Preparation and application of functional characteristic reconstituted tobacco leaf

Abstract

The application discloses a kind of preparation and application of functional characteristic reconstituted tobacco, and the reconstituted tobacco includes formula essential oil, formula essential oil includes 6 parts of wood butterfly, 5 parts of salvia miltiorrhiza, 5 parts of winter-ling grass, 6 parts of liquorice, 6 parts of peppermint, 6 parts of belamcanda chinensis;Or 6 parts of mountain bean root, 6 parts of honeysuckle, 6 parts of magnolia officinalis, 6 parts of belamcanda chinensis, 6 parts of winter-ling grass, 5 parts of poria cocos.The preparation method of reconstituted tobacco includes screening tobacco powder;Take tobacco powder, smoke agent, CMC, wood pulp fiber, water, mix uniformly and place on the workbench of coating testing machine;Adjust the gap between knife roll, set temperature baking, adjust the moisture content of reconstituted tobacco, and cut into silk to form silk reconstituted tobacco;The fragrance base module containing formula essential oil is dissolved in ethanol, and is applied to silk reconstituted tobacco in the form of spray, which is functional characteristic reconstituted tobacco.The functional reconstituted tobacco prepared by the application can fully exert the unique fragrance of traditional Chinese medicine essential oil and improve the consumer experience.

Description

Technical Field

[0001] This invention relates to the field of tobacco processing technology, and in particular to the preparation and application of a functional and distinctive reconstituted tobacco leaf. Background Technology

[0002] Reconstituted tobacco is a recycled product obtained by processing tobacco raw materials such as tobacco stems, tobacco dust, and tobacco flakes. Due to the high lignin content in tobacco stems and wood pulp fibers, the resulting reconstituted tobacco has a strong woody aroma and is quite pungent. How to utilize raw materials and reconstitution processes to achieve quality reconstruction and develop high-quality reconstituted tobacco products with good synergy with cigarette products is a pressing problem that needs to be solved.

[0003] Smoking is closely related to the development of many respiratory diseases, such as chronic pharyngitis, chronic obstructive pulmonary disease, and chronic bronchitis. During combustion, cigarette smoke contains harmful substances, including oxides and free radicals, which can directly damage respiratory cells.

[0004] Chronic pharyngitis is most common among long-term smokers, manifesting as chronic inflammation of the pharyngeal mucosa, submucosa, and lymphoid tissue, characterized by a long course and high recurrence rate. Currently, treatment for chronic pharyngitis primarily involves medication, including chemical drugs, traditional Chinese medicine, and combinations of chemical drugs and traditional Chinese medicine. Compared to most drugs synthesized using modern technology, extracts from aromatic plants are more natural. When used correctly, there are no reports of harm to the human body, nor do they cause dependence or drug resistance. Natural plant essential oils are not only commonly used in tobacco flavorings, but in the field of traditional Chinese medicine research, the aromatic scents of natural herbal plants or their extracted essential oils are used to treat certain diseases through olfactory and tactile stimulation. If the aroma-enhancing and functional advantages of traditional Chinese medicine essential oils can be organically combined with reconstituted tobacco leaf development technology, the unique aroma of traditional Chinese medicine essential oils can be fully utilized, enhancing the consumer experience. Summary of the Invention

[0005] The technical problem to be solved by this invention is to provide a method for preparing and applying functional and distinctive reconstituted tobacco leaves, thereby enhancing the consumer's sensory experience.

[0006] The technical problem to be solved by the present invention is achieved through the following technical solution:

[0007] A formula essential oil, wherein the formula essential oil is a first formula essential oil or a second formula essential oil, the first formula essential oil comprising the following components by weight: 6 parts of Oroxylum indicum, 5 parts of Salvia miltiorrhiza, 5 parts of Rabdosia rubescens, 6 parts of Glycyrrhiza uralensis, 6 parts of Mentha haplocalyx, and 6 parts of Belamcanda chinensis; the second formula essential oil comprising the following components by weight: 6 parts of Sophora tonkinensis, 6 parts of Lonicera japonica, 6 parts of Magnolia officinalis, 6 parts of Belamcanda chinensis, 6 parts of Rabdosia rubescens, and 5 parts of Poria cocos.

[0008] A fragrance base module, wherein the fragrance base module is a first fragrance base module or a second fragrance base module, the first fragrance base module comprising the following components in parts by weight: 50-60% first formula essential oil, 10-15% wolfberry extract, 10-15% wild chrysanthemum extract, 5-15% bayberry extract, and 15-20% ethyl maltol; the second fragrance base module comprising the following components in parts by weight: 50-60% second formula essential oil, 10-15% astragalus extract, 10-15% loquat leaf extract, and 15-20% ethyl maltol.

[0009] Application of a formulation of essential oils or aroma base modules in the preparation of functional and distinctive reconstituted tobacco leaves.

[0010] A method for preparing a functional and distinctive reconstituted tobacco leaf includes the following steps:

[0011] (1) Place the tobacco powder raw material in a vibrating screener and pass it through a 100-mesh sieve for later use;

[0012] (2) Weigh a certain amount of formulated tobacco powder particles. Based on the mass of the tobacco powder, weigh 20% of the smoke generator, 4% of CMC, 4.5% of the wood pulp fiber, and 550% of the water. Stir and mix evenly, then place on the workbench surface of the coating tester.

[0013] (3) Adjust the gap between the cutter rollers to 1.5mm, set the temperature to 80℃, bake for 15~20min, adjust the moisture content of the reconstituted tobacco leaves to 11.7%, and then feed them into the shredder to cut into shreds. Control the shredding width to 0.9±0.1mm, curl them, and make shredded reconstituted tobacco leaves KB.

[0014] (4) Divide the filamentous reconstituted tobacco leaf KB into 3 equal parts, spray an equal amount of ethanol into one part; after dissolving the two aroma base modules in ethanol, apply them to the other two filamentous reconstituted tobacco leaves in the form of spray, which are functional characteristic reconstituted tobacco leaf samples A and B.

[0015] Preferably, in the above technical solution, in step (2), the proportion of glycerol in the smoke generator is 16% and the proportion of propylene glycol is 4%.

[0016] Preferably, in the above technical solution, in step (2), 4.5% of the wood pulp fiber is oven-dry.

[0017] Preferably, in the above technical solution, in step (4), the fragrance base module is a first fragrance base module and a second fragrance base module. The first fragrance base module includes the following components by weight: 50-60% of the first formula essential oil, 10-15% of wolfberry extract, 10-15% of wild chrysanthemum extract, 5-15% of bayberry extract, and 15-20% of ethyl maltol. The second fragrance base module includes the following components by weight: 50-60% of the second formula essential oil, 10-15% of astragalus extract, 10-15% of loquat leaf extract, and 15-20% of ethyl maltol.

[0018] Preferably, in the above technical solution, in step (4), the first formula essential oil includes the following components by weight: 6 parts of Oroxylum indicum, 5 parts of Salvia miltiorrhiza, 5 parts of Rabdosia rubescens, 6 parts of Glycyrrhiza uralensis, 6 parts of Mentha haplocalyx, and 6 parts of Belamcanda chinensis; the second formula essential oil includes the following components by weight: 6 parts of Sophora tonkinensis, 6 parts of Lonicera japonica, 6 parts of Magnolia officinalis, 6 parts of Belamcanda chinensis, 6 parts of Rabdosia rubescens, and 5 parts of Poria cocos.

[0019] Preferably, in the above technical solution, in step (4), the amount of formula essential oil applied is 4.5% of the weight of reconstituted tobacco.

[0020] The above-described technical solution of the present invention has the following beneficial effects:

[0021] The functional reconstituted tobacco prepared by this invention organically combines the aroma-enhancing and functional advantages of traditional Chinese medicine formula essential oils with reconstituted tobacco development technology. This not only fully leverages the unique aroma of the traditional Chinese medicine essential oils, enhancing the consumer experience, but also utilizes a flavoring method with filamentous reconstituted tobacco leaves. This allows for flexible modification or adjustment of the formula essential oils according to the actual needs of cigarette formulations, effectively broadening the functionality and applicability of reconstituted tobacco leaves and improving their development efficiency. Attached Figure Description

[0022] The accompanying drawings, which are incorporated in and form part of this specification, illustrate embodiments of the invention and, together with their description, serve to explain the principles of the invention.

[0023] Figure 1 This diagram illustrates the inhibitory effect of natural plant essential oils on cyclooxygenase-2.

[0024] Figure 2 These are laryngoscope images of the pharynx of rats in the control group and the model group.

[0025] Figure 3 This image shows the pharynx of a rat model of chronic pharyngitis 7 days after drug administration.

[0026] Figure 4 It is a flue gas environment testing device. Detailed Implementation

[0027] Various exemplary embodiments of the present invention will now be described in detail with reference to the accompanying drawings. It should be noted that, unless otherwise specifically stated, the relative arrangement, numerical expressions, and values ​​of the components and steps set forth in these embodiments do not limit the scope of the invention.

[0028] Unless otherwise specified, all reagents used in this application are commercially available or obtained through commercial channels, or may be prepared by referring to existing chemical methods.

[0029] Example 1: Preparation of Formulated Essential Oils

[0030] (1) Screening of natural plants:

[0031] Based on the Chinese Pharmacopoeia, the Industry License List, and relevant industry regulations, the following herbs were selected for inclusion in the candidate pool: Oroxylum indicum, Sophora tonkinensis, Scutellaria baicalensis, Salvia miltiorrhiza, Belamcanda chinensis, Lonicera japonica, Poria cocos, Magnolia officinalis, Platycodon grandiflorus, Glycyrrhiza uralensis, Mentha haplocalyx, and Rabdosia rubescens.

[0032] (2) Preparation of natural plant essential oils:

[0033] The natural plant was naturally air-dried, crushed, and placed in a CO2 supercritical extraction device for extraction. The extracted plant was then ready for use. The specific extraction process conditions are shown in Table 1.

[0034] Table 1. Preparation process and yield of natural plant essential oils

[0035]

[0036] (3) Evaluation of the inhibitory effect of natural plant essential oils on cyclooxygenase-2 (COX-2):

[0037] Cyclooxygenase (COX) typically exists in two forms: constitutively expressed COX-1 and inducibly expressed COX-2. COX-2 exhibits extremely low activity in normal tissue cells. However, when cells are stimulated by inflammation or other factors, its expression level in inflammatory cells can increase to 10-80 times the normal level, thereby participating in various pathophysiological processes and leading to inflammatory responses and tissue damage. Therefore, the search for or preparation of natural active ingredients and drugs with significant inhibitory effects on COX-2 is of great and far-reaching significance for human health.

[0038] The COX-2 inhibitory activity of the prepared natural plant essential oils was determined and screened using the Beyotime S0168 cyclooxygenase-2 (COX-2) inhibitor screening kit. A graph was plotted showing the natural plant essential oils with reliable inhibition rates. Figure 1 .

[0039] from Figure 1It can be seen that Oroxylum indicum, Sophora tonkinensis, Salvia miltiorrhiza, Belamcanda chinensis, Lonicera japonica, Poria cocos, Magnolia officinalis, Glycyrrhiza uralensis, and Rabdosia rubescens all have good inhibitory effects on cyclooxygenase-2. Platycodon grandiflorus, Mentha haplocalyx, and Astragalus membranaceus have no statistical significance due to large retesting errors.

[0040] Inhibitory effect of compound essential oils on cyclooxygenase-2 (COX-2): Based on the previous study on the inhibitory effect of single essential oils on cyclooxygenase-2, the inhibitory effect of essential oils mixed in equal proportions on COX-2 was further investigated. From the nine screened essential oils, Salvia miltiorrhiza oil and Rabdosia rubescens oil (single-drug inhibition rate > 60%) were removed. Synergistic inhibition experiments were conducted on the remaining seven essential oils, and they are numbered as follows: A-Oroxylum indicum, B-Sophora tonkinensis, C-Glycyrrhiza uralensis, D-Lonicera japonica, E-Belamcanda chinensis, F-Poria cocos, G-Magnolia officinalis.

[0041] Using a permutation and combination method, the above 7 essential oils were combined in equal proportions, and an experiment was conducted to investigate their synergistic inhibitory effect on COX-2. A total of 21 results were obtained: AB (29.27%), AC (62.69%), AD (40.93%), AE (58.31%), AF (49.33%), BD (37.58%), BE (70.42%), BF (49.16%), BG (83.98%), CD (35.13%), CE (43.99%), CF (43.42%), DE (40.00%), DF (34.62%), DG (87.95%), and EF (52.23%). CG, AG, BC, EG, and FG were considered outliers in this experiment and were not analyzed. Analysis of the valid experimental results showed that the combinations AC, AE, BE, BG, and DG had the highest synergistic inhibition rates.

[0042] Blending of Formulated Essential Oils: Based on the five compound essential oils and two single-ingredient essential oils with high synergistic inhibition rates identified in the former study, and combined with traditional Chinese medicine compatibility theory, two formulated essential oils were blended:

[0043] First formula essential oil (formula essential oil 1): 6mL of Oroxylum indicum, 5mL of Salvia miltiorrhiza, 5mL of Rabdosia rubescens, 6mL of Glycyrrhiza uralensis, 6mL of Mentha haplocalyx, and 6mL of Belamcanda chinensis.

[0044] Second formula essential oil (formula essential oil 2): ​​6mL of Sophora tonkinensis root, 6mL of honeysuckle, 6mL of Magnolia officinalis bark, 6mL of Belamcanda chinensis root, 6mL of Rabdosia rubescens root, and 5mL of Poria cocos.

[0045] Example 2: Establishment of a chronic pharyngitis model

[0046] Animal selection and grouping: SPF-grade male SD rats, weighing 180–220 g and 10 weeks old, were purchased from Shandong Jinan Pengyue Experimental Animal Breeding Co., Ltd. A total of 12 SD rats were used, with 4 rats in the control group and 8 rats in the formulated essential oil group, 4 rats per cage.

[0047] Establishment of a chronic pharyngitis model: The formulated essential oil group was given a prepared 2.5% ammonia solution, and the modeling drug was sprayed into the pharynx of rats using a laryngeal applicator, twice a day (9:00 AM and 4:00 PM, 8 hours apart), 3 puffs per application, for 4 weeks; the blank group was given an equal amount of pure water as a normal control. The chronic pharyngitis model was established, and the evaluation indicators included rat behavioral observation, rat pharyngeal laryngoscopy appearance characteristics, and rat hematological index detection - complete blood count.

[0048] (1) Behavioral observation of rats

[0049] Control group: The body condition was stable, the mental state was normal, there were no significant fluctuations in food and water intake, no signs of inflammation, and no abnormal deaths.

[0050] Model group: One week after modeling, the rats frequently scratched their mouths, increased oral secretions, increased water drinking frequency, and decreased food intake; three weeks after modeling, the rats were lethargic, their fur was rough and dull and fell out severely, their food and water intake decreased, their urine output increased, and their mouths and tongues ulcerated.

[0051] (2) Pharyngeal laryngoscopy appearance of rats

[0052] like Figure 2 As shown: In the blank group, the pharyngeal tissue of rats was light red, moist and shiny, with no excessive secretions, and no pathological phenomena such as congestion, swelling, or ulceration. In the model group, the pharyngeal tissue of rats was dark red and dark in color, with increased secretions, and large areas of acute congestion, swelling, and ulceration were observed, suggesting that this method can induce chronic pharyngitis in rats.

[0053] (3) Rat blood parameters

[0054] Table 2. Hematological parameters of rats

[0055]

[0056] Table 2 shows the results of routine blood tests in rats. Compared with the control group, the model group rats had significantly increased numbers of white blood cells, lymphocytes, and neutrophils, decreased the proportion of lymphocytes, and increased the proportion of neutrophils. This suggests that the model group rats had an inflammatory infection, and this method can induce chronic pharyngitis in rats.

[0057] Example 3: Verification of the effect of prevention and treatment of chronic pharyngitis

[0058] Experimental procedure: 20 μL of formula essential oil 1 was applied to the nostrils of all experimental rats in the model group daily (model group 1 was applied with formula essential oil 1, and model group 2 was applied with formula essential oil 2) for 7 consecutive days.

[0059] (1) Behavioral observation

[0060] After 7 days of continuous administration, the rats had normal diet and water intake, shiny fur, reduced shedding, and normal activity levels.

[0061] (2) Pharyngeal laryngoscopy appearance of rats

[0062] The color changes in the pharynx of rats were observed using a visual laryngoscope. Figure 3 .

[0063] Depend on Figure 3 It can be seen that after 7 days of intervention with the formulated essential oil on chronic pharyngitis in rats, the rats showed a significant reduction in pharyngeal secretions, a reduction in redness and swelling, and varying degrees of improvement in laryngeal vasodilation.

[0064] (3) Rat blood parameters

[0065] Table 3. Rat white blood cell (WBC) count ( (n=12)

[0066]

[0067] Note: Comparison between the model group and the normal control group: * P<0.05, ** P<0.01;

[0068] The results of routine blood tests in rats showed that, compared with the blank control group, the white blood cell counts of the model group animals were increased, indicating that the rat chronic pharyngitis model was successfully established and inflammatory cell infiltration was observed. Compared with the control group, the white blood cell counts of all treatment groups returned to normal, as shown in Table 3, suggesting that the formulated essential oil has a significant effect on clearing the throat and lungs, and improving respiratory discomfort symptoms.

[0069] Example 4: Creation of Fragrance Base Module Blending

[0070] First Fragrance Base Module (Fragrance Base Module 1): Formula essential oil 1 (by weight) 50-60%, wolfberry extract 10-15%, wild chrysanthemum extract 10-15%, bayberry extract 5-15%, ethyl maltol 15-20%.

[0071] Second fragrance base module (fragrance base module 2): Formula essential oil 2 (by weight) 50-60%, astragalus extract 10-15%, loquat leaf extract 10-15%, ethyl maltol 15-20%.

[0072] Example 5: Preparation of Functional Reconstituted Tobacco Leaves

[0073] (1) Production of reconstituted tobacco using the slurry process:

[0074] Place the tobacco powder raw material in a vibrating sieve and pass it through a 100-mesh sieve for later use;

[0075] Weigh a certain amount of formulated tobacco powder granules. Based on the weight of the tobacco powder, weigh 20% of a smoking agent (of which glycerol accounts for 16% and propylene glycol accounts for 4%), 4% of CMC, 4.5% of wood pulp fiber (octane dry), and 550% of water. After the above materials are thoroughly stirred and mixed evenly, place the slurry on the worktable surface of the coating test machine, adjust the gap between the cutter rollers to 1.5 mm to make it evenly dispersed, set the temperature to 80℃, bake for 15~20 min, adjust the moisture content of the reconstituted tobacco leaf to 11.7%, and then send it to the shredder to cut into shreds, controlling the shred width to 0.9±0.1 mm, and curl it to produce reconstituted tobacco leaf KB.

[0076] The KB filament reconstituted tobacco leaves were divided into three equal parts. One part was sprayed with an equal amount of ethanol. Two flavor base modules were weighed and dissolved in ethanol, and then applied to the other two parts of filament reconstituted tobacco leaves in the form of spray. These are functional characteristic reconstituted tobacco leaf samples A (containing the first flavor base module) and B (containing the second flavor base module). The amount of formula essential oil applied in each sample is 4.5% of the weight of the reconstituted tobacco leaves.

[0077] Sensory evaluation of functional reconstituted tobacco leaves:

[0078] The reconstituted tobacco leaves obtained above were hand-rolled into heated cigarettes. The sensory evaluation of the prepared cigarette samples was conducted in accordance with the tobacco industry's "Sensory Evaluation Requirements for Heated Cigarettes," and the results are shown in Table 4.

[0079] Table 4 Sensory Quality Evaluation of Functional Reconstituted Tobacco Leaves

[0080]

[0081] As shown in Table 4, compared with the blank, the reconstituted tobacco produced by this invention has better smoke freshness, fuller smoke concentration, moderate strength, clean aftertaste, reduced irritation, and slightly improved uniformity, showing good application prospects.

[0082] Although the present invention has been disclosed above with reference to embodiments, it is not intended to limit the present invention. Any person skilled in the art can make various different choices and modifications without departing from the spirit and scope of the present invention. Therefore, the scope of protection of the present invention is defined by the claims and their equivalents.

Claims

1. A formulated essential oil, characterized in that, The essential oil in the formula is either the first or the second formula. The first formula is composed of the following components by weight: 6 parts of Oroxylum indicum, 5 parts of Salvia miltiorrhiza, 5 parts of Rabdosia rubescens, 6 parts of Glycyrrhiza uralensis, 6 parts of Mentha haplocalyx, and 6 parts of Belamcanda chinensis. The second formula is composed of the following components by weight: 6 parts of Sophora tonkinensis, 6 parts of Lonicera japonica, 6 parts of Magnolia officinalis, 6 parts of Belamcanda chinensis, 6 parts of Rabdosia rubescens, and 5 parts of Poria cocos. The essential oil in the formula is prepared by supercritical CO2 extraction.

2. A fragrance base module, characterized in that, The fragrance base module is either a first fragrance base module or a second fragrance base module. The first fragrance base module is composed of the following components in parts by weight: 50-60% of the first formula essential oil as described in claim 1, 10-15% of wolfberry extract, 10-15% of wild chrysanthemum extract, 5-15% of bayberry extract, and 15-20% of ethyl maltol. The second fragrance base module is composed of the following components in parts by weight: 50-60% of the second formula essential oil as described in claim 1, 10-15% of astragalus extract, 10-15% of loquat leaf extract, and 15-20% of ethyl maltol.

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