Integrated seminal fluid sampling and seminal fluid reproduction analysis equipment

Abstract

The invention discloses integrated seminal fluid sampling and seminal fluid reproduction analysis equipment, which belongs to the technical field of biological detection and analysis, and comprises a box body, the box body is provided with a detection assembly; the box body is provided with a pick-and-place assembly; a cover glass piece-by-piece feeding assembly is arranged below the taking and placing assembly of the waste discharging station, and the cover glass piece-by-piece feeding assembly is fixedly connected with the box body; a piece-by-piece feeding assembly used for feeding piece by piece is arranged below the taking and placing assembly at the glass slide feeding station and the cover glass feeding station, the piece-by-piece feeding assembly is fixedly connected with a partition plate of the box body, and the piece-by-piece feeding assembly at the glass slide feeding station is used for feeding glass slides piece by piece; a piece-by-piece feeding assembly at the cover glass feeding station is used for feeding cover glass piece by piece; a pipetting assembly for sampling seminal fluid is connected into the box body. In this way, continuous and automatic detection analysis is achieved.

Description

Technical Field

[0001] The present invention relates to the technical field of biological detection and analysis, and in particular to an integrated semen sampling and semen reproductive analysis device. Background Art

[0002] In human reproductive clinics and animal husbandry production, sperm quality detection and analysis is of great significance for the application of artificial insemination technology and breeding screening technology. Generally, analytical equipment is used to analyze and process it.

[0003] For example, Chinese patent CN217820028U discloses a portable integrated sperm analyzer, the front of which is provided with a touch screen, a button operation and status display panel, and a stage access door; the inside of the shell is provided with a stepper driver, a main control circuit board, a PC host, a power module, and an optical imaging mechanism; the optical imaging mechanism frame is provided with a stage assembly, a phase contrast converter assembly, a light source, an objective lens converter assembly, and a camera. This sperm analyzer integrates optical imaging mechanisms, PC hosts, touch screens, and other equipment to form a portable multifunctional all-in-one machine, which can be easily transported to the semen sampling site of the breeding farm for on-site instant detection and analysis. Moreover, this sperm analyzer has a high degree of automation and intelligence, and does not require cumbersome manual adjustments to the optical imaging mechanism, which can save detection time and improve the accuracy of detection indicators. In addition, this sperm analyzer can also upload the test results to the cloud in a timely manner for data sharing.

[0004] Although the above structure can detect and analyze, it requires manual cooperation for slide loading, semen sampling and cover glass loading, and cannot realize automated analysis.

[0005] Based on this, the present invention designs an integrated semen sampling and semen reproductive analysis device to solve the above problems. Summary of the invention

[0006] In view of the above-mentioned shortcomings of the prior art, the present invention provides an integrated semen sampling and semen reproductive analysis device.

[0007] To achieve the above objectives, the present invention is implemented through the following technical solutions:

[0008] Integrated semen sampling and semen reproductive analysis equipment, including box:

[0009] An equiangular rotation assembly for equiangular rotation is connected to the left side of the top of the partition of the box body;

[0010] The box body is equipped with a detection component for detection at the detection station of the equi-angle rotating component;

[0011] The box is equipped with pick-and-place components for handling materials at the waste material unloading station, slide loading station and cover glass loading station of the equiangular rotating components;

[0012] A cover glass sheet loading assembly is provided below the pick-and-place assembly of the waste material unloading station, and the cover glass sheet loading assembly is fixedly connected to the box body;

[0013] A piece-by-piece loading assembly for feeding the glass slides one by one is provided below the pick-and-place assembly at the glass slide loading station and the cover glass loading station. The piece-by-piece loading assembly is fixedly connected to the partition of the box body. The piece-by-piece loading assembly at the glass slide loading station is used for feeding the glass slides one by one, and the piece-by-piece loading assembly at the cover glass loading station is used for feeding the cover glass one by one.

[0014] A pipetting assembly for semen sampling is connected to the box, and the left end of the pipetting assembly is located directly above the semen loading station of the equiangular rotating assembly;

[0015] Three groups of square limit frames are provided on the right side of the top of the partition of the box body; and the square limit frames are respectively connected with the semen cup storage rack, the gun tip storage rack and the waste gun tip collection assembly, the semen cups containing semen are inserted in the rectangular array on the semen cup storage rack, the gun tip storage rack is connected with the gun tips in the rectangular array, and the gun tip storage rack and the waste gun tip collection assembly are located on the rear side of the semen cup storage rack, and the waste gun tip collection assembly is located on the right side of the gun tip storage rack.

[0016] Furthermore, the equal-angle rotating assembly includes a rotating disk, a slide slot, a through hole and an equal-angle driving assembly. The equal-angle driving assembly is fixedly mounted on the top of the partition of the box body. The driving end of the equal-angle driving assembly is connected to the rotating disk. The outer edge of the rotating disk has slide slots at equal intervals along the circumference. The rotating disk has a through hole at the bottom of the slide slot, and the bottom of the through hole passes through the bottom of the rotating disk.

[0017] Furthermore, the detection component includes an electron microscope, a second support frame and a light source. The second support frame is fixedly installed on the top of the partition of the box body. The side walls of the second support frame are fixedly connected with the electron microscope and the light source in sequence from top to bottom, and the electron microscope is located below the slide slot and above the slide slot.

[0018] Furthermore, the pick-and-place assembly includes a first cylinder, a first support frame, a transverse movement module and a suction cup; the first support frame is fixedly installed on the top of the partition of the box body, the upper end of the side wall of the first support frame is fixedly connected to the transverse movement module, the driving end of the transverse movement module is fixedly connected to the first cylinder, and the driving end of the first cylinder is fixedly connected to the suction cup.

[0019] Furthermore, the piece-by-piece loading assembly includes a second cylinder, a material storage barrel, a second groove, a push plate, a third support frame and a support seat. The third support frame is fixedly installed on the top of the partition of the box body. The top of the third support frame is fixedly connected to the second cylinder and the support seat. The top of the support seat is provided with a second groove. The end of the top of the support seat close to the second cylinder is fixedly connected to the material storage barrel. The driving end of the second cylinder is fixedly connected to the push plate. The outer wall of the push plate is fitted and slidably connected to the inner wall of the second groove. The top of the push plate is fitted and slidably connected to the bottom of the material storage barrel. The end of the support seat away from the second cylinder is located below the corresponding pick-and-place assembly.

[0020] Furthermore, the storage cylinder at the slide loading station stores slides, and the storage cylinder at the cover glass loading station stores cover glasses.

[0021] Furthermore, the pipetting assembly includes an XYZ-axis drive assembly, a fourth mounting bracket, an electromagnetic lock and a manual pipette. The XYZ-axis drive assembly is fixedly mounted on the top of the box body, and the side wall of the Z-axis drive end of the XYZ-axis drive assembly is fixedly connected to the fourth mounting bracket. The fourth mounting bracket is fixedly connected to the electromagnetic lock and the manual pipette in sequence from top to bottom. The electromagnetic lock is located above the manual pipette, and the gun tip is plugged into the suction end of the manual pipette.

[0022] Furthermore, the waste gun tip collection assembly includes a collection box, a first groove and a cross plate. The collection box is inserted into a square limit frame. The collection box is fixedly connected to a cross plate near the top of the semen cup storage rack. The cross plate is provided with a first groove at the end away from the semen cup storage rack.

[0023] Furthermore, the size of the first groove is smaller than the size of the top of the gun tip, and the size of the first groove is larger than the size of the part where the manual pipette is connected to the gun tip.

[0024] Beneficial effect: the present invention requires that the empty waste gun tip collection component, the gun tip storage rack filled with gun tips, and the semen cup storage rack filled with semen be installed in a square limit frame, the equal-angle rotation component rotates, the pick-and-place component of the slide loading station takes out the slides in the piece-by-piece loading component of the slide loading station and puts them on the equal-angle rotation component, the equal-angle rotation component drives the slide to rotate to the semen loading station, the pipetting component assembles the gun tip from the gun tip storage rack, the gun tip moves to the semen cup of the semen cup storage rack, the gun tip absorbs a certain amount of semen, the pipetting component drives the gun tip to deliver the absorbed semen to the slide of the semen loading station, then the pipetting component moves the gun tip to the waste gun tip collection component, the waste gun tip collection component Cooperate with the pipetting component to collect the gun tip, the equal-angle rotation component drives the semen slide to rotate to the cover glass loading station, the pick-and-place component at the cover glass loading station takes out the cover glass in the piece-by-piece loading component at the cover glass loading station and places it on the slide on the equal-angle rotation component, the equal-angle rotation component drives the slide to continue to rotate to the detection component, the detection component performs detection and analysis, after the detection component detects and analyzes, the equal-angle rotation component continues to drive the detection waste to rotate to the waste unloading station, the pick-and-place component of the waste unloading station takes out the detected waste and places it into the cover glass piece-by-piece loading component, the cover glass piece-by-piece loading component is discharged after the waste is discharged, and the above actions are repeated to realize continuous and automated detection and analysis. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the prior art descriptions are briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention, and for ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0026] Figure 1 The integrated semen sampling and semen reproductive analysis device of the present invention is a three-dimensional Figure 1 ;

[0027] Figure 2 It is a front view of the integrated semen sampling and semen reproductive analysis device of the present invention;

[0028] Figure 3 It is a left view of the integrated semen sampling and semen reproductive analysis device of the present invention;

[0029] Figure 4 For along Figure 3 AA direction section Figure 1 ;

[0030] Figure 5 For along Figure 3 AA direction section Figure 2 ;

[0031] Figure 6 For along Figure 3 BB direction cross section Figure 1 ;

[0032] Figure 7 For along Figure 3 BB direction cross section Figure 2 ;

[0033] Figure 8 for Figure 6 A magnified view of the structure at C;

[0034] Fig. 9 It is a schematic diagram of the structure of the piece-by-piece loading assembly;

[0035] Fig.10 For along Figure 3 DD direction cross-sectional view.

[0036] The numbers in the figure represent:

[0037] 1. Box; 2. Waste gun head collection assembly; 21. Collection box; 22. First groove; 23. Horizontal plate; 3. Pick-up and place assembly; 31. First cylinder; 32. First support frame; 33. Transverse movement module; 34. Suction cup; 4. Detection assembly; 41. Electron microscope; 42. Second support frame; 43. Light source; 5. Sheet-by-sheet loading assembly; 51. Second cylinder; 52. Storage barrel; 53. Second groove; 54. Push plate; 55 , the third support frame; 56, the support seat; 6, the cover glass piece by piece loading assembly; 7, the equal-angle rotation assembly; 71, the rotating disk; 72, the slide slot; 73, the through hole; 74, the equal-angle drive assembly; 8, the pipetting assembly; 81, the XYZ axis drive assembly; 82, the fourth mounting frame; 83, the electromagnetic lock; 84, the manual pipette; 85, the gun tip; 9, the semen cup storage rack; 10, the gun tip storage rack; 11, the square limit frame. DETAILED DESCRIPTION

[0038] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0039] The present invention will be further described below in conjunction with the embodiments.

[0040] For example, see Figure 1-Figure 10 , integrated semen sampling and semen reproductive analysis equipment, including box 1:

[0041] An equiangular rotation assembly 7 for equiangular rotation is connected to the left side of the top of the partition of the box body 1;

[0042] The box body 1 is equipped with a detection component 4 for detection at the detection station of the equal-angle rotation component 7;

[0043] The box body 1 is equipped with a pick-and-place assembly 3 for transporting materials at the waste material unloading station, the slide loading station and the cover glass loading station of the equiangular rotating assembly 7;

[0044] A cover glass sheet loading assembly 6 is provided below the pick-and-place assembly 3 of the waste material unloading station, and the cover glass sheet loading assembly 6 is fixedly connected to the box body 1;

[0045] A piece-by-piece loading assembly 5 for feeding piece-by-piece is provided below the pick-and-place assembly 3 at the slide loading station and the cover glass loading station. The piece-by-piece loading assembly 5 is fixedly connected to the partition of the box body 1. The piece-by-piece loading assembly 5 at the slide loading station is used for feeding the slides piece-by-piece, and the piece-by-piece loading assembly 5 at the cover glass loading station is used for feeding the cover glass piece-by-piece;

[0046] A pipetting assembly 8 for semen sampling is connected to the box 1, and the left end of the pipetting assembly 8 is located directly above the semen loading station of the equiangular rotating assembly 7;

[0047] Three groups of square limit frames 11 are provided on the right side of the top of the partition of the box body 1; and the square limit frames 11 are respectively connected with the semen cup storage rack 9, the gun tip storage rack 10 and the waste gun tip collection assembly 2, the semen cups containing semen are inserted in the rectangular array on the semen cup storage rack 9, the gun tip storage rack 10 is connected with the gun tips 85 in the rectangular array, and the gun tip storage rack 10 and the waste gun tip collection assembly 2 are located on the rear side of the semen cup storage rack 9, and the waste gun tip collection assembly 2 is located on the right side of the gun tip storage rack 10.

[0048] The empty waste gun tip collection component 2, the gun tip storage rack 10 filled with gun tips 85, and the semen cup storage rack 9 filled with semen are required to be installed in the square limit frame 11, the equal-angle rotation component 7 rotates, the pick-and-place component 3 of the slide loading station takes out the slides in the slide loading component 5 of the slide loading station and puts them on the equal-angle rotation component 7, the equal-angle rotation component 7 drives the slide to rotate to the semen loading station, the pipetting component 8 assembles the gun tip 85 from the gun tip storage rack 10, the gun tip 85 moves to the semen cup of the semen cup storage rack 9, the gun tip 85 absorbs a certain amount of semen, the pipetting component 8 drives the gun tip 85 to deliver the absorbed semen to the slide of the semen loading station, and then the pipetting component 8 moves the gun tip 85 to the waste gun tip collection component 2, the waste gun tip collection The collecting component 2 and the pipetting component 8 cooperate to collect the gun tip 85, the equi-angle rotating component 7 drives the slide glass with the semen to rotate to the cover glass loading station, the pick-and-place component 3 at the cover glass loading station takes out the cover glass in the piece-by-piece loading component 5 at the cover glass loading station and places it on the slide glass on the equi-angle rotating component 7, the equi-angle rotating component 7 drives the slide glass to continue to rotate to the detection component 4, the detection component 4 performs detection and analysis, after the detection component 4 detects and analyzes, the equi-angle rotating component 7 continues to drive the detection waste to rotate to the waste unloading station, the pick-and-place component 3 of the waste unloading station takes out the detected waste and places it into the cover glass piece-by-piece loading component 6, the cover glass piece-by-piece loading component 6 discharges the waste, and repeats the above actions to achieve continuous and automated detection and analysis.

[0049] The equi-angle rotating component 7 includes a rotating disk 71, a glass slide slot 72, a through hole 73 and an equi-angle driving component 74. The equi-angle driving component 74 is fixedly installed on the top of the partition of the box body 1. The driving end of the equi-angle driving component 74 is connected to the rotating disk 71. The outer edge of the rotating disk 71 is provided with glass slide slots 72 at equal intervals along the circumferential direction. The rotating disk 71 is provided with a through hole 73 at the bottom of the glass slide slot 72, and the bottom of the through hole 73 passes through the bottom of the rotating disk 71.

[0050] The equal angle driving assembly 74 includes a cam divider and a motor, an input end of the cam divider and an output end of the motor, and the output end of the cam divider is fixedly connected to the rotating disk 71;

[0051] The equi-angle driving assembly 74 of the equi-angle rotating assembly 7 drives the rotating disk 71 to rotate at an equi-angle, the rotating disk 71 drives the slide slot 72 to rotate, and the slide slot 72 drives the slide to rotate to different working positions.

[0052] The through hole 73 facilitates the light detected by the detection component 4 to pass through.

[0053] The detection assembly 4 includes an electron microscope 41, a second support frame 42 and a light source 43. The second support frame 42 is fixedly mounted on the top of the partition of the box body 1. The side walls of the second support frame 42 are fixedly connected with the electron microscope 41 and the light source 43 in sequence from top to bottom, and the electron microscope 41 is located below the slide slot 72, and the electron microscope 41 is located above the slide slot 72.

[0054] The slide slot 72 drives the slide to continue to rotate to below the electron microscope 41 of the detection component 4, and the light source 43 emits light. After the light passes through the through hole 73, it supplements the light for the slide, and the electron microscope 41 performs detection and analysis.

[0055] The pick-and-place assembly 3 includes a first cylinder 31, a first support frame 32, a transverse module 33 and a suction cup 34; the first support frame 32 is fixedly installed on the top of the partition of the box body 1, the upper end of the side wall of the first support frame 32 is fixedly connected to the transverse module 33, the driving end of the transverse module 33 is fixedly connected to the first cylinder 31, and the driving end of the first cylinder 31 is fixedly connected to the suction cup 34;

[0056] The first cylinder 31 of the pick-and-place assembly 3 of the slide loading station drives the suction cup 34 to move upward, and the transverse module 33 drives the first cylinder 31 to move downward the piece-by-piece loading assembly 5 of the slide loading station, and the first cylinder 31 drives the suction cup 34 to move downward, and the suction cup 34 contacts the slides in the piece-by-piece loading assembly 5 of the slide loading station, and the suction cup 34 forms a negative pressure to suck the slides in the piece-by-piece loading assembly 5 of the slide loading station, and the first cylinder 31 drives the suction cup 34 to move upward, and the transverse module 33 drives the slide adsorbed by the suction cup 34 to move toward the slide slot 72, and the first cylinder 31 drives the suction cup 34 to move downward, and the suction cup 34 places the slide into the slide slot 72, so as to facilitate the removal of the slides in the piece-by-piece loading assembly 5 of the slide loading station and place them on the equi-angle rotating assembly 7.

[0057] The first cylinder 31 of the pick-and-place assembly 3 of the cover glass loading station drives the suction cup 34 to move upward, and the transverse movement module 33 drives the first cylinder 31 to move downward to the piece-by-piece loading assembly 5 of the cover glass loading station, and the first cylinder 31 drives the suction cup 34 to move downward, and the suction cup 34 contacts the cover glass in the piece-by-piece loading assembly 5 of the cover glass loading station, and the suction cup 34 forms a negative pressure to suck the cover glass in the piece-by-piece loading assembly 5 of the cover glass loading station, and the first cylinder 31 drives the suction cup 34 to move upward, and the transverse movement module 33 drives the cover glass adsorbed by the suction cup 34 to move toward the slide slot 72, and the first cylinder 31 drives the suction cup 34 to move downward, and the suction cup 34 places the cover glass into the slide slot 72, so as to facilitate taking out the cover glass in the piece-by-piece loading assembly 5 of the cover glass loading station and placing it on the slide on the equi-angle rotating assembly 7.

[0058] The first cylinder 31 of the pick-and-place assembly 3 of the waste unloading station drives the suction cup 34 to move upward, and the transverse movement module 33 pushes the suction cup 34 to move toward the slide slot 72. After the suction cup 34 moves to the waste unloading station, the first cylinder 31 drives the suction cup 34 to move downward, and the suction cup 34 moves to contact the waste at the waste unloading station. The suction cup 34 generates negative pressure to suck the waste at the waste unloading station by the suction cup 34. The first cylinder 31 drives the suction cup 34 to move upward, and the transverse movement module 33 drives the waste sucked by the suction cup 34 to move above the cover glass piece by piece loading assembly 6. The air pressure in the suction cup 34 returns to normal pressure, and the waste falls into the cover glass piece by piece loading assembly 6 by gravity, and the cover glass piece by piece loading assembly 6 discharges the waste.

[0059] The piece-by-piece loading assembly 5 comprises a second cylinder 51, a material storage barrel 52, a second groove 53, a push plate 54, a third support frame 55 and a support seat 56. The third support frame 55 is fixedly installed on the top of the partition of the box body 1. The top of the third support frame 55 is fixedly connected with the second cylinder 51 and the support seat 56. The top of the support seat 56 is provided with a second groove 53. The end of the top of the support seat 56 close to the second cylinder 51 is fixedly connected with the material storage barrel 52. The driving end of the second cylinder 51 is fixedly connected with the push plate 54. The outer wall of the push plate 54 is slidably connected with the inner wall of the second groove 53. The top of the push plate 54 is slidably connected with the bottom of the material storage barrel 52. Slides are stored in the material storage barrel 52 at the slide loading station, and cover glasses are stored in the material storage barrel 52 at the cover glass loading station. The end of the support seat 56 away from the second cylinder 51 is located below the corresponding pick-and-place assembly 3.

[0060] There are slides stored in the storage barrel 52 of the slide loading station, and the second cylinder 51 drives the push plate 54 to move toward the second cylinder 51. After the second cylinder 51 drives the end away from the push plate 54 to move past the storage barrel 52, the slides in the storage barrel 52 move downward, and the second cylinder 51 drives the push plate 54 to move toward the support seat 56, and the push plate 54 pushes the bottom slide to move to the end of the second groove 53 away from the second cylinder 51; the first cylinder 31 of the pick-and-place assembly 3 of the slide loading station drives the suction cup 34 to move downward, and the transverse module 33 drives the first cylinder 31 to move downward in the second groove 53 of the slide loading station away from the end of the second cylinder 51, and the second cylinder 51 drives the first cylinder 31 to move downward in the second groove 53 of the slide loading station away from the end of the second cylinder 51. A cylinder 31 drives the suction cup 34 to move downward, and the suction cup 34 contacts the glass slide in the end of the second groove 53 of the glass slide loading station away from the second cylinder 51. The suction cup 34 forms a negative pressure to suck the glass slide in the second groove 53 of the glass slide loading station. The first cylinder 31 drives the suction cup 34 to move upward, and the transverse module 33 drives the glass slide adsorbed by the suction cup 34 to move toward the glass slide slot 72. The first cylinder 31 drives the suction cup 34 to move downward, and the suction cup 34 places the glass slide into the glass slide slot 72, so as to facilitate the removal of the glass slides in the piece-by-piece loading component 5 of the glass slide loading station and place them on the equi-angle rotating component 7, so as to facilitate the loading of the glass slides one by one.

[0061] There are cover glasses stored in the storage barrel 52 of the cover glass loading station, and the second cylinder 51 drives the push plate 54 to move toward the second cylinder 51. After the second cylinder 51 drives the end away from the push plate 54 to move past the storage barrel 52, the cover glasses in the storage barrel 52 move downward, and the second cylinder 51 drives the push plate 54 to move toward the support seat 56, and the push plate 54 pushes the bottom cover glass to move to the end of the second groove 53 away from the second cylinder 51; the first cylinder 31 of the pick-and-place assembly 3 of the cover glass loading station drives the suction cup 34 to move downward, and the transverse module 33 drives the first cylinder 31 to move the second groove 53 of the cover glass loading station away from the end of the second cylinder 51, and the first cylinder 31 drives the suction cup 34 to move downward, and the suction cup 34 and the cover glass loading station The suction cup 34 forms a negative pressure to suck the cover glass in the second groove 53 of the cover glass loading station, and the first cylinder 31 drives the suction cup 34 to move upward, and the transverse module 33 drives the cover glass adsorbed by the suction cup 34 to move toward the slide slot 72, and the first cylinder 31 drives the suction cup 34 to move downward, and the suction cup 34 places the cover glass into the slide slot 72 and covers the cover glass on the slide, so as to facilitate the removal of the cover glass in the piece-by-piece loading component 5 of the cover glass loading station and place it on the cover glass on the equi-angle rotating component 7, so as to facilitate the loading of the cover glass piece by piece.

[0062] The pipetting assembly 8 includes an XYZ-axis drive assembly 81, a fourth mounting frame 82, an electromagnetic lock 83 and a manual pipette 84. The XYZ-axis drive assembly 81 is fixedly installed at the top of the box body 1. The side wall of the Z-axis drive end of the XYZ-axis drive assembly 81 is fixedly connected to the fourth mounting frame 82. The fourth mounting frame 82 is fixedly connected to the electromagnetic lock 83 and the manual pipette 84 from top to bottom in sequence. The electromagnetic lock 83 is located above the manual pipette 84, and the gun head 85 is plugged into the suction end of the manual pipette 84.

[0063] The XYZ axis driving assembly 81 of the pipetting assembly 8 drives the fourth mounting frame 82 to move, and the fourth mounting frame 82 drives the manual pipette 84 to move above the gun tip storage rack 10, and drives the manual pipette 84 to plug with the clean gun tip 85 on the gun tip storage rack 10, and the manual pipette 84 is assembled with the gun tip 85 from the gun tip storage rack 10, and the XYZ axis driving assembly 81 drives the gun tip 85 connected to the manual pipette 84 to move into the semen cup of the semen cup storage rack 9, and the electromagnetic lock 83 is energized to press the manual pipette 84, and the manual pipette 84 After pressing, the gun tip 85 absorbs a certain amount of semen, and the XYZ axis driving component 81 drives the gun tip 85 to move, and the gun tip 85 delivers the absorbed semen to the top of the glass slide of the semen loading station, and the electromagnetic lock 83 is powered off, and the semen in the gun tip 85 falls on the glass slide of the loading station, and then the pipetting component 8 moves the gun tip 85 to the waste gun tip collection component 2, and the waste gun tip collection component 2 and the pipetting component 8 cooperate to collect the gun tip 85, so as to realize the continuous automatic operation of assembling the clean gun tip 85, taking liquid, dropping liquid and collecting the used gun tip 85, and realize automatic sampling;

[0064] The waste gun tip collection assembly 2 includes a collection box 21, a first groove 22 and a horizontal plate 23. The collection box 21 is inserted into the square limit frame 11. The collection box 21 is fixedly connected to the horizontal plate 23 near the top of the semen cup storage rack 9. The end of the horizontal plate 23 away from the semen cup storage rack 9 is provided with a first groove 22. The size of the first groove 22 is smaller than the size of the top of the gun tip 85, and the size of the first groove 22 is larger than the size of the part where the manual pipette 84 is connected to the gun tip 85.

[0065] After the gun tip 85 is used, the XYZ-axis driving assembly 81 drives the gun tip 85 to move to the collection box 21 of the waste gun tip collection assembly 2, and the connection between the manual pipette 84 and the gun tip 85 is inserted into the first groove 22. The gun tip 85 is located below the first groove 22. The XYZ-axis driving assembly 81 drives the manual pipette 84 to move upward, and the horizontal plate 23 pushes the gun tip 85 out from under the manual pipette 84 and collects it into the collection box 21, thereby realizing automatic collection of the used gun tips 85.

[0066] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features may be replaced by equivalents. Such modifications or replacements will not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. An integrated semen sampling and semen reproductive analysis device, comprising a housing (1), characterized in that: An equiangular rotation component (7) for equiangular rotation is connected to the left side of the top of the partition of the box body (1); The box body (1) is equipped with a detection component (4) for detection at the detection station of the equi-angle rotating component (7); The box body (1) is equipped with a pick-and-place assembly (3) for transporting materials at the waste material unloading station, the slide material loading station and the cover glass loading station of the equiangular rotating assembly (7); A cover glass sheet loading assembly (6) is provided below the pick-and-place assembly (3) of the waste material unloading station, and the cover glass sheet loading assembly (6) is fixedly connected to the box body (1); A piece-by-piece loading assembly (5) for feeding piece-by-piece is provided below the pick-and-place assembly (3) at the slide loading station and the cover glass loading station. The piece-by-piece loading assembly (5) is fixedly connected to the partition of the box body (1). The piece-by-piece loading assembly (5) at the slide loading station is used for feeding slides piece-by-piece, and the piece-by-piece loading assembly (5) at the cover glass loading station is used for feeding cover glasses piece-by-piece. A pipetting assembly (8) for semen sampling is connected inside the box body (1), and the left end of the pipetting assembly (8) is located directly above the semen loading station of the equiangular rotating assembly (7); Three groups of square limit frames (11) are provided on the right side of the top of the partition of the box body (1); and the square limit frames (11) are respectively plugged with a semen cup storage rack (9), a gun tip storage rack (10) and a waste gun tip collection assembly (2); semen cups storing semen are plugged into a rectangular array on the semen cup storage rack (9); gun tips (85) are plugged into a rectangular array on the gun tip storage rack (10); and the gun tip storage rack (10) and the waste gun tip collection assembly (2) are located at the rear side of the semen cup storage rack (9), and the waste gun tip collection assembly (2) is located at the right side of the gun tip storage rack (10).

2. The integrated semen sampling and semen reproductive analysis device according to claim 1, characterized in that: The equal-angle rotating assembly (7) comprises a rotating disk (71), a glass slide slot (72), a through hole (73) and an equal-angle driving assembly (74). The equal-angle driving assembly (74) is fixedly mounted on the top of the partition of the box body (1). The driving end of the equal-angle driving assembly (74) is connected to the rotating disk (71). The outer edge of the rotating disk (71) is provided with glass slide slots (72) at equal intervals along the circumferential direction. The rotating disk (71) is provided with through holes (73) at the bottom of the glass slide slots (72), and the bottom of the through holes (73) passes through the bottom of the rotating disk (71).

3. The integrated semen sampling and semen reproductive analysis device according to claim 2, characterized in that: The detection component (4) comprises an electron microscope (41), a second support frame (42) and a light source (43); the second support frame (42) is fixedly mounted on the top of the partition of the box body (1); the side walls of the second support frame (42) are fixedly connected with the electron microscope (41) and the light source (43) in sequence from top to bottom; the electron microscope (41) is located below the slide slot (72); and the electron microscope (41) is located above the slide slot (72).

4. The integrated semen sampling and semen reproductive analysis device according to claim 3, characterized in that: The pick-and-place assembly (3) comprises a first cylinder (31), a first support frame (32), a transverse module (33) and a suction cup (34); the first support frame (32) is fixedly mounted on the top of the partition of the box body (1); the upper end of the side wall of the first support frame (32) is fixedly connected to the transverse module (33); the driving end of the transverse module (33) is fixedly connected to the first cylinder (31); and the driving end of the first cylinder (31) is fixedly connected to the suction cup (34).

5. The integrated semen sampling and semen reproductive analysis device according to claim 4, characterized in that: The piece-by-piece loading assembly (5) comprises a second cylinder (51), a material storage barrel (52), a second groove (53), a push plate (54), a third support frame (55) and a support seat (56). The third support frame (55) is fixedly mounted on the top of the partition of the box body (1). The top of the third support frame (55) is fixedly connected to the second cylinder (51) and the support seat (56). The top of the support seat (56) is provided with a second groove (53). The top of the support seat (56) is fixedly connected to the material storage barrel (52) at the end thereof near the second cylinder (51). The driving end of the second cylinder (51) is fixedly connected to the push plate (54). The outer wall of the push plate (54) is slidably connected to the inner wall of the second groove (53). The top of the push plate (54) is slidably connected to the bottom of the material storage barrel (52). The end of the support seat (56) away from the second cylinder (51) is located below the corresponding pick-and-place assembly (3).

6. The integrated semen sampling and semen reproductive analysis device according to claim 5, characterized in that: The storage cylinder (52) at the slide glass loading station stores a slide glass, and the storage cylinder (52) at the cover glass loading station stores a cover glass.

7. The integrated semen sampling and semen reproductive analysis device according to claim 6, characterized in that: The liquid transfer assembly (8) comprises an XYZ-axis drive assembly (81), a fourth mounting frame (82), an electromagnetic lock (83) and a manual liquid transfer device (84); the XYZ-axis drive assembly (81) is fixedly mounted on the top of the box body (1); the side wall of the Z-axis drive end of the XYZ-axis drive assembly (81) is fixedly connected to the fourth mounting frame (82); the fourth mounting frame (82) is fixedly connected to the electromagnetic lock (83) and the manual liquid transfer device (84) in sequence from top to bottom; the electromagnetic lock (83) is located above the manual liquid transfer device (84); and the gun head (85) is plugged into the suction end of the manual liquid transfer device (84).

8. The integrated semen sampling and semen reproductive analysis device according to claim 7, characterized in that: The waste gun tip collection assembly (2) comprises a collection box (21), a first groove (22) and a transverse plate (23); the collection box (21) is inserted into a square limit frame (11); the collection box (21) is fixedly connected to the transverse plate (23) near the top of the semen cup storage rack (9); and the end of the transverse plate (23) away from the semen cup storage rack (9) is provided with the first groove (22).

9. The integrated semen sampling and semen reproductive analysis device according to claim 8, characterized in that: The size of the first groove (22) is smaller than the size of the top of the gun head (85), and the size of the first groove (22) is larger than the size of the part where the manual pipette (84) and the gun head (85) are connected.

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