Broussonetia papyrifera source ecological pesticide or growth promoting agent and preparation process thereof

Abstract

The invention discloses a broussonetia papyrifera source ecological pesticide or growth promoting agent and a preparation process thereof.The broussonetia papyrifera leaf extract is prepared from broussonetia papyrifera leaves, ethyl alcohol, dichloromethane, ethyl acetate and n-butyl alcohol, and the preparation process of the broussonetia papyrifera source ecological pesticide or growth promoting agent comprises the following steps that broussonetia papyrifera leaf raw materials are prepared; extracting a paper mulberry leaf raw material by using alcohol, adding petroleum ether to extract the solution collected in the step 2, collecting the obtained solution, adding dichloromethane to the solution collected in the step 3, adding ethyl acetate to the solution collected in the step 4, adding n-butyl alcohol to the solution collected in the step 5, collecting the obtained extract, and drying to obtain the paper mulberry leaf extract. Purifying the final extract of the broussonetia papyrifera leaves to prepare a pesticide or a growth promoting agent. According to the tree source ecological pesticide or the growth promoting agent and the preparation process thereof, the pesticide or the growth promoting agent is prepared from the extracts of the paper mulberry leaves, so that the germination of appressorium of pyricularia grisea spores of rice blast can be inhibited, the effect of promoting the growth of rice is achieved, and the yield of the rice is increased.

Description

Technical Field

[0001] The invention relates to the technical field of pesticide and growth promoter preparation, in particular to a paper mulberry source ecological pesticide or growth promoter and a preparation process thereof. Background Art

[0002] Rice blast is one of the important diseases of rice, which can cause a significant reduction in yield. In severe cases, the reduction is 40% to 50%, or even no grain is harvested. It occurs evenly in rice-growing areas around the world. This disease occurs in all places, with the leaf and node parts being the most common. After the disease occurs, it can cause different degrees of reduction in yield, especially the early and severe occurrence of ear neck blast or node blast, which can cause white ears and even total loss of yield. In recent years, the annual occurrence area of ​​rice blast in Guangdong is no less than 500,000 mu, and there is an increasing trend year by year. Local outbreaks are not uncommon. Rice blast may occur in any year and any season within the province. The prevention and control methods of rice blast mainly include the selection of disease-resistant varieties, the cultivation of high-quality seedlings, fertilizer and water management technical measures, the strengthening of field management, the implementation of prevention and control measures, and chemical control. After the epidemic of rice blast, it is necessary to effectively grasp the symptoms, laws and causes of rice blast, and take scientific and feasible technical measures for the prevention and control of rice blast in combination with the planting situation to avoid losses in rice yield and quality.

[0003] Broussonetia papyrifera is a tall tree or shrub-like plant of the genus Broussonetia in the Moraceae family. Its twigs are densely covered with gray coarse hairs. The leaves are broadly ovate or oblong-ovate, with a pointed tip and a nearly heart-shaped, flat or rounded base with coarse serrations. Broussonetia papyrifera is produced in various parts of China, and is also distributed in countries such as northern South Asia, Southeast Asia, and East Asia. Broussonetia papyrifera is light-loving, highly adaptable, and resistant to drought and barrenness. It can also grow by the water and grows mostly in limestone mountains. It can also grow in acidic and neutral soils. The general propagation method of Broussonetia papyrifera is cutting and sowing. The bark, leaves, and seeds of Broussonetia papyrifera can be used as medicine. The bark tastes sweet and is neutral in nature. It has the effects of diuresis, swelling, wind and dampness. The leaves taste sweet and are cool in nature. They have the effects of clearing heat, cooling blood, dampness and insecticide. The seeds taste sweet and are cold in nature. They have the effects of strengthening the kidney and tendons, improving eyesight and diuresis. The bast fiber of Broussonetia papyrifera can be used to make paper, the fruit can be eaten raw, or brewed, and the young leaves can be used as feed for pigs. In terms of environmental protection, Paulownia is suitable for greening mining areas and barren mountain slopes, and can also be selected as a garden shade tree and protective forest.

[0004] In view of the advantages and efficacy of paper mulberry leaves, the existing technology is mostly used in agriculture to prepare feed by extracting paper mulberry leaves, such as a paper mulberry feed and its preparation method with publication number CN113598268A, and its technical scheme is: the raw materials of the paper mulberry feed include: 40% to 60% paper mulberry leaves, 5% to 25% corn flour, 5% to 10% soybean meal, 8% to 15% bran, 5% stone powder, 5% premix, 2% enzyme preparation, and 3% to 10% beer. The paper mulberry feed efficiently utilizes the rich protein in paper mulberry and reduces the amount of feed grain. At the same time, adding beer in the feed production process can effectively improve the disease resistance of livestock and poultry; and it can overcome the problem that similar addition of distiller's grains can easily cause feed mold.

[0005] In the prior art, there is a method for extracting paper mulberry, such as an extraction method and application of a paper mulberry extract with publication number CN114668792A, and its technical scheme is: the method is to obtain the paper mulberry extract by drying and crushing the branches and leaves of paper mulberry, then soaking them in an ethanol solution, filtering, concentrating and drying them; the paper mulberry extract of the present application is a natural plant component, which has the advantages of not producing drug resistance and no residue, and has broad application prospects in disease treatment; the paper mulberry extract of the present invention can effectively inhibit the replication of PEDV and SADS-CoV in Vero E6 cells, and when the concentration of the paper mulberry extract is greater than 2.5 mg / ml, it can completely inhibit the proliferation of PEDV and SADS-CoV, and can be widely used in the prevention and treatment of porcine epidemic diarrhea. The present invention is the first to discover that the paper mulberry extract has the activity of effectively inhibiting the replication of porcine diarrhea virus, and has good application value and market prospects.

[0006] However, the technology of using extracts of the components of the paper mulberry leaves to enhance the antibacterial effect, especially for inhibiting the germination of the appressorium spores of rice blast fungus, needs to be improved. The preparation process of pesticides and growth promoters using extracts of the paper mulberry leaves needs to be further studied.

[0007] In view of this, we conducted in-depth research on the above issues, which led to the present case. Summary of the invention

[0008] The object of the present invention is to provide an ecological pesticide or growth promoter derived from paper mulberry and its preparation process, so as to solve the problem proposed in the above background technology of inhibiting the germination of appressorium spores of rice blast fungus by paper mulberry leaf extract.

[0009] To achieve the above object, the present invention provides the following technical solutions:

[0010] A paper mulberry source ecological pesticide or growth promoter and a preparation process thereof, wherein the preparation raw materials of the pesticide or growth promoter include paper mulberry leaf extract, and the preparation raw materials of the paper mulberry leaf extract include paper mulberry leaves, alcohol, dichloromethane, ethyl acetate and n-butanol, and the preparation process of the paper mulberry source ecological pesticide or growth promoter comprises the following steps:

[0011] Step 1, prepare the raw materials of paper mulberry leaves;

[0012] Step 2, using alcohol to extract the paper mulberry leaf raw material and collecting the solution;

[0013] Step 3, adding petroleum ether to extract the solution collected in step 2, and collecting the resulting solution;

[0014] Step 4, adding dichloromethane to the solution collected in step 3, and collecting the resulting solution;

[0015] Step 5, adding ethyl acetate to the solution collected in step 4, and collecting the resulting solution;

[0016] Step 6, adding n-butanol to the solution collected in step 5, and collecting the resulting extract;

[0017] Step seven, purifying the final extract of the paper mulberry leaves to prepare pesticides or growth promoters.

[0018] Preferably, the specific steps of step one are: collecting an appropriate amount of paper mulberry leaves, drying them in the sun, and crushing the dried paper mulberry leaves with a grinder to obtain dried paper mulberry leaf pieces.

[0019] The above technical solution is used to dry the leaves of the paper mulberry tree to prevent the moisture in the leaves from affecting the extraction effect. The moisture inside the leaves will affect the combination of organic reagents and pigment molecules such as chlorophyll. Drying and crushing the leaves can effectively improve the extraction efficiency.

[0020] Preferably, the specific steps of step 2 are: mixing the dried paper mulberry leaves obtained in step 1 with 75% ethanol solution, and the mixing ratio of the dried paper mulberry leaves and 75% ethanol solution is 1:1, the number of extractions is 3 times, the extraction time is 24 hours, and the obtained solution is collected.

[0021] By adopting the above technical scheme, the chlorophyll in the paper mulberry leaves is insoluble in water but can be dissolved in ethanol solution. At this time, the chlorophyll in the paper mulberry leaves is dissolved, which is convenient for subsequent extraction.

[0022] Preferably, the specific steps of step three are: adding petroleum ether to the solution collected in step two, extracting and hydrolyzing the solution with petroleum ether, and filtering impurities at the same time.

[0023] The above technical scheme is adopted, through the petroleum ether extraction method, the cost is low, and it has the characteristics of low polarity, strong volatility, low boiling point, etc., so it has a good effect in extraction. It can dissolve and extract various organic compounds well, including oils, pigments, resins, natural products and chemical synthetics.

[0024] Preferably, the specific steps of step three are: using high performance liquid chromatography to determine the flavonoid compounds in the solution obtained by extraction, and performing reduced pressure concentration and chromatographic separation on the extraction solution to obtain a petroleum ether phase and an aqueous phase.

[0025] The above technical solution is used to detect flavonoid compounds, that is, to accurately test the components and contents therein, to ensure the repeatability of the data and results, and to help improve the extraction efficiency of petroleum ether and obtain the extracted aqueous solution.

[0026] Preferably, the specific steps of step 4 are: adding dichloromethane to the aqueous solution obtained in step 3, separating the fat-soluble substances in the aqueous solution, transferring the aqueous phase to the organic phase, and obtaining a dichloromethane phase and an aqueous phase.

[0027] By adopting the above technical scheme, adding dichloromethane can form a co-solution with a variety of substances. Adding dichloromethane to water can separate the components in the mixture. In a mixture of fat-soluble substances and water-soluble substances, dichloromethane can separate the fat-soluble substances and transfer them from the aqueous phase to the organic phase. Dichloromethane can extract organic matter in water and has a purification effect.

[0028] Preferably, the specific steps of step five are: placing the aqueous solution collected in step four in an extraction separation device, and adding ethyl acetate in the extraction separation device for extraction to obtain an ethyl acetate phase and an aqueous phase.

[0029] Using the above technical solution, ethyl acetate is a relatively strong polar solvent and has good solubility for polar molecules. Therefore, in organic chemistry and biochemistry research, it is very suitable for separating and purifying polar compounds. Ethyl acetate, as a mobile phase, has good heat resistance and suitable polarity.

[0030] Preferably, the specific steps of step six are: adding n-butanol to the aqueous solution collected in step five, fully stirring and mixing the aqueous solution and n-butanol for 30 minutes, standing for 30 minutes, repeating three times and then standing, and finally separating the water layer to obtain n-butanol and an aqueous phase.

[0031] By adopting the above technical scheme, n-butanol can extract hydrophilic components from the water extract because n-butanol is a relatively strong solvent that can dissolve certain hydrophilic components, while water is a relatively weak solvent that cannot dissolve certain hydrophilic components. Therefore, adding an appropriate amount of n-butanol to the water extract can separate the hydrophilic components and extract them from it.

[0032] Preferably, the specific steps of step seven are: adding the extract solution obtained in step six to the preparation process of the pesticide or growth promoter, stirring and mixing, and then filtering to obtain the pesticide or growth promoter containing the paper mulberry extract.

[0033] By adopting the above technical scheme, the extract of paper mulberry leaves is used to prepare pesticides or growth promoters, and its antibacterial ability can be tested through a broad-spectrum antibacterial experiment. At the same time, the pesticide made can inhibit the germination of the appressed cells of the rice blast fungus spores of rice blast disease, thereby promoting the growth of rice and increasing rice yield.

[0034] Compared with the prior art, the invention has the following beneficial effects: the paper mulberry source ecological pesticide or growth promoter and its preparation process,

[0035] 1. Use dichloromethane to extract the paper mulberry leaf solution, add dichloromethane, separate the fat-soluble substances in the aqueous solution, transfer the aqueous phase to the organic phase, and obtain a dichloromethane phase and an aqueous phase. Adding dichloromethane can form a co-solution with a variety of substances. Adding dichloromethane to water can separate the components in the mixture. In a mixture of fat-soluble substances and water-soluble substances, dichloromethane can separate the fat-soluble substances and transfer them from the aqueous phase to the organic phase. Dichloromethane can extract organic matter in the water and has a purification effect at the same time;

[0036] 2. Use ethyl acetate to extract the paper mulberry leaf solution, place the collected aqueous solution in an extraction and separation device, and add ethyl acetate in the extraction and separation device for extraction to obtain ethyl acetate phase and aqueous phase. Ethyl acetate is a relatively strong polar solvent and has good solubility for polar molecules. Therefore, in organic chemistry and biochemistry research, it is very suitable for separating and purifying polar compounds. Ethyl acetate, as a mobile phase, has good heat resistance and suitable polarity;

[0037] 3. Use n-butanol to extract the paper mulberry leaf solution, add n-butanol to the collected aqueous solution, stir and mix the aqueous solution and n-butanol thoroughly, stir for 30 minutes, let stand for 30 minutes, repeat three times and let stand, finally separate the water layer to obtain n-butanol and water phase. n-butanol can extract hydrophilic components from the water extract because n-butanol is a relatively strong solvent that can dissolve some hydrophilic components, while water is a relatively weak solvent that cannot dissolve some hydrophilic components. Therefore, adding an appropriate amount of n-butanol to the water extract can separate the hydrophilic components and extract them from them;

[0038] 4. Prepare pesticides or growth promoters using the extract of paper mulberry leaves. Add the extract solution obtained in step 6 to the preparation process of the pesticide or growth promoter, stir and mix, and then filter to obtain the pesticide or growth promoter containing paper mulberry extract. The antibacterial ability of the pesticide or growth promoter prepared using the extract of paper mulberry leaves can be tested through a broad-spectrum antibacterial experiment. At the same time, the pesticide made into it can inhibit the germination of the appressed cells of the spores of rice blast fungus, thereby promoting the growth of rice and increasing the yield of rice. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] Figure 1 This is a schematic diagram of the broad-spectrum antibacterial experiment of the present invention;

[0040] Figure 2 This is a comparison diagram of the broad-spectrum antibacterial experiment of the present invention;

[0041] Figure 3 This is a bar graph of the broad-spectrum antibacterial experiment of the present invention;

[0042] Figure 4 Experimental study on the inhibition of appressorium germination of spores of rice blast fungus by crude extract of paper mulberry leaves of the present invention

[0043] Microscope runs through the comparison chart;

[0044] Figure 5 It is a schematic diagram comparing the spore germination rate and the spore attachment cell formation rate of the present invention;

[0045] Figure 6 This is a comparison diagram of the actual objects of the rice trichoderma experiment of the present invention;

[0046] Figure 7 This is a comparative bar graph of rice treated with the crude extract of paper mulberry in the rice trichoderma experiment of the present invention;

[0047] Figure 8 This is a bar graph comparing the biomass of sheath blight in the rice trichoderma experiment of the present invention;

[0048] Fig. 9 This is a comparison chart of the rice growth promotion experimental results of the present invention. DETAILED DESCRIPTION

[0049] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0050] A paper mulberry source ecological pesticide or growth promoter and a preparation process thereof, wherein the preparation raw materials of the pesticide or growth promoter include paper mulberry leaf extract, and the preparation raw materials of the paper mulberry leaf extract include paper mulberry leaves, alcohol, dichloromethane, ethyl acetate and n-butanol, and the preparation process of the paper mulberry source ecological pesticide or growth promoter includes the following steps:

[0051] Step 1, prepare the raw materials of paper mulberry leaves;

[0052] Step 2, using alcohol to extract the paper mulberry leaf raw material and collecting the solution;

[0053] Step 3, adding petroleum ether to extract the solution collected in step 2, and collecting the resulting solution;

[0054] Step 4, adding dichloromethane to the solution collected in step 3, and collecting the resulting solution;

[0055] Step 5, adding ethyl acetate to the solution collected in step 4, and collecting the resulting solution;

[0056] Step 6, adding n-butanol to the solution collected in step 5, and collecting the resulting extract;

[0057] Step seven, purifying the final extract of the paper mulberry leaves to prepare pesticides or growth promoters.

[0058] The specific steps of step one are: collecting an appropriate amount of paper mulberry leaves, drying them in the sun, crushing the dried paper mulberry leaves with a crusher, and obtaining paper mulberry leaf pieces. Drying the paper mulberry leaves in the sun prevents the moisture in the leaves from affecting the extraction effect. The moisture inside the leaves will affect the combination of organic reagents and pigment molecules such as chlorophyll. Drying and crushing the leaves effectively improves the extraction efficiency.

[0059] The specific steps of step 2 are: mixing the dried leaves of the paper mulberry obtained in step 1 with a 75% ethanol solution, and the mixing ratio of the dried leaves of the paper mulberry and the 75% ethanol solution is 1:1, the number of extractions is 3 times, the extraction time is 24 hours, and the resulting solution is collected. The chlorophyll in the paper mulberry leaves is insoluble in water but soluble in an ethanol solution. At this time, the chlorophyll in the paper mulberry leaves is dissolved, which is convenient for subsequent extraction.

[0060] The specific steps of step three are: adding petroleum ether to the solution collected in step two, the solution is extracted and hydrolyzed by petroleum ether, and impurities are filtered at the same time. The specific steps of step three are: using high performance liquid chromatography to determine the flavonoids of the solution obtained by extraction, and the extraction solution is concentrated under reduced pressure and chromatographic separation to obtain a petroleum ether phase and an aqueous phase. Through the petroleum ether extraction method, its cost is relatively low, and it has the characteristics of low polarity, high volatility, and low boiling point, so it has a good effect in extraction, and it can dissolve and extract various organic compounds, including oils, pigments, resins, natural products, and chemical synthesis. The detection of flavonoids is to accurately test the components and contents therein, ensure the repeatability of its data and results, help improve the extraction efficiency of petroleum ether, and obtain the aqueous solution after extraction.

[0061] The specific steps of step 4 are: adding dichloromethane to the aqueous solution obtained in step 3, separating the fat-soluble substances in the aqueous solution, transferring the aqueous phase to the organic phase, and obtaining a dichloromethane phase and an aqueous phase. The specific steps of step 5 are: placing the aqueous solution collected in step 4 in an extraction separation device, and adding ethyl acetate in the extraction separation device for extraction to obtain an ethyl acetate phase and an aqueous phase. The specific steps of step 6 are: adding n-butanol to the aqueous solution collected in step 5, stirring and mixing the aqueous solution and n-butanol, stirring for 30 minutes, standing for 30 minutes, repeating three times and standing, and finally separating the water layer to obtain n-butanol and an aqueous phase. Adding dichloromethane can form a co-solution with a variety of substances, adding dichloromethane to water can separate the components in the mixture, in a mixture of fat-soluble substances and water-soluble substances, dichloromethane can separate fat-soluble substances, transfer them from the aqueous phase to the organic phase, and dichloromethane can extract organic matter in water, and has a purification effect at the same time. Ethyl acetate is a relatively strong polar solvent with good solubility for polar molecules. Therefore, it is very suitable for separating and purifying polar compounds in organic chemistry and biochemistry research. As a mobile phase, ethyl acetate has good heat resistance and suitable polarity. n-Butanol can extract hydrophilic components from water extracts because n-Butanol is a relatively strong solvent that can dissolve certain hydrophilic components, while water is a relatively weak solvent that cannot dissolve certain hydrophilic components. Therefore, adding an appropriate amount of n-Butanol to the water extract can separate the hydrophilic components and extract them from it.

[0062] The specific steps of step 7 are: adding the extract solution obtained in step 6 to the preparation process of the pesticide or growth promoter, stirring and mixing, and filtering to obtain the pesticide or growth promoter containing the paper mulberry extract. The use of the extract of paper mulberry leaves to prepare the pesticide or growth promoter can detect its antibacterial ability through a broad-spectrum antibacterial experiment, and the pesticide can inhibit the germination of the appressorium spores of the rice blast fungus, thereby promoting the growth of rice and increasing the yield of rice.

[0063] Experiment 1: Broad-spectrum antibacterial experiment

[0064] Antibacterial rate: (Mock-treatment diameter) / Mock×100%

[0065] The antibacterial rate chart of the broad-spectrum antibacterial experiment is attached to the instruction manual Figure 1 , Instruction Manual Figure 2 And the instruction manual Figure 3 ;

[0066] Experiment 2: Experiment on inhibition of appressorium germination of spores of rice blast fungus by crude extract of paper mulberry leaves

[0067] The experimental results refer to the instructions attached Figure 4 And the instruction manual Figure 5 It can be seen that 1X Broussonetia papyrifera crude extract significantly inhibited spore germination and the formation of appressorium, 5X caused mycelial deformity and inhibited the formation of appressorium, and the effect weakened with increasing dilution multiples;

[0068] Experiment 3: Rice Bacteria Experiment

[0069] Reference manual for the effect of dripping bacteria Figure 6 , Instruction Manual Figure 7 And the instruction manual Figure 8 , the biomass of Rhizoctonia solani was significantly reduced, which was similar to the results of the drip bacteria experiment;

[0070] Experiment 4: Rice growth promotion experiment

[0071] Rice growth promotion information reference manual Fig. 9 The crude extract of Broussonetia papyrifera leaves will not obviously restrict the growth of rice, but also has a certain promoting effect.

[0072] The contents not described in detail in this specification belong to the prior art known to professional and technical personnel in this field.

[0073] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A paper mulberry source ecological pesticide or growth promoter and its preparation process, characterized in that: The preparation raw materials of the pesticide or growth promoter include paper mulberry leaf extract, and the preparation raw materials of paper mulberry leaf extract include paper mulberry leaf, alcohol, dichloromethane, ethyl acetate and n-butanol. The preparation process of the paper mulberry source ecological pesticide or growth promoter includes the following steps: Step 1, prepare the raw materials of paper mulberry leaves; Step 2, using alcohol to extract the paper mulberry leaf raw material and collecting the solution; Step 3, adding petroleum ether to extract the solution collected in step 2, and collecting the resulting solution; Step 4, adding dichloromethane to the solution collected in step 3, and collecting the resulting solution; Step 5, adding ethyl acetate to the solution collected in step 4, and collecting the resulting solution; Step 6, adding n-butanol to the solution collected in step 5, and collecting the resulting extract; Step seven, purifying the final extract of the paper mulberry leaves to prepare pesticides or growth promoters.

2. The paper mulberry-derived ecological pesticide or growth promoter and its preparation process according to claim 1, characterized in that: The specific steps of step one are: collecting an appropriate amount of paper mulberry leaves, drying them in the sun, and crushing the dried paper mulberry leaves with a grinder to obtain dried paper mulberry leaf pieces.

3. The paper mulberry-derived ecological pesticide or growth promoter and its preparation process according to claim 1, characterized in that: The specific steps of step 2 are: mixing the dried paper mulberry leaves obtained in step 1 with 75% ethanol solution, and the mixing ratio of the dried paper mulberry leaves and 75% ethanol solution is 1:1, the extraction times are 3 times, the extraction time is 24 hours, and the obtained solution is collected.

4. The paper mulberry-derived ecological pesticide or growth promoter and its preparation process according to claim 1, characterized in that: The specific steps of step three are: adding petroleum ether to the solution collected in step two, extracting and hydrolyzing the solution with petroleum ether, and filtering impurities at the same time.

5. The paper mulberry-derived ecological pesticide or growth promoter and its preparation process according to claim 4, characterized in that: The specific steps of step three are: using high performance liquid chromatography to determine the flavonoid compounds in the solution obtained by extraction, and performing reduced pressure concentration and chromatographic separation on the extraction solution to obtain a petroleum ether phase and an aqueous phase.

6. The paper mulberry-derived ecological pesticide or growth promoter and its preparation process according to claim 1, characterized in that: The specific steps of step 4 are: adding dichloromethane to the aqueous solution obtained in step 3, separating the fat-soluble substances in the aqueous solution, transferring the aqueous phase to the organic phase, and obtaining a dichloromethane phase and an aqueous phase.

7. The paper mulberry-derived ecological pesticide or growth promoter and its preparation process according to claim 1, characterized in that: The specific steps of step five are: placing the aqueous solution collected in step four in an extraction separation device, and adding ethyl acetate in the extraction separation device for extraction to obtain an ethyl acetate phase and an aqueous phase.

8. The paper mulberry-derived ecological pesticide or growth promoter and its preparation process according to claim 1, characterized in that: The specific steps of step six are: adding n-butanol to the aqueous solution collected in step five, fully stirring and mixing the aqueous solution and n-butanol for 30 minutes, standing for 30 minutes, repeating three times and then standing, and finally separating the water layer to obtain n-butanol and an aqueous phase.

9. The paper mulberry-derived ecological pesticide or growth promoter and its preparation process according to claim 1, characterized in that: The specific steps of step seven are: adding the extract solution obtained in step six to the preparation process of the pesticide or growth promoter, stirring and mixing, and then filtering to obtain the pesticide or growth promoter containing the paper mulberry extract.

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