Pesticide composition containing pydiflumetofen and fludioxonil and application thereof

Abstract

The invention belongs to the technical field of pesticide disinfestation and sterilization, and discloses a pesticide composition containing pydiflumetofen and fludioxonil and application thereof, the pesticide composition comprises a bactericide and an insecticide, the bactericide is pydiflumetofen and fludioxonil, and the insecticide is thiamethoxam. The pesticide composition disclosed by the invention is reasonable in component, can prevent various diseases and insect pests of plants, has a remarkable synergistic effect after being applied once, and is labor-saving and time-saving.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticide insecticide and sterilization, and discloses a pesticide composition containing fludioxonil and fludioxonil and application thereof. Background Art

[0002] Fluopicolide is a new type of pyridine amide fungicide that interferes with the respiratory chain complex II by acting on succinate dehydrogenase to hinder energy synthesis. Fluopicolide has high efficiency and broad spectrum characteristics, and can prevent and treat a variety of diseases, such as sclerotinia, fusarium head blight, gray mold, brown spot, powdery mildew, leaf spot, etc. It is suitable for crops such as rapeseed, corn, and soybeans. Its mechanism of action is to inhibit the ubiquinone reduction reaction in the respiratory chain complex II, thereby affecting the tricarboxylic acid cycle and the respiration of the bacteria. Its structural formula is as follows:

[0003]

[0004] Fludioxonil belongs to the benzopyrrole fungicide class. It mainly inhibits the pathogen protein activating enzyme PK-Ⅲ which has a signaling effect in the process of osmotic sensing transmission, and thus shows a good control effect on a variety of pathogens. Due to its unique mechanism of action, it has no cross-resistance with existing fungicides and is a class of pyrrole fungicides with great development prospects. It is used for seed treatment to prevent and control seed bacteria and cross-resistance with other fungicides. Its structural formula is as follows:

[0005]

[0006] Thiamethoxam is a second-generation neonicotinoid insecticide with a unique structure and excellent insecticidal activity. Its chemical name is 3-(2-chloro-thiazole-5-methyl)-4-N-nitrosimide-1,3,5-diazine. Compared with the first-generation neonicotinoid insecticide imidacloprid, it has the advantages of higher systemicity, wider insecticidal spectrum, lower dosage, etc., and can interfere with the feeding behavior of pests. As a second-generation nicotinoid insecticide with a completely new structure, it has contact, stomach poison and systemic effects on a variety of pests. By utilizing the excellent systemic activity and conduction characteristics of thiamethoxam, it can be used for seed treatment to control leaf pests and underground pests while ensuring the safety of germination and growth. Its structural formula is as follows:

[0007]

[0008] With the promotion and application of green control technology for crop diseases and insect pests, seed coating has become an important measure to control crop diseases and insect pests in the seedling stage and promote crop growth and high yield. Compared with traditional spraying, seed coating can significantly reduce the number of pesticide applications and reduce drift pollution caused by spraying. Moreover, due to its relatively concealed application method, it has the advantages of significant control effect on target pests and long lasting effect, and has little effect on natural enemy insects. The inventors of the present invention found through indoor tests and field efficacy that mixing fungicides fluopicolide and fludioxonil with insecticide thiamethoxam in appropriate proportions to treat seeds has a significant synergistic effect on plant diseases and pests, and can effectively prevent and control diseases and pests with one application. Summary of the invention

[0009] Based on the above content, the purpose of the present invention is to provide a pesticide composition containing fludioxonil and fludioxonil, which has reasonable components and can prevent multiple plant diseases and insect pests. The pesticide composition can be applied once with significant synergistic effect, saving labor and time.

[0010] In order to achieve the above object, the present invention adopts the following technical scheme: a pesticide composition containing fluopicolide and fludioxonil, wherein the pesticide composition comprises a fungicide and an insecticide, wherein the fungicide is fluopicolide and fludioxonil, and the insecticide is thiamethoxam.

[0011] Furthermore, the mass ratio of fludioxonil to fludioxonil in the fungicide is 1:15 to 20:1, or any value within the above numerical range;

[0012] Furthermore, the mass ratio of the fungicide fludioxonil to fludioxonil is 1:15, 1:10, 1:5, 1:2, 2:1, 3:1, 9:1, 18:1, 20:1, or any value between the above values;

[0013] Furthermore, the mass ratio of fludioxonil to fludioxonil in the fungicide is 1:10 to 18:1, or any value within the above numerical range;

[0014] Furthermore, the mass ratio of fludioxonil to fludioxonil in the fungicide is 1:10, 1:5, 1:2, 2:1, 3:1, 9:1, 18:1, or any value between the above values;

[0015] Furthermore, the mass ratio of fludioxonil to fludioxonil in the fungicide is 1:5 to 9:1, or any value within the above numerical range;

[0016] Furthermore, the mass ratio of fludioxonil to fludioxonil in the fungicide is 1:5, 1:2, 2:1, 3:1, 9:1, or any value between the above values;

[0017] Furthermore, the mass ratio of the fungicide to the insecticide is 3:2 to 3:25, or any value within the above numerical range;

[0018] Furthermore, the mass ratio of the fungicide to the insecticide is 3:2, 3:4, 1:2, 3:8, 3:10, 3:12, 3:14, 3:16, 4:25, 3:25, or any value between the above values;

[0019] Furthermore, the mass ratio of the fungicide to the insecticide is 3:4 to 3:16, or any value within the above numerical range;

[0020] Furthermore, the mass ratio of the fungicide to the insecticide is 3:4, 1:2, 3:8, 3:10, 3:12, 3:14, 3:16, or any value within the above numerical ranges;

[0021] Furthermore, the mass ratio of the fungicide to the insecticide is 3:4 to 3:14, or any value within the above numerical range;

[0022] Furthermore, the mass ratio of the fungicide to the insecticide is 3:4, 1:2, 3:8, 3:10, 3:12, 3:14, or any value within the above numerical ranges;

[0023] Furthermore, based on the total weight of the pesticide composition being 100 wt%, the total content of the fungicide in the pesticide composition is 0.5% to 50%;

[0024] Furthermore, based on the total weight of the pesticide composition being 100 wt%, the total content of the fungicide in the pesticide composition is 1% to 10%;

[0025] Furthermore, based on the total weight of the pesticide composition being 100 wt%, the total content of the fungicide in the pesticide composition is 2% to 8%.

[0026] Furthermore, based on the total weight of the pesticide composition being 100 wt%, the content of the insecticide in the pesticide composition is 5% to 50%;

[0027] Furthermore, based on the total weight of the pesticide composition being 100 wt%, the content of the insecticide in the pesticide composition is 10% to 40%;

[0028] Furthermore, based on 100 wt% of the total weight of the pesticide composition, the content of the insecticide in the pesticide composition is 15% to 35%.

[0029] Furthermore, the pesticide composition further comprises an adjuvant, and the adjuvant is selected from one or more of a wetting agent, a dispersant, an emulsifier, a thickener, a disintegrant, an antifreeze agent, a defoaming agent, a solvent, a preservative, a stabilizer, a warning color, a film former, a synergist and a carrier;

[0030] The wetting agent is selected from one or more of alkylbenzene sulfonate, alkylnaphthalene sulfonate, lignin sulfonate, sodium dodecyl sulfate, sodium dioctyl succinate sulfonate, alpha olefin sulfonate, alkylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, alkylphenol ethoxylate, fatty alcohol ethoxylate, fatty alcohol polyoxyethylene ether sodium sulfate, silkworm feces, saponin powder, soapberry powder, SOPA, detergent, emulsifier 2000 series and wetting penetrant F; and / or

[0031] The dispersant is selected from one or more of lignin sulfonate, alkylnaphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, tristyrylphenol ethoxylate phosphate, fatty alcohol ethoxylate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether methyl ether condensate sulfate, fatty amine polyoxyethylene ether, glycerol fatty acid ester polyoxyethylene ether, polycarboxylates, polyacrylic acid, phosphates, EO-PO block copolymers and EO-PO graft copolymers; and / or

[0032] The emulsifier is selected from one or more of calcium dodecylbenzene sulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenylethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrylphenol polyoxyethylene ether, castor oil polyoxyethylene ether and alkylphenol ether phosphate; and / or

[0033] The thickener is selected from one or more of xanthan gum, organic bentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose and white carbon black; and / or

[0034] The disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid and tartaric acid; and / or

[0035] The antifreeze agent is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons and inorganic salts; and / or

[0036] The defoaming agent is selected from C 10 -C 20 Saturated fatty acid compounds, silicone oil, silicone compounds, C8-C 10 One or more of fatty alcohols; and / or

[0037] The solvent is selected from one or more of benzene, toluene, xylene, durene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, alkylene carbonate, diesel, solvent oil, vegetable oil, vegetable oil derivatives and water; and / or

[0038] The preservative is selected from one or more of propionic acid, sodium propionic acid, sorbic acid, sodium sorbic acid, potassium sorbic acid, benzoic acid, sodium benzoic acid, sodium p-hydroxybenzoic acid, methyl p-hydroxybenzoate, kasone and 1,2-benzisothiazolin-3-one; and / or

[0039] The stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, white carbon black, talc, montmorillonite and starch; and / or

[0040] The warning color is selected from any one or more adjustment colors of blue, green, red, and purple; and / or

[0041] The film-forming agent is selected from one or more of sodium carboxymethyl starch, cellulose derivatives (sodium carboxymethyl cellulose, sodium alginate, polyvinyl alcohol), polyacrylic acid; and / or

[0042] Furthermore, the film-forming agent is selected from seed coating film-forming agent 851, seed coating film-forming agent 805; and / or

[0043] The synergist is selected from synergist phosphite, synergist ether; and / or

[0044] The carrier is selected from one or more of ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils and vegetable oil derivatives;

[0045] Furthermore, the pesticide composition is in the form of any one of a seed treatment suspension, a microcapsule suspension, and a dry seed dressing;

[0046] Furthermore, the pesticide composition is in the form of a seed treatment suspension.

[0047] The invention also discloses the application of the above pesticide composition in preventing and controlling plant diseases and / or insect pests.

[0048] Furthermore, the plants include corn, wheat, soybean, rice, peanut and / or cotton;

[0049] Furthermore, the plant is peanut, corn or wheat;

[0050] Further, the plant is peanut;

[0051] Furthermore, the plant is peanut, and the peanut disease is any one of peanut black spot, peanut brown spot, peanut web spot, peanut anthracnose, peanut scorch spot, peanut purple feather disease, flower rust, peanut bacterial wilt, peanut stem rot, peanut white rot, peanut root rot, peanut yellow mosaic virus, peanut stripe virus, peanut dwarf virus, and peanut crown rot;

[0052] Furthermore, the peanut disease is peanut white rot or peanut root rot;

[0053] The pathogen of peanut white rot is Sclerotium rolfsii, which belongs to the Ascomycetes. The mycelium of the pathogen is white, and the regular roots to dozens of roots are twisted into a thread shape and extend radially. The appearance is like white silk, so it is commonly known as white rot. Peanut white rot mainly harms the stems, fruit stalks and pods of peanuts. In the early stage of the disease, the diseased part turns brown and soft rots, and wavy spots appear on it. When the soil is moist and hidden, a layer of white mycelium is also covered on the plant residues and organic matter on the soil surface around the diseased plants. Many spherical sclerotia are formed in the mycelium. The tissue at the base of the affected stem rots, the cortex falls off, and fibrous tissue remains. The leaves of the diseased plants turn yellow, the edges are scorched, and finally wither and die. The affected fruit stalks and pods grow a lot of white mycelium, which rots in a wet rot state.

[0054] The peanut root rot is mainly caused by infection by a variety of Fusarium, including Fusarium oxysporum, Fusarium solani solani, Fusarium roseum, Fusarium tricinctum and Fusarium moniliforme. Peanut root rot can occur in all growth stages and mainly harms the roots of the plants. The pathogen infects the newly germinated seeds, causing them to rot; when the seedlings are affected, the taproot turns brown and the plants wither. When the adult plants are affected, sunken long brown spots appear on the taproot rhizomes, the root ends are wet and rotten, the cortex turns brown and rots, and is easy to detach and fall off, with no or very few lateral roots, shaped like a rat's tail. When it is humid, adventitious roots grow on the rhizomes. The aboveground part of the diseased plants is short, grows poorly, the leaves turn yellow, and there are few flowers and fruits, and most of them are barren fruits.

[0055] The peanut pest is any one of white grubs, peanut aphids, cotton bollworms, beet armyworms, armyworms, and new black spider scales.

[0056] Furthermore, the peanut pests are white grubs;

[0057] Furthermore, the grubs include Holotrichia oblita, Holotrichia parallela, Anomala corpulenta, Proagopertha lucidula, Anomala exoleta, Holotrichia trichophora and Maladera orientalis.

[0058] In order to obtain the desired insecticidal effect, the dosage of the pesticide composition varies depending on various factors, such as the crop to be protected, the type of pest, the degree of infection, climatic conditions, application site, application method, dosage form used, etc.

[0059] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0060] 1) The pesticide composition of the present invention can prevent both plant diseases and insect pests, has a long lasting effect, and has significant prevention effect after one application;

[0061] 2) The pesticide composition of the present invention has reasonable components and exhibits a certain synergistic effect on the prevention and treatment of pests and diseases within a certain range, which can reduce the amount of active ingredients used in pesticides and improve the prevention and treatment effect. DETAILED DESCRIPTION

[0062] In order to make the technical solutions, objectives and advantages of the present invention more clearly understood, the present invention is described with the following specific embodiments, but the present invention can be implemented in various forms and should not be limited by the embodiments described herein.

[0063] Preparation Example:

[0064] Preparation process of seed treatment suspension: according to the ratio, the active ingredients, adjuvants and water are mixed and stirred evenly through high shear, and then sanded by a sand grinder for 2.5 hours to make the average particle size reach 1-5 microns. Finally, the thickener, preservative and film-forming agent are added and continued to be sheared and stirred evenly to obtain the seed treatment suspension.

[0065] Preparation Example 1: 32.5% Fluopyraclostrobin·Fludioxonil·Thiamethoxam seed treatment suspension (5%+2.5%+25%)

[0066] Calculated by weight percentage, 5% fluazifop-hydroxyamide, 2.5% fludioxonil, 25% thiamethoxam, 4% triphenol polyoxyethylene ether, 5% fatty alcohol polyoxyethylene ether sulfate, 2% sodium lignin sulfonate, 1% polyacrylic acid emulsion, 0.25% xanthan gum, 6% rose red pigment, 5% propylene glycol, 1% magnesium aluminum silicate, 0.5% silicone defoamer, 1% sodium benzoate, and deionized water to make up the balance.

[0067] Preparation Example 2: 27.5% Fluopyraclostrobin·Fludioxonil·Thiamethoxam seed treatment suspension (5%+2.5%+20%)

[0068] Calculated by weight percentage, 5% fluazifop-butyl hydroxylamide, 2.5% fludioxonil, 20% thiamethoxam, 2% tristyrylphenol polyoxyethylene ether polyoxypropylene ether, 4% alkylphenol polyoxyethylene ether phosphate salt, 1% polycarboxylic acid sodium salt, 3% sodium carboxymethyl cellulose, 0.25% xanthan gum, 5% rose red pigment, 1% magnesium aluminum silicate, 0.5% silicone defoamer, 1% sodium sorbate, 1% polyacrylic acid emulsion, 5% propylene glycol, and deionized water make up the balance.

[0069] Preparation Example 3: 37.5% Fluopyraclostrobin·Fludioxonil·Thiamethoxam seed treatment suspension (5%+2.5%+30%)

[0070] Calculated by weight percentage, 5% fluazifop-hydroxylamide, 2.5% fludioxonil, 30% thiamethoxam, 2% fatty alcohol ethylene oxide-alkylene oxide copolymer, 3% phenethylphenol polyoxyethylene phosphate, 1% sodium lignin sulfonate, 4% polyoxyethylene sorbitan monooleate, 1% polyacrylic acid emulsion, 0.25% xanthan gum, 6% rose red pigment, 5% propylene glycol, 1% magnesium aluminum silicate, 0.5% silicone defoamer, 1% methyl paraben, and deionized water to make up the balance.

[0071] Preparation Example 4: 29% Fluopyraclostrobin Hydroxylamine·Fludioxonil·Thiamethoxam Seed Treatment Suspension Concentrate (3%+1%+25%)

[0072] Calculated by weight percentage, 3% fluazifop-hydroxyamide, 1% fludioxonil, 25% thiamethoxam, 3% isotridecyl alcohol polyoxyethylene ether, 3% fatty alcohol polyoxyethylene ether sulfate, 2% sodium lignin sulfonate, 2% polyacrylic acid emulsion, 0.25% xanthan gum, 7% rose red pigment, 5% propylene glycol, 1% magnesium aluminum silicate, 0.5% silicone defoamer, 1% sodium p-hydroxybenzoate, and deionized water to make up the balance.

[0073] Preparation Example 5: 28% Fluopyraclostrobin·Fludioxonil·Thiamethoxam seed treatment suspension (1%+2%+25%)

[0074] Calculated by weight percentage, 1% fluazifop-butyl hydroxylamide, 2% fludioxonil, 25% thiamethoxam, 2% ethylene glycol oxyethylene polyoxypropylene ether, 4% alkylphenol polyoxyethylene ether phosphate salt, 1% polycarboxylic acid sodium salt, 1% polyacrylic acid emulsion, 0.25% xanthan gum, 6% rose red pigment, 5% propylene glycol, 1% magnesium aluminum silicate, 0.5% silicone defoamer, 2% 1,2-benzisothiazolin-3-one, and deionized water to make up the balance.

[0075] Control Example 1: 7.5% Fluopyraclostrobin hydroxylamine·fludioxonil seed treatment suspension (5%+2.5%)

[0076] Calculated by weight percentage, 5% fluazifop-butyl hydroxylamide, 2.5% fludioxonil, 3% sorbitan oleate polyoxyethylene ether, 3% naphthalenesulfonate formaldehyde condensate, 2% sodium dodecyl sulfate, 3% tristyrylphenol polyoxyethylene ether phosphate, 1% polyacrylic acid emulsion, 0.3% xanthan gum, 5% rose red pigment, 5% propylene glycol, 1% magnesium aluminum silicate, 0.5% silicone defoamer, 1% sodium sorbate, and deionized water to make up the balance.

[0077] Control Example 2: 30% Fluopyraclostrobin Hydroxylamine·Thiamethoxam Seed Treatment Suspension Concentrate (5%+25%)

[0078] Calculated by weight percentage, 5% fluopicolide, 25% thiamethoxam, 3% phenethylphenol polyoxyethylene polyoxypropylene ether, 3% polyoxyethylene sorbitan monooleate, 3% fatty alcohol polyoxyethylene ether phosphate, 2% naphthalenesulfonate formaldehyde condensate, 1.5% polyacrylic acid emulsion, 0.2% xanthan gum, 4% rose red pigment, 5% ethylene glycol, 1% magnesium aluminum silicate, 0.5% silicone defoamer, 0.5% benzisothiazolinone potassium, and deionized water to make up the balance.

[0079] Comparative Example 3: 27.5% Fludioxonil·Thiamethoxam seed treatment suspension (2.5%+25%)

[0080] Calculated by weight percentage, 2.5% fludioxonil, 25% thiamethoxam, 2% glycerol fatty acid ester polyoxyethylene ether, 2% EO / PO block copolymer, 4% styrene phenol polyoxyethylene ether sulfate, 1% polyacrylic acid emulsion, 0.2% xanthan gum, 6% rose red pigment, 5% propylene glycol, 1% magnesium aluminum silicate, 0.4% silicone defoamer, 2% sodium benzoate, and deionized water to make up the balance.

[0081] Example 1: Indoor toxicity determination of peanut white rot

[0082] Test pathogen: Sclerotium rolfsii Sacc.

[0083] Experimental drugs: Fluoxetine hydroxylamide, fludioxonil, and thiamethoxam technical drugs.

[0084] Preparation of drugs: After dissolving the above original drugs in appropriate solvents, dilute them with 0.1% Tween 80 aqueous solution, prepare single-dose mother liquors respectively, and design different ratios according to the purpose of mixing and the activity of the drugs. Each single dose and each group of mixed drugs are prepared into the required series of mass concentrations. Under aseptic operation conditions, according to the test treatment, the pre-melted and sterilized PDA culture medium is quantitatively added to the sterile conical flask, and 10mL of each treatment solution is quantitatively drawn from low concentration to high concentration, and added to the above conical flasks respectively, shaken well, and then poured into the culture dish in equal amounts to prepare drug-containing plates of corresponding concentrations. The best synergistic ratio of fluazifop-butyl hydroxylamine and fludioxonil for the whole sclerotium is screened out, and the mixture of the above ratio is used as a fungicide combination, and is compounded with thiamethoxam in different mass ratios. The experiment sets a 0.1% Tween 80 aqueous solution without adding drugs as a blank control, and each treatment is repeated 4 times.

[0085] Cut the pre-cultured pathogens from the edge of the colony with a sterile borer under sterile conditions, inoculate the cake into the center of the drug-containing plate with an inoculator, cover the plate with a lid, and place it in a constant temperature incubator at 25±1℃ for culture.

[0086] Data statistics and analysis: Investigate the growth of pathogenic fungi hyphae based on the growth of bacteria in the blank control culture dish. Use a caliper to measure the diameter of the colony in millimeters (mm). Use the cross method to measure the diameter of each colony vertically once and take the average value.

[0087] According to the survey results, the mycelial growth inhibition rate of each treatment concentration on the target bacteria was calculated. The unit is percentage (%) and the calculation result is retained to two decimal places.

[0088] D=D1-D2

[0089] Where:

[0090] D——colony growth diameter;

[0091] D1——colony diameter;

[0092] D2——diameter of mushroom cake.

[0093]

[0094] Where:

[0095] I——hyphae growth inhibition rate;

[0096] D0——blank control colony growth diameter;

[0097] D T ——The growth diameter of the colony after treatment with chemicals.

[0098] The logarithmic value of each drug concentration and the corresponding probability value of mycelial growth inhibition rate were used for regression analysis. The EC of the toxicity regression line was calculated. 50 Values ​​and correlation coefficients.

[0099] Sun Yunpei method: The synergistic effect of mixed drugs is evaluated based on the co-toxicity coefficient (CTC). A co-toxicity coefficient of ≥120 indicates a synergistic effect; CTC≤80 indicates an antagonistic effect; and 80<CTC<120 indicates an additive effect.

[0100] Calculation of the co-toxicity coefficient (CTC value) of the mixture:

[0101]

[0102] Where:

[0103] ATI——Actual Toxicity Index of Mixture;

[0104] S——EC of standard fungicide 50 , the unit is milligrams per liter (mg / L);

[0105] M——EC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0106] TTI=TI A ×P A +TI B ×P B

[0107] Where:

[0108] TTI – Theoretical Toxicity Index of Mixtures;

[0109] TI A ——A: toxicity index of the agent;

[0110] P A ——The percentage of agent A in the mixture, in percentage (%);

[0111] TI B ——Toxicity index of agent B;

[0112] P B ——The percentage of agent B in the mixture, in percentage (%).

[0113]

[0114] Where:

[0115] CTC – Co-toxicity coefficient;

[0116] ATI——Actual Toxicity Index of Mixture;

[0117] TTI - Theoretical Toxicity Index of Mixture.

[0118] The test results are shown in the following table:

[0119] Table 1 Indoor bioassay results of fludioxonil, fludioxonil and their mixed agents against Sclerotium rolfsii

[0120]

[0121] There are differences in the mechanism of action of fluopicolide and fludioxonil. Their combined use is beneficial to overcome or delay the development of drug resistance and improve the control effect. From the indoor test results in Table 1, it can be seen that the combination of fluopicolide and fludioxonil has a good control effect on peanut white rot fungus. 50 The EC value of fludioxonil against peanut white rot is 2.1158 mg / L. 50 The ratio of fluopicolide to fludioxonil was 0.5047 mg / L. The ratio of fluopicolide to fludioxonil was 1:10-18:1, and the co-toxicity coefficient of fluopicolide to fludioxonil was 1:5-9:1, which was greater than 130, and the synergistic effect was obvious. When the mass ratio was 1:15 and 20:1, an additive effect was shown. When the mass ratio of the two was 2:1, the co-toxicity coefficient against peanut Sclerotium rolfsii was 184.397, and the synergistic effect was the most significant.

[0122] Table 2 Results of indoor toxicity test of fluopicolide + fludioxonil mixture and its mixture with thiamethoxam against peanut Sclerotium rolfsii

[0123] Test drug Virulence regression equation <![CDATA[EC 50 (mg.L -1 )]]> Co-toxicity coefficient (CTC) Fluoxathiapiprolin: Fludioxonil (2:1) y=1.561x+5.4274 0.5323 / Thiamethoxam / / / Thiamethoxam: fludioxonil: fludioxonil = 5:5:2.5 y=1.6560x+5.5141 0.4892 108.810 Thiamethoxam: fludioxonil: fludioxonil = 8:5:2.5 y=1.4985x+5.5136 0.4542 117.195 Thiamethoxam: fludioxonil: fludioxonil = 10:5:2.5 y=1.5392x+5.5726 0.4246 125.365 Thiamethoxam: fludioxonil: fludioxonil = 15:5:2.5 y=1.4865x+5.5816 0.4062 131.044 Thiamethoxam: fludioxonil: fludioxonil = 20:5:2.5 y=1.5116x+5.6279 0.3843 138.512 Thiamethoxam: fludioxonil: fludioxonil = 25:5:2.5 y=1.3872x+5.6046 0.3666 145.199 Thiamethoxam: fludioxonil: fludioxonil = 30:5:2.5 y=1.4778x+5.5745 0.4085 130.306 Thiamethoxam: fludioxonil: fludioxonil = 35:5:2.5 y=1.3996x+5.5217 0.4239 125.572 Thiamethoxam: fludioxonil: fludioxonil = 40:5:2.5 y=1.4153x+5.4148 0.5092 104.537 Thiamethoxam: fludioxonil: fludioxonil = 45:5:2.5 y=1.3529x+5.363 0.5391 98.739

[0124] Note: Thiamethoxam had no significant inhibitory activity against the pathogen causing peanut white rot at the designed highest concentration of 200 mg / L, and EC could not be calculated. 50 and virulence regression equations.

[0125] Thiamethoxam is a broad-spectrum insecticide, but it has no obvious activity against peanut Sclerotium rolfsii. The indoor test results in Table 2 show that the combination of the mixture of "flutrifloxystrobin + fludioxonil" with a mass ratio of 5:2.5 (2:1) and thiamethoxam showed a certain inhibitory effect on the growth of peanut Sclerotium rolfsii; among them, the mass ratio of thiamethoxam:flutrifloxystrobin:fludioxonil (10-35):5:2.5 showed a synergistic effect on peanut Sclerotium rolfsii, while the mass ratios of thiamethoxam:flutrifloxystrobin:fludioxonil (40-45):5:2.5 and (5-8):5:2.5) showed an additive effect on peanut Sclerotium rolfsii.

[0126] Indoor Example 2: Indoor Toxicity Determination of Peanut Root Rot

[0127] Test pathogen: Fusarium oxysporum.

[0128] Experimental drugs: Fluoxetine hydroxylamide, fludioxonil, and thiamethoxam technical drugs.

[0129] Preparation of the drug: After dissolving the above original drugs in appropriate solvents, dilute them with 0.1% Tween 80 aqueous solution, prepare single-dose mother liquors respectively, and design different ratios according to the purpose of mixing and the activity of the drug. Each single dose and each group of mixed drugs are prepared into the required series of mass concentrations. Under aseptic operation conditions, according to the test treatment, the pre-melted and sterilized PDA culture medium is quantitatively added to the sterile conical flask, and 10mL of each treatment solution is quantitatively drawn from low concentration to high concentration, and added to the above conical flask respectively, shaken well, and then poured into the culture dish in equal amounts to prepare drug-containing plates of corresponding concentrations. The best synergistic ratio of fluazifop-butyl hydroxylamine and fludioxonil for peanut root rot was screened out, and the mixture of the above ratio was used as a fungicide combination, and was compounded with thiamethoxam in different mass ratios. The experiment set up a 0.1% Tween 80 aqueous solution without adding drugs as a blank control, and each treatment was repeated 4 times.

[0130] Cut the pre-cultured pathogens from the edge of the colony with a sterile puncher under sterile conditions, inoculate the cake into the center of the drug-containing plate with an inoculator, cover the plate with a lid, and place it in a constant temperature incubator at 28°C for culture.

[0131] Data statistics and analysis: Investigate the growth of pathogenic fungi hyphae based on the growth of bacteria in the blank control culture dish. Use a caliper to measure the diameter of the colony in millimeters (mm). Use the cross method to measure the diameter of each colony vertically once and take the average value.

[0132] According to the survey results, the mycelial growth inhibition rate of each treatment concentration on the target bacteria was calculated. The unit is percentage (%) and the calculation result is retained to two decimal places.

[0133] D=D1-D2

[0134] Where:

[0135] D——colony growth diameter;

[0136] D1——colony diameter;

[0137] D2——diameter of mushroom cake.

[0138]

[0139] Where:

[0140] I——hyphae growth inhibition rate;

[0141] D0——blank control colony growth diameter;

[0142] D T ——The growth diameter of the colony after treatment with chemicals.

[0143] The logarithmic value of each drug concentration and the corresponding probability value of mycelial growth inhibition rate were used for regression analysis. The EC of the toxicity regression line was calculated. 50 Values ​​and correlation coefficients.

[0144] Sun Yunpei method: The synergistic effect of mixed drugs is evaluated based on the co-toxicity coefficient (CTC). A co-toxicity coefficient of ≥120 indicates a synergistic effect; CTC≤80 indicates an antagonistic effect; and 80<CTC<120 indicates an additive effect.

[0145] Calculation of the co-toxicity coefficient (CTC value) of the mixture:

[0146]

[0147] Where:

[0148] ATI——Actual Toxicity Index of Mixture;

[0149] S——EC of standard fungicide 50 , the unit is milligrams per liter (mg / L);

[0150] M——EC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0151] TTI=TI A ×P A +TI B ×P B

[0152] Where:

[0153] TTI – Theoretical Toxicity Index of Mixtures;

[0154] TI A ——A: toxicity index of the agent;

[0155] P A ——The percentage of agent A in the mixture, in percentage (%);

[0156] TI B ——Toxicity index of agent B;

[0157] P B ——The percentage of agent B in the mixture, in percentage (%).

[0158]

[0159] Where:

[0160] CTC – Co-toxicity coefficient;

[0161] ATI——Actual Toxicity Index of Mixture;

[0162] TTI - Theoretical Toxicity Index of Mixture.

[0163] The test results are shown in the following table:

[0164] Table 3 Results of indoor bioassay of fluazifop-butyl, fludioxonil and their mixed agents against Fusarium oxysporum

[0165]

[0166] There are differences in the mechanism of action of fluopicolide and fludioxonil. The combined use of fluopicolide and fludioxonil is beneficial to overcome or delay the development of drug resistance and improve the control effect. The test results show that, as shown in Table 3, the combination of fluopicolide and fludioxonil has a good control effect on the mycelial growth of Fusarium oxysporum. Fluopicolide and fludioxonil 1:15-20:1 both show synergistic effects; the co-toxicity coefficient of fluopicolide and fludioxonil 1:10-18:1 on Fusarium oxysporum is greater than 130, and the synergistic effect is obvious; among them, the ratio of fluopicolide: fludioxonil = 2:1 has better activity, and the co-toxicity coefficient is 197.651.

[0167] Table 4 Results of indoor toxicity test of fludioxonil + fludioxonil mixture and its mixture with thiamethoxam against Fusarium oxysporum

[0168]

[0169]

[0170] Note: Thiamethoxam had no significant inhibitory activity against the pathogen causing peanut root rot (Fusarium oxysporum) at the designed maximum concentration of 200 mg / L, and the EC could not be calculated. 50 and virulence regression equations.

[0171] Thiamethoxam is a broad-spectrum insecticide, but it has no obvious activity against peanut root rot fungi. The indoor test results in Table 4 show that the combination of the mixture of "fluoxetine + fludioxonil" with a mass ratio of 5:2.5 (2:1) and thiamethoxam showed a certain inhibitory effect on the growth of Fusarium oxysporum; among them, the mass ratio of thiamethoxam:fluoxetine:fludioxonil (15-40):5:2.5 showed a synergistic effect on Fusarium oxysporum, while the mass ratios of thiamethoxam:fluoxetine:fludioxonil (45:5:2.5 and (5-10):5:2.5) showed an additive effect on Fusarium oxysporum.

[0172] Indoor Example 3: Indoor Toxicity Determination of Peanut Grubs

[0173] Test target: Holotrichia oblita, 2nd instar larvae reared indoors.

[0174] Test drugs: Fluoxetine hydroxylamide, fludioxonil, thiamethoxam technical

[0175] Test basis: NY / T 1154.15-2009 "Guidelines for Indoor Bioassay Tests of Pesticides-Insecticides Part 15: Insect Dipping Method for Underground Pests".

[0176] Preparation of medicine: Dissolve the above original medicine in a suitable solvent, and then dilute it with 0.1% Tween-80 aqueous solution. Prepare single-dose mother liquor respectively, and design 5 groups of proportions according to the purpose of mixing and the activity of the medicine. Each single dose and each group of mixed proportions are prepared in 5 series of mass concentrations according to the equal ratio method.

[0177] Operation method: Immerse the target insects in the drug solution for 10 seconds and take them out, absorb the excess drug solution with filter paper, repeat each treatment 4 times, immerse 20 insects in each repetition, and set the treatment without drug as blank control.

[0178] The test insects were transferred to a glass tube filled with fresh peanut leaves as food, the tube mouth was covered with a damp black cloth, and the tube was kept and observed at 25±1℃ and relative humidity of 65%±5%.

[0179] The mortality of the test insects was investigated 72 hours after treatment. The criterion for judging the death of the test insects was obvious shrinkage of the insect body or inability to crawl normally after being pricked with a needle. The total number of insects and the number of dead insects were recorded.

[0180] Calculation method:

[0181] According to the survey data, the adjusted mortality rate of each treatment was calculated in percentage (%); the specific calculation formula is as follows:

[0182]

[0183] Where:

[0184] P——mortality rate, in percentage (%);

[0185] K——indicates the number of dead insects, in units of heads;

[0186] N——represents the total number of insects treated, in heads.

[0187]

[0188] P1——adjusted mortality rate, in percentage (%);

[0189] P t ——Treatment mortality rate, expressed as percentage (%);

[0190] P0——blank control mortality rate, in percentage (%).

[0191] If the control mortality rate is less than 5%, no correction is required; if the mortality rate is between 5% and 20%, correction should be made according to the above formula; if the control mortality rate is greater than 20%, the test needs to be repeated.

[0192] Calculation of the co-toxicity coefficient (CTC value) of the mixture:

[0193]

[0194] Where:

[0195] ATI——Actual Toxicity Index of Mixture;

[0196] S——LC of standard reagent 50 , the unit is milligrams per liter (mg / L);

[0197] M——LC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0198] TTI=TI A ×P A +TI B ×P B

[0199] Where:

[0200] TTI – Theoretical Toxicity Index of Mixtures;

[0201] TI A ——A: toxicity index of the agent;

[0202] P A ——The percentage of agent A in the mixture, in percentage (%);

[0203] TI B ——Toxicity index of agent B;

[0204] P B ——The percentage of agent B in the mixture, in percentage (%).

[0205]

[0206] Where:

[0207] CTC – Co-toxicity coefficient;

[0208] ATI——Actual Toxicity Index of Mixture;

[0209] TTI - Theoretical Toxicity Index of Mixture.

[0210] The co-toxicity coefficient of the combination CTC≥120 shows a synergistic effect; CTC≤80 shows an antagonistic effect; 80<CTC<120 shows an additive effect.

[0211] The results of the indoor biological activity test are as follows:

[0212] Table 5 The results of the combined toxicity test of the mixture of fluazifop-butyl and fludioxonil and its mixture of thiamethoxam to peanut grubs

[0213]

[0214]

[0215] Note: Fluoxetine hydroxylamide: fludioxonil = 5:2.5 (2:1) had no significant activity against peanut grubs at the designed highest concentration of 200 mg / L, and the LC cannot be calculated 50 and virulence regression equations.

[0216] The results of indoor activity test in Table 5 show that the mixture of fludioxonil and fludioxonil has no obvious activity against peanut grubs. Thiamethoxam has good toxicity to grubs, EC 50 4.6789mg.L -1 .

[0217] The test results show that the mixture of fluopicolide and fludioxonil and thiamethoxam into a ternary compound preparation can significantly increase the toxicity of thiamethoxam, enhance the preventive effect on white grubs, and significantly reduce the dosage; among them, the mass ratio of thiamethoxam:fluopicolide:fludioxonil is (15-35):5:2.5, which shows a synergistic effect on white grubs, while the mass ratio of thiamethoxam:fluopicolide:fludioxonil is 10:5:2.5 and 40:5:2.5, which shows an additive effect on white grubs.

[0218] It can be seen that the compound composition of the mixture of fluazifop-butyl and fludioxonil with thiamethoxam can significantly increase the toxicity of thiamethoxam, enhance the preventive effect on peanut grubs, and significantly reduce the dosage of the drug.

[0219] Example 4: Field efficacy test for controlling soil-borne diseases of peanuts

[0220] Test location: The field efficacy test was conducted in Shengyuan Village, Tai'an City, Shandong Province in May 2024, where serious pests and diseases occurred in previous years. The test plots belonged to peanut continuous cropping fields, and peanut soil-borne diseases occurred seriously for many years.

[0221] Test targets: peanut white rot, peanut root rot.

[0222] Experimental design: There were 11 treatments in total, including 10 treatments with chemicals and a water blank control (CK). Each treatment was repeated 4 times and the area of ​​each plot was 20 m 2 The peanut planting method in each experimental plot was the same, with artificial ridges, ridge width of 55 cm, ridge height of 20 cm, single ridge with double rows, hole spacing of 20 cm, and 2 peanuts per hole.

[0223] Application time: Mix seeds on May 5 and sow on May 6.

[0224] Survey on seedling emergence: Start the survey after the peanut seedlings have basically emerged. Randomly select 5 points in each plot, survey 20 holes at each point, record the emergence time, calculate the emergence rate, and observe the growth of peanuts.

[0225]

[0226] Disease investigation method: Peanut root rot was investigated 50 days after peanut seedlings, and white rot was investigated during the peanut harvest period. During the investigation, 100 plants were randomly sampled at 5 points in each plot, and the disease was graded and the number of diseased plants in each grade was recorded.

[0227] Peanut root rot classification standards:

[0228] Level 0, no disease;

[0229] Level 1: The roots are slightly discolored, and the discolored roots account for less than 10% of the total roots, and the plants are not wilting;

[0230] Level 3: The roots are obviously brown, and the discolored roots account for 10.1% to 30% of the total roots. The plants begin to wilt.

[0231] Level 5: Discolored roots account for 30.1% to 50% of the total root system, and the plant wilts significantly;

[0232] Level 7: Discolored roots account for 50.1% to 80% of the total root system, and the plant wilts;

[0233] Level 9: The whole plant dies.

[0234] Peanut white rot classification standards:

[0235] Level 0: no disease;

[0236] Level 1: Lesions only occur at the roots of peanuts, with 10% of cases becoming ill;

[0237] Level 3: Obvious lesions and discolored hyphae appear at the base of the peanut stems, with 30% of the cases becoming ill;

[0238] Level 5: The base of the peanut stem turns black, white mycelium is obvious, and 50% of the peanuts are infected;

[0239] Level 7: The roots of the peanut plant are covered with white hyphae, the whole plant begins to wilt, and 80% of the peanut plant is infected;

[0240] Level 9: The whole peanut plant is completely covered with white mycelium, and the roots turn completely black and die.

[0241]

[0242] Yield survey: At harvest time, the yield is measured, the yield of each plot is recorded, and the yield per hectare is converted.

[0243]

[0244] The test results are as follows:

[0245] Table 6 Effects of various treatments on peanut field emergence

[0246]

[0247]

[0248] Safety: According to the investigation of peanut germination period, the germination time of peanuts treated with various pesticides and the control with clear water was 12 days, with no significant difference. The germination rate of peanuts was above 91.50%, and there was no significant difference between the germination rate of peanuts treated with various pesticides and the control. It can be seen that the coated seeds of various treatments had no effect on the germination of peanuts in the field, and the safety was good (see Table 6).

[0249] Table 7 Control effect of each treatment on peanut white rot

[0250]

[0251] The results of the investigation on the incidence of peanut white rot during the harvest period showed that the treatment with the seed treatment suspension concentrate of fluazifop-codon-amid + fludioxonil + thiamethoxam at a dosage of 100g / 100kg of seeds had a relatively excellent control effect on peanut white rot. Compared with the control example, the control single agent and the blank control treatment, the incidence index was reduced, and it had a better control effect (see Table 7).

[0252] Table 8 Control effect of each treatment on peanut root rot

[0253]

[0254]

[0255] The results of the investigation on the incidence of peanut root rot showed that the treatment with Preparation Example 1: 32.5% fluopicolide hydroxylamine·fluthiocarb·thiamethoxam seed treatment suspension (5%+2.5%+25%) with an active ingredient dosage of 100g / 100kg of seeds had the best effect on the prevention and control of peanut root rot, followed by the treatment with Preparation Example 3: 37.5% fluopicolide hydroxylamine·fluthiocarb·thiamethoxam seed treatment suspension (5%+2.5%+20%) with an active ingredient dosage of 100g / 100kg of seeds (see Table 8).

[0256] Table 9 Effects of each treatment on peanut yield

[0257]

[0258] After the peanuts of each treatment were harvested, the yield was measured (see Table 9). Compared with the blank control, each agent treatment had a certain degree of yield increase effect. The yield increase rate of the five treatment groups of fluopyraclostrobin + fludioxonil + thiamethoxam compound preparation was 5.66% to 6.36%.

[0259] Example 5: Field test on efficacy of controlling peanut grubs

[0260] The test site is a peanut field in Fangjia Village, Jimo City, Qingdao City, Shandong Province. The soil texture of the test area is sandy loam, and the previous crop is corn. During the test, the soil moisture was good, and 35 kg of compound fertilizer was applied per mu during land preparation. The cultivation conditions and management measures of each test plot were consistent. Peanuts were sown on May 3, 2024, and ridges were covered with film. The ridge height was 10 cm, the ridge width was 80 cm, and double rows were sown, with two seeds sown in each hole. The cultivation and management conditions of each plot were consistent, and no other pests and diseases were controlled during the test.

[0261] Peanut used for testing: Huayu 22.

[0262] Test subject: Peanut grubs.

[0263] Experimental design: There are 9 treatments in total, with spraying of equal amount of water as blank control (CK). 4 replicates, randomized block arrangement, plot area 30m 2 .

[0264] Chemical treatment: Mix the test chemical with seeds one day before sowing and let them dry.

[0265] Experimental investigation: When peanuts are harvested, the number of live white grubs is investigated at fixed points, the number of damaged peanut pods is recorded, and the peanut pods are graded according to the damage.

[0266] Level 0: Pods are intact and not damaged;

[0267] Level 1: There are signs of damage on the pod skin;

[0268] Level 2: The pods have small holes, but the kernels are intact and the yield is not affected;

[0269] Level 3: There are large holes in the pods and 1 / 2 of the kernels are damaged, affecting the yield;

[0270] Level 4: Pods and kernels are damaged > 1 / 2.

[0271] Survey method: Diagonal sampling method was used. Four points were surveyed in each plot, and 0.5m 2 During the investigation, the soil was dug to a depth of 30 cm. The number of live grubs, total number of pods, and number of damaged pods at each investigation point were recorded, and the insect control effect and fruit preservation effect were calculated.

[0272] Calculation method of drug efficacy:

[0273]

[0274] Table 10 Results of the efficacy test of each treatment against peanut grubs

[0275]

[0276]

[0277] The test agent did not cause any phytotoxicity to peanuts within the dosage range of this test. The field test results show that during the entire test investigation period, the test agents showed good control effects and fruit preservation effects on peanut grubs. Among them, the average control effect and fruit preservation effect of 32.5% fluopicolide hydroxylamide, fludioxonil, and thiamethoxam seed treatment suspension (5% + 2.5% + 25%) with an active ingredient dosage of 100g / 100kg seeds for peanut grub control were the highest. The field test results show that the compound agent fluopicolide hydroxylamide + fludioxonil + thiamethoxam seed treatment suspension has a good control effect on peanut grubs. The agent is safe for peanuts within the dosage range of this test and can be promoted for use.

[0278] In summary, the present invention has verified through experiments that the mixed ratio of fluazifop-butyl, fludioxonil and thiamethoxam has a better control effect on the soil-borne diseases of peanuts such as white rot and root rot, and the underground pest peanut grub, is safe for peanuts and significantly increases yield, can effectively prevent and control the damage of pathogens and pests throughout the peanut growth period, reduces costs, reduces the amount of pesticides used, and has a good guiding significance for field promotion and use.

[0279] Although the present invention has been described in detail above with general descriptions and specific implementation schemes, it is obvious to those skilled in the art that some modifications or improvements can be made on the basis of the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention are within the scope of protection claimed by the present invention.

Claims

1. A pesticide composition containing fludioxonil and fludioxonil, characterized in that: The pesticide composition comprises a fungicide and an insecticide, wherein the fungicide is fludioxonil and fludioxonil, and the insecticide is thiamethoxam.

2. The pesticide composition according to claim 1, characterized in that The mass ratio of fluazifop-butyl to fludioxonil in the fungicide is 1:15 to 20:

1.

3. The pesticide composition according to claim 2, characterized in that The mass ratio of fludioxonil to fludioxonil in the fungicide is 1:10 to 18:1, preferably 1:5 to 9:

1.

4. The pesticide composition according to claim 1, characterized in that The mass ratio of the bactericide to the insecticide is 3:2 to 3:

25.

5. The pesticide composition according to claim 4, characterized in that The mass ratio of the bactericide to the insecticide is 3:4 to 3:16, preferably 3:4 to 3:

14.

6. The pesticide composition according to claim 1, characterized in that Based on the total weight of the pesticide composition being 100 wt%, the total content of the fungicide in the pesticide composition is 0.5% to 50%, preferably 1% to 10%, and more preferably 2% to 8%.

7. The pesticide composition according to claim 1, characterized in that Based on the total weight of the pesticide composition being 100 wt%, the content of the insecticide in the pesticide composition is 5% to 50%, preferably 10% to 40%, and more preferably 15% to 30%.

8. The pesticide composition according to claim 1, characterized in that The pesticide composition also includes an adjuvant, which is selected from one or more of a wetting agent, a dispersant, an emulsifier, a thickener, a disintegrant, an antifreeze agent, a defoaming agent, a solvent, a preservative, a stabilizer, a warning color, a film former, a synergist and a carrier.

9. The pesticide composition according to claim 1, characterized in that The pesticide composition is in the form of any one of a seed treatment suspension, a microcapsule suspension, and a dry seed dressing; Preferably, the pesticide composition is in the form of a seed treatment suspension.

10. Use of the pesticide composition according to any one of claims 1 to 9 in preventing and controlling plant diseases and / or insect pests, characterized in that: The plants include corn, wheat, soybean, rice, peanut and / or cotton; Preferably, the plant is peanut, and the peanut disease is any one of peanut black spot, peanut brown spot, peanut web spot, peanut anthracnose, peanut scorch spot, peanut purple feather disease, flower rust, peanut bacterial wilt, peanut stem rot, peanut white rot, peanut root rot, peanut yellow mosaic virus, peanut stripe virus, peanut dwarf virus, and peanut crown rot; The peanut pest is any one of white grubs, peanut aphids, cotton bollworms, beet armyworms, armyworms, and new black spider scales.

Images