A gene editing method for Kentucky bluegrass
By constructing the CRISPR/Cas9 gene editing system for grass early maturing grass and editing the PpTCP4 gene at a fixed point, the problem of imperfect genetic transformation system of grass early maturing grass is solved, efficient gene editing is achieved, the tillering of grass early maturing grass is promoted, and the genetic breeding ability of grass early maturing grass is improved.
Patent Information
- Application Number
- CN202510087983.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-21
- Publication Date
- 2025-04-18
- Estimated Expiration
- 2045-01-21
AI Technical Summary
The existing genetic transformation system of grass early maturing grass is not yet perfect, limiting the development and utilization of this species.
The CRISPR/Cas9 gene editing system for grass early maturing grass was constructed to edit the PpTCP4 gene in a targeted manner, and promote the tillering of grass early maturing grass by targeted knockout or reducing the expression of PpTCP4 gene.
Gene editing plants were successfully obtained, and an efficient and complete genetic transformation system for grass early maturing grass was established, with a gene editing efficiency of 0.67%, providing new research basis and technical reference for the directional improvement of grass early maturing grass and the creation of high-quality germplasm resources.
Smart Images
Figure CN119506347B_ABST
Abstract
Citation Information
Patent Citations
Resistance gene SpCIPK25, protein, expression vector and application
CN114875049A