A system and method for analyzing the neurotoxicity of cyanobacteria toxins to aquatic organisms

Abstract

The present invention discloses a system and method for analyzing the neurotoxicity of cyanobacteria toxins to aquatic organisms. The system comprises a base plate, an electric guide rail mechanism disposed on the base plate, a slider of the electric guide rail mechanism being provided with a container-carrying mechanism, a first liquid-feeding mechanism and a second liquid-feeding mechanism, both disposed on the base plate, and a fully automated fluorescent stereo microscope disposed on the base plate and downstream of the second liquid-feeding mechanism, an image acquisition module being provided on the eyepiece of the fully automated fluorescent stereo microscope, and the image acquisition module being communicatively connected to an analysis module. The present invention utilizes the first liquid-feeding mechanism to inject CYN solutions of varying concentrations into a reservoir, and the second liquid-feeding mechanism to inject a fixative solution into the reservoir. Subsequently, the fully automated fluorescent stereo microscope, the image acquisition module, and the analysis module analyze the effects of the various CYN solutions on aquatic organisms, thereby facilitating ease of use and reducing labor intensity.

Description

Technical Field

[0001] The present invention relates to the technical field of aquatic organism research, and in particular to a system and method for analyzing the neurotoxicity of cyanobacterial toxins to aquatic organisms. Background Art

[0002] In recent years, due to changes in the natural environment and increased human activities, the occurrence of cyanobacteria in aquatic environments has increased significantly. Some species of these cyanobacteria can produce harmful metabolites, namely cyanotoxins, including MCs, STXs, ANA-a, BMAA, and CYN. CYN can cause nervous system disorders and even death in aquatic organisms, posing a significant threat to the aquatic environment.

[0003] Currently, the analysis of the neurotoxicity of cyanobacterial toxins to aquatic organisms is typically performed manually through a series of tedious steps, including injecting CYN solution into the sample and transferring the sample to designated equipment for analysis and evaluation. This is cumbersome, increases labor intensity, and reduces work efficiency, hindering large-scale and efficient neurotoxicity analysis of cyanobacterial toxins. To this end, we propose a system and method for analyzing the neurotoxicity of cyanobacterial toxins to aquatic organisms. Summary of the Invention

[0004] The object of the present invention is to provide a system and method for analyzing the neurotoxicity of cyanobacterial toxins to aquatic organisms, so as to solve the problems raised in the above-mentioned background technology.

[0005] To achieve the above object, the present invention provides the following technical solutions:

[0006] A neurotoxicity analysis system for cyanobacteria toxins to aquatic organisms, comprising a base plate and:

[0007] An electric guide rail mechanism is provided on the bottom plate and extends along the length of the bottom plate. A carrying container mechanism is provided on a slider of the electric guide rail mechanism. The carrying container mechanism includes a plate body having a plurality of placement slots for placing aquatic organisms.

[0008] A first liquid supply mechanism and a second liquid supply mechanism are both provided on the bottom plate, the first liquid supply mechanism being used to supply CYN solutions of different concentrations into the plurality of placement tanks, respectively, and the second liquid supply mechanism being used to supply a fixative solution for fixing aquatic organisms into the placement tanks; and

[0009] A fully automatic fluorescent stereo microscope is arranged on the bottom plate and located downstream of the second liquid supply mechanism. The eyepiece of the fully automatic fluorescent stereo microscope is provided with an image acquisition module for collecting fluorescent image data of aquatic organisms. The image acquisition module is communicatively connected to an analysis module. The analysis module is used to receive the fluorescent image data and compare it with the fluorescent image data of normal aquatic organisms in a database to obtain an analysis result.

[0010] A further improvement is that the analysis system further includes:

[0011] An incubator is provided on the bottom plate and between the first liquid supply mechanism and the second liquid supply mechanism, both sides of the incubator are hollow, and the incubator is sleeved on the outside of the electric guide rail mechanism; and a closing mechanism, the closing mechanism comprising:

[0012] The two groups of door bodies are slidably arranged on both sides of the incubator and are used to close both sides of the incubator. The tops of the two groups of door bodies are commonly connected to a bracket, and the bracket is connected to the top of the incubator through a second telescopic device.

[0013] A further improvement is that the placement slots are arranged in three rows and three columns, the bottom of the base plate is provided with a box body with a hollow top, the bottom of the box body is slidingly provided with a connecting block detachably connected to the slider of the electric guide rail mechanism, the slider and one end of the bottom of the box body are connected by a telescopic device, and the telescopic device is used to drive the box body to move along its width direction relative to the connecting block, the bottom of the placement slot is provided with several groups of microholes connected to the box body, the bottom of the base plate and the position inside the box body is provided with a movable baffle, a through opening is provided on the movable baffle at a position staggered from the placement slot, the movable baffle is connected to the box body by a spring, an electromagnetic block is embedded in the movable baffle, and the bottom of the plate body is embedded with a magnetic block adsorbed and connected to the energized electromagnetic block.

[0014] A further improvement is that the first liquid supply mechanism and the second liquid supply mechanism have the same structure, and the first liquid supply mechanism includes:

[0015] The carrier is arranged on the bottom plate. The carrier is provided with a plurality of groups of placement cylinders for storing liquids. The number of the placement cylinders corresponds to the number of a row of placement slots. A liquid outlet pipe is provided at the bottom of the placement cylinder, and a solenoid valve is provided on the liquid outlet pipe.

[0016] A further improvement is that the side wall of the box body is provided with a side panel extending in its horizontal direction, and a plurality of groups of arc-shaped protrusions are integrated on the side panel, each of the arc-shaped protrusions corresponds to a row of placement slots, and the side wall of the support frame is vertically movable and provided with a contact rod for slidingly abutting against the top of the side panel and the arc-shaped protrusions, and the outer wall of the contact rod is provided with an elastic member for driving it to reset downward, and the top of the contact rod is provided with a conductive sheet 2 electrically connected to an external power supply, and the side wall of the support frame is provided with a conductive sheet 1 electrically connected to the solenoid valve, and when the conductive sheet 1 and the conductive sheet 2 are in contact, the bottom of the contact rod contacts the highest point of the top of an arc-shaped protrusion, and the liquid outlet pipe of the placement cylinder corresponds to a row of placement slots.

[0017] A further improvement is that a detection sensor is provided on the bottom plate and between the incubator and the second liquid supply mechanism. The detection sensor is electrically connected to the controller and is used to send a signal to the controller when detecting that the carrying container mechanism has reached a preset position, so that the controller controls the electromagnetic block to cut off power.

[0018] A further improvement is that the base plate and the box body are detachably connected.

[0019] A further improvement is that a slot is provided on the top of the slider of the electric guide rail mechanism, which is adapted to the connecting block, a card rod is movably inserted into the side wall of the slider of the electric guide rail mechanism, and a slot for the card block to enter is provided on the outer wall of the connecting block, an operating block is provided at the outer end of the card rod, and a spring is provided on the outer wall of the card rod for driving the card rod to reset.

[0020] A method for analyzing the neurotoxicity of cyanobacteria toxins to aquatic organisms, using the above-mentioned analysis system, comprises the following steps:

[0021] S1: Select several transgenic zebrafish with specific neural markers and place them in different placement tanks;

[0022] S2: driving the carrying container mechanism to the first liquid applying mechanism via the electric guide mechanism, sequentially injecting CYN solutions of different concentrations into different placement tanks via the first liquid applying mechanism, after the transgenic zebrafish are exposed to the CYN solution for a preset time, driving the carrying container mechanism to the second liquid applying mechanism via the electric guide mechanism, injecting fixative solution into different placement tanks via the second liquid applying mechanism to fix the transgenic zebrafish in the placement tanks, then driving the carrying container mechanism to the fully automatic fluorescent stereo microscope via the electric guide mechanism, acquiring fluorescent image data of the transgenic zebrafish in the placement tanks via the fully automatic fluorescent stereo microscope and the image acquisition module, and sending the fluorescent image data to the analysis module;

[0023] S3: The analysis module is used to receive the fluorescence image data and compare it with the fluorescence image data of normal aquatic organisms in the database, so as to obtain the effects of different concentrations of CYN solutions on transgenic zebrafish.

[0024] Compared with the prior art, the present invention has the following beneficial effects:

[0025] The present invention can transport a carrying container mechanism containing aquatic organisms to a first liquid supply mechanism and a second liquid supply mechanism through an electric guide rail mechanism. The first liquid supply mechanism automatically injects CYN solutions of different concentrations into different placement slots of the carrying container mechanism. The second liquid supply mechanism automatically injects a fixative solution into different placement slots of the carrying container mechanism to fix the aquatic organisms. The electric guide rail mechanism then transports the carrying container mechanism to a fully automatic fluorescent stereo microscope. The fully automatic fluorescent stereo microscope, image acquisition module, and analysis module are used to analyze and obtain results of the effects of CYN solutions of different concentrations on the aquatic organisms. The invention is easy to use, reduces manual labor intensity, improves work efficiency, and facilitates large-scale and efficient neurotoxicity analysis of cyanobacterial toxins. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 Schematic diagram of the neurotoxicity analysis system of the present invention;

[0027] Figure 2 For the present invention Figure 1 Another perspective structural diagram;

[0028] Figure 3 A top view of the structure of the carrier container of the present invention;

[0029] Figure 4 A side view of the structure of the carrier container of the present invention;

[0030] Figure 5 It is a schematic diagram of the partial structure of the carrying container mechanism of the present invention;

[0031] Figure 6 It is a structural schematic diagram of the first liquid supply mechanism of the present invention.

[0032] In the figure: 100, bottom plate; 101, detection sensor; 200, electric guide rail mechanism; 300, carrying container mechanism; 301, plate body; 302, placement groove; 303, micropore; 304, box body; 305, connecting block; 306, telescopic device 1; 307, operating block; 308, side plate; 309, arc-shaped protrusion; 310, movable baffle; 311, electromagnetic block; 400, incubator; 500, first liquid feeding mechanism; 501, carrier frame; 502, placement cylinder; 503, liquid outlet pipe; 504, electromagnetic valve; 505, contact rod; 506, elastic member; 507, conductive sheet 1; 600, second liquid feeding mechanism; 700, fully automatic fluorescent microscope; 800, image acquisition module; 900, closing mechanism; 901, door body; 902, telescopic device 2. DETAILED DESCRIPTION

[0033] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0034] A system for analyzing neurotoxicity of cyanobacteria toxins to aquatic organisms includes a base plate 100 and further includes:

[0035] The electric guide rail mechanism 200 is provided on the base plate 100 and extends along the length of the base plate 100. The electric guide rail mechanism 200 is a conventional structure in the art, including a guide rail, a slider, and a motor, and will not be described in detail here. The slider of the electric guide rail mechanism 200 is provided with a carrying container mechanism 300. The carrying container mechanism 300 includes a plate body 301, and the plate body 301 is provided with a plurality of placement slots 302 for placing aquatic organisms. The placed aquatic organisms can preferably be transgenic zebrafish strains with specific neural markers: Tg(huc:eGFP), Tg(gad1b:mCherry), and Tg(hb9:eGFP);

[0036] The first liquid feeding mechanism 500 and the second liquid feeding mechanism 600 are both disposed on the bottom plate 100. The first liquid feeding mechanism 500 is used to supply CYN solutions of different concentrations into the plurality of placement tanks 302. Three concentrations of 20, 200, and 2000 nM (or 9, 90, and 900 μg / L) can be set. The second liquid feeding mechanism 600 is used to supply a fixative solution for fixing aquatic organisms into the placement tanks 302. The fixative solution may be methylcellulose.

[0037] The fully automatic fluorescent stereo microscope 700 is arranged on the bottom plate 100 and is located downstream of the second liquid supply mechanism 600. The eyepiece of the fully automatic fluorescent stereo microscope 700 is provided with an image acquisition module 800 for collecting fluorescent image data of aquatic organisms. The image acquisition module 800 can be a camera, etc. The image acquisition module 800 is connected to the analysis module in communication. The analysis module is used to receive the fluorescent image data and compare it with the fluorescent image data of normal aquatic organisms in the database to obtain the analysis results. The fluorescent images of the central nervous system and motor nerves of the aquatic organisms are captured by the fully automatic fluorescent stereo microscope 700. The optical image analysis module evaluates the specific effects of CYN on the early development of the zebrafish nervous system by quantitatively analyzing the expression of fluorescent proteins (including measuring the expression level of fluorescent proteins (reflected by fluorescence intensity) and the length of synapses). The analysis module can be, for example, a computer device. For example, by comparing the changes in fluorescence intensity in aquatic organisms under different concentrations of CYN solutions, the effects of CYN solutions on the development of the zebrafish nervous system can be evaluated; by measuring the lengths of synapses in aquatic organisms under different concentrations of CYN solutions, the effects of CYN on the development of the zebrafish nervous system can be further understood.

[0038] Preferably, the analysis system of this embodiment further includes:

[0039] The incubator 400 is disposed on the base plate 100 and located between the first liquid supply mechanism 500 and the second liquid supply mechanism 600. The incubator 400 is conventional and will not be described in detail herein. Both sides of the incubator 400 are hollow. The incubator 400 is mounted on the outside of the electric guide rail mechanism 200. After the CYN solution is injected into the placement tank 302, the carrying container mechanism 300 can be placed into the incubator 400 via the electric guide rail mechanism 200. During the time that the aquatic organisms are exposed to the CYN solution, the temperature of the incubator 400 can be controlled at 28±1 degrees Celsius. The photoperiod is set to 14 hours of light and 10 hours of dark, for an exposure time of 3-6 days. Furthermore, the sealing mechanism 900 includes:

[0040] Two sets of door bodies 901 are respectively slidably arranged on both sides of the incubator 400 and are used to close both sides of the incubator 400. The tops of the two sets of door bodies 901 are commonly connected to a bracket, and the bracket is connected to the top of the incubator 400 through a telescopic device 902. The telescopic device 902 is, for example, an electric telescopic rod. The two sets of door bodies 901 can be driven to open or close through the telescopic device 902, so that the carrying container mechanism 300 can enter and exit the incubator 400 or the aquatic organisms in the carrying container mechanism 300 can be stably exposed in the incubator 400.

[0041] Preferably, the placement slots 302 of this embodiment are arranged in three rows and three columns. When in use, the concentration of CYN solution in the placement slots 302 in each row is the same, and three CYN solutions of different concentrations are respectively put into the three placement slots 302 in a column. The bottom of the bottom plate 100 is provided with a box body 304 with a hollow top. The bottom of the box body 304 is provided with a connecting block 305 that is detachably connected to the slider of the electric guide rail mechanism 200. The slider and the bottom end of the box body 304 are connected by a telescopic device 306. The telescopic device 306 is, for example, an electric telescopic rod. The telescopic device 306 is used to drive the box body 304 to move relative to the connecting block 305 along its width direction. When the bottom plate 100 is under the fully automatic fluorescent microscope 700, the placement slots 302 at different positions on the bottom plate 100 can be made to correspond to the detection end of the fully automatic fluorescent microscope 700 by operating the electric guide rail mechanism 200 or the telescopic device 306. The bottom of the placement slot 302 is provided with a Several groups of micropores 303 connected to the box body 304 are used to discharge the CYN solution in the placement tank 302, but the aquatic organisms in the placement tank 302 will not be discharged from the micropores 303. A movable baffle 310 is provided at the bottom of the bottom plate 100 and in the position inside the box body 304. A through hole is opened on the movable baffle 310 at a position offset from the placement tank 302. The movable baffle 310 is connected to the box body 304 by a spring. An electromagnetic block 311 is embedded in the movable baffle 310. 1 is embedded with a magnetic block that is adsorbed and connected to an energized electromagnetic block 311. By disconnecting the power of the electromagnetic block 311, the movable baffle 310 moves downward under the action of the spring, and the CYN solution in the placement tank 302 is discharged into the box body 304 through the micropores 303, so that a fixing solution can be subsequently injected into the placement tank 302 to fix the aquatic organisms in the placement tank 302. It should be noted that when the fixing solution is injected into the placement tank 302, the electromagnetic block 311 is in an energized state.

[0042] Preferably, the first liquid applying mechanism 500 and the second liquid applying mechanism 600 of this embodiment have the same structure. The first liquid applying mechanism 500 includes:

[0043] The carrier 501 is provided on the base plate 100 and is provided with several groups of placement tubes 502 for storing liquids. The number of placement tubes 502 corresponds one-to-one to the number of placement slots 302 in a row, that is, there are three groups of placement tubes 502. The three groups of placement tubes 502 of the first liquid feeding mechanism 500 sequentially store CYN solutions of different concentrations, and the three groups of placement tubes 502 of the second liquid feeding mechanism 600 store fixative solutions. A liquid outlet pipe 503 is provided at the bottom of the placement tube 502, and a solenoid valve 504 is provided on the liquid outlet pipe 503. By opening the solenoid valve 504, the solution in the placement tube 502 can be discharged from the liquid outlet pipe 503.

[0044] As a preferred embodiment, the side wall of the box body 304 of this embodiment is provided with a side panel 308 extending in its horizontal direction, and a plurality of groups of arc-shaped protrusions 309 are integrated on the side panel 308, each arc-shaped protrusion 309 corresponds to a row of placement slots 302, and the side wall of the carrier 501 is vertically movable and provided with a contact rod 505 for sliding against the top of the side panel 308 and the arc-shaped protrusion 309. The bottom end of the contact rod 505 can be embedded with a ball to contact the side panel 308 and the arc-shaped protrusion 309, and the outer wall of the contact rod 505 is provided with an elastic member 506 for driving it to reset downward, for example, one end of the elastic member 506 is in contact with the contact rod The outer wall of 505 is connected, and the other end is connected to the outer wall of the support frame 501. The top of the contact rod 505 is provided with a conductive sheet 2 electrically connected to the external power supply, and the side wall of the support frame 501 is provided with a conductive sheet 1 507 electrically connected to the solenoid valve 504. When the conductive sheet 1 507 and the conductive sheet 2 are in contact, the bottom of the contact rod 505 contacts the highest point of the top of an arc-shaped protrusion 309, and the liquid outlet pipe 503 of the placement cylinder 502 corresponds to a row of placement grooves 302, so that the solution in the placement cylinder 502 accurately enters the placement groove 302. When the conductive sheet 1 507 and the conductive sheet 2 are separated, the liquid outlet pipe 503 stops discharging liquid.

[0045] Preferably, a detection sensor 101 is provided on the bottom plate 100 of this embodiment and located between the incubator 400 and the second liquid feeding mechanism 600. The detection sensor 101 is, for example, an infrared sensor, a position sensor, etc. The detection sensor 101 is electrically connected to the controller and is used to send a signal to the controller when detecting that the carrying container mechanism 300 has reached a preset position, so that the controller controls the electromagnetic block 311 to cut off the power. It should be noted that when the carrying container mechanism 300 moves to the second liquid feeding mechanism 600, the detection sensor 101 does not detect the carrying container mechanism 300, and the controller controls the electromagnetic block 311 to be energized.

[0046] Preferably, the bottom plate 100 and the box body 304 of this embodiment are detachably connected, for example, by bolts or snaps.

[0047] Preferably, a slot matching the connecting block 305 is provided on the top of the slider of the electric guide rail mechanism 200 of this embodiment, a card rod is movably inserted into the side wall of the slider of the electric guide rail mechanism 200, and a slot for the card block to enter is provided on the outer wall of the connecting block 305, an operating block 307 is provided on the outer end of the card rod, and a spring for driving the card rod to reset is provided on the outer wall of the card rod, one end of the spring is connected to the side wall of the slider, and the other end is connected to the operating block 307.

[0048] A method for analyzing the neurotoxicity of cyanobacteria toxins to aquatic organisms, using the above-mentioned analysis system, comprises the following steps:

[0049] S1: Select several transgenic zebrafish with specific neural markers, such as 9 each of the strains Tg(huc:eGFP), Tg(gad1b:mCherry), and Tg(hb9:eGFP), and place them in different placement tanks 302. Specifically, transgenic zebrafish of the same strain are placed in the placement tanks 302 in the same row;

[0050] S2: The electric guide rail mechanism 200 drives the carrier container mechanism 300 to the first liquid feeding mechanism 500. The first liquid feeding mechanism 500 sequentially injects CYN solutions of different concentrations, namely 20, 200 and 2000 nM (or 9, 90 and 900 μg / L), into different placement tanks 302. After the transgenic zebrafish are exposed to the CYN solution for a preset time, i.e., 3-6 days, the electric guide rail mechanism 200 drives the carrier container mechanism 300 to the second liquid feeding mechanism 600. A fixative solution, namely, 2.5% methylcellulose, is injected into different placement tanks 302 by the second liquid-loading mechanism 600 to fix the transgenic zebrafish in the placement tanks 302. Subsequently, the electric guide rail mechanism 200 drives the carrying container mechanism 300 to the fully-automatic fluorescent stereo microscope 700. Fluorescent image data of the transgenic zebrafish in the placement tanks 302 is acquired by the fully-automatic fluorescent stereo microscope 700 and the image acquisition module 800, and the fluorescent image data is sent to the analysis module.

[0051] S3: The analysis module is used to receive the fluorescence image data and compare it with the fluorescence image data of normal aquatic organisms in the database, namely the fluorescence intensity of the central nervous system of zebrafish larvae and the length of the motor nerve synapse, so as to obtain the effects of different concentrations of CYN solution on transgenic zebrafish.

[0052] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. A system for analyzing the neurotoxicity of cyanobacteria toxins to aquatic organisms, comprising a bottom plate (100), characterized in that: Also includes: An electric guide rail mechanism (200) is provided on the bottom plate (100) and extends along the length direction of the bottom plate (100); a carrying container mechanism (300) is provided on a slider of the electric guide rail mechanism (200); the carrying container mechanism (300) includes a plate body (301); and a plurality of placement slots (302) for placing aquatic organisms are provided on the plate body (301); A first liquid supply mechanism (500) and a second liquid supply mechanism (600) are both provided on the bottom plate (100), wherein the first liquid supply mechanism (500) is used to supply CYN solutions of different concentrations into a plurality of groups of placement tanks (302), and the second liquid supply mechanism (600) is used to supply a fixation solution for fixing aquatic organisms into the placement tanks (302); and A fully automatic fluorescent stereo microscope (700) is provided on a bottom plate (100) and downstream of a second liquid supply mechanism (600); an image acquisition module (800) for acquiring fluorescent image data of aquatic organisms is provided on an eyepiece of the fully automatic fluorescent stereo microscope (700); the image acquisition module (800) is communicatively connected to an analysis module; the analysis module is configured to receive the fluorescent image data and compare it with fluorescent image data of normal aquatic organisms in a database, thereby obtaining an analysis result; The placement slots (302) are arranged in three rows and three columns. The bottom of the base plate (100) is provided with a box body (304) with a hollow top. The bottom of the box body (304) is provided with a connecting block (305) that is detachably connected to the slider of the electric guide rail mechanism (200). The slider and one end of the bottom of the box body (304) are connected by a telescopic device (306). The telescopic device (306) is used to drive the box body (304) to move relative to the connecting block (305) along its width direction. The bottom of the groove is provided with a plurality of groups of micro holes (303) communicating with the box body (304); a movable baffle (310) is provided at the bottom of the bottom plate (100) and in a position inside the box body (304); a through opening is provided on the movable baffle (310) at a position offset from the placement groove (302); the movable baffle (310) is connected to the box body (304) via a spring; an electromagnetic block (311) is embedded in the movable baffle (310); and a magnetic block is embedded in the bottom of the plate body (301) and is adsorbed and connected to the energized electromagnetic block (311).

2. The analysis system according to claim 1, wherein: The analysis system also includes: An incubator (400) is provided on the bottom plate (100) and between the first liquid supply mechanism (500) and the second liquid supply mechanism (600), both sides of the incubator (400) being hollow, and the incubator (400) is sleeved on the outside of the electric guide rail mechanism (200); and a closing mechanism (900), the closing mechanism (900) comprising: Two groups of door bodies (901) are slidably arranged on both sides of the incubator (400) and are used to close both sides of the incubator (400). The tops of the two groups of door bodies (901) are commonly connected to a bracket, and the bracket is connected to the top of the incubator (400) through a second telescopic device (902).

3. The analysis system according to claim 1, wherein: The first liquid supply mechanism (500) and the second liquid supply mechanism (600) have the same structure. The first liquid supply mechanism (500) comprises: A carrier (501) is provided on the bottom plate (100). The carrier (501) is provided with a plurality of groups of placement cylinders (502) for storing liquids. The number of the placement cylinders (502) corresponds to the number of a row of placement slots (302). A liquid outlet pipe (503) is provided at the bottom of each placement cylinder (502), and a solenoid valve (504) is provided on the liquid outlet pipe (503).

4. The analysis system according to claim 3, wherein: The side wall of the box body (304) is provided with a side plate (308) extending in the horizontal direction thereof, and the side plate (308) is integrally provided with a plurality of groups of arc-shaped protrusions (309), each of which is corresponding to a row of placement slots (302). The side wall of the carrier (501) is provided with a contact rod (505) for slidingly contacting with the top of the side plate (308) and the arc-shaped protrusion (309), and the outer wall of the contact rod (505) is provided with a contact rod for driving the contact rod (505). An elastic member (506) is reset downward, a conductive sheet 2 electrically connected to an external power supply is provided on the top of the contact rod (505), a conductive sheet 1 (507) electrically connected to the electromagnetic valve (504) is provided on the side wall of the support frame (501), and when the conductive sheet 1 (507) and the conductive sheet 2 are in contact, the bottom of the contact rod (505) contacts the highest point of the top of an arc-shaped protrusion (309), and the liquid outlet pipe (503) of the placement cylinder (502) corresponds to a row of placement slots (302).

5. The analysis system according to claim 4, characterized in that: A detection sensor (101) is provided on the bottom plate (100) and located between the incubator (400) and the second liquid supply mechanism (600). The detection sensor (101) is electrically connected to the controller and is used to send a signal to the controller when detecting that the carrying container mechanism (300) has reached a preset position, so that the controller controls the electromagnetic block (311) to be powered off.

6. The analysis system according to claim 1, wherein: The bottom plate (100) and the box body (304) are detachably connected.

7. The analysis system according to claim 1, wherein: The top of the slider of the electric guide rail mechanism (200) is provided with a slot adapted to the connecting block (305); a card rod is movably inserted into the side wall of the slider of the electric guide rail mechanism (200); and a card slot for the card block to enter is provided on the outer wall of the connecting block (305); an operating block (307) is provided at the outer end of the card rod, and a spring for driving the card rod to reset is sleeved on the outer wall of the card rod.

8. A method for analyzing the neurotoxicity of cyanobacteria toxins to aquatic organisms, utilizing the analysis system according to any one of claims 1 to 7, characterized in that: The following steps are involved: S1: Select several transgenic zebrafish with specific neural markers and place them in different placement tanks (302); S2: driving the carrying container mechanism (300) to the first liquid-feeding mechanism (500) by the electric guide rail mechanism (200), injecting CYN solutions of different concentrations into different placement slots (302) in sequence by the first liquid-feeding mechanism (500), after the transgenic zebrafish is exposed to the CYN solution for a preset time, driving the carrying container mechanism (300) to the second liquid-feeding mechanism (600) by the electric guide rail mechanism (200), injecting fixation solution into different placement slots (302) by the second liquid-feeding mechanism (600), fixing the transgenic zebrafish in the placement slots (302), and then driving the carrying container mechanism (300) to the fully automatic fluorescent stereo microscope (700) by the electric guide rail mechanism (200), acquiring fluorescent image data of the transgenic zebrafish in the placement slots (302) by the fully automatic fluorescent stereo microscope (700) and the image acquisition module (800), and sending the fluorescent image data to the analysis module; S3: The analysis module is used to receive the fluorescence image data and compare it with the fluorescence image data of normal aquatic organisms in the database, so as to obtain the effects of different concentrations of CYN solutions on transgenic zebrafish.

Images