Apparatus and method for stimulated raman detection

Abstract

The application discloses a device and a method for stimulated Raman detection, and the technical scheme is as follows: a femtosecond stimulated Raman spectrometer body is arranged, support frames are arranged on the two sides of the femtosecond stimulated Raman spectrometer body, a side plate is arranged on one side of the support frame, a shielding plate is arranged on one side of the side plate, observation holes are arranged on one side of the side plate and the shielding plate respectively, and the femtosecond stimulated Raman spectrometer body, the support frame, the side plate, the shielding plate, the observation hole, the transparent plate, the operation column, the temperature rising frame, the heating wire, the blocking net, the metal net, the support frame and the micro fan are used in cooperation, so that the water vapor on the surface of the optical element of the femtosecond stimulated Raman spectrometer body is prevented from condensing due to a high-humidity environment, the light path transmission and the signal strength are affected, meanwhile, the increase of the humidity is prevented from causing the increase of the water content in the sample, so that the molecular structure and the vibration mode of the sample are changed, and then the generation and transmission of the Raman signal are affected.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of stimulated Raman detection, and particularly relates to a device and method for stimulated Raman detection. BACKGROUND

[0002] Stimulated Raman scattering refers to the strong interaction between high-intensity laser and molecular matter, so that the scattering process has the nature of stimulated emission. This scattered light is Raman scattered light, so this nonlinear optical effect is called stimulated Raman scattering. Stimulated Raman scattering exhibits threshold characteristics, just like a laser, which can only be generated with appropriate pump power. Stimulated Raman scattering microscopic imaging technology uses this principle to achieve rapid scanning imaging of biological samples without fluorescence labeling. This technology has high sensitivity, non-destructive, high spatial resolution and other advantages, and has been widely used in cell biology, tumor research, neuroscience research and other fields. The device for stimulated Raman detection mainly includes a femtosecond stimulated Raman spectrometer, a collinear multi-beam stimulated Raman scattering microscope and a lock-in amplifier. The femtosecond stimulated Raman spectrometer is a kind of nonlinear optical spectroscopy with the advantages of time-resolved vibrational spectroscopy, allowing additional tools for interpreting and assigning molecular dynamics and detecting "dark" molecular states.

[0003] In the prior art, the femtosecond stimulated Raman spectrometer is an advanced time-resolved vibrational spectroscopy technology used to study the structure dynamics of molecular excited states. However, during the detection of samples, factors such as temperature, humidity and vibration may have some impact on its performance. When using the femtosecond stimulated Raman spectrometer in humid weather in the south, the high humidity environment may cause water vapor to condense on the surface of the optical element, affecting the transmission of the optical path and the signal strength. At the same time, changes in humidity may change the physical and chemical properties of the sample. Especially for samples that are easily hygroscopic or susceptible to moisture, their Raman spectra may be significantly affected, which may affect the detection accuracy of the femtosecond stimulated Raman spectrometer. SUMMARY

[0004] In view of the deficiencies of the prior art, the present application provides a device and method for stimulated Raman detection to solve the problems raised in the background art.

[0005] The above technical purpose of the present application is achieved by the following technical scheme:

[0006] The utility model provides a device and method for stimulated raman detection, including femtosecond stimulated raman spectrometer body, the both sides of femtosecond stimulated raman spectrometer body are fixedly installed with support frame respectively, one side of support frame is provided with side plate, one side of side plate is provided with sheltering board, one side of side plate and sheltering board is opened with observation hole respectively, the inside fixed sleeve of observation hole has transparent board, temperature and humidity adjustment subassembly is arranged in one side of two side plates, temperature and humidity adjustment subassembly includes: two receiving holes, two receiving holes are opened in one side of two side plates respectively, the inside of receiving hole is provided with operating column, one side of operating column is fixedly installed with temperature -rising frame, the inside fixed installation of temperature -rising frame has heating wire, the inside fixed sleeve of temperature -rising frame has blocking net, one side of temperature -rising frame is fixedly installed with metal net, the bottom of operating column is fixedly installed with support frame, one side of the inside of support frame is opened with mounting hole, the inside fixed sleeve of mounting hole has micro -fan, one side of operating column is opened with air -out groove, the inside fixed sleeve of air -out groove has dustproof net, one side of operating column is opened with refrigeration hole, refrigeration hole is linked together with air -out groove, the inside fixed installation of refrigeration hole has semiconductor refrigeration piece, the inside fixed sleeve of refrigeration hole has heat dissipation net, and the hot end of semiconductor refrigeration piece faces heat dissipation net, one side of operating column is opened with exhaust hole, and exhaust hole is linked together with air -out groove, the inside fixed installation of exhaust hole has exhaust fan, one side of femtosecond stimulated raman spectrometer body is fixedly installed with PLC controller, and PLC controller is electrically connected with heating wire, micro -fan, semiconductor refrigeration piece and exhaust fan respectively, one side of operating column is provided with detection subassembly for detecting the temperature and humidity of femtosecond stimulated raman spectrometer body, the inside of receiving hole is provided with adjusting subassembly for moving operating column.

[0007] By adopting the above technical scheme, by setting the heating wire, first, the position of the operating column and the temperature raising frame is moved to the sample detection position of the femtosecond stimulated Raman spectrometer body by using the adjusting assembly, when the position of the operating column and the temperature raising frame is close to the sample table of the femtosecond stimulated Raman spectrometer body, by using the heating wire, the temperature of the heating wire is raised and the temperature inside the temperature raising frame is raised at the same time, and then the micro fan is started at the same time, after the wind power generated by the micro fan, the wind power blown by the micro fan will reach the position of the heating wire through the metal net, and then the hot air will be discharged through the blocking net and reach the sample detection position of the femtosecond stimulated Raman spectrometer body, so as to conveniently reduce the humidity around the detection position of the femtosecond stimulated Raman spectrometer body, prevent the surface of the optical element of the femtosecond stimulated Raman spectrometer body from condensing water vapor due to high humidity environment, affect the light path transmission and signal strength, and prevent the increase of humidity from increasing the water content in the sample, so as to change the molecular structure and vibration mode of the sample, and then affect the generation and transmission of the Raman signal, and then, by using the semiconductor refrigerating sheet, the refrigerating surface of the semiconductor refrigerating sheet faces the inside of the air outlet groove, and then after refrigeration, the cold air of the semiconductor refrigerating sheet is extracted by using the exhaust fan and is sprayed out through the dustproof net, so as to conveniently reduce the temperature of the sample detection position of the femtosecond stimulated Raman spectrometer body, prevent the temperature of the detection position from being too high to cause the molecular motion in the sample to be intensified, thereby increasing the frequency of the occurrence of inelastic scattering events, and then affecting the strength and line shape of the Raman signal.

[0008] Preferably, the detection assembly comprises a detection table fixedly installed on one side of the operating column, a fixing groove is formed on one side of the detection table, a temperature sensor is fixedly sleeved on the inner circular wall surface of the fixing groove, the temperature sensor is electrically connected with the PLC controller, an installation groove is formed on one side of the detection table, and a humidity sensor is fixedly sleeved on the inner circular wall surface of the installation groove.

[0009] By adopting the above technical scheme, by setting the temperature sensor, when the femtosecond stimulated Raman spectrometer body is used to detect the sample, the temperature of the detection environment of the femtosecond stimulated Raman spectrometer body can be detected by the temperature sensor during the preparation work, when the temperature is too high, the temperature sensor will transmit a signal to the PLC controller, and then the PLC controller will control the semiconductor refrigerating sheet and the exhaust fan to start, so as to reduce the temperature around the detection position of the femtosecond stimulated Raman spectrometer body, and at the same time, the humidity around the sample detection position of the femtosecond stimulated Raman spectrometer body can be detected by the humidity sensor, and then when the humidity is too high, the humidity sensor will transmit a signal to the PLC controller, and then the PLC controller will start the heating wire and the micro fan, so as to timely reduce the humidity around the detection position of the femtosecond stimulated Raman spectrometer body.

[0010] Preferably, the adjusting assembly comprises an adjusting column movably sleeved in the receiving hole, a limiting groove is formed on one side of the adjusting column, an electric push rod is fixedly sleeved in the limiting groove, a telescopic rod of the electric push rod is fixedly installed with the operation column, rotating columns are fixedly installed on the top surface and the bottom surface of the adjusting column respectively, rotating grooves and rotating holes are formed on the top surface and the bottom surface of the receiving hole respectively, the rotating column on the top of the adjusting column is movably sleeved with the rotating hole, the rotating column on the bottom of the adjusting column is movably sleeved with the rotating groove, a stepping motor is fixedly installed on the top surface of the side plate, and a driving shaft of the stepping motor is fixedly installed with the rotating column.

[0011] By adopting the above technical scheme, the adjusting column is arranged, the stepping motor is used, the driving shaft of the stepping motor rotates to drive the rotating column, the adjusting column, the operation column and the heating frame to rotate, so that the operation column and the heating frame can be moved out of the inside of the receiving hole, then the electric push rod is used, the telescopic rod of the electric push rod moves to push the operation column and the heating frame to move, so that the operation column, the heating frame and the detection table can be close to the sample detection position of the femtosecond stimulated Raman spectrometer body, thereby facilitating detection of the humidity and temperature around the sample detection position of the femtosecond stimulated Raman spectrometer body, so that the sample can be detected in a suitable environment.

[0012] Preferably, the top surface and one side of the side plate are fixedly installed with fixed blocks respectively, the positions of the two fixed blocks correspond to each other, a rotating column is fixedly installed on the side close to each other of the two fixed blocks, sliding grooves are formed on the top surface and the bottom surface of the shielding plate respectively, and the rotating column is movably sleeved with the sliding grooves.

[0013] By adopting the above technical scheme, when the environmental humidity and temperature have a great influence on sample detection, first, the initial positions of the side plate and the shielding plate are parallel, then the shielding plate is slid to make the rotating column reach one end inside the sliding groove, and then the shielding plate is rotated to make the side plate and the shielding plate present an L shape, at this time, the two shielding plates will be in front of the femtosecond stimulated Raman spectrometer body, thereby facilitating shielding of the sample detected by the femtosecond stimulated Raman spectrometer body.

[0014] Preferably, a supporting hole is formed on one side of the shielding plate, a reed clamping frame is fixedly installed in the inside of the supporting hole, a limiting frame is fixedly installed on one side of the side plate, a clamping column is fixedly installed in the inside of the limiting frame, and the clamping column is movably clamped with the reed clamping frame.

[0015] By adopting the above technical scheme, when the shielding plate is received in parallel with the side plate, one end of the shielding plate is pressed to make the clamping column clamped into the inside of the reed clamping frame, thereby facilitating limitation of the shielding plate.

[0016] Preferably, the inside of the support frame is fixedly provided with a guide column, one side of the support frame is provided with a blocking block, two clamping plates are fixedly arranged on one side of the blocking block, two clamping grooves are formed in one side of the support frame, the clamping plates are movably clamped with the clamping grooves, a support column is fixedly arranged on one side of the side plate, and a positioning hole is formed in one side of the support column and movably sleeved with the guide column.

[0017] By adopting the above technical scheme, the blocking block is moved to drive the clamping plate to move, the blocking block is moved away from one side of the support frame, then the side plate is moved to drive the support column to move, and the support column can be pulled out from the inside of the support frame, so that the side plate can be disassembled conveniently.

[0018] Preferably, the top surface of the support frame is provided with a dehumidification box, a partition net is fixedly arranged in the inside of the dehumidification box, a sealing groove is formed in the inner wall of the dehumidification box, a filter screen is movably sleeved in the inside of the dehumidification box, a clamping frame is fixedly sleeved on the outside of the filter screen, the clamping frame is movably clamped with the sealing groove, two clamping grooves are formed in the top surface of the support frame, two clamping blocks are fixedly arranged on the bottom surface of the dehumidification box, and the clamping blocks are movably clamped with the clamping grooves.

[0019] By adopting the above technical scheme, the dehumidification box is arranged, the dehumidification agent is put into the inside of the dehumidification box, and the humidity of the detection environment of the femtosecond stimulated Raman spectrometer body can be reduced in the severe humid weather in the south.

[0020] Preferably, a drainage hole is formed in one side of the dehumidification box, threads are formed in the inner circular wall surface of the drainage hole, a sealing plug is threadedly connected to the inner circular wall surface of the drainage hole, and threads are formed in the outer circular wall surface of the sealing plug.

[0021] By adopting the above technical scheme, the humidity water absorbed in the inside of the dehumidification box can be discharged.

[0022] The application also provides a method for stimulated Raman detection, and the specific steps are as follows.

[0023] S1: the rotating column, the adjusting column, the operating column and the temperature rising frame are rotated by using the stepping motor, so that the operating column and the temperature rising frame are moved out of the inside of the receiving hole, then the operating column and the temperature rising frame are moved by using the electric push rod, the operating column and the temperature rising frame are moved by the movement of the telescopic rod of the electric push rod, and the operating column, the temperature rising frame and the detection table are close to the sample detection position of the femtosecond stimulated Raman spectrometer body in the first step.

[0024] S2: When the femtosecond stimulated Raman spectrometer body is used to detect the sample before the preparation work, the temperature sensor can detect the temperature of the detection environment of the femtosecond stimulated Raman spectrometer body, when the temperature is too high, the temperature sensor will transmit the signal to the PLC controller, and then the PLC controller will control the semiconductor refrigerating sheet and the exhaust fan to start, so that the second step can be achieved to detect the temperature around the sample detection position of the femtosecond stimulated Raman spectrometer body;

[0025] S 3: The cold air of the semiconductor refrigerating sheet is extracted by using the exhaust fan and is sprayed out through the dust screen, so that the third step of reducing the temperature of the sample detection position of the femtosecond stimulated Raman spectrometer body is achieved, and the temperature of the detection position is prevented from being too high to cause the molecular motion in the sample to be intensified, so that the frequency of the occurrence of inelastic scattering events is increased, and then the intensity and line shape of the Raman signal are affected;

[0026] S 4: The humidity around the sample detection position of the femtosecond stimulated Raman spectrometer body can be detected by the humidity sensor, and then in the case that the humidity is too high, the humidity sensor will transmit the signal to the PLC controller, and then the PLC controller will start the heating wire and the micro fan, so that the fourth step of detecting the humidity around the sample detection position of the femtosecond stimulated Raman spectrometer body is achieved;

[0027] S 5: The temperature of the heating wire is increased, and then the micro fan is started at the same time, the wind power blown by the micro fan reaches the position of the heating wire through the metal screen, and then the hot air is discharged through the blocking screen and reaches the sample detection position of the femtosecond stimulated Raman spectrometer body, so that the fifth step of reducing the humidity around the detection position of the femtosecond stimulated Raman spectrometer body is achieved, and the increase of the humidity is prevented from causing the water content in the sample to be increased, so that the molecular structure and vibration mode of the sample are changed, and then the generation and transmission of the Raman signal are affected.

[0028] In summary, the present application mainly has the following beneficial effects:

[0029] The femtosecond stimulated Raman spectrometer body, the supporting frame, the side plate, the shielding plate, the observation hole, the transparent plate, the operation column, the heating frame, the heating wire, the blocking screen, the metal screen, the supporting frame and the micro fan are used in cooperation, the hot air is discharged through the blocking screen and reaches the sample detection position of the femtosecond stimulated Raman spectrometer body, so that the humidity around the detection position of the femtosecond stimulated Raman spectrometer body is reduced, the water vapor on the surface of the optical element of the femtosecond stimulated Raman spectrometer body is prevented from being condensed in the high humidity environment, the light path transmission and the signal intensity are affected, and the increase of the humidity is prevented from causing the water content in the sample to be increased, so that the molecular structure and vibration mode of the sample are changed, and then the generation and transmission of the Raman signal are affected.

[0030] By the cooperation of the operation column, the air outlet groove, the dust screen, the refrigeration hole, the semiconductor refrigeration sheet, the heat dissipation net, the air extraction hole and the air extraction fan, the temperature of the sample detection position of the femtosecond stimulated Raman spectrometer body can be reduced, the temperature of the detection position is prevented from being too high to cause the molecular movement in the sample to be intensified, the frequency of the occurrence of the inelastic scattering event is increased, and then the intensity and the line shape of the Raman signal are affected. BRIEF DESCRIPTION OF DRAWINGS

[0031] Figure 1 is a schematic diagram of the stereoscopic structure of the present application;

[0032] Figure 2 is a schematic diagram of the side plate structure of the present application;

[0033] Figure 3 is a schematic diagram of the shielding plate structure of the present application;

[0034] Figure 4 is a schematic diagram of the support frame structure of the present application;

[0035] Figure 5 is a schematic diagram of the transparent plate structure of the present application;

[0036] Figure 6 is a schematic diagram of the reed clamping frame structure of the present application;

[0037] Figure 7 is a schematic diagram of the support column structure of the present application;

[0038] Figure 8 is a schematic diagram of the operation column structure of the present application;

[0039] Figure 9 is a schematic diagram of the adjusting column structure of the present application;

[0040] Figure 10 is a schematic diagram of the dehumidification box structure of the present application.

[0041] Reference signs: 1, femtosecond stimulated Raman spectrometer body; 2, support frame; 3, side plate; 4, shielding plate; 5, observation hole; 6, transparent plate; 7, storage hole; 8, operation column; 9, warming frame; 10, heating wire; 11, blocking net; 12, metal net; 13, support frame; 14, mounting hole; 15, micro fan; 16, air outlet groove; 17, dustproof net; 18, refrigeration hole; 19, semiconductor refrigeration piece; 20, heat dissipation net; 21, air extraction hole; 22, air extraction fan; 23, detection table; 24, fixing groove; 25, temperature sensor; 26, mounting groove; 27, humidity sensor; 28, adjusting column; 29, limiting groove; 30, electric push rod; 31, rotating column; 32, rotating groove; 33, rotating hole; 34, stepping motor; 35, fixed block; 36, rotating column; 37, sliding groove; 38, support hole; 39, reed clamping frame; 40, limiting frame; 41, clamping column; 42, guide column; 43, blocking block; 44, clamping plate; 45, clamping groove; 46, support column; 47, positioning hole; 48, dehumidification box; 49, separation net; 50, sealing groove; 51, filter net; 52, clamping frame; 53, drainage hole; 54, sealing plug; 55, clamping groove; 56, clamping block. DETAILED DESCRIPTION

[0042] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor are within the scope of protection of the present application.

[0043] Example 1: Reference Figure 1 、 Figure 2 、 Figure 3 、 Figure 4 、 Figure 5 、 Figure 6 、 Figure 7 、 Figure 8 and Figure 9The utility model provides a device and method for stimulated raman detection, including femtosecond stimulated raman spectrometer body 1, and the both sides of femtosecond stimulated raman spectrometer body 1 are fixedly installed with support frame 2 respectively, one side of support frame 2 is provided with side plate 3, one side of side plate 3 is provided with baffle 4, and one side of side plate 3 and baffle 4 is opened with observation hole 5 respectively, and the inside of observation hole 5 is fixedly sleeved with transparent plate 6, one side of two side plates 3 is provided with temperature and humidity adjusting assembly, temperature and humidity adjusting assembly includes two receiving holes 7, and two receiving holes 7 are opened in one side of two side plates 3 respectively, and the inside of receiving hole 7 is provided with operating column 8, one side of operating column 8 is fixedly installed with temperature -rising frame 9, and heating wire 10 is fixedly installed in the inside of temperature -rising frame 9, and blocking net 11 is fixedly sleeved in the inside of temperature -rising frame 9, and one side of temperature -rising frame 9 is fixedly installed with metal net 12, and the bottom of operating column 8 is fixedly installed with support frame 13, and one side of the inside of support frame 13 is opened with mounting hole 14, and micro fan 15 is fixedly sleeved in the inside of mounting hole 14, and one side of operating column 8 is opened with air outlet groove 16, and dustproof net 17 is fixedly sleeved in the inside of air outlet groove 16, and one side of operating column 8 is opened with refrigeration hole 18, and refrigeration hole 18 is communicated with air outlet groove 16, and semiconductor refrigeration piece 19 is fixedly installed in the inside of refrigeration hole 18, and heat dissipation net 20 is fixedly sleeved in the inside of refrigeration hole 18, and the hot end of semiconductor refrigeration piece 19 faces heat dissipation net 20, and one side of operating column 8 is opened with exhaust hole 21, and exhaust hole 21 is communicated with air outlet groove 16, and exhaust fan 22 is fixedly installed in the inside of exhaust hole 21, and one side of femtosecond stimulated raman spectrometer body 1 is fixedly installed with PLC controller, and PLC controller is electrically connected with heating wire 10, micro fan 15, semiconductor refrigeration piece 19 and exhaust fan 22 respectively, and one side of operating column 8 is provided with detection assembly for detecting the temperature and humidity of femtosecond stimulated raman spectrometer body 1, and the inside of receiving hole 7 is provided with adjusting assembly for moving operating column 8, by setting heating wire 10, first by using adjusting assembly, the position of operating column 8 and temperature -rising frame 9 is moved sample detection position of femtosecond stimulated raman spectrometer body 1, when the position of operating column 8 and temperature -rising frame 9 is close to sample stage of femtosecond stimulated raman spectrometer body 1, by using heating wire 10, the temperature of heating wire 10 is raised and the temperature in the inside of temperature -rising frame 9 is raised simultaneously, then micro fan 15 is started simultaneously, after the wind power of micro fan 15 is generated, the wind power of micro fan 15 reaches the position of heating wire 10 through metal net 12, then hot air is discharged through blocking net 11 and reaches sample detection position of femtosecond stimulated raman spectrometer body 1, thereby the humidity around the detection position of femtosecond stimulated raman spectrometer body 1 is reduced, the optical element surface of femtosecond stimulated raman spectrometer body 1 is prevented from condensing water vapor in high humidity environment, the light path transmission and signal strength are influenced, the moisture content in sample is prevented from increasing due to the increase of humidity, thereby the molecular structure and vibration mode of sample are changed, then the generation and transmission of raman signal are influenced, then by using semiconductor refrigeration piece 19,The refrigeration surface of the semiconductor refrigeration sheet 19 faces the inside of the air outlet groove 16, and then after refrigeration, the cold air of the semiconductor refrigeration sheet 19 is extracted by using the air extraction fan 22 and is sprayed out through the dust screen 17, so as to conveniently reduce the temperature of the sample detection position of the femtosecond stimulated Raman spectrometer body 1, prevent the temperature of the detection position from being too high to cause the molecular motion in the sample to be intensified, thereby increasing the occurrence frequency of non-elastic scattering events, and then affecting the intensity and line shape of the Raman signal.

[0044] Example 2: Based on the above example 1, reference Figure 1 、 Figure 2 、 Figure 3 、 Figure 5 、 Figure 6 、 Figure 7 、 Figure 8 and Figure 9The detection assembly includes a detection table 23 fixedly installed on one side of the operation column 8, one side of the detection table 23 is provided with a fixed groove 24, the inner circular wall surface of the fixed groove 24 is fixedly sleeved with a temperature sensor 25, the temperature sensor 25 is electrically connected with the PLC controller, one side of the detection table 23 is provided with a mounting groove 26, the inner circular wall surface of the mounting groove 26 is fixedly sleeved with a humidity sensor 27, the humidity sensor 27 is electrically connected with the PLC controller, through the temperature sensor 25, when the femtosecond stimulated Raman spectrometer body 1 is used to detect the sample, the temperature in the detection environment of the femtosecond stimulated Raman spectrometer body 1 can be detected during the preparation work, when the temperature is too high, the temperature sensor 25 will transmit the signal to the PLC controller, and then the PLC controller will control the semiconductor refrigerating sheet 19 and the exhaust fan 22 to start, so as to reduce the temperature around the detection position of the femtosecond stimulated Raman spectrometer body 1, at the same time, the humidity sensor 27 can detect the humidity around the sample detection position of the femtosecond stimulated Raman spectrometer body 1, and then in the case that the humidity is too high, the humidity sensor 27 will transmit the signal to the PLC controller, and then the PLC controller will start the heating wire 10 and the micro fan 15, so as to timely reduce the humidity around the detection position of the femtosecond stimulated Raman spectrometer body 1, the adjusting assembly includes an adjusting column 28 movably sleeved in the receiving hole 7, one side of the adjusting column 28 is provided with a limiting groove 29, the limiting groove 29 is fixedly sleeved with an electric push rod 30, the telescopic rod of the electric push rod 30 is fixedly installed with the operation column 8, the top surface and the bottom surface of the adjusting column 28 are respectively fixedly installed with rotating columns 31, the inside top surface and the bottom surface of the receiving hole 7 are respectively provided with rotating grooves 32 and rotating holes 33, the rotating column 31 located at the top of the adjusting column 28 is movably sleeved with the rotating hole 33, the rotating column 31 located at the bottom of the adjusting column 28 is movably sleeved with the rotating groove 32, the top surface of the side plate 3 is fixedly installed with a stepping motor 34, the driving shaft of the stepping motor 34 is fixedly installed with the rotating column 31, through the adjusting column 28, through the use of the stepping motor 34, the rotation of the driving shaft of the stepping motor 34 will drive the rotating column 31, the adjusting column 28, the operation column 8 and the temperature rising frame 9 to rotate, and then the operation column 8 and the temperature rising frame 9 can be moved out of the inside of the receiving hole 7, and then through the use of the electric push rod 30, the movement of the telescopic rod of the electric push rod 30 will push the operation column 8 and the temperature rising frame 9 to move, and then the operation column 8, the temperature rising frame 9 and the detection table 23 can be close to the sample detection position of the femtosecond stimulated Raman spectrometer body 1, so as to facilitate the detection of the humidity and the temperature around the sample detection position of the femtosecond stimulated Raman spectrometer body 1, so that the sample can be detected in a suitable environment.

[0045] Example 3: based on the above examples 1 or 2, reference Figure 1 、 Figure 2 、 Figure 3 ,Figure 4 、 Figure 5 、 Figure 6 and Figure 7 The top surface and one side of the side plate 3 are respectively fixedly installed with fixed blocks 35, the positions of the two fixed blocks 35 correspond to each other, and one side of the two fixed blocks 35 is fixedly installed with a rotating column 36. The top surface and the bottom surface of the shielding plate 4 are respectively provided with sliding grooves 37, and the rotating column 36 is movably sleeved with the sliding grooves 37. When the environmental humidity and temperature have a great influence on the sample detection, the initial positions of the side plate 3 and the shielding plate 4 are parallel, then the sliding shielding plate 4 is moved to make the rotating column 36 reach the inside end of the sliding groove 37, and then the shielding plate 4 is rotated to make the side plate 3 and the shielding plate 4 form an L shape. At this time, the two shielding plates 4 will be blocked in front of the femtosecond stimulated Raman spectrometer body 1, so as to conveniently shield the sample detected by the femtosecond stimulated Raman spectrometer body 1. One side of the shielding plate 4 is provided with a supporting hole 38, a reed clamping frame 39 is fixedly installed in the inside of the supporting hole 38, one side of the side plate 3 is fixedly installed with a limiting frame 40, a clamping column 41 is fixedly installed in the inside of the limiting frame 40, and the clamping column 41 is movably clamped with the reed clamping frame 39. When the shielding plate 4 is stored in parallel with the position of the side plate 3, one end of the shielding plate 4 is pressed to make the clamping column 41 clamped in the inside of the reed clamping frame 39, so as to conveniently limit the shielding plate 4. A guide column 42 is fixedly installed in the inside of one side of the supporting frame 2, a blocking block 43 is arranged on one side of the supporting frame 2, two clamping plates 44 are fixedly installed on one side of the blocking block 43, two clamping grooves 45 are arranged on one side of the supporting frame 2, the clamping plates 44 are movably clamped with the clamping grooves 45, a supporting column 46 is fixedly installed on one side of the side plate 3, a positioning hole 47 is arranged on one side of the supporting column 46, and the positioning hole 47 is movably sleeved with the guide column 42. The blocking block 43 is moved to drive the clamping plate 44 to move, the blocking block 43 is moved away from one side of the supporting frame 2, then the side plate 3 is moved to drive the supporting column 46 to move, the supporting column 46 can be pulled out from the inside of the supporting frame 2, so as to conveniently disassemble the side plate 3.

[0046] Example 4: based on the above examples 1, 2 or 3, referring to Figure 1 、 Figure 2 、 Figure 4 and Figure 10The top surface of the support frame 2 is provided with a dehumidification box 48, a partition net 49 is fixedly installed in the inside of the dehumidification box 48, a sealing groove 50 is formed in the inner wall of the dehumidification box 48, a filter screen 51 is movably sleeved in the inside of the dehumidification box 48, a clamping frame 52 is fixedly sleeved outside the filter screen 51, two clamping grooves 55 are formed in the top surface of the support frame 2 when the clamping frame 52 is movably clamped with the sealing groove 50, two clamping blocks 56 are fixedly installed on the bottom surface of the dehumidification box 48, and the clamping blocks 56 are movably clamped with the clamping grooves 55. Through the dehumidification box 48, the dehumidification agent is put into the inside of the dehumidification box 48, so that the humidity of the detection environment of the femtosecond stimulated Raman spectrometer body 1 can be reduced in the heavy humid weather in the south. A drainage hole 53 is formed on one side of the dehumidification box 48, threads are formed on the inner circular wall surface of the drainage hole 53, a sealing plug 54 is threadedly connected to the inner circular wall surface of the drainage hole 53, and threads are formed on the outer circular wall surface of the sealing plug 54.

[0047] Based on the above-mentioned embodiments 1, 2, 3 or 4, with reference to Figures 1-10 The application also provides a method for stimulated Raman detection, and the specific steps are as follows:

[0048] S1: The operation column 8 and the temperature raising frame 9 are moved out of the inside of the storage hole 7 by rotating the rotating column 31, the adjusting column 28, the operation column 8 and the temperature raising frame 9 by using the stepper motor 34, and then the operation column 8 and the temperature raising frame 9 are moved by using the electric push rod 30, so that the operation column 8, the temperature raising frame 9 and the detection table 23 are close to the sample detection position of the femtosecond stimulated Raman spectrometer body 1 in the first step;

[0049] S2: Before the femtosecond stimulated Raman spectrometer body 1 is used to detect the sample, the temperature in the detection environment of the femtosecond stimulated Raman spectrometer body 1 is detected by using the temperature sensor 25 in the preparation work, the signal is transmitted to the PLC controller when the temperature is too high, and then the PLC controller controls the semiconductor refrigerating sheet 19 and the exhaust fan 22 to start, so that the temperature around the sample detection position of the femtosecond stimulated Raman spectrometer body 1 is detected in the second step;

[0050] S3: The cold air of the semiconductor refrigerating sheet 19 is sucked out by using the exhaust fan 22 and sprayed out through the dustproof net 17, so that the temperature of the sample detection position of the femtosecond stimulated Raman spectrometer body 1 is reduced in the third step, the temperature of the detection position is prevented from being too high to cause the molecular movement of the sample to be intensified, so that the frequency of the occurrence of the inelastic scattering event is increased, and then the intensity and the line shape of the Raman signal are affected;

[0051] S 4: The humidity sensor 27 can detect the humidity around the sample detection position of the femtosecond stimulated Raman spectrometer body 1, and in the case of excessive humidity, the humidity sensor 27 will transmit a signal to the PLC controller, and the PLC controller will start the heating wire 10 and the micro fan 15, which can achieve the fourth step to detect the humidity around the sample detection position of the femtosecond stimulated Raman spectrometer body 1;

[0052] S 5: By increasing the temperature of the heating wire 10, and simultaneously starting the micro fan 15, the wind force of the micro fan 15 will reach the position of the heating wire 10 through the metal mesh 12, and then the hot air will be discharged through the blocking net 11 and reach the sample detection position of the femtosecond stimulated Raman spectrometer body 1, which can achieve the fifth step to reduce the humidity around the detection position of the femtosecond stimulated Raman spectrometer body 1, prevent the increase of humidity from increasing the water content in the sample, thereby changing the molecular structure and vibration mode of the sample, and then affecting the generation and transmission of Raman signal.

[0053] Working principle: please refer to Figures 1-10 As shown, by setting the heating wire 10, first by using the adjusting assembly to move the position of the operating column 8 and the temperature rising frame 9 to the sample detection position of the femtosecond stimulated Raman spectrometer body 1, when the position of the operating column 8 and the temperature rising frame 9 is close to the sample stage of the femtosecond stimulated Raman spectrometer body 1, by using the heating wire 10, the temperature of the heating wire 10 is increased and the temperature inside the temperature rising frame 9 is increased, and then the micro fan 15 is started, after the micro fan 15 generates wind force, the wind force of the micro fan 15 will reach the position of the heating wire 10 through the metal mesh 12, and then the hot air will be discharged through the blocking net 11 and reach the sample detection position of the femtosecond stimulated Raman spectrometer body 1, thereby reducing the humidity around the detection position of the femtosecond stimulated Raman spectrometer body 1, preventing the condensation of water vapor on the surface of the optical element of the femtosecond stimulated Raman spectrometer body 1 in high humidity environment, affecting the transmission of light path and signal strength, and preventing the increase of humidity from increasing the water content in the sample, thereby changing the molecular structure and vibration mode of the sample, and then affecting the generation and transmission of Raman signal, and then by using the semiconductor refrigeration piece 19, the refrigeration surface of the semiconductor refrigeration piece 19 faces the inside of the air outlet groove 16, and then after refrigeration, the cold air of the semiconductor refrigeration piece 19 is extracted by using the exhaust fan 22, and is sprayed out through the dustproof net 17, thereby reducing the temperature of the sample detection position of the femtosecond stimulated Raman spectrometer body 1, preventing the increase of the molecular motion in the sample caused by the high temperature of the detection position, thereby increasing the frequency of non-elastic scattering events, and then affecting the strength and line shape of the Raman signal.

[0054] When the temperature sensor 25 detects that the temperature of the environment surrounding the femtosecond stimulated Raman spectrometer body 1 is too high, the temperature sensor 25 transmits a signal to the PLC controller, and the PLC controller controls the semiconductor refrigeration sheet 19 and the exhaust fan 22 to start, so as to reduce the temperature of the environment surrounding the femtosecond stimulated Raman spectrometer body 1. Meanwhile, the humidity sensor 27 can detect the humidity of the environment surrounding the sample detection position of the femtosecond stimulated Raman spectrometer body 1. When the humidity is too high, the humidity sensor 27 transmits a signal to the PLC controller, and the PLC controller controls the heating wire 10 and the micro fan 15 to start, so as to reduce the humidity of the environment surrounding the sample detection position of the femtosecond stimulated Raman spectrometer body 1 in time.

[0055] By using the adjusting column 28 and the stepping motor 34, the rotation of the driving shaft of the stepping motor 34 drives the rotating column 31, the adjusting column 28, the operating column 8 and the temperature raising frame 9 to rotate, so that the operating column 8 and the temperature raising frame 9 can be moved out of the inside of the receiving hole 7. Then, by using the electric push rod 30, the movement of the telescopic rod of the electric push rod 30 drives the operating column 8 and the temperature raising frame 9 to move, so that the operating column 8, the temperature raising frame 9 and the detection table 23 can be close to the sample detection position of the femtosecond stimulated Raman spectrometer body 1, thereby facilitating the detection of the humidity and temperature of the environment surrounding the sample detection position of the femtosecond stimulated Raman spectrometer body 1, so that the sample can be detected in a suitable environment.

[0056] When the humidity and temperature of the environment have a great influence on the sample detection, first, the initial positions of the side plate 3 and the shielding plate 4 are parallel, then the shielding plate 4 is slid to make the rotating column 36 reach the inside of the sliding groove 37, and then the shielding plate 4 is rotated to make the side plate 3 and the shielding plate 4 form an L shape. At this time, the two shielding plates 4 are shielded in front of the femtosecond stimulated Raman spectrometer body 1, thereby facilitating the shielding of the sample detected by the femtosecond stimulated Raman spectrometer body 1.

[0057] Although the embodiments of the present application have been shown and described, it can be understood by those skilled in the art that various changes, modifications, replacements and variations can be made to the embodiments without departing from the principles and spirits of the present application, and the scope of the present application is defined by the appended claims and their equivalents.

Claims

1. Apparatus for stimulated Raman detection, characterized in that, The utility model relates to a kind of femtosecond stimulated raman spectrometer, including: Femtosecond stimulated raman spectrometer body (1), both sides of the femtosecond stimulated raman spectrometer body (1) are fixedly installed with support frame (2), one side of the support frame (2) is provided with side plate (3), one side of the side plate (3) is provided with baffle (4), one side of the side plate (3) and the baffle (4) is respectively opened with observation hole (5), the inside of the observation hole (5) is fixedly sleeved with transparent plate (6); Temperature and humidity adjusting assembly, set in one side of two side plates (3), the temperature and humidity adjusting assembly includes: two storage holes (7), two storage holes (7) are respectively opened in one side of two side plates (3), the inside of the storage hole (7) is provided with operating column (8), one side of the operating column (8) is fixedly installed with temperature rising frame (9), the inside of the temperature rising frame (9) is fixedly installed with heating wire (10), the inside of the temperature rising frame (9) is fixedly sleeved with blocking net (11), one side of the temperature rising frame (9) is fixedly installed with metal net (12), the bottom surface of the operating column (8) is fixedly installed with support frame (13), one side of the inside of the support frame (13) is opened with mounting hole (14), the inside of the mounting hole (14) is fixedly sleeved with micro fan (15), one side of the operating column (8) is opened with air outlet groove (16), the inside of the air outlet groove (16) is fixedly sleeved with dustproof net (17), one side of the operating column (8) is opened with refrigeration hole (18), the refrigeration hole (18) is communicated with the air outlet groove (16), the inside of the refrigeration hole (18) is fixedly installed with semiconductor refrigeration piece (19), the inside of the refrigeration hole (18) is fixedly sleeved with heat dissipation net (20), the hot end of the semiconductor refrigeration piece (19) is towards the heat dissipation net (20), one side of the operating column (8) is opened with air suction hole (21), the air suction hole (21) is communicated with the air outlet groove (16), the inside of the air suction hole (21) is fixedly installed with air suction fan (22), one side of the femtosecond stimulated raman spectrometer body (1) is fixedly installed with PLC controller, the PLC controller is electrically connected with the heating wire (10), the micro fan (15), the semiconductor refrigeration piece (19) and the air suction fan (22) respectively; One side of the operating column (8) is provided with detection assembly for detecting temperature and humidity of the femtosecond stimulated raman spectrometer body (1); The inside of the storage hole (7) is provided with adjusting assembly for moving the operating column (8); The detection assembly comprises a detection table (23) fixedly installed on one side of the operation column (8), one side of the detection table (23) is provided with a fixed groove (24), the inner circular wall surface of the fixed groove (24) is fixedly sleeved with a temperature sensor (25), the temperature sensor (25) is electrically connected with the PLC controller, one side of the detection table (23) is provided with a mounting groove (26), the inner circular wall surface of the mounting groove (26) is fixedly sleeved with a humidity sensor (27), and the humidity sensor (27) is electrically connected with the PLC controller; The adjusting assembly comprises an adjusting column (28) movably sleeved in the receiving hole (7), one side of the adjusting column (28) is provided with a limiting groove (29), the limiting groove (29) is fixedly sleeved with an electric push rod (30) in the inside, the telescopic rod of the electric push rod (30) is fixedly installed with the operation column (8), the top surface and the bottom surface of the adjusting column (28) are respectively fixedly installed with rotating columns (31), the inside bottom surface and the top surface of the receiving hole (7) are respectively provided with rotating grooves (32) and rotating holes (33), the rotating column (31) located at the top of the adjusting column (28) is movably sleeved with the rotating hole (33), the rotating column (31) located at the bottom of the adjusting column (28) is movably sleeved with the rotating groove (32), and the top surface of the side plate (3) is fixedly installed with a stepping motor (34), the driving shaft of the stepping motor (34) is fixedly installed with the rotating column (31).

2. The device for stimulated Raman detection of claim 1, wherein: The top surface and one side of the side plate (3) are respectively fixedly installed with fixed blocks (35), the positions of the two fixed blocks (35) correspond to each other, one side of the two fixed blocks (35) close to each other is fixedly installed with a rotating column (36), the top surface and the bottom surface of the shielding plate (4) are respectively provided with sliding grooves (37), and the rotating column (36) is movably sleeved with the sliding grooves (37).

3. The device for stimulated Raman detection of claim 1, wherein: One side of the shielding plate (4) is provided with a supporting hole (38), the inside of the supporting hole (38) is fixedly installed with a reed clamping frame (39), one side of the side plate (3) is fixedly installed with a limiting frame (40), the inside of the limiting frame (40) is fixedly installed with a clamping column (41), and the clamping column (41) is movably clamped with the reed clamping frame (39).

4. The device for stimulated Raman detection of claim 1, wherein: One side of the supporting frame (2) is fixedly installed with a guide column (42), one side of the supporting frame (2) is provided with a blocking block (43), one side of the blocking block (43) is fixedly installed with two clamping plates (44), one side of the supporting frame (2) is provided with two clamping grooves (45), the clamping plates (44) are movably clamped with the clamping grooves (45), one side of the side plate (3) is fixedly installed with a supporting column (46), one side of the supporting column (46) is provided with a positioning hole (47), and the positioning hole (47) is movably sleeved with the guide column (42).

5. The device for stimulated Raman detection of claim 1, wherein: The top surface of the support frame (2) is provided with a dehumidification box (48), a partition net (49) is fixedly installed in the dehumidification box (48), a sealing groove (50) is formed in the inner wall of the dehumidification box (48), a filter screen (51) is movably sleeved in the dehumidification box (48), a clamping frame (52) is fixedly sleeved outside the filter screen (51), the clamping frame (52) is movably clamped with the sealing groove (50), two clamping grooves (55) are formed in the top surface of the support frame (2), and two clamping blocks (56) are fixedly installed on the bottom surface of the dehumidification box (48).

6. The device for stimulated Raman detection of claim 5, wherein: A drainage hole (53) is formed in one side of the dehumidification box (48), threads are formed in the inner circular wall surface of the drainage hole (53), and a sealing plug (54) is threadedly connected to the inner circular wall surface of the drainage hole (53).

7. Method for stimulated Raman detection using the device for stimulated Raman detection according to any one of claims 1 to 6, characterized in that, The method comprises the following steps: S1: The rotating column (31), the adjusting column (28), the operation column (8) and the temperature rising frame (9) are rotated by using the stepper motor (34), so that the operation column (8) and the temperature rising frame (9) are moved out of the inside of the storage hole (7), and then the operation column (8) and the temperature rising frame (9) are moved by using the electric push rod (30), so that the operation column (8), the temperature rising frame (9) and the detection table (23) are close to the sample detection position of the femtosecond stimulated Raman spectrometer body (1); S2: Before the femtosecond stimulated Raman spectrometer body (1) detects the sample, the temperature sensor (25) detects the temperature in the detection environment of the femtosecond stimulated Raman spectrometer body (1), and when the temperature is too high, the temperature sensor (25) transmits a signal to the PLC controller, and then the PLC controller controls the semiconductor refrigerating sheet (19) and the exhaust fan (22) to start; S3: The cold air of the semiconductor refrigerating sheet (19) is exhausted by using the exhaust fan (22) and is sprayed out through the dustproof net (17), so that the temperature of the sample detection position of the femtosecond stimulated Raman spectrometer body (1) is reduced, the temperature of the detection position is prevented from being too high to cause the molecular motion of the sample to be intensified, the frequency of occurrence of the inelastic scattering event is increased, and then the intensity and linearity of the Raman signal are affected; S4: The humidity around the sample detection position of the femtosecond stimulated Raman spectrometer body (1) is detected by the humidity sensor (27), and when the humidity is too high, the humidity sensor (27) transmits a signal to the PLC controller, and then the PLC controller starts the heating wire (10) and the micro fan (15). S5: through the temperature rise of the heating wire (10), and then simultaneously start the micro fan (15), the wind power of the micro fan (15) blows through the metal mesh (12) to the position of the heating wire (10), and then the hot air is discharged through the blocking net (11) and reaches the sample detection position of the femtosecond stimulated Raman spectrometer body (1), which is convenient for reducing the humidity around the detection position of the femtosecond stimulated Raman spectrometer body (1), preventing the increase of the moisture content in the sample caused by the increase of the humidity, so as to change the molecular structure and vibration mode of the sample, and then affect the generation and transmission of the Raman signal.

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