Method for mixed feeding of two kinds of aphid parasitic wasps
By raising cocoon aphid and cotton aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid aphid a
Patent Information
- Application Number
- CN202411616068.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-13
- Publication Date
- 2025-02-14
- Estimated Expiration
- 2044-11-13
AI Technical Summary
In the prior art, raising aphid parasitic wasps alone can easily lead to a large number of young aphids not being parasitized, resulting in waste of resources and inefficient breeding.
The method of raising two aphid parasitic wasps in a mixed feeding was used to inoculate the tobacco aphid and the cotton aphid aphid aphid aphid wasps, and the control of different time periods and inoculation density was used to avoid interspecies competition and improve parasitic efficiency.
Through mixed feeding, the aphid parasitic rate is improved, manpower and material resources are saved, and the large-scale reproduction efficiency of aphid natural enemy products is improved.
Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of artificial propagation of natural enemy insects and biological control of agricultural pests, and in particular relates to a method for mixed breeding of two aphid parasitic wasps. Background Art
[0002] Aphids can not only harm crops but also spread viruses, greatly affecting the yield of crops. Aphididae tobacco and Aphididae gossypii are parasitic natural enemies of cotton aphids, green peach aphids, and other aphids. In some countries, they have been used to control cotton aphids in greenhouse vegetables. At present, most natural enemy insects are raised separately. Since parasitic wasps prefer to parasitize 2-3 instar aphids, a large number of 1-instar aphids are not parasitized. In addition, aphids are also constantly reproducing offspring. Therefore, inoculating a single natural enemy will easily lead to a large number of young aphids not being parasitized, resulting in a waste of resources and low breeding efficiency. Summary of the invention
[0003] The technical problem to be solved by the invention is to provide a method for mixed breeding of two aphid parasitic wasps with short time and good effect.
[0004] In order to solve the above technical problems, the technical solution adopted by the present invention includes the following steps: The first step is to breed host plants Fill the small nutrient pot with nutrient soil to a height of 4-6 cm, then sow the eggplant seeds for seedling cultivation; when the eggplant seedlings are 4-6 cm tall, transplant them into the medium-sized seedling pot, spray water regularly, and set aside when the eggplant seedlings grow to 6-8 leaves; Step 2 Aphid inoculation and reproduction Inoculate aphids, the aphids are peach aphids, cut the eggplant vine leaves with aphids into squares with a side length of 2 cm, put them on the new eggplant vine plants with the back side facing down, and put 6-8 leaves on each plant; put the single plant in an insect cage with a 120-mesh gauze net, the temperature in the artificial climate is 20-28℃, and the humidity is 50-80%; after the aphids are transferred to the new eggplant vine plants, remove the square old leaves; after 4-5 days, when the number of aphids on the new eggplant vine plants reaches 800-1000 per plant, inoculate with tobacco aphid braconids; Step 3: Obtaining and inoculating two species of aphid parasitic wasps Bee pupae of two parasitic wasps, Aphididae fasciatus and Aphididae scoparia, were collected from eggplant vines, and large and brightly colored individuals were selected and placed in an insect culture tube. The lid of the insect culture tube was provided with a round hole, which was glued with a 100-mesh gauze. The insect culture tube was placed in a constant temperature box with a temperature of 24-26°C and a humidity of 60-80%, and the emergence of the bee pupae was observed every day. Adult Aphididae fasciatus that emerged within 24 hours were placed in an insect cage, and the inoculation density of Aphididae fasciatus was 40-50 heads / plant. Four days after the inoculation of Aphididae fasciatus, adult Aphididae scoparia that emerged within 24 hours were inoculated in the insect cage again, and the inoculation density of Aphididae scoparia was 40-50 heads / plant. After the inoculation, the insect cage was placed in an artificial climate room with a temperature of 25-28°C and a humidity of 50-80%. Step 4: Collection of parasitic wasps When only white or light brown spherical pupae of Aphididae cirrhosae appear on the leaves of the eggplant seedlings in the insect cage, start recording the time, wait for 2-3 days, and collect a large number of black pupae of Aphididae cirrhosae after they appear; pick off the leaves carrying the two types of parasitic wasp pupae, and rinse them with clean water using a pressurized water gun to obtain the two types of parasitic wasp pupae; Step 5: Remove impurities The two types of parasitic wasp pupae washed down are repeatedly rinsed and separated to remove the heavy small soil pieces, sand and other impurities; the two types of parasitic wasp pupae are then placed on a gauze net to dry; after 1-2 days, the two types of parasitic wasp pupae are placed on a 40-mesh sieve to sieve out the fine impurities, and the two types of parasitic wasp pupae will not fall off; Step 6: Separation of two parasitic wasps The pupae of the two parasitic wasps are of different sizes, and the pupae of the tobacco aphid bracon wasp are larger than the pupae of the cotton aphid aphid wasp; the two pupae are separated by placing sieves of different meshes; a 40-mesh sieve is placed on the lower layer to ensure that the pupae that are sieved down do not fall down, 25-mesh and 30-mesh sieves are placed on the middle layer to separate the smaller tobacco aphid bracon wasp and the cotton aphid aphid wasp pupae, and the top layer is a 20-mesh sieve to separate the larger tobacco aphid bracon wasp; sieve from top to bottom in sequence, first put the two pupae on the top layer of the sieve, sieve repeatedly twice, the pupae left on the top layer of the sieve are larger tobacco aphid bracon wasp, and the pupae that fall into the middle layer of the 25-mesh and 30-mesh sieves are smaller tobacco aphid bracon wasp and cotton aphid aphid wasp pupae; then sieve the two specifications of the middle layer sieves from high to low for 2-3 times respectively, the pupae left on the middle layer of the sieve are tobacco aphid bracon wasp pupae, and the pupae that leak into the lower layer of the 40-mesh sieve are cotton aphid aphid wasp pupae; in this way, the pupae of the two parasitic wasps are separated and collected separately for later use.
[0005] Its additional technical features are: the small nutrient pot is a barrel with a diameter of 8 cm and a height of 7.5 cm, the medium seedling pot is a barrel with a diameter of 12 cm and a height of 7.5 cm, the insect cage is 50 cm long, 50 cm wide and 50 cm high, the insect tube is 2.5 cm in diameter and 8 cm high, the diameter of the round hole in the insect tube cover is 1.5 cm, and the rated current of the high-pressure water gun is 2.4m 3 / h, power 0.75kW.
[0006] The method for mixed breeding of two aphid parasitic wasps provided by the present invention comprises the following steps: inoculating two parasitic natural enemies, Aphididae fasciatus and Aphididae spp., at different time periods, respectively; the two parasitic wasps are inoculated 4 days apart, thereby avoiding interspecific competition between the two parasitic wasps and improving the parasitism efficiency of a single bee species. The two aphid parasitic wasps are inoculated at different time periods, thereby avoiding interspecific competition between adult bees. The pupae of the two parasitic wasps are of different sizes, and the two parasitic wasps are separated by sieves of different mesh sizes. In addition, the two parasitic wasps can be collected at the same time, saving manpower and material resources. Since the two parasitic wasps have different development periods from the egg-aphid stage, Aphididae fasciatus is about 2 days longer than Aphididae spp. At 25° C., the egg-aphid stage of Aphididae fasciatus is about 7 days, while the Aphididae spp. is about 5 days, so the two parasitic wasps can be collected at the same time after the pupae of Aphididae spp. are formed in large quantities. The method for mixed breeding of two aphid parasitic wasps provided by the present invention can obtain two aphid natural enemy products at the same time. When applied in the field in the future, different aphid parasitic wasps can be selected according to different climatic conditions and aphid occurrence patterns, thereby greatly improving the aphid control effect.
[0007] The present invention provides a method for mixed breeding of two aphid parasitic wasps. While continuing to use eggplant as a host plant, the method for mixed breeding of two aphid parasitic wasps is invented from the perspective of improving the parasitism rate of aphids and saving labor, thereby further improving the large-scale breeding efficiency of aphid parasitic wasps. In the case where the aphid carrying capacity on the host plant has an upper limit, the aphid parasitism effect is improved by different bee-inoculation times and bee-inoculation types, opening up a new way to improve the large-scale breeding efficiency of aphid natural enemies. According to needs, the small nutrient pot is a barrel-shaped with a diameter of 8 cm and a height of 7.5 cm, the medium-sized seedling pot is a barrel-shaped with a diameter of 12 cm and a height of 7.5 cm, the insect cage is 50 cm long, 50 cm wide and 50 cm high, the insect tube is 2.5 cm in diameter and 8 cm high, and the high-pressure water gun has a rated current of 2.4m 3 / h, power 0.75kW. DETAILED DESCRIPTION
[0008] The method for mixed breeding of two aphid parasitic wasps of the present invention is further described in detail below in conjunction with the embodiments. Example 1
[0009] A method for mixed breeding of two aphid parasitic wasps of the present invention comprises the following steps: The first step is to breed host plants Fill the small nutrient pot with nutrient soil to a height of 4 cm, then sow the eggplant seeds for seedling cultivation; when the eggplant seedlings are 4 cm tall, transplant them into the medium-sized seedling pot, spray water regularly, and set aside when the eggplant seedlings grow to 6 leaves; Step 2 Aphid inoculation and reproduction Aphids were inoculated, and the aphids were green peach aphids. The leaves of the eggplant seedlings with aphids were cut into squares with a side length of 2 cm, and placed with the back side facing downward on the new eggplant seedlings, with 6 leaves placed on each plant; the single plant was placed in an insect cage with a 120-mesh gauze net, and the temperature in the artificial climate was 20°C and the humidity was 50%; after the aphids were transferred to the new eggplant seedlings, the square old leaves were removed; after 4 days, when the number of aphids on the new eggplant seedlings reached 800 per plant, the tobacco aphid braconid was inoculated; Step 3: Obtaining and inoculating two species of aphid parasitic wasps Bee pupae of two parasitic wasps, Aphididae fasciatus and Aphididae scoparia, were collected from eggplant vines, and large and brightly colored individuals were selected and placed in an insect culture tube. The lid of the insect culture tube was provided with a round hole, which was glued with a 100-mesh gauze. The insect culture tube was placed in a constant temperature box with a temperature of 24°C and a humidity of 60%, and the emergence of the bee pupae was observed every day. Adult Aphididae fasciatus that emerged within 24 hours were placed in an insect cage, and the inoculation density of Aphididae fasciatus was 40 per plant. Four days after the inoculation of Aphididae fasciatus, adult Aphididae scoparia that emerged within 24 hours were inoculated in the insect cage again, and the inoculation density of Aphididae scoparia was 40 per plant. After the inoculation, the insect cage was placed in an artificial climate room with a temperature of 25°C and a humidity of 50%. Step 4: Collection of parasitic wasps When only white or light brown spherical pupae of Aphididae cirrhosae appeared on the leaves of the eggplant seedlings in the insect cage, start recording the time, wait for 2 days, and collect a large number of black pupae of Aphididae cirrhosae after they appeared; pick off the leaves carrying the two types of parasitic wasp pupae, and rinse them with clean water using a pressurized water gun to obtain the two types of parasitic wasp pupae; Step 5: Remove impurities The two types of parasitic wasp pupae that were washed down were repeatedly rinsed and separated to remove the heavier small soil pieces, sand and other impurities; the two types of parasitic wasp pupae were then placed on a gauze net to dry; one day later, the two types of parasitic wasp pupae were placed on a 40-mesh sieve to sieve out the fine impurities, and the two types of parasitic wasp pupae would not fall off; Step 6: Separation of two parasitic wasps The pupae of the two parasitic wasps are of different sizes, and the pupae of the tobacco aphid bracon wasp are larger than the pupae of the cotton aphid aphid wasp; the two pupae are separated by placing sieves of different meshes; a 40-mesh sieve is placed on the lower layer to ensure that the pupae that are sieved down do not fall down, 25-mesh and 30-mesh sieves are placed on the middle layer to separate the smaller tobacco aphid bracon wasp and the cotton aphid aphid wasp pupae, and the top layer is a 20-mesh sieve to separate the larger tobacco aphid bracon wasp; sieving from top to bottom, first put the two pupae on the top layer of the sieve, sieve twice repeatedly, the pupae left on the top layer of the sieve are larger tobacco aphid bracon wasp, and the pupae that fall into the middle layer of the 25-mesh and 30-mesh sieves are smaller tobacco aphid bracon wasp and cotton aphid aphid wasp pupae; then the two specifications of the middle layer of the sieve are sieved twice from high to low, and the pupae left on the middle layer of the sieve are tobacco aphid bracon wasp, and the pupae that leak into the lower layer of the 40-mesh sieve are cotton aphid aphid wasp pupae; in this way, the pupae of the two parasitic wasps are separated and collected separately for later use. Example 2
[0010] A method for mixed breeding of two aphid parasitic wasps of the present invention comprises the following steps: The first step is to breed host plants Fill the small nutrient pot with nutrient soil to a height of 5 cm, then sow the eggplant seeds for seedling cultivation; when the eggplant seedlings are 5 cm tall, transplant them into the medium-sized seedling pot, spray water regularly, and set aside when the eggplant seedlings grow to 7 leaves; Step 2 Aphid inoculation and reproduction Aphids were inoculated, and the aphids were peach aphids. The leaves of the eggplant seedlings with aphids were cut into squares with a side length of 2 cm, and placed with the back side facing downward on the new eggplant seedlings, with 7 leaves placed on each plant; the single plant was placed in an insect cage with a 120-mesh gauze net, and the temperature in the artificial climate was 24°C and the humidity was 65%; after the aphids were transferred to the new eggplant seedlings, the square old leaves were removed; after 4.5 days, when the number of aphids on the new eggplant seedlings reached 900 per plant, the tobacco aphid braconid was inoculated; Step 3: Obtaining and inoculating two species of aphid parasitic wasps Bee pupae of two parasitic wasps, Aphididae fasciatus and Aphididae scoparia, were collected from eggplant vines, and large and brightly colored individuals were selected and placed in an insect culture tube. The lid of the insect culture tube was provided with a round hole, which was glued with a 100-mesh gauze. The insect culture tube was placed in a constant temperature box with a temperature of 25°C and a humidity of 70%, and the emergence of bee pupae was observed every day. Adult Aphididae fasciatus that emerged within 24 hours were placed in insect cages, and the inoculation density of Aphididae fasciatus was 45 per plant. Four days after the inoculation of Aphididae fasciatus, adult Aphididae scoparia that emerged within 24 hours were inoculated in insect cages again, and the inoculation density of Aphididae scoparia was 45 per plant. After inoculation, the insect cages were placed again, and the inoculation density of Aphididae scoparia that emerged within 24 hours was 45 per plant. After inoculation, the insects were placed in an artificial climate room with a temperature of 26.5°C and a humidity of 65%. Step 4: Collection of parasitic wasps When only white or light brown spherical pupae of Aphididae cirrhosae appeared on the leaves of the eggplant seedlings in the insect cage, start recording the time, wait for 2 days, and collect a large number of black pupae of Aphididae cirrhosae after they appeared; pick off the leaves carrying the two types of parasitic wasp pupae, and rinse them with clean water using a pressurized water gun to obtain the two types of parasitic wasp pupae; Step 5: Remove impurities The two types of parasitic wasp pupae that were washed down were repeatedly rinsed and separated to remove the heavier small soil pieces, sand and other impurities; the two types of parasitic wasp pupae were then placed on a gauze net to dry; after 1.5 days, the two types of parasitic wasp pupae were placed on a 40-mesh sieve to sieve out the fine impurities, and the two types of parasitic wasp pupae would not fall off; Step 6: Separation of two parasitic wasps The pupae of the two parasitic wasps are of different sizes, and the pupae of the tobacco aphid bracon wasp are larger than the pupae of the cotton aphid aphid wasp; the two pupae are separated by placing sieves of different meshes; a 40-mesh sieve is placed on the lower layer to ensure that the pupae that are sieved do not fall down, 25-mesh and 30-mesh sieves are placed on the middle layer to separate the smaller tobacco aphid bracon wasp and the cotton aphid aphid wasp pupae, and the top layer is a 20-mesh sieve to separate the larger tobacco aphid bracon wasp; sieve from top to bottom in sequence, first put the two pupae on the top layer of the sieve, sieve repeatedly twice, the pupae left on the top layer of the sieve are larger tobacco aphid bracon wasp, and the pupae that fall into the middle layer of the 25-mesh and 30-mesh sieves are smaller tobacco aphid bracon wasp and cotton aphid aphid wasp pupae; then the two specifications of the middle layer of the sieve are sieved 3 times from high to low, and the pupae left on the middle layer of the sieve are tobacco aphid bracon wasp, and the pupae that leak into the lower layer of the 40-mesh sieve are cotton aphid aphid wasp pupae; in this way, the pupae of the two parasitic wasps are separated and collected separately for later use. Example 3
[0011] A method for mixed breeding of two aphid parasitic wasps of the present invention comprises the following steps: The first step is to breed host plants Fill the small nutrient pot with nutrient soil to a height of 6 cm, then sow the eggplant seeds for seedling cultivation; when the eggplant seedlings are 6 cm tall, transplant them into the medium-sized seedling pot, spray water regularly, and set aside when the eggplant seedlings grow to 8 leaves; Step 2 Aphid inoculation and reproduction Aphids were inoculated, and the aphids were green peach aphids. The leaves of the eggplant seedlings with aphids were cut into squares with a side length of 2 cm, and placed with the back side facing downward on the new eggplant seedlings, with 8 leaves placed on each plant; the single plant was placed in an insect cage with a 120-mesh gauze net, and the temperature in the artificial climate was 28°C and the humidity was 80%; after the aphids were transferred to the new eggplant seedlings, the square old leaves were removed; after 5 days, when the number of aphids on the new eggplant seedlings reached 1,000 per plant, the tobacco aphid braconid was inoculated; Step 3: Obtaining and inoculating two species of aphid parasitic wasps Bee pupae of two parasitic wasps, Aphididae fasciatus and Aphididae scoparia, were collected from eggplant vines, and large and brightly colored individuals were selected and placed in an insect culture tube. The lid of the insect culture tube was provided with a round hole, which was glued with a 100-mesh gauze. The insect culture tube was placed in a constant temperature box with a temperature of 26°C and a humidity of 80%, and the emergence of the bee pupae was observed every day. Adult Aphididae fasciatus that emerged within 24 hours were placed in an insect cage, and the inoculation density of Aphididae fasciatus was 50 per plant. Four days after the inoculation of Aphididae fasciatus, adult Aphididae scoparia that emerged within 24 hours were inoculated in the insect cage again, and the inoculation density of Aphididae scoparia was 50 per plant. After the inoculation, the insect cage was placed in an artificial climate room with a temperature of 28°C and a humidity of 80%. Step 4: Collection of parasitic wasps When only white or light brown spherical pupae of Aphididae cirrhosae appeared on the leaves of the eggplant seedlings in the insect cage, start recording the time, wait for 3 days, and collect a large number of black pupae of Aphididae cirrhosae after they appeared; pick off the leaves carrying the two types of parasitic wasp pupae, and rinse them with clean water using a pressurized water gun to obtain the two types of parasitic wasp pupae; Step 5: Remove impurities The two types of parasitic wasp pupae that were washed down were repeatedly rinsed and separated to remove the heavier small soil pieces, sand and other impurities; the two types of parasitic wasp pupae were then placed on a gauze net to dry; after 2 days, the two types of parasitic wasp pupae were placed on a 40-mesh sieve to sieve out the fine impurities, and the two types of parasitic wasp pupae would not fall off; Step 6: Separation of two parasitic wasps The pupae of the two parasitic wasps are of different sizes, and the pupae of the tobacco aphid bracon wasp are larger than the pupae of the cotton aphid aphid wasp; the two pupae are separated by placing sieves of different meshes; a 40-mesh sieve is placed on the lower layer to ensure that the pupae that are sieved do not fall down, 25-mesh and 30-mesh sieves are placed on the middle layer to separate the smaller tobacco aphid bracon wasp and the cotton aphid aphid wasp pupae, and the top layer is a 20-mesh sieve to separate the larger tobacco aphid bracon wasp; sieve from top to bottom in sequence, first put the two pupae on the top layer of the sieve, sieve repeatedly twice, the pupae left on the top layer of the sieve are larger tobacco aphid bracon wasp, and the pupae that fall into the middle layer of the 25-mesh and 30-mesh sieves are smaller tobacco aphid bracon wasp and cotton aphid aphid wasp pupae; then the two specifications of the middle layer of the sieve are sieved 3 times from high to low, and the pupae left on the middle layer of the sieve are tobacco aphid bracon wasp, and the pupae that leak into the lower layer of the 40-mesh sieve are cotton aphid aphid wasp pupae; in this way, the pupae of the two parasitic wasps are separated and collected separately for later use.
[0012] The method for mixed breeding of two aphid parasitic wasps provided by the present invention is not limited to the above embodiments, and any modifications, equivalent substitutions and improvements made on the basis of the present invention should be included in the protection scope of the present invention.
Claims
1. A method for mixed rearing of two aphid parasitic wasps, characterized in that The steps include: The first step is to breed host plants Fill the small nutrient pot with nutrient soil to a height of 4-6 cm, then sow the eggplant seeds for seedling cultivation; when the eggplant seedlings are 4-6 cm tall, transplant them into the medium-sized seedling pot, spray water regularly, and set aside when the eggplant seedlings grow to 6-8 leaves; Step 2 Aphid inoculation and reproduction Inoculate aphids, the aphids are peach aphids, cut the eggplant vine leaves with aphids into squares with a side length of 2 cm, put them on the new eggplant vine plants with the back side facing down, and put 6-8 leaves on each plant; put the single plant in an insect cage with a 120-mesh gauze net, the temperature in the artificial climate is 20-28℃, and the humidity is 50-80%; after the aphids are transferred to the new eggplant vine plants, remove the square old leaves; after 4-5 days, when the number of aphids on the new eggplant vine plants reaches 800-1000 per plant, inoculate with tobacco aphid braconids; Step 3: Obtaining and inoculating two species of aphid parasitic wasps Bee pupae of two parasitic wasps, Aphididae fasciatus and Aphididae scoparia, were collected from eggplant vines, and large and brightly colored individuals were selected and placed in an insect culture tube. The lid of the insect culture tube was provided with a round hole, which was glued with a 100-mesh gauze. The insect culture tube was placed in a constant temperature box with a temperature of 24-26°C and a humidity of 60-80%, and the emergence of the bee pupae was observed every day. Adult Aphididae fasciatus that emerged within 24 hours were placed in an insect cage, and the inoculation density of Aphididae fasciatus was 40-50 heads / plant. Four days after the inoculation of Aphididae fasciatus, adult Aphididae scoparia that emerged within 24 hours were inoculated in the insect cage again, and the inoculation density of Aphididae scoparia was 40-50 heads / plant. After the inoculation, the insect cage was placed in an artificial climate room with a temperature of 25-28°C and a humidity of 50-80%. Step 4: Collection of parasitic wasps When only white or light brown spherical pupae of Aphididae cirrhosae appear on the leaves of the eggplant seedlings in the insect cage, start recording the time, wait for 2-3 days, and collect a large number of black pupae of Aphididae cirrhosae after they appear; pick off the leaves carrying the two types of parasitic wasp pupae, and rinse them with clean water using a pressurized water gun to obtain the two types of parasitic wasp pupae; Step 5: Remove impurities The two types of parasitic wasp pupae washed down are repeatedly rinsed and separated to remove the heavy small soil pieces, sand and other impurities; the two types of parasitic wasp pupae are then placed on a gauze net to dry; after 1-2 days, the two types of parasitic wasp pupae are placed on a 40-mesh sieve to sieve out the fine impurities, and the two types of parasitic wasp pupae will not fall off; Step 6: Separation of two parasitic wasps The pupae of the two parasitic wasps are different in size, with the pupae of the tobacco aphid wasp being larger than the pupae of the cotton aphid wasp. The pupae of the two parasitic wasps were separated by placing sieves of different mesh sizes. A 40-mesh sieve is placed on the lower layer to ensure that the bee pupae sieved through will not fall down. A 25-mesh and 30-mesh sieve is placed on the middle layer to separate the smaller pupae of Tobacco Aphid Bracone and Cotton Aphid Aphid Wasp. The top layer is a 20-mesh sieve to separate the larger ones. Sieve from top to bottom in sequence. First, put the two types of bee pupae on the top sieve and sieve them repeatedly for 2 times. The ones left on the top sieve are the larger ones, and those that fall into the 25-mesh and 30-mesh middle sieves are the smaller ones. The two specifications of the middle sieve are sieved 2-3 times from high to low. The ones left on the middle sieve are Tobacco Aphid Bracone pupae, and those that leak into the 40-mesh sieve on the lower layer are Cotton Aphid Aphid Wasp pupae. In this way, the bee pupae of the two parasitic bees are separated and collected separately for later use.
2. The method for mixed breeding of two aphid parasitic wasps according to claim 1, characterized in that: The small nutrient pot is a barrel-shaped pot with a diameter of 8 cm and a height of 7.5 cm. The medium seedling pot is a barrel-shaped pot with a diameter of 12 cm and a height of 7.5 cm. The insect cage is 50 cm long, 50 cm wide and 50 cm high. The diameter of the insect tube is 2.5 cm and the height is 8 cm. The diameter of the round hole of the insect tube cover is 1.5 cm. The rated current of the high-pressure water gun is 2.4m 3 / h, power 0.75kW.
Citation Information
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