Detection system suitable for AlphaLISA detection sample and working method thereof

Abstract

The invention discloses a detection system suitable for an AlphaLISA detection sample and a working method of the detection system, and relates to the technical field of AlphaLISA detection. The system comprises a laser device, a reflecting mirror, an AlphaLISA detection sample, a photomultiplier tube and a reflecting mirror position switching mechanism, and the reflecting mirror position switching mechanism is connected with the reflecting mirror. The position of the reflecting mirror is switched between a first position (A) and a second position (B); the working position (A) is used for enabling the light beam emitting end of the laser device, the reflecting surface of the reflecting mirror and the illuminated surface of the AlphaLISA detection sample to be sequentially communicated through light paths when the laser device emits a light beam, and the working position (B) is used for enabling the light beam emitting end of the laser device, the reflecting surface of the reflecting mirror and the illuminated surface of the AlphaLISA detection sample to be communicated through light paths; the non-working position is used for only enabling the light beam emergent surface of the AlphaLISA detection sample to be communicated with the light path of the light sensing surface of the photomultiplier tube after the laser stops emitting the light beam, so that the energy can be saved, and the hardware cost of the whole detection system can be reduced.

Description

Technical Field

[0001] The present invention belongs to the technical field of AlphaLISA detection, and in particular relates to a detection system suitable for AlphaLISA detection of samples and a working method thereof. Background Art

[0002] AlphaLISA detection technology is a homogeneous immunoassay method based on enhanced chemiluminescence; in detail, under the irradiation of laser (e.g., light with a wavelength of 680nm), the photosensitizer on the donor microbeads converts the oxygen in the surrounding environment (which is ground state oxygen) into more active singlet oxygen molecules, with a conversion rate of up to 60,000 per second. Before returning to ground state oxygen, the singlet oxygen molecule can diffuse 200nm in the solution, and if it encounters the acceptor microbead during the diffusion process, it will also react chemically with the chemiluminescent agent on it, thereby stimulating a series of chemiluminescent reactions and generating light signals with a wavelength range of about 615nm. Finally, the detection results can be obtained based on the light signals.

[0003] Existing detection schemes for AlphaLISA detection samples include Figure 1 As shown, it includes a laser 1, a first shutter 11, a spectroscope 20, an AlphaLISA detection sample 3, a second shutter 12 and a photomultiplier tube 4, wherein the laser 1 is used to emit a light beam (generally a red light beam) required for the AlphaLISA detection sample 3 to perform chemiluminescence, and the spectroscope 20 is used on the one hand to reflect the light beam from the laser 1 to the AlphaLISA detection sample 3, so that the AlphaLISA detection sample 3 emits another light beam (generally a green light beam) due to chemiluminescence after being irradiated by the light beam, and on the other hand to transmit the other light beam from the AlphaLISA detection sample 3 to the photomultiplier tube 4, so that the photomultiplier tube 4 obtains the raw data for quantifying the interaction between the target protein and the antibody fragment by detecting the other light beam. In addition, the first shutter 11 and the second shutter 12 are respectively used to cut off or conduct the corresponding light path to assist in completing the AlphaLISA detection.

[0004] The above existing detection scheme is based on the conventional modification of the fluorescence detection scheme, but the reflection and transmission efficiency of the spectroscope 20 cannot be close to 100% (generally only about 80%) due to material limitations, so it will have high requirements on the intensity of the laser beam and the detection sensitivity of the photomultiplier tube, resulting in energy waste and high hardware costs. In addition, considering that it is different from the principle of fluorescence luminescence (fluorescence luminescence refers to the fact that when dye molecules are excited by high-energy photons, the molecules emit photons with longer wavelength characteristics after nanoseconds due to energy level transitions), the time delay from laser excitation to signal generation in the AlphaLISA detection technology is relatively large, at the millisecond level (generally 20 milliseconds in actual applications), so laser reflection and chemiluminescence transmission do not need to be performed simultaneously like the fluorescence detection scheme.

[0005] In summary, how to provide a new detection scheme suitable for AlphaLISA detection of samples so as to reduce the requirements for laser intensity and detection sensitivity of photomultiplier tubes and achieve the purpose of saving energy and reducing hardware costs is a topic that technical personnel in this field urgently need to study. Summary of the invention

[0006] The purpose of the present invention is to provide a detection system suitable for AlphaLISA detection of samples and a working method thereof, so as to solve the problems of energy waste and high hardware cost in the existing detection schemes for AlphaLISA detection of samples.

[0007] In order to achieve the above object, the present invention adopts the following technical solutions:

[0008] In a first aspect, a detection system suitable for AlphaLISA detection samples is provided, comprising a laser, a reflector, an AlphaLISA detection sample, a photomultiplier tube, and a reflector position switching mechanism, wherein the laser is used to emit a first light beam required for the AlphaLISA detection sample to perform chemiluminescence, the reflector is used to reflect the first light beam to the AlphaLISA detection sample, and the photomultiplier tube is used to detect a second light beam emitted by the AlphaLISA detection sample due to chemiluminescence after being irradiated by the first light beam;

[0009] The reflector position switching mechanism is connected to the reflector and is used to switch the position of the reflector between the following first position (A) and second position (B):

[0010] (A) a working position for connecting only the beam emitting end of the laser, the reflecting surface of the reflecting mirror and the irradiated surface of the AlphaLISA detection sample in optical path sequence when the laser emits the first light beam;

[0011] (B) A non-working position for connecting only the beam exit surface of the AlphaLISA detection sample with the light path of the photosensitive surface of the photomultiplier tube after the laser stops emitting the first light beam, wherein the irradiated surface and the beam exit surface are the same surface.

[0012] Based on the above invention, a new detection scheme suitable for AlphaLISA detection samples is provided, which includes a laser, a reflector, an AlphaLISA detection sample, a photomultiplier tube and a reflector position switching mechanism, wherein the reflector position switching mechanism is connected to the reflector and is used to switch the position of the reflector between the following first position (A) and second position (B): (A) a working position for optically connecting only the beam emitting end of the laser, the reflecting surface of the reflector and the irradiated surface of the AlphaLISA detection sample in sequence when the laser emits a light beam; (B) a non-working position for optically connecting only the beam emitting surface of the AlphaLISA detection sample and the photosensitive surface of the photomultiplier tube after the laser stops emitting a light beam. In this way, while not affecting the transmission of the two light beams and ensuring the smooth completion of the AlphaLISA detection, the demand for laser intensity can be reduced, saving about 20% of energy, and reducing the demand for detection sensitivity of the photomultiplier tube. There is no need to configure a shutter and an expensive spectroscope, thereby reducing the hardware cost of the entire detection system, which is convenient for practical application and promotion.

[0013] In a possible design, the reflector position switching mechanism includes a motor, wherein the output shaft of the motor is radially connected to the reflector, and the axis of the output shaft is perpendicular to the reflective surface of the reflector.

[0014] In a possible design, the reflector position switching mechanism also includes a linkage controller, wherein the input end of the linkage controller is communicatively connected to the working status output end of the laser, and the controlled end of the motor is communicatively connected to the output end of the linkage controller.

[0015] In a possible design, a first plano-convex lens is provided between the reflective surface of the reflector and the illuminated surface of the AlphaLISA detection sample, wherein the plane of the first plano-convex lens faces the illuminated surface.

[0016] In a possible design, a second plano-convex lens is provided between the light beam exit surface of the AlphaLISA detection sample and the photosensitive surface of the photomultiplier tube, wherein the plane of the second plano-convex lens faces the light beam exit surface.

[0017] In one possible design, a filter is provided between the light beam exit surface of the AlphaLISA detection sample and the photosensitive surface of the photomultiplier tube, wherein the filter is not located between the reflective surface of the reflector and the irradiated surface of the AlphaLISA detection sample when the reflector is in the first position (A).

[0018] In a possible design, when there are two photomultiplier tubes, namely the first photomultiplier tube and the second photomultiplier tube, a beam splitter is provided between the light beam exit surface of the AlphaLISA detection sample and the photosensitive surface of the photomultiplier tube, wherein the beam splitter is not located between the reflective surface of the reflector and the irradiated surface of the AlphaLISA detection sample when the reflector is in the first position (A);

[0019] The reflective surface of the beam splitter is optically connected to the photosensitive surface of the first photomultiplier tube, and the transmissive surface of the beam splitter is optically connected to the photosensitive surface of the second photomultiplier tube.

[0020] In one possible design, a first filter is provided between the reflective surface of the spectroscope and the photosensitive surface of the first photomultiplier tube, and a second filter is provided between the transmissive surface of the spectroscope and the photosensitive surface of the second photomultiplier tube, wherein the first filter and the second filter have different filtering characteristics.

[0021] In a possible design, when the direction of the second light beam is vertically upward, the photosensitive surface of the first photomultiplier tube is vertically arranged, and the photosensitive surface of the second photomultiplier tube is horizontally arranged and downward, the beam splitter is arranged at a 45-degree tilt.

[0022] In a possible design, when the direction of the first light beam is horizontal and the irradiated surface of the AlphaLISA detection sample is horizontal and facing upward, the reflector is tilted at 45 degrees when in the first position (A).

[0023] In a second aspect, a method for operating a detection system as described in the first aspect or any possible design of the first aspect and suitable for AlphaLISA detection of samples is provided, comprising:

[0024] After placing the AlphaLISA detection sample, starting the laser to emit the first light beam, and simultaneously placing the reflector in the first position (A) through the reflector position switching mechanism;

[0025] When the emission duration of the first light beam reaches a first preset duration, stopping the laser;

[0026] When the laser stop time reaches a second preset time, the reflector position switching mechanism switches the reflector to the second position (B), and simultaneously starts the photomultiplier tube to detect the second light beam;

[0027] When the start-up time of the photomultiplier tube reaches a third preset time, the photomultiplier tube is stopped.

[0028] In one possible design, the first preset time length is between 300 and 700 milliseconds, the second preset time length is between 15 and 20 milliseconds, and the third preset time length is between 200 and 400 milliseconds.

[0029] Beneficial effects of the above scheme:

[0030] (1) The present invention provides a novel detection scheme suitable for AlphaLISA detection samples, including a laser, a reflector, an AlphaLISA detection sample, a photomultiplier tube and a reflector position switching mechanism, wherein the reflector position switching mechanism is connected to the reflector and is used to switch the position of the reflector between the following first position (A) and second position (B): (A) a working position for connecting only the beam emitting end of the laser, the reflecting surface of the reflector and the irradiated surface of the AlphaLISA detection sample in optical path sequence when the laser emits a light beam; (B) a non-working position for connecting only the beam emitting surface of the AlphaLISA detection sample and the photosensitive surface of the photomultiplier tube in optical path sequence after the laser stops emitting a light beam, thereby not affecting the transmission of the two light beams and ensuring the smooth completion of the AlphaLISA detection, while also reducing the demand for laser intensity, saving about 20% of energy, and reducing the demand for detection sensitivity of the photomultiplier tube, and also eliminating the need to configure a shutter and an expensive spectroscope, thereby reducing the hardware cost of the entire detection system, facilitating practical application and promotion;

[0031] (2) It can also be applied to dual-channel or even multi-channel detection applications, which further facilitates practical application and promotion. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0033] Figure 1 It is a schematic diagram of the structure of the existing detection system for AlphaLISA detection samples.

[0034] Figure 2 A schematic diagram of the structure of a single-channel detection system for AlphaLISA detection of samples provided in an embodiment of the present application before and after the mirror position is switched, wherein: Figure 2 (a) shows a schematic diagram of the structure of the single-channel detection system when the reflector position is switched to the working position. Figure 2 (b) shows a schematic diagram of the structure of the single-channel detection system when the reflector position is switched to a non-working position.

[0035] Figure 3 A schematic diagram of the structure of a dual-channel detection system for AlphaLISA detection of samples provided in an embodiment of the present application before and after the mirror position is switched, wherein: Figure 3 (a) shows the schematic diagram of the structure of the dual-channel detection system when the reflector position is switched to the working position. Figure 3 (b) shows a schematic diagram of the structure of the dual-channel detection system when the reflector position is switched to a non-working position.

[0036] In the above drawings: 1-laser; 11-first shutter; 12-second shutter; 2-reflector; 20-beam splitter; 3-AlphaLISA detection sample; 4-photomultiplier tube; 51-motor; 510-output shaft; 52-linkage controller; 61-first plano-convex lens; 62-second plano-convex lens; 7-filter; 71-first filter; 72-second filter. DETAILED DESCRIPTION

[0037] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the present invention will be briefly introduced below in combination with the drawings and the description of the embodiments or the prior art. Obviously, the following description of the structures of the drawings is only some embodiments of the present invention. For ordinary technicians in this field, other embodiments can be obtained based on these embodiments without creative work. It should be noted that the description of these embodiments is used to help understand the present invention, but does not constitute a limitation of the present invention.

[0038] It should be understood that although the terms first and second, etc. may be used herein to describe various objects, these objects should not be limited by these terms. These terms are only used to distinguish one object from another object. For example, a first object can be referred to as a second object, and similarly, a second object can be referred to as a first object without departing from the scope of the exemplary embodiments of the present invention.

[0039] It should be understood that the term "and / or" that may appear in this document is merely a description of the association relationship between associated objects, indicating that there may be three relationships. For example, A and / or B can indicate three situations: A exists alone, B exists alone, or A and B exist at the same time. For another example, A, B and / or C can indicate the existence of any one of A, B and C or any combination of them. The term " / and" that may appear in this document describes another type of association object relationship, indicating that there may be two relationships. For example, A / and B can indicate two situations: A exists alone or A and B exist at the same time. In addition, the character " / " that may appear in this document generally indicates that the previous and next associated objects are in an "or" relationship.

[0040] Embodiment 1

[0041] like Figure 2 As shown, the detection system provided in this embodiment and applicable to AlphaLISA detection samples includes but is not limited to a laser 1, a reflector 2, an AlphaLISA detection sample 3, a photomultiplier tube 4 and a reflector position switching mechanism, wherein the laser 1 is used to emit a first light beam required for the AlphaLISA detection sample 3 to perform chemiluminescence, the reflector 2 is used to reflect the first light beam to the AlphaLISA detection sample 3, the photomultiplier tube 4 is used to detect a second light beam emitted by the AlphaLISA detection sample 3 due to chemiluminescence after being irradiated by the first light beam; the reflector The position switching mechanism is connected to the reflector 2 and is used to switch the position of the reflector 2 between the following first position (A) and second position (B): (A) a working position for optically connecting only the beam emitting end of the laser 1, the reflecting surface of the reflector 2 and the irradiated surface of the AlphaLISA detection sample 3 in sequence when the laser 1 emits the first light beam; (B) a non-working position for optically connecting only the beam emitting surface of the AlphaLISA detection sample 3 and the photosensitive surface of the photomultiplier tube 4 after the laser 1 stops emitting the first light beam, wherein the irradiated surface and the beam emitting surface are the same surface.

[0042] like Figure 2 As shown, in the specific structure of the detection system, the laser 1, the AlphaLISA detection sample 3 and the photomultiplier tube 4 are all conventional configurations of existing detection schemes, wherein the first light beam emitted by the laser 1 can be, but not limited to, a red light beam, and the second light beam emitted by the AlphaLISA detection sample 3 due to chemiluminescence can be, but not limited to, a green light beam. The reflective surface of the reflector 2 can be, but not limited to, a silver surface to achieve close to 100% light reflection; specifically, as Figure 2As shown in (a), when the direction of the first light beam is horizontal and the irradiated surface of the AlphaLISA detection sample 3 is horizontal and facing upward, the reflector 2 is set at a 45-degree tilt when in the first position (A). Since the time delay from laser excitation to signal generation in the AlphaLISA detection technology is relatively large, the first light beam and the second light beam are emitted asynchronously (i.e., the first light beam is emitted first, and then the second light beam is emitted about 20 milliseconds after the first light beam is emitted). Therefore, the position of the reflector 2 is switched between the first position (A) and the second position (B) by the reflector position switching mechanism. This can not only avoid affecting the transmission of the first light beam and the second light beam and ensure the smooth completion of the AlphaLISA detection, but also reduce the demand for laser intensity and save about 20% of energy because the reflector 2 has a higher reflection efficiency than the spectroscope 20 (generally about 20% higher). Moreover, because the reflector 2 has been removed from the working position when the second light beam is emitted, the second light beam can be transmitted to the photomultiplier tube 4 without hindrance (i.e., it has a transmission efficiency of 100%, which is equivalent to an increase of 20%), reducing the demand for the detection sensitivity of the photomultiplier tube (because the intensity of the second light beam is increased by about 20%). In addition, there is no need to configure a shutter and an expensive spectroscope, thereby reducing the hardware cost of the entire detection system and facilitating practical application and promotion.

[0043] Specifically, the working method of the detection system suitable for AlphaLISA detection of samples includes but is not limited to the following steps S1 to S4.

[0044] S1. After placing the AlphaLISA detection sample 3, start the laser 1 to emit the first light beam, and at the same time, use the reflector position switching mechanism to place the reflector 2 in the first position (A).

[0045] In the step S1, the specific placement method of the AlphaLISA detection sample 3 can be a manual placement method or an automatic placement method using an automated mechanical device. Since the reflector 2 has been placed in the first position (A) by the reflector position switching mechanism, it can be ensured that the first light beam is irradiated onto the AlphaLISA detection sample 3 after passing through the reflective surface of the reflector 2, so as to promote the chemiluminescence of the AlphaLISA detection sample 3. In addition, the reflector 2 can be placed in the first position (A) by the reflector position switching mechanism, and then the laser 1 is started to emit the first light beam.

[0046] S2. When the emission duration of the first light beam reaches a first preset duration, stop the laser 1.

[0047] In step S2, the first preset duration is the laser excitation duration of the laser 1, which can be changed according to the detection system and reagent application. Specifically, the first preset duration is between 300 and 700 milliseconds. For example, the first preset duration is designed to be 500 milliseconds.

[0048] S3. When the stop time of the laser 1 reaches a second preset time, the reflector 2 is switched to the second position (B) through the reflector position switching mechanism, and the photomultiplier tube 4 is started to detect the second light beam at the same time.

[0049] In step S3, since the reflector 2 has been switched to the second position (B) by the reflector position switching mechanism, it can be ensured that the second light beam is transmitted to the photomultiplier tube 4 without hindrance, so as to successfully complete the AlphaLISA detection. The second preset time length can be set according to the time length required for the AlphaLISA to detect the chemiluminescence of the sample 3. Specifically, the second preset time length is between 15 and 20 milliseconds, for example, the second preset time length is designed to be 18 milliseconds.

[0050] S4. When the activation time of the photomultiplier tube 4 reaches a third preset time, the photomultiplier tube 4 is stopped.

[0051] In step S4, the third preset time is the detection reading time of the photomultiplier tube 4, which may also be changed according to the detection system and reagent application. Specifically, the third preset time is between 200 and 400 milliseconds. For example, the third preset time is designed to be 300 milliseconds.

[0052] Specifically, the reflector position switching mechanism includes but is not limited to a motor 51, wherein the output shaft 510 of the motor 51 is radially connected to the reflector 2, and the axis of the output shaft 510 is perpendicular to the reflective surface of the reflector 2. Figure 2 As shown in (a) and (b), the motor 51 is also arranged in a 45-degree tilted manner, and by driving the motor 51 to rotate the output shaft 510, the reflector 2 can be driven to rotate, thereby realizing the purpose of the reflector position switching mechanism.

[0053] More specifically, the reflector position switching mechanism further includes but is not limited to a linkage controller 52, wherein the input end of the linkage controller 52 is communicatively connected to the working state output end of the laser 1, and the controlled end of the motor 51 is communicatively connected to the output end of the linkage controller 52. The linkage controller 52 is used to control the reflector position switching mechanism according to the working state of the laser 1, so that the reflector 2 is in the first position (A) when the laser 1 emits the first light beam, and the reflector 2 is in the second position (B) after the laser 1 stops emitting the first light beam. The linkage controller 52 is specifically but not limited to a microcontroller chip or an FPGA (Field Programmable Gate Array) chip with a timing function.

[0054] Preferably, a first plano-convex lens 61 is provided between the reflective surface of the reflector 2 and the illuminated surface of the AlphaLISA detection sample 3, wherein the plane of the first plano-convex lens 61 faces the illuminated surface. Figure 2 As shown in (a), the first plano-convex lens 61 is used to focus the first light beam so as to avoid the influence of the chemiluminescence efficiency of the AlphaLISA detection of the sample 3 due to the divergence of light.

[0055] Preferably, a second plano-convex lens 62 is provided between the light beam exit surface of the AlphaLISA detection sample 3 and the photosensitive surface of the photomultiplier tube 4, wherein the plane of the second plano-convex lens 62 faces the light beam exit surface. Figure 2 As shown in (b) of FIG. 5 , the second plano-convex lens 62 is used to gather the second light beam so as to avoid the influence of the detection effect of the photomultiplier tube 4 on the detection effect of the photomultiplier tube 4 due to the divergence of the light beam. Figure 2 As shown, the second plano-convex lens 62 and the first plano-convex lens 61 are further preferably the same plano-convex lens to reduce hardware cost and structural complexity.

[0056] Preferably, a filter 7 is provided between the light beam exit surface of the AlphaLISA detection sample 3 and the photosensitive surface of the photomultiplier tube 4, wherein the filter 7 is not located between the reflective surface of the reflector 2 and the irradiated surface of the AlphaLISA detection sample 3 when the reflector 2 is in the first position (A). Figure 2 As shown, the filter 7 is used to filter out other light except the second light beam, so as to ensure the detection effect of the photomultiplier tube 4.

[0057] In summary, the detection system provided by this embodiment has the following technical effects:

[0058] (1) This embodiment provides a novel detection scheme suitable for AlphaLISA detection samples, including a laser, a reflector, an AlphaLISA detection sample, a photomultiplier tube and a reflector position switching mechanism, wherein the reflector position switching mechanism is connected to the reflector and is used to switch the position of the reflector between the following first position (A) and second position (B): (A) a working position for connecting only the beam emitting end of the laser, the reflecting surface of the reflector and the irradiated surface of the AlphaLISA detection sample in optical path sequence when the laser emits a light beam; (B) a non-working position for connecting only the beam emitting surface of the AlphaLISA detection sample and the photosensitive surface of the photomultiplier tube in optical path sequence after the laser stops emitting a light beam. In this way, while not affecting the transmission of the two light beams and ensuring the smooth completion of the AlphaLISA detection, the requirement for laser intensity can be reduced, saving about 20% of energy, and reducing the requirement for detection sensitivity of the photomultiplier tube. There is no need to configure a shutter and an expensive spectroscope, thereby reducing the hardware cost of the entire detection system, facilitating practical application and promotion.

[0059] Embodiment 2

[0060] Based on the technical solution of the first embodiment, this embodiment further provides a dual-channel detection system expanded from the single-channel detection system. Figure 3 As shown, the difference between this embodiment and the single-channel detection system described in the first embodiment is that: the number of the photomultiplier tubes 4 is two, namely the first photomultiplier tube 41 and the second photomultiplier tube 42; a spectroscope 20 is provided between the light beam exit surface of the AlphaLISA detection sample 3 and the photosensitive surface of the photomultiplier tube 4, wherein the spectroscope 20 is not located between the reflective surface of the reflector 2 and the irradiated surface of the AlphaLISA detection sample 3 when the reflector 2 is in the first position (A); the reflective surface of the spectroscope 20 is connected to the photosensitive surface optical path of the first photomultiplier tube 41, and the transmission surface of the spectroscope 20 is connected to the photosensitive surface optical path of the second photomultiplier tube 42. Figure 3 As shown, the beam splitter 20 is used to subdivide the second light beam into two different light beams, so that the two light beams are respectively incident on the first photomultiplier tube 41 and the second photomultiplier tube 42 in a one-to-one correspondence, so as to achieve different AlphaLISA detection purposes; in detail, as shown Figure 3As shown, when the direction of the second light beam is vertically upward, the photosensitive surface of the first photomultiplier tube 41 is vertically arranged, and the photosensitive surface of the second photomultiplier tube 42 is horizontally arranged and downwardly arranged, the beam splitter 20 is arranged in a 45-degree tilted manner. In addition, in order to filter other non-target light to ensure different detection effects, preferably, a first filter 71 is arranged between the reflective surface of the beam splitter 20 and the photosensitive surface of the first photomultiplier tube 41, and a second filter 72 is arranged between the transmission surface of the beam splitter 20 and the photosensitive surface of the second photomultiplier tube 42, wherein the first filter 71 and the second filter 72 have different filtering characteristics.

[0061] In summary, based on the technical effect of the first embodiment, this embodiment also has the following technical effects: (1) It can also be applied to dual-channel or even multi-channel detection applications, further facilitating practical application and promotion.

[0062] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the protection scope of the present invention. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A detection system suitable for AlphaLISA detection of samples, characterized in that: The invention comprises a laser (1), a reflector (2), an AlphaLISA detection sample (3), a photomultiplier tube (4) and a reflector position switching mechanism, wherein the laser (1) is used to emit a first light beam required for the AlphaLISA detection sample (3) to perform chemiluminescence, the reflector (2) is used to reflect the first light beam onto the AlphaLISA detection sample (3), and the photomultiplier tube (4) is used to detect a second light beam emitted by the AlphaLISA detection sample (3) due to chemiluminescence after being irradiated by the first light beam; The reflector position switching mechanism is connected to the reflector (2) and is used to switch the position of the reflector (2) between the following first position (A) and second position (B): (A) a working position for connecting only the beam emitting end of the laser (1), the reflecting surface of the reflecting mirror (2) and the irradiated surface of the AlphaLISA detection sample (3) in optical path sequence when the laser (1) emits the first light beam; (B) a non-working position for connecting only the beam exit surface of the AlphaLISA detection sample (3) with the light path of the photosensitive surface of the photomultiplier tube (4) after the laser (1) stops emitting the first light beam, wherein the irradiated surface and the beam exit surface are the same surface.

2. The detection system according to claim 1, characterized in that: The reflector position switching mechanism comprises a motor (51), wherein an output shaft (510) of the motor (51) is radially connected to the reflector (2), and an axis of the output shaft (510) is perpendicular to a reflective surface of the reflector (2).

3. The detection system according to claim 2, characterized in that: The reflector position switching mechanism also includes a linkage controller (52), wherein an input end of the linkage controller (52) is communicatively connected to an output end of a working state of the laser (1), and a controlled end of the motor (51) is communicatively connected to an output end of the linkage controller (52).

4. The detection system according to claim 1, characterized in that: A first plano-convex lens (61) is provided between the reflective surface of the reflector (2) and the illuminated surface of the AlphaLISA detection sample (3), wherein the plane of the first plano-convex lens (61) faces the illuminated surface.

5. The detection system according to claim 1, characterized in that: A second plano-convex lens (62) is provided between the light beam exit surface of the AlphaLISA detection sample (3) and the photosensitive surface of the photomultiplier tube (4), wherein the plane of the second plano-convex lens (62) faces the light beam exit surface.

6. The detection system according to claim 1, characterized in that: A filter (7) is provided between the light beam exit surface of the AlphaLISA detection sample (3) and the photosensitive surface of the photomultiplier tube (4), wherein the filter (7) is not located between the reflection surface of the reflection mirror (2) and the irradiated surface of the AlphaLISA detection sample (3) when the reflection mirror (2) is in the first position (A).

7. The detection system according to claim 1, characterized in that: When the number of the photomultiplier tubes (4) is two and they are respectively a first photomultiplier tube (41) and a second photomultiplier tube (42), a beam splitter (20) is provided between the light beam exit surface of the AlphaLISA detection sample (3) and the photosensitive surface of the photomultiplier tube (4), wherein the beam splitter (20) is not located between the reflection surface of the reflection mirror (2) and the irradiated surface of the AlphaLISA detection sample (3) when the reflection mirror (2) is in the first position (A); The reflective surface of the beam splitter (20) is optically connected to the photosensitive surface of the first photomultiplier tube (41), and the transmissive surface of the beam splitter (20) is optically connected to the photosensitive surface of the second photomultiplier tube (42).

8. The detection system according to claim 7, characterized in that: A first filter (71) is provided between the reflective surface of the spectroscope (20) and the photosensitive surface of the first photomultiplier tube (41), and a second filter (72) is provided between the transmissive surface of the spectroscope (20) and the photosensitive surface of the second photomultiplier tube (42), wherein the first filter (71) and the second filter (72) have different filtering characteristics.

9. A method for using the AlphaLISA detection system for detecting samples according to any one of claims 1 to 8, comprising: After placing the AlphaLISA detection sample (3), starting the laser (1) to emit the first light beam, and simultaneously placing the reflector (2) in the first position (A) through the reflector position switching mechanism; When the emission duration of the first light beam reaches a first preset duration, stopping the laser (1); When the stop time of the laser (1) reaches a second preset time, the reflector position switching mechanism switches the reflector (2) to the second position (B), and simultaneously starts the photomultiplier tube (4) to detect the second light beam; When the activation time of the photomultiplier tube (4) reaches a third preset time, the photomultiplier tube (4) is stopped.

10. The working method according to claim 9, characterized in that: The first preset time length is between 300 and 700 milliseconds, the second preset time length is between 15 and 20 milliseconds, and the third preset time length is between 200 and 400 milliseconds.

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