Biosynthetic Chinese herbal medicine anti-hair loss pilatory and preparation method thereof

Abstract

The invention belongs to the technical field of biosynthesis and traditional Chinese medicine essence extraction, and particularly relates to a biosynthesis Chinese herbal medicine anti-hair loss pilatory and a preparation method thereof. The biosynthetic Chinese herbal medicine hair loss preventing and hair growing agent comprises the following effective components: a pseudomonas subtilis fermentation extract and a saccharomycetes fermentation extract. The hair-loss-preventing and hair-growing shampoo has the effects of preventing hair loss and growing hair through complementation and synergistic interaction of cleaning pores, inhibiting fungal reproduction, penetrating nutrient components into scalp and the like, activating dermis hair follicles, expanding blood capillaries, removing slough and promoting tissue regeneration, and promoting hair cell division, and has a relatively good application prospect.

Description

Technical Field

[0001] The invention belongs to the technical field of biosynthesis and extraction of Chinese herbal essence, and in particular relates to a biosynthetic Chinese herbal anti-hair loss and hair growth agent and a preparation method thereof. Background Art

[0002] The proportion of people suffering from hair loss is indeed very high. Data shows that the number of people suffering from hair loss in my country has exceeded 250 million (about 163 million men and about 88 million women, which is equivalent to 1 in 4 men and 1 in 8 women suffering from hair loss. At the same time, the proportion of hair loss before the age of 30 is as high as 84%, which is 20 years earlier than the age of hair loss in the previous generation, showing a clear trend of younger age. Therefore, anti-hair loss and hair growth agents have become a major demand.

[0003] Hair loss refers to the phenomenon of hair loss. Normal hair loss is in the catagen and telogen phases. Since the hair entering the catagen and the hair entering the new growth phase are constantly in a dynamic balance, the normal amount of hair can be maintained. Pathological hair loss refers to abnormal or excessive hair loss. There are many reasons, including hormonal, neurological, nutritional, endocrine, physical, infectious, chemical, etc. Among them, most people currently use shampoos made from petroleum hydrocarbon chemical synthetic raw materials. Long-term use will cause frizzy, dry, split hair, and scalp cracking. At the same time, these chemically synthesized components cause pore pollution, blockage, hair follicle cell damage, etc. due to strong adsorption, which aggravates the phenomenon of hair loss. There are three common types of hair loss: androgenic alopecia, alopecia areata caused by various reasons such as stress, and hair loss caused by drugs or diseases. The most common one is androgenic alopecia, which is characterized by progressive hair loss caused by the miniaturization of hair follicles due to male hormone stimulation, and the incidence rate gradually increases with age. However, although AGA is often referred to as "male hair loss", it can affect not only men but also women. AGA causes the hairline on the forehead of men to move back, the hair on the sides gradually becomes sparse, and the hair on the top becomes thinner and thinner. The hair loss is "horseshoe-shaped". As it progresses, it may develop to the point where only the back of the head and around the temples on both sides have remaining hair.

[0004] For women, androgenic alopecia is generally not as serious as baldness, and the impact on the forehead hairline is not obvious. Most women have sparse hair on the top of their heads, and a "Christmas tree"-shaped hair loss occurs. In addition, the scalp also needs a balance of water and oil. Once the balance of water and oil is broken, the sebaceous glands will secrete more oil to inhibit excessive water loss from the skin mucosa. Therefore, over-degreasing shampoo will make the hair oilier and oilier, not only increasing dandruff, but also causing itchy scalp, tight and dry scalp, affecting hair quality and causing further hair loss.

[0005] In recent years, with the improvement of people's living standards, the pollution of the living environment has become more and more serious, the psychological pressure has increased, and people pay more attention to their own image. Hair loss has become a top priority for people, bringing great mental pressure and pain to people. At present, most of the anti-hair loss and hair growth products on the market have the problem of ineffectiveness or large side effects. The current clinical treatment of androgenic alopecia mainly uses topical minoxidil and oral finasteride, but many patients actually find it difficult to stick to it. There are two reasons for this. First of all, topical minoxidil needs to be used every day, and many people are busy at work and it is difficult to stick to it. In addition, the use of minoxidil causes side effects, such as increased oil secretion of hair, sensitive inflammation of the scalp, and acne. Especially in the early stage of minoxidil use, there will be a period of crazy hair loss because of the activation of the physiological cycle of hair follicles. Regarding finasteride, its working principle is to inhibit the activity of 5α-reductase, so it is effective for androgenic alopecia, but changing hormone levels is also a double-edged sword. It is difficult to be limited to the part of hair loss, and it may also have a negative impact on other organs of the body, such as sexual function, or breast pain and other problems.

[0006] To this end, it is necessary to design a natural or fully biosynthetic compound that can overcome the problems of existing technologies that cannot take into account green environmental protection, health, non-irritation, and no side effects. Summary of the invention

[0007] In view of the problems existing in existing products, the purpose of the present invention is to solve the problems that most of the existing anti-hair loss products and technologies on the market have ineffective effects or serious side effects. The present invention provides a biosynthetic anti-hair loss and hair growth agent and a preparation method thereof, and uses biosynthesis technology to extract the anti-hair loss and hair growth essence from natural Chinese herbal medicines. To achieve the above object, the technical solution adopted by the present invention is: a biosynthetic Chinese herbal anti-hair loss and hair growth agent, the biosynthetic Chinese herbal anti-hair loss and hair growth agent comprising the following effective components: Pseudomonas subtilis fermentation extract and yeast fermentation extract.

[0008] Furthermore, the fermentation raw materials of the Pseudomonas subtilis fermentation extract include any one or more of the Chinese herbal medicines of wolfberry, angelica, Panax notoginseng, glutinous rice, epimedium, Gleditsia sinensis, ginseng, black sesame, and Polygonum multiflorum.

[0009] Furthermore, the fermentation raw materials of the yeast fermentation extract include any one or more of the Chinese herbal medicines selected from wolfberry, angelica, Panax notoginseng, glutinous rice, epimedium, Gleditsia sinensis, ginseng, black sesame and Polygonum multiflorum.

[0010] Furthermore, the mass fraction of the Pseudomonas fermentation extract is 10-90%, and the mass fraction of the yeast fermentation extract is 10-90%.

[0011] Furthermore, the microorganisms used in the Pseudomonas subtilis fermentation extract include any one or a mixture of Pseudomonas and Burkholderia and Bacillus.

[0012] Furthermore, the microorganisms used in the yeast fermentation extract include any one or more of Candida, Saccharomyces cerevisiae, and Pseudozyma.

[0013] Specific bacteria and yeasts are used to release anti-hair loss and hair growth molecules through the decomposition of the herbs; at the same time, other compounds that are beneficial or promote the enhanced efficacy are synthesized during the fermentation process.

[0014] Based on the above inventive concept, the present invention also provides a method for preparing a biosynthetic Chinese herbal anti-hair loss and hair growth agent, the preparation method specifically comprising the following steps: S1. Using Chinese medicinal herbs such as wolfberry, angelica, notoginseng, glutinous rice, epimedium, honeysuckle, ginseng, black sesame and polygonum multiflorum as the main carbon source, grinding them into powder according to proportion, mixing with grape seed oil, adding nitrate and phosphate inorganic culture components, and fermenting with microorganisms used for Pseudomonas fermentation extract at 20-40°C for 7-10 days; S2, using Chinese herbal medicines such as wolfberry, angelica, notoginseng, glutinous rice, epimedium, honey locust thorn, ginseng, black sesame and polygonum multiflorum as the main carbon source, grinding them into powder according to proportion, mixing with fructose, rhamnose, mannose and erythritol, adding nitrate and phosphate inorganic culture components, and fermenting with microorganisms used for yeast fermentation extract at 20-40°C for 10-14 days; S3, mixing the fermentation liquid obtained from S1 and S2 according to the proportion, and continuing to ferment at 25-35° C. for 3-7 days; S4. The mixed fermentation liquid obtained in step S3 is cultured for one day by introducing CO2 and stirred at high temperature for 8 hours, and then the pH is adjusted to be greater than 7, and then centrifuged, filtered and concentrated to obtain a hair growth agent.

[0015] Furthermore, the mixing ratio of the fermentation broth obtained by S1 and S2 in step S3 is 1:9-9:1 by mass.

[0016] Compared with the prior art, the present invention has the following advantages: 1. The biosynthesized anti-hair loss and hair growth agent of the present invention is a biological anti-hair loss and hair growth agent fermented by microorganisms rather than a traditional chemically synthesized biological anti-hair loss and hair growth agent. It cleans pores, inhibits fungal reproduction, and allows nutrient components to penetrate into the scalp, synergistically enhance synergy, activate dermal hair follicles, expand capillaries, remove dead tissue and promote new growth, and promote the division of hair matrix cells, thereby achieving the effect of hair growth.

[0017] 2. The biosynthesized anti-hair loss and hair growth agent of the present invention contains effective natural ingredients such as a variety of Chinese herbal medicine extracts and amphoteric active molecules, is non-hormonal, and is harmless to humans, animals, and the ecological environment.

[0018] 3. The preparation method of the present invention is simple, the conditions are mild, the production cost is low, the quality of the prepared anti-hair loss and hair growth agent is stable, and it has good market application prospects.

[0019] It should be appreciated that all combinations of the foregoing concepts, as well as additional concepts described in greater detail below, may be considered to be part of the inventive subject matter of the present disclosure, provided such concepts are not mutually inconsistent.

[0020] The foregoing and other aspects, embodiments and features of the present invention can be more fully understood from the following description in conjunction with the accompanying drawings. Other additional aspects of the present invention, such as the features and / or beneficial effects of the exemplary embodiments, will be apparent from the following description or learned from the practice of the specific embodiments according to the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 This is the effect picture after applying No. 1 biological anti-hair loss and hair growth agent; Figure 2 This is the effect picture after applying No. 2 biological anti-hair loss and hair growth agent; Figure 3 This is the effect picture after applying No. 3 biological anti-hair loss and hair growth agent; Figure 4 This is the effect picture after applying No. 4 biological anti-hair loss and hair growth agent; Figure 5 This is the effect picture after applying No. 5 biological anti-hair loss and hair growth agent; Figure 6 This is the effect picture after applying No. 6 biological anti-hair loss and hair growth agent; Figure 7 This is the effect picture after applying No. 7 biological anti-hair loss and hair growth agent; Figure 8 This is the effect picture after applying No. 8 biological anti-hair loss and hair growth agent; Fig. 9 This is the effect picture after applying No. 9 biological anti-hair loss and hair growth agent; Fig.10 This is a comparison chart of the effects after applying No. 5 biological anti-hair loss and hair growth agent and minoxidil. DETAILED DESCRIPTION

[0022] In order to make the purpose, technical solution and advantages of the embodiment of the present invention clearer, the technical solution of the embodiment of the present invention will be clearly and completely described below in conjunction with the drawings of the embodiment of the present invention. Obviously, the described embodiment is a part of the embodiment of the present invention, not all of the embodiments. Based on the described embodiment of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention. Unless otherwise defined, the technical terms or scientific terms used herein should be the common meanings understood by people with ordinary skills in the field to which the present invention belongs.

[0023] The words "first", "second" and similar words used in the patent application specification and claims of the present invention do not indicate any order, quantity or importance, but are only used to distinguish different components. Similarly, unless the context clearly indicates otherwise, the singular form of "a", "an" or "the" and similar words do not indicate a quantity limitation, but indicate the existence of at least one. "Include" or "comprise" and similar words mean that the elements or objects appearing before "include" or "comprise" include the features, wholes, steps, operations, elements and / or components listed after "include" or "comprise", and do not exclude the existence or addition of one or more other features, wholes, steps, operations, elements, components and / or their collections. "Up", "down", "left", "right" and the like are only used to indicate relative positional relationships. When the absolute position of the described object changes, the relative positional relationship may also change accordingly.

[0024] In the examples and comparative examples of the present invention, the raw materials used are all common and general raw materials and reagents on the market.

[0025] The biosynthetic Chinese herbal anti-hair loss and hair growth agent was verified using a mouse model, and the specific method is as follows: first, several male mice were weighed in turn using an analytical balance, and anesthesia was injected at a weight / anesthetic ratio of 10 / 1 (M / V). After the anesthetic takes effect, the hair on the back of the mouse was roughly shaved with an electric clipper, and the hair on the back of the mouse that was not completely removed was continued to be applied with a depilatory cream. After 3-5 minutes, the hair on the back of the mouse was completely wiped clean with physiological saline. Testosterone was applied to the back of the depilated mouse every day (application amount 0.1mL / cm2, 0.2mg per mouse) to establish an androgenic alopecia mouse model, and the application was continued for 28 days. After 7 days of applying the testosterone solution (before treatment), the biological anti-hair loss and hair growth agent of the present invention was used for treatment.

[0026] Control group: Apply testosterone solution (application volume 0.1mL / cm2, 0.2mg per mouse); Biological anti-hair loss and hair growth agent group: Apply testosterone solution (application amount 0.1mL / cm2, 0.2mg per head) and biological anti-hair loss and hair growth agent (3% solution) daily; Testosterone solution was applied daily (for 28 days); 12 hours after the testosterone solution was applied, the biological anti-hair loss agent was applied (for at least 16 days). The hair growth on the back of the mice was observed every day. Example 1

[0027] S1. Grind wolfberry, angelica, notoginseng, glutinous rice, epimedium, Gleditsia sinensis, ginseng, black sesame and Polygonum multiflorum into 5-mesh powder respectively, mix them evenly in equal proportions to form a mixed powder of Chinese herbal medicine.

[0028] S2. Six bacteria, including Pseudomonas, Burkholderia, Bacillus, Candida, Saccharomyces cerevisiae, and Pseudozyma, were cultured in culture medium (yeast powder 2 g / L, peptone 4 g / L, glucose 2 g / L, ammonium nitrate 0.5 g / L, citric acid 0.1 g / L) at 30°C for 36 hours to prepare seed solution.

[0029] Bacterial fermentation part. Prepare basal culture medium (magnesium sulfate 0.2g / L, calcium chloride 0.2g / L, disodium hydrogen phosphate 3.5g / L, potassium dihydrogen phosphate 3.5g / L, ammonium nitrate 5g / L, citric acid 0.2g / L, vitamin B 0.4g / L, lysine 0.2g / L, iron sulfate 0.02g / L, sodium molybdate 0.01g / L, ethanol 0.5g / L), add 5% of Chinese herbal medicine mixed powder as the main carbon source to the basal culture medium, and add 1% grape seed oil at the same time, mix the bacterial seed liquid in the same proportion, inoculate it into the above culture medium at an inoculation rate of 8%, ferment at 37°C for 5 days, and then adjust the temperature to 25°C to continue fermentation for 5 days. Cultivate without ventilation for 4 days, and then cultivate for 6 days at a ventilation rate of 0.2vvm. The stirring speed during the culture process is 160 rpm.

[0030] Yeast fermentation part. Yeast fermentation was carried out at the same time as bacterial fermentation. A basic culture medium (0.2g / L magnesium sulfate, 0.2g / L calcium chloride, 3.5g / L disodium hydrogen phosphate, 3.5g / L potassium dihydrogen phosphate, 5g / L ammonium nitrate, 0.2g / L citric acid, 0.4g / L vitamin C, 0.2g / L lysine, 0.01g / L titanium oxide, 0.5g / L ethanol) was prepared. 5% of Chinese herbal medicine mixed powder was added to the basic culture medium as the main carbon source. At the same time, 1% fructose, rhamnose, mannose, and erythritol were added. The yeast seed liquid was mixed in the same proportion and inoculated into the above culture medium at an inoculation rate of 6%. Fermentation was carried out at 33°C for 7 days, and then the temperature was adjusted to 25°C for another 7 days. The whole process was cultured at a ventilation rate of 0.3vvm. The stirring speed during the culture process was 180 rpm.

[0031] S3, mixed fermentation. The bacterial fermentation liquid and the yeast fermentation liquid were mixed in a mass ratio of 9:1, fermented at 30°C for 3 days, and then the temperature was adjusted to 25°C for another 3 days. The whole process was cultured at a ventilation rate of 0.1vvm for 6 days. The stirring speed during the culture process was 100 rpm.

[0032] After the mixed fermentation of S4 and S3 is completed, stop ventilation and continue stirring; switch to introducing CO2 until the oxygen concentration detected after exhausting the fermentation tank is less than 1%, close the exhaust port, and maintain the pressure in the tank at 0.2Mpa. Maintain the temperature at 35℃ and continue stirring at 200rpm for 1 day. Raise the temperature to 90℃ and maintain it for 8 hours, adjust the pH value to 8, centrifuge at 4000rpm in a three-phase disc centrifuge, filter with a 0.1 um filter bag, ultrafilter, and concentrate to obtain the hair growth agent.

[0033] Using mouse models for validation, the results observed were Figure 1 shown. Example 2

[0034] S1. Grind wolfberry, angelica, notoginseng, glutinous rice, epimedium, Gleditsia sinensis, ginseng, black sesame and Polygonum multiflorum into 10-mesh powder respectively, mix them evenly in equal proportions to form a mixed powder of Chinese herbal medicine.

[0035] S2. Six bacteria, including Pseudomonas, Burkholderia, Bacillus, Candida, Saccharomyces cerevisiae, and Pseudozyma, were cultured in culture medium (yeast powder 2 g / L, peptone 4 g / L, glucose 2 g / L, ammonium nitrate 0.5 g / L, citric acid 0.1 g / L) at 30°C for 48 hours to prepare seed solution.

[0036] Bacterial fermentation part. Prepare basal culture medium (magnesium sulfate 0.2g / L, calcium chloride 0.2g / L, disodium hydrogen phosphate 3.5g / L, potassium dihydrogen phosphate 3.5g / L, ammonium nitrate 5g / L, citric acid 0.2g / L, vitamin B 0.4g / L, lysine 0.2g / L, iron sulfate 0.02g / L, sodium molybdate 0.01g / L, ethanol 0.5g / L), add 10% of Chinese herbal medicine mixed powder as the main carbon source to the basal culture medium, and add 2% grape seed oil at the same time, mix the bacterial seed liquid in the same proportion, inoculate it into the above culture medium at an inoculation rate of 8%, ferment at 37°C for 5 days, and then adjust the temperature to 25°C to continue fermentation for 5 days. Cultivate without ventilation for 4 days, and then cultivate for 6 days at a ventilation rate of 0.2vvm. The stirring speed during the culture process is 160 rpm.

[0037] Yeast fermentation part. Yeast fermentation was carried out at the same time as bacterial fermentation. A basic culture medium (0.2g / L magnesium sulfate, 0.2g / L calcium chloride, 3.5g / L disodium hydrogen phosphate, 3.5g / L potassium dihydrogen phosphate, 5g / L ammonium nitrate, 0.2g / L citric acid, 0.4g / L vitamin C, 0.2g / L lysine, 0.01g / L titanium oxide, 0.5g / L ethanol) was prepared. 10% of Chinese herbal medicine mixed powder was added to the basic culture medium as the main carbon source. At the same time, 2% fructose, rhamnose, mannose, and erythritol were added. The yeast seed liquid was mixed in the same proportion and inoculated into the above culture medium at an inoculation rate of 6%. Fermentation was carried out at 33°C for 7 days, and then the temperature was adjusted to 25°C for another 7 days. The whole process was cultured at a ventilation rate of 0.3vvm. The stirring speed during the culture process was 180 rpm.

[0038] S3, mixed fermentation. The bacterial fermentation liquid and the yeast fermentation liquid were mixed in a mass ratio of 9:1, fermented at 30°C for 3 days, and then the temperature was adjusted to 25°C for another 3 days. The whole process was cultured at a ventilation rate of 0.1vvm for 6 days. The stirring speed during the culture process was 100 rpm.

[0039] After the mixed fermentation of S4 and S3 is completed, stop ventilation and continue stirring; switch to introducing CO2 until the oxygen concentration detected after exhausting the fermentation tank is less than 1%, close the exhaust port, and maintain the pressure in the tank at 0.2Mpa. Maintain the temperature at 35℃ and continue stirring at 200rpm for 1 day. Raise the temperature to 90℃ and maintain it for 8 hours, adjust the pH value to 8, centrifuge at 4000rpm in a three-phase disc centrifuge, filter with a 0.1 um filter bag, ultrafilter, and concentrate to obtain the hair growth agent.

[0040] Using mouse models for validation, the results observed were Figure 2 shown. Example 3

[0041] S1. Grind wolfberry, angelica, notoginseng, glutinous rice, epimedium, Gleditsia sinensis, ginseng, black sesame and Polygonum multiflorum into 10-mesh powder respectively, mix them evenly in equal proportions to form a mixed powder of Chinese herbal medicine.

[0042] S2. Six bacteria, including Pseudomonas, Burkholderia, Bacillus, Candida, Saccharomyces cerevisiae, and Pseudozyma, were cultured in culture medium (yeast powder 2 g / L, peptone 4 g / L, glucose 2 g / L, ammonium nitrate 0.5 g / L, citric acid 0.1 g / L) at 30°C for 48 hours to prepare seed solution.

[0043] Bacterial fermentation part. Prepare basal culture medium (magnesium sulfate 0.2g / L, calcium chloride 0.2g / L, disodium hydrogen phosphate 3.5g / L, potassium dihydrogen phosphate 3.5g / L, ammonium nitrate 5g / L, citric acid 0.2g / L, vitamin B 0.4g / L, lysine 0.2g / L, iron sulfate 0.02g / L, sodium molybdate 0.01g / L, ethanol 0.5g / L), add 15% of Chinese herbal medicine mixed powder as the main carbon source to the basal culture medium, and add 3% grape seed oil at the same time, mix the bacterial seed liquid in the same proportion, inoculate it into the above culture medium according to the inoculation amount of 10%, ferment at 37℃ for 5 days, and then adjust the temperature to 25℃ to continue fermentation for 5 days. Cultivate without ventilation for 4 days, and then cultivate for 6 days at a ventilation rate of 0.2vvm. The stirring speed during the culture process is 160 rpm.

[0044] Yeast fermentation part. Yeast fermentation was carried out at the same time as bacterial fermentation. A basic culture medium (0.2g / L magnesium sulfate, 0.2g / L calcium chloride, 3.5g / L disodium hydrogen phosphate, 3.5g / L potassium dihydrogen phosphate, 5g / L ammonium nitrate, 0.2g / L citric acid, 0.4g / L vitamin C, 0.2g / L lysine, 0.01g / L titanium oxide, 0.5g / L ethanol) was prepared. 15% of Chinese herbal medicine mixed powder was added to the basic culture medium as the main carbon source. At the same time, 2% fructose, rhamnose, mannose, and erythritol were added. The yeast seed liquid was mixed in the same proportion and inoculated into the above culture medium at an inoculation rate of 10%. Fermentation was carried out at 33°C for 7 days, and then the temperature was adjusted to 25°C for another 7 days. The whole process was cultured at a ventilation rate of 0.3vvm. The stirring speed during the culture process was 180 rpm.

[0045] S3, mixed fermentation. The bacterial fermentation liquid and the yeast fermentation liquid were mixed in a mass ratio of 7:3, fermented at 30°C for 3 days, and then the temperature was adjusted to 25°C for another 3 days. The whole process was cultured at a ventilation rate of 0.1 vvm for 6 days. The stirring speed during the culture process was 100 rpm.

[0046] After the mixed fermentation of S4 and S3 is completed, stop ventilation and continue stirring; switch to introducing CO2 until the oxygen concentration detected after exhausting the fermentation tank is less than 1%, close the exhaust port, and maintain the pressure in the tank at 0.2Mpa. Maintain the temperature at 35℃ and continue stirring at 200rpm for 1 day. Raise the temperature to 90℃ and maintain it for 8 hours, adjust the pH value to 8, centrifuge at 4000rpm in a three-phase disc centrifuge, filter with a 0.1 um filter bag, ultrafilter, and concentrate to obtain the hair growth agent.

[0047] Using mouse models for validation, the results observed were Figure 3 shown. Example 4

[0048] S1. Grind wolfberry, angelica, notoginseng, glutinous rice, epimedium, Gleditsia sinensis, ginseng, black sesame and Polygonum multiflorum into 10-mesh powder respectively, mix them evenly in equal proportions to form a mixed powder of Chinese herbal medicine.

[0049] S2. Six bacteria, including Pseudomonas, Burkholderia, Bacillus, Candida, Saccharomyces cerevisiae, and Pseudozyma, were cultured in culture medium (yeast powder 2 g / L, peptone 4 g / L, glucose 2 g / L, ammonium nitrate 0.5 g / L, citric acid 0.1 g / L) at 30°C for 48 hours to prepare seed solution.

[0050] Bacterial fermentation part. Prepare basal culture medium (magnesium sulfate 0.2g / L, calcium chloride 0.2g / L, disodium hydrogen phosphate 3.5g / L, potassium dihydrogen phosphate 3.5g / L, ammonium nitrate 5g / L, citric acid 0.2g / L, vitamin B 0.4g / L, lysine 0.2g / L, iron sulfate 0.02g / L, sodium molybdate 0.01g / L, ethanol 0.5g / L), add 20% of Chinese herbal medicine mixed powder as the main carbon source to the basal culture medium, and add 3% grape seed oil at the same time, mix the bacterial seed liquid in the same proportion, inoculate it into the above culture medium according to the inoculation amount of 10%, ferment at 37℃ for 5 days, and then adjust the temperature to 25℃ to continue fermentation for 5 days. Cultivate without ventilation for 4 days, and then cultivate for 6 days at a ventilation rate of 0.2vvm. The stirring speed during the culture process is 160 rpm.

[0051] Yeast fermentation part. Yeast fermentation was carried out at the same time as bacterial fermentation. A basic culture medium (0.2g / L magnesium sulfate, 0.2g / L calcium chloride, 3.5g / L disodium hydrogen phosphate, 3.5g / L potassium dihydrogen phosphate, 5g / L ammonium nitrate, 0.2g / L citric acid, 0.4g / L vitamin C, 0.2g / L lysine, 0.01g / L titanium oxide, 0.5g / L ethanol) was prepared. 20% of Chinese herbal medicine mixed powder was added to the basic culture medium as the main carbon source. At the same time, 3% fructose, rhamnose, mannose, and erythritol were added. The yeast seed liquid was mixed in the same proportion and inoculated into the above culture medium at an inoculation rate of 10%. Fermentation was carried out at 33°C for 7 days, and then the temperature was adjusted to 25°C for another 7 days. The whole process was cultured at a ventilation rate of 0.3vvm. The stirring speed during the culture process was 180 rpm.

[0052] S3, mixed fermentation. The bacterial fermentation broth and the yeast fermentation broth were mixed in a mass ratio of 6:4, and fermented at 30°C for 3 days, and then the temperature was adjusted to 25°C for another 3 days. The whole process was cultured at a ventilation rate of 0.1 vvm for 6 days. The stirring speed during the culture process was 100 rpm.

[0053] After the mixed fermentation of S4 and S3 is completed, stop ventilation and continue stirring; switch to introducing CO2 until the oxygen concentration detected after exhausting the fermentation tank is less than 1%, close the exhaust port, and maintain the pressure in the tank at 0.2Mpa. Maintain the temperature at 35℃ and continue stirring at 200rpm for 1 day. Raise the temperature to 90℃ and maintain it for 8 hours, adjust the pH value to 8, centrifuge at 4000rpm in a three-phase disc centrifuge, filter with a 0.1 um filter bag, ultrafilter, and concentrate to obtain the hair growth agent.

[0054] Using mouse models for validation, the results observed were Figure 4 shown. Example 5

[0055] S1. Grind wolfberry, angelica, notoginseng, glutinous rice, epimedium, Gleditsia sinensis, ginseng, black sesame and Polygonum multiflorum into 10-mesh powder respectively, mix them evenly in equal proportions to form a mixed powder of Chinese herbal medicine.

[0056] S2. Six bacteria, including Pseudomonas, Burkholderia, Bacillus, Candida, Saccharomyces cerevisiae, and Pseudozyma, were cultured in culture medium (yeast powder 2 g / L, peptone 4 g / L, glucose 2 g / L, ammonium nitrate 0.5 g / L, citric acid 0.1 g / L) at 30°C for 24 hours to prepare seed solutions.

[0057] Bacterial fermentation part. Prepare basal culture medium (magnesium sulfate 0.2g / L, calcium chloride 0.2g / L, disodium hydrogen phosphate 3.5g / L, potassium dihydrogen phosphate 3.5g / L, ammonium nitrate 5g / L, citric acid 0.2g / L, vitamin B 0.4g / L, lysine 0.2g / L, iron sulfate 0.02g / L, sodium molybdate 0.01g / L, ethanol 0.5g / L), add 30% of Chinese herbal medicine mixed powder as the main carbon source to the basal culture medium, and add 3% grape seed oil at the same time, mix the bacterial seed liquid in the same proportion, inoculate it into the above culture medium according to the inoculation amount of 10%, ferment at 37℃ for 5 days, and then adjust the temperature to 25℃ to continue fermentation for 5 days. Cultivate without ventilation for 4 days, and then cultivate for 6 days at a ventilation rate of 0.2vvm. The stirring speed during the culture process is 160 rpm.

[0058] Yeast fermentation part. Yeast fermentation was carried out at the same time as bacterial fermentation. A basic culture medium (0.2g / L magnesium sulfate, 0.2g / L calcium chloride, 3.5g / L disodium hydrogen phosphate, 3.5g / L potassium dihydrogen phosphate, 5g / L ammonium nitrate, 0.2g / L citric acid, 0.4g / L vitamin C, 0.2g / L lysine, 0.01g / L titanium oxide, 0.5g / L ethanol) was prepared. 25% of Chinese herbal medicine mixed powder was added to the basic culture medium as the main carbon source. At the same time, 3% fructose, rhamnose, mannose, and erythritol were added. The yeast seed liquid was mixed in the same proportion and inoculated into the above culture medium at an inoculation rate of 10%. Fermentation was carried out at 33°C for 7 days, and then the temperature was adjusted to 25°C for another 7 days. The whole process was cultured at a ventilation rate of 0.3vvm. The stirring speed during the culture process was 180 rpm.

[0059] S3, mixed fermentation. The bacterial fermentation broth and the yeast fermentation broth were mixed in a mass ratio of 5:5, and fermented at 30°C for 3 days, and then the temperature was adjusted to 25°C for another 3 days. The whole process was cultured at a ventilation rate of 0.1 vvm for 6 days. The stirring speed during the culture process was 100 rpm.

[0060] After the mixed fermentation of S4 and S3 is completed, stop ventilation and continue stirring; switch to introducing CO2 until the oxygen concentration detected after exhausting the fermentation tank is less than 1%, close the exhaust port, and maintain the pressure in the tank at 0.2Mpa. Maintain the temperature at 35℃ and continue stirring at 200rpm for 1 day. Raise the temperature to 90℃ and maintain it for 8 hours, adjust the pH value to 8, centrifuge at 4000rpm in a three-phase disc centrifuge, filter with a 0.1 um filter bag, ultrafilter, and concentrate to obtain the hair growth agent.

[0061] Using mouse models for validation, the results observed were Figure 5 shown. Example 6

[0062] S1. Grind wolfberry, angelica, notoginseng, glutinous rice, epimedium, Gleditsia sinensis, ginseng, black sesame and Polygonum multiflorum into 10-mesh powder respectively, mix them evenly in equal proportions to form a mixed powder of Chinese herbal medicine.

[0063] S2. Six bacteria, including Pseudomonas, Burkholderia, Bacillus, Candida, Saccharomyces cerevisiae, and Pseudozyma, were cultured in culture medium (yeast powder 2 g / L, peptone 4 g / L, glucose 2 g / L, ammonium nitrate 0.5 g / L, citric acid 0.1 g / L) at 33°C for 36 hours to prepare seed solution.

[0064] Bacterial fermentation part. Prepare basal culture medium (magnesium sulfate 0.2g / L, calcium chloride 0.2g / L, disodium hydrogen phosphate 3.5g / L, potassium dihydrogen phosphate 3.5g / L, ammonium nitrate 5g / L, citric acid 0.2g / L, vitamin B 0.4g / L, lysine 0.2g / L, iron sulfate 0.02g / L, sodium molybdate 0.01g / L, ethanol 0.5g / L), add 30% of Chinese herbal medicine mixed powder as the main carbon source to the basal culture medium, and add 4% grape seed oil at the same time, mix the bacterial seed liquid in the same proportion, inoculate it into the above culture medium at an inoculation rate of 6%, ferment at 37°C for 5 days, and then adjust the temperature to 25°C to continue fermentation for 5 days. Cultivate without ventilation for 4 days, and then cultivate for 6 days at a ventilation rate of 0.2vvm. The stirring speed during the culture process is 160 rpm.

[0065] Yeast fermentation part. Yeast fermentation was carried out at the same time as bacterial fermentation. A basic culture medium (0.2g / L magnesium sulfate, 0.2g / L calcium chloride, 3.5g / L disodium hydrogen phosphate, 3.5g / L potassium dihydrogen phosphate, 5g / L ammonium nitrate, 0.2g / L citric acid, 0.4g / L vitamin C, 0.2g / L lysine, 0.01g / L titanium oxide, 0.5g / L ethanol) was prepared. 30% of Chinese herbal medicine mixed powder was added to the basic culture medium as the main carbon source. At the same time, 3% fructose, rhamnose, mannose, and erythritol were added. The yeast seed liquid was mixed in the same proportion and inoculated into the above culture medium at an inoculation rate of 8%. Fermentation was carried out at 33°C for 7 days, and then the temperature was adjusted to 25°C for another 7 days. The whole process was cultured at a ventilation rate of 0.3vvm. The stirring speed during the culture process was 180 rpm.

[0066] S3, mixed fermentation. The bacterial fermentation broth and the yeast fermentation broth were mixed in a mass ratio of 4:6, and fermented at 30°C for 3 days, and then the temperature was adjusted to 25°C for another 3 days. The whole process was cultured at a ventilation rate of 0.1 vvm for 6 days. The stirring speed during the culture process was 100 rpm.

[0067] After the mixed fermentation of S4 and S3 is completed, stop ventilation and continue stirring; switch to introducing CO2 until the oxygen concentration detected after exhausting the fermentation tank is less than 1%, close the exhaust port, and maintain the pressure in the tank at 0.2Mpa. Maintain the temperature at 35℃ and continue stirring at 200rpm for 1 day. Raise the temperature to 90℃ and maintain it for 8 hours, adjust the pH value to 8, centrifuge at 4000rpm in a three-phase disc centrifuge, filter with a 0.1 um filter bag, ultrafilter, and concentrate to obtain the hair growth agent.

[0068] Using mouse models for validation, the results observed were Figure 6 shown. Example 7

[0069] S1. Grind wolfberry, angelica, notoginseng, glutinous rice, epimedium, Gleditsia sinensis, ginseng, black sesame and Polygonum multiflorum into 10-mesh powder respectively, mix them evenly in equal proportions to form a mixed powder of Chinese herbal medicine.

[0070] S2. Six bacteria, including Pseudomonas, Burkholderia, Bacillus, Candida, Saccharomyces cerevisiae, and Pseudozyma, were cultured in culture medium (yeast powder 2 g / L, peptone 4 g / L, glucose 2 g / L, ammonium nitrate 0.5 g / L, citric acid 0.1 g / L) at 33°C for 48 hours to prepare seed solution.

[0071] Bacterial fermentation part. Prepare basal culture medium (magnesium sulfate 0.2g / L, calcium chloride 0.2g / L, disodium hydrogen phosphate 3.5g / L, potassium dihydrogen phosphate 3.5g / L, ammonium nitrate 5g / L, citric acid 0.2g / L, vitamin B 0.4g / L, lysine 0.2g / L, iron sulfate 0.02g / L, sodium molybdate 0.01g / L, ethanol 0.5g / L), add 35% of Chinese herbal medicine mixed powder as the main carbon source to the basal culture medium, and add 4% grape seed oil at the same time, mix the bacterial seed liquid in the same proportion, inoculate it into the above culture medium according to the inoculation amount of 10%, ferment at 37℃ for 5 days, and then adjust the temperature to 25℃ to continue fermentation for 5 days. Cultivate without ventilation for 4 days, and then cultivate for 6 days at a ventilation rate of 0.2vvm. The stirring speed during the culture process is 160 rpm.

[0072] Yeast fermentation part. Yeast fermentation was carried out at the same time as bacterial fermentation. A basic culture medium (0.2g / L magnesium sulfate, 0.2g / L calcium chloride, 3.5g / L disodium hydrogen phosphate, 3.5g / L potassium dihydrogen phosphate, 5g / L ammonium nitrate, 0.2g / L citric acid, 0.4g / L vitamin C, 0.2g / L lysine, 0.01g / L titanium oxide, 0.5g / L ethanol) was prepared. 35% of Chinese herbal medicine mixed powder was added to the basic culture medium as the main carbon source. At the same time, 4% fructose, rhamnose, mannose, and erythritol were added. The yeast seed liquid was mixed in the same proportion and inoculated into the above culture medium at an inoculation rate of 8%. Fermentation was carried out at 33°C for 7 days, and then the temperature was adjusted to 25°C for another 7 days. The whole process was cultured at a ventilation rate of 0.3vvm. The stirring speed during the culture process was 180 rpm.

[0073] S3, mixed fermentation. The bacterial fermentation broth and the yeast fermentation broth were mixed in a mass ratio of 3:7, and fermented at 30°C for 3 days, and then the temperature was adjusted to 25°C for another 3 days. The whole process was cultured at a ventilation rate of 0.1 vvm for 6 days. The stirring speed during the culture process was 100 rpm.

[0074] After the mixed fermentation of S4 and S3 is completed, stop ventilation and continue stirring; switch to introducing CO2 until the oxygen concentration detected after exhausting the fermentation tank is less than 1%, close the exhaust port, and maintain the pressure in the tank at 0.2Mpa. Maintain the temperature at 35℃ and continue stirring at 200rpm for 1 day. Raise the temperature to 90℃ and maintain it for 8 hours, adjust the pH value to 8, centrifuge at 4000rpm in a three-phase disc centrifuge, filter with a 0.1 um filter bag, ultrafilter, and concentrate to obtain the hair growth agent.

[0075] Using mouse models for validation, the results observed were Figure 7 shown. Example 8

[0076] S1. Grind wolfberry, angelica, notoginseng, glutinous rice, epimedium, Gleditsia sinensis, ginseng, black sesame and Polygonum multiflorum into 10-mesh powder respectively, mix them evenly in equal proportions to form a mixed powder of Chinese herbal medicine.

[0077] S2. Six bacteria, including Pseudomonas, Burkholderia, Bacillus, Candida, Saccharomyces cerevisiae, and Pseudozyma, were cultured in culture medium (yeast powder 2 g / L, peptone 4 g / L, glucose 2 g / L, ammonium nitrate 0.5 g / L, citric acid 0.1 g / L) at 35°C for 48 hours to prepare seed solution.

[0078] Bacterial fermentation part. Prepare basal culture medium (magnesium sulfate 0.2g / L, calcium chloride 0.2g / L, disodium hydrogen phosphate 3.5g / L, potassium dihydrogen phosphate 3.5g / L, ammonium nitrate 5g / L, citric acid 0.2g / L, vitamin B 0.4g / L, lysine 0.2g / L, iron sulfate 0.02g / L, sodium molybdate 0.01g / L, ethanol 0.5g / L), add 35% of Chinese herbal medicine mixed powder as the main carbon source to the basal culture medium, and add 2% grape seed oil at the same time, mix the bacterial seed liquid in the same proportion, inoculate it into the above culture medium according to the inoculation amount of 10%, ferment at 37℃ for 5 days, and then adjust the temperature to 25℃ to continue fermentation for 5 days. Cultivate without ventilation for 4 days, and then cultivate for 6 days at a ventilation rate of 0.2vvm. The stirring speed during the culture process is 160 rpm.

[0079] Yeast fermentation part. Yeast fermentation was carried out at the same time as bacterial fermentation. A basic culture medium (0.2g / L magnesium sulfate, 0.2g / L calcium chloride, 3.5g / L disodium hydrogen phosphate, 3.5g / L potassium dihydrogen phosphate, 5g / L ammonium nitrate, 0.2g / L citric acid, 0.4g / L vitamin C, 0.2g / L lysine, 0.01g / L titanium oxide, 0.5g / L ethanol) was prepared. 40% of Chinese herbal medicine mixed powder was added to the basic culture medium as the main carbon source. At the same time, 2% fructose, rhamnose, mannose, and erythritol were added. The yeast seed liquid was mixed in the same proportion and inoculated into the above culture medium at an inoculation rate of 8%. Fermentation was carried out at 33°C for 7 days, and then the temperature was adjusted to 25°C for another 7 days. The whole process was cultured at a ventilation rate of 0.3vvm. The stirring speed during the culture process was 180 rpm.

[0080] S3, mixed fermentation. The bacterial fermentation broth and the yeast fermentation broth were mixed in a mass ratio of 2:8, and fermented at 30°C for 3 days, and then the temperature was adjusted to 25°C for another 3 days. The whole process was cultured at a ventilation rate of 0.1 vvm for 6 days. The stirring speed during the culture process was 100 rpm.

[0081] After the mixed fermentation of S4 and S3 is completed, stop ventilation and continue stirring; switch to introducing CO2 until the oxygen concentration detected after exhausting the fermentation tank is less than 1%, close the exhaust port, and maintain the pressure in the tank at 0.2Mpa. Maintain the temperature at 35℃ and continue stirring at 200rpm for 1 day. Raise the temperature to 90℃ and maintain it for 8 hours, adjust the pH value to 8, centrifuge at 4000rpm in a three-phase disc centrifuge, filter with a 0.1 um filter bag, ultrafilter, and concentrate to obtain the hair growth agent.

[0082] Using mouse models for validation, the results observed were Figure 8 shown. Example 9

[0083] S1. Grind wolfberry, angelica, notoginseng, glutinous rice, epimedium, Gleditsia sinensis, ginseng, black sesame and Polygonum multiflorum into 10-mesh powder respectively, mix them evenly in equal proportions to form a mixed powder of Chinese herbal medicine.

[0084] S2. Six bacteria, including Pseudomonas, Burkholderia, Bacillus, Candida, Saccharomyces cerevisiae, and Pseudozyma, were cultured in culture medium (yeast powder 2 g / L, peptone 4 g / L, glucose 2 g / L, ammonium nitrate 0.5 g / L, citric acid 0.1 g / L) at 35°C for 48 hours to prepare seed solution.

[0085] Bacterial fermentation part. Prepare basal culture medium (magnesium sulfate 0.2g / L, calcium chloride 0.2g / L, disodium hydrogen phosphate 3.5g / L, potassium dihydrogen phosphate 3.5g / L, ammonium nitrate 5g / L, citric acid 0.2g / L, vitamin B 0.4g / L, lysine 0.2g / L, iron sulfate 0.02g / L, sodium molybdate 0.01g / L, ethanol 0.5g / L), add 40% of Chinese herbal medicine mixed powder as the main carbon source to the basal culture medium, and add 3% grape seed oil at the same time, mix the bacterial seed liquid in the same proportion, inoculate it into the above culture medium at an inoculation rate of 8%, ferment at 37°C for 5 days, and then adjust the temperature to 25°C to continue fermentation for 5 days. Cultivate without ventilation for 4 days, and then cultivate for 6 days at a ventilation rate of 0.2vvm. The stirring speed during the culture process is 160 rpm.

[0086] Yeast fermentation part. Yeast fermentation was carried out at the same time as bacterial fermentation. A basic culture medium (0.2g / L magnesium sulfate, 0.2g / L calcium chloride, 3.5g / L disodium hydrogen phosphate, 3.5g / L potassium dihydrogen phosphate, 5g / L ammonium nitrate, 0.2g / L citric acid, 0.4g / L vitamin C, 0.2g / L lysine, 0.01g / L titanium oxide, 0.5g / L ethanol) was prepared. 40% of Chinese herbal medicine mixed powder was added to the basic culture medium as the main carbon source. At the same time, 4% fructose, rhamnose, mannose, and erythritol were added. The yeast seed liquid was mixed in the same proportion and inoculated into the above culture medium at an inoculation rate of 10%. Fermentation was carried out at 33°C for 7 days, and then the temperature was adjusted to 25°C for another 7 days. The whole process was cultured at a ventilation rate of 0.3vvm. The stirring speed during the culture process was 180 rpm.

[0087] S3, mixed fermentation. The bacterial fermentation liquid and the yeast fermentation liquid were mixed in a mass ratio of 1:9, and fermented at 30°C for 3 days, and then the temperature was adjusted to 25°C for another 3 days. The whole process was cultured at a ventilation rate of 0.1vvm for 6 days. The stirring speed during the culture process was 100 rpm.

[0088] After the mixed fermentation of S4 and S3 is completed, stop ventilation and continue stirring; switch to introducing CO2 until the oxygen concentration detected after exhausting the fermentation tank is less than 1%, close the exhaust port, and maintain the pressure in the tank at 0.2Mpa. Maintain the temperature at 35℃ and continue stirring at 200rpm for 1 day. Raise the temperature to 90℃ and maintain it for 8 hours, adjust the pH value to 8, centrifuge at 4000rpm in a three-phase disc centrifuge, filter with a 0.1 um filter bag, ultrafilter, and concentrate to obtain the hair growth agent.

[0089] Using mouse models for validation, the results observed were Fig. 9 shown.

[0090] Comparative Example 1

[0091] In order to further illustrate the effect of the biological anti-hair loss and hair growth agent of the present invention, minoxidil was used as a control to conduct an effect comparison test.

[0092] Control group: Apply testosterone solution (application volume 0.1mL / cm2, 0.2mg per mouse); Biological anti-hair loss and hair growth agent group: Apply testosterone solution (application amount 0.1mL / cm2, 0.2mg per head) and No. 5 biological anti-hair loss and hair growth agent (3% solution) daily; Minoxidil group: Apply testosterone solution (0.1 mL / cm2, 0.2 mg per mouse) and minoxidil (5% solution) daily; Testosterone solution was applied daily (for 28 days); biological anti-hair loss agent or minoxidil was applied 12 hours after testosterone solution application (for at least 16 days). The hair growth on the back of mice was observed every day.

[0093] Using mouse models for validation, the results observed were Fig.10 shown.

[0094] From the observation results of Examples 1-9 and Comparative Example 1, it can be seen that the biosynthetic Chinese herbal anti-hair loss and hair growth agent prepared by the present invention has obvious hair growth promoting ability, and compared with the blank group and minoxidil, it has more obvious hair growth promoting ability. It can be seen that the biological anti-hair loss and hair growth agent can replace minoxidil as an external anti-hair loss and hair growth agent, and has good application prospects.

[0095] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A biosynthetic Chinese herbal anti-hair loss and hair growth agent, characterized in that: The biosynthetic Chinese herbal anti-hair loss and hair growth agent comprises the following effective components: Pseudomonas subtilis fermentation extract and yeast fermentation extract.

2. The biosynthetic Chinese herbal anti-hair loss and hair growth agent according to claim 1, characterized in that: The fermentation raw materials of the Pseudomonas subtilis fermentation extract include any one or more of the Chinese herbal medicines selected from wolfberry, angelica, notoginseng, glutinous rice, epimedium, Gleditsia sinensis, ginseng, black sesame and Polygonum multiflorum.

3. The biosynthetic Chinese herbal anti-hair loss and hair growth agent according to claim 1, characterized in that: The fermentation raw materials of the yeast fermentation extract include any one or more of the Chinese herbal medicines of wolfberry, angelica, notoginseng, glutinous rice, epimedium, Gleditsia sinensis, ginseng, black sesame and Polygonum multiflorum.

4. The biosynthetic Chinese herbal anti-hair loss and hair growth agent according to claim 1, characterized in that: The mass fraction of the Pseudomonas subtilis fermentation extract is 10-90%, and the mass fraction of the yeast fermentation extract is 10-90%.

5. The biosynthetic Chinese herbal anti-hair loss and hair growth agent according to claim 2, characterized in that: The microorganisms used in the Pseudomonas fermentation extract include any one or more of Pseudomonas, Burkholderia, and Bacillus.

6. The biosynthetic Chinese herbal anti-hair loss and hair growth agent according to claim 3, characterized in that: The microorganisms used in the yeast fermentation extract include any one or more of Candida, Saccharomyces cerevisiae and Saccharomyces pyogenes.

7. The method for preparing the biosynthetic Chinese herbal anti-hair loss and hair growth agent according to claims 1-6, characterized in that: The preparation method specifically comprises the following steps: S1. Using Chinese medicinal herbs such as wolfberry, angelica, notoginseng, glutinous rice, epimedium, honey locust thorn, ginseng, black sesame and polygonum multiflorum as the main carbon source, grinding them into powder according to proportion, mixing with grape seed oil, adding nitrate and phosphate inorganic culture components, and fermenting with microorganisms used for Pseudomonas subtilis fermentation extract at 20-40°C for 7-10 days; S2, using Chinese herbal medicines such as wolfberry, angelica, notoginseng, glutinous rice, epimedium, honey locust thorn, ginseng, black sesame and polygonum multiflorum as the main carbon source, grinding them into powder according to proportion, mixing with fructose, rhamnose, mannose and erythritol, adding nitrate and phosphate inorganic culture components, and fermenting with microorganisms used for yeast fermentation extract at 20-40°C for 10-14 days; S3, mixing the fermentation liquid obtained from S1 and S2 according to the proportion, and continuing to ferment at 25-35° C. for 3-7 days; S4. The mixed fermentation liquid obtained in step S3 is cultured for one day by introducing CO2 and stirred at high temperature for 8 hours, and then the pH is adjusted to be greater than 7, and then centrifuged, filtered and concentrated to obtain a hair growth agent.

8. The method for preparing the biosynthetic Chinese herbal anti-hair loss and hair growth agent according to claim 7, characterized in that: The mixing ratio of the fermentation broth obtained by S1 and S2 in step S3 is 1:9-9:1 by mass.

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