A bionic virus construction system based on DNA nanotechnology

Abstract

The present invention discloses a bionic virus construction system based on DNA nanotechnology, comprising: a virus information construction module, a virus information matching module, a bionic virus simulation construction module and a bionic virus construction module; a virus information construction module; a virus information matching module; a bionic virus simulation construction module, which designs a polypeptide sequence and simulates and constructs a virus model based on the current virus key information in the virus information construction module; a bionic virus construction module, which assembles a bionic virus based on the virus model constructed in the virus simulation construction module. The present invention belongs to the field of bionic virus construction technology, and the technical effect achieved is: the present invention can match the information of the current virus with the information of the previous virus, and only screen after the similarity meets the standard, which can reduce a lot of screening time, and can integrate a lot of previous vaccine information to provide a reference for virus construction, and can also perform virtual simulation to reduce experimental time.

Description

Technical Field

[0001] The present invention relates to the technical field of bionic virus construction, and in particular to a bionic virus construction system based on DNA nanotechnology. Background Art

[0002] Biomimetic virus construction refers to the design and manufacture of inactive virus-like particles (VLPs) with similar properties by simulating the structural characteristics and functional mechanisms of viruses in nature. These particles are usually made of biocompatible materials and can perform specific biological functions without causing infection.

[0003] Since its founding by Nadrian Seeman in 1982, DNA nanotechnology has experienced 40 years of rapid development and has found widespread applications in biology, medicine, materials science, chemistry, and other fields. The emergence of DNA origami in 2006 reduced the design difficulty of DNA nanostructures and improved their programmability, significantly expanding the application of DNA nanotechnology. Biomimetic viruses constructed using DNA nanotechnology closely resemble real viruses but lack viral genetic material and cannot replicate. This allows them to effectively stimulate the immune system.

[0004] Currently, the construction of bionic viruses is completed based on existing materials, and then implemented on experimental organisms to observe the effects. Finding matching bionic virus materials often requires consulting a large amount of information and data, which takes a lot of time and affects the matching efficiency. At the same time, specific application data can only be obtained after the experiment, resulting in a long construction cycle. Summary of the Invention

[0005] To this end, the present invention provides a bionic virus construction system based on DNA nanotechnology to solve the above-mentioned problems in the prior art.

[0006] In order to achieve the above object, the present invention provides the following technical solutions:

[0007] A bionic virus construction system based on DNA nanotechnology, comprising:

[0008] Virus information building module, virus information matching module, bionic virus simulation building module and bionic virus building module;

[0009] Virus information building module to obtain current virus key information;

[0010] The virus information matching module matches the historical virus information in the module based on the current virus key information in the virus information construction module. When the matching similarity is greater than 80%, the module obtains the historical virus information that meets the similarity standard and provides the previous vaccine information. At the same time, it extracts the comparative information of the current virus DNA model and the historical virus DNA model.

[0011] The bionic virus simulation construction module designs the peptide sequence and simulates the construction of the virus model based on the current virus key information in the virus information construction module;

[0012] The bionic virus building module assembles bionic viruses according to the virus model constructed in the virus simulation building module.

[0013] Furthermore, current key information about the virus includes the viral genome sequence, the characteristics of its structural proteins, and the mechanism of interaction between the virus and host cells.

[0014] Furthermore, the specific steps of simulating and constructing a virus model include:

[0015] S1: Vector selection: Based on the key information of the virus, select the corresponding nanocarrier to provide corresponding targeted delivery and functional enhancement;

[0016] S2: Framework nucleic acid construction: Using DNA origami technology, the staple chain is extended to the corresponding sequence at a specific position to construct the corresponding framework nucleic acid;

[0017] S3: Assemble virtual bionic cell components and select identical or similar DNA or RNA sequences based on previous vaccine information;

[0018] S4: Simulate the activities of virtual bionic cell components and use known data to simulate the movement path and function of the current virtual bionic cell components.

[0019] Furthermore, nanocarriers include organic nanocarriers, inorganic nanocarriers, and biomimetic carriers.

[0020] Furthermore, biomimetic carriers also include cell membrane nanovesicles:

[0021] Cell membrane nanovesicles include: natural cell or cell membrane-derived nanovesicles, exosomes, and small microvesicles.

[0022] Furthermore, the biomimetic carrier can also release submicron particles from cells in situ.

[0023] Furthermore, when the simulated activity of the virtual bionic cell element does not meet the expected effect, the process returns to S1 to reconstruct the virus.

[0024] Furthermore, the known data include current vector function data, current framework nucleic acid function data, and patient's in vivo biological environment data.

[0025] Furthermore, molecular walkers can be added to the bionic virus building blocks.

[0026] The present invention has the following advantages:

[0027] The present invention can match the information of the current virus with the information of previous viruses, and only screen when the similarity meets the standard. This can reduce a lot of screening time, and can integrate a lot of past vaccine information to provide a reference for virus construction. It can also perform virtual simulation to reduce experimental time. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 This is a module connection diagram of a bionic virus construction system based on DNA nanotechnology provided by the present invention.

[0029] Figure 2 This is a workflow diagram of a bionic virus simulation construction module of a bionic virus construction system based on DNA nanotechnology provided by the present invention. DETAILED DESCRIPTION

[0030] The following describes the implementation of the present invention using specific embodiments. Those skilled in the art will readily understand the other advantages and benefits of the present invention from the disclosure herein. Obviously, the embodiments described are only a portion of the present invention, not all of it. All other embodiments derived by persons of ordinary skill in the art based on the embodiments of the present invention without inventive effort are intended to fall within the scope of protection of the present invention.

[0031] like Figures 1 to 2 As shown, the present invention provides a bionic virus construction system based on DNA nanotechnology, including: a virus information construction module, a virus information matching module, a bionic virus simulation construction module and a bionic virus construction module.

[0032] Virus information construction module, which obtains key information about the current virus, including the viral genome sequence, the characteristics of structural proteins, and the mechanism of interaction between the virus and host cells;

[0033] The virus information matching module matches the historical virus information in the module based on the current virus key information in the virus information construction module. When the matching similarity is greater than 80%, the module obtains the historical virus information that meets the similarity standard and provides the previous vaccine information. At the same time, it extracts the comparative information of the current virus DNA model and the historical virus DNA model.

[0034] The bionic virus simulation construction module designs peptide sequences based on the current virus key information in the virus information construction module and simulates and constructs a virus model, specifically including:

[0035] S1: Carrier selection: Based on the key information of the virus, select the corresponding nanocarrier to provide corresponding targeted delivery and function enhancement. Nanocarriers include: organic nanocarriers, inorganic nanocarriers, and biomimetic carriers;

[0036] For example, lecithin-based nanoliposomes, as organic nanocarriers, can be delivered to Cas9 / sgRNA RNPs, with the characteristics of high biocompatibility, low cytotoxicity, and high solution stability;

[0037] Gold nanoparticles, as inorganic nanocarriers, can be delivered to Cas9 / sgRNA RNP, DNA, and Cas9-sgRNA plasmids, and have unique optical and structural properties that can serve as a versatile platform for gene delivery;

[0038] Virus-like particles, as biomimetic nanocarriers, can be delivered to Cas9 / sgRNA RNP, achieving instantaneous and rapid delivery while reducing off-target rates;

[0039] Alternatively, cell membrane nanovesicles can be selected. Genetically engineered cell membrane nanovesicles exhibit better targeting and immunotherapy capabilities, such as natural cell or cell membrane-derived nanovesicles, exosomes, small microvesicles, and submicron particles released by cells in situ.

[0040] S2: Framework nucleic acid construction: Using DNA origami technology, the staple chains at specific positions of the origami structure are extended to form corresponding sequences to construct corresponding framework nucleic acids. The recognition and binding of target nucleic acid molecules (including DNA and RNA) in the solution can be achieved through molecular hybridization, and the nucleic acid properties of the framework nucleic acid are constructed to provide conditions for the assembly of target molecule nucleic acid fragments. The structures of the framework nucleic acid include but are not limited to triangles, rectangles, rods, nanotubes, nanocages, tetrahedrons, cubes, octahedrons, and icosahedrons.

[0041] S3: Assemble virtual bionic cell components and select identical or similar DNA or RNA sequences based on previous vaccine information;

[0042] S4: Simulate the activity of virtual bionic cell components. Use known data to simulate the movement path and function of the current virtual bionic cell components. If the simulated activity of the virtual bionic cell components does not meet the expected effect, return to S1 to reconstruct the virus. The known data include the current vector function data, the current framework nucleic acid function data, and the patient's in vivo biological environment data.

[0043] The bionic virus building module assembles bionic viruses according to the virus model constructed in the virus simulation building module.

[0044] A molecular walker can also be installed so that the bionic virus can move autonomously on the molecular track after the molecular walker is installed. The molecular walker is also constructed using DNA origami technology.

Claims

1. A bionic virus construction system based on DNA nanotechnology, characterized in that: include: Virus information building module, virus information matching module, bionic virus simulation building module and bionic virus building module; Virus information building module to obtain current virus key information; The virus information matching module matches the historical virus information in the module based on the current virus key information in the virus information construction module. When the matching similarity reaches more than 80%, it obtains the historical virus information that meets the similarity standard and provides the previous vaccine information. At the same time, it extracts the comparative information of the current virus DNA model and the historical virus DNA model. The bionic virus simulation construction module designs a polypeptide sequence based on the current virus key information and the comparison information in the virus information construction module, and simulates and constructs a virus model; A bionic virus building module, which assembles bionic viruses based on the virus model constructed in the virus simulation building module; The specific steps for simulating and building a virus model include: S1: Vector selection: Based on the key information of the virus, select the corresponding nanocarrier to provide corresponding targeted delivery and functional enhancement; S2: Framework nucleic acid construction: Using DNA origami technology, the staple chain is extended to the corresponding sequence at a specific position to construct the corresponding framework nucleic acid; S3: Assemble virtual bionic cell components and select identical or similar DNA or RNA sequences based on previous vaccine information; S4: Simulate the activities of virtual bionic cell components and use known data to simulate the movement path and function of the current virtual bionic cell components.

2. The bionic virus construction system based on DNA nanotechnology according to claim 1, characterized in that: Current key information about the virus includes the viral genome sequence, the characteristics of its structural proteins, and the mechanism of interaction between the virus and host cells.

3. The bionic virus construction system based on DNA nanotechnology according to claim 1, characterized in that: Nanocarriers include: organic nanocarriers, inorganic nanocarriers, and biomimetic carriers.

4. The bionic virus construction system based on DNA nanotechnology according to claim 3, characterized in that: Biomimetic carriers also include cell membrane nanovesicles: Cell membrane nanovesicles include: natural cell or cell membrane-derived nanovesicles, exosomes, and small microvesicles.

5. The bionic virus construction system based on DNA nanotechnology according to claim 4, characterized in that: Biomimetic carriers can also release submicron particles from cells in situ.

6. The bionic virus construction system based on DNA nanotechnology according to claim 1, characterized in that: When the simulated activity of the virtual bionic cell element does not meet the expected effect, return to S1 to reconstruct the virus.

7. The bionic virus construction system based on DNA nanotechnology according to claim 1, characterized in that: Known data include current vector function data, current framework nucleic acid function data, and patient in vivo biological environment data.

8. The bionic virus construction system based on DNA nanotechnology according to claim 1, characterized in that: Adding molecular walkers to biomimetic virus building blocks.

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