Application of a formula of effective ingredients of Radix Angelicae Dahuricae in treating cardiovascular and cerebrovascular diseases caused by diabetes

Abstract

The present invention relates to the field of pharmaceutical technology, and more specifically, to the use of a formula of active ingredients of Gastrodia elata for treating cardiovascular and cerebrovascular diseases caused by diabetes. The formula comprises the following ingredients: n-butyl lactone, esculetin, lignoceric acid, monomelite glycoside, lonicerin A, hyoscyamine, berberine saponin II, palmatine, taxifolin, and angoside B. The drug prepared using this formula exhibits excellent therapeutic effects on cardiovascular and cerebrovascular diseases caused by diabetes.

Description

Technical Field

[0001] The present invention relates to the field of pharmaceutical technology, and more particularly to an application of a formula of effective ingredients of Gastrodia elata in treating cardiovascular and cerebrovascular diseases caused by diabetes. Background Art

[0002] Cardiovascular and cerebrovascular diseases (CVDs) are extremely common medical conditions in my country. They generally refer to common conditions related to the heart and brain blood vessels, primarily including coronary heart disease, cerebrovascular disease, hypertension, and heart disease. CVDs are characterized by high morbidity and mortality. Globally, they claim over 15 million lives each year, and the mortality rate is steadily increasing, posing a serious health threat, particularly to the elderly. The causes and mechanisms of CVDs remain unclear, but most researchers believe they are caused by chronic organ damage mediated by multiple factors, including diabetes, hypertension, and obesity.

[0003] Currently, clinical treatments for cardiovascular and cerebrovascular diseases mostly involve antihypertensive and hypoglycemic drugs, which can control symptoms. However, these drugs carry the risk of continued medication discontinuation and numerous adverse reactions. Traditional Chinese Medicine (TCM) offers the advantages of a multi-target, multi-component, and holistic approach to treating cardiovascular and cerebrovascular diseases. Zhang Xichun, a renowned modern TCM expert, proposed that "the human spirit originates in the heart and brain, and the function of the spirit is based on the harmonious interaction of the heart and brain." Traditional Chinese medicine theory posits that the etiology of cardiovascular and cerebrovascular diseases is characterized by "root deficiency and superficial excess," with the root being deficiency of heart and kidney yang and the superficial being phlegm, turbidity, and blood stasis. The primary treatment is with drugs that promote blood circulation and dissipate blood stasis. Guizhi Tangju Granules are composed of nine herbs: Angelica sinensis, Anemarrhena asphodeloides, Honeysuckle, Honeysuckle, Coptis chinensis, Rehmannia root, Scrophularia ningpoensis, Licorice root, and Polygonum multiflorum fruit. Angelica sinensis and Polygonum multiflorum fruit promote blood circulation and dissipate blood stasis, while Anemarrhena asphodeloides, Honeysuckle, Honeysuckle, Coptis chinensis, Rehmannia root, Scrophularia ningpoensis, and Licorice root clear heat, detoxify, and dissipate wind and unclog the meridians. In general, there is no relevant research on the application of the formula of effective ingredients of Guizhi Sugarcane in the preparation of treatment for cardiovascular and cerebrovascular diseases caused by diabetes. Summary of the Invention

[0004] In order to make up for the deficiencies of the prior art, the present invention provides a formula of effective ingredients of Radix Glycyrrhizae uralensis for use in treating cardiovascular and cerebrovascular diseases caused by diabetes.

[0005] The present invention is achieved through the following technical solutions:

[0006] In the first aspect, the present invention provides an application of an effective ingredient formula of Gastrodia elata in the treatment of cardiovascular and cerebrovascular diseases caused by diabetes, wherein the effective ingredient formula of Gastrodia elata comprises the following ingredients: n-butyl lactone, esculent acid, lignoceric acid, monomelitrin, honeysuckle glycoside A, cypermethrin, berberine saponin II, palmatine, taxifolin, and angoside B.

[0007] Preferably, the mass ratio of each component of the effective ingredient formula of the Chinese medicine for treating acne is: n-butyl lactone: esculent acid: lignoceric acid: monomelitrin: honeysuckle glycoside: cyperus rotundus: berberine: berberine: berberine: berberine saponin II: palmatine: taxifolin: angoside B: (3.8-4.6): (1.3-1.8): (0.9-1.3): (1.2-1.7): (1.0-1.6): (0.5-0.9): (2.5-3.4): (2.8-3.6): (2.1-2.8): (0.3-0.8).

[0008] Preferably, the preferred mass ratio of each component of the effective ingredient formula of Guizhi Sugarcane is: n-butyl lactone: esculent acid: lignoceric acid: monomelitrin: honeysuckle glycoside: cyperus rotundus: berberine: berberine: berberine saponin II: palmatine: taxifolin: angoside B is: 4.1:1.5:1.1:1.4:1.3:0.7:2.9:3.2:2.6:0.7.

[0009] Preferably, the formula of the effective ingredients of Gastrodia elata can enhance the free radical scavenging activity, reduce the damage of free radicals to myocardial tissue, and have a better protective effect on myocardial tissue.

[0010] Preferably, the formula of the effective ingredients of Guizhi sugar cane can reduce the abnormal elevation stage of the ST segment, decrease the SPK level, protect the SOD activity of the myocardial tissue, and have a good protective effect on the myocardial tissue.

[0011] In a second aspect, the present invention provides a drug for treating cardiovascular and cerebrovascular diseases, which includes a formula of effective ingredients of Rhizoma Cibotii, including the following ingredients: n-butyl lactone, esculent acid, lignoceric acid, monomelitrin, honeysuckle glycoside A, cypermethrin, berberine saponin II, palmatine, taxifolin, and angoside B.

[0012] Compared with the prior art, the present invention is beneficial in that: the present invention provides a formula of effective ingredients for treating cardiovascular and cerebrovascular diseases caused by diabetes, and a drug prepared using the formula, which has excellent therapeutic effects on cardiovascular and cerebrovascular diseases caused by diabetes. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] The present invention will be further described below with reference to the accompanying drawings.

[0014] Figure 1 Effects on ST segment of electrocardiogram in rats with acute myocardial ischemia;

[0015] Figure 2 Effects on serum MDA in rats with acute myocardial ischemia;

[0016] Figure 3 Effects on serum CPK in rats with acute myocardial ischemia;

[0017] Figure 4 Effects on serum SOD in rats with acute myocardial ischemia. DETAILED DESCRIPTION

[0018] The present invention is described in further detail below so that those skilled in the art can implement the invention with reference to the description.

[0019] It should be noted that the experimental methods described in the following embodiments are conventional methods unless otherwise specified, and the reagents and materials are commercially available unless otherwise specified.

[0020] Example 1: Formula of the active ingredient of Gyuzhi carbuncle

[0021] The mass ratios of the various components in the formula of the effective ingredients of Guizhi Sugarcane are: n-butyl lactone: esculent acid: lignoceric acid: monomelitrin: honeysuckle glycoside: cyperus rotundus: berberine: berberine: berberine saponin II: palmatine: tachyphyllin: angoside B: (3.8-4.6): (1.3-1.8): (0.9-1.3): (1.2-1.7): (1.0-1.6): (0.5-0.9): (2.5-3.4): (2.8-3.6): (2.1-2.8): (0.3-0.8).

[0022] In this embodiment, the mass ratio of the effective ingredient formula of Guizhi Sugarcane is n-butyl lactone: esculent acid: lignoceric acid: monomelitrin: honeysuckle glycoside: cyperus rotundus: berberine: berberine: berberine: berberine: berberine: 4.1:1.5:1.1:1.4:1.3:0.7:2.9:3.2:2.6:0.7.

[0023] Example 2: Experimental study on the treatment of acute myocardial ischemia caused by diabetes mellitus using Guizhi Tangju Granules in rats

[0024] 1. Materials

[0025] 1.1 Experimental Animals

[0026] Sixty male SPF Wistar rats weighing 150-200 g were provided by Chengdu Dashuo Experimental Animal Co., Ltd. All experimental animals were housed in a controlled environment with a room temperature of 20-25°C and a humidity of 40%-50%. During the experiment, the animals had free access to food and water, and their circadian rhythms were normal.

[0027] 1.2 Main drugs and reagents

[0028] Guizhi Tanghu Granules (prepared into granules according to the mass ratio of Example 1); diltiazem hydrochloride: 0.3 mg / kg,

[0029] Purchased from Tianjin Tanabe Pharmaceutical Co., Ltd.

[0030] 2. Experimental process

[0031] 2.1 Modeling and grouping

[0032] After two weeks of adaptive feeding, 60 male Wistar rats were randomly selected from the control group (15 rats) and the remaining 45 rats were randomly assigned to the diabetic cardiovascular and cerebrovascular disease model. The rats were divided into a model group, a traditional Chinese medicine group, and a Western medicine group, with 15 rats in each group. Myocardial ischemia was induced by subcutaneous injection of 30 mg / kg of isoproterenol. Electrocardiograms (ECGs) were recorded before drug administration in each group. Six hours after administration of isoproterenol or saline, ECGs were repeated, and femoral artery blood was collected and the hearts were removed for homogenate preparation.

[0033] 2.2 Dosage

[0034] Drugs were administered 2 hours after modeling.

[0035] The model group and the normal group were gavaged with normal saline (30 mg / kg).

[0036] The Chinese medicine group was given Guizhi Tangju Granules aqueous solution (concentration 30 mg / kg) by gavage.

[0037] Western medicine group: diltiazem hydrochloride was added to distilled water to prepare a 20ug / ml suspension and administered orally.

[0038] 2.3 Electrocardiogram measurement

[0039] ST segment deviation is an indicator of the degree of myocardial ischemia and a measure of drug efficacy. In this study, normal lead II electrocardiograms were recorded for each group of animals. The total millivolt value of ST segment deviation after administration of isoproterenol was measured. The number of animals with abnormal ST segment deviation was recorded as the positive value, and the mean deviation value was recorded.

[0040] 2.4 Preparation of myocardial homogenate and determination of serum creatine phosphokinase (CPK), superoxide dismutase (SOD), and lipid peroxide malondialdehyde (MDA)

[0041] After removing the heart, remove any residual blood, weigh it, place it in normal saline below 0°C, and grind it at 4°C to make a 20% homogenate. CPK was measured using an automated biochemical analyzer; SOD in myocardial homogenate was measured using a modified pyrogallol autooxidation method. MDA was measured spectrophotometrically.

[0042] 3. Results

[0043] 3.1 Effects on the ST segment of the electrocardiogram in rats with acute myocardial ischemia

[0044] The results of the effects of the drug of the present invention on the ST segment of the electrocardiogram of rats with acute myocardial ischemia are shown in Table 1 below. Compared with the normal group, the ST segment deviation in the model group was significantly increased. Compared with the model group, the ST segment deviation in the Chinese medicine group and the Western medicine group was significantly reduced.

[0045] Table 1 Effects on ST segment of electrocardiogram in rats with acute myocardial ischemia (n=15)

[0046]

[0047] Note: *Compared with the normal group (p < 0.05); #Compared with the model group (p < 0.05)

[0048] 3.2 Effects on serum MDA, CPK, and SOD in rats with acute myocardial ischemia

[0049] Table 2 Effects on MDA, CPK and SOD in rats with acute myocardial ischemia (n=15)

[0050]

[0051] Note: *Compared with the normal group (p < 0.05); #Compared with the model group (p < 0.05)

[0052] The effects of the drugs of the present invention on serum MDA, CPK, and SOD in rats with acute myocardial ischemia are shown in Table 2. Compared with the normal group, the model group showed significantly increased myocardial tissue c(MDA) / nmol*L-1 concentrations and CPK concentrations, while significantly decreased SOD concentrations. Compared with the model group, the Chinese medicine group and the Western medicine group showed significantly decreased myocardial tissue c(MDA) / nmol*L-1 concentrations and CPK concentrations, while significantly increased SOD concentrations.

[0053] The present invention uses isoproterenol to induce acute myocardial ischemia in rats, and the electrocardiogram ST segment abnormality increases, serum CPK activity increases, MDA concentration increases, and the free radical scavenger SOD activity decreases. The Guizhi Sugar Urticaria Granules of the present invention (prepared by the mass ratio of Example 1) can reduce the stage of abnormal ST segment elevation and SPK levels, and have a good protective effect on myocardial tissue. In addition, this medicine can protect myocardial tissue SOD activity, enhance free radical scavenging activity, and can alleviate the damage of free radicals to myocardial tissue.

Claims

1. Use of a formula of effective ingredients of Gyuzhi sugar carbuncle in the preparation of a medicament for treating acute myocardial ischemia caused by diabetes, characterized in that: The effective ingredient formula of the Guizhi sugar carbuncle comprises the following components: n-Butyl lactone, esculetin, lignoceric acid, monomelite glycoside, honeysuckle glycoside A, cypermethrin, berberine saponin II, palmatine, taxifolin, and angoside B; wherein the mass ratio of each component of the active ingredient formula of saccharomyces cerevisiae is: n-Butyl lactone: esculetin: lignoceric acid: monomelite glycoside: honeysuckle glycoside: cypermethrin: berberine saponin II: palmatine: taxifolin: angoside B is: 4.1:1.5:1.1:1.4:1.3:0.7:2.9:3.2:2.6:0.7。 2. The use according to claim 1, characterized in that: The formula of the effective ingredients of Gastrodia elata can enhance the free radical scavenging activity, reduce the damage of free radicals to myocardial tissue, and have a good protective effect on myocardial tissue.

3. The use according to claim 1, characterized in that: The formula of Guizhi Sugarcane's active ingredients can reduce the abnormal elevation of the ST segment, lower the SPK level, protect the SOD activity of myocardial tissue, and have a good protective effect on myocardial tissue.

4. A pharmaceutical composition for treating acute myocardial ischemia caused by diabetes, characterized in that: The active ingredient comprises the formula of effective ingredients of Gastrodia elata in the mass ratio as claimed in claim 1.

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