Cutting propagation method for pandanus tectorius

Abstract

The invention discloses a Pandanus tectorius cutting propagation method, and relates to the technical field of plant tissue propagation, the Pandanus tectorius cutting propagation method comprises the following steps: mixing river sand and red soil according to a volume ratio of (3-6): 1 to prepare a cutting medium, and disinfecting; a robust mother plant of pandanus tectorius is selected, semi-lignified and well-growing branches are cut to serve as cutting slips of the pandanus tectorius, and the base diameter of the cutting slips of the pandanus tectorius is 2-4 cm; the pandanus tectorius cutting slips are soaked with a naphthylacetic acid nano rooting agent; the pandanus tectorius cutting slips soaked with the rooting agent are subjected to cuttage and disinfection. The matrix formed by mixing the river sand and the red soil according to the proportion is good in drainage performance and air permeability, and the moderate humidity can be kept. The epidermis of the pandanus tectorius cutting slips is perforated, so that organic nutrition conveying from top to bottom can be slowed down, calluses are formed at the perforated positions, and the rooting rate is increased. According to the scheme, the rotting condition of the pandanus tectorius cutting slips can be effectively reduced, and the rooting rate of the pandanus tectorius cutting slips is increased.

Description

Technical Field

[0001] The invention relates to the technical field of plant tissue propagation, and in particular to a method for cutting propagation of Pandanus odoratus. Background Art

[0002] The propagation technology of Pandanus is mainly based on plant tillering. A few studies have been conducted on stem cuttings with aerial roots, and there is very little research on cutting propagation of ordinary stems without aerial roots. This is mainly because Pandanus leaves have sharp thorns that make them difficult to obtain, its branches are relatively thick, the cuttings have low lignification, the cutting medium is not suitable, and the survival rate of the cuttings is low. Summary of the invention

[0003] The main purpose of the invention is to provide a Pandanus odoratus cutting propagation method, aiming to reduce the occurrence of Pandanus odoratus cutting rot and improve the cutting rooting rate.

[0004] To achieve the above object, the method for cutting propagation of Pandanus odoratus proposed by the present invention comprises the following steps:

[0005] S10, mixing river sand and red soil in a volume ratio of (3-6):1 to prepare a cutting medium, and disinfecting the mixture;

[0006] S20, selecting a robust Pandanus mother plant, cutting semi-lignified and well-growing branches as Pandanus cuttings, and performing epidermal perforation on the Pandanus cuttings, wherein the base diameter of the Pandanus cuttings is 2-4 cm;

[0007] S30, soaking the Pandanus odoratus cuttings in step S20 with a naphthylacetic acid nano-rooting agent;

[0008] S40, implanting the Pandanus odoratus cuttings soaked in the rooting agent into the implantation medium described in step S10 and sterilizing them.

[0009] In one embodiment, in step S10, the river sand and red soil are mixed in a volume ratio of 5:1 to form a cutting medium.

[0010] In one embodiment, in step S10, the disinfection treatment includes: spraying the cutting substrate with a disinfectant.

[0011] In one embodiment, the disinfectant comprises at least one of carbendazim and potassium permanganate.

[0012] In one embodiment, in step S20, the Pandanus odoratus cuttings at least meet one of the following conditions:

[0013] Condition 1: The cutting length of the Pandanus odoratus cuttings is 20-40 cm;

[0014] Condition 2: The Pandanus cuttings retain two or more branches, of which one near the top has leaves, and the remaining length of the leaves is 2-10 cm;

[0015] Condition 3: The upper incision of the Pandanus odoratus cuttings is flat, located 1 cm above the node near the top, and the lower end is 3 cm away from the node near the bottom, cut into a smooth oblique mouth, and the upper incision is sealed with a plant wound ointment.

[0016] In one embodiment, in condition 3, when the upper incision of the Pandanus cuttings is flat, located 1 cm above the node near the top, and the lower end is 3 cm away from the node near the bottom, and is cut into a smooth oblique edge, the epidermis is punched 1 cm above the incision at the lower end of the Pandanus cuttings, the number of holes punched is 4-8, the hole diameter is 3-5 mm, and the holes are punched to 1-1.5 mm of the phloem of the Pandanus.

[0017] In one embodiment, in condition 1, the cutting length of the Pandanus odoratus cuttings is 25 cm.

[0018] In one embodiment, in condition 2, the Pandanus cuttings retain two or more branch segments, wherein a node near the top has leaves, and the remaining length of the leaves is 5 cm.

[0019] In one embodiment, in step S30: the mass concentration of the naphthacetic acid nano-rooting agent is 100-200 mg / L, and / or,

[0020] The immersion time of the naphthylacetic acid nano rooting agent is 25min-35min.

[0021] In one embodiment, in step S40:

[0022] The cutting method comprises: digging cutting holes in advance on the cutting substrate, with one plant in each pot;

[0023] The disinfection is specifically as follows: every 10 days, 50% carbendazim wettable powder is diluted 800 times and then sprayed on the cuttings, and the spraying is performed several times;

[0024] The depth of the cutting hole is 5-8 cm, and the diameter is 3-5 cm; the environmental temperature of the cutting is 25-30° C., and the humidity is 65%-75%.

[0025] The technical solution of the present invention comprises the following steps: mixing river sand and red soil in a volume ratio of (3-6):1 to form a cutting medium, and performing disinfection treatment; selecting a robust Pandanus mother plant, cutting semi-lignified and well-growing branches as Pandanus cuttings, wherein the base diameter of the Pandanus cuttings is 2-4 cm; soaking the Pandanus cuttings in a naphthaleneacetic acid nano-rooting agent; and inserting the Pandanus cuttings soaked in the rooting agent into the cutting medium and disinfecting the cuttings. The medium in which river sand and red soil are mixed in proportion not only has good drainage and air permeability, but also can maintain appropriate humidity. By soaking the cuttings in the naphthaleneacetic acid nano-rooting agent, the rooting of the cuttings can be promoted and the rooting rate can be improved. By performing epidermal perforation on the Pandanus cuttings, it is beneficial to slow down the transport of organic nutrients from top to bottom, form callus tissue at the perforation, and thus improve the rooting rate. The cutting method of this invention can effectively reduce the decay of Pandanus odoratus cuttings and improve the rooting rate of Pandanus odoratus cuttings. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without paying creative work.

[0027] Figure 1 A schematic flow chart of the Pandanus odoratus cutting propagation method provided by the present invention;

[0028] Figure 2 This is a physical picture of an embodiment of the cutting propagation of Pandanus in the present invention.

[0029] The realization of the purpose, functional features and advantages of the present invention will be further explained in conjunction with embodiments and with reference to the accompanying drawings. DETAILED DESCRIPTION

[0030] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0031] It should be noted that if the embodiments of the present invention involve directional indications (such as up, down, left, right, front, back, etc.), the directional indications are only used to explain the relative position relationship, movement status, etc. between the components in a certain specific posture. If the specific posture changes, the directional indication will also change accordingly.

[0032] In addition, if there are descriptions involving "first", "second", etc. in the embodiments of the present invention, the descriptions of "first", "second", etc. are only used for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of technical features indicated. Therefore, the features limited to "first" and "second" may explicitly or implicitly include at least one of the features. In addition, if "and / or" or "and / or" appears in the full text, its meaning includes three parallel solutions. Taking "A and / or B" as an example, it includes solution A, solution B, or solutions that satisfy both A and B. In addition, the technical solutions between the various embodiments can be combined with each other, but it must be based on the ability of ordinary technicians in this field to implement. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such combination of technical solutions does not exist and is not within the scope of protection required by the present invention.

[0033] The propagation technology of Pandanus is mainly based on plant tillering, and there is very little research on the cutting propagation of ordinary stems without aerial roots. This is mainly because Pandanus leaves have sharp thorns that make it difficult to obtain materials, its branches are relatively thick, the cuttings have low lignification, the cutting medium is not suitable, and the survival rate of the cuttings is low.

[0034] The invention provides a method for cutting propagation of Pandanus odoratus.

[0035] In one embodiment of the present invention, the method for cutting propagation of Pandanus odoratus comprises the following steps:

[0036] S10, mixing river sand and red soil in a volume ratio of (3-6):1 to prepare a cutting medium, and disinfecting the mixture;

[0037] S20, selecting a robust Pandanus mother plant, cutting semi-lignified and well-growing branches as Pandanus cuttings, and performing epidermal perforation on the Pandanus cuttings, wherein the base diameter of the Pandanus cuttings is 2-4 cm;

[0038] S30, soaking the Pandanus odoratus cuttings in step S20 with a naphthylacetic acid nano-rooting agent;

[0039] S40, implanting the Pandanus odoratus cuttings soaked in the rooting agent into the implantation medium described in step S10 and sterilizing them.

[0040] The matrix mixed with river sand and red soil in proportion not only has good drainage and air permeability, but also can maintain moderate humidity, providing a suitable growth environment for cuttings. The robust growth refers to the absence of pests and diseases, full branches and leaves, and good growth; the semi-lignified refers to the base branch diameter of 2-4cm, and the branch bark is brown-green. By soaking the cuttings in naphthaleneacetic acid nano-rooting agent, the rooting of the cuttings can be promoted and the rooting rate can be improved. By perforating the epidermis of the Pandanus cuttings, it is beneficial to slow down the transport of organic nutrients from top to bottom, form callus tissue at the perforation, and thus improve the rooting rate. Cuttings through this scheme can effectively reduce the rot of Pandanus cuttings and improve the rooting rate of Pandanus cuttings.

[0041] In an embodiment of the present invention, in step S10, the river sand and red soil are mixed in a volume ratio of 5:1 to form a cutting medium. A high proportion of river sand helps to improve the air permeability of the medium, while red soil can provide necessary nutrients and a certain adhesive force to stabilize the cuttings. Pandanus likes to grow in sandy soil, and too much red soil can easily lead to water accumulation, causing the cuttings to rot.

[0042] In the embodiment of the present invention, in step S10, the disinfection treatment includes: spraying the cutting substrate with a disinfectant to disinfect the cutting substrate. The disinfection treatment can reduce the invasion of pathogens and bacteria and improve the success rate of cutting.

[0043] In an embodiment of the present invention, the disinfectant comprises at least one of carbendazim and potassium permanganate. Carbendazim is a broad-spectrum fungicide, the main component of which is a benzimidazole compound. By interfering with the synthesis of fungal cell walls, the purpose of inhibiting fungal growth and reproduction is achieved. Potassium permanganate destroys the cell structure of microorganisms by releasing active oxygen, thereby playing a bactericidal role.

[0044] In an embodiment of the present invention, in step S20, the cutting length of the Pandanus odoratus cuttings is 20-40 cm. Keeping a certain length is conducive to ensuring the nutrients and endogenous hormone levels required for rooting of the cuttings, and is conducive to the water supply of the cuttings. Preferably, the cutting length of the Pandanus odoratus cuttings is 25 cm.

[0045] In an embodiment of the present invention, in step S20, the Pandanus cuttings retain two or more branches, wherein one of the branches near the top has leaves, and the length of the leaves retained is 2-10 cm. Retaining the truncated leaves is conducive to driving the transpiration of the cuttings, retaining the activity of the cuttings, and promoting nutrient conversion. Retaining two nodes, inserting the matrix near the bottom, retaining the leaves near the top, and retaining the complete structure of the middle cuttings is conducive to the normal growth function of the cuttings and promoting the rooting and germination of the cuttings. Preferably, in one embodiment of the present invention, the retained length of the leaves is 5 cm.

[0046] In an embodiment of the present invention, in step S20, the upper incision of the Pandanus cuttings is a flat mouth, located 1cm above a near top section, and the lower end is cut into a smooth oblique mouth at 3cm from a near bottom section, and the upper incision is sealed with a plant wound smear. The oblique mouth is set to increase the contact area between the Pandanus cuttings and the soil, and the inclined surface can better absorb water and nutrients, and is also conducive to the accumulation of rooting materials and the beginning of the rooting process. The oblique mouth shape helps the cut surface of the cuttings to fully contact with the soil, reduces the occupied space of the air, thereby reducing water evaporation, and keeping the vitality of the cuttings. In addition, the oblique mouth can also provide a larger area for the root primordium to form, improve the success rate of rooting.

[0047] In an embodiment of the present invention, when the upper incision of the Pandanus cuttings is flat, located 1 cm above the node near the top, and the lower end is 3 cm away from the node near the bottom, and is cut into a smooth oblique mouth, the epidermis is punched 1 cm above the incision at the lower end of the Pandanus cuttings, the number of holes is 4-8, the hole diameter is 3-5 mm, and the holes are punched to 1-1.5 mm of the phloem of the Pandanus. The epidermis punching is conducive to slowing down the organic nutrition transportation from top to bottom, forming callus tissue at the punching place, thereby improving the rooting rate.

[0048] In the embodiment of the present invention, in step S30: the mass concentration of the NANO rooting agent is 100-200 mg / L. Within this concentration range, the NANO rooting agent can effectively stimulate the rooting process of the cuttings, accelerate the rooting speed, ensure a large number of roots and a well-developed root system, and improve the rooting quality.

[0049] In the embodiment of the present invention, in step S30, the naphthaleneacetic acid nano rooting agent is soaked for 25-35 minutes. In this time period, rooting can be promoted without damaging the cuttings due to soaking for too long, thus ensuring the healthy growth of the root system.

[0050] In an embodiment of the present invention, in step S40:

[0051] The cutting method comprises: digging cutting holes in advance on the cutting substrate, with one plant in each pot;

[0052] The disinfection is specifically as follows: every 10 days, 50% carbendazim wettable powder is diluted 800 times and then sprayed on the cuttings, and the spraying is performed several times;

[0053] The depth of the cutting hole is 5-8 cm, and the diameter is 3-5 cm; the environmental temperature of the cutting is 25-30° C., and the humidity is 65%-75%.

[0054] Regular disinfection with carbendazim can effectively prevent the cuttings from being attacked by bacteria and fungi and ensure the health of the cuttings. The depth and diameter of the cutting holes are conducive to the development of the root system, which helps the cuttings adapt to the new environment faster and start growing. Suitable environmental conditions and good disinfection measures can help improve the survival rate of cuttings, thereby improving the breeding efficiency.

[0055] Example 1

[0056] The present embodiment provides a method for cutting propagation of Pandanus odoratus, comprising the following steps:

[0057] S10, mix river sand and red soil in a volume ratio of 5:1 to prepare a cutting medium, and use 800 times solution of 50% carbendazim wettable powder to thoroughly irrigate the cutting medium for disinfection; the red soil is sieved in advance, and the mesh size is 2mm; the particle size of the river sand is ≤2mm;

[0058] S20, select a robust Pandanus mother plant, cut semi-lignified and well-growing branches as Pandanus cuttings, the base diameter of the Pandanus cuttings is 2-4cm, and the length is 25cm; the Pandanus cuttings retain two or more branches, wherein one section near the top has leaves, and the length of the leaves retained is 5cm; the upper incision is flat, located 1cm above the section, and the lower end is 3cm away from the section, cut into a smooth oblique mouth; the epidermis is punched 1cm above the incision at the lower end of the Pandanus cuttings, the number of holes is 4-8, the aperture is 3-5mm, and the holes are punched to 1-1.5mm of the Pandanus phloem; the upper incision is sealed with a plant wound smear;

[0059] S30, soaking the Pandanus officinalis cuttings in step S20 with a 150 mg / L naphthaleneacetic acid nano-rooting agent for 30 min;

[0060] S40, dig cutting holes in advance, with a depth of 5-8 cm and a diameter of 4 cm, and cut the Pandanus odoratus cuttings treated with the rooting agent into the cutting medium described in step S10, one plant per pot, the cutting environment temperature is 25-30°C, the humidity is 65%-75%, and the cuttings are sprayed with 50% carbendazim wettable powder diluted 800 times every 10 days, and the spraying is continuous 3 times.

[0061] Example 2

[0062] Compared with Example 1, the difference of this example is that in S10, 2% potassium permanganate is sprayed on the cutting medium for disinfection.

[0063] Comparative Example 1

[0064] Compared with Example 1, the difference of this comparative example is that: the length of the Pandanus odoratus cuttings in S20 is 40 cm;

[0065] Comparative Example 2

[0066] Compared with Example 1, the difference of this comparative example is that in S30, the Pandanus odoratus cuttings in step S20 are soaked with ABT1 rooting powder with a concentration of 150 mg / L for 30 minutes.

[0067] Comparative Example 3

[0068] Compared with Example 1, the difference of this comparative example is that no punching process is performed in S20.

[0069] Comparative Example 4

[0070] Compared with Example 1, the difference of this comparative example is that in S10, river sand and red soil are mixed in a volume ratio of 3:1 to form a cutting medium.

[0071] The rooting conditions of the cuttings of Examples 1 and 2 and Comparative Examples 1-4 were recorded, and the rooting rates are shown in Table 1.

[0072] Table 1 Rooting rate of Examples 1, 2 and Comparative Examples 1-4

[0073] Semi-lignified rooting rate Example 1 86％ Example 2 83％ Comparative Example 1 55％ Comparative Example 2 52％ Comparative Example 3 61％ Comparative Example 4 49％

[0074] As shown in Table 1, the rooting rates of Examples 1 and 2 are both higher than 80%. In Comparative Example 1, the length of the Pandanus cuttings is changed, and the leaves are reduced after the cuttings are cut, the transpiration is weakened, and there is no root for nutrient absorption. The cuttings are used for nutrient transformation and the retained leaves for nutrient absorption, but the cuttings are too long, which will cause the originally weak transpiration to fail to proceed normally, and the nutrients cannot be normally transformed and transported, resulting in the inhibition of rooting of the cuttings. Comparative Example 2 changes the type of rooting powder. Since different types of rooting powders contain different hormone components and concentrations, hormones such as indoleacetic acid (IAA), indolebutyric acid (IBA) and naphthaleneacetic acid (NAA) play a key role in promoting plant rooting. Different plants or cuttings have different sensitivity to hormones, and selecting naphthaleneacetic acid nano rooting agent can significantly improve the rooting rate and rooting quality. Comparative Example 3 did not perform ring barking treatment, and the rooting rate decreased by 25%. The perforation of the epidermis was conducive to slowing down the organic nutrient transport from top to bottom, forming callus at the perforation, making it easier for nutrients and hormones to reach the rooting part of the cuttings, thereby improving the rooting rate. Comparative Example 4 changed the ratio of river sand and red soil, and the rooting rate decreased by 43%. It is inferred that this is because Pandanus likes to grow in sandy soil, and the excessive proportion of red soil in the soil is easy to cause waterlogging, causing the cuttings to rot.

[0075] The above description is only an exemplary embodiment of the present invention, and does not limit the patent scope of the present invention. All equivalent structural changes made by using the contents of the present invention specification and drawings under the technical concept of the present invention, or directly / indirectly applied in other related technical fields are included in the patent protection scope of the present invention.

Claims

1. A method for cutting propagation of Pandanus odoratus, characterized in that: The following steps are involved: S10, mixing river sand and red soil in a volume ratio of (3-6):1 to prepare a cutting medium, and disinfecting the mixture; S20, selecting a robust Pandanus mother plant, cutting semi-lignified and well-growing branches as Pandanus cuttings, and performing epidermal perforation on the Pandanus cuttings, wherein the base diameter of the Pandanus cuttings is 2-4 cm; S30, soaking the Pandanus odoratus cuttings in step S20 with a naphthylacetic acid nano-rooting agent; S40, implanting the Pandanus odoratus cuttings soaked in the rooting agent into the implantation medium described in step S10 and sterilizing them.

2. The method for cutting propagation of Pandanus odoratus as claimed in claim 1, characterized in that: In step S10, the river sand and red soil are mixed in a volume ratio of 5:1 to form a cutting medium.

3. The method for cutting propagation of Pandanus odoratus as claimed in claim 1, characterized in that: In step S10, the disinfection treatment includes: spraying the cutting matrix with a disinfectant to disinfect it.

4. The method for cutting propagation of Pandanus odoratus as claimed in claim 3, characterized in that: The disinfectant comprises at least one of carbendazim and potassium permanganate.

5. The method for cutting propagation of Pandanus odoratus as claimed in claim 1, characterized in that: In step S20, the Pandanus odoratus cuttings at least meet one of the following conditions: Condition 1: The cutting length of the Pandanus odoratus cuttings is 20-40 cm; Condition 2: The Pandanus cuttings retain two or more branches, of which one near the top has leaves, and the remaining length of the leaves is 2-10 cm; Condition 3: The upper incision of the Pandanus odoratus cuttings is flat, located 1 cm above the node near the top, and the lower end is 3 cm away from the node near the bottom, cut into a smooth oblique mouth, and the upper incision is sealed with a plant wound ointment.

6. The method for cutting propagation of Pandanus odoratus as claimed in claim 5, characterized in that: In condition 3, when the upper incision of the Pandanus cuttings is flat, located 1 cm above the node near the top, and the lower end is 3 cm away from the node near the bottom, and is cut into a smooth oblique edge, the epidermis is punched 1 cm above the incision at the lower end of the Pandanus cuttings, with 4-8 holes and a hole diameter of 3-5 mm, and the holes are punched to 1-1.5 mm of the phloem of the Pandanus.

7. The method for cutting propagation of Pandanus odoratus as claimed in claim 5, characterized in that: In condition 1, the cutting length of the Pandanus odoratus cuttings is 25 cm.

8. The method for cutting propagation of Pandanus odoratus as claimed in claim 5, characterized in that: In condition 2, the Pandanus odoratus cuttings retain two or more branch segments, of which the node near the top has leaves, and the remaining length of the leaves is 5 cm.

9. The method for cutting propagation of Pandanus odoratus according to claim 1, characterized in that: In step S30: the mass concentration of the naphthylacetic acid nano-rooting agent is 100-200 mg / L, and / or, The immersion time of the naphthylacetic acid nano rooting agent is 25min-35min.

10. The method for cutting propagation of Pandanus odoratus according to claim 1, characterized in that: In step S40: The cutting method comprises: digging cutting holes in advance on the cutting substrate, with one plant in each pot; The disinfection is specifically as follows: every 10 days, 50% carbendazim wettable powder is diluted 800 times and then sprayed on the cuttings, and the spraying is performed several times; The depth of the cutting hole is 5-8 cm, and the diameter is 3-5 cm; the environmental temperature of the cutting is 25-30° C., and the humidity is 65%-75%.

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