Device and method for cleaning, disinfecting and removing nucleic acid from experimental apparatus

Abstract

The invention discloses a device and a method for cleaning, disinfecting and removing nucleic acid from experimental apparatuses. The device comprises a case, and a spraying system, an ultraviolet disinfection system, a drying system and a control display system which are arranged in the case, the method specifically comprises the following steps: (1) connecting pipelines; (2) placing the experimental apparatus on a bracket; (3) power-on self-test; (4) setting parameters; (5) chemical reagent disinfection and cleaning; (6) performing ultraviolet disinfection; (7) automatic drying; and (8) ending the operation. The cleaning, disinfecting and nucleic acid removing effects are guaranteed, no chemical reagent is left, and cleaning, disinfecting and nucleic acid removing of experimental instruments such as pipettors are achieved.

Description

Technical Field

[0001] The present invention relates to the field of biosafety and biomedical technology, and more specifically to a device and method for cleaning, disinfecting and removing nucleic acid from experimental equipment. Background Art

[0002] At present, biosafety and biomedical laboratories widely use laboratory instruments such as pipettes. Due to contact with pathogenic microorganisms or nucleic acid samples, high-pressure sterilization is time-consuming and labor-intensive, and extremely inconvenient. Most laboratories do not handle it in a timely manner, and there is a risk of cross-contamination of microorganisms and nucleic acids.

[0003] Therefore, how to efficiently clean, disinfect and remove nucleic acids from experimental equipment is an urgent problem that technical personnel in this field need to solve. Summary of the invention

[0004] In view of this, the purpose of the present invention is to provide a device and method for cleaning, disinfecting and removing nucleic acids from experimental instruments to solve the deficiencies in the prior art. The present invention ensures the effect of cleaning, disinfecting and removing nucleic acids, and has no chemical reagent residue, thereby achieving cleaning, disinfecting and removing nucleic acids from experimental instruments such as pipettes.

[0005] In order to achieve the above object, the present invention adopts the following technical solution:

[0006] A device for cleaning, disinfecting and removing nucleic acid from experimental equipment, comprising a chassis, and a spray system, an ultraviolet disinfection system, a drying system and a control display system placed in the chassis;

[0007] The chassis is a square cavity, which includes a component cavity, a disinfection cavity and a sealed cover connected in sequence from bottom to top, and the top of the disinfection cavity is sealed by the sealed cover; the sealed cover is connected to the outer shell of the chassis with a damping shaft, fixed at any angle of 0°-190°, with a sealing ring embedded inside, and magnetically attached to the outer shell of the chassis;

[0008] The spray system includes a bracket, a venturi nozzle, an infrared sensor, a pipeline, a two-position two-way solenoid valve, a peristaltic pump, a two-position three-way valve, a disinfection and nucleic acid removal reagent bottle, a cleaning reagent bottle, a disinfection and nucleic acid removal reagent, a cleaning reagent, a disinfection and nucleic acid removal reagent bottle level sensor, a cleaning reagent bottle level sensor and an air pump. The bracket, the venturi nozzle and the infrared sensor are placed in the disinfection chamber, the pipeline, the two-position two-way solenoid valve, the peristaltic pump, the two-position three-way valve, the disinfection and nucleic acid removal reagent bottle, the cleaning reagent bottle, the disinfection and nucleic acid removal reagent, the cleaning reagent, the disinfection and nucleic acid removal reagent bottle level sensor, the cleaning reagent bottle level sensor and the air pump are placed in the component chamber, the venturi nozzle, the two-position two-way solenoid valve, the peristaltic pump, the two-position three-way valve, the disinfection and nucleic acid removal reagent bottle, the cleaning reagent bottle and the air pump are respectively connected through pipelines, the disinfection and nucleic acid removal reagent is placed in the disinfection and nucleic acid removal reagent bottle, and the cleaning reagent is placed in the cleaning reagent bottle;

[0009] The UV disinfection system includes a UV lamp and a UV intensity sensor, both of which are placed in the disinfection chamber;

[0010] The drying system includes a PTC heating plate, a drying fan, a semiconductor refrigeration module, a heat dissipation fan, a water pump, a water storage tank, an exhaust fan, an exhaust hole, an air pump, a one-way valve, a waste liquid bottle, a waste liquid bottle level sensor and a temperature and humidity sensor. There are two semiconductor refrigeration modules. The PTC heating plate, the drying fan, one of the semiconductor refrigeration modules, the exhaust fan, the exhaust hole and the temperature and humidity sensor are placed in the disinfection chamber, and the other semiconductor refrigeration module, the heat dissipation fan, the water pump, the water storage tank, the air pump, the one-way valve, the waste liquid bottle and the waste liquid bottle level sensor are placed in the component chamber. The semiconductor refrigeration module, the water pump, the water storage tank, the exhaust hole, the air pump, the one-way valve and the waste liquid bottle are connected by pipelines respectively.

[0011] The control and display system includes a control circuit and a touch screen. The control circuit is connected to an infrared sensor, a two-position two-way solenoid valve, a peristaltic pump, a two-position three-way valve, a disinfection and nucleic acid removal reagent bottle level sensor, a cleaning reagent bottle level sensor, a waste liquid bottle level sensor, an air pump, an ultraviolet lamp, an ultraviolet intensity sensor, a PTC heater, a drying fan, a semiconductor refrigeration module, a cooling fan, a water pump, an exhaust fan, an air pump, a temperature and humidity sensor and a touch screen through lines to control its operation.

[0012] Furthermore, the bracket is provided with 1-10 experimental equipment placement positions with hook and groove structures, which are inclined at 10°-90°.

[0013] A further beneficial effect of adopting the above method is that the bracket is a processed part used to place experimental instruments, and can place 1-10 experimental instruments including pipettes at the same time; the tilted placement makes it easy for residual reagents to be discharged to the waste liquid bottle through the vacuum pump.

[0014] Furthermore, the above-mentioned Venturi nozzle is composed of a conical drill mouth with a circular cross-section, a cylindrical throat and a conical outlet, which is installed at the bottom of the bracket, and the number is consistent with the number of experimental instrument placement positions on the bracket, forming a one-to-one correspondence; the Venturi nozzle is provided with an anti-clogging injection hole, and the injection hole is aligned with the inner cavity of the experimental instrument; the Venturi nozzle is connected to the air pump and the disinfection chamber in sequence through pipes, forming a gas loop with the disinfection chamber; the Venturi nozzle is connected to the liquid loop of the two-position two-way solenoid valve, the peristaltic pump, the two-position three-way valve, the disinfection and nucleic acid removal reagent bottle and the cleaning reagent bottle in sequence through pipes, and the disinfection and nucleic acid removal reagent and the cleaning reagent are sprayed to the surface and inner cavity of the experimental instrument in micron-level factors through the injection hole, so as to achieve the purpose of cleaning, disinfection and nucleic acid removal.

[0015] Furthermore, the infrared sensors are installed below the bracket, and the number of the infrared sensors is consistent with the number of experimental equipment placement positions on the bracket, forming a one-to-one correspondence.

[0016] A further beneficial effect of the above is that the infrared sensor is used to monitor the position and quantity of the experimental equipment.

[0017] Furthermore, the above-mentioned pipe is a common part, generally a silicone tube.

[0018] Furthermore, the above-mentioned two-position two-way solenoid valve is a purchased part and is a normally closed solenoid valve. Its function is to open the valve when needed, so that the disinfection and nucleic acid removal reagents or cleaning reagents can be sprayed through the pipeline for disinfection or cleaning.

[0019] Furthermore, the peristaltic pump is an externally purchased component, and its function is to allow the disinfection and nucleic acid removal reagent or the cleaning reagent to circulate in the pipeline without contacting the liquid.

[0020] Furthermore, the above-mentioned two-position three-way valve is an outsourced part, and its function is to switch the pipeline between the disinfection and nucleic acid removal reagent and the cleaning reagent.

[0021] Furthermore, the above-mentioned disinfection and nucleic acid removal reagent bottle and cleaning reagent bottle are blow-molded parts with a volume of 100-2200mL, including a bottle body, a sealing cover, a liquid outlet pipe, and an air inlet pipe. A groove handle is provided on the bottle body, and the sealing cover is composed of a threaded cover and a sealing disc. A liquid outlet pipe and a liquid inlet pipe are distributed on the sealing disc, and the sealing cover is tightly matched with the bottle body.

[0022] Furthermore, the above-mentioned disinfection and nucleic acid removal reagent is a hydrogen peroxide solution containing a surfactant; the mass concentration of the hydrogen peroxide solution is 1.5%-6%; the surfactant is sodium dodecyl sulfate (SDS) with a mass concentration of 0.01%-0.1%, and is dissolved and diluted with a buffer solution with a pH of 7.0-8.0; the disinfection and nucleic acid removal reagent is pre-installed in a disinfection and nucleic acid removal reagent bottle with a volume of 100-2200mL, and is connected to two two-position three-way valves and a peristaltic pump in sequence through a pipeline, and then refluxed to the disinfection and nucleic acid removal reagent bottle to form a loop; on the loop, a three-way is connected to the two-position three-way valve and a Venturi nozzle in sequence by a pipeline, and spray disinfection is performed by a siphon effect.

[0023] The further beneficial effect of the above method is that the hydrogen peroxide solution acts as a DNA / RNA destroyer to remove nucleic acids and effectively remove amplified fragments, plasmids, genomic DNA and RNA. The role of sodium dodecyl sulfate is to moisten, emulsify, disperse, degrease and decontaminate the pollutants of the experimental equipment.

[0024] Furthermore, the above-mentioned cleaning reagent is sterile purified water or water for injection; the cleaning reagent is pre-installed in a cleaning reagent bottle with a volume of 100-2200mL, and is connected to two two-position three-way valves and a peristaltic pump in sequence through a pipeline, and then flows back into the cleaning reagent bottle to form a loop; on the loop, a three-way pipe is connected to the two-position three-way valve and a Venturi nozzle in sequence, and spray cleaning is performed through a siphon effect.

[0025] Furthermore, the above-mentioned liquid level sensor is a purchased part, and its function is to monitor the liquid volume of the disinfection and nucleic acid removal reagent bottle, the cleaning reagent bottle and the waste liquid bottle.

[0026] Furthermore, the above-mentioned air pump is a purchased part, and its function is to pump the disinfection and nucleic acid removal reagents and cleaning reagents through the pipeline to the Venturi nozzle, and then spray them in micron-level factors to the pipettes and other experimental instruments placed in the disinfection chamber to achieve the purpose of disinfection and nucleic acid removal.

[0027] Furthermore, the above-mentioned ultraviolet lamp is a UVC deep ultraviolet waterproof sterilization and disinfection module, which is installed at the bottom of the bracket. The number of the ultraviolet lamps is consistent with the number of experimental instrument placement positions on the bracket, forming a one-to-one correspondence.

[0028] A further beneficial effect of the above method is that the UV lamp can be used to disinfect the experimental instruments such as pipettes placed in the disinfection chamber. When cleaning and disinfecting the experimental instruments to remove nucleic acids, hydrogen peroxide solution is used in combination with ultraviolet rays to enhance the disinfection effect. For more serious contamination, multiple cycles of disinfection are performed.

[0029] Furthermore, the above-mentioned ultraviolet intensity sensor is used to monitor the ultraviolet intensity, automatically calculate the irradiation time according to the set ultraviolet irradiation dose, ensure the disinfection effect, and promptly prompt replacement when the intensity of the ultraviolet lamp is weakened.

[0030] Furthermore, the above-mentioned PTC heating plate is a commonly used component, which is used to heat and automatically control the temperature to dry experimental instruments such as pipettes placed in the disinfection chamber.

[0031] Furthermore, the drying fan is a commonly used component, and its function is to transfer the heat generated by the PTC heating plate to the disinfection chamber to dry the experimental instruments such as the pipette placed in the disinfection chamber.

[0032] Furthermore, the semiconductor refrigeration module is composed of a heat sink, a semiconductor refrigeration plate and a water-cooling plate which are bonded together in sequence, with thermal conductive glue coated between them; the cold water generated by the semiconductor refrigeration module installed in the component cavity circulates through the water-cooling plate of the semiconductor refrigeration module installed in the disinfection cavity through a pipeline.

[0033] A further beneficial effect of adopting the above method is that the semiconductor refrigeration module plays a role in heat dissipation, while ensuring that the air is isolated from the outside and the disinfection chamber is sealed.

[0034] Furthermore, the above-mentioned heat dissipation fan is a commonly used component, and its function is to dissipate the heat generated by the semiconductor refrigeration module.

[0035] Furthermore, the water pump is a commonly used component, and its function is to circulate the cold water generated by the semiconductor refrigeration module installed in the component cavity through the pipeline through the water-cooling plate of the semiconductor refrigeration module installed in the disinfection cavity, thereby playing a role in heat dissipation.

[0036] Furthermore, the above-mentioned water storage tank is a commonly used component, and its function is to store water.

[0037] Furthermore, the exhaust fan is a commonly used component, which blows out the mist inside the disinfection chamber through the exhaust hole, thereby accelerating the drying process.

[0038] Furthermore, the above-mentioned vacuum pump is a commonly used component, and its function is to discharge air mist, residual reagents, and condensed water droplets into the waste liquid bottle.

[0039] Furthermore, the one-way valve is a commonly used component, and its function is to ensure that mist and liquid can only flow from the disinfection chamber to the waste liquid bottle and cannot flow in the opposite direction.

[0040] Furthermore, the above-mentioned waste liquid bottle is a commonly used item, which is used to store air mist, residual reagents, and condensed water droplets.

[0041] Furthermore, the temperature and humidity sensor is a commonly used component, which is used to monitor the temperature and humidity inside the disinfection chamber and automatically determine the drying time according to the set end point temperature and humidity.

[0042] Furthermore, the function of the above-mentioned control circuit is to calculate the spray volume according to the spray rate and time of the Venturi nozzle, and calculate the remaining volume in combination with the pre-installed volume of the disinfection and nucleic acid removal reagent and the cleaning reagent and display it on the touch screen, prompting timely replacement of the disinfection and nucleic acid removal reagent and the cleaning reagent.

[0043] Furthermore, the above-mentioned touch screen is a commonly used component, and its function is to display and set operating parameters and status.

[0044] Furthermore, the chassis is a square cavity in which the various components are installed.

[0045] A method for cleaning, disinfecting and removing nucleic acids from laboratory equipment, using the above-mentioned device for cleaning, disinfecting and removing nucleic acids from laboratory equipment, specifically comprising the following steps:

[0046] (1) Connect the disinfection and nucleic acid removal reagent bottle and the cleaning reagent bottle to the pipeline;

[0047] (2) Place the experimental apparatus on the stand;

[0048] (3) Power-on self-test: including the functional status of each electronic component;

[0049] (4) Setting parameters: Setting the cleaning, disinfection, nucleic acid removal and drying parameters on the touch screen, including reagent volume, spray time, ultraviolet irradiation dose, endpoint temperature and endpoint humidity, and starting the operation;

[0050] (5) Chemical reagent disinfection and cleaning: Infrared sensors monitor the location and quantity of experimental equipment. Disinfection and nucleic acid removal reagents and cleaning reagents are sprayed in micrometer-level factors on the surface and inner cavity of the experimental equipment through the Venturi nozzle in sequence. The reagent volume and spray time are automatically determined according to the set cleaning, disinfection and nucleic acid removal parameters;

[0051] (6) Ultraviolet disinfection: Turn on the ultraviolet lamp and use ultraviolet disinfection in combination, and automatically determine the irradiation time according to the set ultraviolet irradiation dose and the monitored ultraviolet intensity;

[0052] (7) Automatic drying: Turn on the drying system, the drying fan transfers the heat generated by the PTC heater to the disinfection chamber, blows the hot air to the surface and inner cavity of the experimental equipment such as the pipette through the Venturi nozzle, the semiconductor refrigeration module condenses the mist into water droplets, and the exhaust fan blows the mist inside the disinfection chamber out through the exhaust hole. The air mist, residual reagents and condensed water droplets are discharged to the waste liquid bottle through the vacuum pump. The drying end time is automatically determined according to the set end temperature and humidity and by monitoring the temperature and humidity;

[0053] (8) End the operation.

[0054] It can be seen from the above technical solution that compared with the prior art, the beneficial effects of the present invention are as follows:

[0055] 1. It integrates chemical reagent disinfection and cleaning, and ultraviolet combined disinfection mode, achieving multiple purposes of cleaning, disinfecting and removing nucleic acids from experimental equipment such as pipettes;

[0056] 2. The spray hole of the Venturi nozzle is aligned with the inner cavity of the experimental equipment. The disinfection and nucleic acid removal reagents and cleaning reagents are sprayed to the surface and inner cavity of the experimental equipment through this hole in micron-level factors. The number is consistent with the number of experimental equipment, showing a one-to-one correspondence, which ensures the effect of cleaning, disinfection and nucleic acid removal;

[0057] 3. Infrared sensors, ultraviolet intensity sensors and temperature and humidity sensors monitor the location and quantity of experimental equipment, ultraviolet intensity and temperature and humidity respectively, and automatically determine the number of Venturi nozzles opened, ultraviolet lamp irradiation time and drying end time, realizing automation and intelligence;

[0058] 4. On the basis of using as little chemical reagents as possible and cleaning the residues, nucleic acids are removed by cleaning and disinfecting experimental equipment such as pipettes to prevent cross-contamination of microorganisms and nucleic acids. BRIEF DESCRIPTION OF THE DRAWINGS

[0059] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying creative work.

[0060] Figure 1 This is a schematic diagram of the structure of the device for cleaning, disinfecting and removing nucleic acid from experimental equipment in Example 1-2;

[0061] Figure 2 This is a schematic diagram of the structure of a multi-spray system for cleaning, disinfecting and removing nucleic acid from experimental equipment in Example 2;

[0062] Among them, 1- bracket, 2- Venturi nozzle, 3- infrared sensor, 4- pipeline, 5- two-position two-way solenoid valve, 6- peristaltic pump, 7- two-position three-way valve, 8- disinfection and nucleic acid removal reagent bottle, 9- cleaning reagent bottle, 10- disinfection and nucleic acid removal reagent, 11- cleaning reagent, 121- disinfection and nucleic acid removal reagent bottle level sensor, 122- cleaning reagent bottle level sensor, 123- waste liquid bottle level sensor, 13- air pump, 14- ultraviolet lamp, 1 5-ultraviolet intensity sensor, 16-PTC heating plate, 17-drying fan, 18-semiconductor refrigeration module, 19-cooling fan, 20-water pump, 21-water storage tank, 22-exhaust fan, 23-exhaust hole, 24-exhaust pump, 25-check valve, 26-waste liquid bottle, 27-temperature and humidity sensor, 28-control circuit, 29-touch screen, 30-chassis, 301-disinfection chamber, 302-component chamber, 303-sealed cover. DETAILED DESCRIPTION

[0063] Embodiments of the present invention are described in detail below, examples of which are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are intended to be used to explain the present invention, and should not be construed as limiting the present invention.

[0064] In the description of the present invention, it should be understood that the terms "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside" and "outside" etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0065] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. In the description of the present invention, the meaning of "plurality" is two or more, unless otherwise clearly and specifically defined.

[0066] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0067] In the present invention, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may include that the first and second features are in direct contact, or may include that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, a first feature being "above", "above" and "above" a second feature includes that the first feature is directly above and obliquely above the second feature, or simply indicates that the first feature is higher in level than the second feature. A first feature being "below", "below" and "below" a second feature includes that the first feature is directly below and obliquely below the second feature, or simply indicates that the first feature is lower in level than the second feature.

[0068] Example 1

[0069] Devices for cleaning, disinfecting and removing nucleic acids from experimental equipment, such as Figure 1 As shown, it includes a chassis 30, and a spray system, an ultraviolet disinfection system, a drying system and a control display system placed in the chassis 30;

[0070] The chassis 30 is a square cavity, which includes a component cavity 302, a disinfection cavity 301 and a sealed cover 303 connected in sequence from bottom to top. The top of the disinfection cavity 301 is sealed by the sealed cover 303. The sealed cover 303 is connected to the outer shell of the chassis 30 by a damping shaft, fixed at 0°, and has a sealing ring embedded inside, and is embedded with the outer shell of the chassis 30 by magnetic attraction.

[0071] The spray system includes a bracket 1, a venturi nozzle 2, an infrared sensor 3, a pipeline 4, a two-position two-way solenoid valve 5, a peristaltic pump 6, a two-position three-way valve 7, a disinfection nucleic acid removal reagent bottle 8, a cleaning reagent bottle 9, a disinfection nucleic acid removal reagent 10, a cleaning reagent 11, a disinfection nucleic acid removal reagent bottle level sensor 121, a cleaning reagent bottle level sensor 122 and an air pump 13. The bracket 1, the venturi nozzle 2 and the infrared sensor 3 are placed in the disinfection chamber 301, the pipeline 4, the two-position two-way solenoid valve 5, the peristaltic pump 6, the two-position three-way valve 7, the disinfection nucleic acid removal reagent bottle 8, the cleaning reagent bottle 9, the disinfection nucleic acid removal reagent 10, the cleaning reagent 11, the disinfection nucleic acid removal reagent bottle level sensor 121, the cleaning reagent bottle level sensor 122 and the air pump 13. The nucleic acid removal reagent bottle 8, the cleaning reagent bottle 9, the disinfection nucleic acid removal reagent 10, the cleaning reagent 11, the disinfection nucleic acid removal reagent bottle level sensor 121, the cleaning reagent bottle level sensor 122 and the air pump 13 are placed in the component cavity 302, the venturi nozzle 2, the two-position two-way solenoid valve 5, the peristaltic pump 6, the two-position three-way valve 7, the disinfection nucleic acid removal reagent bottle 8, the cleaning reagent bottle 9 and the air pump 13 are respectively connected through the pipeline 4, the disinfection nucleic acid removal reagent 10 is placed in the disinfection nucleic acid removal reagent bottle 8, and the cleaning reagent 11 is placed in the cleaning reagent bottle 9;

[0072] Among them, the bracket 1 is provided with a groove structure for placing experimental instruments, which is inclined at 30°;

[0073] The venturi nozzle 2 is composed of a conical drill mouth with a circular cross section, a cylindrical throat and a conical outlet, and is installed at the bottom of the bracket 1. The number of the nozzles is consistent with the number of experimental equipment placement positions on the bracket 1, and is in a one-to-one correspondence; the venturi nozzle 2 is provided with an anti-clogging injection hole, and the injection hole is aligned with the inner cavity of the experimental equipment; the venturi nozzle 2 is connected to the air pump 13 and the disinfection chamber 301 in sequence through the pipeline 4, respectively, to form a gas loop with the disinfection chamber 301; the venturi nozzle 2 is connected to the liquid loop of the two-position two-way solenoid valve 5, the peristaltic pump 6, the two-position three-way valve 7, the disinfection nucleic acid removal reagent bottle 8 and the cleaning reagent bottle 9 in sequence through the pipeline 4, and the disinfection nucleic acid removal reagent 10 and the cleaning reagent 11 are sprayed to the surface and inner cavity of the experimental equipment in micron-level factors through the injection hole;

[0074] The infrared sensors 3 are installed under the bracket 1, and the number of the infrared sensors 3 is consistent with the number of experimental equipment placement positions on the bracket 1, forming a one-to-one correspondence;

[0075] The disinfection and nucleic acid removal reagent bottle 8 and the cleaning reagent bottle 9 are blow-molded parts with a volume of 500 mL, including a bottle body, a sealing cover, a liquid outlet pipe, and a liquid inlet pipe. A groove handle is provided on the bottle body, and the sealing cover is composed of a threaded cover and a sealing disc. A liquid outlet pipe and a liquid inlet pipe are distributed on the sealing disc, and the sealing cover is tightly matched with the bottle body;

[0076] The disinfection and nucleic acid removal reagent 10 is a hydrogen peroxide solution containing a surfactant; the mass concentration of the hydrogen peroxide solution is 4.0%; the surfactant is sodium dodecyl sulfate with a mass concentration of 0.05%, which is dissolved and diluted with a buffer solution with a pH of 7.0; the disinfection and nucleic acid removal reagent 10 is pre-installed in a disinfection and nucleic acid removal reagent bottle 8 with a volume of 300 mL, and is connected to two two-position three-way valves 7 and a peristaltic pump 6 in sequence through a pipeline 4, and then flows back to the disinfection and nucleic acid removal reagent bottle 8 to form a loop; on the loop, the pipeline 4 is connected to the two-position three-way valve 7 and the Venturi nozzle 2 in sequence through a three-way, and spray disinfection is carried out through the siphon effect;

[0077] The cleaning reagent 11 is sterile purified water; the cleaning reagent 11 is pre-installed in a cleaning reagent bottle 9 with a volume of 300 mL, and is sequentially connected to two two-position three-way valves 7 and a peristaltic pump 6 through a pipeline 4, and then flows back into the cleaning reagent bottle 9 to form a loop; on the loop, the pipeline 4 is sequentially connected to the two-position three-way valve 7 and the Venturi nozzle 2 through a three-way, and spray cleaning is performed through a siphon effect;

[0078] The ultraviolet disinfection system includes an ultraviolet lamp 14 and an ultraviolet intensity sensor 15, both of which are placed in the disinfection chamber 301; wherein the ultraviolet lamp 14 is a UVC deep ultraviolet waterproof sterilization and disinfection module, the ultraviolet intensity is 30000μW, and is installed at the bottom of the bracket 1, and the number is consistent with the number of experimental equipment placement positions on the bracket 1, in a one-to-one correspondence; the ultraviolet intensity sensor 15 is used to monitor the ultraviolet intensity, automatically calculate the irradiation time according to the set ultraviolet irradiation dose, and promptly prompt to replace the ultraviolet lamp when the intensity of the ultraviolet lamp is weakened;

[0079] The drying system includes a PTC heating plate 16, a drying fan 17, a semiconductor refrigeration module 18, a heat dissipation fan 19, a water pump 20, a water storage tank 21, an exhaust fan 22, an exhaust hole 23, an air pump 24, a one-way valve 25, a waste liquid bottle 26, a waste liquid bottle level sensor 123 and a temperature and humidity sensor 27. There are two semiconductor refrigeration modules 18, one of the PTC heating plate 16, the drying fan 17, the semiconductor refrigeration module 18, the exhaust fan 22, the exhaust hole 23 and the temperature and humidity sensor 27 are placed in the disinfection chamber 301, and the other semiconductor refrigeration module 18, the heat dissipation fan 19, the water pump 20 , a water storage tank 21, an air pump 24, a one-way valve 25, a waste liquid bottle 26 and a waste liquid bottle level sensor 123 are placed in the component cavity 302, and the semiconductor refrigeration module 18, the water pump 20, the water storage tank 21, the exhaust hole 23, the air pump 24, the one-way valve 25 and the waste liquid bottle 26 are respectively connected through the pipeline 4; wherein the semiconductor refrigeration module 18 is composed of a heat sink, a semiconductor refrigeration sheet and a water cooling sheet which are sequentially attached together, and a thermal conductive glue is applied between them; the cold water generated by the semiconductor refrigeration module 18 installed in the component cavity 302 circulates through the pipeline 4 and flows through the water cooling sheet of the semiconductor refrigeration module 18 installed in the disinfection cavity 301;

[0080] The control and display system includes a control circuit 28 and a touch screen 29. The control circuit 28 is connected to an infrared sensor 3, a two-position two-way solenoid valve 5, a peristaltic pump 6, a two-position three-way valve 7, a disinfection and nucleic acid removal reagent bottle level sensor 121, a cleaning reagent bottle level sensor 122, a waste liquid bottle level sensor 123, an air pump 13, an ultraviolet lamp 14, an ultraviolet intensity sensor 15, a PTC heating plate 16, a drying fan 17, a semiconductor refrigeration module 18, a cooling fan 19, a water pump 20, an exhaust fan 22, an air pump 24, a temperature and humidity sensor 27 and a touch screen 29 through lines.

[0081] Example 2

[0082] Devices for cleaning, disinfecting and removing nucleic acids from experimental equipment, such as Figure 1 As shown, it includes a chassis 30, and a spray system, an ultraviolet disinfection system, a drying system and a control display system placed in the chassis 30;

[0083] The chassis 30 is a square cavity, which includes a component cavity 302, a disinfection cavity 301 and a sealed cover 303 connected in sequence from bottom to top. The top of the disinfection cavity 301 is sealed by the sealed cover 303. The sealed cover 303 is connected to the outer shell of the chassis 30 by a damping shaft, fixed at 0°, and has a sealing ring embedded inside, and is embedded with the outer shell of the chassis 30 by magnetic attraction.

[0084] like Figure 1 , Figure 2 As shown, the spray system includes a bracket 1, a venturi nozzle 2, an infrared sensor 3, a pipeline 4, a two-position two-way solenoid valve 5, a peristaltic pump 6, a two-position three-way valve 7, a disinfection nucleic acid removal reagent bottle 8, a cleaning reagent bottle 9, a disinfection nucleic acid removal reagent 10, a cleaning reagent 11, a disinfection nucleic acid removal reagent bottle level sensor 121, a cleaning reagent bottle level sensor 122 and an air pump 13. The bracket 1, the venturi nozzle 2 and the infrared sensor 3 are placed in the disinfection chamber 301, and the pipeline 4, the two-position two-way solenoid valve 5, the peristaltic pump 6, the two-position three-way valve 7, The disinfection and nucleic acid removal reagent bottle 8, the cleaning reagent bottle 9, the disinfection and nucleic acid removal reagent 10, the cleaning reagent 11, the disinfection and nucleic acid removal reagent bottle level sensor 121, the cleaning reagent bottle level sensor 122 and the air pump 13 are placed in the component cavity 302, the venturi nozzle 2, the two-position two-way solenoid valve 5, the peristaltic pump 6, the two-position three-way valve 7, the disinfection and nucleic acid removal reagent bottle 8, the cleaning reagent bottle 9 and the air pump 13 are respectively connected through the pipeline 4, the disinfection and nucleic acid removal reagent 10 is placed in the disinfection and nucleic acid removal reagent bottle 8, and the cleaning reagent 11 is placed in the cleaning reagent bottle 9;

[0085] Among them, the bracket 1 is provided with 5 groove structures for placing experimental instruments, which are inclined at 45°;

[0086] The venturi nozzle 2 is composed of a conical drill mouth with a circular cross section, a cylindrical throat and a conical outlet, and is installed at the bottom of the bracket 1. The number of the venturi nozzles 2 is consistent with the number of experimental equipment placement positions on the bracket 1, which is 5, in a one-to-one correspondence; the venturi nozzle 2 is provided with an anti-clogging injection hole, and the injection hole is aligned with the inner cavity of the experimental equipment; the venturi nozzle 2 is connected to 5 two-position two-way solenoid valves 5 in sequence through the pipeline 4, and then connected in parallel to the circulation loop of the disinfection and nucleic acid removal reagent 10 and the cleaning reagent 11, and the disinfection and nucleic acid removal reagent 10 and the cleaning reagent 11 are sprayed to the surface and inner cavity of the experimental equipment through the injection hole in a micron-level factor;

[0087] The infrared sensors 3 are installed under the bracket 1, and the number of the infrared sensors 3 is the same as the number of experimental equipment placement positions on the bracket 1, which is 5, in a one-to-one correspondence;

[0088] The disinfection and nucleic acid removal reagent bottle 8 and the cleaning reagent bottle 9 are blow-molded parts with a volume of 500 mL, including a bottle body, a sealing cover, a liquid outlet pipe, and a liquid inlet pipe. A groove handle is provided on the bottle body, and the sealing cover is composed of a threaded cover and a sealing disc. A liquid outlet pipe and a liquid inlet pipe are distributed on the sealing disc, and the sealing cover is tightly matched with the bottle body;

[0089] The disinfection and nucleic acid removal reagent 10 is a hydrogen peroxide solution containing a surfactant; the mass concentration of the hydrogen peroxide solution is 4.0%; the surfactant is sodium dodecyl sulfate with a mass concentration of 0.05%, which is dissolved and diluted with a buffer solution with a pH of 7.5; the disinfection and nucleic acid removal reagent 10 is pre-installed in a disinfection and nucleic acid removal reagent bottle 8 with a volume of 300 mL, and is connected to two two-position three-way valves 7 and a peristaltic pump 6 in sequence through a pipeline 4, and then flows back to the disinfection and nucleic acid removal reagent bottle 8 to form a loop; in the loop, the pipeline 4 is connected to the two-position three-way valve 7 and the Venturi nozzle 2 in sequence through a three-way, and spray disinfection is carried out through the siphon effect;

[0090] The cleaning reagent 11 is sterile water for injection; the cleaning reagent 11 is pre-installed in a cleaning reagent bottle 9 with a volume of 300 mL, and is sequentially connected to two two-position three-way valves 7 and a peristaltic pump 6 through a pipeline 4, and then flows back into the cleaning reagent bottle 9 to form a loop; on the loop, the pipeline 4 is sequentially connected to the two-position three-way valve 7 and the Venturi nozzle 2 through a three-way, and spray cleaning is performed through a siphon effect;

[0091] The air pump 13 is connected to the venturi nozzles 2 through a pipeline, the number of which is the same as the number of the venturi nozzles 2, which is 5, in a one-to-one correspondence, and then connected in parallel to the disinfection chamber 301;

[0092] The ultraviolet disinfection system includes an ultraviolet lamp 14 and an ultraviolet intensity sensor 15, both of which are placed in the disinfection chamber 301; wherein the ultraviolet lamp 14 is a UVC deep ultraviolet waterproof sterilization and disinfection module, the ultraviolet intensity is 30000μW, and is installed at the bottom of the bracket 1, and the number is consistent with the number of experimental equipment placement positions on the bracket 1, which is 5, in a one-to-one correspondence; the ultraviolet intensity sensor 15 is used to monitor the ultraviolet intensity, automatically calculate the irradiation time according to the set ultraviolet irradiation dose, and promptly prompt to replace the ultraviolet lamp when the intensity of the ultraviolet lamp is weakened;

[0093] The drying system includes a PTC heating plate 16, a drying fan 17, a semiconductor refrigeration module 18, a heat dissipation fan 19, a water pump 20, a water storage tank 21, an exhaust fan 22, an exhaust hole 23, an air pump 24, a one-way valve 25, a waste liquid bottle 26, a waste liquid bottle level sensor 123 and a temperature and humidity sensor 27. There are two semiconductor refrigeration modules 18, one of the PTC heating plate 16, the drying fan 17, the semiconductor refrigeration module 18, the exhaust fan 22, the exhaust hole 23 and the temperature and humidity sensor 27 are placed in the disinfection chamber 301, and the other semiconductor refrigeration module 18, the heat dissipation fan 19, the water pump 20 , a water storage tank 21, an air pump 24, a one-way valve 25, a waste liquid bottle 26 and a waste liquid bottle level sensor 123 are placed in the component cavity 302, and the semiconductor refrigeration module 18, the water pump 20, the water storage tank 21, the exhaust hole 23, the air pump 24, the one-way valve 25 and the waste liquid bottle 26 are respectively connected through the pipeline 4; wherein the semiconductor refrigeration module 18 is composed of a heat sink, a semiconductor refrigeration sheet and a water cooling sheet which are sequentially attached together, and a thermal conductive glue is applied between them; the cold water generated by the semiconductor refrigeration module 18 installed in the component cavity 302 circulates through the pipeline 4 and flows through the water cooling sheet of the semiconductor refrigeration module 18 installed in the disinfection cavity 301;

[0094] The control and display system includes a control circuit 28 and a touch screen 29. The control circuit 28 is connected to an infrared sensor 3, a two-position two-way solenoid valve 5, a peristaltic pump 6, a two-position three-way valve 7, a disinfection and nucleic acid removal reagent bottle level sensor 121, a cleaning reagent bottle level sensor 122, a waste liquid bottle level sensor 123, an air pump 13, an ultraviolet lamp 14, an ultraviolet intensity sensor 15, a PTC heating plate 16, a drying fan 17, a semiconductor refrigeration module 18, a cooling fan 19, a water pump 20, an exhaust fan 22, an air pump 24, a temperature and humidity sensor 27 and a touch screen 29 through lines.

[0095] Example 3

[0096] The method for cleaning, disinfecting and removing nucleic acid from experimental equipment adopts the device for cleaning, disinfecting and removing nucleic acid from experimental equipment in Example 1, and specifically comprises the following steps:

[0097] (1) Connect the disinfection and nucleic acid removal reagent bottle 8 and the cleaning reagent bottle 9 to the pipeline 4;

[0098] (2) Place a pipette on bracket 1;

[0099] (3) Power-on self-test: including the functional status of each electronic component;

[0100] (4) Setting parameters: Set the cleaning, disinfection, nucleic acid removal and drying parameters on the touch screen 29, including reagent volume 1 mL, spray time 10 s, and ultraviolet irradiation dose 2200 μW·min / cm 2 , end point temperature 30℃ and end point humidity 50%, start operation;

[0101] (5) Chemical reagent disinfection and cleaning: The infrared sensor 3 monitors the position and quantity of the experimental equipment, which is a pipette. The disinfection and nucleic acid removal reagent 10 and the cleaning reagent 11 are sprayed in micrometers to the surface and inner cavity of the pipette through the Venturi nozzle 2 in sequence. The reagent volume 1 mL and the spray time 10 s are automatically determined according to the set cleaning, disinfection and nucleic acid removal parameters;

[0102] (6) Ultraviolet disinfection: Turn on the ultraviolet lamp 14 and use ultraviolet disinfection in combination. According to the set ultraviolet irradiation dose of 2200 μW·min / cm 2 And the monitored UV intensity of 30000μW automatically determines the irradiation time of 30min;

[0103] (7) Automatic drying: Turn on the drying system, the drying fan 17 transfers the heat generated by the PTC heating plate 16 to the disinfection chamber 301, blows the hot air to the surface and inner cavity of the pipette through the Venturi nozzle 2, the semiconductor refrigeration module 18 condenses the mist into water droplets, the exhaust fan 22 blows the mist inside the disinfection chamber 301 out through the exhaust hole 23, and the air mist, residual reagent and condensed water droplets are discharged to the waste liquid bottle 26 through the vacuum pump 24. The drying end time is automatically determined according to the set end temperature and humidity and by monitoring the temperature and humidity;

[0104] (8) End the operation.

[0105] Example 4

[0106] The method for cleaning, disinfecting and removing nucleic acid from experimental equipment adopts the device for cleaning, disinfecting and removing nucleic acid from experimental equipment in Example 2, and specifically comprises the following steps:

[0107] (1) Connect the disinfection and nucleic acid removal reagent bottle 8 and the cleaning reagent bottle 9 to the pipeline 4;

[0108] (2) Place 5 pipettes on rack 1;

[0109] (3) Power-on self-test: including the functional status of each electronic component;

[0110] (4) Setting parameters: Set the cleaning, disinfection, nucleic acid removal and drying parameters on the touch screen 29, including reagent volume 1 mL, spray time 10 s, and ultraviolet irradiation dose 2200 μW·min / cm 2 , end point temperature 30℃ and end point humidity 50%, start operation;

[0111] (5) Chemical reagent disinfection and cleaning: The infrared sensor 3 monitors the position and number of the experimental equipment, which is 5 pipettes. The disinfection and nucleic acid removal reagent 10 and the cleaning reagent 11 are sprayed in micrometers to the surface and inner cavity of the experimental equipment through the Venturi nozzle 2 in sequence. The reagent volume 1mL and the spray time 10s are automatically determined according to the set cleaning, disinfection and nucleic acid removal parameters;

[0112] (6) Ultraviolet disinfection: Turn on the ultraviolet lamp 14 and use ultraviolet disinfection in combination. According to the set ultraviolet irradiation dose of 2200 μW·min / cm 2 And the monitored UV intensity of 30000μW automatically determines the irradiation time of 30min;

[0113] (7) Automatic drying: Turn on the drying system, the drying fan 17 transfers the heat generated by the PTC heating plate 16 to the disinfection chamber 301, blows the hot air to the surface and inner cavity of the pipette through the Venturi nozzle 2, the semiconductor refrigeration module 18 condenses the mist into water droplets, the exhaust fan 22 blows the mist inside the disinfection chamber 301 out through the exhaust hole 23, and the air mist, residual reagent and condensed water droplets are discharged to the waste liquid bottle 26 through the vacuum pump 24. The drying end time is automatically determined according to the set end temperature and humidity and by monitoring the temperature and humidity;

[0114] (8) End the operation.

[0115] Performance Testing

[0116] 1. Cleaning effect

[0117] (1) Test steps: 5 pipettes were placed on a support 1, and the pipettes were cleaned and disinfected to remove nucleic acids according to the method of Example 4. Then, the pipettes were rinsed with 10 mL of distilled water to collect the solution. The residual hydrogen peroxide was determined by the xylenol orange method, and the residual sodium dodecyl sulfate was determined by spectrophotometry.

[0118] (2) Test results: as shown in Table 1.

[0119] Table 1 Cleaning test results (n=5)

[0120] Reagent name Hydrogen Peroxide Sodium Lauryl Sulfate Residue after cleaning / μg / L 0 0

[0121] As shown in Table 1, no hydrogen peroxide and sodium dodecyl sulfate remained on the pipette after cleaning.

[0122] 2. Disinfection effect

[0123] (1) Test steps: Place 5 pipettes on the stand 1 and pipette 1 mL of the test bacterial suspension (10 5 -10 6 cfu / mL) was sprayed onto the surface and inner cavity of the pipette, and the pipette was cleaned, disinfected and nucleic acid was removed according to the method of Example 4. Then, the pipette was rinsed with 100 mL of sterile saline to collect the solution, and the microbial count was performed according to the membrane filtration method.

[0124] (2) Test results: as shown in Table 2.

[0125] Table 2 Disinfection test results (n=5)

[0126] Test strains Escherichia coli Staphylococcus aureus Candida albicans Microbial count before disinfection / cfu <![CDATA[5.6×10 6 ]]> <![CDATA[2.1×10 6 ]]> <![CDATA[5.3×10 5 ]]> Microbial count after disinfection / cfu 0 0 0

[0127] As can be seen from Table 2, no microorganisms were detected on the cleaned pipettes.

[0128] 3. Nucleic acid removal effect

[0129] (1) Experimental steps: 5 pipettes were placed on a support 1, and the Escherichia coli genomic DNA solution was sprayed onto the surface and inner cavity of the pipettes respectively. The pipettes were cleaned and disinfected to remove nucleic acid according to the method of Example 4, and then the pipettes were rinsed with 10 mL of distilled water to collect the solution, and the DNA residue was detected according to the PCR method;

[0130] (2) Test results: as shown in Table 3.

[0131] Table 3 Nucleic acid removal test results (n = 5)

[0132] PCR Escherichia coli genomic DNA Positive Control + Experimental Group -

[0133] As shown in Table 3, no Escherichia coli genomic DNA remained on the pipette after cleaning.

[0134] The above experiments show that the present invention ensures the effect of cleaning, disinfecting and removing nucleic acids without any chemical reagent residue, thereby achieving the cleaning, disinfecting and removing nucleic acids of experimental instruments such as pipettes.

[0135] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine different embodiments or examples described in this specification.

[0136] Although the embodiments of the present invention have been shown and described above, it is to be understood that the above embodiments are exemplary and are not to be construed as limitations of the present invention. A person skilled in the art may change, modify, replace and vary the above embodiments within the scope of the present invention.

Claims

1. A device for cleaning, disinfecting and removing nucleic acid from experimental equipment, characterized in that: It comprises a chassis (30), and a spray system, an ultraviolet disinfection system, a drying system and a control display system placed in the chassis (30); The chassis (30) is a square cavity, comprising a component cavity (302), a disinfection cavity (301) and a sealing cover (303) connected in sequence from bottom to top, and the top of the disinfection cavity (301) is sealed by the sealing cover (303); the sealing cover (303) is connected to the outer shell of the chassis (30) by a damping shaft, fixed at any angle of 0°-190°, and has a sealing ring embedded inside, and is embedded with the outer shell of the chassis (30) by magnetic attraction; The spray system comprises a support (1), a venturi nozzle (2), an infrared sensor (3), a pipeline (4), a two-position two-way electromagnetic valve (5), a peristaltic pump (6), a two-position three-way valve (7), a disinfection nucleic acid removal reagent bottle (8), a cleaning reagent bottle (9), a disinfection nucleic acid removal reagent (10), a cleaning reagent (11), a disinfection nucleic acid removal reagent bottle level sensor (121), a cleaning reagent bottle level sensor (122) and an air pump (13). The support (1), the venturi nozzle (2) and the infrared sensor (3) are placed in the disinfection chamber (301); the pipeline (4), the two-position two-way electromagnetic valve (5), the peristaltic pump (6), the two-position three-way valve (7), the disinfection nucleic acid removal reagent bottle ( 8), the cleaning reagent bottle (9), the disinfection and nucleic acid removal reagent (10), the cleaning reagent (11), the disinfection and nucleic acid removal reagent bottle level sensor (121), the cleaning reagent bottle level sensor (122) and the air pump (13) are placed in the component cavity (302), the Venturi nozzle (2), the two-position two-way solenoid valve (5), the peristaltic pump (6), the two-position three-way valve (7), the disinfection and nucleic acid removal reagent bottle (8), the cleaning reagent bottle (9) and the air pump (13) are respectively connected through the pipeline (4), the disinfection and nucleic acid removal reagent (10) is placed in the disinfection and nucleic acid removal reagent bottle (8), and the cleaning reagent (11) is placed in the cleaning reagent bottle (9); The ultraviolet disinfection system comprises an ultraviolet lamp (14) and an ultraviolet intensity sensor (15), both of which are placed in the disinfection chamber (301); The drying system comprises a PTC heating plate (16), a drying fan (17), a semiconductor refrigeration module (18), a heat dissipation fan (19), a water pump (20), a water storage tank (21), an exhaust fan (22), an exhaust hole (23), an exhaust pump (24), a one-way valve (25), a waste liquid bottle (26), a waste liquid bottle level sensor (123) and a temperature and humidity sensor (27). The semiconductor refrigeration module (18) has two PTC heating plates (16), the drying fan (17), one of the semiconductor refrigeration modules (18), the exhaust fan (22), the exhaust hole (23) and the temperature and humidity sensor (27). The level sensor (27) is placed in the disinfection chamber (301), another one of the semiconductor refrigeration modules (18), the cooling fan (19), the water pump (20), the water storage tank (21), the air pump (24), the one-way valve (25), the waste liquid bottle (26) and the waste liquid bottle level sensor (123) are placed in the component chamber (302), and the semiconductor refrigeration module (18), the water pump (20), the water storage tank (21), the exhaust hole (23), the air pump (24), the one-way valve (25) and the waste liquid bottle (26) are respectively connected through the pipeline (4); The control and display system comprises a control circuit (28) and a touch screen (29), wherein the control circuit (28) is respectively connected to the infrared sensor (3), the two-position two-way solenoid valve (5), the peristaltic pump (6), the two-position three-way valve (7), the disinfection and nucleic acid removal reagent bottle level sensor (121), the cleaning reagent bottle level sensor (122), the waste liquid bottle level sensor (123), the air pump (13), the ultraviolet lamp (14), the ultraviolet intensity sensor (15), the PTC heating plate (16), the drying fan (17), the semiconductor refrigeration module (18), the cooling fan (19), the water pump (20), the exhaust fan (22), the air pump (24), the temperature and humidity sensor (27) and the touch screen (29) through lines.

2. A device for cleaning, disinfecting and removing nucleic acid from experimental equipment according to claim 1, characterized in that: The support (1) is provided with 1 to 10 experimental equipment placement positions with hook and groove structures, which are inclined at 10° to 90°.

3. A device for cleaning, disinfecting and removing nucleic acid from experimental equipment according to claim 1, characterized in that: The Venturi nozzle (2) is composed of a conical drill hole with a circular cross section, a cylindrical throat and a conical outlet, and is installed at the bottom of the bracket (1). The number of the Venturi nozzles is consistent with the number of experimental equipment placement positions on the bracket (1), and is in a one-to-one correspondence; the Venturi nozzle (2) is provided with an anti-clogging injection hole, and the injection hole is aligned with the inner cavity of the experimental equipment; the Venturi nozzle (2) is connected to the air pump (13) and the disinfection chamber (301) in sequence through the pipeline (4), respectively, to form a gas circuit with the disinfection chamber (301); the Venturi nozzle (2) is connected to the liquid circuit of the two-position two-way solenoid valve (5), the peristaltic pump (6), the two-position three-way valve (7), the disinfection nucleic acid removal reagent bottle (8) and the cleaning reagent bottle (9) in sequence through the pipeline (4), and the disinfection nucleic acid removal reagent (10) and the cleaning reagent (11) are sprayed to the surface and inner cavity of the experimental equipment in micron-level factors through the injection hole.

4. A device for cleaning, disinfecting and removing nucleic acid from experimental equipment according to claim 1, characterized in that: The infrared sensors (3) are installed below the bracket (1), and the number of the infrared sensors (3) is consistent with the number of experimental equipment placement positions on the bracket (1), forming a one-to-one correspondence.

5. A device for cleaning, disinfecting and removing nucleic acid from experimental equipment according to claim 1, characterized in that: The disinfection nucleic acid removal reagent bottle (8) and the cleaning reagent bottle (9) are blow-molded parts with a volume of 100-2200 mL, including a bottle body, a sealing cover, a liquid outlet pipe, and a liquid inlet pipe. A groove handle is provided on the bottle body, and the sealing cover is composed of a threaded cover and a sealing disc. The liquid outlet pipe and the liquid inlet pipe are distributed on the sealing disc, and the sealing cover is tightly matched with the bottle body.

6. A device for cleaning, disinfecting and removing nucleic acid from experimental equipment according to claim 1, characterized in that: The disinfection and nucleic acid removal reagent (10) is a hydrogen peroxide solution containing a surfactant; the mass concentration of the hydrogen peroxide solution is 1.5%-6%; the surfactant is sodium dodecyl sulfate, the mass concentration is 0.01%-0.1%, and is dissolved and diluted with a buffer solution with a pH of 7.0-8.0; the disinfection and nucleic acid removal reagent (10) is pre-installed in the disinfection and nucleic acid removal reagent bottle (8), with a volume of 100-2200mL, and is connected to the two two-position three-way valves (7) and the peristaltic pump (6) in sequence through the pipeline (4), and then flows back into the disinfection and nucleic acid removal reagent bottle (8) to form a loop; on the loop, the pipeline (4) is connected to the two-position three-way valve (7) and the Venturi nozzle (2) in sequence through a three-way, and spray disinfection is performed through a siphon effect.

7. A device for cleaning, disinfecting and removing nucleic acid from experimental equipment according to claim 1, characterized in that: The cleaning reagent (11) is sterile purified water or water for injection; the cleaning reagent (11) is pre-installed in the cleaning reagent bottle (9) with a volume of 100-2200 mL, and is sequentially connected to the two two-position three-way valves (7) and the peristaltic pump (6) through the pipeline (4), and then flows back into the cleaning reagent bottle (9) to form a loop; on the loop, the pipeline (4) is sequentially connected to the two-position three-way valve (7) and the Venturi nozzle (2) through a three-way connection, and spray cleaning is performed through a siphon effect.

8. A device for cleaning, disinfecting and removing nucleic acid from experimental equipment according to claim 1, characterized in that: The ultraviolet lamp (14) is a UVC deep ultraviolet waterproof sterilization and disinfection module, which is installed at the bottom of the bracket (1), and the number is consistent with the number of experimental equipment placement positions on the bracket (1), forming a one-to-one correspondence; the ultraviolet intensity sensor (15) is used to monitor the ultraviolet intensity, automatically calculate the irradiation time according to the set ultraviolet irradiation dose, and promptly prompt replacement when the ultraviolet lamp intensity decreases.

9. A device for cleaning, disinfecting and removing nucleic acid from experimental equipment according to claim 1, characterized in that: The semiconductor refrigeration module (18) is composed of a heat sink, a semiconductor refrigeration plate and a water cooling plate which are bonded together in sequence, with heat conductive glue applied between them; the cold water generated by the semiconductor refrigeration module (18) installed in the component cavity (302) circulates through the water cooling plate of the semiconductor refrigeration module (18) installed in the disinfection cavity (301) through the pipeline (4).

10. A method for cleaning, disinfecting and removing nucleic acid from experimental equipment, characterized in that: The device for cleaning, disinfecting and removing nucleic acid from experimental equipment as claimed in claim 1 specifically comprises the following steps: (1) Connecting the disinfection and nucleic acid removal reagent bottle (8) and the cleaning reagent bottle (9) to the pipeline (4); (2) Place the experimental apparatus on the bracket (1); (3) Power-on self-test: including the functional status of each electronic component; (4) Setting parameters: Setting cleaning, disinfection, nucleic acid removal parameters and drying parameters on the touch screen (29), including reagent volume, spray time, ultraviolet irradiation dose, endpoint temperature and endpoint humidity, and starting the operation; (5) Chemical reagent disinfection and cleaning: The infrared sensor (3) monitors the position and quantity of the experimental equipment, and the disinfection and nucleic acid removal reagent (10) and the cleaning reagent (11) are sprayed in micrometer-level factors to the surface and inner cavity of the experimental equipment through the Venturi nozzle (2) in sequence, and the reagent volume and spray time are automatically determined according to the set cleaning, disinfection and nucleic acid removal parameters; (6) Ultraviolet disinfection: turning on the ultraviolet lamp (14), combining ultraviolet disinfection, and automatically determining the irradiation time according to the set ultraviolet irradiation dose and the monitored ultraviolet intensity; (7) Automatic drying: Turn on the drying system, the drying fan (17) transfers the heat generated by the PTC heating plate (16) to the disinfection chamber (301), blows the hot air to the surface and inner cavity of the pipette through the Venturi nozzle (2), the semiconductor refrigeration module (18) condenses the mist into water droplets, the exhaust fan (22) blows the mist inside the disinfection chamber (301) out through the exhaust hole (23), the air mist, residual reagent and condensed water droplets are discharged to the waste liquid bottle (26) through the vacuum pump (24), and the drying end time is automatically determined according to the set end temperature and humidity and by monitoring the temperature and humidity; (8) End the operation.

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