A breeding method for a new variety of Babylonia crassissima

Abstract

The present invention provides a breeding method for a new variety of Babylonia crassissima. The method comprises the following steps: selecting thick-shelled individuals of Babylonia crassissima as a breeding population, carrying out cross-breeding, and harvesting the egg capsules of Babylonia crassissima; incubating the egg capsules and culturing the larvae to obtain juvenile snails; feeding the juvenile snails with a thickening agent to obtain the first generation; selecting the first generation based on the shell thickness, arranging them in descending order of shell thickness, intercepting and retaining the superior ones from the first generation, and screening out thick-shelled individuals as an expansion and propagation population; carrying out large-scale breeding on the expansion and propagation population, repeating continuously for more than 2 generations to obtain Babylonia crassissima with stable inheritance of thick shells. The thick-shelled Babylonia crassissima cultivated by the present invention has the advantages of thick shells and strong compressive resistance, and there are significant improvements in the shell thickness, shell weight and body weight of the cultivated thick-shelled Babylonia crassissima.

Description

[0001] This application is a divisional application of the invention patent application with the application date of May 30, 2024, the application number of 2024106881938, and the invention title of "A Breeding Method for a New Variety of Babylonia crassa". Technical Field

[0002] The present invention belongs to the technical field of genetic breeding of Babylonia, and more specifically, relates to a breeding method for a new variety of Babylonia crassa. Background Art

[0003] Babylonia, also known as sea pig snail, sweet snail, south wind snail, yellow snail, and flower snail, belongs to Mollusca, Gastropoda, Prosobranchia, Neogastropoda, Buccinidae, and Babylonia.

[0004] Currently, there have been many studies on germplasm improvement of existing Babylonia, such as "Haitai No. 1" of Babylonia areolata (variety registration number GS-01-008-2018), "Haiyifeng 11" of bay scallop (variety registration number GS-01-012-2022), "Yongle No. 1" of Sinonovacula constricta (variety registration number GS-01-004-2020), "Haidai No. 4" of Crassostrea gigas (variety registration number GS-02-008-2022), etc. However, these newly bred varieties are not selected for shell thickness. In addition, especially for Babylonia with a naturally thin and brittle shell, during the production and cultivation of Babylonia, different degrees of damage to the shell of Babylonia often occur during fishing, pond changing, transportation and other links, resulting in casualties. Therefore, how to increase the shell thickness of Babylonia, thereby resisting seawater acidification, increasing the survival rate of Babylonia, and reducing losses has become a technical problem that needs to be solved urgently. Summary of the Invention

[0005] Aiming at the above-mentioned existing technical problems, the primary object of the present invention is to provide a breeding method for a new variety of Babylonia crassa. The Babylonia crassa bred by the breeding method has the advantages of thick shell and strong compressive resistance, and the shell thickness, shell weight and body weight of the bred Babylonia crassa are all significantly improved.

[0006] In order to achieve the above object, the present invention is realized through the following technical solutions:

[0007] The present invention claims to protect a breeding method for a new variety of Babylonia crassa, including the following steps:

[0008] S1. Select Babylonia individuals with thick shells as the breeding population, mix the breeding population, and harvest the egg capsules of Babylonia.

[0009] S2. The Babylonia areolata egg capsules are hatched and the larvae are cultivated to obtain juvenile snails; the juvenile snails are fed with a thickening agent to obtain the first generation; subsequently, the first generation is selected and bred based on the shell thickness. They are arranged in descending order of shell thickness, and the first generation is intercepted and the superior ones are retained, and the thick-shelled individuals are selected as the propagation population;

[0010] S3. The propagation population is subjected to large-scale breeding according to the hybridization in step S1 and the operations in step S2, and is continuously repeated for more than 2 generations to obtain the thick-shelled Babylonia areolata with stable inheritance;

[0011] The thickening agent includes a growth promoter, a compound vitamin and a compound multi-mineral; the growth promoter is extracted and prepared from the following raw materials; by weight, the raw materials include: 4-5 parts of Cyanotis arachnoidea C. B. Clarke, 2-3 parts of Gentiana scabra Bunge, 1-2 parts of Alisma orientale (Sam.) Juz. and 1-2 parts of Prunella vulgaris L.

[0012] In the present invention, the thick-shelled individuals of Babylonia areolata are selected as the breeding population, and the breeding population is hybridized. Subsequently, the Babylonia areolata are cultured with a thickening agent to obtain the first generation. The first generation is selected and bred based on the shell thickness, and the superior ones are retained with a selection pressure of 10%, and the propagation population is selected. The propagation population is subjected to large-scale breeding for more than 2 generations, and the thick-shelled Babylonia areolata with stable inheritance is obtained.

[0013] In the present invention, the thickening agent includes a growth promoter, a compound vitamin and a compound multi-mineral. The growth promoter contains various nutrient elements such as trace elements, steroid compounds, polysaccharides, vitamins, amino acids, etc., which can be absorbed by aquatic animals and play a role in promoting growth in the Babylonia areolata, promoting the absorption of calcium and magnesium, improving the growth rate, enhancing the disease resistance of the Babylonia areolata, promoting metabolism and protein synthesis, thereby promoting the increase in the shell thickness of the Babylonia areolata.

[0014] In the present invention, the compound multi-mineral can maintain the survival and body fluid balance of the Babylonia areolata, promote the growth of the Babylonia areolata, increase the thickness of the shell layer of the Babylonia areolata, supplement various nutritional requirements of the Babylonia areolata, and further promote the better accumulation of the shell layer of the Babylonia areolata. In the present invention, the compound vitamin can promote the growth of the Babylonia areolata, improve the feeding of the Babylonia areolata, enhance the metabolism, enzyme activity and cell viability of the Babylonia areolata, promote the digestion, absorption and metabolism of the components in the bait, and further convert them into the nutritional components required by the shell layer of the Babylonia areolata; it can supplement nutrition and improve the health level of the Babylonia areolata body.

[0015] Through the above breeding method and the combination with a specific thickening agent, the thick-shelled Babylonia areolata cultivated in the present invention has the advantages of thick shell and strong compressive resistance, and the shell thickness, shell weight and body weight of the cultivated thick-shelled Babylonia areolata are all significantly improved.

[0016] Preferably, in step S1, the thick-shelled individuals of Babylonia areolata with the top 10-20% thickness are selected as the breeding population.

[0017] Preferably, in the step S1, the male-female ratio of the hybridized Babylonia areolata is 1:1 to 3.

[0018] Preferably, in the step S2, the first filial generation is selected and the excellent ones are retained with a selection pressure of 10-20%.

[0019] Further preferably, by weight, the compound vitamin includes: 1-2 parts of vitamin A acetate, 2-3 parts of DL-α-tocopheryl acetate, vitamin B 1 1-2 parts, vitamin B 2 1-2 parts, vitamin B 6 2-3 parts, vitamin B 12 1-2 parts, 1-2 parts of vitamin C, 1-2 parts of nicotinamide, 1-2 parts of D-calcium pantothenate, 3-4 parts of folic acid.

[0020] More preferably, by weight, the compound vitamin includes: 1 part of vitamin A acetate, 2 parts of DL-α-tocopheryl acetate, vitamin B 1 1 part, vitamin B 2 1 part, vitamin B 6 2 parts, vitamin B 12 1 part, 1 part of vitamin C, 1 part of nicotinamide, 1 part of D-calcium pantothenate, 3 parts of folic acid.

[0021] Further preferably, by weight, the compound multimineral includes: 10-15 parts of calcium citrate, 1-2 parts of manganese amino acid, 1-2 parts of magnesium sulfate, 1-2 parts of potassium chloride, 1-2 parts of iron glycinate, 1-2 parts of copper glycinate, 1-2 parts of zinc amino acid, 1-2 parts of cobalt chloride, 1-2 parts of potassium iodate, 1-2 parts of sodium selenite, 1-2 parts of lysine, 1-2 parts of methionine.

[0022] More preferably, by weight, the compound multimineral includes: 10 parts of calcium citrate, 1 part of manganese amino acid, 1 part of magnesium sulfate, 1 part of potassium chloride, 1 part of iron glycinate, 1 part of copper glycinate, 1 part of zinc amino acid, 1 part of cobalt chloride, 1 part of potassium iodate, 1 part of sodium selenite, 1 part of lysine, 1 part of methionine.

[0023] Preferably, the mass ratio of the growth promoter, the compound vitamin and the compound multimineral is: 3-5:1-2:1-2.

[0024] Preferably, in the step S1, before hybridization, the selected population is fed with one or more of nereis, oyster or crab. Feeding nereis, oyster or crab before hybridization can strengthen the nutrition of Babylonia areolata and is beneficial to its growth and development. More specifically, it is fed every evening, and the feeding amount of oyster or nereis accounts for 5-10% of the weight of Babylonia areolata, and the feeding amount of crab accounts for 20-40% of the weight of Babylonia areolata.

[0025] Preferably, in the step S2, the thickener-mixed bait is fed to conduct intermediate culture of juvenile clams and grow-up clam culture to obtain the first-generation offspring. Further preferably, the addition amount of the thickener in the bait is 1-5 wt%. More preferably, the addition amount of the thickener in the bait is 2-4 wt%.

[0026] Further preferably, the feeding amount of the bait accounts for 5-12% of the weight of the conch. More specifically, in the early stage, it is fed for 2 days and then stopped for 1 day, and the feeding amount accounts for about 5-7% of the weight of the Babylonia areolata; in the middle and late stages, it is fed once every 2 days, and the feeding amount accounts for about 10-12% of the weight of the Babylonia areolata.

[0027] Preferably, the raw materials of the growth promoter are mixed with petroleum ether, extracted, and post-treated to obtain the growth promoter.

[0028] Further preferably, the raw materials can be crushed before mixing, and the particle size range of the crushed raw materials can be 120-300 mesh.

[0029] Further preferably, the ratio of the raw materials to petroleum ether is 1:6-10 g / mL.

[0030] Further preferably, the number of extraction times is 2-3 times, and the total extraction time is 5-6 h.

[0031] Further preferably, the extraction temperature is 60-90 °C.

[0032] Further preferably, the post-treatment is: filtering the extract, and concentrating the filtrate under reduced pressure to remove the solvent.

[0033] Preferably, during the breeding process, the pH value of the seawater is controlled to be 7.8-8.6, the salinity is 28-34, the temperature is 24-30 °C, and the ventilation rate is maintained at 0.008-0.02 V / V·min.

[0034] Specifically, the thick-shell individuals of Babylonia areolata can be selected by the conventional methods in the art, such as using a digital display thickness gauge or a laser thickness gauge, etc.

[0035] Preferably, in the step S2, different single-celled algae can be fed at different planktonic periods during the larval cultivation stage of Babylonia areolata. For example, the bait fed to the early-stage veliger larvae can be Chrysochromulina, the bait fed to the middle-stage veliger larvae can be Chrysochromulina and / or Platymonas, and the bait fed to the late-stage veliger larvae can be one or more of Thalassiosira, Chaetoceros or Platymonas. More specifically, during the intermediate culture of juvenile clams, the bait fed can be one or more of Artemia larvae and oysters; more specifically, 0.1-1 wt% of taurine and 0.1-1 wt% of EM bacteria can be added to the oysters.

[0036] Compared with the prior art, the present invention has the following beneficial effects:

[0037] Through the above breeding method and in combination with a specific thickening agent, the cultivated Babylonia areolata with thick shells has the advantages of thick shells and strong compressive resistance. The shell thickness, shell weight and body weight of the cultivated Babylonia areolata with thick shells have all increased significantly, greatly improving the ability to withstand fishing and transportation, and having good market application prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] Figure 1 It is a breeding roadmap for the new variety of Babylonia areolata with thick shells.

[0039] Figure 2 It is a schematic diagram of the measurement position A of the shell thickness of Babylonia areolata.

[0040] Figure 3 It is a schematic diagram of the edges of the shells of Babylonia areolata before and after breeding. Among them, Figure 3 At B in is a schematic diagram of the smooth and intact edge of the individual shell of the cultivated Babylonia areolata with thick shells; Figure 3 At C in is a schematic diagram of the shell breakage of the Babylonia areolata before breeding (thin-shelled individuals regardless of size). DETAILED DESCRIPTION OF THE EMBODIMENTS

[0041] The present invention will be further described below in conjunction with the accompanying drawings of the specification and specific embodiments, but the embodiments do not limit the present invention in any form. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the technical field.

[0042] The phrase "early veliger larva" refers to the developmental stage in which two residual yolk masses are clearly visible in the body of the Babylonia areolata larva, and there are two veligers that are oval, symmetrically arranged left and right, and the edges are densely covered with cilia.

[0043] The phrase "mid-veliger larva" refers to the stage in which the yolk mass of the Babylonia areolata larva disappears and obvious characteristics such as eyes, forefeet, primary shell, and a pair of antennae appear.

[0044] The phrase "late veliger larva" refers to the stage in which the head of the Babylonia areolata larva has a proboscis, a siphon, obvious differentiation of gill filaments, and 1-2 rows of reddish-brown pigment spots appear on the spiral layer.

[0045] The phrase "juvenile snail" refers to the stage in which the veliger of the Babylonia areolata larva atrophies, the cilia fall off, and it changes from floating to crawling.

[0046] The phrase "acidity and alkalinity" refers to the degree of acidity and alkalinity of a solution, generally expressed by the pH value.

[0047] Example 1 A breeding method for Babylonia areolata with thick shells

[0048] As Figure 1 shown, a breeding method for a new variety of Babylonia areolata with thick shells includes the following steps:

[0049] (1) Selection of broodstock: The geographical population of Babylonia areolata from Hainan is selected as the basic population with advantages for broodstock. The individuals with thick shells in the top 10% of thickness are selected from them using a laser thickness gauge as the selected breeding population. The male and female are distinguished according to gender characteristics and paired according to the male-female ratio of 1:2.

[0050] (2) Accelerated maturation and mating of broodstock: The nutritional enhancement of the selected breeding population is carried out, feeding once every evening at 5 o'clock, and taking turns to feed oysters, nereids, and crabs every day. During this period, the water temperature is controlled at 25 - 30 °C, the salinity is 28 - 34, and the pH value is 7.8 - 8.6. After the broodstock is sexually mature, they are mixed for mating, fertilized, and spawned, and the egg sacs (egg capsules) of Babylonia areolata that cluster and attach to the bottom of the pool or sand are collected.

[0051] (3) Selection and breeding of the first generation

[0052] Hatching, larval cultivation, intermediate cultivation of juvenile snails, and adult snail farming: The egg sacs of Babylonia areolata are placed in an incubation frame and then placed in a nursery pond. The water temperature in the nursery pond is controlled at 27 - 28 °C, the salinity is 28 - 30, and the pH value is 7.8 - 8.2. After 7 days, the fertilized eggs complete embryonic development in the egg sacs, and then the larvae of Babylonia areolata hatch from the top of the egg sacs. The density of the larvae of Babylonia areolata is controlled at 0.5 - 1.0 individuals / mL, with slight aeration and no water change in a closed system. Feed the bait at fixed points twice a day, and the feeding times are 8:00 in the morning and 20:00 in the evening. The early veliger larvae are fed with chrysophyta, the middle veliger larvae are fed with chrysophyta and platymonas, and the late veliger larvae are fed with thalassiosira, chaetoceros, and platymonas. After 12 days of cultivation, the larvae of Babylonia areolata successfully settle to the bottom and attach and metamorphose into crawling juvenile snails. The bait for the juvenile snails is changed to feed artemia larvae, and after 7 days, it is changed to feed crushed oyster meat. 0.5 wt% taurine and 0.5 wt% EM bacteria are added to the oysters, and after soaking for 15 minutes, it is evenly spread throughout the pool.

[0053] After 30 days of intermediate cultivation, the juvenile snails become young snails. The sand and young snails of Babylonia areolata in the intermediate cultivation pond are shoveled into a stainless - steel snail sieve with a shovel, and snails of different specifications are separated for pond farming.

[0054] The young snails of Babylonia areolata are put into the farming pond. During the farming period, there is running water for 24 hours, and blue - striped scad is fed throughout the process. The feeding amount of blue - striped scad accounts for 5 - 12% of the weight of the snails. When feeding, small fish are soaked with a thickening agent accounting for 3.0% of the weight of the blue - striped scad before feeding, and it is appropriate that there is no remaining bait within 20 minutes after feeding. In the early stage, feed for 2 days and stop for 1 day, and the feeding amount accounts for about 5 - 7% of the weight of Babylonia areolata; in the middle and late stages, feed once every 2 days, and the feeding amount accounts for about 10 - 12% of the weight of Babylonia areolata. After 8 months of farming, the finished snails (the first generation) are harvested.

[0055] Among them, the thickening agent includes a growth promoter, a compound vitamin, and a compound multi - mineral, and the mass ratio of the three is 4:1:1 in sequence.

[0056] Among them, the growth promoter is extracted from the following raw materials. By weight, the raw materials include: 4 parts of Cyanotis arachnoidea C. B. Clarke, 2 parts of Gentiana scabra Bunge, 1 part of Alisma orientale (Sam.) Juzep. and 1 part of Prunella vulgaris L.

[0057] The extraction method of the growth promoter is as follows: The above raw materials are crushed into 200 meshes, the ratio of the raw materials to petroleum ether is 1:8 g / mL, extraction is carried out at 70 °C, the number of extraction times is 2 times, and the total extraction time is 5.5 h; the extract is filtered, and the filtrate is concentrated under reduced pressure to remove the solvent, thus obtaining the growth promoter.

[0058] By weight, the compound vitamin includes: 1 part of vitamin A acetate, 2 parts of DL-α-tocopheryl acetate, 1 part of vitamin B 1 1, 1 part of vitamin B 2 1, 1 part of vitamin B 6 2, 1 part of vitamin B 12 1, 1 part of vitamin C, 1 part of nicotinamide, 1 part of D-calcium pantothenate, 3 parts of folic acid.

[0059] By weight, the compound multi-minerals include: 10 parts of calcium citrate, 1 part of manganese amino acid complex, 1 part of magnesium sulfate, 1 part of potassium chloride, 1 part of iron glycinate, 1 part of copper glycinate, 1 part of zinc amino acid complex, 1 part of cobalt chloride, 1 part of potassium iodate, 1 part of sodium selenite, 1 part of lysine, 1 part of methionine.

[0060] Select the finished snails (the first generation) with the shell thickness as the target trait. Arrange them in descending order of shell thickness, and intercept and retain the superior offspring of the inbred mating with a selection pressure of 10%, and select the thick-shell individuals as the propagation population.

[0061] (4) Large-scale breeding: Then, continue to carry out large-scale breeding on the propagation population according to steps (2) and (3). After continuous purification for 3 generations, a new variety of Babylonia areolata with stable inheritance of thick shells is obtained.

[0062] Example 2 A breeding method for Babylonia areolata with thick shells

[0063] The difference between this example and Example 1 is that:

[0064] By weight, the raw materials include: 5 parts of Cyanotis arachnoidea C. B. Clarke, 2 parts of Gentiana scabra Bunge, 2 parts of Alisma orientale (Sam.) Juzep. and 2 parts of Prunella vulgaris L.

[0065] Example 3 A breeding method for Babylonia areolata with thick shells

[0066] The difference between this example and Example 1 is that:

[0067] By weight, the raw materials include: 4 parts of Cyanotis arachnoidea C. B. Clarke, 3 parts of Gentiana scabra Bunge, 1 part of Alisma orientale (Sam.) Juzep. and 2 parts of Prunella vulgaris L.

[0068] Control Example 1

[0069] The difference between this comparative example and Example 1 is that no thickener is used during the breeding process.

[0070] Comparative Example 2

[0071] The difference between this comparative example and Example 1 is that the thickener only includes compound vitamins and compound multi-minerals.

[0072] Comparative Example 3

[0073] The difference between this comparative example and Example 1 is that the thickener only includes growth promoters and compound vitamins.

[0074] Comparative Example 4

[0075] The difference between this comparative example and Example 1 is that the thickener only includes growth promoters and compound multi-minerals.

[0076] Test Example 1

[0077] The above examples and comparative examples were tested using the following method:

[0078] Shell thickness: Randomly select 30 Babylonia areolata, and measure the shell thickness of each Babylonia areolata with a digital vernier caliper. The measurement position of the Babylonia areolata is at the body whorl near the aperture of the Babylonia areolata (as Figure 2 shown), record and calculate the average shell thickness.

[0079] Shell weight: Randomly select 30 Babylonia areolata, remove the soft body part, keep the shell, and weigh it with an electronic balance (500g / 0.01g), record and calculate the average shell thickness.

[0080] Body weight: Randomly select 30 Babylonia areolata, remove the shell, keep the soft body part, and weigh it with an electronic balance (500g / 0.01g), record and calculate the average shell thickness.

[0081] Calculation formula for test data:

[0082] Average shell thickness = total shell thickness of 30 Babylonia areolata ÷ 30;

[0083] Average shell weight = total shell weight of 30 Babylonia areolata ÷ 30;

[0084] Average body weight = total weight of 30 Babylonia areolata ÷ 30;

[0085] The comparison of the test data of the examples and comparative examples is shown in Table 1 below.

[0086] Table 1

[0087]

[0088] As can be seen from the examples, the Babylonia areolata obtained by breeding the selected line of Babylonia areolata with a thick shell obtained by a method for breeding a new variety of Babylonia areolata provided by the present invention, such as Figure 3 shown, the shell thickness, shell weight and body weight of the cultivated Babylonia areolata with a thick shell are significantly improved, and the edges of the individual shells of the Babylonia areolata with a thick shell are smooth and without damage, while the shells of the Babylonia areolata before breeding are damaged. In the same breeding time, the average shell thickness of the Babylonia areolata with a thick shell cultivated by the present invention is 0.80 - 0.83 mm, the average shell weight is 6.63 - 6.82 g, and the average body weight is 13.47 - 13.66 g.

[0089] As can be seen from Example 1 and Comparative Example 1, the shell thickness, body weight and shell weight of the Babylonia areolata cultivated without using a thickening agent are significantly worse than those of the Babylonia areolata with a thick shell cultivated by the present invention.

[0090] As can be seen from Example 1, Comparative Example 1 and Comparative Examples 2 - 4, when breeding with the selected line of Babylonia areolata with a thick shell obtained by a method for breeding a new variety of Babylonia areolata provided by the present invention, in the same breeding time, the growth promoter, compound vitamin and compound multi - mineral play a synergistic role. The three growth indexes of the shell thickness, shell weight and body weight of the Babylonia areolata with a thick shell selected by the present invention are significantly increased compared with the original Babylonia areolata, and the economic traits are significantly improved.

[0091] The foregoing examples are illustrative only and are used to explain some of the features of the method of the present invention. The appended claims are intended to claim as broad a scope as can be conceived, and the examples presented herein are demonstrated by the applicant's actual test results. Therefore, the applicant's intention is that the appended claims should not be limited by the selection of examples that illustrate the features of the present invention. Some of the numerical ranges used in the claims also include sub - ranges within them, and variations within these ranges should also be interpreted as being covered by the appended claims where possible.

Claims

1. Application of a thickening agent in the breeding of thick-shelled babylonia, characterized in that: The invention comprises a growth promoter, a compound vitamin and a compound multi-mineral; the growth promoter is prepared by extracting the following raw materials; the raw materials include, by weight: 4-5 parts of dew grass, 2-3 parts of gentian, 1-2 parts of oriental rhizome and 1-2 parts of selfheal; The compound vitamins include, by weight: 1-2 parts of vitamin A acetate, 2-3 parts of DL-α-tocopherol acetate, 1-2 parts of vitamin B1, 1-2 parts of vitamin B2, 2-3 parts of vitamin B6, and 1-2 parts of vitamin B6. 12 1-2 parts, vitamin C 1-2 parts, nicotinamide 1-2 parts, D-calcium pantothenate 1-2 parts, folic acid 3-4 parts; the raw material of the growth promoter is mixed with petroleum ether, extracted, and post-treated to obtain the growth promoter; the ratio of the raw material to petroleum ether is 1:6-10g / mL; the extraction temperature is 60-90°C; The composite multi-mineral comprises: 10-15 parts of calcium citrate, 1-2 parts of amino acid manganese, 1-2 parts of magnesium sulfate, 1-2 parts of potassium chloride, 1-2 parts of glycine iron, 1-2 parts of glycine copper, 1-2 parts of amino acid zinc, 1-2 parts of cobalt chloride, 1-2 parts of potassium iodate, 1-2 parts of sodium selenite, 1-2 parts of lysine, and 1-2 parts of methionine; The mass ratio of the growth promoter, the complex vitamin and the complex multi-mineral is 3-5:1-2:1-2.

2. The application according to claim 1, characterized in that: The extraction is performed 2 to 3 times, and the total extraction time is 5 to 6 hours.

3. The use according to claim 1, characterized in that: The post-treatment is as follows: filtering the extract, and concentrating the filtrate under reduced pressure to remove the solvent.

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