Columnar algae-bacterium biological photovoltaic device system, coupling power generation method and application

Abstract

The invention provides a columnar algae-bacterium biological photovoltaic device system, a coupling power generation method and application, the columnar algae-bacterium biological photovoltaic device system comprises a main body and a light source, the main body comprises a first chamber, a spacing layer and a second chamber which are sequentially arranged from top to bottom, the first chamber is used for filling algae liquid, the spacing layer is used for filling algae liquid, and the second chamber is used for filling algae liquid; a light source is arranged above the first chamber and is used for irradiating the algae liquid; hydrogel is arranged in the spacing layer; the second chamber is used for filling bacterial liquid; a first electrode and a second electrode are arranged in the inner cavity of the first cavity and the inner cavity of the second cavity respectively, and the first electrode is electrically connected with the second electrode. The photosynthetic process of the algae liquid and the electrogenesis process of the bacteria liquid can be effectively coupled, so that the photoelectric process in a lake ecosystem is simulated.

Description

Technical Field

[0001] The present invention belongs to the technical field of renewable energy, and relates to a columnar algae-bacteria bio-photovoltaic device system, a coupled power generation method and applications. Background Art

[0002] The basic energy source for human activities comes from the sun, and solar energy can be converted and stored through photosynthesis and other forms of plants. Biophotovoltaics (BPV) is a technology that uses photosynthesis or bioelectrochemical processes of microorganisms to convert solar energy into chemical energy or electrical energy. Early BPV research focused on the effects of factors such as microbial species, light intensity, and electron carrier types and concentrations on photocurrent. There is no consensus on which microorganisms perform best in the BPV system and what the molecular mechanism of photosynthetic electron transfer is.

[0003] Recent studies have shown that the microbial photoelectric coupling process in the BPV system can exist naturally in aquatic ecosystems and has great potential to contribute to biogeochemical cycles. However, the carbon flux in the BPV system, the influencing factors, and its role in the carbon cycle of shallow lakes are still unknown.

[0004] Lakes are important aquatic ecosystems, and their carbon flux has become a frontier and hot topic in global carbon cycle research. In-depth analysis and clarification of the carbon cycle process in lake environments is crucial to improving global carbon cycle models and predicting the feedback of lake environments to climate change. Summary of the invention

[0005] In view of the shortcomings of the prior art, the purpose of the present invention is to provide a columnar algae-bacteria biophotovoltaic device system, a coupled power generation method and application, which can effectively couple the photosynthetic process of the algae liquid and the power generation process of the bacterial liquid, thereby simulating the photoelectric process in the lake ecosystem and realizing the analysis of the carbon cycle process.

[0006] To achieve this object, the present invention adopts the following technical solutions:

[0007] In the first aspect, the present invention provides a columnar algae-bacteria biophotovoltaic device system, including a main body and a light source, the main body including a first chamber, a spacer layer and a second chamber arranged in sequence from top to bottom, the first chamber is used to fill with algae liquid, and the light source is used to irradiate the algae liquid in the first chamber; the spacer layer is provided with a hydrogel; the second chamber is used to fill with bacterial liquid; the inner cavities of the first chamber and the second chamber are also respectively provided with a first electrode and a second electrode, and the first electrode is electrically connected to the second electrode.

[0008] As a preferred technical solution of the present invention, the first chamber is externally connected to a circulation device for internal circulation of the algae liquid.

[0009] Preferably, the circulation device includes a feed pipe, a discharge pipe and a circulation pump assembly, one end of the feed pipe is connected to the top of the first chamber, and the other end is connected to the circulation pump assembly, one end of the discharge pipe is connected to the bottom of the first chamber, and the other end is connected to the circulation pump assembly.

[0010] Preferably, the first chamber is also connected to a carbon dioxide detection component.

[0011] The top of the first chamber of the present invention is sealed, and the algae liquid is kept uniformly mixed by internal circulation, so that the algae liquid can photosynthesize more fully to fix carbon dioxide and produce organic matter.

[0012] As a preferred technical solution of the present invention, the spacer layer further comprises a porous partition, and the hydrogel is fixed on the porous partition.

[0013] In the present invention, the hydrogel completely wraps the pores of the porous partition during the solidification process, thereby preventing the problem of liquid leakage between the first chamber and the second chamber during operation.

[0014] Preferably, the pore size of the porous partition is 1.0-2.0 mm, for example, it can be 1.0 mm, 1.1 mm, 1.2 mm, 1.3 mm, 1.4 mm, 1.5 mm, 1.6 mm, 1.7 mm, 1.8 mm, 1.9 mm or 2.0 mm, but it is not limited to the listed values, and other unlisted values ​​within the numerical range are also applicable.

[0015] The present invention can not only ensure the transmission of organic matter but also avoid liquid leakage between the upper and lower chambers by maintaining the pore size of the porous partition within the above range.

[0016] As a preferred technical solution of the present invention, a stirring device is provided at the bottom of the second chamber.

[0017] The present invention provides a stirring device to maintain uniform mixing of the bacterial solution, so that the fungi can evenly contact the organic matter and oxidize it to generate electrons, which are transferred to the second electrode and then transmitted to the first electrode through an external circuit, thereby realizing a complete photoelectric coupling process.

[0018] Preferably, the stirring device comprises a magnetic stirrer.

[0019] Preferably, the second chamber is provided with a sampling port, and a dissolved oxygen detection component is arranged in the sampling port.

[0020] As a preferred technical solution of the present invention, the columnar algae-bacteria bio-photovoltaic device system further includes an online data collection device, and the online data collection device is electrically connected to the first electrode and the second electrode respectively.

[0021] Preferably, the first electrode and the second electrode independently include any one of graphite, carbon brush, carbon felt, carbon paper and carbon cloth, or a combination of at least two of them.

[0022] In a second aspect, the present invention provides a coupled power generation method of the columnar algae-bacteria bio-photovoltaic device system according to the first aspect, the coupled power generation method comprising:

[0023] A spacer layer is prepared in advance, the bacterial solution is filled into the second chamber, and the spacer layer is placed above the second chamber so that the hydrogel is in contact with the surface of the bacterial solution;

[0024] placing a first chamber above the hydrogel, filling the first chamber with algae solution, and electrically connecting the first electrode to the second electrode;

[0025] Light is provided to promote the algae liquid in the first chamber to photosynthesize, and the generated organic matter passes through the hydrogel into the second chamber, so as to form a voltage between the first electrode and the second electrode.

[0026] As a preferred technical solution of the present invention, the hydrogel is prepared under a sterile environment.

[0027] The hydrogel used in the present invention has biocompatibility, avoids the slow release of chemical substances that have adverse effects on biological systems, and is temperature-sensitive, and can completely wrap the pores in the porous partition during the natural cooling solidification process, solving the problem of leakage between the first chamber and the second chamber. The hydrogel can be prepared using agarose and gelatin commonly used in the art to maintain a solidified state within the temperature range of system operation.

[0028] Preferably, the thickness of the hydrogel is 4 to 5 mm, for example, 4 mm, 4.2 mm, 4.3 mm, 4.4 mm, 4.5 mm, 4.5 mm, 4.6 mm, 4.8 mm or 5 mm, but is not limited to the listed values, and other unlisted values ​​within the numerical range are also applicable.

[0029] As a preferred technical solution of the present invention, the bacterial liquid is an electroactive microbial culture liquid.

[0030] Preferably, the OD of the bacterial solution is 600 It is 0.8 to 1.0, for example, it can be 0.8, 0.81, 0.82, 0.85, 0.86, 0.88, 0.9, 0.92, 0.94, 0.95, 0.96, 0.98 or 1.0, but is not limited to the listed values, and other unlisted values ​​within the numerical range are also applicable.

[0031] Preferably, the culture temperature of the electroactive microorganisms is 25-30°C, for example, 25°C, 26°C, 27°C, 28°C, 29°C or 30°C, but is not limited to the listed values, and other unlisted values ​​within the numerical range are also applicable.

[0032] Preferably, the coupled power generation method further comprises: stirring the bacterial liquid in the second chamber.

[0033] Preferably, the stirring speed is 200-400 rpm, for example, it can be 200 rpm, 220 rpm, 240 rpm, 250 rpm, 280 rpm, 300 rpm, 320 rpm, 340 rpm, 350 rpm, 360 rpm, 380 rpm or 400 rpm, but is not limited to the listed values, and other unlisted values ​​within the numerical range are also applicable.

[0034] As a preferred technical solution of the present invention, the OD of the algae solution 730 It is 0.6 to 0.8, for example, it can be 0.6, 0.61, 0.62, 0.65, 0.66, 0.68, 0.7, 0.72, 0.74, 0.75, 0.76, 0.78 or 0.8, but is not limited to the listed values, and other unlisted values ​​within the numerical range are also applicable.

[0035] Preferably, the temperature of the algae liquid is 25-30°C, for example, 25°C, 26°C, 27°C, 28°C, 29°C or 30°C, but is not limited to the listed values, and other unlisted values ​​within the numerical range are also applicable.

[0036] Preferably, the coupled power generation method further comprises: internally circulating the algae liquid in the first chamber.

[0037] Preferably, the flow rate of the internal circulation is 140-160 mL / min, for example, it can be 140 mL / min, 142 mL / min, 145 mL / min, 146 mL / min, 148 mL / min, 150 mL / min, 153 mL / min, 155 mL / min, 156 mL / min, 158 mL / min or 160 mL / min, but is not limited to the listed values, and other unlisted values ​​within the numerical range are also applicable.

[0038] Preferably, the intensity of the light is 1500-2500Lx, for example, it can be 1500Lx, 1600Lx, 1700Lx, 1800Lx, 1900Lx, 2000Lx, 2050Lx, 2100Lx, 2150Lx, 2200Lx, 2250Lx, 2300Lx, 2350Lx, 2400Lx, 2450Lx or 2500Lx, but is not limited to the listed values, and other unlisted values ​​within the numerical range are also applicable.

[0039] In a third aspect, the present invention provides an application of the columnar algae-bacteria bio-photovoltaic device system described in the first aspect, wherein the columnar algae-bacteria bio-photovoltaic device system is used to simulate photoelectric processes in a lake ecosystem.

[0040] The numerical range described in the present invention not only includes the point values ​​listed above, but also includes any point values ​​between the above numerical ranges that are not listed. Due to space limitations and for the sake of simplicity, the present invention no longer exhaustively lists the specific point values ​​included in the range.

[0041] Compared with the prior art, the present invention has the following beneficial effects:

[0042] The present invention provides a columnar algae-bacteria biophotovoltaic device system, coupled power generation method and application, which utilizes algae liquid to fix CO2 through photosynthesis and produce organic matter. The organic matter can enter the bacterial liquid through the hydrogel between the upper and lower chambers. The bacterial liquid then uses the organic matter as an electron donor to oxidize it and produce electrons, which are transferred to the second electrode and then to the first electrode through an external circuit, thereby realizing the photosynthetic power generation coupling of the entire system. It can be used to simulate the photoelectric process in a lake environment, and provides an effective research device for studying the photoelectric process in a lake ecosystem. BRIEF DESCRIPTION OF THE DRAWINGS

[0043] Figure 1 A schematic structural diagram of a columnar algae-bacteria biophotovoltaic device system provided in an embodiment of the present invention.

[0044] Among them, 100-first chamber; 200-second chamber; 1-light source; 2-circulation device; 3-carbon dioxide sensor; 4-carbon dioxide detector; 5-hydrogel; 6-porous partition; 7-magnetic stirrer; 8-DO electrode; 9-dissolved oxygen meter; 10-first electrode; 11-second electrode; 12-online acquisition device. DETAILED DESCRIPTION

[0045] It should be understood that, in the description of the present invention, the terms "center", "upper", "lower", "front", "back", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating the orientation or positional relationship are based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", etc. are only used for descriptive purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, features defined as "first", "second", etc. may explicitly or implicitly include one or more of the features. In the description of the present invention, unless otherwise specified, "multiple" means two or more.

[0046] It should be noted that in the description of the present invention, unless otherwise clearly specified and limited, the terms "disposed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood by specific circumstances.

[0047] The technical solution of the present invention is further described below with reference to the accompanying drawings and through specific implementation methods.

[0048] In a specific embodiment, the present invention provides a columnar algae-bacteria biophotovoltaic device system, including a main body and a light source, wherein the main body includes a first chamber, a spacer layer, and a second chamber arranged in sequence from top to bottom. The first chamber is used to fill the algae liquid, and the light source is used to irradiate the algae liquid in the first chamber to perform photosynthesis of algae, fix CO2, and produce organic matter. A hydrogel is arranged in the spacer layer to allow organic matter to pass through so as to transfer the organic matter produced in the first chamber to the second chamber. The second chamber is used to fill the bacterial liquid, oxidize the organic matter, and generate electrons. The inner cavity of the first chamber is provided with a first electrode as a cathode, and the inner cavity of the second chamber is provided with a second electrode as an anode. The first electrode is electrically connected to the second electrode, and the second electrode receives the electrons provided by the bacterial liquid, and transmits them to the first electrode through an external circuit to form a voltage to realize the power generation process, that is, coupling the photosynthesis-power generation of algae-bacteria, which can simulate the photoelectric process in the lake.

[0049] In some embodiments, the first chamber is externally connected to a circulation device for internally circulating the algae liquid to maintain mixing of the algae liquid, ensure sufficient exposure to light for photosynthesis, and also help maintain the temperature of the algae liquid.

[0050] Specifically, the circulation device includes a feed pipe, a discharge pipe and a circulation pump assembly, one end of the feed pipe is connected to the top of the first chamber, and the other end is connected to the circulation pump assembly, one end of the discharge pipe is connected to the bottom of the first chamber, and the other end is connected to the circulation pump assembly. The circulation pump assembly can adopt a peristaltic pump commonly used by technicians in this field. In addition, the circulation device must include necessary pipelines, conventional valves and general pump equipment for realizing the complete process, but the above content does not belong to the main inventive point of the invention. Those skilled in the art can add layouts on their own based on the process flow and equipment structure selection, and the present invention does not make special requirements and specific limitations on this.

[0051] In some embodiments, the first chamber is further connected to a carbon dioxide detection component for real-time monitoring of CO2 concentration changes in the first chamber. Specifically, the carbon dioxide detection component may include an electrically connected carbon dioxide sensor and a carbon dioxide detector, the top of the first chamber is closed, the carbon dioxide sensor is inserted into the inner cavity of the first chamber, and the carbon dioxide detector is externally placed to detect the CO2 concentration, so that the staff can understand the photosynthesis of the algae liquid.

[0052] In some embodiments, the light source can be disposed on the outside of the main body, or can be disposed in the inner cavity of the first chamber, and the present invention does not specifically limit this. Specifically, when the light source is external, the main body can be made of a transparent material so that the light can fully illuminate the algae liquid. The light source can be a lamp tube, lamp bead or lamp board commonly used by technicians in this field. In addition, those skilled in the art can also place the entire device system in a light incubator according to actual needs. According to the operating conditions, adjust the light intensity and temperature to ensure that the first chamber and the second chamber are in a suitable light intensity and temperature range, which is conducive to the efficient operation of the photosynthesis-electricity production process.

[0053] In some embodiments, the spacer layer further comprises a porous separator, the hydrogel is fixed on the porous separator, and separation is performed by the combination of the hydrogel and the porous separator to achieve effective transmission of organic matter. Specifically, the pore size of the porous separator is 1.0 to 2.0 mm.

[0054] The hydrogel of the present invention needs to be prepared in advance and located between the upper and lower chambers, and the structure and size of the hydrogel are ensured to completely cover the liquid surface. In addition, the hydrogel can completely wrap the pores of the porous partition during the solidification process to prevent leakage between the upper and lower chambers.

[0055] In the present invention, the first chamber is detachably connected to the second chamber, ensuring that the spacer layer is located between the first chamber and the second chamber, and the first chamber and the second chamber are sealed, so that the staff can fill and replace the algae liquid and the bacterial liquid. The present invention does not specifically limit the connection method between the first chamber and the second chamber, and can adopt threaded connection, slot connection, bolt connection or buckle connection commonly used by those skilled in the art.

[0056] The first chamber and the second chamber are both columnar structures, which can be cylindrical or square. The present invention does not specifically limit the size of the first chamber and the second chamber, and those skilled in the art can adjust them according to the actual situation.

[0057] In some embodiments, a stirring device is provided at the bottom of the second chamber to maintain uniform mixing of the bacterial liquid in the second chamber. Specifically, the stirring device may be a magnetic stirrer, and its stirring speed may be controlled to avoid damaging the fungi.

[0058] In some embodiments, the second chamber is provided with a sampling port, and a dissolved oxygen detection component is disposed in the sampling port to monitor the change of dissolved oxygen content in the second chamber in real time. The dissolved oxygen detection component may include an electrically connected DO electrode and a dissolved oxygen meter, the DO electrode is inserted into the sampling port, and the interface thereof is sealed with glue, and the dissolved oxygen meter is externally placed to detect the dissolved oxygen concentration, so that the staff can grasp the oxygen level in the second chamber.

[0059] In some embodiments, the columnar algae-bacteria bio-photovoltaic device system further comprises an online collection device, which is electrically connected to the first electrode and the second electrode respectively. The first electrode and the second electrode are electrically connected to a resistor via a titanium wire, and the online collection device is connected to both ends of the resistor in the external circuit to collect the voltage. Specifically, the first electrode and the second electrode independently include any one or a combination of at least two of graphite, carbon brush, carbon felt, carbon paper and carbon cloth.

[0060] In another specific embodiment, the present invention provides a coupled power generation method of a columnar algae-bacteria bio-photovoltaic device system according to a specific embodiment, and the coupled power generation method comprises:

[0061] S1: Prepare a spacer layer in advance, fill the second chamber with bacterial liquid, and place the spacer layer above the second chamber so that the hydrogel fits the surface of the bacterial liquid.

[0062] Specifically, the hydrogel is prepared in a sterile environment, for example, in a clean bench. The thickness of the hydrogel is 4 to 5 mm. The hydrogel is prepared from agarose and gelatin, has biocompatibility and temperature sensitivity, can completely wrap the pores of the porous partition, and maintain a solidified state within the temperature range of the system operation. Preferably, the mass concentration of agarose and gelatin in the hydrogel is 3%.

[0063] Specifically, the bacterial solution includes electroactive microorganisms, and only a culture medium is provided, without providing an additional organic substrate as an electron donor for the electroactive microorganisms. When simulating the photoelectric process of a lake, a plurality of electroactive microorganisms can be directly screened from the lake environment and mixed to be added to the second chamber as a bacterial solution. In addition, the OD of the bacterial solution is 600 is 0.8-1.0, and the culture temperature of the electroactive microorganisms is 25-30°C.

[0064] Furthermore, the coupled power generation method further comprises: stirring the bacterial solution in the second chamber to maintain the bacterial solution mixed evenly. Specifically, the stirring speed is 200-400 rpm.

[0065] S2: placing the first chamber above the hydrogel, filling the first chamber with algae solution, and electrically connecting the first electrode to the second electrode.

[0066] The algae liquid can be any algae that can achieve photosynthesis known to those skilled in the art, and the present invention does not specifically limit this. When simulating the photoelectric process of a lake, algae can be directly selected from the lake environment and mixed to be added to the first chamber as the algae liquid. In addition, the OD of the algae liquid is 730 is 0.6-0.8, and the temperature of the algae liquid is 25-30°C.

[0067] Furthermore, the coupled power generation method further comprises: performing internal circulation on the algae liquid in the first chamber to maintain the algae liquid mixing. Specifically, the flow rate of the internal circulation is 140-160 mL / min.

[0068] S3: providing light to promote the algae liquid in the first chamber to photosynthesize, and the generated organic matter passes through the hydrogel into the second chamber, so as to form a voltage between the first electrode and the second electrode.

[0069] Specifically, the intensity of the light is 1500-2500Lx.

[0070] All culture media, consumables, and devices involved in the present invention need to be sterilized. For parts that cannot be sterilized by high temperature and high pressure (such as CO2 sensors, DO electrodes, etc.), sterility is ensured by ultraviolet sterilization + alcohol disinfection.

[0071] The operation process of the columnar algae-bacteria biophotovoltaic device system provided by the present invention includes: after the device system is built according to the above-mentioned coupled power generation method, it is placed under a light source to provide illumination, the algae liquid in the first chamber fixes CO2 through photosynthesis and produces organic matter, and the organic matter can pass through the hydrogel between the upper and lower chambers into the second chamber of the lower layer; the electroactive microorganisms in the second chamber use the permeated organic matter as an electron donor, oxidize it and generate electrons, transfer them to the second electrode serving as the anode, and then transfer them to the first electrode serving as the cathode through an external circuit; the output voltage of the device system is monitored in real time by an online acquisition device; the CO2 content change in the first chamber is monitored by a carbon dioxide detection component, and the DO content change in the second chamber is monitored by a dissolved oxygen detection component.

[0072] Example

[0073] This embodiment provides a columnar algae-bacteria bio-photovoltaic device system for simulating the photovoltaic process in a lake ecosystem. Figure 1 As shown, the columnar algae-bacteria biophotovoltaic device system provided in this embodiment includes a first chamber 100, a spacer layer and a second chamber 200 arranged in sequence from top to bottom. The first chamber 100 is filled with algae liquid, and a light source 1 is arranged above it for irradiating the algae liquid. The first chamber 100 is externally connected to a circulation device 2 for internal circulation of the algae liquid. The top of the first chamber 100 is closed, and a carbon dioxide sensor 3 is inserted into the inner cavity of the first chamber, and is electrically connected to an external carbon dioxide detector 4 for detecting changes in carbon dioxide concentration in the first chamber 100. A hydrogel 5 and a porous partition 6 are arranged in the spacer layer, and the pore size of the porous partition 6 is 1 mm. The hydrogel 5 is fixed on the porous partition 6, and the solidified hydrogel 5 covers the pores of the porous partition 6. The second chamber 200 is filled with bacterial liquid, and a magnetic stirrer 7 is arranged at the bottom of the second chamber 200 for stirring the bacterial liquid. The second chamber 200 is provided with a sampling port, in which a DO electrode 8 is arranged, and is electrically connected to an external dissolved oxygen meter 9, for detecting the change of dissolved oxygen concentration in the second chamber 200. The inner cavities of the first chamber 100 and the second chamber 200 are also provided with a first electrode 10 and a second electrode 11, respectively, both of which are made of carbon felt, and the first electrode 10 is electrically connected to the second electrode 11 through a titanium wire and a resistor. The external circuit between the first electrode 10 and the second electrode 11 is connected to an online acquisition device 12 for voltage acquisition. The columnar algae-bacteria bio-photovoltaic device system also includes a temperature control device.

[0074] The coupled power generation method of the columnar algae-bacteria bio-photovoltaic device system provided in this embodiment includes:

[0075] (1) Pre-prepare the hydrogel in an ultra-clean workbench to ensure that the hydrogel is of a size that can be placed between the first chamber and the second chamber and completely covers the pores of the porous partition during the solidification process;

[0076] (2) manually adding bacterial solution into the second chamber, and after the chamber is filled, placing the spacer layer on top of the second chamber so that the hydrogel is in contact with the surface of the bacterial solution;

[0077] (3) placing the first chamber on top of the hydrogel, fixing and tightening the first chamber and the second chamber, and then filling the first chamber with algae liquid;

[0078] (4) electrically connecting the first electrode to the second electrode;

[0079] (5) Starting the internal circulation of the first chamber to maintain the uniform mixing of the algae solution, and simultaneously starting the stirring of the second chamber to maintain the uniform mixing of the bacterial solution;

[0080] (6) providing light to promote the algae liquid in the first chamber to photosynthesize and produce organic matter that passes through the hydrogel into the second chamber, thereby forming a voltage between the first electrode and the second electrode;

[0081] (7) The output voltage of the system is monitored in real time by an online data acquisition device, the CO2 content change in the first chamber is monitored in real time by a carbon dioxide sensor, the DO content change in the second chamber is monitored by a DO electrode, and the temperature of the first chamber and the second chamber is adjusted by a temperature control device.

[0082] In order to better fit the photoelectric process in the lake ecosystem, the hydrogel used in this embodiment is screened. A 100 mg / L TOC (Total Organic Carbon) standard solution is filled into the first chamber, and pure water is filled into the second chamber, and the two chambers are separated by hydrogels. The change of TOC in the second chamber over time when a hydrogel with a thickness of 4 to 5 mm is used is compared, and the hydrogel with the best TOC permeability is selected.

[0083] The toughness, strength and morphology retention of hydrogels with different mass concentrations of agarose and gelatin (1%, 1.5%, 3% and 4.5%) were compared. Through appearance observation and hardness touch, it was found that the hydrogel with agarose and gelatin mass concentration of 3% was the least fragile, with a smoother surface and higher toughness.

[0084] Application Examples

[0085] This application example uses the columnar algae-bacteria biophotovoltaic device system provided in the embodiment to simulate the photoelectric process in the lake ecosystem. In the first chamber, the algae liquid fixes CO2 through photosynthesis and produces organic matter, which can pass through the hydrogel between the upper and lower chambers into the lower second chamber. The electroactive microorganisms in the second chamber use the organic matter that passes through the photosynthesis of the upper layer as electron donors, oxidize it to produce electrons, transfer them to the second electrode, and then transfer them to the first electrode through the external circuit. The voltage change is recorded every 10 minutes using an online acquisition device to obtain the output voltage change. The CO2 content data is recorded every 10 minutes using the carbon dioxide sensor in the first chamber to obtain the CO2 content change curve. The DO content data in the second chamber is recorded every 12 hours using the DO electrode in the second chamber to obtain the DO content change curve. At the same time, the TOC changes in the first and second chambers are sampled and measured every 12 hours to obtain the TOC content change curve. The OD values ​​of the upper algae liquid and the lower bacterial liquid are sampled and measured every day to obtain the OD concentration curve. Ensure that the entire system can maintain a relatively stable photosynthetic electricity coupling activity during operation, and both algae and bacteria maintain good metabolism.

[0086] The applicant declares that the above is only a specific implementation mode of the present invention, but the protection scope of the present invention is not limited thereto. Those skilled in the art should understand that any changes or substitutions that can be easily thought of by those skilled in the art within the technical scope disclosed by the present invention shall fall within the protection scope and disclosure scope of the present invention.

Claims

1. A columnar algae-bacteria bio-photovoltaic device system, characterized in that: The invention comprises a main body and a light source, wherein the main body comprises a first chamber, a spacer layer and a second chamber arranged in sequence from top to bottom, the first chamber is used to be filled with algae liquid, and the light source is used to irradiate the algae liquid in the first chamber; a hydrogel is arranged in the spacer layer; the second chamber is used to be filled with bacterial liquid; the inner cavities of the first chamber and the second chamber are also respectively provided with a first electrode and a second electrode, and the first electrode is electrically connected to the second electrode.

2. The columnar algae-bacteria bio-photovoltaic device system according to claim 1, characterized in that: The first chamber is externally connected to a circulation device for internal circulation of the algae liquid; Preferably, the circulation device comprises a feed pipe, a discharge pipe and a circulation pump assembly, one end of the feed pipe is connected to the top of the first chamber, and the other end is connected to the circulation pump assembly, one end of the discharge pipe is connected to the bottom of the first chamber, and the other end is connected to the circulation pump assembly; Preferably, the first chamber is also connected to a carbon dioxide detection component.

3. The columnar algae-bacteria bio-photovoltaic device system according to claim 1 or 2, characterized in that: The spacer layer further comprises a porous separator, and the hydrogel is fixed on the porous separator; Preferably, the pore size of the porous partition is 1.0-2.0 mm.

4. The columnar algae-bacteria bio-photovoltaic device system according to any one of claims 1 to 3, characterized in that: A stirring device is provided at the bottom of the second chamber; Preferably, the stirring device comprises a magnetic stirrer; Preferably, the second chamber is provided with a sampling port, and a dissolved oxygen detection component is arranged in the sampling port.

5. The columnar algae-bacteria bio-photovoltaic device system according to any one of claims 1 to 4, characterized in that: The columnar algae-bacteria bio-photovoltaic device system further comprises an online collection device, and the online collection device is electrically connected to the first electrode and the second electrode respectively; Preferably, the first electrode and the second electrode independently include any one of graphite, carbon brush, carbon felt, carbon paper and carbon cloth, or a combination of at least two of them.

6. A coupled power generation method of a columnar algae-bacteria bio-photovoltaic device system according to any one of claims 1 to 5, characterized in that: The coupled power generation method comprises: A spacer layer is prepared in advance, the bacterial solution is filled into the second chamber, and the spacer layer is placed above the second chamber so that the hydrogel is in contact with the surface of the bacterial solution; placing a first chamber above the hydrogel, filling the first chamber with algae solution, and electrically connecting the first electrode to the second electrode; Light is provided to promote the algae liquid in the first chamber to photosynthesize, and the generated organic matter passes through the hydrogel into the second chamber, so as to form a voltage between the first electrode and the second electrode.

7. The coupled power generation method according to claim 6, characterized in that: The hydrogel is prepared under a sterile environment; Preferably, the thickness of the hydrogel is 4 to 5 mm.

8. The coupled power generation method according to claim 6 or 7, characterized in that: The bacterial liquid is an electroactive microbial culture liquid; Preferably, the OD of the bacterial solution is 600 0.8~1.0; Preferably, the culture temperature of the electroactive microorganism is 25-30°C; Preferably, the coupled power generation method further comprises: stirring the bacterial liquid in the second chamber; Preferably, the stirring speed is 200-400 rpm.

9. The coupled power generation method according to any one of claims 6 to 8, characterized in that: The OD of the algae solution 730 0.6~0.8; Preferably, the temperature of the algae liquid is 25-30°C; Preferably, the coupled power generation method further comprises: internally circulating the algae liquid in the first chamber; Preferably, the flow rate of the internal circulation is 140-160 mL / min; Preferably, the intensity of the light is 1500-2500Lx.

10. An application of the columnar algae-bacteria bio-photovoltaic device system according to any one of claims 1 to 5, characterized in that: The columnar algae-bacteria biophotovoltaic device system is used to simulate the photoelectric process in the lake ecosystem.

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