Sucrose isomerase mutants and their use in isomaltulose production
CN119432953BActive Publication Date: 2026-08-28JIANGNAN UNIV
Patent Information
- Application Number
- CN202411269891.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-11
- Publication Date
- 2026-08-28
- Estimated Expiration
- 2044-09-11
AI Technical Summary
Technical Problem
[0005]针对上述现有技术中的不足，本发明提供了蔗糖异构酶突变体及其在异麦芽酮糖生产中的应用，目的在于解决现有蔗糖异构酶(SI)酶活力仍较低，导致异麦芽酮糖产量低的技术问题
Benefits of technology
[0035](1)本发明以Serratia plymuthica来源的蔗糖异构酶为亲本酶进行分子改造，对第522位，第222位，第340位或第467位中的至少一个氨基酸进行了突变，获得了一系列酶活提高的突变体。
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Abstract
The application discloses a mutant with improved sucrose isomerase enzyme activity and application thereof, and belongs to the technical field of genetic engineering and enzyme engineering in molecular biology. The known sucrose isomerase parent is subjected to molecular modification by using a site-directed mutagenesis technology, and mutant enzymes T522S / K340D, D222H / K340D, G467N / K340D, T522S, K340D, D222H and G467N are obtained. The catalytic activity of the series of mutant enzymes provided by the application is increased by 10% to 25% compared with that of the original bacteria. The isomaltulose conversion rates of the combined mutant enzymes T522S / K340D, D222H / K340D and G467N / K340D are increased by 2.3%, 1.5% and 1.3% respectively compared with that of the original bacteria, and the combined mutant enzymes have good stability, can be used for biological preparation of isomaltulose, and have potential application prospect.
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Citation Information
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