Exiguobacterium acetylbacter and application thereof
Through the combined application of acetyl microbacterium isolated from the algae and algae, the problem of COD and total phosphorus removal in the tail water of the sewage treatment plant was solved, the deep purification of sewage was achieved, and the sustainable development of the ecological environment and economy was promoted.
Patent Information
- Application Number
- CN202411752161.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-02
- Publication Date
- 2025-02-21
AI Technical Summary
Indices such as COD and total phosphorus in the tail water of sewage treatment plants are significantly higher than those of V-type water in surface water, resulting in water resource shortage and water environmental pollution. It is difficult for the existing technology to effectively remove these pollutants.
By isolating a kind of acetylicum (Exiguobacterium acetylicum) from the submerged plant Knoxa algae and applying it in combination with Knoxa algae to the tail water of the sewage treatment plant, microbial activity is improved to deeply remove total phosphorus and chemical oxygen demand.
It effectively improves the growth efficiency of aquatic plants, significantly removes the total phosphorus and chemical oxygen demand in the tail water of sewage treatment plants, and achieves the effect of deep purification of water quality, which is conducive to the sustainable development of the ecological environment and economy.
Smart Images
Figure CN119490937A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the field of biotechnology, in particular to an acetylated Exiguobacterium and its application. Background Art
[0002] With the rapid economic development of modern society and the increasing frequency of human activities, water pollution, especially nutrient overload, has become increasingly serious, leading to severe water shortages and attracting widespread attention from the world. To this end, my country has built a large number of sewage treatment plants to reduce water pollution. However, at present, indicators such as COD and total phosphorus discharged by sewage treatment plants are significantly higher than those of Class V surface water. How to deeply purify the tail water of sewage treatment plants, reduce the impact on receiving natural water bodies, and protect water resources is one of the main problems currently faced.
[0003] At present, the deep treatment technologies for tailwater from sewage treatment plants mainly include physical and chemical treatment technologies and biological treatment methods. Among them, physical and chemical technologies such as adsorption and precipitation are simple to operate and highly efficient, but they have disadvantages such as limited adsorption capacity, possible secondary pollution, and high cost. Biological treatment technology uses the ability of plants and microorganisms to absorb and decompose nutrients to treat pollutants in sewage to achieve the purpose of purifying water quality. Biological treatment technology has the advantages of being environmentally friendly and low cost, so it is a very promising tailwater treatment method.
[0004] As a kind of biological technology for deep treatment of tailwater from sewage treatment plants developed in recent years, artificial wetlands have the characteristics of good effluent quality, low investment, simple structure, convenient operation and management, and low operating costs. Among them, microorganisms are the main contributors to the treatment of pollutants in artificial wetlands. Enhancing microbial activity can effectively enhance the operating efficiency of artificial wetlands. Current studies have shown that the ability of artificial wetlands to remove pollutants can be enhanced by adding nutrients or inoculating exogenous microorganisms. Although the addition of nutrients improves the denitrification capacity of artificial wetlands, its ability to remove other pollutants is still weak; although there are abundant microbial species in artificial wetlands, the number of microorganisms with specific functions and adaptability to different environmental conditions is limited. Therefore, the separation, screening and domestication of degrading bacteria with efficient degradation ability remains one of the key tasks of future research. Summary of the invention
[0005] The purpose of the present invention is to provide an acetylated Exiguobacterium and its application in the tail water of a sewage treatment plant to solve the problems raised in the above background technology. To solve the above technical problems, the technical solution adopted by the present invention is: a strain of Exiguobacterium acetylicum, Latin name: Exiguobacterium acetylicum, microbial classification named Exiguobacterium acetylicum, preserved in the General Microbiological Center of China Microbiological Culture Collection Administration (CGMCC), with a preservation number of CGMCC NO.32798.
[0006] As a further solution of the present invention, the preparation of Exiguobacterium acetylicum is carried out in the following steps: S1. Sample collection: Samples were collected from the submerged plant Ceratophyllum glabra in natural wetlands; S2. Bacteria screening: Prepare LB selection medium and screen microorganisms by reducing nutrients; S3. Strain isolation: Cultivate the strain using the prepared medium, continuously pick single colonies for inoculation to obtain pure culture, and preserve it; S4. Strain identification: The strains were identified by 16S rDNA.
[0007] As a further solution of the present invention, the strain in S4 is preserved in a -80°C refrigerator using glycerol.
[0008] The invention discloses an application of Exiguobacterium acetylicum in the treatment of tail water of a sewage treatment plant and aquaculture tail water. The Exiguobacterium acetylicum is combined with a submerged plant Ceratophyllum dactylon to deeply remove nutrients in the tail water of the sewage treatment plant.
[0009] As a further embodiment of the present invention, the content of acetylated Exiguobacterium acetyl is not less than 10 3 cfu / mL.
[0010] As a further embodiment of the present invention, the nutrients are total phosphorus and chemical oxygen demand.
[0011] Since the present invention adopts the above technical scheme, the present invention has the advantages and positive effects of: acetylated Microbacterium is isolated from the commonly used plant duckweed in artificial wetlands, and the bacteria are combined with the submerged plant duckweed to be applied to the deep treatment of sewage treatment plants and aquaculture tail water, which can effectively improve the growth efficiency of aquatic plants, remove nutrients in the water body, achieve the effect of deep purification of water quality, and is beneficial to the sustainable development of the ecological environment and economy. BRIEF DESCRIPTION OF THE DRAWINGS
[0012] Figure 1 This is the colony morphology of Exiguobacterium acetylicum.
[0013] Figure 2 For the removal effect of nutrients in water. DETAILED DESCRIPTION Example
[0014] like Figure 1 As shown, the present invention provides an acetylated Exiguobacterium and its application in tail water of a sewage treatment plant, wherein an acetylated Exiguobacterium is separated from a biofilm attached to a submerged plant, Ceratophyllum davidianum, to form a microorganism capable of degrading nutrients; S1. Sample collection: Take a certain amount of Ceratophyllum glabrae plants, treat them twice with sterile PBS buffer under ultrasonic conditions (25℃, 40KHZ) for 5 min to elute the attached microorganisms, and centrifuge at 10000 r / min for 10 min to obtain precipitated bacteria; S2. Screening of strains: Prepare LB medium, further configure the selection medium with the reduction of total nitrogen, ammonia nitrogen, total phosphorus and chemical oxygen demand as indicators, screen microorganisms: Take the precipitated bacteria from S1 and dilute them with sterile water to make 10 -1 , 10 -2 , 10 -3 , 10 -4 and 10 -5 Dilution, after the culture medium solidifies, take 100 μL of each gradient dilution and evenly spread it on the selection culture medium plate, set up 3 replicates. The plate is incubated at 26°C for 24 hours in an inverted manner. After the colonies grow and form, screen out the colonies with significant differences in colony morphology, size, color and other characteristics, and pick them and transfer them to a plate with the same growth conditions; S3. Strain isolation: Through isolation culture-single colony isolation, pick a single colony obtained in the isolation medium, streak it on the isolation medium plate, culture it for 12-24 hours, and then pick a single colony and inoculate it into the isolation medium to obtain pure culture. The strain is preserved by glycerol method in a -80℃ refrigerator, and the strain identification is carried out at the same time; S4. Strain identification: The strain was sequenced by 16S rDNA sequencing.
[0015] In this embodiment, Exiguobacterium acetylicum is combined with submerged plants to remove nutrients from tail water of a sewage treatment plant.
[0016] In this embodiment, the activation culture step of Exiguobacterium acetylicum is as follows: taking out the frozen strain, thawing at room temperature, and streaking on a solid culture medium plate (culture temperature is 26°C, duration is 24 h). Pick a single colony, inoculate it into a conical flask of liquid culture medium, and shake culture (constant temperature of 37°C, speed of 300 rpm) until its optical density reaches 1. Then centrifuge (10000 r / min, 10 min) to obtain the precipitated strain for use.
[0017] In this embodiment, the content of acetyl bacteria added to the water body is not less than 10 3 cfu / mL.
[0018] In this embodiment, the water quality of the simulated sewage treatment plant tail water is shown in the following table (mg / L): Table 1 Water quality of simulated sewage treatment plant tailwater In this embodiment, the submerged plant is Ceratophyllum.
[0019] In this embodiment, the experiment of treating tail water of a sewage treatment plant with Exiguobacterium acetylicum and Duckweed was divided into a control group (without Exiguobacterium acetylicum and Duckweed) and an experimental group (with Exiguobacterium acetylicum and Duckweed).
[0020] In this embodiment, the combined treatment time is 4 days.
[0021] In this embodiment, Figure 2 As shown in Figure 2, the removal of various nutrient indicators when the tail water of the sewage treatment plant was treated with acetyl bacteria combined with hornwort for 4 days was shown in Figure 2. Figure 2 The removal rates of total nitrogen, ammonia nitrogen, total phosphorus and chemical oxygen demand in the control group were 66.28%, 87.91%, 70.31% and 53.67%, respectively, while those in the treatment group were 68.88%, 88.09%, 79.02% and 66.87%, respectively. The treatment with Exiguobacterium acetylicum combined with Dermatophyte can effectively remove substances in the effluent of the sewage treatment plant, and the removal capacity is improved compared with the control, especially for total phosphorus and chemical oxygen demand.
[0022] Although specific embodiments of the present invention are described above, those skilled in the art should understand that these are merely examples and that various changes or modifications may be made to the embodiments without departing from the principles and essence of the present invention. The scope of protection of the present invention is limited only by the appended claims.
Claims
1. A strain of Exiguobacterium acetylicum, characterized in that: The microorganism was classified and named Exiguobacterium acetylicum and deposited in the General Microbiology Center of China Microorganism Culture Collection Administration with the deposit number: CGMCC NO.32798.
2. The strain of Exiguobacterium acetylicum according to claim 1, characterized in that: The preparation of the acetylated Exiguobacterium follows the following steps: S1. Sample collection: Samples were collected from the submerged plant Ceratophyllum glabra in natural wetlands; S2. Bacteria screening: Prepare LB selection medium and screen microorganisms by reducing nutrients; S3. Strain isolation: Cultivate the strain using the prepared medium, continuously pick single colonies for inoculation to obtain pure culture, and preserve it; S4. Strain identification: The strains were identified by 16S rDNA.
3. The strain of Exiguobacterium acetylicum according to claim 2, characterized in that: The strain S4 was preserved in a -80°C refrigerator using glycerol.
4. An application of Exiguobacterium acetylicum as claimed in any one of claims 1 to 3 in the treatment of tail water from a sewage treatment plant and aquaculture tail water, characterized in that: The acetylated Exiguobacterium is combined with the submerged plant Ceratophyllum davidianum to deeply remove nutrients in the tail water of a sewage treatment plant.
5. The use of Exiguobacterium acetylicum according to claim 4, characterized in that: Add acetyl bacteria to the water in an amount not less than 10 3 cfu / mL.
6. The use of Exiguobacterium acetylicum according to claim 4, characterized in that: The nutrients are total phosphorus and chemical oxygen demand.