An oil-soluble lip care composition containing multiple recombinant collagen proteins for repairing the lip barrier and its preparation method.

Abstract

This invention relates to the field of cosmetic technology, specifically to an oil-soluble lip care composition containing multiple recombinant collagen proteins for repairing the lip barrier and its preparation method. The raw materials for preparing the composition include 0.01-0.5 parts of recombinant collagen, 0.05-1 part of fermented plant extract, 20-40 parts of oil, and 10-20 parts of polyol. The composition of this invention contains various active ingredients, effectively repairing the lip skin barrier, exhibiting good anti-aging, moisturizing, and resistance to external factors effects, while also being low in irritation, highly safe, and providing excellent moisturizing and smoothing effects for the lips.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, specifically to an oil-soluble lip care composition containing multiple recombinant collagen proteins for repairing the skin barrier and its preparation method. Background Technology

[0002] The skin on the lips is only one-third the thickness of the skin on other parts of the body; this causes moisture to evaporate from the lips much faster than from other parts of the skin, and makes them more susceptible to external environmental stimuli (such as germs, dust, ultraviolet rays, or chemicals). Improper lip care can lead to dead skin buildup, dryness, cracking, fine lines at the corners of the lips, and bacterial infections; therefore, it is necessary to use appropriate lip care products regularly.

[0003] Currently, there are many types of lip care compositions available, with varying quality, and most are water-based products. These cannot effectively protect the lips from external environmental influences or provide effective lip care. Therefore, there is a need for an oil-soluble composition that effectively cares for the lips. Summary of the Invention

[0004] In view of the above-mentioned technical problems, the present invention proposes a technical solution, which includes an oil-soluble lip care composition containing multiple recombinant collagens for repairing the lip barrier and a method for preparing the same.

[0005] This invention provides an oil-soluble lip care composition containing multiple recombinant collagen proteins to repair the lip barrier. By weight, it comprises the following raw materials: 0.01-0.5 parts recombinant collagen, 0.05-1 parts fermented plant extract, 20-40 parts oil, and 10-20 parts polyol.

[0006] Preferably, the ingredients, by weight, include the following: 0.02-0.3 parts recombinant collagen, 0.06-0.8 parts fermented plant extract, 22-38 parts oil and fat, and 12-18 parts polyol.

[0007] Preferably, the recombinant collagen includes recombinant collagen type I (recombinant type I humanized collagen), type III (recombinant type III humanized collagen), and type XVII (recombinant type XVII humanized collagen).

[0008] Recombinant type I humanized collagen has a structure similar to natural collagen, featuring a triple helix structure that can automatically fold together to form a stable collagen triple helix. This structure allows recombinant collagen to effectively replenish and repair collagen in the skin, thereby achieving anti-wrinkle and moisturizing effects.

[0009] Recombinant type III humanized collagen has a well-defined triple helix structure, and its amino acid sequence is consistent with specific functional regions of human collagen, exhibiting excellent compatibility and preventing rejection. Recombinant type III humanized collagen possesses bidirectional regulatory, repair, and regeneration functions, replenishing the skin's missing type III collagen while simultaneously repairing damaged and aging fibroblasts, and continuing to secrete type I collagen, resulting in firm, elastic, plump, and hydrated skin. Its unique active triple helix network structure contains numerous water molecules, and the interaction between adjacent triple helices forms dense and well-organized collagen fibers. A large number of hydrophilic amino acids tightly adsorb a large number of water molecules, generating bound water, ensuring the active metabolic function of cells and keeping the skin in a hydrated state.

[0010] Recombinant XVII humanized collagen is a collagen produced through genetic engineering. It possesses an amino acid sequence completely identical to that of human collagen, exhibiting excellent biocompatibility and no immune rejection. This collagen demonstrates significant effects in skin anti-aging, repair, and regeneration, and due to its unique structure and function, it has broad application prospects in the medical and cosmetic fields. It exhibits excellent cell adhesion and migration capabilities, helping to promote skin cell regeneration and repair; its superior elasticity and pressure resistance provide better support and protection for the skin; its moisturizing effect provides ample hydration to the skin, preventing dryness and aging; furthermore, recombinant XVII humanized collagen has high biocompatibility and low immunogenicity, resulting in high safety.

[0011] Preferably, the mass ratio of recombinant collagen type I, type III and type XVII is 1:(0.5-1.2):(0.5-1.2).

[0012] Preferably, the oils include synthetic esters and vegetable oils.

[0013] Preferably, the synthetic ester includes at least one of caprylic / capric triglyceride, cetyl lactate, myristyl lactate, glyceryl trimyristate, castor oil fatty acid methyl ester, and glyceryl oleate.

[0014] Preferably, the vegetable oil includes at least one of avocado oil, camellia oil, jojoba oil, soybean oil, palm oil, almond oil, and coconut oil.

[0015] Preferably, the polyol includes at least one of ethylene glycol, propylene glycol, glycerin, polyethylene glycol, butylene glycol, and sorbitol.

[0016] Preferably, the raw materials for preparing the fermented plant extract include at least one of Gentiana macrophylla, Dipsacus asper, Codonopsis pilosula leaves, and Crab shell.

[0017] Another aspect of the present invention provides a method for preparing the composition, comprising the following steps:

[0018] Step 1: Heat the oil to melt it, add the polyol, stir and mix to obtain mixture 1;

[0019] Step 2: Cool mixture 1, add fermented plant extract, stir and mix to obtain mixture 2;

[0020] Step 3: Cool mixture 2, add recombinant collagen, stir and mix to obtain the oil-soluble lip care composition containing multiple recombinant collagen to repair the lip barrier.

[0021] Preferably, in step 1, the melting temperature is 80-95℃, and the stirring time is 0.2-1 hour.

[0022] Preferably, in step 2, the temperature is lowered to 40-55℃. The stirring time is 0.2-1 hour.

[0023] Preferably, in step 3, the temperature is lowered to 25-35°C. The stirring time is 0.2-1 hour.

[0024] Preferably, in step 3, after mixing, sterilization and packaging steps are also included.

[0025] The aforementioned method for preparing fermented plant extracts includes:

[0026] S1: Grind the raw material to less than 80 mesh to obtain powder, add 15-40 times the weight of the powder in water, boil, then stir at 60-80℃ for 2-5 hours, cool, and obtain product 1;

[0027] S2: Add serrata peptide and snail protease to product 1, enzymatically hydrolyze at 40-60℃ for 2-5 hours, then inactivate the enzymes to obtain product 2; wherein, the amount of serrata peptide and snail protease added is 300-500U / g product 1 and 200-450U / g product 1, respectively.

[0028] S3: Product 2 is sterilized, then glucose, potassium dihydrogen phosphate, and fermentation bacteria are added. It is then fermented anaerobically at 30-40℃ for 95-155 hours. After fermentation, it is sterilized to obtain product 3. Glucose and potassium dihydrogen phosphate account for 0.5-3.75% and 0.005-0.08% of the mass of product 2, respectively, and the amount of fermentation bacteria added is 2.7 × 10⁻⁶. 8 -8.2×10 8 CFU / g product 2, the fermentation bacteria consisted of Bacillus polymyxa CICC23712 and Lactobacillus reuteri CICC6126 in a ratio of 1:0.2-0.8;

[0029] S4: Product 3 is concentrated under reduced pressure to 0.5-1.5 times the mass of the powder in S1, and then anhydrous ethanol is added to adjust the ethanol mass concentration to 85-95%. The mixture is heated and stirred at 40-60℃ for 2-4 hours to obtain product 4.

[0030] S5: Product 4 is filtered to obtain filtrate, which is then subjected to ultrafiltration to obtain ultrafiltrate. The ultrafiltrate is concentrated under reduced pressure and dried to obtain the fermented plant extract.

[0031] Preferably, the raw materials include Gentiana macrophylla, Dipsacus asper (Sichuan Dipsacus asper), and Codonopsis pilosula leaves.

[0032] Preferably, the raw materials may include 0.1-2 parts of Gentiana macrophylla, 0.2-2 parts of Dipsacus asper, and 0.1-2 parts of Panax ginseng leaves, by weight.

[0033] The beneficial effects of this invention are:

[0034] This invention utilizes recombinant collagen, fermented plant extracts, oils, and polyols to prepare an oil-soluble composition. Types I, III, and XVII of the recombinant collagen impart excellent moisturizing, anti-aging, and skin-nourishing effects to the composition. The fermented plant extracts provide excellent free radical scavenging, anti-aging, and moisturizing effects. The oil components effectively isolate external environmental factors from irritating the sensitive skin of the lips, providing good resistance to external influences. The composition of this invention contains various active ingredients that effectively repair the lip skin barrier, exhibiting good anti-aging, moisturizing, and resistance to external factors, resulting in a superior user experience.

[0035] The extract prepared by this invention has a good effect in inhibiting free radicals, giving the skin anti-aging and anti-oxidation functions. When diluted to 1.5%, the extract showed scavenging rates of 77.91% and 65.48% against DPPH and ABTS free radicals, respectively.

[0036] This invention uses serrata peptides and snail protease for co-fermentation, which increases the variety and richness of active ingredients in the extract, giving the extract a better free radical scavenging effect. Moreover, the two have a mutually reinforcing relationship in enhancing the free radical scavenging effect, and have a better effect than other proteases for enzymatic hydrolysis.

[0037] The extract prepared by this invention using specific fermentation bacteria, *Bacillus polymyxa* CICC23712 and *Lactobacillus reuteri* CICC6126, exhibits better free radical scavenging effects compared to extracts obtained from other fermentation bacteria, thus imparting excellent anti-aging properties to cosmetic compositions. The two bacteria have a synergistic effect in enhancing free radical scavenging, and the extract is even more effective when the ratio is 1:0.2-0.8.

[0038] The extract prepared by this invention has a good skin moisturizing effect and can effectively give lips a plump and hydrated state. The specific enzyme combination of this invention, compared to other enzymes, makes the extract more effective. The combined enzymatic hydrolysis process of this invention gives the extract a superior moisturizing effect. The co-fermentation process using specific fermentation bacteria of this invention significantly improves the plant-based moisturizing effect of the composition.

[0039] The lip care composition prepared by this invention has low irritation and high safety.

[0040] The lip care composition prepared by this invention has a good moisturizing and hydrating effect on the lips, and a delicate texture. The composition of this invention has an excellent user experience and good performance.

[0041] The preparation method of this invention is simple, the product has excellent effects, and it has good application prospects. Detailed Implementation

[0042] The present invention will be described below with reference to specific embodiments, thereby making the various effects of the invention clearer. Those skilled in the art should understand that these specific embodiments are for illustrative purposes only and not for limiting the invention.

[0043] Polymyxin Bacillus CICC23712, Lactobacillus reuteri CICC6126, and Saccharomyces cerevisiae CICC1301 were purchased from the China Industrial Microbial Culture Collection Center.

[0044] I. Preparation and Testing of Extracts

[0045] Extract 1:

[0046] S1: Preparation of materials: 1 part Gentiana macrophylla (whole plant), 0.9 parts Dipsacus asper (whole plant) and 0.8 parts Panax quinquefolius leaves by weight; wherein the weight ratio of Gentiana macrophylla to Panax quinquefolius leaves is 1:0.8;

[0047] The raw material was pulverized in a pulverizer to a finer size than 80 mesh to obtain powder. 27 times the weight of the powder was added to water, and the mixture was boiled. Then it was cooled to 70°C and kept warm while stirring at 60 rpm for 3 hours. After cooling to room temperature, product 1 was obtained.

[0048] S2: Add serratiptase and snail protease to product 1, stir at 50℃ and 70rpm for 3.3 hours for enzymatic hydrolysis, and then inactivate the enzyme to obtain product 2; wherein, the amount of serratiptase and snail protease added is 400U / g product 1 and 300U / g product 1, respectively.

[0049] S3: Product 2 was sterilized, then glucose, potassium dihydrogen phosphate, and fermentation bacteria were added. It was fermented anaerobically at 35°C for 142 hours. After fermentation, it was sterilized to obtain product 3. Glucose and potassium dihydrogen phosphate accounted for 2.87% and 0.045% of the mass of product 2, respectively, and the amount of fermentation bacteria added was 6.60 × 10⁻⁶. 8 Product 2 (cfu / g) was fermented by a mixture of Bacillus polymyxa CICC 23712 and Lactobacillus reuteri CICC 6126 in a ratio of 1:0.5.

[0050] S4: Product 3 was concentrated under reduced pressure to 1.2 times the mass of the powder in S1, and then anhydrous ethanol was added to adjust the ethanol mass concentration to 90.5%. The mixture was stirred at 52°C and 65 rpm for 2.5 hours to obtain product 4.

[0051] S5: Product 4 is filtered through a 0.5-micron filter membrane to obtain a filtrate. The filtrate is then subjected to ultrafiltration through a 2000 Da molecular weight cutoff ultrafiltration membrane to obtain an ultrafiltrate. The ultrafiltrate is concentrated under reduced pressure at 50°C to 0.8 times the mass of the powder in S1, and then freeze-dried at -40°C to a water content of 1.75% to obtain the fermented plant extract.

[0052] Extract 2:

[0053] S1: Preparation of materials: 1.3 parts by weight of Gentiana macrophylla (whole plant), 1 part of Dipsacus asper (whole plant) and 0.7 parts of Panax notoginseng leaves; wherein the weight ratio of Gentiana macrophylla to Panax notoginseng leaves is 1:0.54.

[0054] The raw materials were pulverized in a pulverizer to a fineness below 80 mesh to obtain powder. 26.5 times the weight of the powder was added to water, and the mixture was boiled. The mixture was then cooled to 72°C and kept warm while stirring at 60 rpm for 3.2 hours. After cooling to room temperature, product 1 was obtained.

[0055] S2: Add serrata peptide and snail protease to product 1, stir at 50℃ and 70rpm for 3.5 hours for enzymatic hydrolysis, and then inactivate the enzyme to obtain product 2; wherein, the amount of serrata peptide and snail protease added is 300U / g product 1 and 400U / g product 1, respectively.

[0056] S3: Product 2 was sterilized, then glucose, potassium dihydrogen phosphate, and fermentation bacteria were added. It was fermented anaerobically at 36.5℃ for 137 hours. After fermentation, it was sterilized to obtain product 3. Glucose and potassium dihydrogen phosphate accounted for 3.03% and 0.049% of the mass of product 2, respectively, and the amount of fermentation bacteria added was 6.45 × 10⁻⁶. 8 Product 2 (cfu / g) was fermented by a mixture of Bacillus polymyxa CICC 23712 and Lactobacillus reuteri CICC 6126 in a ratio of 1:0.8.

[0057] S4: Product 3 was concentrated under reduced pressure to 1.2 times the mass of the powder in S1, and then anhydrous ethanol was added to adjust the ethanol mass concentration to 87.5%. The mixture was stirred at 52°C and 65 rpm for 3 hours to obtain product 4.

[0058] S5: Product 4 is filtered through a 0.5-micron filter membrane to obtain a filtrate. The filtrate is then subjected to ultrafiltration through a 2000 Da molecular weight cutoff ultrafiltration membrane to obtain an ultrafiltrate. The ultrafiltrate is concentrated under reduced pressure at 50°C to 0.8 times the mass of the powder in S1, and then freeze-dried at -40°C to a water content of 1.71% to obtain the fermented plant extract.

[0059] Extract 3:

[0060] S2 is as follows: Bromelain is added to product 1, and the mixture is stirred at 50℃ and 70rpm for 3.3 hours for enzymatic hydrolysis, and then the enzyme is inactivated to obtain product 2; wherein, the amount of bromelain added is 700U / g of product 1; all other steps are the same as those of extract 1.

[0061] Extract 4:

[0062] S2 is as follows: Serratia peptide enzyme is added to product 1, and the mixture is stirred at 50°C and 70 rpm for 3.3 hours for enzymatic hydrolysis, and then the enzyme is inactivated to obtain product 2; wherein, the amount of Serratia peptide enzyme added is 700 U / g product 1; all other steps are the same as those of extract 1.

[0063] Extract 5:

[0064] S2 is as follows: snail protease is added to product 1, and the mixture is stirred at 50°C and 70 rpm for 3.3 hours for enzymatic hydrolysis, and then the enzyme is inactivated to obtain product 2; wherein, the amount of snail protease added is 700 U / g of product 1; all other steps are the same as those of extract 1.

[0065] Extract 6:

[0066] S3 is as follows: Product 2 is sterilized, then glucose, potassium dihydrogen phosphate, and fermentation bacteria are added, and it is fermented anaerobically at 35°C for 142 hours. After fermentation, it is sterilized to obtain product 3; wherein, glucose and potassium dihydrogen phosphate account for 2.87% and 0.045% of the mass of product 2, respectively, and the amount of fermentation bacteria added is 6.60 × 10⁻⁶. 8 Product 2 (cfu / g) was fermented with Bacillus polymyxa CICC23712; all other aspects were the same as those of extract 1.

[0067] Extract 7:

[0068] S3 is as follows: Product 2 is sterilized, then glucose, potassium dihydrogen phosphate, and fermentation bacteria are added, and it is fermented anaerobically at 35°C for 142 hours. After fermentation, it is sterilized to obtain product 3; wherein, glucose and potassium dihydrogen phosphate account for 2.87% and 0.045% of the mass of product 2, respectively, and the amount of fermentation bacteria added is 6.60 × 10⁻⁶. 8 Product 2 (cfu / g) was fermented with Lactobacillus reuteri CI CC6126; all other aspects were the same as those of extract 1.

[0069] Extract 8:

[0070] S3 is as follows: Product 2 is sterilized, then glucose, potassium dihydrogen phosphate, and fermentation bacteria are added, and it is fermented anaerobically at 35°C for 142 hours. After fermentation, it is sterilized to obtain product 3; wherein, glucose and potassium dihydrogen phosphate account for 2.87% and 0.045% of the mass of product 2, respectively, and the amount of fermentation bacteria added is 6.60 × 10⁻⁶. 8 Product 2 (cfu / g) was fermented by a mixture of Bacillus polymyxa CICC 23712 and Lactobacillus reuteri CICC 6126 in a ratio of 1:0.1; all other components were the same as those in extract 1.

[0071] Extract 9:

[0072] S3 is as follows: Product 2 is sterilized, then glucose, potassium dihydrogen phosphate, and fermentation bacteria are added, and it is fermented anaerobically at 35°C for 142 hours. After fermentation, it is sterilized to obtain product 3; wherein, glucose and potassium dihydrogen phosphate account for 2.87% and 0.045% of the mass of product 2, respectively, and the amount of fermentation bacteria added is 6.60 × 10⁻⁶. 8 Product 2 (cfu / g) was fermented by a mixture of Bacillus polymyxa CICC 23712 and Lactobacillus reuteri CICC 6126 in a ratio of 1:1.5; all other components were the same as those in extract 1.

[0073] Extract 10:

[0074] S3 is as follows: Product 2 is sterilized, then glucose, potassium dihydrogen phosphate, and fermentation bacteria are added, and it is fermented anaerobically at 35°C for 142 hours. After fermentation, it is sterilized to obtain product 3; wherein, glucose and potassium dihydrogen phosphate account for 2.87% and 0.045% of the mass of product 2, respectively, and the amount of fermentation bacteria added is 6.60 × 10⁻⁶. 8 Product 2 (cfu / g) was fermented with Saccharomyces cerevisiae CICC1301; all other aspects were the same as those of extract 1.

[0075] A. Antioxidant effect test:

[0076] 1. DPPH free radical scavenging ability test:

[0077] Weigh out DPPH, dilute to volume with anhydrous ethanol, and prepare 2×10 -4 Store the 1 mol / L DPPH solution in a refrigerator away from light for later use.

[0078] Take 4.0 mL of a concentration of 2×10 -4 Mix 1 mol / L DPPH solution with 1.0 mL of 95% ethanol solution, let stand in the dark for half an hour, and measure the absorbance at 515 nm. Record the absorbance as A0.

[0079] Take 4.0 mL of 95% ethanol solution and 1.0 mL of the test sample (the above extract was prepared by diluting with 85% ethanol solution, and the extract concentration was 1.5%), mix well, let stand in the dark for half an hour, and measure the absorbance at 515 nm, which is recorded as Ax.

[0080] Take 4.0 mL of a concentration of 2×10 -4 The extract was prepared by diluting 1.0 mL of the test sample with 85% ethanol solution (to a concentration of 1.5%), mixing well, and allowing to stand in the dark for half an hour. The absorbance was measured at 515 nm and recorded as Ay. The DPPH scavenging rate was calculated as T = [1 - (Ay - Ax) / A0] × 100%.

[0081] 2. ABTS Free Radical Scavenging Capacity Test

[0082] Prepare a 2 mmol / L ABTS solution with distilled water. Mix 50 mL of this solution with 200 mL of a 70 mmol / L K₂S₂O₈ aqueous solution and let stand in the dark for 10 h to obtain the ABTS solution. Dilute the ABTS solution with phosphate buffer (pH 6.3) to an absorbance of 1.0 to obtain the diluted ABTS solution. Take 1 mL of the sample (the extract was prepared by diluting it with 85% ethanol, and the extract concentration was 1.5%), add 2 mL of the diluted ABTS solution, and mix well. Let stand for 10 min. Measure the absorbance at 734 nm of the different solutions using a spectrophotometer. Calculate the ABTS scavenging rate K = (A₀ - A) / A₀ × 100%, where A₀ is the absorbance of the diluted ABTS solution, and A is the absorbance of the mixture of 1 mL sample + 2 mL diluted ABTS solution.

[0083] The results of the antioxidant effects of the various extract dilutions are shown in Table 1.

[0084] Table 1: Antioxidant Effect Test

[0085] Test / 1.5% DPPH removal rate T / % ABTS clearance rate K / % Extract 1 77.91 65.48 Extract 2 76.53 64.41 Extract 3 70.68 59.92 Extract 4 75.21 63.40 Extract 5 73.49 62.07 Extract 6 69.87 59.26 Extract 7 66.34 56.54 Extract 8 74.93 63.19 Extract 9 73.06 61.75 Extract 10 64.12 54.86

[0086] Tests show that the extract prepared by this invention has a good effect in inhibiting free radicals, giving the skin anti-aging and anti-oxidation functions. When diluted to 1.5%, the extract achieved scavenging rates of 77.91% and 65.48% against DPPH and ABTS free radicals, respectively.

[0087] According to tests on extracts 1 and 3-5, the present invention uses serrata peptides and snail protease for co-fermentation, which increases the types and richness of active ingredients in the extract, endows the extract with a better free radical scavenging effect, and the two have a mutually reinforcing relationship in enhancing the free radical scavenging effect. At the same time, it has a better effect than other proteases for enzymatic hydrolysis.

[0088] Tests on extracts 1, 6-10 showed that the extract prepared by the specific fermentation bacteria *Bacillus polymyxa* CICC23712 and *Lactobacillus reuteri* CICC6126 exhibited better free radical scavenging effects compared to extracts obtained from other fermentation bacteria, thus imparting excellent anti-aging functions to cosmetic compositions. The two bacteria showed a synergistic effect in enhancing free radical scavenging, and the extract was even more effective when the ratio was 1:0.2-0.8.

[0089] B. Moisturizing effect test

[0090] 2. Moisturizing Experiment

[0091] Volunteers aged 30-45 with healthy skin and no ethanol allergy were recruited and randomly divided into 11 groups according to age, with 4 men and 4 women in each group. A 2.5cm × 2.5cm area on the right arm of each volunteer was used as the test area. The sample (diluted with ethanol to a mass concentration of 2.5%) was applied to the test area at a dosage of 0.5g per person. The moisture content of the test area was measured before sample application, 3 hours after application, and 10 hours after application (five points were randomly selected for each test, and the average value was taken), under an ambient temperature of 25℃ and humidity of 55%. The increase in skin moisture content in the test area after 3 hours and 10 hours of application (compared to the increase before sample application) was calculated. The results are shown in Table 5.

[0092] Table 5: Moisturizing Experiment Test

[0093]

[0094]

[0095] Tests show that the extracts prepared according to this invention have good skin moisturizing effects and can effectively give lips a plump and hydrated state. Extracts 1-2 have better moisturizing effects than extract 3, indicating that the specific enzyme combination of this invention has a better effect than other enzymes. Extract 1, compared to extracts 4-5, uses the combined enzymatic hydrolysis process of this invention, giving the extract a superior moisturizing effect on the composition. Composition 1, compared to compositions 6-10, uses the co-fermentation process of specific fermenting bacteria of this invention, resulting in a significant improvement in the plant-based moisturizing effect of the composition.

[0096] II. Preparation and Testing of the Composition

[0097] The composition and dosage (parts by mass) of the composition are shown in Table 2.

[0098] Table 2: Composition and Dosage of the Composition

[0099]

[0100]

[0101] The extract used in composition 1 is extract 1 as described above; the extract used in composition 2 is extract 2 as described above.

[0102] Compositions 4-11 are the same as composition 1, except that extracts 3-10 are used to replace extract 1 in sequence.

[0103] The preparation method of the above composition is as follows:

[0104] Step 1: Melt the oils (caprylic / capric triglycerides, triglycerides, avocado oil, camellia oil, coconut oil) at 85°C, add polyols (ethylene glycol, glycerol, 1,3-butanediol), and stir at 60 rpm for 0.3 hours to obtain mixture 1;

[0105] Step 2: Cool mixture 1 to 42°C, add fermented plant extract, stir at 65 rpm for 0.3 hours to obtain mixture 2;

[0106] Step 3: Cool mixture 2 to 30°C, add recombinant collagen, stir at 60 rpm for 0.4 hours, sterilize, and package to obtain the oil-soluble lip care composition containing multiple recombinant collagens to repair the lip barrier.

[0107] 1. Human patch experiment

[0108] The above composition was diluted to a concentration of 100 mg / mL with PBS buffer at pH 7 and used as a sample for testing. Volunteers aged 25-45 years were selected as test subjects and randomly assigned to groups of 12. 0.05 mL of sample was placed in a spot tester using a syringe, and then the spot test tape was applied to the skin of the volunteer's left forearm. Air was gently pressed out to ensure even sample distribution. Observations were made 24 hours later, and the results of the skin irritation test were evaluated according to the skin irritation test criteria described in Table 3. The test results are shown in Table 4.

[0109] Table 3: Standards for Skin Irritation Testing

[0110] grade degree of reaction Skin reaction 0 - Negative: No irritation, no erythema 1 ± Weak positive: Mild erythema 2 + Weak positive: Erythema 3 ++ Strongly positive: erythema, papules, vesicles 4 +++ Extremely positive: severe edema, large bullae

[0111] Table 4 Results of Skin Irritation Tests

[0112]

[0113]

[0114] As shown in Table 5, the composition prepared by this invention has low irritation and high safety.

[0115] 2. Smearing experiment

[0116] Ten volunteers aged 35-45 were recruited, five men and five women. All ten volunteers applied composition 1-2 to their lips and rated the results. Evaluation criteria included moisturizing effect (maximum 5 points, minimum 1 point), smoothing effect (maximum 5 points, minimum 1 point), and hydration effect (maximum 5 points, minimum 1 point); each test item could be scored as a decimal. The test results are shown in Table 6.

[0117] Table 6: Smearing Experiment Test

[0118] test Moisturizing rating Detailed rating Moisturizing rating Composition 1 4.7 4.6 4.8 Composition 2 4.6 4.7 4.7

[0119] According to the test results, the composition prepared by the present invention has a good moisturizing and hydrating effect on the lips, and has a delicate texture. The composition of the present invention has an excellent user experience and has good application prospects.

[0120] Obviously, those skilled in the art can make various modifications and variations to this invention without departing from its spirit and scope. Therefore, if these modifications and variations fall within the scope of the claims of this invention and their equivalents, this invention also intends to include these modifications and variations.

Claims

1. An oil-soluble lip care composition comprising a repair barrier of a plurality of recombinant collagens, characterized in that, According to mass parts, the following raw materials are included: 0.01-0.5 parts of recombinant collagen, 0.05-1 part of fermented plant extract, 20-40 parts of oil and 10-20 parts of polyhydric alcohol; The preparation method of the fermented plant extract comprises: S1: grinding the raw materials to less than 80 mesh to obtain a powder, adding water in an amount of 15-40 times the mass of the powder, boiling, then stirring at 60-80℃ for 2-5 hours, and cooling to obtain product 1; The raw materials include 0.1-2 parts of Gentiana macrophylla, 0.2-2 parts of Dipsacus asper and 0.1-2 parts of Panacis Quinquefolii Folium by weight; S2: adding serrapeptase and snail protease to product 1, and enzymatically hydrolyzing at 40-60℃ for 2-5 hours, then inactivating the enzymes to obtain product 2; wherein the amounts of serrapeptase and snail protease added are 300-500 U / g of product 1 and 200-450 U / g of product 1, respectively; S3: the product 2 is sterilized, then glucose, potassium dihydrogen phosphate and fermentation bacteria are added, and anaerobic fermentation is carried out at 30-40℃ for 95-155 hours; after fermentation is completed, sterilization is carried out to obtain product 3; wherein, the glucose and potassium dihydrogen phosphate are 0.5-3.75% and 0.005-0.08% of the mass of the product 2, and the added amount of the fermentation bacteria is 2.7×10 8 -8.2×10 8 cfu / g of the product 2, and the fermentation bacteria are composed of Paenibacillus polymyxa CICC 23712 and Lactobacillus reuteri CICC 6126 in a quantity ratio of 1:0.2-0.

8. S4: product 3 is concentrated under reduced pressure to 0.5-1.5 times the mass of the powder in S1, then anhydrous ethanol is added, the mass concentration of ethanol is adjusted to 85-95%, and heating and stirring at 40-60℃ for 2-4 hours to obtain product 4; S5: filtering product 4 to obtain a filtrate, subjecting the filtrate to ultrafiltration treatment to obtain an ultrafiltrate, concentrating the ultrafiltrate under reduced pressure, and drying to obtain the fermented plant extract.

2. An oil-soluble lip care composition containing a repair barrier of multiple recombinant collagens according to claim 1, characterized in that, The recombinant collagen includes recombinant collagen types I, III and XVII, and the mass ratio of the recombinant collagen types I, III and XVII is 1: (0.5-1.2): (0.5-1.2).

3. An oil-soluble lip care composition containing a repair barrier of multiple recombinant collagens according to claim 1, characterized in that, The oil includes synthetic esters and plant oils.

4. An oil-soluble lip care composition containing a repair barrier of multiple recombinant collagens according to claim 1, characterized in that, The polyhydric alcohol includes at least one of ethylene glycol, propylene glycol, glycerol, polyethylene glycol, butanediol and sorbitol.

5. An oil-soluble lip care composition containing a repair barrier of multiple recombinant collagens according to claim 3, characterized in that, The synthetic ester includes at least one of caprylic / capric triglyceride, cetyl lactate, myristyl lactate, trimyristin, methyl ricinoleate and glyceryl oleate.

6. An oil-soluble lip care composition containing a repair barrier of multiple recombinant collagens according to claim 3, characterized in that, The plant oil includes at least one of avocado oil, camellia oil, jojoba oil, soybean oil, palm oil, almond oil and coconut oil.

7. A method of preparing an oil-soluble lip care composition containing a plurality of recombinant collagen repair barriers according to claims 1-6, characterized in that, The method comprises the following steps: Step 1: heating and melting the oil, adding the polyhydric alcohol, and stirring and mixing to obtain a mixture 1; Step 2: cooling the mixture 1, adding the fermented plant extract, and stirring and mixing to obtain a mixture 2; Step 3: cooling the mixture 2, adding the recombinant collagen, and stirring and mixing to obtain the oil-soluble lip care composition containing multiple recombinant collagens for repairing the barrier.

8. The preparation method according to claim 7, characterized in that, The heating and melting temperature in step 1 is 80-95℃.

9. The preparation method according to claim 7, characterized in that, The temperature is cooled to 40-55℃ in step 2, and the temperature is cooled to 25-35℃ in step 3.

Images