Bienzyme clostridium SE5, application and straw soil improvement productivity space-time integrated treatment method

Abstract

The invention discloses a double-enzyme clostridium sp. SE5, which is classified and named as Clostridium bifermentans, is preserved in the China General Microbiological Culture Collection Center (CGMCC), and has the preservation number of CGMCC No.1.5211. The invention also discloses a preparation method of the double-enzyme clostridium sp. The invention further discloses a straw soil improvement productivity space-time integrated treatment method based on the double-enzyme clostridium sp. SE5 and application of the double-enzyme clostridium sp. SE5 to improvement of barren soil, acid soil and saline-alkali soil. The straw is fully converted into biological energy in the same space and time, residual organic matter is converted into soil organic carbon to be immobilized in soil, the straw transportation and storage cost is reduced, the soil fertility is greatly improved, and meanwhile the biological energy in the straw is stored in the form of methane for subsequent use.

Description

Technical Field

[0001] The invention relates to the technical field of agricultural waste treatment, and in particular to a double-enzyme Clostridium SE5 and its application, and a time-space integrated treatment method for improving soil production capacity of straw. Background Art

[0002] my country's annual straw production is nearly 1 billion tons, accounting for about 25% of the world's total straw production. Straw is an agricultural byproduct and a valuable biomass resource. The Chinese government attaches great importance to the use of agricultural straw and proposes to improve the straw resource utilization system and promote the comprehensive utilization of straw, which points out the direction for further promoting the comprehensive utilization of straw. At present, my country's straw utilization methods include fertilizer (including direct return of straw to the field), energy, feed, base material and raw material.

[0003] Direct return of straw to the field is currently the main way of utilization. According to statistics, the rate of returning straw to the field exceeded 50% in 2016. Direct return of straw to the field reduces the pollution caused by straw burning on the one hand, and aims to supplement soil organic matter and improve soil quality on the other. However, according to literature data, based on the return amount of 9000kg / ha and the average humification coefficient of 0.28, the annual increase in soil organic matter content by returning straw to the field is less than 0.1%. Such a small increase in organic matter content has little effect on improving soil quality and eliminating obstacles such as salt, alkali, acid, and disease. Direct return of straw to the field will also bring a series of adverse effects on agricultural production, such as unfavorable crop seedling rooting, aggravation of pests and diseases, etc., and it is also a way of disposing of straw bioenergy that does not fully utilize it. The energy utilization of straw has also received some attention, such as biogas fermentation, which provides farmers with clean fuel while disposing of straw. However, due to factors such as high costs for straw transportation and disposal, unstable yields, difficulty in utilizing biogas slurry and residue, and minimal economic benefits, the promotion of straw biogasification is extremely difficult. Summary of the invention

[0004] Purpose of the invention: In order to overcome the shortcomings of the background technology, the first purpose of the present invention is to disclose a double-enzyme Clostridium SE5; the second purpose is to disclose a time-space integrated processing method for straw soil improvement and energy production based on double-enzyme Clostridium SE5; the third purpose is to disclose the application of double-enzyme Clostridium SE5 in improving poor soil, acid soil and saline-alkali soil.

[0005] Technical solution: The present invention discloses a double enzyme Clostridium SE5, which is classified and named Clostridium bifermentans , deposited in: General Microbiology Center of China Microbiological Culture Collection Administration, with the deposit number: CGMCC No.1.5211.

[0006] The time-space integrated processing method for improving soil production capacity by straw based on the above-mentioned double enzyme Clostridium SE5 comprises the following steps: S1, fermenting the double-enzyme Clostridium SE5 strain in a liquid culture medium to prepare a double-enzyme Clostridium SE5 anaerobic fermentation bacterial liquid; S2, using double enzyme Clostridium SE5 anaerobic fermentation liquid to cover straw and livestock and poultry manure for anaerobic fermentation to prepare a synergist; S3. Crush the straw on site and return it to the field. Add a synergist, mix it evenly with the soil, and then cover the soil surface with a film. Water the film to seal it on all sides, connect an air collection bag to the air valve on the film, collect methane, keep it for a while, then remove the film and dry the soil.

[0007] Among them, the number of viable bacteria in the anaerobic fermentation liquid in S1 is ≥ 1×10 7 CFU / mL.

[0008] Furthermore, the amount of the synergist added in S3 is 600 kg / mu.

[0009] Furthermore, the straw in S3 is staple crop straw, and the added amount is not less than 12t / mu.

[0010] Furthermore, S3 is sealed and kept for 3-6 months.

[0011] The above-mentioned dual-enzyme Clostridium SE5 is used in improving poor soil, acid soil and saline-alkali soil.

[0012] Beneficial effects: Compared with the prior art, the advantages of the present invention are: fully converting straw into bioenergy in the same space and time, converting residual organic matter into soil organic carbon and fixing it in the soil, reducing the cost of straw transportation and storage, greatly improving soil fertility, and storing the bioenergy in the straw in the form of methane for subsequent use; it can effectively improve poor soil, acid soil, saline-alkali soil and diseased soil. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 This is a schematic diagram of the implementation of ISES in Example 1; Figure 2 The effect of ISES on soil organic matter content in Example 2; Figure 3 The effect of ISES on pH of acidified soil in Example 3; Figure 4 The effect of ISES treatment on soil electrical conductivity in Example 4; Figure 5 The effect of ISES treatment on the number of Fusarium solani in soil in Example 5; Figure 6This is the effect of ISES treatment in Example 5 on the number of Fusarium oxysporum in soil. DETAILED DESCRIPTION

[0014] The technical solution of the present invention is further described below in conjunction with the accompanying drawings and embodiments. Example

[0015] like Figure 1 As shown, the spatial and temporal integrated treatment method (ISES) for improving soil production capacity by straw based on double enzyme Clostridium SE5 includes the following steps: S1, the double enzyme Clostridium SE5 strain (classified as Clostridium bifermentans , deposited in: China General Microbiological Culture Collection Center, deposit number: CGMCC No.1.5211, deposit date: July 11, 2024) fermented in liquid culture medium to produce double enzyme Clostridium SE5 anaerobic fermentation liquid; S2, using double enzyme Clostridium SE5 anaerobic fermentation liquid to cover straw and livestock and poultry manure for about 3 weeks to prepare a synergist; S3. Crush the straw on site and return it to the field. Add a synergist, mix it evenly with the soil, and then cover the soil surface with a film. Water the film to seal it on all sides, connect an air collection bag to the air valve on the film, collect methane, keep it for a while, then remove the film and dry the soil.

[0016] Among them, the number of viable bacteria in the anaerobic fermentation liquid in S1 is ≥ 1×10 7 CFU / mL; the amount of enhancer added in S3 is 600kg / mu; the straw used is staple crop straw, and the amount added is not less than 12t / mu; it should be kept for 3-6 months after sealing. If it is during May-October, it should be kept for 3 months; if it is during November-April, it needs to be treated continuously for 6 months.

[0017] Example 2: Effect of ISES on soil organic matter and methane production ISES treatment was carried out on facility soil, northern dryland soil and coastal saline-alkali soil. Specifically, 200 g of soil was placed in a 250 mL conical flask, 6% of the soil weight, pulverized wheat straw (equivalent to 12 tons per mu), and 0.3% of the synergist (equivalent to 600 kg per mu) were added and mixed thoroughly.

[0018] The double enzyme Clostridium SE5 strain is fermented in a liquid medium to prepare a double enzyme Clostridium SE5 anaerobic fermentation bacterial liquid. The synergist is composed of the double enzyme Clostridium SE5 anaerobic fermentation bacterial liquid (viable cell count ≥ 1×10 7CFU / mL) was added to a mixture of wheat straw, chicken manure and pig manure (4:3:3 ratio) and covered with film for anaerobic fermentation. Then water was added to the triangular flask to cover the soil, and the mouth of the flask was sealed with a rubber stopper with an air valve. The air valve on the rubber stopper and the air collection bag were connected with an air guide tube. The triangular flask and the air collection bag were placed at 30-35℃ and cultured for 90 days. The soil without any treatment was used as the control.

[0019] Depend on Figure 2 It can be seen that the soil organic matter content has increased significantly after treatment. Compared with the control, the organic matter content of facility soil, dryland soil and coastal saline soil increased by 83.0%, 66.2% and 52.8% respectively, indicating that ISES has a significant effect on improving soil organic matter. Improving soil organic matter will also have a certain indirect effect on physical and chemical barriers such as soil acidification, compaction and salinization. In addition, during the ISES treatment process, each triangular bottle collected a total of 530mL of methane with a concentration of 50.79%, indicating that ISES produces bioenergy methane in a good condition and can be used for subsequent energy utilization.

[0020] Example 3: Effect of ISES on the improvement of acidified soil ISES treatment was carried out on southern acidic red soil. The treatment steps were the same as those in Example 2. Figure 3 It can be seen that ISES has a significant effect on improving the pH of acidified soil. After ISES treatment, the soil pH increased by 0.56 units compared with the control.

[0021] Example 4: Desalination effect of ISES on secondary saline soil ISES treatment was carried out in the soil of the facility. Specifically, 200 g of soil was placed in a 250 mL conical flask, and 6% of the soil weight, granulated wheat straw, rice straw and corn straw (equivalent to 12 tons per mu), and 0.3% of the synergist (equivalent to 600 kg per mu) were added and mixed thoroughly. The rest of the experimental settings were the same as in Example 1.

[0022] Depend on Figure 4 It can be seen that the ISES treatments with the addition of wheat straw, rice straw and corn straw can significantly reduce the electrical conductivity of secondary saline soil caused by nitrate accumulation, which decreased by 17.8%, 32.5% and 34.7% respectively compared with the control, indicating that the ISES treatment has a significant desalination effect on secondary saline soil.

[0023] Example 5: Effect of ISES on the improvement of facility diseased soil ISES treatment was performed on the soil in the facility. The specific steps were the same as those in Example 4. Figure 5 and 6As can be seen, after the treatment, the number of two common plant pathogens, Fusarium solani and Fusarium oxysporum, in the soil decreased significantly. The number of Fusarium solani decreased by 98.2%, 98.4% and 99.1% in the ISES treatment with wheat straw, rice straw and corn stalk, respectively, and the number of Fusarium oxysporum decreased by 98.7%, 99.4% and 90.9%, respectively, indicating that the ISES treatment was effective in removing multiple pathogens in the soil.

Claims

1. A double enzyme Clostridium SE5, characterized in that: Classification Name Clostridium bifermentans , deposited in: General Microbiology Center of China Microbiological Culture Collection Administration, with the deposit number: CGMCC No.1.5211.

2. A time-space integrated processing method for improving soil production capacity by using the double enzyme Clostridium SE5 of claim 1, characterized in that: The following steps are involved: S1, fermenting the double-enzyme Clostridium SE5 strain in a liquid culture medium to prepare a double-enzyme Clostridium SE5 anaerobic fermentation bacterial liquid; S2, using double enzyme Clostridium SE5 anaerobic fermentation liquid to cover straw and livestock and poultry manure for anaerobic fermentation to prepare a synergist; S3. Crush the straw on site and return it to the field. Add a synergist, mix it evenly with the soil, and then cover the soil surface with a film. Water the film to seal it on all sides, connect an air collection bag to the air valve on the film, collect methane, keep it for a while, then remove the film and dry the soil.

3. The time-space integrated processing method for improving soil production capacity by straw according to claim 2 is characterized in that: The number of viable bacteria in the anaerobic fermentation liquid of S1 is ≥ 1×10 7 CFU / mL.

4. The time-space integrated processing method for improving soil production capacity by straw according to claim 2 is characterized in that: The amount of synergist added in S3 is 600kg / mu.

5. The time-space integrated processing method for improving soil production capacity by straw according to claim 2, characterized in that: The straw in S3 is staple crop straw, and the added amount is not less than 12t / mu.

6. The time-space integrated processing method for improving soil production capacity by straw according to claim 2, characterized in that: After sealing in S3, it can be kept for 3-6 months.

7. Use of the double enzyme Clostridium SE5 according to claim 1 in improving poor soil, acid soil and saline-alkali soil.

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