Dual sample ratio microfluidic biochemical reagent disk

Abstract

The present invention relates to a dual-sample ratio microfluidic biochemical reagent disc, belonging to the technical field of biochemical analysis instruments. The dual-sample ratio microfluidic biochemical reagent disc comprises a disc base, the disc base is provided with a placement cavity, a sample addition cavity and a test group, the test group is provided with multiple groups, each of the test groups comprises a diluent quantitative cavity, a sample centrifugal packaging component, a concentration ratio mixing cavity, an arc channel, a reaction cavity and an exhaust component. The present invention has the advantage of improving test efficiency.

Description

Technical Field

[0001] The invention relates to the technical field of biochemical analysis instruments, in particular to a dual-sample ratio microfluidic biochemical reagent disc. Background Art

[0002] Biochemical analyzer is a medical in vitro diagnostic test instrument, mainly used to measure the biochemical and chemical components in clinical blood or other liquid samples. At present, biochemical analyzer is combined with microfluidics technology to achieve high-throughput biochemical analysis of trace samples.

[0003] The reagent tray used in conjunction with the biochemical analyzer is a carrier for carrying biochemical reactions. It is mainly composed of a tray base and a tray cover, wherein the tray base is usually provided with tiny channels, sample loading chambers, mixing chambers, and reaction chambers using micro-machining technology. When in use, the liquid sample to be tested is placed in the sample loading chamber in the reagent tray, and then the reagent tray is placed on the biochemical analyzer. The reagent tray is driven by the rotary motion mechanism on the biochemical analyzer to perform a rotary centrifugal motion so that the diluted sample enters each reaction chamber. The biochemical analyzer is provided with a reaction test position, and the reagent tray is driven by the rotary motion mechanism of the biochemical analyzer to rotate to the test position to complete the test. In the actual test process, the biochemical reagent tray can only test samples with a single concentration ratio each time, resulting in low test efficiency. Summary of the invention

[0004] To help improve test efficiency, the present invention provides a dual-sample ratio microfluidic biochemical reagent disk.

[0005] The dual sample ratio microfluidic biochemical reagent disk provided by the present invention adopts the following technical solutions:

[0006] A dual-sample ratio microfluidic biochemical reagent disc comprises a disc base, the disc base is circular in shape, and a placement cavity, a sample adding cavity and a test group are provided on the disc base;

[0007] The placement cavity is located at the center of the tray base and is used to place a water cup filled with diluent;

[0008] The sample adding cavity is used to accommodate the test sample;

[0009] The test group is provided with multiple groups, each of which includes a diluent quantitative chamber, a sample centrifugal packaging component, a concentration ratio mixing chamber, an arc channel, a reaction chamber and an exhaust component;

[0010] The diluent quantitative chamber is provided on the disc base and is located on a side of the placement chamber away from the sample addition chamber. The diluent quantitative chamber is connected to the placement chamber and is used for quantitative diluent.

[0011] The sample centrifugal sub-packaging component is arranged on the disc base and is located on a side of the placement cavity close to the sample adding cavity. The sample centrifugal sub-packaging component is connected to the sample adding cavity and is used to store the test sample components separated by centrifugation separately.

[0012] The concentration ratio mixing chamber is provided on the disc base, and one end is connected to the corresponding diluent quantitative chamber, and the other end is connected to the corresponding sample centrifugal dispensing assembly, and is used to mix the quantitative diluent with the quantitative centrifuged liquid sample;

[0013] The arc channel is opened on the disk base and has the same arc as the disk base, and the arc channel is connected to the mixing chamber with the corresponding concentration ratio;

[0014] The reaction chambers are provided on the disc base and are distributed in a plurality along the circumference of the corresponding arc channel, each of the reaction chambers is connected to the corresponding arc channel, and the mixed liquid sample thrown out from the concentration ratio mixing chamber under the centrifugal action enters the reaction chamber through the corresponding arc channel;

[0015] The exhaust assembly is disposed on the disk base and is connected to an end of the corresponding arc channel away from the concentration ratio mixing chamber for exhausting gas.

[0016] Preferably, the test groups are provided in two groups, wherein the volume of the diluent quantitative chamber in one of the test groups is greater than the volume of the diluent quantitative chamber in the other test group.

[0017] Preferably, the arc channels in the two test groups are respectively located on opposite sides of the placement cavity, the concentration ratio mixing chambers in the two test groups are respectively located on opposite sides of the placement cavity and between the two arc channels, the diluent quantitative chambers and the sample centrifugal packaging components in the two test groups are both located between the two concentration ratio mixing chambers, and the diluent quantitative chambers and the sample centrifugal packaging components are located on the side close to the corresponding concentration ratio mixing chamber.

[0018] Preferably, the diluent quantitative chambers in the two test groups are connected, and the diluent quantitative chambers in any one of the test groups are connected to the placement chamber. A diluent waste chamber and a first through hole are provided on the disc base. The diluent waste chamber is connected to any one of the diluent quantitative chambers in the two test groups for collecting excess diluent. The first through hole is provided on the disc base and is connected to the diluent waste chamber.

[0019] Preferably, the sample centrifugal packaging component comprises:

[0020] The sample quantification chamber is provided on the disc base and is located on the side of the placement chamber close to the sample addition chamber, and is used to quantify the liquid sample after centrifugation. The sample quantification chamber is respectively connected to the sample addition chamber and the corresponding concentration ratio mixing chamber;

[0021] The sample collection chamber is opened on the disc base and is located on the side of the corresponding sample quantitative chamber away from the placement chamber, and is used to collect red blood cells after centrifugation. The sample collection chamber is connected with the corresponding sample quantitative chamber through a linear microchannel.

[0022] Preferably, the sample addition cavity is connected with a capillary channel, one of the sample quantification cavities in the two test groups is connected with an end of the capillary channel away from the sample addition cavity, and the other sample quantification cavity is connected with the capillary channel through a connecting channel, and a sample waste liquid cavity and a second through hole are provided on the disk base, the sample waste liquid cavity is connected with any one of the sample quantification cavities in the two test groups for collecting excess sample liquid, and the second through hole is provided on the disk base and is connected with the sample waste liquid cavity.

[0023] Preferably, a limiting column is provided in one of the concentration ratio mixing chambers of the two test groups, and the limiting column is located at one end of the corresponding concentration ratio mixing chamber close to the diluent quantitative chamber, and a placement area for placing biochemical reagent freeze-dried beads is formed between the limiting column and the inner wall of the corresponding concentration ratio mixing chamber.

[0024] Preferably, the exhaust assembly comprises an exhaust hole, which is opened through the disk base and communicates with an end of the corresponding arc channel away from the concentration ratio mixing chamber.

[0025] Preferably, the exhaust assembly further comprises a waste liquid collection chamber, which is provided on the disc base and communicates with an end of the corresponding arc channel away from the concentration ratio mixing chamber.

[0026] Preferably, the sample addition chamber, the diluent quantitative chamber and the concentration ratio mixing chamber are all arc-shaped, and the arc is consistent with the arc of the circumference of the disk base.

[0027] In summary, the present invention includes the following beneficial technical effects:

[0028] When the reagent disc rotates driven by the rotating motion mechanism of the biochemical analyzer, the test sample in the sample addition chamber will be thrown into the sample centrifugal packaging component of each test group, and the sample centrifugal component will store the components of the test sample separately. Similarly, the diluent in the water cup in the placement chamber will also be thrown into the diluent quantitative chamber of each test group under the action of centrifugation; as the biochemical analyzer continues to drive the reagent disc to perform centrifugal motion, the centrifuged liquid sample enters the corresponding concentration ratio mixing chamber from the sample centrifugal packaging component. Similarly, the diluent in the diluent quantitative chamber enters the corresponding concentration ratio mixing chamber. The quantitative diluent in each test group and the liquid sample after quantitative centrifugation are evenly mixed in the concentration ratio mixing chamber, wherein the diluent in each test group The volume of the liquid release quantitative chamber is set according to the actual required concentration ratio, so that the mixed liquid samples in multiple test groups can reach different concentration ratios. After standing for a period of time, the mixed liquid sample in the concentration ratio mixing chamber will fill the capillary of the concentration ratio mixing chamber close to the corresponding arc channel, and then the reagent disk continues to rotate. The mixed liquid sample in the concentration ratio mixing chamber passes through the corresponding arc channel under the action of centrifugation and enters the corresponding reaction chambers for subsequent reactions. The gas is discharged through the corresponding exhaust component, and the mixed liquid sample finally in the reaction chamber is the test object, so that mixed liquid samples with different concentration ratios are obtained in the reaction chambers of multiple test groups, which is convenient for detecting mixed liquid samples with different concentration ratios and helps to improve test efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 It is a schematic diagram of the overall structure of an embodiment of the present invention.

[0030] Figure 2 yes Figure 1 Enlarged view of part A.

[0031] Explanation of the accompanying drawings: 1. disk base; 2. placement chamber; 3. sample addition chamber; 4. diluent quantitative chamber; 5. sample centrifugal packaging assembly; 51. sample quantitative chamber; 52. sample collection chamber; 6. concentration ratio mixing chamber; 7. arc channel; 8. reaction chamber; 9. exhaust assembly; 91. exhaust hole; 92. waste liquid collection chamber; 10. diluent waste liquid chamber; 11. first through hole; 12. capillary channel; 13. connecting channel; 14. sample waste liquid chamber; 15. second through hole; 16. limiting column; 17. placement area; 18. linear microchannel; 19. mixed waste liquid chamber; 20. third through hole; 21. first filter column; 22. second filter column; 23. diluent intermediate chamber. DETAILED DESCRIPTION

[0032] The following combination Figure 1-Figure 2 The present invention is described in further detail.

[0033] The present invention discloses a dual sample ratio microfluidic biochemical reagent disk. Figure 1 The dual-sample ratio microfluidic biochemical reagent disk includes a disk base 1, which is circular in shape and is convenient for being placed on a biochemical analyzer for centrifugal rotation; a placement cavity 2, a sample addition cavity 3 and a test group are provided on the disk base 1, wherein the placement cavity 2 is located at the center of the disk base 1 and is used for placing a water cup filled with a diluent, specifically, the diluent is usually water.

[0034] Reference Figure 1 The sample adding chamber 3 is located on one side of the placing chamber 2. The shape of the sample adding chamber 3 is arc-shaped and the arc is consistent with the circumferential arc of the disc base 1. The sample adding chamber 3 is used to accommodate the test sample. Specifically, the test sample is usually heparin anticoagulated venous whole blood, heparin anticoagulated peripheral whole blood, heparin anticoagulated plasma, serum, etc.

[0035] Reference Figure 1 There are multiple test groups on the disc base 1, each test group includes a diluent quantitative chamber 4, a sample centrifugal packaging component 5, a concentration ratio mixing chamber 6, an arc channel 7, a reaction chamber 8 and an exhaust component 9, wherein the diluent quantitative chamber 4 is opened on the disc base 1 and is located on the side of the placement chamber 2 away from the sample addition chamber 3, the diluent quantitative chamber 4 is used to quantify the diluent, and the shape of the diluent quantitative chamber 4 is also arc-shaped, and the arc is consistent with the circumferential arc of the disc base 1. The diluent quantitative chambers 4 in the multiple test groups are arranged at intervals along the circumferential direction of the placement chamber 2, and the diluent quantitative chambers 4 are connected with the placement chamber 2, so that the diluent in the water cup in the placement chamber 2 enters the diluent quantitative chamber 4 of each test group under the action of centrifugation for quantification.

[0036] Reference Figure 1 Specifically, the placement chamber 2 is connected to the diluent intermediate chamber 23 through a short passage. The diluent intermediate chamber 23 is opened on the disc base 1 and is located between the plurality of diluent quantitative chambers 4 and the placement chamber 2. The diluent quantitative chamber 4 is connected to the diluent intermediate chamber 23, thereby realizing the connection between the diluent quantitative chamber 4 and the placement chamber 2. The setting of the diluent intermediate chamber 23 helps to realize the quantitative transfer of the diluent and plays a certain buffering role.

[0037] Reference Figure 1 The sample centrifugal subpackaging component 5 is arranged on the disc base 1 and is located on the side of the placement chamber 2 close to the sample addition chamber 3. Specifically, the sample centrifugal subpackaging component 5 and the corresponding diluent quantitative chamber 4 are respectively located on the opposite sides of the placement chamber 2. The sample centrifugal subpackaging component 5 is connected to the sample addition chamber 3 and is used to separately store the test sample components separated by centrifugation.

[0038] Reference Figure 1The concentration ratio mixing chamber 6 is provided on the disk base 1. The concentration ratio mixing chamber 6 is arc-shaped, and the arc is consistent with the circumferential arc of the disk base 1. The radial distance between the concentration ratio mixing chamber 6 and the placement chamber 2 in the disk base 1 is greater than the radial distance between the sample addition chamber 3 and the placement chamber 2 in the disk base 1, which is helpful for the distribution of multiple test groups. One end of the concentration ratio mixing chamber 6 is connected to the corresponding diluent quantitative chamber 4 through a capillary, and the other end is connected to the corresponding sample centrifugal dispensing component 5 through a capillary, which is used to mix a quantitative diluent with a quantitative liquid sample after centrifugation.

[0039] Reference Figure 1 The arc channel 7 is opened on the disk base 1 and has the same arc as the circumference of the disk base 1. The arc channel 7 is connected to the corresponding concentration ratio mixing chamber 6 through a capillary, so that the mixed liquid sample in the corresponding concentration ratio mixing chamber 6 enters the corresponding arc channel 7 under the action of centrifugation. To facilitate the distribution of multiple test groups, the distance between the concentration ratio mixing chamber 6 and the placement chamber 2 in the radial direction of the disk base 1 is smaller than the distance between the arc channel 7 and the placement chamber 2 in the radial direction of the disk base 1, so that the arc channel 7 is close to the outer edge of the disk base 1.

[0040] Reference Figure 1 , the reaction chamber 8 is provided on the disc base 1 and is distributed at intervals along the circumference of the corresponding arc channel 7, each reaction chamber 8 is connected to the corresponding arc channel 7 through a small section of the channel, and the mixed liquid sample thrown out from the concentration ratio mixing chamber 6 under the action of centrifugation passes through the corresponding arc channel 7 and enters the multiple reaction chambers 8 for subsequent reaction, so that the reaction chambers 8 are rotated to the test position of the biochemical analyzer in turn to complete the test. Specifically, in actual use, the number of reaction chambers 8 can be set according to the needs of the test, so as to realize reagent discs of different specifications and make the reagent discs more optional.

[0041] Reference Figure 1 The exhaust assembly 9 is disposed on the disk base 1 and is connected to an end of the corresponding arc channel 7 away from the concentration ratio mixing chamber 6 through a channel for exhausting gas.

[0042] Taking blood as a test sample as an example, when the blood sample needs to be tested and analyzed, the blood sample is added to the sample addition chamber 3, and then the reagent tray containing the blood sample and the diluent cup is placed on the biochemical analyzer, and the reagent tray is driven to rotate by the rotating motion mechanism on the biochemical analyzer, that is, the reagent tray is centrifuged on the biochemical analyzer; during the centrifugal motion of the reagent tray, the blood sample in the sample addition chamber 3 will be thrown into the sample centrifugal packaging component 5 in each test group, and the sample centrifugal packaging component 5 will store the serum and red blood cells in the blood sample separately, among which the red blood cells are components not required in biochemical detection and analysis, while the serum or plasma are components that need to be measured in the detection and analysis; similarly, the diluent in the placement chamber 2 will also be thrown into the diluent quantitative chamber 4 in each test group under the action of centrifugation.

[0043] Then the reagent disk stops rotating, and the diluent and the centrifuged serum or plasma sample fill the capillary tube near the corresponding concentration ratio mixing chamber 6 under the action of siphon force, and then the biochemical analyzer continues to drive the reagent disk to perform centrifugal movement, and the quantitative centrifuged serum or plasma sample will enter the corresponding concentration ratio mixing chamber 6 from the sample centrifugal dispensing component 5 through the capillary tube. Similarly, the quantitative diluent in the diluent quantitative chamber 4 will enter the corresponding concentration ratio mixing chamber 6 under the action of centrifugation, and the quantitative diluent and quantitative serum or plasma sample in each test group will be evenly mixed in the concentration ratio mixing chamber 6, wherein each test group The volume of the diluent quantitative chamber 4 is set according to the actual required concentration ratio, so that the mixed liquid samples in multiple test groups can reach the required different concentration ratios; after standing for a period of time, the mixed liquid sample in the concentration ratio mixing chamber 6 will fill the capillary of the concentration ratio mixing chamber 6 close to the corresponding arc channel 7; then continue to rotate the reagent disk, and the mixed liquid sample in the concentration ratio mixing chamber 6 passes through the corresponding arc channel 7 under the action of centrifugation and enters the corresponding reaction chambers 8 for subsequent reaction, and the gas is discharged through the corresponding exhaust component 9, and finally the mixed liquid sample in the reaction chamber 8 is the test object.

[0044] Since the test sample added to the sample chamber 3 and the diluent placed in the chamber 2 in the present invention can flow to multiple test groups respectively, finally, mixed liquid samples with different concentration ratios are obtained at one time in the reaction chamber 8 of the multiple test groups on the reagent disk, which facilitates the detection of mixed liquid samples with different concentration ratios and helps to improve the test efficiency.

[0045] Reference Figure 1 There are two test groups, in which the volume of the diluent quantitative chamber 4 in one test group is larger than that in the other test group, so that the volumes of the two diluent quantitative chambers 4 are different, thereby achieving different concentration ratios of the blood sample by controlling the volume of the diluent, thereby achieving the dual sample ratio detection effect of the microfluidic biochemical reagent disk.

[0046] Reference Figure 1 In order to facilitate the reasonable distribution of the two test groups on the disc base 1, the two arc channels 7 are respectively located on the opposite sides of the placement cavity 2, and the two concentration ratio mixing cavities 6 are also respectively located on the opposite sides of the placement cavity 2, and the two concentration ratio mixing cavities 6 are located between the two arc channels 7, and the concentration ratio mixing cavity 6 and the corresponding arc channels 7 are offset in the circumferential direction of the placement cavity 2; the diluent quantitative cavity 4 and the sample centrifugal subassembly component 5 in the two test groups are both located between the two concentration ratio mixing cavities 6, and the diluent quantitative cavity 4 and the sample centrifugal subassembly component 5 are located on the side close to the corresponding concentration ratio mixing cavity 6. By reasonably arranging the positions of each chamber, there will be no interference between multiple test groups.

[0047] Reference Figure 1 The diluent quantitative chambers 4 in the two test groups are connected by a short channel, and the diluent quantitative chamber 4 in any test group is connected to the diluent intermediate chamber 23 through a capillary. Specifically, the diluent quantitative chamber 4 located in the diluent intermediate chamber 23 away from the connection with the placement chamber 2 is connected to the diluent intermediate chamber 23 through a capillary, so that the diluent can flow into the diluent intermediate chamber 23 from one end and flow out from the other end.

[0048] Reference Figure 1 , a diluent waste chamber 10 and a first through hole 11 are provided on the disk base 1. The diluent waste chamber 10 is connected to any one of the diluent quantitative chambers 4 in the two test groups through a small section of the channel. Specifically, the diluent quantitative chamber 4 that is not directly connected to the diluent intermediate chamber 23 is connected to the diluent waste chamber 10. The diluent waste chamber 10 is used to collect excess diluent. Since the two diluent quantitative chambers 4 are connected, only one diluent waste chamber 10 is required to achieve the desired effect, which helps save space. The first through hole 11 is provided through the disk base 1 and is connected to the diluent waste chamber 10 through a small section of the channel. The through hole 11 means that the disk base 1 is penetrated in the thickness direction of the disk base 1, and the first through hole 11 is connected to the outside atmosphere. When the diluent in the two diluent quantitative chambers 4 is excessive, the diluent will first enter the diluent waste chamber 10 through the channel, and the gas will be discharged through the first through hole 11.

[0049] Reference Figure 1 and Figure 2Specifically, the sample centrifugal dispensing component 5 includes a sample quantification chamber 51 and a sample collection chamber 52, wherein the sample quantification chamber 51 is opened on the disc base 1 and is located on the side of the placement chamber 2 close to the sample addition chamber 3, and the two sample quantification chambers 51 are offset from the sample addition chamber 3 in the circumferential direction of the placement chamber 2, and the sample quantification chamber 51 is close to the corresponding concentration ratio mixing chamber 6, and the sample quantification chamber 51 is connected with the sample addition chamber 3 and the corresponding concentration ratio mixing chamber 6 respectively, and the sample quantification chamber 51 is used to quantify the liquid sample after centrifugation, that is, the serum or plasma sample. The volumes of the two sample quantification chambers 51 are different, specifically, the volume of the sample quantification chamber 51 corresponding to the diluent quantification chamber 4 with a small volume is larger than the volume of the sample quantification chamber 51 corresponding to the diluent quantification chamber 4 with a large volume, so as to achieve mixed liquid samples with different concentration ratios in the two concentration ratio mixing chambers 6.

[0050] Reference Figure 1 and Figure 2 The sample collection chamber 52 is provided on the disc base 1 and is located on the side of the corresponding sample quantitative chamber 51 away from the placement chamber 2. The sample collection chamber 52 is connected with the corresponding sample quantitative chamber 51 through the linear microchannel 18. The sample collection chamber 52 is used to collect red blood cells after centrifugation. Specifically, the linear microchannel 18 is connected with the concentration ratio mixing chamber 6 in the corresponding test group through a capillary, so as to realize the connection between the sample quantitative chamber 51 and the corresponding concentration ratio mixing chamber 6.

[0051] Taking a blood sample as an example of a liquid sample for testing, when the blood sample is centrifuged, the biochemical analyzer drives the reagent disk to rotate at high speed to generate centrifugal force. Since the density of red blood cells in the blood is greater than that of serum or plasma, the red blood cells will be thrown farther under the action of centrifugation, so that the centrifuged red blood cells are in the sample collection chamber 52, and the centrifuged serum or plasma sample is in the sample quantification chamber 51, thereby achieving the effect of separating the blood and storing the components separately, so that only the serum or plasma sample can be tested.

[0052] Reference Figure 1 and Figure 2 , a capillary channel 12 is connected to the sample addition chamber 3, one of the sample quantification chambers 51 in the two test groups is connected to the end of the capillary channel 12 away from the sample addition chamber 3, and the other sample quantification chamber 51 is connected to the capillary channel 12 through the connecting channel 13, so that the two sample quantification chambers 51 are connected through the capillary channel 12 and the connecting channel 13, specifically, the sample quantification chamber 51 away from the side of the sample addition chamber 3 is connected to the end of the capillary channel 12 away from the sample addition chamber 3.

[0053] Reference Figure 1 and Figure 2, a sample waste liquid chamber 14 and a second through hole 15 are provided on the disc base 1. The sample waste liquid chamber 14 is connected to any one of the sample quantitative chambers 51 in the two test groups for collecting excess sample liquid. Specifically, the sample quantitative chamber 51 away from the sample addition chamber 3 is connected to the sample waste liquid chamber 14 through a small passage, and the sample waste liquid chamber 14 is located between the two sample quantitative chambers 51. Since the two sample quantitative chambers 51 are connected, only one sample waste liquid chamber 14 is required to achieve the desired effect, which helps save space. The second through hole 15 is provided on the disc base 1, and the second through hole 15 is connected to the outside atmosphere. The second through hole 15 is connected to the sample waste liquid chamber 14 through a small passage, and the second through hole 15 is provided to facilitate the discharge of gas.

[0054] Reference Figure 1 In order to facilitate the mixed liquid sample to fill the arc channel 7 and the reaction chamber 8, the exhaust assembly 9 includes an exhaust hole 91 and a waste liquid collection chamber 92. The exhaust hole 91 is opened through the disk base 1 and is located at the end of the corresponding arc channel 7 away from the concentration ratio mixing chamber 6. The exhaust hole 91 is connected to the outside atmosphere so that the chamber of the reaction chamber 8 is also connected to the outside atmosphere. The exhaust hole 91 is connected to the end of the corresponding arc channel 7 away from the concentration ratio mixing chamber 6 through the channel. When the mixed liquid sample enters the arc channel 7 and the corresponding reaction chamber 8, the gas can be discharged through the exhaust hole 91, so that the mixed liquid sample can be filled in each reaction chamber 8 and the arc channel 7.

[0055] Reference Figure 1 The waste liquid collection chamber 92 is also opened on the disk base 1 and is located at the end of the corresponding arc channel 7 away from the concentration ratio mixing chamber 6. The waste liquid collection chamber 92 is connected with the end of the corresponding arc channel 7 away from the concentration ratio mixing chamber 6 through a short channel. The waste liquid collection chamber 92 is used to collect the remaining mixed liquid sample.

[0056] Reference Figure 1 The concentration ratios of the two test groups in the mixing chamber 6 are connected to a mixed waste liquid chamber 19 on one side close to the placement chamber 2 through a channel. The mixed waste liquid chamber 19 is opened on the disk base 1 and is used to hold excess mixed liquid samples; the mixed waste liquid chamber 19 is connected to a third through hole 20 on one side close to the placement chamber 2 through a channel. The third through hole 20 is opened on the disk base 1 to facilitate the discharge of gas.

[0057] Reference Figure 1 and Figure 2 The first through hole 11, the second through hole 15, the third through hole 20 and the exhaust hole 91 are all filled with a molecular permeable sieve (not shown in the figure). The molecular permeable sieve allows gas to pass through but not liquid. While ensuring exhaust, it can prevent the liquid sample from leaking out of the first through hole 11, the second through hole 15, the third through hole 20 and the exhaust hole 91.

[0058] Reference Figure 1 One of the concentration ratio mixing chambers 6 in the two test groups has a limiting column 16 integrally formed therein. The limiting column 16 is located on the side of the corresponding concentration ratio mixing chamber 6 close to the diluent quantitative chamber 4. A placement area 17 for placing biochemical reagent freeze-dried beads is formed between the limiting column 16 and the inner wall of the corresponding concentration ratio mixing chamber 6. In the embodiment of the present invention, two limiting columns 16 are provided, which helps to improve the limiting effect of the biochemical reagent freeze-dried beads.

[0059] In some liver function tests and enzyme tests, the concentration ratio mixing chamber 6 needs to dissolve the biochemical reagent freeze-dried beads for pre-treatment during mixing. The placement area 17 formed between the limiting column 16 and the inner wall of the corresponding concentration ratio mixing chamber 6 is just for placing the biochemical reagent freeze-dried beads, thereby limiting the biochemical reagent freeze-dried beads, and the placement area 17 is aligned with the diluent inlet of the corresponding concentration ratio mixing chamber 6, which helps to better dissolve the biochemical reagent freeze-dried beads.

[0060] Reference Figure 1 and Figure 2 A plurality of first filter columns 21 are integrally formed on one side of the sample adding chamber 3 close to the capillary channel 12. The test sample in the sample adding chamber 3 will first pass through the plurality of first filter columns 21 before entering the capillary channel 12. The first filter columns 21 can block some hair debris or blood clots in the test sample so that they do not enter the capillary channel 12 to avoid clogging subsequent capillaries.

[0061] Reference Figure 1 and Figure 2 A plurality of second filter columns 22 are integrally formed in the linear microchannel 18 between the sample quantitative cavity 51 and the corresponding sample collection cavity 52. ​​The sample solution in the sample quantitative cavity 51 passes through the second filter column 22 and then enters the corresponding concentration ratio mixing cavity 6, which can further filter out the linear impurities in the sample, reduce the probability of capillary blockage in the reagent disk, and improve the accuracy of the test results.

[0062] The implementation principle of the embodiment of the present invention is as follows: taking blood as a test sample, when the blood sample needs to be tested and analyzed, the blood sample is added to the sample adding chamber 3, and then the reagent tray containing the blood sample and the diluent cup is placed on the biochemical analyzer, and the reagent tray is driven to rotate by the rotating motion mechanism on the biochemical analyzer, that is, the reagent tray is centrifuged on the biochemical analyzer; during the centrifugal motion of the reagent tray, the blood sample in the sample adding chamber 3 is filtered by multiple first filter columns 21 and then thrown into the sample quantitative chamber 51 and sample collection chamber 51 of each test group. In cavity 52, since the density of serum or plasma in the blood sample is lower than the density of red blood cells, the serum or plasma sample is stored in the sample quantification cavity 51, and the red blood cells are stored in the sample collection cavity 52. ​​The serum or plasma sample after centrifugation is quantified by the volume of the sample quantification cavity 51. Similarly, the diluent in the water cup in the placement cavity 2 is also thrown into the diluent intermediate cavity 23 under the action of centrifugation. Then, the diluent enters the diluent quantification cavity 4 of each test group in turn from the diluent intermediate cavity 23. The diluent in each test group is quantified by the volume of the diluent quantification cavity 4.

[0063] Then the reagent disk stops rotating, and the diluent and the serum or plasma sample after centrifugation are filled with the capillary near the corresponding concentration ratio mixing chamber 6 under the action of siphon force. Then the biochemical analyzer continues to drive the reagent disk for centrifugal movement, and the serum or plasma sample will enter the corresponding concentration ratio mixing chamber 6 from the sample quantitative chamber 51. Similarly, the diluent in the diluent quantitative chamber 4 will enter the corresponding concentration ratio mixing chamber 6 under the action of centrifugation. The quantitative diluent and quantitative serum or plasma sample in each test group are evenly mixed in the concentration ratio mixing chamber 6. Among them, the volume of the diluent quantitative chamber 4 and the sample quantitative chamber 51 in each test group is set according to the actual required concentration ratio, so that the mixed liquid samples in the two test groups can achieve different concentration ratios.

[0064] After standing for a period of time, the mixed liquid sample in the concentration ratio mixing chamber 6 will fill the capillary near the corresponding arc channel 7; then continue to rotate the reagent disk, and the mixed liquid sample in the concentration ratio mixing chamber 6 will pass through the corresponding arc channel 7 under the action of centrifugation and enter the corresponding multiple reaction chambers 8 in turn for subsequent reactions, and the gas will be discharged through the corresponding exhaust hole 91. Finally, the mixed liquid sample in the reaction chamber 8 is the test object.

[0065] In the present invention, the test sample in the sample adding chamber 3 and the diluent in the water cup placed in the chamber 2 can flow to multiple test groups respectively. The volume of the diluent quantitative chamber 4 and the sample quantitative chamber 51 in each test group is designed according to needs, and finally mixed liquid samples with different concentration ratios are obtained in the reaction chambers 8 of the two test groups at one time, realizing a double sample ratio, thereby facilitating the detection of mixed liquid samples with different concentration ratios, which helps to improve the test efficiency.

[0066] The above are all preferred embodiments of the present invention, and are not intended to limit the protection scope of the present invention. Therefore, any equivalent changes made based on the structure, shape, and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. Double sample ratio microfluidic biochemical reagent disk, characterized in that: The invention comprises a disk base (1), the disk base (1) is circular in shape, and is provided with a placement cavity (2), a sample addition cavity (3) and a test group; The placement cavity (2) is located at the center of the plate base (1) and is used to place a water cup filled with diluent; The sample adding chamber (3) is used to accommodate the test sample; The test groups are provided in a plurality of groups, each of which comprises a diluent quantitative chamber (4), a sample centrifugal dispensing component (5), a concentration ratio mixing chamber (6), an arc channel (7), a reaction chamber (8) and an exhaust component (9); The diluent quantitative chamber (4) is provided on the disc base (1) and is located on a side of the placement chamber (2) away from the sample addition chamber (3). The diluent quantitative chamber (4) and the placement chamber (2) are connected by a diluent intermediate chamber (23) for quantitatively measuring the diluent; The sample centrifugal subpackaging component (5) is arranged on the disc base (1) and is located on a side of the placement chamber (2) close to the sample addition chamber (3). The sample centrifugal subpackaging component (5) is connected to the sample addition chamber (3) and is used to store the test sample components separated by centrifugation separately. The concentration ratio mixing chamber (6) is provided on the disc base (1), and one end is connected to the corresponding diluent quantitative chamber (4), and the other end is connected to the corresponding sample centrifugal dispensing component (5), and is used for mixing a quantitative diluent with a quantitative centrifuged liquid sample; The arc channel (7) is opened on the disk base (1) and has the same arc as the circumference of the disk base (1), and the arc channel (7) is connected to the mixing chamber (6) of the corresponding concentration ratio; The reaction chamber (8) is provided on the disk base (1) and is distributed in a plurality along the circumference of the corresponding arc channel (7). Each reaction chamber (8) is connected to the corresponding arc channel (7). Under the action of centrifugation, the mixed liquid sample ejected from the concentration ratio mixing chamber (6) enters the reaction chamber (8) through the corresponding arc channel (7); The exhaust assembly (9) is disposed on the disk base (1) and is connected to an end of the corresponding arc channel (7) away from the concentration ratio mixing chamber (6) for exhausting gas; The sample addition chamber (3) is connected to a capillary channel (12); one of the sample quantification chambers (51) in the two test groups is connected to an end of the capillary channel (12) away from the sample addition chamber (3); the other sample quantification chamber (51) is connected to the capillary channel (12) via a connecting channel (13); and a plurality of first filter columns (21) are integrally formed on one side of the sample addition chamber (3) close to the capillary channel (12).

2. The dual sample ratio microfluidic biochemical reagent disk according to claim 1, characterized in that: The test groups are provided in two groups, wherein the volume of the diluent quantitative chamber (4) in one of the test groups is greater than the volume of the diluent quantitative chamber (4) in the other test group.

3. The dual sample ratio microfluidic biochemical reagent disk according to claim 2, characterized in that: The arc channels (7) in the two test groups are respectively located on opposite sides of the placement chamber (2); the concentration ratio mixing chambers (6) in the two test groups are respectively located on opposite sides of the placement chamber (2) and between the two arc channels (7); the diluent quantitative chambers (4) and the sample centrifugal sub-packaging components (5) in the two test groups are both located between the two concentration ratio mixing chambers (6); and the diluent quantitative chambers (4) and the sample centrifugal sub-packaging components (5) are located on one side close to the corresponding concentration ratio mixing chamber (6).

4. The dual sample ratio microfluidic biochemical reagent disk according to claim 2, characterized in that: The diluent quantitative chambers (4) in the two test groups are connected, and the diluent quantitative chamber (4) in any one of the test groups is connected to the placement chamber (2). The disk base (1) is provided with a diluent waste chamber (10) and a first through hole (11). The diluent waste chamber (10) is connected to any one of the diluent quantitative chambers (4) in the two test groups for collecting excess diluent. The first through hole (11) is formed through the disk base (1) and is connected to the diluent waste chamber (10).

5. The dual sample ratio microfluidic biochemical reagent disk according to claim 2, characterized in that: The sample centrifugal packaging component (5) comprises: A sample quantification chamber (51) is provided on the disc base (1) and is located on a side of the placement chamber (2) close to the sample addition chamber (3), and is used to quantify the liquid sample after centrifugation. The sample quantification chamber (51) is respectively connected to the sample addition chamber (3) and the corresponding concentration ratio mixing chamber (6); The sample collection chamber (52) is provided on the disc base (1) and is located on a side of the corresponding sample quantification chamber (51) away from the placement chamber (2), and is used to collect red blood cells after centrifugation. The sample collection chamber (52) is connected to the corresponding sample quantification chamber (51) through a linear microchannel (18).

6. The dual sample ratio microfluidic biochemical reagent disk according to claim 5, characterized in that: The disk base (1) is provided with a sample waste liquid chamber (14) and a second through hole (15). The sample waste liquid chamber (14) is connected to any one of the sample quantitative chambers (51) in the two test groups and is used to collect excess sample liquid. The second through hole (15) is formed through the disk base (1) and is connected to the sample waste liquid chamber (14).

7. The dual sample ratio microfluidic biochemical reagent disk according to claim 2, characterized in that: A limiting column (16) is arranged in one of the concentration ratio mixing chambers (6) of the two test groups. The limiting column (16) is located at one end of the corresponding concentration ratio mixing chamber (6) close to the diluent quantitative chamber (4). A placement area (17) for placing freeze-dried beads of biochemical reagents is formed between the limiting column (16) and the inner wall of the corresponding concentration ratio mixing chamber (6).

8. The dual sample ratio microfluidic biochemical reagent disk according to claim 1, characterized in that: The exhaust assembly (9) comprises an exhaust hole (91), which is opened through the disk base (1) and communicates with an end of the corresponding arc channel (7) away from the concentration ratio mixing chamber (6).

9. The dual sample ratio microfluidic biochemical reagent disk according to claim 8, characterized in that: The exhaust assembly (9) further comprises a waste liquid collection chamber (92), wherein the waste liquid collection chamber (92) is disposed on the disk base (1) and is in communication with an end of the corresponding arc channel (7) away from the concentration ratio mixing chamber (6).

10. The dual sample ratio microfluidic biochemical reagent disk according to any one of claims 1 to 9, characterized in that: The sample addition chamber (3), the diluent quantitative chamber (4) and the concentration ratio mixing chamber (6) are all arc-shaped, and the arc is consistent with the arc of the circumference of the disk base (1).

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