Preparation method of infant rice flour capable of reducing infant allergy symptoms

Abstract

The invention relates to the technical field of infant food, in particular to a preparation method of infant rice flour capable of reducing infant allergy symptoms. The method comprises the following steps: step 1, sorting and cleaning rice raw materials: selecting GABA-rich germinated rice, cleaning, removing silt, impurities, bran layers, surface microorganisms and pesticide residues, and taking 94-96 parts of GABA-rich germinated rice, 1-2 parts of a mineral nutrient bag, 1-2 parts of a vitamin nutrient bag, 1-2 parts of fructo-oligosaccharide and 1-2 parts of coconut oil for later use; step 2, high static pressure pretreatment; and step 3, high-humidity steam rapid water absorption treatment. Step 4, shearing and slurrying treatment: adding the raw materials treated in the step 3 into a pulping machine, sieving slurry obtained by slurrying, then adding a mineral nutrient bag, a vitamin nutrient bag, fructo-oligosaccharide and coconut oil, and uniformly mixing to obtain rice milk; and 5, roller drying treatment. And step 6, crushing the crushed and sieved infant rice flakes, and sieving the crushed and sieved infant rice flakes with a 5mm sieve to obtain the infant rice flour.

Description

Technical Field

[0001] The invention relates to the technical field of infant food, and in particular to a method for preparing infant rice flour for reducing allergic symptoms in infants. Background Art

[0002] Infants and young children have a relatively high incidence of allergic reactions because their immune systems and body functions are not yet fully developed. In fact, a large number of studies have shown that infants and young children are significantly more likely to have allergies than people of other age groups. These allergic reactions may cause serious harm to their health, such as inability to eat, itchy skin, difficulty breathing, vomiting, diarrhea, etc., and in severe cases may even lead to shock or death. Inability to eat is a typical manifestation of allergic reactions in infants and young children. When children are allergic to certain foods or substances, they may experience symptoms such as swelling, pain and itching in the mouth or throat, causing the child to refuse to eat or have difficulty swallowing, thus affecting the child's nutritional intake and growth and development. In addition to being unable to eat, infant allergies may also affect the absorption of nutrients needed daily. Since children cannot eat normally, they may suffer from malnutrition, vitamin deficiency and other problems, which in turn affect the child's physical development and immunity. In order to ensure that children can get enough nutrients and grow up healthily, it is particularly important to identify allergens and take targeted treatment measures.

[0003] Allergic reactions play an important role in the maturation of infants' immune systems. This natural physiological reaction helps the body identify and reject foreign substances, thereby promoting the gradual maturation of the immune system. It is an important source of natural immunity for infants and is beneficial to normal immune development. The best way to deal with infant allergies is to take measures to reduce or alleviate allergic symptoms to ensure that children can successfully pass this sensitive stage.

[0004] Infant and young children's nutritional rice cereal is a supplementary food suitable for infants and young children over 6 months old, which is made of rice as the main raw material, with appropriate amounts of nutritional enhancers and (or) other auxiliary materials added. Rice accounts for about 95% of the raw materials and occupies a major position in the raw and auxiliary materials of infant and young children's nutritional rice cereal. Infant and young children's rice cereal is a food specially designed for infants and young children. The nutrients usually include protein, carbohydrates, fat, vitamins and minerals, which are essential for the growth and development of infants. Because this rice cereal is easy to digest, it can help infants better absorb these nutrients and promote their healthy growth. More importantly, infant and young children's rice cereal does not contain the eight major allergens, which means that it will hardly cause allergic reactions. This is undoubtedly a very good choice for those infants and young children who are prone to allergies. Therefore, compared with other foods, the probability of allergies in infant and young children's rice cereal is relatively low, and it is a good food source to reduce or relieve allergic symptoms.

[0005] However, in recent years, food allergies caused by rice have occurred frequently, and the incidence of allergies to infant nutritional rice cereal is still continuing to increase, which has had many adverse effects on the development and health of infants and young children. Therefore, food allergies in infants and young children have become a primary issue that needs to be urgently addressed.

[0006] Rice protein is the main source of protein in infant rice cereal, and rice and rice products must be processed before they can be eaten. At present, the processing methods used to change rice allergen proteins include bioprocessing, chemical processing, physical processing and other technologies. However, considering the safety and rationality of regulations, removing allergen components and using enzymatic hydrolysis have become the preferred options, but the protein in infant nutritional rice cereal mainly comes from rice protein in rice. Although removing some proteins in infant rice cereal can reduce its allergenicity, this practice will have an adverse effect on the nutritional intake of infants. Secondly, some of the latest studies have pointed out that the use of advanced enzymatic hydrolysis technology in infant rice cereal can significantly improve its digestibility and absorbability, and effectively reduce the intestinal discomfort that may occur after infants eat it. However, this technology has extremely high requirements in the implementation process, and the degree of enzymatic hydrolysis reaction needs to be precisely controlled, otherwise it may affect the quality of the final product. More importantly, new allergens may be generated or brought in during the enzymatic hydrolysis process, which may lead to an increase in allergenicity, so these technologies have certain limitations in application. Summary of the invention

[0007] In view of the current defects that protein needs to be removed to control the allergenicity of infant rice flour, which is not conducive to the nutritional needs of infants, and the high requirements for enzymatic hydrolysis technology, the need for accurate control of enzymatic hydrolysis technology, and the generation or introduction of new allergens, the first purpose of the present invention is to provide a method for preparing nutritious rice flour for infants, aiming to provide infant rice flour that is suitable for infants to eat, can meet the daily nutritional intake needs of infants, and also has low allergenicity.

[0008] The second purpose of the present invention is to provide infant nutritional rice flour prepared by the preparation method, which can reduce the allergenicity of the infant nutritional rice flour. At the same time, people who are allergic to milk can also relieve the symptoms of milk allergy by eating the infant rice flour, thereby achieving a soothing effect.

[0009] Different from conventional infant rice cereal, higher requirements are put forward for its nutritional properties and allergenicity control. However, in the existing preparation process of infant rice cereal, there may be problems such as removing rice protein is not conducive to the nutritional needs of infants and young children, and new allergens are introduced after enzymatic hydrolysis. In order to prepare exclusive infant rice cereal that can reduce and relieve the allergic symptoms of infants and young children, the problems of nutrient loss and increased allergenicity during processing can be solved.

[0010] To achieve the above object, the present invention provides the following technical solution: a method for preparing infant rice cereal for reducing allergic symptoms in infants, comprising the following steps:

[0011] Step 1: Sorting and cleaning of rice raw materials

[0012] Select GABA-rich sprouted rice for cleaning, remove sediment, impurities, bran layer, surface microorganisms and pesticide residues, take 94-96 parts of GABA-rich sprouted rice, 1-2 parts of mineral nutrient packages, 1-2 parts of vitamin nutrient packages, 1-2 parts of oligofructose, and 1-2 parts of coconut oil for later use;

[0013] Step 2: High static pressure pretreatment:

[0014] The cleaned GABA-rich germinated rice is put into a polyethylene plastic bag and completely sealed, and then placed in a pressure system of a high static pressure treatment device, and a high static pressure treatment is applied to the rice raw material to achieve the purpose of changing the physical and chemical properties of the rice, wherein the high static pressure treatment is performed at a pressure of 260 to 300 MPa, a temperature of 55 to 65° C., and a time of 10 to 15 minutes;

[0015] Step 3: High humidity steam quickly absorbs water:

[0016] The GABA-rich germinated rice treated with high static pressure is evenly spread on the wire mesh with a thickness of 1.8 to 2.2 cm, and a high-humidity steam rapid water absorption process is adopted to achieve the purpose of rapid water absorption and heating of the rice raw material. The GABA-rich germinated rice raw material is subjected to high-humidity steam for pretreatment, wherein the temperature of the high-humidity steam is 140 to 160° C., the humidity is 75% to 85%; the pretreatment time is 3 to 6 minutes; and the high-humidity steam flow rate is 2.5 to 4.0 m / s;

[0017] Step 4: Shearing and slurrying:

[0018] The GABA-rich rice raw material treated in step 3 is added to a pulping machine, the slurry obtained by slurrying is passed through a 100-mesh sieve, the retained matter in the sieve is less than 15%, the temperature is 50-55° C., and then 1-2 parts of a mineral nutrient package, 1-2 parts of a vitamin nutrient package, 1-2 parts of oligofructose, and 1-2 parts of coconut oil are added, mixed evenly, and temporarily stored to obtain rice slurry.

[0019] Step 5: Drum Drying:

[0020] The rice slurry in step 4 is fed into a preheated drum, and the rice slurry is evenly attached to the drum for aging, drying, sterilization, and enzyme inactivation, the air pressure of the drum is 0.5-0.60M Pa, the rotation speed is 1-5r / min, and the temperature is 120-130° C., and the rice slurry is dried into rice flakes;

[0021] Step 6: Finished product

[0022] The crushed and sieved infant rice flakes are crushed and then sieved through a 5mm sieve to obtain the infant rice cereal.

[0023] The invention uses germinated rice rich in GABA as a raw material, and utilizes a high static pressure combined with a high humidity steam treatment technology and a drum drying technology to produce infant rice flour with the function of reducing and relieving infant allergy symptoms. The prepared infant rice flour can not only meet the daily nutrient intake required by infants, but also provide a low-allergenic rice raw material, thereby reducing the allergenicity of the infant nutritious rice flour. Meanwhile, the germinated rice rich in GABA contains natural biological activity and soothing ingredients, and people who are allergic to milk can also relieve cow milk allergy symptoms by eating the infant rice flour, thereby achieving a soothing effect. This has important practical significance and social value for reducing and relieving the problem of allergies in infants after eating the infant rice flour.

[0024] High static pressure treatment can act on non-covalent bond structures in food, such as hydrophobic bonds, ionic bonds and hydrogen bonds, etc., and induce the unfolding of protein structure and expose the groups inside the protein by destroying these non-covalent bonds, destroying the spatial structure of allergens, and destroying the conformational epitopes of allergens, thereby achieving the effect of reducing or desensitizing; high humidity steam treatment technology refers to the technology of sterilizing, disinfecting, thawing, heating and keeping warm by using high temperature and high pressure steam, and can change the allergenicity of food. With high humidity steam treatment technology, food protein can form polymers under heating conditions, so that the antigenic epitopes of allergenic proteins in food are hidden inside and exposed, which is easy to cause changes in spatial structure. Changes in spatial structure can destroy the conformational epitopes of allergen proteins, thereby reducing the allergenicity of allergens. Compared with traditional heat treatment technology, high humidity steam treatment technology has the advantages of uniform temperature, fast heating speed, less loss of nutrients, good food taste, good sterilization effect, environmental protection and energy saving in food processing, which can improve production efficiency and product quality, while ensuring the hygiene, safety and nutritional value of food.

[0025] Secondly, sprouted rice refers to white rice that is naturally rich in γ-aminobutyric acid (GABA) > 200 mg / kg and retains 10% bran, germ, and endosperm. The bioactive substances contained in it mainly include GABA, a small amount of polyphenolic compounds, glutathione, fat-soluble antioxidants and minerals, etc. These substances have multiple biological functions, including anti-anxiety, lowering blood pressure, improving lipid metabolism, reducing neutral fat, anti-allergy and preventing arteriosclerosis. GABA is an amino acid with multiple biological activities. It can bind to and activate anti-anxiety brain receptors, fundamentally calming the nerves, thereby achieving an anti-anxiety effect. In addition, it can also lower blood pressure, improve lipid metabolism, reduce neutral fat, and prevent arteriosclerosis. In allergic reactions, GABA can inhibit the activity of immune cells, thereby alleviating allergic symptoms. Glutathione helps control various digestive system diseases, including irritable bowel syndrome, gastroenteritis, ulcerative colitis, chronic diseases and malabsorption syndrome. It can protect various organs of the digestive tract from inflammation, infection and cell damage, and prevent gastritis, ulcers and gastrointestinal cancer. In addition, glutathione is an important antioxidant that protects the body from damage by free radicals. In allergic reactions, glutathione can inhibit inflammatory reactions, reduce allergic symptoms, and regulate the function of the immune system to enhance the body's immunity. The main effects of polyphenols are antioxidant, anti-inflammatory, antibacterial and anti-allergic. Polyphenols have antioxidant effects, which can remove free radicals in the body and prevent aging and chronic diseases; they have anti-inflammatory effects, which can reduce inflammatory reactions, relieve pain and discomfort; they also have antibacterial effects, which can inhibit the growth and reproduction of pathogens and prevent infection. In terms of allergies, polyphenols can alleviate allergic symptoms by inhibiting the release of histamine. When the body is allergic to certain substances, histamine will be released to trigger allergic reactions. Polyphenols can inhibit the release of histamine, thereby alleviating allergic symptoms. Dietary fiber can promote intestinal health, lower blood sugar, lower cholesterol and prevent constipation. In addition, the dietary fiber in germinated rice has a certain effect in anti-allergy. Dietary fiber can promote intestinal health and increase the number of beneficial bacteria in the intestine, thereby improving the intestinal environment and reducing the occurrence of allergic reactions. Secondly, dietary fiber can reduce the permeability of the intestinal mucosa, reduce the penetration and absorption of allergens, and thus alleviate allergic symptoms.

[0026] In addition, dietary fiber can promote the excretion of toxins in the intestine, reduce the damage of toxins to the body, and help alleviate allergic symptoms. Pantothenic acid is a water-soluble vitamin in the B group, which has anti-allergic, anti-inflammatory and anti-infective effects. Pantothenic acid can inhibit the activity of immune cells, alleviate allergic symptoms, and promote the health of skin, hair and nails. Vitamin B6 is a water-soluble vitamin that has anti-allergic and anti-inflammatory effects. Vitamin B6 can inhibit the activity of immune cells, alleviate allergic symptoms, and promote protein metabolism and the health of the nervous system. Finally, after germination, the protein in rice becomes looser, making it easier to process it through physical technology to achieve the purpose of passivation. In this way, while fully retaining the nutritional value of the germinated rice itself, its allergenicity can be significantly reduced.

[0027] Beneficial effects of the present invention: The present invention innovatively uses germinated rice rich in GABA as the rice raw material, and further cooperates with the high static pressure pretreatment, high humidity steam pretreatment, shearing and pulping and drum drying treatment processes and the joint control of various process parameters, which can achieve synergy, can significantly reduce the problem of nutrient loss in the processing stage, and improve the problem of nutrient loss caused by reducing the allergenicity of infant rice flour. In addition, the infant rice flour preparation technology of the present invention can also reduce the allergenicity of infant nutritional rice flour, and avoid the generation of new allergens by enzymatic hydrolysis technology. Finally, the infant rice flour prepared by this method has the characteristic of soothing allergic reactions, helps to reduce and alleviate the allergic symptoms that occur after infants and young children consume milk, and plays a soothing role. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Attached Figure 1 This is a clinical symptom scoring diagram for Example 1 of the present invention and the comparison group.

[0029] Attached Figure 2 This is a statistical comparison chart of body temperature between Example 1 of the present invention and the control group.

[0030] Attached Figure 3 It is a statistical comparison chart of diarrhea rates between Example 1 of the present invention and the control group.

[0031] Attached Figure 4 This is a comparison chart of serum specific antibody IgG content between Example 1 of the present invention and the control group.

[0032] Attached Figure 5 This is a comparison chart of serum specific antibody IgE content between Example 1 of the present invention and the control group.

[0033] Attached Figure 6 This is a comparison chart of serum mMCP-1 content between rats in Example 1 of the present invention and a control group.

[0034] Attached Figure 7 The figure is a comparison chart of serum MCT content between rats in Example 1 of the present invention and the control group.

[0035] Attached Figure 8 The figure is a comparison chart of HIS content in the plasma of rats in Example 1 of the present invention and the control group.

[0036] Attached Fig. 9 This is a clinical symptom scoring diagram for Example 2 of the present invention and the comparison group.

[0037] Attached Fig.10 This is a statistical comparison chart of body temperature between Example 2 of the present invention and the control group.

[0038] Attached Fig.11 It is a statistical comparison chart of diarrhea rates between Example 2 of the present invention and the control group.

[0039] Attached Fig.12 This is a comparison chart of serum specific antibody IgG content between Example 2 of the present invention and the control group.

[0040] Attached Fig.13 This is a comparison chart of serum specific antibody IgE content between Example 2 of the present invention and the control group.

[0041] Attached Fig.14 This is a comparison chart of serum mMCP-1 content between rats in Example 2 of the present invention and the control group.

[0042] Attached Fig.15 The figure is a comparison chart of serum MCT content between rats in Example 2 of the present invention and the control group.

[0043] Attached Fig.16 The figure is a comparison chart of HIS content in the plasma of rats in Example 2 of the present invention and the control group. DETAILED DESCRIPTION

[0044] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0045] Implementation 1

[0046] In this embodiment 1, the finished product of embodiment 1 and 7 finished products of comparative examples were prepared according to different recipes and compared through experiments.

[0047] Example 1 Finished product preparation:

[0048] The present embodiment provides an infant rice cereal for reducing and relieving allergic symptoms in infants and young children, which is prepared from the following raw materials in parts by weight: 96 parts of GABA-rich germinated rice, 1.5 parts of a mineral nutrient package, 1.5 parts of a vitamin nutrient package, 0.5 parts of oligofructose, and 0.5 parts of coconut oil;

[0049] The specific steps are as follows:

[0050] 1. Sorting and cleaning of rice raw materials: GABA-rich germinated rice is used as the rice raw material (96 portions of GABA-rich germinated rice). The rice is poured into a rice hopper, which is placed on a water saver. The water saver is filled with water. During the cleaning process, gravel enters the gravel hopper, and floating objects are filtered when they pass through the floating object screen with the water flow, so as to clean and remove the mud impurities, bran layer, surface microorganisms and pesticide residues on the surface of the rice; the working water pressure of the rice cleaning machine is 0.23-0.28MPa, and the stone discharge pressure is 0.8-1.0MPa.

[0051] 2. High static pressure treatment: Put the cleaned rice raw material (96 parts of GABA-rich germinated rice) into a polyethylene plastic bag and completely seal it, put it into the pressure system of the high static pressure treatment equipment, and apply high static pressure treatment to the rice raw material to achieve the purpose of changing the physical and chemical properties of the rice. Among them, in the high static pressure treatment, the pressure is 260-300Mpa, the temperature is 55-65℃, and the time is 10-15min;

[0052] 3. Rapid water absorption by high-humidity steam: The rice raw material (96 parts of GABA-rich germinated rice) after high static pressure treatment is evenly spread on the wire mesh with a thickness of 1.8 to 2.2 cm. A rapid water absorption process of high-humidity steam is adopted to achieve the purpose of rapid water absorption and heating of the rice raw material. High-humidity steam is applied to the GABA-rich germinated rice raw material for pretreatment, wherein the temperature of the high-humidity steam is 140 to 160° C., the humidity is 75% to 85%; the pretreatment time is 5 min; the high-humidity steam flow rate is 3 m / s;

[0053] 4. Shearing and slurrying: add the rice raw material after the treatment in step 3 into a pulping machine, slurry obtained by slurrying is passed through a 100 mesh sieve, the interception in the sieve is less than 15%, the temperature is 50-55° C., then add 1.5 parts of mineral nutrient packages, 1.5 parts of vitamin nutrient packages, 0.5 parts of oligofructose, and 0.5 parts of coconut oil, mix well, and obtain rice slurry after temporary storage treatment.

[0054] 5. Drum drying: the rice slurry in step (4) is fed into a preheated drum, and the rice slurry is evenly attached to the drum for slaking, drying, sterilization and enzyme inactivation. The air pressure of the drum is 0.5-0.60 MPa, the rotating speed is 1-5 r / min, and the temperature is 120-130° C. to dry the rice slurry into rice flakes.

[0055] 6. The crushed and sieved infant rice flakes are crushed and then sieved through a 5 mm sieve to obtain the infant rice cereal.

[0056] Comparative Example 1:

[0057] Compared with Example 1, the only difference is that the number of GABA-rich sprouted rice and Northeastern pearl rice in the rice raw material is: 64 parts of GABA-rich sprouted rice and 32 parts of Northeastern pearl rice. The specific production process is the same as that of Example 1.

[0058] Comparative Example 2:

[0059] Compared with Example 1, the only difference is that the number of GABA-rich sprouted rice and Northeastern pearl rice in the rice raw material is: 32 parts of GABA-rich sprouted rice and 64 parts of Northeastern pearl rice. The specific production process is the same as that of Example 1.

[0060] Comparative Example 3:

[0061] Compared with Example 1, the only difference is that the number of GABA-rich sprouted rice and Northeastern pearl rice in the rice raw material is: 0 part of GABA-rich sprouted rice and 96 parts of Northeastern pearl rice. The specific production process is the same as that of Example 1.

[0062] Comparative Example 4:

[0063] Compared with Example 1, the only difference is that the number of GABA-rich sprouted rice and northeastern pearl rice in the rice raw material is: 96 parts of GABA-rich sprouted rice and 0 parts of northeastern pearl rice. The specific production process is different from Example 1 in that the process does not have the treatment of steps (2) and (3), that is, the rice raw material is treated by Example 1, and then soaked, the soaking temperature is 20-25° C., the time is 45-55 minutes, and the remaining steps after soaking are the same as (4) to (6) in Example 1.

[0064] Comparative Example 5:

[0065] Compared with Comparative Example 4, the only difference is that the number of GABA-rich sprouted rice and Northeastern pearl rice in the rice raw material is: 64 parts of GABA-rich sprouted rice and 32 parts of Northeastern pearl rice. The specific production process is the same as that of Comparative Example 4.

[0066] Comparative Example 6:

[0067] Compared with Comparative Example 4, the only difference is that the number of GABA-rich sprouted rice and Northeastern pearl rice in the rice raw materials is: 32 parts of GABA-rich sprouted rice and 64 parts of Northeastern pearl rice. The specific production process is the same as that of Comparative Example 4.

[0068] Comparative Example 7:

[0069] Compared with Comparative Example 4, the only difference is that the number of GABA-rich sprouted rice and Northeastern pearl rice in the rice raw material is: 0 part of GABA-rich sprouted rice and 96 parts of Northeastern pearl rice. The specific production process is the same as that of Comparative Example 4.

[0070] The rice cereals for infants prepared in Example 1 and Comparative Examples 1 to 7 were subjected to animal experimental control studies, and the specific methods are as follows:

[0071] A rat infant rice cereal allergy model was established using weaned Wistar rats, 3 weeks old, weighing 45-65 g. The clinical symptoms, body temperature, diarrhea, and biochemical indices of the rats were measured. The results showed that the infant nutritional rice cereal of Example 1 had the lowest allergenicity and was not significantly different from the blank group.

[0072] 1) Clinical symptom score

[0073] The test scored the clinical symptoms of Wistar rats after allergy stimulation. Figure 1 shown.

[0074] Depend on Figure 1 It can be seen that, except for the blank group, the rats in Example 1 and Comparative Examples 1-7 groups all showed different degrees of clinical symptom reactions. Among them, the rats in the blank group showed no signs of allergic symptoms; compared with the blank group, some rats in Comparative Example 7 group showed severe allergic symptoms, and one rat showed severe allergic phenomenon of death due to shock; compared with Example 1 group, all rats in Comparative Examples 1 to 7 groups showed higher allergic symptom reactions than those in Example 1 group, and only one rat in Example 1 group showed mild allergic reaction, with low clinical symptom scores, and no significant difference with the blank group (P>0.05). Compared with the blank group and Example 1, there were still mild allergic symptom reactions in Comparative Examples 1-7 groups. Compared with Comparative Examples 1-3 groups, the clinical symptom scores of Example 1 group increased successively; compared with Comparative Examples 4 and Comparative Examples 5-7 groups, the clinical symptom scores also increased successively. Therefore, the higher the amount of GABA-rich germinated rice added, the more significant the reduction of allergic reaction symptoms. The comparison results between Example 1 and Comparative Example 4, Comparative Example 1 and Comparative Example 5, Comparative Example 2 and Comparative Example 6, and Comparative Example 3 and Comparative Example 7 show that the use of high static pressure and high humidity steam combined treatment technology can significantly reduce the symptoms of allergic reactions.

[0075] In summary, the use of GABA-rich germinated rice combined with high static pressure and high humidity steam treatment technology can significantly reduce infant rice cereal allergic reactions. Therefore, in terms of reducing allergic reaction symptoms, Example 1 has a more significant effect than other groups.

[0076] 2) Rat body temperature index

[0077] The test was conducted to statistically analyze the body temperature of Wistar rats after allergic stimulation. The results are as follows: Figure 2 shown.

[0078] Studies have shown that when rats show clinical allergic symptoms, their body temperature drops rapidly and their activity is greatly reduced. Figure 2 It can be seen that the body temperature of the rats in the blank group was normal, with an average body temperature of 37.61°C; compared with the blank group, the body temperatures of Example 1 and Comparative Examples 1-7 groups were slightly lower, but there was no significant difference (P>0.05); in addition, the body temperatures of the rats in Comparative Example 2, Comparative Example 4 and Comparative Example 6 groups rebounded to 37.66°C, 37.68°C and 37.68°C, respectively, which were slightly higher than those of Example 1 group, but there was no significant difference compared with the blank group (P>0.05).

[0079] Compared with the comparative examples 1 and 3, the body temperature of the group 1 of Example 1 was successively reduced; compared with the comparative example 5 and the comparative example 7, the body temperature was also successively reduced. Therefore, the body temperature of the animals will decrease with the increase of the amount of GABA-rich germinated rice added. The results of the mutual comparison between Example 1 and Comparative Example 4, Comparative Example 1 and Comparative Example 5, Comparative Example 2 and Comparative Example 6, and Comparative Example 3 and Comparative Example 7 show that the body temperature of the animals shows a slight downward trend by using the high static pressure and high humidity steam combined treatment technology.

[0080] In summary, the combination of high static pressure, high humidity steam synergistic treatment technology and increasing the proportion of GABA-rich germinated rice can effectively reduce and alleviate clinical allergic reaction symptoms. Therefore, in terms of reducing allergic reaction symptoms, Example 1 has a positive effect compared with other groups.

[0081] 3) Diarrhea in rats

[0082] The experiment counted the diarrhea rate of Wistar rats after allergic stimulation, and the results were as follows: Figure 3 shown.

[0083] like Figure 3 As shown, the blank group did not show diarrhea symptoms, and only one rat in the Example 1 group showed mild diarrhea reaction, which was not significantly different from the blank group (P>0.05); compared with the blank group and Example 1, the rats in the Comparative Examples 1-7 groups showed diarrhea, and the incidence of diarrhea showed an increasing trend, and the diarrhea in Comparative Example 7 was the most obvious; compared with the Example 1 group, the diarrhea rates of Comparative Example 1, Comparative Example 2 and Comparative Example 3 were 12.5%, 15.0% and 25.0% respectively; compared with the Comparative Example 4 group, the diarrhea rates of Comparative Example 5, Comparative Example 6 and Comparative Example 7 were 37.5%, 37.5% and 50% respectively; therefore, the diarrhea rate of animals will increase with the decrease in the amount of GABA-rich germinated rice added. The results of the mutual comparison between Example 1 and Comparative Example 4, Comparative Example 1 and Comparative Example 5, Comparative Example 2 and Comparative Example 6, and Comparative Example 3 and Comparative Example 7 show that the diarrhea rate of animals is decreasing by using the high static pressure and high humidity steam combined treatment technology.

[0084] In summary, the combination of high static pressure, high humidity steam synergistic treatment technology and increasing the proportion of GABA-rich germinated rice added can most effectively and significantly reduce the allergic reaction symptoms of infant rice cereal. Therefore, in terms of reducing allergic reaction symptoms, Example 1 has a more significant effect than other groups.

[0085] 4) Analysis of rat serum specific antibodies IgG and IgE

[0086] The test analyzed the specific antibodies IgG and IgE in rat serum. Figure 4-5 shown.

[0087] The experiment analyzed the serum IgG and IgE of Wistar rats. The results are as follows Figure 4-5 As shown. There was no significant difference in serum IgG and IgE between the blank group and Example 1 (P>0.05). Compared with the blank group and Example 1, the IgE and IgG contents of Comparative Examples 1-7 increased. Compared with Comparative Examples 1-3, the IgE and IgG contents of Example 1 increased successively; compared with Comparative Example 4 and Comparative Examples 5-7, the IgE and IgG contents also increased successively; therefore, the IgE and IgG contents will increase with the decrease in the amount of GABA-rich germinated rice added. The comparison results of Example 1 with Comparative Example 4, Comparative Example 1 with Comparative Example 5, Comparative Example 2 with Comparative Example 6, and Comparative Example 3 with Comparative Example 7 show that the IgE and IgG contents tend to decrease when the high static pressure and high humidity steam combined treatment technology is adopted.

[0088] In summary, the combination of high static pressure, high humidity steam synergistic treatment technology and increasing the proportion of GABA-rich germinated rice addition can most effectively and significantly reduce IgE and IgG content and reduce allergic reaction symptoms. Therefore, in terms of reducing allergic reaction symptoms, Example 1 has a more significant effect than other groups.

[0089] 5) Analysis of rat serum mast cell protease (mMCP-1)

[0090] The experiment analyzed rat serum mMCP-1, and the results were as follows Figure 6 shown.

[0091] Depend on Figure 6It can be seen that there is no significant difference in serum mMCP-1 between the blank group and Example 1 (P>0.05). Compared with the blank group and Example 1, the mMCP-1 content of Comparative Examples 1-7 increased. Compared with Comparative Examples 1-3, the mMCP-1 content of Example 1 increased successively; compared with Comparative Example 4 and Comparative Examples 5-7, the mMCP-1 content also increased successively; therefore, the mMCP-1 content will increase with the decrease in the amount of GABA-rich germinated rice added. The results of mutual comparison between Example 1 and Comparative Example 4, Comparative Example 1 and Comparative Example 5, Comparative Example 2 and Comparative Example 6, and Comparative Example 3 and Comparative Example 7 show that the mMCP-1 content tends to decrease when the high static pressure and high humidity steam combined treatment technology is adopted.

[0092] In summary, the combination of high static pressure, high humidity steam synergistic treatment technology and increasing the proportion of GABA-rich germinated rice can most effectively and significantly reduce the mMCP-1 content and reduce allergic reaction symptoms. Therefore, in terms of reducing allergic reaction symptoms, Example 1 has a more significant effect than other groups.

[0093] 6) Analysis of rat serum tryptase (MCT)

[0094] The experiment analyzed the serum MCT of Wistar rats. The results are as follows Figure 7 shown.

[0095] Depend on Figure 7 It can be seen that there is no significant difference in serum MCT between the blank group and Example 1 (P>0.05). Compared with the blank group and Example 1, the MCT content of Comparative Examples 1-7 increased. Compared with Comparative Examples 1-3, the MCT content of Example 1 increased successively; compared with Comparative Example 4 and Comparative Examples 5-7, the MCT content also increased successively; therefore, the MCT content will increase with the decrease in the amount of GABA-rich germinated rice added. The results of mutual comparison between Example 1 and Comparative Example 4, Comparative Example 1 and Comparative Example 5, Comparative Example 2 and Comparative Example 6, and Comparative Example 3 and Comparative Example 7 show that the MCT content tends to decrease by using the high static pressure and high humidity steam combined treatment technology.

[0096] In summary, the combination of high static pressure, high humidity steam synergistic treatment technology and increasing the proportion of GABA-rich sprouted rice can most effectively and significantly reduce the MCT content and reduce allergic reaction symptoms. Therefore, in terms of reducing allergic reaction symptoms, Example 1 has a more significant effect than other groups.

[0097] 7) Analysis of histamine (HIS) in rat plasma

[0098] The experiment analyzed the histamine in the plasma of Wistar rats. The results are as follows Figure 8 shown.

[0099] Depend on Figure 8 It can be seen that there is no significant difference in HIS between the blank group and Example 1 (P>0.05). Compared with the blank group and Example 1, the HIS content of Comparative Examples 1-7 groups increases successively. Compared with Comparative Examples 1-3, the HIS content of Example 1 group increases successively; compared with Comparative Example 4 and Comparative Examples 5-7, the HIS content also increases successively; therefore, the HIS content will increase with the decrease in the amount of GABA-rich germinated rice added. The comparison results of Example 1 and Comparative Example 4, Comparative Example 1 and Comparative Example 5, Comparative Example 2 and Comparative Example 6, and Comparative Example 3 and Comparative Example 7 show that the HIS content tends to decrease when the high static pressure and high humidity steam combined treatment technology is used.

[0100] In summary, the combination of high static pressure, high humidity steam synergistic treatment technology and increasing the proportion of GABA-rich germinated rice can most effectively and significantly reduce HIS content and reduce allergic reaction symptoms. Therefore, in terms of reducing allergic reaction symptoms, Example 1 has a more significant effect than other groups.

[0101] Example 2

[0102] According to the results of Experimental Example 1, the infant rice cereal prepared in Example 1 was selected for animal experimental control research, and the specific method was as follows:

[0103] Weaned Wistar rats, 3 weeks old, weighing 45-65 g, were used as the subjects and a rat milk allergy model was adopted. By measuring the clinical symptoms, body temperature, diarrhea, and biochemical indicators of the rats, it was found that the infant nutritional rice cereal of Example 1 can alleviate the symptoms of milk allergy and achieve a soothing effect.

[0104] 1) Clinical symptom score

[0105] The experiment characterized the clinical symptoms of Wistar rats. Fig. 9 shown.

[0106] Depend on Fig. 9 It can be seen that, except for the blank group, the rats in the control group and Example 1 all showed clinical symptom reactions to varying degrees. Among them, the rats in the blank group showed no signs of allergic symptoms; compared with the control group and Example 1, some rats in the control group showed severe allergic symptoms, and 2 rats showed severe allergic phenomena and died of shock. Compared with the Example 1 group, the allergic symptom reactions of all rats in the control group were higher than those in the Example 1 group. In addition, only mild allergic reactions occurred in the Example 1 group, and the clinical symptom scores were significantly reduced. Therefore, the rice noodles in Example 1 prepared by using germinated rice rich in GABA and high static pressure and high humidity steam treatment technology can relieve the symptoms of cow's milk allergy and achieve a soothing effect.

[0107] In summary, the use of GABA-rich germinated rice and high static pressure, high humidity steam treatment technology can significantly inhibit allergic reactions caused by milk intake, relieve milk allergy symptoms, and achieve a soothing effect.

[0108] 2) Rat body temperature index

[0109] The experiment conducted statistics on the body temperature of Wistar rats, and the results are as follows Fig.10 shown.

[0110] Studies have shown that when rats show clinical allergic symptoms, their body temperature drops rapidly and their activity is greatly reduced. Fig.10 It can be seen that the body temperature of the rats in the blank group was normal, with an average body temperature of 37.69°C; compared with the blank group, the body temperatures of the control group and Example 1 group were slightly elevated, but there was no significant difference. Compared with Example 1, the average body temperature of the control group was 36.85°C, and the average body temperature of Example 1 was 37.45°C, so the body temperature of the control group was higher than that of Example 1 group, and the body temperature of the animals was elevated. Therefore, the rice flour of Example 1 prepared by using germinated rice rich in GABA and high static pressure and high humidity steam treatment technology can relieve the symptoms of cow milk allergy and achieve a soothing effect.

[0111] In summary, the use of GABA-rich germinated rice and high static pressure, high humidity steam treatment technology can significantly inhibit allergic reactions caused by milk intake, relieve milk allergy symptoms, and achieve a soothing effect.

[0112] 3) Diarrhea in rats

[0113] The test was conducted to statistically analyze the diarrhea rate of Wistar rats with allergies. The results are as follows: Fig.11 shown.

[0114] like Fig.11 As shown, the rats in the blank group did not have diarrhea symptoms; compared with the blank group, the rats in the control group and Example 1 had diarrhea, and the incidence of diarrhea showed a decreasing trend, and the diarrhea in the control group was more obvious; compared with the control group and Example 1 group, the diarrhea rates were 50.0% and 25.0%, respectively, and the diarrhea rate of animals after taking Example 1 showed a decreasing trend. Therefore, the rice flour of Example 1 prepared by using germinated rice rich in GABA and high static pressure and high humidity steam treatment technology can relieve the symptoms of cow milk allergy and achieve a soothing effect.

[0115] In summary, the use of GABA-rich germinated rice and high static pressure, high humidity steam treatment technology can significantly inhibit allergic reactions caused by milk intake, relieve milk allergy symptoms, and achieve a soothing effect.

[0116] 4) Analysis of rat serum specific antibodies IgG and IgE

[0117] The test was conducted on the serum specific IgG and IgE of Wistar rats. The results are as follows Figure 12-13 shown.

[0118] The experiment analyzed the serum IgG and IgE of Wistar rats. The results are as follows Figure 12-13 As shown. Compared with the blank group, the IgG and IgE contents of the control group and the Example 1 group were increased. Compared with the Example 1 group, the IgG and IgE contents of the control group were significantly reduced. Therefore, the rice flour of Example 1 prepared by using germinated rice rich in GABA and high static pressure and high humidity steam treatment technology can alleviate the symptoms of cow milk allergy and achieve a soothing effect.

[0119] In summary, the use of GABA-rich germinated rice and high static pressure, high humidity steam treatment technology can significantly inhibit allergic reactions caused by milk intake, relieve milk allergy symptoms, and achieve a soothing effect.

[0120] 5) Analysis of rat serum mast cell protease (mMCP-1)

[0121] The experiment analyzed rat serum mMCP-1, and the results were as follows Fig.14 shown.

[0122] The experiment analyzed the serum mMCP-1 of Wistar rats. The results are as follows Fig.14 As shown. Compared with the blank group, the mMCP-1 content of the control group and the Example 1 group increased. Compared with the Example 1 group, the mMCP-1 content of the control group was significantly reduced. Therefore, the rice flour of Example 1 prepared by using germinated rice rich in GABA and high static pressure and high humidity steam treatment technology can relieve the symptoms of cow milk allergy and achieve a soothing effect.

[0123] In summary, the use of GABA-rich germinated rice and high static pressure, high humidity steam treatment technology can significantly inhibit allergic reactions caused by milk intake, relieve milk allergy symptoms, and achieve a soothing effect.

[0124] 6) Analysis of rat serum tryptase (MCT)

[0125] The experiment analyzed the serum MCT of Wistar rats. The results are as follows Fig.15 shown.

[0126] The experiment analyzed the serum MCT of Wistar rats. The results are as follows Figure 7 As shown. Compared with the blank group, the contents of the control group and the Example 1 group were increased. Compared with the Example 1 group, the MCT content of the control group was significantly reduced. Therefore, the rice flour of Example 1 prepared by using germinated rice rich in GABA and high static pressure and high humidity steam treatment technology can relieve the symptoms of cow milk allergy and achieve a soothing effect.

[0127] In summary, the use of GABA-rich germinated rice and high static pressure, high humidity steam treatment technology can significantly inhibit allergic reactions caused by milk intake, relieve milk allergy symptoms, and achieve a soothing effect.

[0128] 7) Histamine analysis in rat plasma (HIS)

[0129] The experiment analyzed HIS in the plasma of Wistar rats, and the results were as follows Fig.16 shown.

[0130] The experiment analyzed the serum HIS of Wistar rats, and the results were as follows Fig.16 As shown. Compared with the blank group, the contents of the control group and the Example 1 group were increased. Compared with the control group and the Example 1 group, the HIS content decreased successively. Therefore, the rice flour of Example 1 prepared by using germinated rice rich in GABA and high static pressure and high humidity steam treatment technology can alleviate the symptoms of cow milk allergy and achieve a soothing effect.

[0131] In summary, the use of GABA-rich germinated rice and high static pressure, high humidity steam treatment technology can significantly inhibit allergic reactions caused by milk intake, relieve milk allergy symptoms, and achieve a soothing effect.

[0132] Conclusion: According to the results of Experimental Examples 1 and 2, the use of GABA-rich germinated rice and high static pressure, high humidity steam treatment technology can significantly inhibit infant rice cereal allergic reactions and reduce the allergenicity of infant rice cereal. At the same time, combining high static pressure, high humidity steam synergistic treatment technology with increasing the proportion of GABA-rich germinated rice addition can most effectively and significantly reduce and alleviate the symptoms of cow's milk allergy reactions and play a soothing role.

[0133] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for preparing rice cereal for infants and young children for reducing allergic symptoms in infants and young children, characterized in that: It includes the following steps: Step 1: sorting and cleaning of rice raw materials: select GABA-rich sprouted rice for cleaning, remove sediment, impurities, bran layer, surface microorganisms and pesticide residues, take 94-96 parts of GABA-rich sprouted rice, 1-2 parts of mineral nutrient packages, 1-2 parts of vitamin nutrient packages, 1-2 parts of oligofructose, and 1-2 parts of coconut oil for later use; Step 2: High static pressure pretreatment: The cleaned GABA-rich germinated rice is put into a polyethylene plastic bag and completely sealed, and placed in a pressure system of a high static pressure treatment device, and a high static pressure treatment is applied to the rice raw material to achieve the purpose of changing the physical and chemical properties of the rice; Step 3: High humidity steam rapid water absorption treatment: The GABA-rich germinated rice treated with high static pressure is evenly spread on the wire mesh with a thickness of 1.8 to 2.2 cm, and a high-humidity steam rapid water absorption process is adopted to achieve the purpose of rapid water absorption and heating of the rice raw material, and the GABA-rich germinated rice raw material is subjected to high-humidity steam for pretreatment; Step 4: Shearing and slurrying: Add the GABA-rich rice raw material treated in step 3 into a pulping machine, pass the pulping slurry through a 100-mesh sieve, the retained matter in the sieve is less than 15%, the temperature is 50-55° C., then add 1-2 parts of a mineral nutrient package, 1-2 parts of a vitamin nutrient package, 1-2 parts of oligofructose, and 1-2 parts of coconut oil, mix well, and obtain rice slurry after temporary storage treatment; Step 5: Drum Drying: The rice slurry in step 4 is fed into a preheated drum, and the rice slurry is evenly attached to the drum for aging, drying, sterilization, and enzyme inactivation, the air pressure of the drum is 0.5-0.60M Pa, the rotation speed is 1-5r / min, and the temperature is 120-130° C., and the rice slurry is dried into rice flakes; Step 6: Finished product: The crushed and sieved infant rice flakes are crushed and then sieved through a 5mm sieve to obtain the infant rice cereal.

2. The method for preparing rice cereal for infants and young children for reducing allergic symptoms in infants and young children according to claim 1, characterized in that: In step 2, the high static pressure treatment is performed at a pressure of 260 to 300 MPa, a temperature of 55 to 65° C., and a time of 10 to 15 min.

3. The method for preparing rice cereal for infants and young children for reducing allergic symptoms in infants and young children according to claim 1, characterized in that: In step 3, the temperature of the high-humidity steam is 140-160° C., and the humidity is 75%-85%; the pretreatment time is 3-6 minutes; and the flow rate of the high-humidity steam is 2.5-4.0 m / s.

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