A convenient rice seedling salt-tolerant root activity monitoring device and a use method thereof

Abstract

The application relates to the technical field of monitoring of salt-tolerant root activity of rice seedlings, and discloses a convenient monitoring device for salt-tolerant root activity of rice seedlings, which comprises a culture pool and a detection pool, the middle of the culture pool is fixedly connected with a partition frame, the top of the partition frame is fixedly connected with a water-proof ring, the water-proof ring is movably placed above a culture plate, five groups of culture openings are formed in the left and right sides of the culture plate, culture nets are fixedly installed in the middle of the culture openings, and the bottom of the culture plate is movably connected with a separate auxiliary plate. The application utilizes the adsorption capacity of the surface of rice root systems, obtains monitoring and recording data of root activity of the same group of rice seeds at different stages under the premise that irreversible damage is not caused to the rice root systems, the isolation barrels on the separate auxiliary plates are used to isolate the rice root systems, the root systems are prevented from being entangled, root activity data of multiple groups of rice seeds is obtained at one time, and the monitoring efficiency is greatly improved.

Description

Technical Field

[0001] This invention relates to the field of monitoring salt-tolerant root activity in rice seedlings, specifically a convenient device for monitoring salt-tolerant root activity in rice seedlings and its usage method. Background Technology

[0002] Global soil salinization is becoming increasingly serious and is one of the common abiotic stresses affecting crop growth and yield. As an important field food crop, rice is moderately sensitive to salt stress. Studying the salt tolerance of rice varieties and cultivating salt-tolerant varieties can improve salt tolerance while maintaining and stabilizing yields, which has important practical application value.

[0003] Rice seeds often exhibit various salt damage symptoms when subjected to salt stress. Among these, rice root activity has a strong correlation with the salt tolerance of rice varieties. However, current methods for detecting rice roots are mostly irreversible operations, making it difficult to monitor and record root activity at different stages of the same group of rice seeds. Furthermore, there is a lack of devices that facilitate rapid detection of root activity, resulting in low efficiency in identifying and detecting the root activity of salt-tolerant varieties after salt stress. Summary of the Invention

[0004] This addresses the shortcomings of existing rice seedling root vigor monitoring devices and methods described in the background section during use.

[0005] This invention provides the following technical solution: a convenient device for monitoring the salt-tolerant root vigor of rice seedlings, comprising a culture tank and a detection tank. A partition is fixedly connected to the middle of the culture tank, and a water-proof ring is fixedly connected to the top of the partition. A culture plate is movably placed above the water-proof ring. Five sets of cultivation openings are provided on both sides of the culture plate. A cultivation net is fixedly installed in the middle of each cultivation opening. A partition plate is movably connected to the bottom of the culture plate, positioned between the culture plate and the water-proof ring. Fixing buckles are movably connected to both sides of the partition plate. Grooves matching the fixing buckles are provided on both sides of the culture plate. The partition plate is positioned at each set of cultivation openings. An isolation cylinder is fixedly connected to the corresponding position at the bottom, and an isolation ring is fixedly connected to the top. Side ear supports are fixedly connected to the top of the front and back of the detection pool. A one-way toothed groove is fixedly connected to the middle of the side ear supports. A rotating shaft is movably sleeved in the middle of the one-way toothed groove. A one-way knob is fixedly connected to the front of the rotating shaft, and an adjusting tooth is fixedly connected to the back of the rotating shaft. An isolation plate is placed inside the detection pool. A sealing ring is fixedly installed on the top of the isolation plate corresponding to the position of the isolation cylinder. A cuvette slot is fixedly connected inside the sealing ring and the isolation plate. An isolation frame is fixedly connected to the front and back of the isolation plate, and a rotating tooth is provided in the middle of the isolation frame.

[0006] Preferably, the isolation cylinder is vertically continuous.

[0007] Preferably, the detection pool is provided with a partition in the middle, the top surface of the detection pool and the partition wall is provided with a transverse slide rail groove, and the bottom surface of the partition plate is provided with a slide rail.

[0008] Preferably, the adjusting tooth meshes with the rotating tooth, and the rotating shaft is provided with meshing teeth that match the unidirectional tooth groove.

[0009] Preferably, the width of the adjusting tooth is greater than the width of the meshing tooth on the rotating shaft.

[0010] A convenient method for using a rice seedling salt-tolerant root vigor monitoring device includes the following operating steps: This invention has the following beneficial effects:

[0011] S1. Place conventional culture medium and culture medium with the experimentally set salinity on both sides of the partition in the culture tank until the liquid surface contacts the culture net.

[0012] S2. After attaching the partition plate to the bottom of the culture plate and securing it with the fasteners, place it in the culture tank as a whole.

[0013] S3. Place the five different rice plants on the five culture nets respectively and wait for them to take root;

[0014] S4. During testing, remove the culture plate and the partition plate as a whole from the culture tank and place them into the testing tank, so that the slide rail on the bottom surface of the partition plate engages with the slide rail groove on the top surface of the testing tank.

[0015] S5. Pour methylene blue solution into the water tank until the solution falls into the test pool and completely submerges the rice roots, then let it stand for minutes.

[0016] S6. Open the drain outlet at the bottom of the detection pool to drain the internal methylene blue solution, then close the drain outlet.

[0017] S7. Pour clean water into the watering tank until the water completely submerges the rice roots;

[0018] S8. Shake the culture plate and the partition plate left and right along the slide rail to allow the rice roots to be fully rinsed in clean water, and then open the drain of the test pool to discharge them.

[0019] S9. Repeat step SS four to five times;

[0020] S10. After removing the culture plate and the separator plate, attach the cuvette to the cuvette slot and place the culture plate and the separator plate back into the center of the detection cell.

[0021] S11. Push the one-way knob inward until the teeth on the rotating shaft mesh with the one-way tooth groove. Rotate the rotating shaft to pull the isolation frame and isolation plate up until the sealing ring presses against the bottom surface of the isolation cylinder.

[0022] S12. Pour calcium chloride solution into the water tank until it completely submerges the rice roots, and let it stand for 5-10 minutes.

[0023] S13. Open the drain outlet of the test pool, pull the one-way knob outward to disengage the teeth on the rotating shaft from the one-way groove, and then slowly reverse the one-way knob to make the isolation plate fall back.

[0024] S14. After the excess solution flows out of the drain, remove the cuvettes from the cuvette slots in sequence and place them in the spectrophotometer for measurement and recording.

[0025] S15. Place the culture plate and separator plate containing the tested rice seedlings back into the culture tank for continued cultivation.

[0026] 1. This invention utilizes the adsorption capacity of rice root surfaces to obtain and record root vitality data of the same group of rice seeds at different stages of different periods without causing irreversible damage to the rice root system. The isolation tubes on the partition plates of each group isolate the rice roots of each group, avoiding root entanglement, while obtaining root vitality data of multiple groups of rice seeds at one time, which greatly improves the monitoring efficiency.

[0027] 2. This invention utilizes the adsorption capacity of rice root surfaces to immerse vigorous roots in a methylene blue solution, adsorbing methylene blue ions and staining them blue. After rinsing, the roots are immersed in a calcium chloride solution. The calcium ions and positively charged methylene blue ions undergo an exchange reaction, allowing the methylene blue ions on the root surface to enter the calcium chloride solution. The color depth of the calcium chloride solution is then used to colorimetrically detect differences in rice root vigor. This allows for the acquisition of root vigor data without causing irreversible damage to the rice roots, enabling the monitoring and recording of root vigor at different stages from the same group of rice seeds.

[0028] 3. This invention separates the rice roots of each group by attaching a partition plate under the culture plate and using the isolation tubes on each partition plate. This not only avoids the roots of different groups from tangling with each other, thus affecting the accuracy of subsequent detection, but also facilitates the separate collection of methylene blue ions adsorbed by the roots of each group and the control group when immersed in calcium chloride solution. This makes it convenient to detect the root vitality status of multiple groups of different rice varieties at one time, greatly improving the detection efficiency.

[0029] 4. By setting a sliding rail groove on the detection pool, the present invention enables the roots of all experimental groups and control groups to be thoroughly rinsed in clean water by sliding the partition plate left and right during the rinsing step. This greatly reduces the complexity of the operation of multiple experimental groups, making the operation of detecting salt-tolerant root vigor in rice seedlings simpler and greatly improving experimental efficiency. Attached Figure Description

[0030] Figure 1 This is a schematic diagram of the overall structure of the culture tank of the present invention;

[0031] Figure 2 This is a schematic cross-sectional view of the culture tank structure of the present invention;

[0032] Figure 3 This is a schematic diagram of the overall structure of the detection cell of the present invention;

[0033] Figure 4 This is a schematic cross-sectional view of the detection cell of the present invention;

[0034] Figure 5 This is a schematic top-view cross-sectional view of the detection pool of the present invention;

[0035] Figure 6 For the present invention Figure 5 A magnified view of the structure at point A in the middle.

[0036] In the diagram: 1. Culture tank; 101. Partition; 102. Water-proof ring; 2. Culture plate; 201. Culture port; 202. Culture net; 203. Water inlet; 204. Drain; 3. Partition plate; 301. Fixing buckle; 302. Isolation cylinder; 303. Isolation ring; 4. Detection tank; 401. Side ear support; 402. One-way toothed groove; 5. Isolation plate; 501. Isolation frame; 502. Sealing ring; 503. Cuvette slot; 504. Rotary tooth; 6. Adjusting tooth; 601. Rotating shaft; 602. One-way knob. Detailed Implementation

[0037] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0038] Please see Figure 1-2A convenient device for monitoring the salt tolerance root vigor of rice seedlings and its usage method are disclosed. The device includes a culture tank 1, a partition 101 fixedly connected to the middle of the culture tank 1, a water-proof ring 102 fixedly connected to the top of the partition 101, a culture plate 2 movably placed above the water-proof ring 102, five sets of cultivation openings 201 on both sides of the culture plate 2, and a cultivation net 202 fixedly installed in the middle of each cultivation opening 201. Conventional culture solution and culture solution with a test-determined salinity are placed on both sides of the partition 101 in the culture tank 1 until the liquid surface contacts the cultivation net 202. Five different sets of rice seeds to be tested are placed in the five sets of cultivation openings 201 for cultivation. A separating auxiliary plate 3 is movably connected to the bottom of the culture plate 2, and the separating auxiliary plate 3 is positioned between the culture plate 2 and the partition 101. In the middle of the water ring 102, the two sides of the partition plate 3 are movably connected with fixing buckles 301. The two sides of the culture plate 2 are provided with slots that match the fixing buckles 301. The partition plate 3 is fixedly connected with an isolation cylinder 302 at the corresponding position at the bottom of each group of culture ports 201. The isolation cylinder 302 is vertically connected. The top of the isolation cylinder 302 is fixedly connected with an isolation ring 303. The top of the isolation ring 303 is fixedly connected with an isolation ring 303. Rotating the fixing buckles 301 makes the culture plate 2 and the partition plate 3 fit tightly together. At this time, the top surface of the isolation ring 303 is pressed tightly against the culture plate 2, thereby preventing the roots of the rice seeds grown from the culture ports 201 from getting tangled between different groups and affecting the accuracy of subsequent detection and comparison.

[0039] Please see Figure 3 The test pool 4 includes a partition in the middle of the test pool 4. The top surface of the test pool 4 and the partition wall is provided with a transverse slide rail groove. The bottom surface of the partition plate 3 is provided with a slide rail, so that the partition plate 3 can slide left and right on the test pool 4, thereby making it easier to rinse the roots growing at the top in the liquid.

[0040] Please see Figure 4-6The top of the front and back of the detection pool 4 is fixedly connected to a side ear bracket 401. A one-way toothed groove 402 is fixedly connected to the middle of the side ear bracket 401. A rotating shaft 601 is movably sleeved in the middle of the one-way toothed groove 402. A one-way knob 602 is fixedly connected to the front of the rotating shaft 601. An adjusting tooth 6 is fixedly connected to the back of the rotating shaft 601. An isolation plate 5 is placed inside the detection pool 4. A sealing ring 502 is fixedly installed on the top surface of the isolation plate 5 corresponding to the position of the isolation cylinder 302. A cuvette slot 503 is fixedly connected inside the sealing ring 502 and the isolation plate 5. The size of the cuvette slot 503 is consistent with the size of a standard cuvette. The cuvette slot 503 can conveniently snap the cuvette into the fixed position on the isolation plate 5. An isolation frame 501 is fixedly connected to the front and back of the isolation plate 5. A helical tooth 504 is provided in the middle of 01. The adjusting tooth 6 meshes with the helical tooth 504. The rotating shaft 601 is provided with a tooth that matches the one-way tooth groove 402. Rotating the rotating shaft 601 causes the adjusting tooth 6 to rotate and drive the helical tooth 504 to move upward, so that the isolation plate 5 moves upward as a whole until the bottom surface of the isolation frame 501 and the isolation cylinder 302 are pressed together. At this time, each group of isolation cylinders 302 forms an independent closed chamber, which facilitates the independent detection of the root system in each group of isolation cylinders 302. After the detection is completed, the one-way knob 602 is pulled outward, so that the tooth on the surface of the rotating shaft 601 disengages from the one-way tooth groove 402. The width of the adjusting tooth 6 is greater than the width of the tooth on the rotating shaft 601. At this time, the tooth on the rotating shaft 601 disengages from the one-way tooth groove 402, and the one-way knob 602 can be reversed smoothly so that the isolation plate 5 falls back to its normal position.

[0041] A convenient method for using a rice seedling salt-tolerant root activity monitoring device includes the following operating steps:

[0042] S1. Place conventional culture medium and culture medium with experimentally set salinity on both sides of the partition 101 in the culture tank 1, until the liquid surface contacts the culture net 202.

[0043] S2. After attaching the partition plate 3 to the bottom surface of the culture plate 2 and securing it with the fastener 301, place it in the culture tank 1 in the center of the whole.

[0044] S3. Place the five different rice plants on the five culture nets 202 respectively, and wait for them to take root;

[0045] S4. During testing, the culture plate 2 and the partition plate 3 are removed from the culture tank 1 as a whole and placed into the test tank 4, so that the slide rail on the bottom surface of the partition plate 3 is engaged with the slide rail groove on the top surface of the test tank 4.

[0046] S5. Pour methylene blue solution into water tank 203 until the solution falls into test pool 4 and completely submerges the rice roots, then let it stand for 2 minutes.

[0047] S6. Open the drain outlet at the bottom of detection pool 4 to drain the internal methylene blue solution, then close the drain outlet.

[0048] S7. Pour clean water into the water tank 203 until the water completely submerges the rice roots.

[0049] S8. Shake the culture plate 2 and the partition plate 3 left and right along the slide rail so that the rice roots are fully rinsed in the clean water, and open the drain of the test pool 4 to discharge the water.

[0050] S9. Repeat steps S7-S8 four to five times;

[0051] S10. After removing the culture plate 2 and the separator plate 3, attach the cuvette to the cuvette slot 503 and place the culture plate 2 and the separator plate 3 back into the center of the detection cell 4.

[0052] S11. Push the one-way knob 602 inward until the teeth on the rotating shaft 601 engage with the one-way tooth groove 402. Rotate the rotating shaft 601 to pull the isolation frame 501 and the isolation plate 5 upward until the sealing ring 502 presses against the bottom surface of the isolation cylinder 302.

[0053] S12. Pour calcium chloride solution into water tank 203 until it completely submerges the rice roots, and let it stand for 5-10 minutes.

[0054] S13. Open the drain outlet of the test pool 4, pull the one-way knob 602 outward to disengage the teeth on the rotating shaft 601 from the one-way tooth groove 402, and then slowly reverse the one-way knob 602 to make the isolation plate 5 fall back.

[0055] S14. After the excess solution flows out of the drain, remove the cuvettes from the cuvette slot 503 in sequence and place them in the spectrophotometer for measurement and recording.

[0056] S15. Place the culture plate 2 and the partition plate 3 containing the tested rice seedlings back into the culture tank 1 for continued cultivation.

[0057] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0058] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A convenient device for monitoring the salt-tolerant root vigor of rice seedlings, comprising a culture tank (1) and a detection tank (4), characterized in that: A partition (101) is fixedly connected to the middle of the culture tank (1). A water-proof ring (102) is fixedly connected to the top of the partition (101). A culture plate (2) is movably placed above the water-proof ring (102). Five sets of culture ports (201) are opened on both sides of the culture plate (2). A culture net (202) is fixedly installed in the middle of the culture port (201). A partition plate (3) is movably connected to the bottom of the culture plate (2). The partition plate (3) is placed between the culture plate (2) and the water-proof ring (102). Fixed buckles (301) are movably connected to both sides of the partition plate (3). The culture plate (2) has slots that match the fixed buckles (301) on both sides. An isolation cylinder (302) is fixedly connected to the corresponding position at the bottom of each set of culture ports (201) on the partition plate (3). The top of the isolation cylinder (302) is fixedly connected to the partition plate (3). An isolation ring (303) is fixedly connected to the top of the front and back of the detection pool (4). A side ear bracket (401) is fixedly connected to the top of the side ear bracket (401). A one-way toothed groove (402) is fixedly connected to the middle of the side ear bracket (401). A rotating shaft (601) is movably sleeved in the middle of the one-way toothed groove (402). A one-way knob (602) is fixedly connected to the front of the rotating shaft (601). An adjusting tooth (6) is fixedly connected to the back of the rotating shaft (601). An isolation plate (5) is placed inside the detection pool (4). A sealing ring (502) is fixedly installed on the top of the isolation plate (5) corresponding to the position of the isolation cylinder (302). A cuvette slot (503) is fixedly connected inside the sealing ring (502) and the isolation plate (5). An isolation frame (501) is fixedly connected to the front and back of the isolation plate (5). A rotating tooth (504) is opened in the middle of the isolation frame (501). The isolation cylinder (302) is open from top to bottom, and the bottom of the detection pool (4) is provided with a drain outlet; The detection pool (4) is provided with a partition in the middle, the top surface of the detection pool (4) and the middle partition wall is provided with a transverse slide rail groove, and the bottom surface of the partition auxiliary plate (3) is provided with a slide rail. The adjusting tooth (6) meshes with the helical tooth (504), and the helical shaft (601) is provided with a meshing tooth that matches the one-way tooth groove (402).

2. The convenient rice seedling salt-tolerant root activity monitoring device according to claim 1, characterized in that: The width of the adjusting tooth (6) is greater than the width of the meshing tooth on the rotating shaft (601).

3. The method of using the convenient rice seedling salt-tolerant root activity monitoring device according to claim 1, characterized in that, The following steps are included: S1. Place conventional culture medium and culture medium with experimentally set salinity on both sides of the partition (101) in the culture tank (1) until the liquid surface contacts the culture net (202); S2. After attaching the partition plate (3) to the bottom surface of the culture plate (2) and securing it with the fastener (301), place the culture plate (2) and the partition plate (3) together in the culture tank (1) in a centered position. S3. Place five different groups of rice seeds on five groups of culture nets (202) and wait for them to take root; S4. During testing, the culture plate (2) and the partition plate (3) are taken out of the culture tank (1) as a whole and placed into the test tank (4) so ​​that the slide rail on the bottom surface of the partition plate (3) is engaged with the slide rail groove on the top surface of the test tank (4). S5. Pour methylene blue solution into the water tank (203) until the solution falls into the test pool (4) and completely submerges the rice roots, and let it stand for 2 minutes. S6. Open the drain outlet at the bottom of the detection pool (4) to drain the internal methylene blue solution, and then close the drain outlet. S7. Pour clean water into the water tank (203) until the water completely submerges the rice roots; S8. Shake the culture plate (2) and the partition plate (3) left and right along the slide rail so that the rice roots are fully rinsed in the clean water. Open the drain of the test pool (4) to drain the water. S9. Repeat steps S7-S8 four to five times; S10. After removing the culture plate (2) and the separator plate (3), attach the cuvette to the cuvette slot (503) and place the culture plate (2) and the separator plate (3) back into the center of the detection cell (4). S11. Push the one-way knob (602) inward until the teeth on the rotating shaft (601) mesh with the one-way tooth groove (402). Rotate the rotating shaft (601) to pull the isolation frame (501) and the isolation plate (5) upward until the sealing ring (502) presses against the bottom surface of the isolation cylinder (302). S12. Pour calcium chloride solution into the water tank (203) until it completely submerges the rice roots, and let it stand for 5-10 minutes. S13. Open the drain of the test pool (4), pull the one-way knob (602) outward to disengage the teeth on the rotating shaft (601) from the one-way groove (402), and slowly reverse the one-way knob (602) to make the isolation plate (5) fall back. S14. After the excess solution flows out of the drain, remove the cuvettes from the cuvette slot (503) in sequence and place them in the spectrophotometer for measurement and recording. S15. Place the culture plate (2) and the partition plate (3) containing the tested rice seedlings back into the culture tank (1) for continued cultivation.

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