Application of animal Bifidobacterium lactis subspecies ZYhyy-003 in the preparation of pet products with hair beautification effect

Abstract

The present invention provides an application of animal Bifidobacterium lactis subspecies ZYhyy‑003 in preparing a pet product with hair beautification effect, belonging to the technical field of microorganisms. The present invention first proposes animal Bifidobacterium lactis subspecies ZYhyy‑003 ( Bifidobacterium animalum subsp.lactis ) is a new use in the preparation of pet products with hair beautification effects. The animal Bifidobacterium lactis subspecies ZYhyy‑003 postbiotic preparation can improve the pet's hair luminescence brightness, hair color saturation and hair breaking peak force, reduce the thickness and height of hair scales, and increase hair cystine increment.

Description

Technical Field

[0001] The invention belongs to the technical field of microorganisms, and particularly relates to application of animal Bifidobacterium lactis subspecies ZYhyy-003 in preparing pet products with hair-beautifying effects. Background Art

[0002] In recent years, with the rapid development of pet care and animal husbandry, the health of animal hair has gradually become a focus of attention. The luster, density and toughness of hair not only directly affect the appearance and health level of animals, but are also closely related to their economic value. Driven by market demand, various hair care products are emerging in an endless stream. However, most of the hair care products on the market are currently concentrated in external care agents and internal nutritional supplements, the effects of which are often short-lived and have single functions. Some products even have problems with insufficient safety, making it difficult to fully meet the market's expectations for efficient, safe, durable and diversified hair care needs.

[0003] With the rapid development of modern biotechnology, research in the field of microorganisms has provided a new solution for improving the health of animal hair. Postbiotics, as metabolites of probiotics, have become a hot research topic with great attention due to their strong stability, high safety and diverse biological activities. Postbiotics are rich in active ingredients such as short-chain fatty acids, antioxidants, and immunomodulatory factors. These ingredients can not only promote the healthy growth of hair by regulating hair follicle metabolism, but also slow down hair aging and enhance the strength of hair through antioxidant effects. In addition, postbiotics can also improve the balance of intestinal microecology, indirectly improve the overall health of animals, and further promote the improvement of hair quality.

[0004] Bifidobacterium animalis subsp. lactis is an important probiotic and its postbiotics have attracted much attention because they contain a variety of metabolically active ingredients, but there is currently no research on hair health. Summary of the invention

[0005] In view of the problems existing in the prior art, the purpose of the present invention is to provide an application of animal Bifidobacterium lactis subspecies ZYhyy-003 in the preparation of pet products with hair-beautifying effects. Based on the multi-dimensional mechanism of action of postbiotics and their potential advantages in the field of animal hair health, the present invention develops an animal Bifidobacterium lactis subspecies ZYhyy-003 postbiotic preparation with hair-beautifying function, which has important application value and market prospects in view of current market demand and technical pain points. This innovative preparation is expected to provide efficient, safe and lasting hair-beautifying solutions for the fields of pet care and animal husbandry, and contribute to promoting technological upgrading and economic benefit growth in the industry.

[0006] The objective of the present invention is achieved through the following technical solutions:

[0007] The present invention provides a collection number of CGMCC No. 31553 of animal Bifidobacterium lactis subspecies ZYhyy-003 ( Bifidobacterium animalis subsp.lactis ) in the preparation of pet products with hair-beautifying effects, characterized in that animal Bifidobacterium lactis subspecies ZYhyy-003 was deposited in the General Microbiological Center of China Culture Collection Administration on August 5, 2024, and the deposit address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing.

[0008] Furthermore, the Bifidobacterium animalis subspecies lactis ZYhyy-003 is prepared into a Bifidobacterium animalis subspecies lactis postbiotic preparation.

[0009] Furthermore, the postbiotic preparation is prepared by uniformly mixing postbiotic powder of animal Bifidobacterium lactis subspecies ZYhyy-003 and glucose, and the postbiotic powder is prepared by inactivating, concentrating and freeze-drying the bacterial liquid of animal Bifidobacterium lactis subspecies ZYhyy-003.

[0010] Furthermore, the preparation of the animal Bifidobacterium lactis subspecies ZYhyy-003 bacterial liquid comprises the following steps:

[0011] (1) Take out the frozen Bifidobacterium animalis subsp. lactis ZYhyy-003 from the -80°C refrigerator, quickly thaw it in a 37°C water bath, inoculate the strain into 5 mL of liquid MRS medium, and culture it anaerobically at 37°C for 24 to 48 hours until the bacterial solution becomes turbid;

[0012] (2) The turbid bacterial solution obtained in step (1) is inoculated on an MRS solid culture medium for activation, the activated bacteria are inoculated in a primary culture medium, and a seed solution is obtained through primary culture; the seed solution is inoculated in a secondary culture medium, and a bacterial solution is obtained through secondary culture;

[0013] (3) Centrifuge the bacterial solution at 4°C, 3000-5000 rpm for 15 min, collect the cells, wash with sterile saline, and resuspend to obtain a suspension. Adjust the concentration of the suspension to greater than 1×10 9 CFU / mL, that is, the bacterial liquid of animal Bifidobacterium lactis subspecies ZYhyy-003 was obtained.

[0014] Furthermore, in step (1), the strain inoculation amount is 0.02-0.04%.

[0015] Furthermore, in step (2), the primary culture medium and the secondary culture medium are both MRS liquid culture medium.

[0016] Furthermore, in step (2), the inoculation amount of the seed liquid inoculated into the secondary culture medium is 1% to 2%.

[0017] Furthermore, in step (2), the primary culture condition is culturing at 36-38°C for 24-48 h; the secondary culture condition is culturing at 36-38°C for 24-48 h.

[0018] Furthermore, the sterilization conditions are as follows: sterilization at 121°C for more than 30 min, applying the inactivated bacterial solution on an MRS plate, and placing it at 37°C for anaerobic culture for more than 48 h to ensure that no bacterial colonies grow.

[0019] Furthermore, the pet includes a cat, a dog, a mink, a chinchilla, a hamster, a guinea pig or a meerkat.

[0020] Furthermore, the product is one or more of the following:

[0021] (1) The product can enhance the brightness of pet hair;

[0022] (2) The product can improve the saturation of pet fur color;

[0023] (3) The product can increase the peak breaking force of pet hair

[0024] (4) The product can reduce the thickness of pet hair scales;

[0025] (5) The product can reduce the height of pet hair scales;

[0026] (6) The product can increase the cystine content in pet hair.

[0027] Strain deposit information:

[0028] Bifidobacterium animalis subsp. lactis ZYhyy-003, depository institution: General Microbiology Center of China Microbiological Culture Collection Administration (CGMCC); address: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, Postal Code: 100101; deposit date: August 5, 2024; deposit number CGMCC No. 31553; classification name: Bifidobacterium animalis subsp. lactis Bifidobacterium animalis subsp. lactis .

[0029] Compared with the prior art, the present invention has the following beneficial effects:

[0030] 1. The present invention first proposes the animal Bifidobacterium lactis subspecies ZYhyy-003 (with a deposit number of CGMCC No. 31553) Bifidobacterium animalis subsp.lactis) in the preparation of pet products with hair-beautifying effects; experimental results show that the animal Bifidobacterium lactis subspecies ZYhyy-003 postbiotic preparation described in the present application is superior to the currently developed and marketed postbiotic solid preparation with hair-beautifying effects made from Lactobacillus paracasei. Specifically, taking the animal Bifidobacterium lactis subspecies ZYhyy-003 postbiotic preparation can effectively improve the pet's hair luminescence brightness, hair color saturation and hair breaking peak force, reduce the thickness and height of hair scales, and increase the hair cystine increment. It has the characteristics of high safety and stability, easy storage and production, long shelf life and a wide range of application scenarios, and can be used as a preferred additive for pet hair-beautifying related products;

[0031] 2. The animal Bifidobacterium lactis subspecies ZYhyy-003 postbiotic preparation provided by the present invention is prepared by inactivating, concentrating and freeze-drying the bacterial liquid of animal Bifidobacterium lactis subspecies ZYhyy-003. The probiotics are inactive and long-term use will not affect the intestinal microecological balance of pets, nor cause immune imbalance or allergic reactions. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] The present invention will be further described below in conjunction with the accompanying drawings and embodiments:

[0033] Figure 1 The growth of the animal Bifidobacterium lactis subspecies ZYhyy-003 postbiotic solid preparation described in Example 2 on the MRS plate is shown;

[0034] Figure 2 The hair scores of each group of cats described in Example 3 are shown;

[0035] Figure 3 The brightness values ​​of cat hair of each group described in Example 3 are shown;

[0036] Figure 4 The saturation of cat hair in each group described in Example 3 is shown;

[0037] Figure 5 The scanning electron microscope images of the height of cat hair scales in each group described in Example 3 are shown;

[0038] Figure 6 The scanning electron microscope images of the length of cat hair scales in each group described in Example 3 are shown;

[0039] Figure 7 The scanning electron microscope images of the diameters of the cat hair scales in each group described in Example 3 are shown;

[0040] Figure 8 The height, length and diameter of each group of cat hair scales described in Example 3 are shown;

[0041] Fig. 9 The cystine increment in the hair of each group of cats described in Example 3 is shown. DETAILED DESCRIPTION

[0042] The examples are provided to better illustrate the present invention, but the present invention is not limited to the examples. Therefore, those skilled in the art may make non-essential improvements and adjustments to the implementation scheme according to the above invention content, which still fall within the protection scope of the present invention.

[0043] The endpoints and any values ​​of the ranges disclosed in this article are not limited to the precise ranges or values, and these ranges or values ​​should be understood to include values ​​close to these ranges or values. For numerical ranges, the endpoint values ​​of each range, the endpoint values ​​of each range and the individual point values, and the individual point values ​​can be combined with each other to obtain one or more new numerical ranges, which should be considered as specifically disclosed in this article.

[0044] The present invention will be described in detail below by way of examples. It should be understood that the following examples are only used to further explain and illustrate the content of the present invention in detail, and are not used to limit the present invention.

[0045] Example 1: Isolation and identification of bacterial species

[0046] 1. Source of the strain: feces of healthy pet cats in catteries and animal stray rescue stations in Yancheng District, Luohe City, Henan Province. The strain was screened and isolated from feces of healthy pet cats collected in large quantities during the period from July 1, 2023 to August 1, 2023.

[0047] 2. Screening and purification of strains

[0048] (1) Sampling: Use sterile tweezers to collect 10 g of fecal sample on a clean bench and set aside.

[0049] (2) Sample preparation:

[0050] ① Take 90 mL of sterilized saline (0.85%) and place it in a sterile conical flask, then add the sample from step (1), shake and set aside;

[0051] ② Perform gradient dilution of the dilution solution in step ①, diluting 10 -1 , 10 -2 , 10 -3 , 10 -4 and 10 -5 , numbered 1, 2, 3, 4 and 5, for use.

[0052] (3) Prepare MRS solid medium: dissolve 20.00 g of glucose, 10.00 g of peptone, 5.00 g of yeast extract, 10.00 g of beef extract, 1.00 g of Tween 80, 5.00 g of sodium acetate, 0.58 g of magnesium sulfate, 0.25 g of manganese sulfate, and 2.00 g of potassium dihydrogen phosphate in 1 L of deionized water. Add 17 g of agar and sterilize at 121°C for 15 min.

[0053] (4) Cultivation: 10 -1 , 10 -2 , 10 -3 , 10 -4 and 10 -5 The solution was spread on MRS solid medium plates using a spreading rod and cultured at 37°C for 48 h.

[0054] (5) Purification of strains: Select milky white raised colonies of different shapes and sizes for isolation and purification, and select a single colony to streak three zones on an MRS solid culture medium plate. After streaking, culture the plate at 37°C under anaerobic conditions for 48 h. Repeat the above operation for a total of three streakings to purify the pure strain.

[0055] 3. Identification of strains

[0056] The strain was cultured anaerobically at 37°C on an MRS solid medium plate for 48 h, and a single colony was picked for PCR. The primers were 16S rRNA universal primers 27F and 1492R, and the PCR reaction conditions were: 95°C pre-denaturation for 3 min; 95°C denaturation for 15 s, 60°C annealing for 15 s, 72°C extension for 90 s, 35 cycles; 72°C final extension for 5 min. The obtained 16S rRNA was subjected to BLAST analysis with the sequences of known strains on the GenBank website, and the identification result was: animal Bifidobacterium lactis subsp. Bifidobacterium animalis subsp. lactis ).

[0057] The identified strain was named Bifidobacterium animalis subsp. lactis ZYhyy-003, and the strain was sent to the General Microbiological Center of China Microbiological Culture Collection Administration for preservation. The preservation information is as follows;

[0058] Bifidobacterium animalis subsp. lactis ZYhyy-003, depository institution: General Microbiology Center of China Microbiological Culture Collection Administration (CGMCC); address: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing; deposit date: August 5, 2024; deposit number CGMCC No. 31553; classification name: Bifidobacterium animalis subsp. lactis Bifidobacterium animalis subsp.lactis .

[0059] Example 2: Preparation of animal Bifidobacterium lactis subspecies ZYhyy-003 postbiotic solid preparation

[0060] In this example, animal Bifidobacterium lactis subspecies ZYhyy-003 was used to prepare a postbiotic solid preparation, and the specific method was as follows:

[0061] (1) Prepare MRS liquid medium: dissolve 20.00 g of glucose, 10.00 g of peptone, 5.00 g of yeast extract, 10.00 g of beef extract, 1.00 g of Tween 80, 5.00 g of sodium acetate, 0.58 g of magnesium sulfate, 0.25 g of manganese sulfate, and 2.00 g of potassium dihydrogen phosphate in 1 L of deionized water and sterilize at 121°C for 15 min.

[0062] (2) Animal Bifidobacterium lactis subsp. ZYhyy-003 was activated on MRS solid medium, and the activated bacteria were inoculated into MRS liquid medium, and cultured at 37°C for 48 h to obtain seed solution; the seed solution was inoculated into MRS liquid medium at an inoculum rate of 1%, and cultured at 37°C for 48 h to obtain bacterial solution; after centrifuging the bacterial solution, the bacteria were collected, washed with sterile saline, and resuspended to obtain a suspension; the concentration of the suspension was adjusted to obtain 1.0×10 10 CFU / mL of bacterial suspension.

[0063] (3) The bacterial suspension was sterilized at 121°C for 30 min, and the inactivated bacterial suspension was spread on an MRS plate and incubated at 37°C for more than 48 hours. The sterilized bacterial suspension was freeze-dried to obtain postbiotic powder; according to the number of live bacteria in the bacterial suspension, the postbiotic powder was mixed with glucose (purchased from Shanghai MacLean Biochemical Technology Co., Ltd.) to prepare a plate containing 2.0×10-100 cells of animal Bifidobacterium lactis subsp. ZYhyy-003. 10 CFU / g of postbiotic solid preparation.

[0064] The postbiotic solid preparation was taken and coated on an MRS plate in the same manner as above, and then placed at 37°C for anaerobically culture for more than 48 hours.

[0065] The results are as follows Figure 1 As shown, there are a large number of live bacteria in the non-sterile bacterial suspension after coating, while no live bacteria are detected in the sterilized bacterial suspension and postbiotic solid preparation.

[0066] Example 3: Effect of animal Bifidobacterium lactis subsp. ZYhyy-003 postbiotic solid preparation on pet cat hair health

[0067] In order to explore the effect of the animal Bifidobacterium lactis subsp. ZYhyy-003 postbiotic solid preparation described in Example 2 on the health of pet hair, this example takes pet cats as an example and conducts the following animal experiments:

[0068] (1) Experimental groups

[0069] In this experiment, 16 pet cats from Zhongyuan Food Laboratory were randomly divided into 4 groups, 4 cats in each group, namely positive control group, negative control group, experimental group 1 and experimental group 2. The specific treatments are as follows:

[0070] Positive control group: A commercially available solid preparation of postbiotics with hair-beautifying effects (Lactobacillus paracasei ( Lactobacillus paracasei ) Postbiotics) were dissolved in 10 mL of sterile saline and administered orally once a day.

[0071] Negative control group: 10 mL of sterile saline was administered once a day.

[0072] Experimental group 1: The animal Bifidobacterium lactis subsp. ZYhyy-003 postbiotic solid preparation prepared in Example 2 was dissolved in 10 mL of sterile saline and then orally administered at a dose of 250 mg / kg body weight, once a day.

[0073] Experimental group 2: The animal Bifidobacterium lactis subsp. ZYhyy-003 postbiotic solid preparation prepared in Example 2 was dissolved in 10 mL of sterile saline and then orally administered at a dose of 500 mg / kg body weight, once a day.

[0074] All cats were vaccinated and dewormed before the experiment. The experimental environment was a 12-hour day and night cycle, with a temperature controlled at 18-26°C and a relative humidity of 30%-60%. The cattery was cleaned of feces and disinfected twice a day. Each cat was fed twice a day, 40 grams of basic feed each time. The feed was a baked staple food that met the nutritional standards of the Association of American Feed Control Officials (AAFCO, 2023) and the National Research Council (NRC, 2006). Free drinking water was provided. The week before the experiment was the adaptation period, and the following 28 days were the formal experimental period. After the experiment, the cats' hair was collected for evaluation.

[0075] All data were statistically analyzed using GraphPad Prism 10.1.2. The significance level is: ns means no significant difference, * means p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001, **** indicates p <0.0001.

[0076] This study strictly complies with international animal use guidelines and the Regulations on the Administration of Laboratory Animals in China, and has been approved by the Animal Experiment Ethics Committee of China Agricultural University (approval number: AW11104202-5-2). All experiments were conducted under the premise of minimizing animal suffering.

[0077] (2) Hair scoring

[0078] The scoring criteria for pet cat hair are shown in Table 1.

[0079]

[0080] After 28 days of feeding, the cats' hair was scored. Figure 2 As shown. The cat hair scores of experimental group 1 and experimental group 2 were significantly higher than those of the positive control group and the negative control group, and neither of them reached the greasy state of 5 points. This shows that taking the animal Bifidobacterium lactis subsp. ZYhyy-003 postbiotic solid preparation described in Example 2 can effectively improve the hair health of cats and make the hair more shiny and soft.

[0081] (3) Determination of hair brightness and saturation

[0082] This experiment was conducted in an indoor environment with normal room temperature (15-23°C) and light intensity of 100-300 lux. The brightness and saturation values ​​of animal hair were measured using a Mepda CR-400 colorimeter. The equipment needs to be blank-calibrated before measurement, and the parameters are set to an observation angle of 10°, a D65 standard light source, and a measurement aperture of 10 mm. The animals to be tested maintain a normal standing posture, and lying behavior is prohibited. The measurement sites include the head, back, left hind leg, and right hind leg. The head is defined as the midpoint area of ​​the line connecting the two ears, the back is the cross-section area of ​​the front legs and the back spine, and the hind leg part is the area where the hind leg roots are connected to the trunk. The color difference measurement needs to avoid hair with obvious color differences. Each site is measured 3 times and the average value is taken, which is recorded as the color difference value and saturation value of the hair to ensure the systematicity and accuracy of the data.

[0083] After feeding the animal Bifidobacterium lactis subsp. ZYhyy-003 postbiotic solid preparation described in Example 2, the brightness and saturation of the cat's hair were evaluated. Figure 3 As shown, the hair brightness value of experimental group 2 was significantly higher than that of the positive control group and the negative control group, and the hair brightness value of experimental group 1 was also significantly higher than that of the negative control group. This shows that the animal Bifidobacterium lactis subspecies ZYhyy-003 postbiotic solid preparation described in Example 2 can improve the brightness of cat hair, which may be by promoting fatty acid metabolism and oil secretion on the hair surface, thereby improving the glossiness of the hair.

[0084] like Figure 4As shown, the hair saturation of experimental group 2 was significantly higher than that of the positive control group, negative control group and experimental group 1. Although the hair saturation of experimental group 1 and the positive control group did not show significant differences, they were significantly higher than that of the negative control group. This shows that a high dose of animal Bifidobacterium lactis subsp. ZYhyy-003 postbiotic solid preparation can significantly improve the saturation of cat hair, possibly by optimizing lipid metabolism and enhancing skin barrier function, thereby making the hair color fuller and brighter.

[0085] (4) Measurement of peak force for breaking hair

[0086] The peak force of the hair coat was measured using a MARK-10 tensile meter, which was to obtain a single hair from the left side of the cat's buttocks and record the instantaneous tensile force when it was broken.

[0087]

[0088] The results of the peak force increment of cat hair pulling off for each group of treatments are shown in Table 2. The experimental results show that the peak force increments of hair pulling off for experimental group 1 and experimental group 2 were 9.06 and 10.44, respectively, which were significantly higher than those of the positive control group and the negative control group. This shows that the animal Bifidobacterium lactis subspecies ZYhyy-003 postbiotic solid preparation described in Example 2 can effectively improve the peak force of cat hair pulling off. The peak force of hair pulling off reflects the toughness and strength of cat hair. Hair with good toughness and high strength not only has a lighter degree of hair loss, but also has a better appearance and color. Therefore, the animal Bifidobacterium lactis subspecies ZYhyy-003 postbiotic solid preparation described in Example 2 shows significant advantages in improving hair health, and helps to enhance the stability and overall quality of hair structure.

[0089] (5) Scanning electron microscopy evaluation of pet cat hair

[0090] After 28 days of feeding, hair samples were collected from the left buttocks of each cat. When sampling, the hair in the required area was gently shaved to reduce discomfort to the animal. The hair samples were fixed on a sample tray suitable for SEM observation. The hair diameter, hair scale length and hair scale height of each sample were accurately measured using a Zeiss Sigma300 / Oxford Spectroscopy SEM. High-resolution hair surface images were recorded for subsequent analysis. The changes in hair diameter, hair scale length and hair scale thickness of cats in the control group and each experimental group were compared and analyzed. Statistical software was used to process the data to evaluate the effects of feeding different formulas on the hair microstructure, and ImageJ was used to process the photographic results.

[0091] Figure 5 , Figure 6 and Figure 7The effect of taking the animal Bifidobacterium lactis subspecies ZYhyy-003 postbiotic solid preparation described in Example 2 on the surface structure of cat hair is shown. The hair surface of the negative control group is relatively rough, with obvious cracks and scale separation. The hair surface structure of experimental group 1 and experimental group 2 is smoother, the scales are neatly arranged, and the cracks are significantly reduced. This shows that the animal Bifidobacterium lactis subspecies ZYhyy-003 postbiotic solid preparation described in Example 2 has a significant effect on improving the health of the hair surface and repairing damaged structures.

[0092] Figure 8 The effect of taking animal bifidobacterium lactis subspecies ZYhyy-003 postbiotic solid preparation on cat hair scale height, scale thickness and diameter described in Example 2 is shown. The height of hair scale of experimental group 2 is significantly lower than that of positive control group, negative control group and experimental group 1, indicating that high dose of animal bifidobacterium lactis subspecies ZYhyy-003 postbiotic solid preparation can effectively reduce hair scale height, which is conducive to improving the smoothness of hair. The hair scale length of experimental group 1 and experimental group 2 is significantly lower than that of positive control group and negative control group, indicating that animal bifidobacterium lactis subspecies ZYhyy-003 postbiotic solid preparation described in Example 2 helps to shorten the length of hair scale, improve the adhesion and uniformity of scale, and make the hair surface smoother and smoother. There is no significant difference in the hair diameter of four groups of cats, which shows that the effect of animal bifidobacterium lactis subspecies ZYhyy-003 postbiotic solid preparation described in Example 2 on hair diameter is limited, mainly acting on the repair and optimization of hair surface structure and scale.

[0093] (6) Determination of cystine increment in pet cat hair

[0094] The hair collected in the above method (5) was analyzed by an amino acid automatic analyzer (Biochrom 30 + ) to analyze the cystine content in hair.

[0095] like Fig. 9 As shown, after 28 days of feeding, the cystine content in the hair of the four groups of cats changed significantly. The cystine increment in experimental group 2 was the highest, reaching 6.4%, while the negative control group was the lowest, only 4.18%. The cystine increments in experimental group 2 and experimental group 1 were significantly higher than those in the positive control group and the negative control group. Cystine is an important component of keratin in hair, and an increase in its content usually means an improvement in hair quality and strength. Keratin is a key component of hair structure and directly affects the toughness, strength and health of hair. This shows that the animal Bifidobacterium lactis subspecies ZYhyy-003 postbiotic solid preparation described in Example 2 may improve the absorption and metabolism of nutrients by regulating the intestinal microecology, and promote the synthesis and utilization of key components such as cystine, thereby enhancing keratin synthesis in hair, promoting healthy hair growth, and improving hair quality and strength.

[0096] Finally, it should be noted that the above is only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred arrangement scheme, a person skilled in the art should understand that the technical solution of the present invention may be modified or replaced by equivalents without departing from the spirit and scope of the technical solution of the present invention.

Claims

1. Bifidobacterium animalis subspecies lactis ZYhyy-003 with a deposit number of CGMCC No. 31553 ( Bifidobacterium animalis subsp. lactis ) in the preparation of a pet product with hair beautifying effect, characterized in that: Bifidobacterium animalis subspecies lactis ZYhyy-003 was deposited in the General Microbiology Center of China Microbiological Culture Collection Administration on August 5, 2024, and the deposit address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing; The postbiotic preparation is prepared by uniformly mixing postbiotic powder of animal Bifidobacterium lactis subspecies ZYhyy-003 and glucose, and the postbiotic powder is prepared by inactivating, concentrating and freeze-drying bacterial liquid of animal Bifidobacterium lactis subspecies ZYhyy-003.

2. The use according to claim 1, characterized in that The preparation of the animal Bifidobacterium lactis subspecies ZYhyy-003 bacterial liquid comprises the following steps: (1) Take out the frozen Bifidobacterium animalis subsp. lactis ZYhyy-003 from the -80°C refrigerator, quickly thaw it in a 37°C water bath, inoculate the strain into 5 mL of liquid MRS medium, and culture it anaerobically at 37°C for 24 to 48 hours until the bacterial solution becomes turbid; (2) The turbid bacterial solution obtained in step (1) is inoculated on an MRS solid culture medium for activation, the activated bacteria are inoculated in a primary culture medium, and a seed solution is obtained through primary culture; the seed solution is inoculated in a secondary culture medium, and a bacterial solution is obtained through secondary culture; (3) Centrifuge the bacterial solution at 4°C, 3000-5000 rpm for 15 min, collect the cells, wash with sterile saline, and resuspend to obtain a suspension. Adjust the concentration of the suspension to greater than 1×10 9 CFU / mL, that is, the bacterial liquid of animal Bifidobacterium lactis subspecies ZYhyy-003 was obtained.

3. The use according to claim 2, characterized in that: In step (1), the strain inoculation amount is 0.02~0.04%.

4. The use according to claim 2, characterized in that: In step (2), the primary culture medium and the secondary culture medium are both MRS liquid culture medium.

5. The use according to claim 2, characterized in that: In step (2), the inoculation amount of the seed liquid inoculated into the secondary culture medium is 1% to 2%.

6. The use according to claim 2, characterized in that In step (2), the primary culture condition is culturing at 36-38°C for 24-48 h; the secondary culture condition is culturing at 36-38°C for 24-48 h.

7. The use according to any one of claims 1 to 6, characterized in that: The pets include cats, dogs, minks, chinchillas, hamsters, guinea pigs or meerkats.

8. The use according to any one of claims 1 to 6, characterized in that: The product is one or more of the following: (1) The product can enhance the brightness of pet hair; (2) The product can improve the saturation of pet fur color; (3) The product can increase the peak breaking force of pet hair; (4) The product can reduce the thickness of pet hair scales; (5) The product can reduce the height of pet hair scales; (6) The product can increase the cystine content in pet hair.

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