Potted planting method for striga asiatica

By using specific host plants and preculture techniques in the mixed matrix of soil and sand of the monsoon habitat, the monsoon seed germination and growth of monsoon seeds is solved, and rapid emergence and high-quality seedlings are achieved, which is suitable for commercial applications.

CN119453014AActive Publication Date: 2025-02-18GANNAN MEDICAL UNIV
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Patent Information

Application Number
CN202411603273.5
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-11-11
Publication Date
2025-02-18
Estimated Expiration
2044-11-11

AI Technical Summary

Technical Problem

The prior art is difficult to promote the germination and growth of monolithic seeds quickly and efficiently under non-laboratory conditions, and the existing methods are costly and lack commercial value.

Method used

The mixed matrix of the soil and sand of the monsodium habitat and specific host plants were used, and pre-cultured in clean water and hormone solution containing monsodium cerolide were inoculated around the roots of the host plant for routine watering management.

Benefits of technology

The rapid emergence and early growth activity of single-legged gold seeds has been achieved, shortened the emergence time, improved the quality and health rate of seedlings, and is suitable for commercial applications.

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Abstract

The invention relates to a potting planting method for striga asiatica, which comprises the following steps: pre-culturing mature striga asiatica seeds by using clear water and a hormone solution containing striga asiatica lactone with a specific concentration, and limiting the pre-culturing time, so that the germination of the striga asiatica seeds in soil and the activity improvement in an early growth stage can be effectively promoted; the seedling emergence time of striga asiatica in a soil environment is greatly shortened, and rapid seedling emergence of striga asiatica in soil culture is achieved. And based on the selection of the matrix and the host plant in the method, the drought tolerance and the disease and pest resistance of the striga asiatica seeds are optimized, the resistance of the striga asiatica seeds to the environmental pressure is improved, and the relatively high seedling quality is finally obtained.
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Description

Technical Field

[0001] The present application relates to the technical field of plant cultivation, and in particular to a potted planting method of Striga. Background Art

[0002] The herb is a species of the Scrophulariaceae family. It is an annual semi-parasitic herb that often parasitizes the roots of plants in the Poaceae family. It is mostly found in barren mountains, valleys, grasslands, and fields. It is mainly distributed in Guangdong, Fujian, Jiangxi, Guangxi, Yunnan, and Guizhou in my country. It is also known as malnutrition grass, dwarf, stomata, and rice worm. The dried whole herb is used as medicine. The herb is called stomata, which has the effects of strengthening the spleen and eliminating accumulation, clearing away heat and killing insects. It is a traditional folk medicinal plant in southern my country. In Guangdong and Guangxi, it is often used by the people to treat children's food accumulation and liver fire, with good results. Clinically, it is mainly used to treat children's malnutrition, food injury, jaundice hepatitis, anorexia, etc. Modern studies have shown that it also has the potential for antioxidant, antibacterial, and immune regulation. Due to the unique semi-parasitic characteristics of stomata, the seeds have deep dormancy characteristics. Under natural conditions, the germination rate is 0 in the absence of a host. Under natural conditions, stomata seeds need to be stimulated by host secretions in a specific environment before they can germinate. Even after the existing technology is used to stimulate the germination of seeds, the Striga still needs to find a suitable host and establish a parasitic relationship with it. It can absorb nutrients from the host to maintain the energy required for its own growth and development, germinate, grow seedlings, bloom, and bear fruit to complete its life history. Due to these characteristics, the competitiveness of the Striga community is weak and the natural renewal is slow. In recent years, the market demand for Striga has increased day by day. Excessive excavation and environmental damage have caused the wild resources of Striga to be on the verge of extinction. Therefore, a method of artificial cultivation has emerged. The first step to achieve artificial cultivation is to break the dormancy of Striga seeds and achieve a high germination rate.

[0003] However, the existing methods of artificially cultivating Striga are basically carried out under laboratory conditions. Although they can promote a higher germination rate of Striga, they have strict requirements on the laboratory culture environment. They need to be cultured in MS culture medium and undergo strict aseptic treatment and management. The cost is very expensive and has no practical commercial value. The few soil cultivation methods for Striga currently have too long germination time, low seedling health rate, and low survival rate. Summary of the invention

[0004] Based on this, it is necessary to provide a potted planting method for Strela tatarinowii in response to the above-mentioned technical problems, which can plant Strela tatarinowii in soil in a flowerpot, achieve rapid germination of Strela tatarinowii, and obtain high-quality Strela tatarinowii plants in a short period of time.

[0005] In order to achieve the above objectives, the embodiments of the present invention adopt the following technical solutions:

[0006] A potted planting method of Striga, such as Figure 1 As shown, including:

[0007] Step A: Select a substrate and a host plant, and cultivate the host plant in the substrate until it grows to 10-15 cm; the host plant includes corn, crabgrass, and goosegrass; the substrate is a mixture of soil (clay) in the habitat of Striga and sand, or a mixture of nutrient soil and sand, or a mixture of nutrient soil, sand and perlite;

[0008] Step B: Take mature Striga seeds, place them in clean water and culture them for 3-8 days, take them out and place them in hormone solution and culture them for 3-8 days; mature seeds are full in shape and have brown-black seed coat; the hormone solution is 10 -8 ~10 -3 mol / L strigolactone solution;

[0009] Step C: Take out the Striga seeds and wash them with sterile water, and inoculate the washed Striga seeds around the root system of the host plant;

[0010] Step D: Carry out regular watering management for the Striga seeds.

[0011] Preferably, the combination of the matrix includes:

[0012] A 1:1 mixed matrix of soil (clay) and sand in the habitat of Striga, or a 1:1 mixed matrix of nutrient soil and sand, or a 1:1:1 mixed matrix of nutrient soil, sand and perlite; the composition ratio of the nutrient soil is: 45% coconut bran, 25% peat, 5% corn cob residue, 5% xylose residue, 5% wood ash, 5% bran, 3% Bacillus subtilis, 3% humic acid, and 4% perlite.

[0013] Preferably, the seeds of Striga are cultured in clean water for 3-8 days in a dark environment at a water temperature of 30-32 degrees Celsius; after being taken out, the seeds of Striga are cultured in a hormone solution for 3-8 days in a dark environment at a hormone solution temperature of 32-35 degrees Celsius.

[0014] Preferably, before soaking the Striga seeds in the hormone solution, the Striga seeds are first disinfected with a 3% sodium hypochlorite solution for 3 minutes.

[0015] Strigolactone is a sesquiterpene lactone compound extracted from the Strigol plant. The hormone solution containing Strigolactone can regulate the division, elongation and other physiological functions of seed cells. -8 ~10 -3mol / L is the optimal concentration obtained by our team after many experiments, which can effectively promote seed germination and subsequent seedling growth without causing negative effects caused by drug overdose. The solution temperature is set at 34°C, which is suitable for the chemical activity of strigolactones, while providing a warm environment for seed germination, further activating the growth process inside the seeds, and further promoting the germination and seedling growth of strigolactone seeds in the soil environment.

[0016] The above technical solution has the following advantages and beneficial effects:

[0017] Using clean water and a hormone solution containing strigolactones at a specific concentration, pre-cultivating mature strigospermum seeds in sequence, and limiting the pre-cultivation time, can effectively promote the germination of strigospermum seeds in the soil and enhance their activity in the early growth stage, greatly shortening the emergence time of strigosperm in the soil environment, and achieving rapid emergence of strigosperm in soil culture. The selection of substrates and host plants based on this method optimizes the drought resistance and disease and pest resistance of strigospermum seeds, improves their resistance to environmental pressure, and ultimately achieves a higher seedling quality. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a flow chart of a potted planting method of Striga;

[0019] Figure 2 This is a schematic diagram of the Striga potted plants in Example 1 after 19 days of planting;

[0020] Figure 3 This is a schematic diagram of the Striga potted plants in Example 1 after 33 days of planting;

[0021] Figure 4 This is a schematic diagram of the Striga potted plants in Example 1 after 40 days of planting;

[0022] Figure 5 This is a schematic diagram of the Striga potted plants in Example 2 after 20 days of planting;

[0023] Figure 6 This is a schematic diagram of the Striga potted plants in Example 2 after 30 days of planting;

[0024] Figure 7 This is a schematic diagram of the Striga potted plants in Example 2 after 38 days of planting;

[0025] Figure 8 This is a schematic diagram of the Striga potted plants in Example 3 after 16 days of planting;

[0026] Fig. 9 This is a schematic diagram of the Striga potted plants in Example 3 after 35 days of planting;

[0027] Fig.10This is a schematic diagram of the Striga potted plants in Example 3 after 43 days of planting; DETAILED DESCRIPTION

[0028] In order to make the purpose, technical solution and advantages of the present application more clearly understood, the present application is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present application and are not used to limit the present application.

[0029] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art to which this application belongs. The terms used herein in the specification of this application are only for the purpose of describing specific embodiments and are not intended to limit this application.

[0030] In addition, the technical solutions between the various embodiments of the present invention can be combined with each other, but it must be based on the fact that ordinary technicians in the field can implement it. When the combination of technical solutions is mutually contradictory or cannot be implemented, it should be deemed that such combination of technical solutions does not exist and is not within the scope of protection required by the present invention.

[0031] Example 1

[0032] A potted planting method of Striga is provided, comprising the following steps:

[0033] Step A: Select a 1:1 mixed matrix of soil (clay) and sand in the habitat of Striga, select corn and crabgrass as host plants, and cultivate corn and crabgrass in the soil (clay) matrix in the habitat of Striga to grow to 14 cm;

[0034] Step B: Take mature Striga seeds, place them in clean water for 5 days, the culture environment is a dark environment, and the water temperature is 30 degrees Celsius; after taking them out, place the Striga seeds in a hormone solution for 5 days, the culture environment is a dark environment, and the temperature of the hormone solution is 34 degrees Celsius; mature seeds are full in shape and have brown-black seed coats; the hormone solution is 5*10 -6 mol / L strigolactone solution;

[0035] Step C: Take out the Striga seeds and wash them with sterile water, and inoculate the washed Striga seeds around the root system of the host plant;

[0036] Step D: Carry out regular watering management for the Striga seeds.

[0037] It is understandable that the soil (clay) in the habitat of Striga has fine particles (usually less than 0.002 mm in diameter), high water retention and strong nutrient enrichment ability, and low air permeability. Clay soil can provide long-term moisture and rich nutrients to the roots of Striga through its high water retention and good cation exchange capacity, which is conducive to the growth of plants and the accumulation of medicinal ingredients. However, the high viscosity and low air permeability of clay may limit the expansion of the root system and cause water accumulation and root hypoxia. Therefore, during the cultivation process, adding organic matter or sandy soil to improve the air permeability and drainage of clay can further optimize the growth environment of Striga, promote its root development and the improvement of medicinal ingredients, and help improve the germination speed and quality of Striga.

[0038] It is understandable that culturing the seeds of Striga in clean water for 5 days can promote the water absorption process of the seeds, activate the metabolic activities in the embryo, and prepare for germination. During this period, the seeds swell by absorbing water, the seed coat softens, and the enzymatic reaction in the seeds gradually starts, triggering the germination mechanism of the seeds. The 5-day clean water culture period ensures that the seeds absorb enough water and fully activate their biological functions.

[0039] It is understandable that in the early stage of germination of Strigosa seeds, a dark environment helps the Strigosa seeds to enter the germination state smoothly, because Strigosa seeds may inhibit germination under light conditions. The dark environment simulates the soil state under natural conditions, avoids light interference with the regulatory effects of hormones and enzymes during seed germination, and promotes physiological changes inside seeds.

[0040] It can be understood that water temperature has a direct impact on the rate of seed water absorption and germination. 30 degrees Celsius is a suitable temperature for the water absorption and active metabolism of the seeds of the plant. Providing a suitable physiological temperature can accelerate the seeds from a dormant state to an active metabolic stage. This temperature can activate the enzymatic reaction inside the seeds, promote intracellular metabolism, and enhance the germination ability of the seeds.

[0041] It can be understood that strigolactone is a sesquiterpene lactone compound extracted from the strigolactone plant. The hormone solution containing strigolactone can regulate the division, elongation and other physiological functions of seed cells. -6 The concentration of 1.5 mol / L is the optimal concentration obtained by our team after many experiments. It can effectively promote seed germination and subsequent seedling growth without causing negative effects caused by drug overdose. The solution temperature is set at 34°C, which is suitable for the chemical activity of strigolactones and provides a warm environment for seed germination, further activating the growth process inside the seeds and further promoting the germination and seedling growth of strigolactone seeds in the soil environment.

[0042] It can be understood that sterile water is obtained by treating water containing bacterial masses by physical methods to obtain bacteria-free water. It can be obtained by killing and filtering microorganisms in water using steam method, UHT heat method, ultraviolet sterilization method, ozone method and physical filtration method. Using sterile water to wash seeds disinfected with sodium hypochlorite solution or alcohol can wash away the sodium hypochlorite solution or alcohol remaining on the seeds to avoid affecting the embryo.

[0043] A potted planting method of a Striga described in Example 1, a 1:1 mixed matrix of Striga habitat soil (clay) and sand is selected, corn and crabgrass are selected as host plants, mature Striga seeds are first placed in clear water for cultivation in a dark environment, and then placed in a hormone solution containing Striga lactone in a dark environment for cultivation, and the cultivated Striga seeds are cleaned and inoculated around the host plant root system. This method can effectively promote the germination of Striga seeds in the soil and the activity of the early growth stage, so that it can fully absorb water during the cultivation process, greatly shorten the emergence time of Striga in the soil environment, and achieve the rapid emergence of Striga soil culture.

[0044] The Striga spatholobi cultured in Example 1 had a relatively fast emergence, and the seedlings grew robustly, but the number of seedlings was moderate, and the seedlings could be seen breaking through the soil in 17 days. Figure 2 It can be clearly seen that the seedlings have the embryonic form of a Striga plant on day 19. Figure 3 It can be clearly seen that the plants bloomed on day 33. Figure 4 It can be clearly seen that the plant can bear fruit in 40 days and the health of the Strigosa tricholoma plant is good.

[0045] Example 2

[0046] A potted planting method of Striga is provided, comprising the following steps:

[0047] Step A: Select a 1:1 mixed matrix of nutrient soil and sand, the composition ratio of the nutrient soil is: 45% coconut bran, 25% peat, 5% corn cob residue, 5% xylose residue, 5% plant ash, 5% bran, 3% Bacillus subtilis, 3% humic acid, and 4% perlite; select corn, crabgrass and goosegrass as host plants, and cultivate corn, crabgrass and goosegrass in the 1:1 mixed matrix of nutrient soil and sand until they grow to 12 cm;

[0048] Step B: Take mature Striga seeds, place them in clean water for 3 days in a dark environment with a water temperature of 32 degrees Celsius; take them out and place them in a hormone solution for 6 days in a dark environment with a hormone solution temperature of 35 degrees Celsius; mature seeds are full in shape and have brown-black seed coats; the hormone solution is 5*10 -6 mol / L strigolactone solution;

[0049] Step C: Take out the Striga seeds and wash them with sterile water, and inoculate the washed Striga seeds around the root system of the host plant;

[0050] Step D: Carry out regular watering management for the Striga seeds.

[0051] It can be understood that the nutrient soil used in this embodiment, through the scientific formulation of component proportions, can provide plants with balanced moisture, nutrients and aeration, and is particularly suitable for the cultivation and growth optimization of various crops or medicinal plants; the components and proportions of the nutrient soil in this embodiment are not strictly fixed and can be flexibly adjusted according to needs.

[0052] A potted planting method of Striga described in Example 2 above, selects a 1:1 mixed matrix of nutrient soil and sand, selects corn, crabgrass and goosegrass as host plants, first places mature Striga seeds in clear water for cultivation in a dark environment, then places them in a hormone solution containing Striga lactone in a dark environment for cultivation, and washes the cultivated Striga seeds and inoculates them around the root system of the host plant. This method ensures that the seedling rate of Striga seeds is maximized without destroying the seeds.

[0053] The Striga cultured in Example 2 developed well, and the cultured Striga sprouted quickly and in large numbers. The seedlings could be seen breaking through the soil in 18 days. Figure 5 It can be clearly seen that after 20 days, the Striga plant has taken shape. Figure 6 It can be clearly seen that the plants bloomed in 30 days. Figure 7 It can be clearly seen that the plants will bear fruit in 38 days.

[0054] Example 3

[0055] A potted planting method of Striga is provided, comprising the following steps:

[0056] Step A: Select a 1:1:1 mixed matrix of nutrient soil, sand and perlite, the composition ratio of the nutrient soil is: 45% coconut bran, 25% peat, 5% corn cob residue, 5% xylose residue, 5% plant ash, 5% bran, 3% Bacillus subtilis, 3% humic acid, and 4% perlite; select corn, crabgrass and goosegrass as host plants, and cultivate corn, crabgrass and goosegrass in the soil (clay) matrix of the Striga habitat to grow to 10 cm;

[0057] Step B: Take mature Striga seeds, place them in clean water for 3 days, the culture environment is a dark environment, and the water temperature is 30 degrees Celsius; after taking them out, use 3% sodium hypochlorite solution to disinfect the Striga seeds for 3 minutes; after disinfection, place the Striga seeds in a hormone solution for 3 days, the culture environment is a dark environment, and the temperature of the hormone solution is 33 degrees Celsius; mature seeds are full in shape and have brown-black seed coats; the hormone solution is 5*10 -8 mol / L strigolactone solution;

[0058] Step C: Take out the Striga seeds and wash them with sterile water, and inoculate the washed Striga seeds around the root system of the host plant;

[0059] Step D: Carry out regular watering management for the Striga seeds.

[0060] Example 3 After taking out the Striga seeds from the clean water, the Striga seeds are disinfected with a 3% sodium hypochlorite solution for 3 minutes, and then placed in a hormone solution for cultivation; this method has the advantages of removing pathogens on the surface of seeds, dissolving wax on the surface of seeds, improving germination rate, and preventing mold growth. At the same time, the disinfection process is fast and efficient and does not damage the seeds. This method can greatly improve the robustness of the Striga seedlings and improve the quality of the Striga plants while ensuring the number and speed of seedlings.

[0061] The Striga spatholobi cultured in Example 3 developed well, and the Striga spatholobi seedlings broke through the soil in 14 days. Figure 8 It can be clearly seen that the 16-day-old tricholoma has begun to take shape. Fig. 9 It can be clearly seen that the plants bloomed in 35 days. Fig.10 It can be clearly seen that the plants can bear fruit in 43 days, the number of seedlings is large, and the health of the Striga plants is good.

[0062] Through the above three embodiments, good Striga plants were obtained by potted planting. Embodiments 1, 2, and 3 all placed Striga seeds in clean water and a hormone solution containing Striga lactone for different days, which can effectively promote the germination of Striga seeds in the soil and improve their activity in the early growth stage; Embodiments 2 and 3 both added nutrient soil to the culture matrix to ensure that the seedling rate of Striga seeds is maximized without destroying the seeds; Embodiment 3 used 3% sodium hypochlorite solution to disinfect Striga seeds for 3 minutes and then placed them in a hormone solution for cultivation, which has the advantages of removing pathogens on the surface of the seeds, dissolving the wax layer that affects germination, etc., improving the germination rate, and preventing the growth of mold. At the same time, the disinfection process is fast and efficient and does not damage the seeds.

[0063] The following are some comparative examples for Example 1, Example 2 and Example 3 respectively:

[0064] The pre-cultivation process of the Striga plantation in Example 1 was changed, including the process flow and the concentration of the hormone solution, to form the following three corresponding Comparative Examples 1, 2 and 3:

[0065] Comparative Example 1

[0066] Compared with Example 1, after the Striga seeds were cultured in clean water, they were not cultured in a hormone solution containing strigolactones, as follows:

[0067] Step A: Select a 1:1 mixed matrix of soil (clay) and sand in the habitat of Striga, select corn and crabgrass as host plants, and cultivate corn and crabgrass in the soil (clay) matrix in the habitat of Striga to grow to 15 cm;

[0068] Step B: Take mature Striga seeds, place them in clean water and culture them for 5 days in a dark environment at a water temperature of 30 degrees Celsius; mature seeds are full in shape and have brown-black seed coats;

[0069] Step C: Take out the Striga seeds and wash them with sterile water, and inoculate the washed Striga seeds around the root system of the host plant;

[0070] Step D: Carry out regular watering management for the Striga seeds.

[0071] Comparative Example 1: After the seeds of Striga were cultured in clean water, they were not cultured in a hormone solution containing strigolactone. After 50 days, the Striga seedlings broke through the soil, and the development rate of the cultivated Striga was extremely low. This shows that the strigolactone solution plays an important role in breaking the dormancy of Striga seeds and promoting their germination.

[0072] Comparative Example 2

[0073] Compared with Example 1, the seeds of Striga were cultured in clear water and then placed in 5*10 -2 The culture was carried out in a 0.1 mol / L trichololactone solution, and the other processes were the same.

[0074] Comparative Example 2 uses 5*10 -2 mol / L of strigolactone solution was used as a hormone solution to pre-cultivate the strigolactone seeds. No strigolactone seedlings broke through the soil after 60 days. This shows that the high concentration of strigolactone solution has had a destructive effect on the strigolactone seeds, causing the seeds to become necrotic and unable to germinate.

[0075] Comparative Example 3

[0076] Compared with Example 1, the seeds of Strigosa Striga are not cultured in clean water, but are directly cultured in a hormone solution containing Strigosa lactones, and the other processes are the same.

[0077] In comparative example 3, the seeds of Strigosa argyi were not cultured in clean water, but directly placed in a hormone solution containing Strigosa lactone for culture. It took 27 days for the Strigosa argyi seedlings to break through the soil. This shows that clean water plays a key role in activating seeds, stimulating metabolic activities in the seed embryo, and preparing for germination.

[0078] It can be seen from Example 1 and Comparative Examples 1, 2 and 3 that, compared with culturing the seeds of Strigosa chinensis in clean water without continuing to culture them in a hormone solution containing Strigosa lactone, the method adopted in Example 1 greatly shortens the germination speed of the seeds of Strigosa chinensis, and a large number of seedlings emerge. The seedlings can be seen breaking through the soil in 17 days, the seedlings can be seen to have the embryonic form of Strigosa chinensis plants in 19 days, the plants can be seen to bloom in 33 days, and the plants can be seen to bear fruit in 40 days. The Strigosa chinensis plants are in good health.

[0079] By changing the components and proportions of the matrix in Example 2, the following corresponding Comparative Examples 4 and 5 are formed:

[0080] Comparative Example 4

[0081] Compared with Example 2, a 1:1 mixed matrix of ordinary soil and sand was used to plant the host plant, and the other processes were the same.

[0082] The matrix used in Comparative Example 4 is a mixture of ordinary soil and sand, and the nutrient soil in the embodiment is not used in the matrix. The host plant cultured in this way grows slowly. Compared with Example 2, it takes an additional 1-2 weeks for the host plant to grow to 12 cm; and the seedling speed of the tricholoma seeds is also slow. The seedlings can be seen breaking through the soil in 24 days, the plants can be seen blooming in 40 days, and the plants can be seen bearing fruit in 48 days. The number of seedlings is also significantly reduced.

[0083] Comparative Example 5

[0084] Compared with Example 2, a 4:1 mixed matrix of nutrient soil and sand was used to plant host plants, and other processes were the same.

[0085] In Comparative Example 5, a 4:1 mixed matrix of nutrient soil and sand was used to plant the host plant. The seedling rate of the seeds of the single-legged gold was reduced and the seedling speed was also slow. This was because the soil contained less sand than in Example 2, which resulted in reduced air permeability of the matrix, causing oxygen to be unable to effectively reach the roots of the seeds, and the seeds were unable to germinate smoothly.

[0086] It can be seen from Example 2 and Comparative Examples 4 and 5 that selecting a 1:1 mixed matrix of nutrient soil and sand in a strict ratio can maximize the seedling rate of the Striga seeds without damaging the seeds.

[0087] The concentration and type of disinfectant, as well as the lighting conditions and temperature conditions in Example 3 were changed respectively to form the following corresponding Comparative Examples 6, 7 and 8:

[0088] Comparative Example 6

[0089] Compared with Example 3, the seeds of Striga were disinfected with 10% sodium hypochlorite solution, and the other processes were the same.

[0090] Comparative Example 6 used 10% sodium hypochlorite solution to disinfect the Striga seeds, but no Striga seedlings emerged after 50 days. This shows that the high concentration of sodium hypochlorite solution has destroyed the Striga seeds, causing the seeds to become necrotic and unable to germinate.

[0091] Comparative Example 7

[0092] Compared with Example 3, 3% hydrogen peroxide solution was used to disinfect the Striga seeds, and the other processes were the same.

[0093] In Comparative Example 7, 3% hydrogen peroxide solution was used to disinfect the Striga seeds, but the Striga seeds did not germinate. This shows that 3% hydrogen peroxide solution can inhibit the germination of the Striga seeds or have a destructive effect on the Striga seeds.

[0094] Comparative Example 8

[0095] Compared with Example 3, Comparative Example 8 uses different lighting environments and different temperatures to pre-treat the Striga seeds, and the other steps are the same. The specific treatment effects are as shown in the following table:

[0096] Table 1: Days for germination of Striga in different culture environments

[0097]

[0098]

[0099] Comparative Example 8 In the comparative example, different light environments and different temperatures were used to cultivate the seeds of the Striga. The number of days for the Striga seedlings to emerge from the soil varied greatly.

[0100] Compared with Comparative Example 8, Example 3 uses a dark environment culture in clear water with a water temperature of 30 degrees Celsius, and a dark environment culture in a hormone solution with a hormone solution temperature of 33 degrees Celsius; the effect is far superior to other solutions.

[0101] The technical features of the above embodiments may be combined arbitrarily. To make the description concise, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0102] The above-mentioned embodiments only express several implementation methods of the present application, and the description thereof is relatively detailed, but it cannot be understood as limiting the scope of the invention patent. It should be pointed out that, for a person of ordinary skill in the art, several variations and improvements can be made without departing from the concept of the present application, and these all belong to the protection scope of the present application. Therefore, the protection scope of the patent of the present application shall be subject to the attached claims.

Claims

1. A potted planting method of Striga, characterized in that: The method comprises: Step A: selecting a matrix and a host plant, and cultivating the host plant in the matrix until it grows to 10-15 cm; the host plant includes corn, crabgrass, and goosegrass; the matrix is ​​a mixture of soil (clay) in the habitat of Striga and sand, or a mixture of nutrient soil and sand, or a mixture of nutrient soil, sand and perlite; Step B: Take mature Striga seeds, place them in clean water and culture them for 3-8 days, take them out and place them in a hormone solution and culture them for 3-8 days; the mature seeds are full-bodied and have brown-black seed coats; the hormone solution is 10 -8 ~10 -3 mol / L strigolactone solution; Step C: taking out the Striga seeds and washing them with sterile water, and inoculating the washed Striga seeds around the root system of the host plant; Step D: performing conventional watering management on the Striga seeds.

2. The potted planting method of Striga according to claim 1, characterized in that: The combination of the matrix includes: A 1:1 mixed matrix of soil (clay) and sand in the habitat of Striga, or a 1:1 mixed matrix of nutrient soil and sand, or a 1:1:1 mixed matrix of nutrient soil, sand and perlite; the composition ratio of the nutrient soil is: 45% coconut bran, 25% peat, 5% corn cob residue, 5% xylose residue, 5% wood ash, 5% bran, 3% Bacillus subtilis, 3% humic acid, and 4% perlite.

3. The potted planting method of Striga according to claim 1 or 2, characterized in that: The seeds of Striga are placed in clean water and cultured for 3-8 days in a dark environment at a water temperature of 30-32 degrees Celsius; After being taken out, the tricholoma seeds are placed in a hormone solution and cultured for 3-8 days in a dark environment. The temperature of the hormone solution is 32-35 degrees Celsius.

4. The potted planting method of Striga according to claim 1 or 2, characterized in that: Before soaking the Striga seeds in the hormone solution, the Striga seeds are first disinfected with a 3% sodium hypochlorite solution for 3 minutes.

Citation Information

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