Kit for dissociating and detecting 25-hydroxyvitamin D and detection method thereof

Abstract

The invention provides a 25-hydroxyvitamin D dissociation and detection kit and a detection method thereof. By screening and optimizing formulas of a dissociation agent and antibody dry powder, automatic uniform mixing and dissociation of a sample are realized, the method is not influenced by human factors and does not depend on any uniform mixing equipment, and sufficient reaction of a 25-hydroxyvitamin D antibody and an object to be detected is ensured; meanwhile, the operation process of an immunochromatography method is improved, the dissociation process of a sample and the binding process of the 25-hydroxyvitamin D antibody and a to-be-detected substance are both completed in one reaction tube and are completely separated from a test strip, after the reaction is completed, the test strip is adopted for sample injection detection, meanwhile, a specific quality control line is arranged, and the detection accuracy is improved. The accuracy and the sensitivity of portable detection of the 25-hydroxy vitamin D by the immunochromatography are remarkably improved, and the detection sensitivity of the 25-hydroxy vitamin D reaches 1ng / mL.

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