Preparation method of functional wormwood composite essential oil

Abstract

The invention relates to the technical field of essential oil, and discloses a preparation method of functional wormwood composite essential oil. The functional wormwood compound essential oil is prepared from the following raw materials in parts by volume: 20 to 30 parts of wormwood essential oil, 5 to 10 parts of cassia twig essential oil, 3 to 5 parts of herba schizonepetae essential oil, 1 to 3 parts of herba lycopodii essential oil and 1 to 3 parts of rhizoma corydalis essential oil. According to the functional wormwood composite essential oil provided by the invention, the DPPH free radical scavenging rate can reach 98.1%, the ABTS < + > free radical scavenging rate can reach 96.9%, and the functional wormwood composite essential oil has relatively high antioxidant activity.

Description

Technical Field

[0001] The invention relates to the technical field of essential oils, and in particular to a method for preparing functional wormwood composite essential oil. Background Art

[0002] Modern theories of aging are represented by the free radical theory, the biological clock theory of aging, the gene regulation disorder theory, the cell apoptosis theory, the immune dysfunction theory, etc. Among them, the free radical theory is one of the most representative theories of aging. The free radical theory believes that free radicals are intermediate metabolites of various biochemical reactions in human life activities and have high chemical activity. If free radicals are produced too much and cannot be cleared in time, they will attack the body's macromolecules and various organelles, causing various damages at the molecular, cellular and tissue and organ levels, accelerating the body's aging process and inducing various diseases.

[0003] With the continuous development of social and economic levels, people pay more and more attention to healthy living, and delaying aging has become a hot topic of concern. Substances with antioxidant effects can directly react with free radicals and convert them into stable compounds, thereby delaying aging. Based on this, functional health products with antioxidant activity have been favored and sought after by consumers.

[0004] Modern medical research shows that mugwort is rich in volatile oils and flavonoid active ingredients and has a strong antioxidant effect. Therefore, providing a mugwort compound essential oil with antioxidant activity has become a technical problem that needs to be urgently solved by those skilled in the art. Summary of the invention

[0005] The purpose of the invention is to provide a method for preparing a functional wormwood compound essential oil, wherein the functional wormwood compound essential oil has high antioxidant activity.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] One of the technical solutions of the present invention:

[0008] A functional wormwood compound essential oil comprises the following raw materials in parts by volume:

[0009] 20-30 parts of wormwood essential oil, 5-10 parts of cassia twig essential oil, 3-5 parts of schizonepeta essential oil, 1-3 parts of lycopodiella cernua essential oil and 1-3 parts of corydalis yanhusuo essential oil.

[0010] Furthermore, the preparation method of the wormwood essential oil comprises the following steps: placing the wormwood in an ethanol solvent, soaking, and refluxing to obtain the wormwood essential oil.

[0011] Furthermore, the ethanol solvent is an ethanol solvent with a volume fraction of 30% to 40%.

[0012] Furthermore, the solid-liquid ratio of the wormwood to the ethanol solvent is 1 g: (5-15) mL.

[0013] Furthermore, the soaking is soaking at 30° C. for 40 minutes.

[0014] Furthermore, the reflux is performed at 60-80° C. for 4-6 hours.

[0015] Furthermore, the preparation method of the cassia twig essential oil comprises the following steps: placing cassia twig in water, soaking, and distilling to obtain the cassia twig essential oil.

[0016] Furthermore, the material-liquid ratio of the cassia twig to water is 1g:10mL.

[0017] Furthermore, the soaking is performed at room temperature for 30 minutes.

[0018] Furthermore, the distillation is carried out at 110° C. for 40 minutes.

[0019] Furthermore, the preparation method of the schizonepeta essential oil comprises the following steps: placing schizonepeta in an ethanol solvent, soaking, and refluxing to obtain the schizonepeta essential oil.

[0020] Furthermore, the ethanol solvent is an ethanol solvent with a volume fraction of 60%.

[0021] Furthermore, the solid-liquid ratio of the Nepeta tenuifolia to the ethanol solvent is 1 g:20 mL.

[0022] Furthermore, the soaking is performed at room temperature for 10 minutes.

[0023] Furthermore, the reflux is reflux at 50° C. for 1.5 h.

[0024] Furthermore, the preparation method of the lycopodiella ciliata essential oil comprises the following steps: placing the lycopodiella ciliata in an ethanol solvent, soaking, and refluxing to obtain the lycopodiella ciliata essential oil.

[0025] Furthermore, the ethanol solvent is an ethanol solvent with a volume fraction of 20%.

[0026] Furthermore, the solid-liquid ratio of the Nepeta tenuifolia to the ethanol solvent is 1 g:10 mL.

[0027] Furthermore, the soaking is performed at room temperature for 30 minutes.

[0028] Furthermore, the reflux is reflux at 80° C. for 1 h.

[0029] Furthermore, the preparation method of the Corydalis yanhusuo essential oil comprises the following steps: placing Corydalis yanhusuo in water, soaking, and distilling to obtain the Corydalis yanhusuo essential oil.

[0030] Furthermore, the liquid ratio of cassia twig to water is 1g:20mL.

[0031] Furthermore, the soaking is performed at room temperature for 15 minutes.

[0032] Furthermore, the distillation is carried out at 75° C. for 20 min.

[0033] The second technical solution of the present invention:

[0034] The preparation method of the functional wormwood compound essential oil comprises the following steps:

[0035] The functional wormwood composite essential oil is obtained by mixing wormwood essential oil, cassia twig essential oil, schizonepeta essential oil, lycopodiella ciliata essential oil and corydalis yanhusuo essential oil.

[0036] Furthermore, the mixing is carried out at 4°C.

[0037] The third technical solution of the present invention:

[0038] The application of the above functional wormwood compound essential oil in delaying aging.

[0039] Compared with the prior art, the present invention has the following beneficial effects:

[0040] The functional wormwood composite essential oil provided by the invention has a DPPH free radical scavenging rate of up to 98.1%, an ABTS+ free radical scavenging rate of up to 96.9%, and has high antioxidant activity. DETAILED DESCRIPTION

[0041] Now, various exemplary embodiments of the present invention are described in detail, and this detailed description should not be considered as a limitation of the present invention, but should be understood as a more detailed description of certain aspects, characteristics and embodiments of the present invention. It should be understood that the terms described in the present invention are only for describing specific embodiments and are not used to limit the present invention.

[0042] In addition, for the numerical range in the present invention, it is understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Each smaller range between the intermediate value in any stated value or stated range and any other stated value or intermediate value in the range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0043] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0044] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to the skilled artisan. The present invention description and examples are exemplary only.

[0045] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0046] In the following examples, the room temperature is 25±2°C.

[0047] In the following examples, the wormwood, cinnamon twig, schizonepeta, lycopodiella cuneata and corydalis yanhusuo were all purchased from commercial suppliers, as shown in Table 1.

[0048] Table 1 Raw materials

[0049]

[0050] In the following embodiment, a method for preparing a functional wormwood composite essential oil comprises the following steps:

[0051] 1) placing the wormwood in an ethanol solvent with a volume fraction of 30% to 40% according to a solid-liquid ratio of wormwood to 30% to 40% ethanol solvent of 1 g: (5 to 15) mL, soaking the wormwood in an ethanol solvent with a volume fraction of 30% to 40% at 30° C. for 40 min, then refluxing at 60 to 80° C. for 4 to 6 h, filtering and removing impurities, and obtaining wormwood essential oil;

[0052] 2) placing the cinnamon twig in water at a material-liquid ratio of 1 g to 10 mL, soaking the cinnamon twig in water at room temperature for 30 min, and then distilling at 110° C. for 40 min to separate the oil and water, thereby obtaining cinnamon twig essential oil;

[0053] 3) placing Nepeta in 60% by volume ethanol solvent at a solid-liquid ratio of 1 g to 20 mL, soaking at room temperature for 10 min, then refluxing at 50° C. for 1.5 h, filtering and removing impurities, and obtaining Nepeta essential oil;

[0054] 4) placing the herb in a 20% by volume ethanol solvent at a solid-liquid ratio of 1 g to 10 mL, soaking the herb at room temperature for 30 min, then refluxing at 80° C. for 1 h, filtering and removing impurities, and obtaining herb essential oil;

[0055] 5) placing the Corydalis yanhusuo in water at a solid-liquid ratio of 1 g to 20 mL, soaking the Corydalis yanhusuo in water at room temperature for 15 min, and then distilling the water at 75° C. for 20 min to separate the oil from the water, thereby obtaining Corydalis yanhusuo essential oil;

[0056] 6) Weigh each raw material according to the following volume parts:

[0057] 20-30 parts of wormwood essential oil, 5-10 parts of cassia twig essential oil, 3-5 parts of schizonepeta essential oil, 1-3 parts of lycopodiella essential oil and 1-3 parts of corydalis essential oil;

[0058] 7) The raw materials weighed in step 6) are mixed at 4° C. to obtain the functional wormwood composite essential oil.

[0059] Example 1

[0060] A functional wormwood compound essential oil

[0061] 1) placing the wormwood in a 30% by volume ethanol solvent at a solid-liquid ratio of 1 g to 5 mL, soaking the wormwood in a 30% by volume ethanol solvent at 30° C. for 40 min, then refluxing at 60° C. for 4 h, filtering and removing impurities, and obtaining wormwood essential oil;

[0062] 2) placing the cinnamon twig in water at a material-liquid ratio of 1 g to 10 mL, soaking the cinnamon twig in water at room temperature for 30 min, and then distilling at 110° C. for 40 min to separate the oil and water, thereby obtaining cinnamon twig essential oil;

[0063] 3) placing Nepeta in 60% by volume ethanol solvent at a solid-liquid ratio of 1 g to 20 mL, soaking at room temperature for 10 min, then refluxing at 50° C. for 1.5 h, filtering and removing impurities, and obtaining Nepeta essential oil;

[0064] 4) placing the herb in a 20% by volume ethanol solvent at a solid-liquid ratio of 1 g to 10 mL, soaking the herb at room temperature for 30 min, then refluxing at 80° C. for 1 h, filtering and removing impurities, and obtaining herb essential oil;

[0065] 5) placing the Corydalis yanhusuo in water at a solid-liquid ratio of 1 g to 20 mL, soaking the Corydalis yanhusuo in water at room temperature for 15 min, and then distilling the water at 75° C. for 20 min to separate the oil from the water, thereby obtaining Corydalis yanhusuo essential oil;

[0066] 6) Weigh each raw material according to the following volume parts:

[0067] 20 parts of Artemisia argyi essential oil, 5 parts of cassia twig essential oil, 3 parts of Nepeta tenuifolia essential oil, 1 part of Lycopodiella cernua essential oil and 1 part of Corydalis yanhusuo essential oil;

[0068] 7) The raw materials weighed in step 6) are mixed at 4° C. to obtain the functional wormwood composite essential oil.

[0069] Example 2

[0070] A functional wormwood compound essential oil

[0071] 1) placing the wormwood in a 30% by volume ethanol solvent at a solid-liquid ratio of 1 g to 5 mL, soaking the wormwood in a 30% by volume ethanol solvent at 30° C. for 40 min, then refluxing at 60° C. for 4 h, filtering and removing impurities, and obtaining wormwood essential oil;

[0072] 2) placing the cinnamon twig in water at a material-liquid ratio of 1 g to 10 mL, soaking the cinnamon twig in water at room temperature for 30 min, and then distilling at 110° C. for 40 min to separate the oil and water, thereby obtaining cinnamon twig essential oil;

[0073] 3) placing Nepeta in 60% by volume ethanol solvent at a solid-liquid ratio of 1 g to 20 mL, soaking at room temperature for 10 min, then refluxing at 50° C. for 1.5 h, filtering and removing impurities, and obtaining Nepeta essential oil;

[0074] 4) placing the herb in a 20% by volume ethanol solvent at a solid-liquid ratio of 1 g to 10 mL, soaking the herb at room temperature for 30 min, then refluxing at 80° C. for 1 h, filtering and removing impurities, and obtaining herb essential oil;

[0075] 5) placing the Corydalis yanhusuo in water at a solid-liquid ratio of 1 g to 20 mL, soaking the Corydalis yanhusuo in water at room temperature for 15 min, and then distilling the water at 75° C. for 20 min to separate the oil from the water, thereby obtaining Corydalis yanhusuo essential oil;

[0076] 6) Weigh each raw material according to the following volume parts:

[0077] 25 parts of Artemisia argyi essential oil, 8 parts of cassia twig essential oil, 4 parts of Nepeta tenuifolia essential oil, 2 parts of Lycopodiella ciliata essential oil and 2 parts of Corydalis yanhusuo essential oil;

[0078] 7) The raw materials weighed in step 6) are mixed at 4° C. to obtain the functional wormwood composite essential oil.

[0079] Example 3

[0080] A functional wormwood compound essential oil

[0081] 1) placing the wormwood in a 30% by volume ethanol solvent at a solid-liquid ratio of 1 g to 5 mL, soaking the wormwood in a 30% by volume ethanol solvent at 30° C. for 40 min, then refluxing at 60° C. for 4 h, filtering and removing impurities, and obtaining wormwood essential oil;

[0082] 2) placing the cinnamon twig in water at a material-liquid ratio of 1 g to 10 mL, soaking the cinnamon twig in water at room temperature for 30 min, and then distilling at 110° C. for 40 min to separate the oil and water, thereby obtaining cinnamon twig essential oil;

[0083] 3) placing Nepeta in 60% by volume ethanol solvent at a solid-liquid ratio of 1 g to 20 mL, soaking at room temperature for 10 min, then refluxing at 50° C. for 1.5 h, filtering and removing impurities, and obtaining Nepeta essential oil;

[0084] 4) placing the herb in a 20% by volume ethanol solvent at a solid-liquid ratio of 1 g to 10 mL, soaking the herb at room temperature for 30 min, then refluxing at 80° C. for 1 h, filtering and removing impurities, and obtaining herb essential oil;

[0085] 5) placing the Corydalis yanhusuo in water at a solid-liquid ratio of 1 g to 20 mL, soaking the Corydalis yanhusuo in water at room temperature for 15 min, and then distilling the water at 75° C. for 20 min to separate the oil from the water, thereby obtaining Corydalis yanhusuo essential oil;

[0086] 6) Weigh each raw material according to the following volume parts:

[0087] 30 parts of Artemisia argyi essential oil, 10 parts of cassia twig essential oil, 5 parts of Nepeta tenuifolia essential oil, 3 parts of Lycopodiella cernua essential oil and 3 parts of Corydalis yanhusuo essential oil;

[0088] 7) The raw materials weighed in step 6) are mixed at 4° C. to obtain the functional wormwood composite essential oil.

[0089] Comparative Example 1

[0090] A functional wormwood compound essential oil

[0091] 1) placing the wormwood in a 30% by volume ethanol solvent at a solid-liquid ratio of 1 g to 5 mL, soaking the wormwood in a 30% by volume ethanol solvent at 30° C. for 40 min, then refluxing at 60° C. for 4 h, filtering and removing impurities, and obtaining wormwood essential oil;

[0092] 2) placing Nepeta in 60% by volume ethanol solvent at a solid-liquid ratio of 1 g to 20 mL, soaking at room temperature for 10 min, then refluxing at 50° C. for 1.5 h, filtering and removing impurities, and obtaining Nepeta essential oil;

[0093] 3) placing the herb in a 20% by volume ethanol solvent at a solid-liquid ratio of 1 g to 10 mL, soaking the herb at room temperature for 30 min, then refluxing at 80° C. for 1 h, filtering and removing impurities, and obtaining herb essential oil;

[0094] 4) placing the Corydalis yanhusuo in water at a solid-liquid ratio of 1 g to 20 mL, soaking the Corydalis yanhusuo in water at room temperature for 15 min, and then distilling at 75° C. for 20 min to separate the oil and water, thereby obtaining Corydalis yanhusuo essential oil;

[0095] 5) Weigh each raw material according to the following volume parts:

[0096] 25 parts of wormwood essential oil, 4 parts of schizonepeta essential oil, 2 parts of lycopodiella essential oil and 2 parts of corydalis essential oil;

[0097] 6) The raw materials weighed in step 5) are mixed at 4° C. to obtain the functional wormwood composite essential oil.

[0098] Comparative Example 2

[0099] A functional wormwood compound essential oil

[0100] 1) placing the wormwood in a 30% by volume ethanol solvent at a solid-liquid ratio of 1 g to 5 mL, soaking the wormwood in a 30% by volume ethanol solvent at 30° C. for 40 min, then refluxing at 60° C. for 4 h, filtering and removing impurities, and obtaining wormwood essential oil;

[0101] 2) placing the cinnamon twig in water at a material-liquid ratio of 1 g to 10 mL, soaking the cinnamon twig in water at room temperature for 30 min, and then distilling at 110° C. for 40 min to separate the oil and water, thereby obtaining cinnamon twig essential oil;

[0102] 3) placing the herb in a 20% by volume ethanol solvent at a solid-liquid ratio of 1 g to 10 mL, soaking the herb at room temperature for 30 min, then refluxing at 80° C. for 1 h, filtering and removing impurities, and obtaining herb essential oil;

[0103] 4) placing the Corydalis yanhusuo in water at a solid-liquid ratio of 1 g to 20 mL, soaking the Corydalis yanhusuo in water at room temperature for 15 min, and then distilling the water at 75° C. for 20 min to separate the oil from the water, thereby obtaining Corydalis yanhusuo essential oil;

[0104] 5) Weigh each raw material according to the following volume parts:

[0105] 25 parts of Artemisia argyi essential oil, 8 parts of cassia twig essential oil, 2 parts of Lycopodiella ciliata essential oil and 2 parts of Corydalis yanhusuo essential oil;

[0106] 6) The raw materials weighed in step 5) are mixed at 4° C. to obtain the functional wormwood composite essential oil.

[0107] Comparative Example 3

[0108] A functional wormwood compound essential oil

[0109] 1) placing the wormwood in a 30% by volume ethanol solvent at a solid-liquid ratio of 1 g to 5 mL, soaking the wormwood in a 30% by volume ethanol solvent at 30° C. for 40 min, then refluxing at 60° C. for 4 h, filtering and removing impurities, and obtaining wormwood essential oil;

[0110] 2) placing the cinnamon twig in water at a material-liquid ratio of 1 g to 10 mL, soaking the cinnamon twig in water at room temperature for 30 min, and then distilling at 110° C. for 40 min to separate the oil and water, thereby obtaining cinnamon twig essential oil;

[0111] 3) placing Nepeta in 60% by volume ethanol solvent at a solid-liquid ratio of 1 g to 20 mL, soaking at room temperature for 10 min, then refluxing at 50° C. for 1.5 h, filtering and removing impurities, and obtaining Nepeta essential oil;

[0112] 4) placing the Corydalis yanhusuo in water at a solid-liquid ratio of 1 g to 20 mL, soaking the Corydalis yanhusuo in water at room temperature for 15 min, and then distilling the water at 75° C. for 20 min to separate the oil from the water, thereby obtaining Corydalis yanhusuo essential oil;

[0113] 5) Weigh each raw material according to the following volume parts:

[0114] 25 parts of wormwood essential oil, 8 parts of cassia twig essential oil, 4 parts of schizonepeta essential oil and 2 parts of corydalis essential oil;

[0115] 6) The raw materials weighed in step 5) are mixed at 4° C. to obtain the functional wormwood composite essential oil.

[0116] Comparative Example 4

[0117] A functional wormwood compound essential oil

[0118] 1) placing the wormwood in a 30% by volume ethanol solvent at a solid-liquid ratio of 1 g to 5 mL, soaking the wormwood in a 30% by volume ethanol solvent at 30° C. for 40 min, then refluxing at 60° C. for 4 h, filtering and removing impurities, and obtaining wormwood essential oil;

[0119] 2) placing the cinnamon twig in water at a material-liquid ratio of 1 g to 10 mL, soaking the cinnamon twig in water at room temperature for 30 min, and then distilling at 110° C. for 40 min to separate the oil and water, thereby obtaining cinnamon twig essential oil;

[0120] 3) placing Nepeta in 60% by volume ethanol solvent at a solid-liquid ratio of 1 g to 20 mL, soaking at room temperature for 10 min, then refluxing at 50° C. for 1.5 h, filtering and removing impurities, and obtaining Nepeta essential oil;

[0121] 4) placing the herb in a 20% by volume ethanol solvent at a solid-liquid ratio of 1 g to 10 mL, soaking the herb at room temperature for 30 min, then refluxing at 80° C. for 1 h, filtering and removing impurities, and obtaining herb essential oil;

[0122] 5) Weigh each raw material according to the following volume parts:

[0123] 25 parts of wormwood essential oil, 8 parts of cassia twig essential oil, 4 parts of schizonepeta essential oil and 2 parts of lycopodiella ciliata essential oil;

[0124] 6) The raw materials weighed in step 5) are mixed at 4° C. to obtain the functional wormwood composite essential oil.

[0125] The functional wormwood composite essential oils prepared in Examples 1 to 3 and Comparative Examples 1 to 4 were tested for antioxidant activity, including a DPPH free radical capture ability experiment and an ABTS+ free radical scavenging experiment. The results of the antioxidant activity test are shown in Table 2.

[0126] Table 2 Antioxidant activity test results

[0127]

[0128] It can be seen from the data in Table 2 that the DPPH radical scavenging rate of the functional wormwood composite essential oil provided by the present invention can reach 93.3%, and the ABTS+ radical scavenging rate can reach 92.1%, and it has high antioxidant activity; and omitting any one of the essential oils in the raw materials will lead to a decrease in the DPPH radical scavenging rate and the ABTS+ radical scavenging rate of the composite essential oil, which indicates that the raw materials provided by the present invention have a synergistic effect.

[0129] Example 4

[0130] Optimization of the preparation process of wormwood essential oil

[0131] 1. Optimization of ethanol solvent volume fraction

[0132] According to the solid-liquid ratio of wormwood to ethanol solvent with a volume fraction of 30%, 32%, 34%, 36%, 38% or 40% being 1g:5mL, wormwood is placed in an ethanol solvent, soaked at 30°C for 20 minutes, then refluxed at 60°C for 4 hours, filtered and impurities removed, to obtain the wormwood essential oil.

[0133] The prepared wormwood essential oil was used as a raw material, and a functional wormwood composite essential oil was prepared with reference to Example 2, and the prepared functional wormwood composite essential oil was tested for antioxidant activity. The results of the antioxidant activity test are shown in Table 3;

[0134] Table 3 Antioxidant activity test results

[0135]

[0136]

[0137] It can be seen from the data in Table 3 that when the wormwood essential oil is extracted with 38% ethanol solvent by volume as the extractant, the functional wormwood composite essential oil can achieve the highest DPPH free radical scavenging rate, so the volume fraction of the ethanol solvent is preferably 38%.

[0138] 2. Optimization of material-liquid ratio

[0139] According to the solid-liquid ratio of mugwort to 38% ethanol solvent of 1g:5mL, 1g:7mL, 1g:9mL, 1g:11mL, 1g:13mL or 1g:15mL, the mugwort is placed in the ethanol solvent, soaked at 30°C for 40min, then refluxed at 60°C for 4h, filtered and impurities removed, to obtain the mugwort essential oil.

[0140] The prepared wormwood essential oil was used as a raw material, and a functional wormwood composite essential oil was prepared with reference to Example 2, and the prepared functional wormwood composite essential oil was tested for antioxidant activity. The results of the antioxidant activity test are shown in Table 4;

[0141] Table 4 Antioxidant activity test results

[0142]

[0143] It can be seen from the data in Table 4 that the functional wormwood compound essential oil can achieve the highest DPPH radical scavenging rate by controlling the solid-liquid ratio to 1 g:9 mL for extracting wormwood essential oil, so the solid-liquid ratio is preferably 1 g:9 mL.

[0144] 3. Optimization of reflux temperature

[0145] According to the solid-liquid ratio of mugwort to 38% ethanol solvent by volume of 1g:9mL, the mugwort is placed in the ethanol solvent, soaked at 30°C for 40min, then refluxed at 60°C, 65°C, 70°C, 75°C or 80°C for 4h, filtered and impurities removed, to obtain the mugwort essential oil.

[0146] The prepared wormwood essential oil was used as a raw material, and a functional wormwood composite essential oil was prepared with reference to Example 2, and the prepared functional wormwood composite essential oil was tested for antioxidant activity. The results of the antioxidant activity test are shown in Table 5;

[0147] Table 5 Antioxidant activity test results

[0148]

[0149] It can be seen from the data in Table 5 that when the reflux temperature is controlled at 65°C to extract the wormwood essential oil, the functional wormwood composite essential oil can achieve the highest DPPH radical scavenging rate, so the reflux temperature is preferably 65°C.

[0150] 4. Optimization of reflow time

[0151] According to the solid-liquid ratio of wormwood to 38% ethanol solvent by volume of 1g:9mL, wormwood is placed in ethanol solvent, soaked at 30°C for 40min, then refluxed at 65°C for 4h, 4.5h, 5h, 5.5h, and 6h, filtered to remove impurities, and obtain the wormwood essential oil.

[0152] The above-prepared wormwood essential oil was used as a raw material, and a functional wormwood composite essential oil was prepared with reference to Example 2, and the prepared functional wormwood composite essential oil was tested for antioxidant activity. The results of the antioxidant activity test are shown in Table 6;

[0153] Table 6 Antioxidant activity test results

[0154]

[0155] It can be seen from the data in Table 6 that the reflux time is controlled to be 5.5 h for the extraction of wormwood essential oil. The functional wormwood composite essential oil can achieve the highest DPPH free radical scavenging rate, so the reflux time is preferably 5.5 h.

[0156] The wormwood essential oil was prepared by the optimal wormwood essential oil preparation process obtained by the above screening, and the functional wormwood composite essential oil was prepared with reference to Example 2, and the antioxidant activity of the prepared functional wormwood composite essential oil was tested. After testing, the DPPH free radical scavenging rate of the functional wormwood composite essential oil could reach 98.1%, and the ABTS+ free radical scavenging rate could reach 96.9%, which had high antioxidant activity.

[0157] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the above embodiments, ordinary technicians in the relevant field should understand that the specific implementation methods of the present invention can still be modified or replaced by equivalents. Any modification or equivalent replacement that does not depart from the spirit and scope of the present invention should be covered within the scope of protection of the claims of the present invention.

Claims

1. A functional wormwood composite essential oil, characterized in that: The following ingredients are included in volume: 20-30 parts of wormwood essential oil, 5-10 parts of cassia twig essential oil, 3-5 parts of schizonepeta essential oil, 1-3 parts of lycopodiella cernua essential oil and 1-3 parts of corydalis yanhusuo essential oil.

2. A functional wormwood composite essential oil according to claim 1, characterized in that: The preparation method of the wormwood essential oil comprises the following steps: placing the wormwood in an ethanol solvent, soaking, and refluxing to obtain the wormwood essential oil.

3. A functional wormwood composite essential oil according to claim 2, characterized in that, The ethanol solvent is an ethanol solvent with a volume fraction of 30% to 40%; the solid-liquid ratio of the wormwood to the ethanol solvent is 1g: (5 to 15) mL; the soaking is soaking at 30°C for 40 minutes; and the reflux is reflux at 60 to 80°C for 4 to 6 hours.

4. A functional wormwood composite essential oil according to claim 1, characterized in that: The preparation method of the cassia twig essential oil comprises the following steps: placing cassia twigs in water, soaking, and distilling to obtain the cassia twig essential oil.

5. A functional wormwood composite essential oil according to claim 1, characterized in that: The preparation method of the schizonepeta essential oil comprises the following steps: placing schizonepeta in an ethanol solvent, soaking, and refluxing to obtain the schizonepeta essential oil.

6. A functional wormwood composite essential oil according to claim 1, characterized in that: The preparation method of the lycopodiella ciliata essential oil comprises the following steps: placing the lycopodiella ciliata in an ethanol solvent, soaking it, and refluxing it to obtain the lycopodiella ciliata essential oil.

7. A functional wormwood composite essential oil according to claim 1, characterized in that: The preparation method of the Corydalis yanhusuo essential oil comprises the following steps: placing the Corydalis yanhusuo in water, soaking it, and distilling it to obtain the Corydalis yanhusuo essential oil.

8. A method for preparing the functional wormwood compound essential oil according to any one of claims 1 to 7, characterized in that: The following steps are involved: The functional wormwood composite essential oil is obtained by mixing wormwood essential oil, cassia twig essential oil, schizonepeta essential oil, lycopodiella ciliata essential oil and corydalis yanhusuo essential oil.

9. The preparation method according to claim 8, characterized in that: The mixing was carried out at 4°C.

10. Use of the functional wormwood compound essential oil according to any one of claims 1 to 7 in delaying aging.

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