Application of oxocyclopentanone as nitrogen fertilizer stabilizer to source reduction and sink increase in soil

Abstract

The invention discloses application of oxocyclopentanone as a nitrogen fertilizer stabilizer to source reduction and sink increase in soil, and belongs to the technical field of novel inhibitor materials, the source reduction is that oxocyclopentanone is applied to the soil to inhibit N2O and N2 emission reduction; and the increase and sink is to promote the reduction of N2O to N2. The cyclopentanone serving as a stabilizer is applied to soil, so that the activity of denitrification-related functional genes (nirS, nirK and nosZ) can be adjusted, the denitrification process in the soil is adjusted, and the nitrogen loss of a denitrification path is reduced. When cyclopentanone is applied to soil, expression of denitrification related enzymes such as nitrite reductase and nitrous oxide reductase can be adjusted by adjusting related functional genes. Cyclopentanone can reduce accumulated release of N2O and N2 in soil, emission reduction of N2O is achieved, gaseous nitrogen loss of farmland soil is reduced, and the nitrogen utilization rate of the soil is increased. Cyclopentanone can effectively delay the denitrification rate in soil, reduce the loss of gaseous nitrogen, improve the nitrogen utilization rate and further increase the crop yield.

Description

Technical Field

[0001] The invention belongs to the technical field of novel inhibitor materials, and in particular relates to the application of oxocyclopentanone as a nitrogen fertilizer stabilizer in soil to reduce sources and increase sinks. Background Art

[0002] In agricultural production, nitrogen fertilizer is an important factor affecting plant growth and yield, and it also determines the quality of agricultural products, playing an irreplaceable role. When nitrogen fertilizer is applied to the soil, in addition to the part absorbed and utilized by plants, it will be lost through various channels, so that the utilization rate of nitrogen fertilizer is only in the range of 30-50%. Among them, the denitrification process is one of the important ways for farmland nitrogen loss. The addition of nitrogen fertilizer promotes the denitrification process in farmland soil, and farmland nitrogen loss occurs. N2 and N2O are important ways for nitrogen loss during soil denitrification. In farmland, the denitrification process contributes more than 60% to N2 emissions, and it is also the most important gaseous nitrogen loss process in the natural environment.

[0003] At present, nitrogen loss can be reduced and the nitrogen fertilizer utilization rate of farmland soil can be improved through fertilization management, application of inhibitors, soil improvement and other methods. Among them, the use of inhibitors is one of the most economical and effective means. Inhibitors widely used in agricultural production include urease inhibitors and nitrification inhibitors, which delay the nitrogen conversion process in the soil by regulating the urea hydrolysis and ammonia oxidation processes respectively. Delaying the denitrification process in farmland by applying inhibitors is of great significance to improving the utilization rate of nitrogen fertilizer and rationally regulating the application of nitrogen fertilizer in farmland. Summary of the invention

[0004] In view of this, the object of the present invention is to provide the effect of oxocyclopentanone as a nitrogen fertilizer stabilizer in inhibiting the denitrification process.

[0005] In order to achieve the above object, the present invention provides the following technical solutions:

[0006] The invention discloses an application of oxocyclopentanone as a nitrogen fertilizer stabilizer in soil to reduce sources and increase sinks. The oxocyclopentanone is applied to the soil to inhibit N2O emission reduction to achieve source reduction; and promote the reduction of N2O to N2 to reduce N2O emission to achieve sink increase.

[0007] Furthermore, the emission reduction of N2O is 63.20-77.89%, and the emission reduction of N2 is 5-48%.

[0008] Furthermore, the oxocyclopentanone and nitrogen fertilizer are mixed and applied to the soil.

[0009] Furthermore, the amount of oxocyclopentanone applied is 1% of the amount of nitrogen fertilizer applied.

[0010] The beneficial effects of the present invention are:

[0011] 1. Compared with traditional inhibitors, the oxocyclopentanone used as a nitrogen fertilizer stabilizer of the present invention is derived from plant materials and has the characteristics of low cost, environmental friendliness, and synergistic effect. At the same time, due to the complexity of its components, it has more possibilities for nitrogen conversion in the soil compared with traditional inhibitors.

[0012] 2. The oxocyclopentanone of the present invention can be applied in the soil as a stabilizer to regulate the activity of denitrification-related functional genes (nirS, nirK, nosZ), regulate the denitrification process in the soil, and reduce the nitrogen loss of the denitrification pathway. Oxocyclopentanone can be applied in the soil to regulate the expression of denitrification-related enzymes such as nitrite reductase and nitrous oxide reductase by regulating the relevant functional genes. Oxocyclopentanone can reduce the cumulative release of N2O and N2 in the soil, realize N2O emission reduction, reduce the gaseous nitrogen loss of farmland soil, and improve the nitrogen utilization rate of the soil. Oxocyclopentanone can effectively delay the denitrification rate in the soil, reduce the gaseous nitrogen loss, improve the nitrogen utilization rate, and then increase crop yield.

[0013] 3. In the present invention, oxocyclopentanone can effectively delay the conversion process of urea in the soil and increase NH4 + -N availability in soil.

[0014] Other advantages, objectives and features of the present invention will be described in the following description and will be apparent to those skilled in the art to some extent, or those skilled in the art may be taught from the practice of the present invention. The objectives and other advantages of the present invention may be realized and obtained through the following description. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to make the purpose, technical solution and beneficial effects of the invention clearer, the present invention provides the following drawings for illustration:

[0016] Figure 1 The change of inorganic nitrogen in the soil during the cultivation process of the present invention;

[0017] Figure 2 The emission changes of N2O and N2 during the cultivation process of the present invention;

[0018] Figure 3 The changes of denitrification functional genes in the cultivation process of the present invention;

[0019] Figure 4 This is a graph showing the proportion of N2O in soil nitrogen-containing gas emissions after CCO is applied in the present invention;

[0020] Figure 5 This is a demonstration diagram of N2O emission reduction achieved by reducing the release of N2O under field conditions under the CCO of the present invention. DETAILED DESCRIPTION

[0021] Definitions of various abbreviations in the present invention: 1. CCO: oxocyclopentanone; 2. CK: blank control; 3. U: single application of urea; 4. U+CCO: combined application of urea and oxocyclopentanone.

[0022] Design of culture experiment:

[0023] 1. Select two test soils: Jiangxi red soil and Gansu desert irrigated soil;

[0024] 2. Three treatments were carried out on Jiangxi red soil and Gansu desert irrigation soil: blank control (CK), single application of urea (U), and combination of urea and cyclopentanone (U+CCO).

[0025] The test results of Jiangxi red soil and Gansu desert soil after treatment are as follows:

[0026] from Figure 1 It was found that during the cultivation process, stable isotope tracing technology and fluorescence quantitative PCR technology were used to determine the changes in inorganic nitrogen in the two soils, the emissions of N2O and N2, and the abundance of denitrification-related functional genes nirS, nirK, and nosZ.

[0027] 3. Compare the changes of relevant indicators in the two soils and reveal the effects of cyclopentanone on the denitrification process in the soil:

[0028] from Figure 2 It can be seen that urea combined with cyclopentanone can prolong the + -N effective time in the soil and reduce the emission of N2O and N2.

[0029] from Figure 3 It was found that the abundance of the denitrification functional gene nirS decreased in red soil, the nirK gene was inhibited in desert irrigation soil, and nosZ had no significant change.

[0030] from Figure 4 It can be seen that the application of CCO can change the emission ratio of N2O and N2, and reduce the proportion of N2O in soil nitrogen gas emissions.

[0031] from Figure 5 It can be seen that CCO can reduce the release of N2O under field conditions and achieve N2O emission reduction.

[0032] Finally, it should be noted that the above preferred embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. Although the present invention has been described in detail through the above preferred embodiments, those skilled in the art should understand that various changes can be made in form and details without departing from the scope defined by the claims of the present invention.

Claims

1. The application of oxocyclopentanone as a nitrogen fertilizer stabilizer in soil to reduce sources and increase sinks is characterized by: The oxocyclopentanone is applied to the soil to inhibit the reduction of N2O emissions to achieve source reduction; and promote the reduction of N2O to N2 to reduce the emission of N2O to achieve sink enhancement.

2. The application of oxocyclopentanone as a nitrogen fertilizer stabilizer in soil to reduce sources and increase sinks is characterized by: The N2O emission reduction is 63.20-77.89%, and the N2 reduction is 5-48%.

3. The application of oxocyclopentanone as a nitrogen fertilizer stabilizer in soil to reduce sources and increase sinks is characterized by: The oxocyclopentanone and nitrogen fertilizer are mixed and applied to the soil.

4. The application of oxocyclopentanone as a nitrogen fertilizer stabilizer in soil to reduce sources and increase sinks is characterized by: The amount of oxocyclopentanone applied is 1% of the amount of nitrogen fertilizer applied.

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