd.; Streptomyces virginiana was purchased from Ruichu Biotechnology (Jiangsu) Co., Ltd. as freeze-dried powder; yam extract was purchased from Shanyang Lianfeng Biotechnology Co., Ltd.; soybean extract was purchased from Fufeng Snot Biotechnology Co., Ltd.; Astragalus polysaccharide was purchased from Shaanxi Baichuan Biotechnology Co., Ltd.; Allicin was purchased from Jinan Xinhuasheng Biotechnology Co., Ltd.; the remaining raw materials, reagents, solvents, etc. used in this application can be obtained commercially.

[0035] The present application is further described in detail below in conjunction with embodiments and performance testing experiments.

[0036] Examples 1-4

[0037] Examples 1-4 each provide a synbiotic feed additive.

[0038] The difference between the above embodiments is that the components and proportions of prebiotics are specifically shown in Table 1 below.

[0039] The preparation method of the synbiotic feed additive provided in Examples 1-4 comprises the following steps: first, 80 g of prebiotics, 15 g of probiotics (Lactobacillus helveticus, Acetobacter orlanii and Streptomyces virginianus in a weight ratio of 1:0.35:0.65), 10 g of soybean extract and 30 g of corn starch are uniformly mixed to obtain a premix; then, water is added to adjust the water content of the premix to 12±2%, and granulation is performed using a granulator, and drying is performed to obtain a synbiotic feed additive with a particle size of 4 mm.

[0040] Table 1 Components and ratios of prebiotics used in Examples 1-4

[0041]

[0042] Example 5-11

[0043] Examples 5-11 each provide a synbiotic feed additive.

[0044] The difference between the above embodiment and embodiment 2 is that the components and proportions of probiotics are specifically shown in Table 2 below.

[0045] Table 2 Components and ratios of probiotics used in Example 2 and Examples 5-11

[0046]

[0047] Examples 12-16

[0048] Examples 12-16 each provide a synbiotic feed additive.

[0049] The difference between the above embodiment and embodiment 6 is: the type and ratio of the plant extracts are specifically shown in Table 3 below.

[0050] Table 3 Components and ratios of probiotics used in Examples 6 and 12-16

[0051] Comparative Example 1

[0052] Comparative Example 1 provides a synbiotic feed additive.

[0053] The difference between the comparative example and Example 2 is that only Poria cocos polysaccharide is used as the prebiotic. Comparative Example 2

[0054] Comparative Example 2 provides a synbiotic feed additive.

[0055] The difference between the comparative example and Example 2 is that only pullulan is used as the prebiotic. Comparative Example 3

[0056] Comparative Example 3 provides a synbiotic feed additive.

[0057] The difference between the comparative example and Example 2 is that the probiotics are Lactobacillus helveticus and Acetobacter orlanii in a weight ratio of 1:0.45. Comparative Example 4

[0058] Comparative Example 4 provides a synbiotic feed additive.

[0059] The difference between the comparative example and Example 2 is that the probiotics are Lactobacillus helveticus and Streptomyces virginiae in a weight ratio of 1:0.65. Comparative Example 5

[0060] Comparative Example 5 provides a synbiotic feed additive.

[0061] The difference between the comparative example and Example 2 is that the added amount of soybean extract is 0.

[0062] Application Example 1-16

[0063] Application Examples 1-16 each provide an animal feed.

[0064] The difference between the above application examples is that the synbiotic feed additives in the animal feed are derived from Examples 1-16 respectively.

[0065] The method for preparing animal feed provided in Application Example 1-16 comprises the following steps: adding synbiotic feed additive to commercially available feed (purchased from Shandong Jiju Biotechnology Co., Ltd.) at an addition amount of 1.2 g / kg, mixing evenly, and obtaining animal feed containing the feed additive.

[0066] Comparative Application Examples 1-5

[0067] Comparative Application Examples 1-5 each provide an animal feed.

[0068] The difference between the above application example and application example 2 is that the synbiotic feed additives in the animal feed are derived from comparative examples 1-5 respectively.

[0069] Broiler feeding trial

[0070] The animal feed provided in Application Example 1-16 and Comparative Application Example 1-5 was used to conduct a broiler feeding test. The test method is as follows:

[0071] (1) A total of 220 one-day-old AA+ white-feathered broilers were randomly divided into 22 groups, with 10 chickens in each group (half male and half female). The experimental period was 42 days.

[0072] (2) Among them, 16 groups were fed with the animal feed provided in Examples 1-16 (experimental groups 1-16); 5 groups were fed with the animal feed provided in Comparative Examples 1-5 (control groups 1-5); and 1 group was fed with commercially available feed (purchased from Shandong Jiju Biotechnology Co., Ltd.) as a blank control group. During the experiment, all conditions were basically the same, immunization and deworming were carried out according to routine procedures, feeding was carried out twice a day, food and water were freely available, feces were cleaned once a day, and the sink was scrubbed once a day.

[0073] (3) Before the experiment, the fasting body weight of the broilers was weighed; after the experiment, the fasting body weight of the broilers was weighed, and the average daily weight gain of each broiler was calculated; after the experiment, the feed consumption of each group of broilers within 42 days was recorded, and the average daily feed intake of each broiler was calculated; the feed-to-meat ratio of the broilers was comprehensively calculated, and the results are shown in Table 4 below.

[0074] Average daily weight gain of each broiler = (average weight of each group of broilers at the end of the experiment - average initial weight of each group of broilers) / 420

[0075] Average daily feed intake per broiler = feed consumption per group of broilers for 42 days / 420

[0076] Feed-to-meat ratio = average daily feed intake per broiler / average daily weight gain per broiler.

[0077] Table 4 Test results of broiler feeding experiment

[0078]

[0079] According to the test results in Table 4, the animal feed prepared by Application Example 1-16 and Comparative Application Example 1-5 and commercial animal feed were used to feed broilers for 42 days; the average daily feed intake of broilers fed with Application Example 1-16 was 101.6-108.6 g / bird, the average daily weight gain was 66.2-77.5 g / bird, and the feed-to-meat ratio was 1.40-1.53, while the average daily feed intake of broilers fed with commercial feed was 82.1-101.2 g / bird, the average daily weight gain was 38.3-58.9 g / bird, and the feed-to-meat ratio was 1.72-2.14. Obviously, the average daily feed intake and average daily weight gain of broilers fed with Application Example 1-16 were significantly higher than those of broilers fed with commercial feed, and the feed-to-meat ratio of broilers fed with Application Example 1-16 was significantly lower than that of broilers fed with commercial feed. Therefore, it is shown that the synbiotic feed additive provided in the present application can improve the intestinal microecological balance of broiler chickens, enhance the immune and disease resistance function and digestion and absorption function of broiler chickens, improve the growth performance and feed conversion rate of broiler chickens, thereby increasing the breeding efficiency of farms, and has good economic value and application prospects.

[0080] The test results of Application Example 1-4 and Comparative Application Example 1-2 show that the average daily feed intake of broilers fed with the animal feed prepared in Application Example 1-4 is 101.7-103.2g / bird, the average daily weight gain is 66.8-68.5g / bird, and the feed-to-meat ratio is 1.50-1.52; while the average daily feed intake of broilers fed with the animal feed prepared in Comparative Application Example 1-2 is 87.3-90.6g / bird, the average daily weight gain is only 46.6-48.3g / bird, and the feed-to-meat ratio is as high as 1.87-1.88. Therefore, it is explained that the present application adopts Poria cocos polysaccharide and pullulan polysaccharide with a weight ratio of 1: (0.2-0.5) as prebiotics, and the obtained synbiotic feed additive has a good use effect and a good feed conversion rate effect for broilers.

[0081] From the test results of Application Example 2, Application Examples 5-11, and Comparative Application Examples 3-4, it can be seen that the average daily feed intake of broilers fed with the animal feed prepared in Application Examples 2 and Application Examples 5-11 is 101.6-106.4 g / bird, the average daily weight gain is 67.8-72.2 g / bird, and the feed-to-meat ratio is 1.47-1.53; while the average daily feed intake of broilers fed with the animal feed prepared in Comparative Application Examples 3-4 is 96.6-97.3 g / bird, the average daily weight gain is only 49.2-52.5 g / bird, and the feed-to-meat ratio is as high as 1.85-1.96. Therefore, it is shown that the present application uses Lactobacillus helveticus, Acetobacter orlanii and Streptomyces virginia as probiotics, which can enhance the number and activity of beneficial bacteria in the intestines of broilers, promote the absorption and metabolism of nutrients in feed by broilers, and improve the feed conversion ratio of animals; further comparison shows that the feed-to-meat ratio of broilers fed with animal feed prepared in Application Examples 5-7 and Application Example 10 is only 1.47-1.48 (≤1.50), indicating that the present application further controls the weight ratio of Lactobacillus helveticus, Acetobacter orlanii and Streptomyces virginia within the range of 1: (0.4-0.5): (0.65-0.7), and the obtained synbiotic feed additive has better use effect, which can improve the feed conversion rate of broilers.

[0082] The test results of Application Examples 6 and 12-16 show that in Application Example 6, the average daily feed intake of broilers fed with animal feed made only from soybean extract was 106.4 g / bird, the average daily weight gain was 72.2 g / bird, and the feed-to-meat ratio was 1.47; in Application Examples 15-16, the average daily feed intake of broilers fed with animal feed made from soybean extract and allicin or yam extract and astragalus polysaccharide was 106.8-107.2 g / bird, the average daily weight gain was 73.4-73.5 g / bird, and the feed-to-meat ratio was 1.46; and in Application Examples 12-14, the average daily feed intake of broilers fed with animal feed made from soybean extract and astragalus polysaccharide was 108.1-108.6 g / bird (>108.0 g / bird), the average daily weight gain was 75.2-77.5 g / bird (>75.0 g / piece), and the feed-to-meat ratio is 1.40-1.44 (<1.45). Therefore, it is shown that the synbiotic feed additive prepared by further using soybean extract and astragalus polysaccharide in a weight ratio of 1: (2-4) as plant extracts in this application has a better effect and can increase the feed conversion rate of broilers.

[0083] Although the present invention has been described in detail above with general descriptions and specific embodiments, it is obvious to those skilled in the art that some modifications or improvements may be made thereto based on the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention all fall within the scope of protection claimed by the present invention.

Claims

1. A synbiotic feed additive, characterized in that: The invention comprises the following components in parts by weight: 50-100 parts of prebiotics, 10-20 parts of probiotics and 5-15 parts of plant extracts; The prebiotics are tuckahoe polysaccharide and pullulan polysaccharide in a weight ratio of 1: (0.2-0.5); the probiotics are Lactobacillus helveticus, Acetobacter orlanis and Streptomyces virginianus; the weight ratio of Lactobacillus helveticus, Acetobacter orlanis and Streptomyces virginianus is 1: (0.4-0.5): (0.65-0.7); The plant extract is selected from yam extract, soybean extract, astragalus polysaccharide and allicin.

2. The synbiotic feed additive according to claim 1, characterized in that The plant extract is a mixture of soybean extract and astragalus polysaccharide.

3. The synbiotic feed additive according to claim 1, characterized in that: The weight ratio of the soybean extract to astragalus polysaccharide is 1:(2-4).

4. The synbiotic feed additive according to claim 1, characterized in that: The synbiotic feed additive also includes 30-40 parts of soluble starch.

5. A method for preparing a synbiotic feed additive as claimed in any one of claims 1 to 4, characterized in that: The method comprises the following steps: firstly, prebiotics, probiotics and plant extracts are mixed evenly to obtain a premix; then water is added to adjust the water content of the premix to 12±2%, and a granulator is used to granulate and dry to obtain a synbiotic feed additive with a particle size of 3-5 mm.

6. An animal feed comprising the synbiotic feed additive according to any one of claims 1 to 4.

7. The animal feed according to claim 6, characterized in that The synbiotic feed additive is added in an amount of 0.8-1.5 g / kg in animal feed.

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